David A Hume

Summary

Affiliation: University of Queensland
Country: Australia

Publications

  1. pmc Alternate transcription of the Toll-like receptor signaling cascade
    Christine A Wells
    Eskitis Institute for Cell and Molecular Therapies, School of Biological and Biomedical Sciences, Griffith University, Brisbane 4111, Australia
    Genome Biol 7:R10. 2006
  2. pmc Identification of a non-purple tartrate-resistant acid phosphatase: an evolutionary link to Ser/Thr protein phosphatases?
    Kieran S Hadler
    School of Molecular and Microbial Sciences, The University of Queensland, St, Lucia, 4072, Australia
    BMC Res Notes 1:78. 2008
  3. pmc Computational promoter analysis of mouse, rat and human antimicrobial peptide-coding genes
    Manisha Brahmachary
    Knowledge Extraction Laboratory, Institute for Infocomm Research, 21 Heng Mui Keng Terrace, Singapore 119613, Singapore
    BMC Bioinformatics 7:S8. 2006
  4. ncbi request reprint Interferon-gamma: an overview of signals, mechanisms and functions
    Kate Schroder
    Institute for Molecular Bioscience, University of Queensland, St Lucia, Brisbane 4072, Australia
    J Leukoc Biol 75:163-89. 2004
  5. ncbi request reprint The mononuclear phagocyte system
    David A Hume
    Institute for Molecular Bioscience, University of Queensland, Q4072, Australia
    Curr Opin Immunol 18:49-53. 2006
  6. ncbi request reprint Therapeutic targets in inflammatory disease
    David A Hume
    Institute for Molecular Bioscience, University of Queensland, Brisbane, QLD 4072, Australia
    Curr Med Chem 12:2925-9. 2005
  7. ncbi request reprint The mononuclear phagocyte system revisited
    David A Hume
    Institute for Molecular Bioscience, University of Queensland, Australia
    J Leukoc Biol 72:621-7. 2002
  8. doi request reprint Microphthalmia transcription factor regulates the expression of the novel osteoclast factor GPNMB
    Vera M Ripoll
    Institute for Molecular Biosciences, Co operative Research Centre for Chronic Inflammatory Diseases, The University of Queensland, St Lucia, QLD 4072, Australia
    Gene 413:32-41. 2008
  9. ncbi request reprint Overview of the pipeline for structural and functional characterization of macrophage proteins at the university of queensland
    Weining Meng
    School of Molecular and Microbial Sciences, University of Queensland, Brisbane, Australia
    Methods Mol Biol 426:577-87. 2008
  10. pmc Genetic control of the innate immune response
    Christine A Wells
    Institute for Molecular Biosciences, The University of Queensland, Brisbane, QLD, 4072, Australia
    BMC Immunol 4:5. 2003

Detail Information

Publications92

  1. pmc Alternate transcription of the Toll-like receptor signaling cascade
    Christine A Wells
    Eskitis Institute for Cell and Molecular Therapies, School of Biological and Biomedical Sciences, Griffith University, Brisbane 4111, Australia
    Genome Biol 7:R10. 2006
    ..Functional annotation of variant proteins was assessed in light of inflammatory signaling in mouse primary macrophages, and the expression of each variant transcript was assessed by splicing arrays...
  2. pmc Identification of a non-purple tartrate-resistant acid phosphatase: an evolutionary link to Ser/Thr protein phosphatases?
    Kieran S Hadler
    School of Molecular and Microbial Sciences, The University of Queensland, St, Lucia, 4072, Australia
    BMC Res Notes 1:78. 2008
    ..Due to its role in bone resorption the 35 kDa TRAcP has become a promising target for the development of anti-osteoporotic chemotherapeutics...
  3. pmc Computational promoter analysis of mouse, rat and human antimicrobial peptide-coding genes
    Manisha Brahmachary
    Knowledge Extraction Laboratory, Institute for Infocomm Research, 21 Heng Mui Keng Terrace, Singapore 119613, Singapore
    BMC Bioinformatics 7:S8. 2006
    ..For many AMPcgs the promoter elements and transcription factors that control their tissue cell-specific expression have yet to be fully identified and characterized...
  4. ncbi request reprint Interferon-gamma: an overview of signals, mechanisms and functions
    Kate Schroder
    Institute for Molecular Bioscience, University of Queensland, St Lucia, Brisbane 4072, Australia
    J Leukoc Biol 75:163-89. 2004
    ..In addition, integration of signaling and response with other cytokines and pathogen-associated molecular patterns, such as tumor necrosis factor-alpha, interleukin-4, type I IFNs, and lipopolysaccharide are discussed...
  5. ncbi request reprint The mononuclear phagocyte system
    David A Hume
    Institute for Molecular Bioscience, University of Queensland, Q4072, Australia
    Curr Opin Immunol 18:49-53. 2006
    ..The concept of the MPS may have partly outlived its usefulness...
  6. ncbi request reprint Therapeutic targets in inflammatory disease
    David A Hume
    Institute for Molecular Bioscience, University of Queensland, Brisbane, QLD 4072, Australia
    Curr Med Chem 12:2925-9. 2005
    ..It also highlights important limitations in the validation of inflammatory drug targets, and in the rate of discovery and development of new antiinflammatory drugs...
  7. ncbi request reprint The mononuclear phagocyte system revisited
    David A Hume
    Institute for Molecular Bioscience, University of Queensland, Australia
    J Leukoc Biol 72:621-7. 2002
    ..We discuss the origins of macrophage heterogeneity and argue that macrophages and antigen-representing dendritic cells are closely related and part of the MPS...
  8. doi request reprint Microphthalmia transcription factor regulates the expression of the novel osteoclast factor GPNMB
    Vera M Ripoll
    Institute for Molecular Biosciences, Co operative Research Centre for Chronic Inflammatory Diseases, The University of Queensland, St Lucia, QLD 4072, Australia
    Gene 413:32-41. 2008
    ..The inclusion of gpnmb in the MITF regulon suggests a role for GPNMB in mature osteoclast function...
  9. ncbi request reprint Overview of the pipeline for structural and functional characterization of macrophage proteins at the university of queensland
    Weining Meng
    School of Molecular and Microbial Sciences, University of Queensland, Brisbane, Australia
    Methods Mol Biol 426:577-87. 2008
    ..Jointly, the data sheds light on the molecular and cellular functions of macrophage proteins...
  10. pmc Genetic control of the innate immune response
    Christine A Wells
    Institute for Molecular Biosciences, The University of Queensland, Brisbane, QLD, 4072, Australia
    BMC Immunol 4:5. 2003
    ....
  11. ncbi request reprint PU.1 and ICSBP control constitutive and IFN-gamma-regulated Tlr9 gene expression in mouse macrophages
    Kate Schroder
    Special Research Centre for Functional and Applied Genomics, Institute for Molecular Bioscience, University of Queensland, St Lucia, Brisbane 4072, Australia
    J Leukoc Biol 81:1577-90. 2007
    ..This study therefore characterizes the regulation of mouse Tlr9 expression and defines a molecular mechanism by which IFN-gamma amplifies mouse macrophage responses to CpG DNA...
  12. doi request reprint A conserved distal segment of the mouse CSF-1 receptor promoter is required for maximal expression of a reporter gene in macrophages and osteoclasts of transgenic mice
    Dmitry A Ovchinnikov
    Institute for Molecular Bioscience, University of Queensland, Australia
    J Leukoc Biol 87:815-22. 2010
    ..RT-PCR analyses of Csf1r mRNA revealed that mouse OCL use another promoter within this region, distinct from that used in placental trophoblasts, to generate an alternative 5'UTR...
  13. pmc Structural basis for recruitment of tandem hotdog domains in acyl-CoA thioesterase 7 and its role in inflammation
    Jade K Forwood
    School of Molecular and Microbial Sciences, University of Queensland, Brisbane, Queensland 4072, Australia
    Proc Natl Acad Sci U S A 104:10382-7. 2007
    ..Together, the results link the molecular and cellular functions of Acot7 and identify the enzyme as a candidate drug target in inflammatory disease...
  14. ncbi request reprint Expression of Gal4-dependent transgenes in cells of the mononuclear phagocyte system labeled with enhanced cyan fluorescent protein using Csf1r-Gal4VP16/UAS-ECFP double-transgenic mice
    Dmitry A Ovchinnikov
    Institute for Molecular Bioscience, Bldg 80, University of Queensland, Brisbane, Queensland 4072, Australia
    J Leukoc Biol 83:430-3. 2008
    ..The new mouse line provides a useful tool for overexpression of transgenes in cells of the myeloid lineage, while simultaneously labeling them by ECFP expression...
  15. pmc Continued discovery of transcriptional units expressed in cells of the mouse mononuclear phagocyte lineage
    Christine A Wells
    Institute for Molecular Bioscience and ARC Special Research Centre for Functional and Applied Genomics, Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland 4072, Australia
    Genome Res 13:1360-5. 2003
    ....
  16. ncbi request reprint CpG DNA activates survival in murine macrophages through TLR9 and the phosphatidylinositol 3-kinase-Akt pathway
    David P Sester
    Cooperative Research Centre for Chronic Inflammatory Diseases and Special Research Centre for Functional and Applied Genomics, Institute for Molecular Bioscience, University of Queensland, St Lucia, Brisbane, QLD 4072, Australia
    J Immunol 177:4473-80. 2006
    ..Therefore, CpG DNA activates macrophage survival via TLR9 and the PI3K-Akt pathway and independently of DNA-PK and MEK-ERK...
  17. ncbi request reprint LPS regulates proinflammatory gene expression in macrophages by altering histone deacetylase expression
    Hnin Thanda Aung
    Cooperative Research Centre for Chronic Inflammatory Diseases, Institute for Molecular Bioscience, University of Queensland, Australia
    FASEB J 20:1315-27. 2006
    ..1 degradation. Hence, HDACs act as potent and selective negative regulators of proinflammatory gene expression and act to prevent excessive inflammatory responses in macrophages...
  18. ncbi request reprint The JNK are important for development and survival of macrophages
    S Roy Himes
    Cooperative Research Centre for Chronic Inflammatory Disease, Institute for Molecular Biosciences, University of Queensland, Brisbane, Australia
    J Immunol 176:2219-28. 2006
    ..This pattern of expression may underlie the novel role of JNK in macrophages...
  19. ncbi request reprint Gpnmb is induced in macrophages by IFN-gamma and lipopolysaccharide and acts as a feedback regulator of proinflammatory responses
    Vera M Ripoll
    Cooperative Research Centre for Chronic Inflammatory Diseases and Special Research Centre for Functional and Applied Genomics, Institute for Molecular Bioscience, University of Queensland, Queensland, Australia
    J Immunol 178:6557-66. 2007
    ..Thus, GPNMB acts as a negative regulator of macrophage inflammatory responses...
  20. ncbi request reprint The Ewing sarcoma protein (EWS) binds directly to the proximal elements of the macrophage-specific promoter of the CSF-1 receptor (csf1r) gene
    David A Hume
    Australian Research Council Special Research Centre for Functional and Applied Genomics and the Cooperative Research Centre for Chronic Inflammatory Diseases, University of Queensland, Brisbane, Queensland, Australia
    J Immunol 180:6733-42. 2008
    ..Transfection assays suggest that EWS does not act as a conventional transcriptional activator or repressor. We hypothesize that EWS contributes to start site recognition in TATA-less mammalian promoters...
  21. ncbi request reprint LPS regulates a set of genes in primary murine macrophages by antagonising CSF-1 action
    David P Sester
    Cooperative Research Centre for Chronic Inflammatory Diseases, Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland 4072, Australia
    Immunobiology 210:97-107. 2005
    ..This finding also provides a biological relevance to the well-documented ability of macrophage activators to down-modulate surface expression of the CSF-1R...
  22. pmc Systematic characterization of the zinc-finger-containing proteins in the mouse transcriptome
    Timothy Ravasi
    Institute for Molecular Bioscience, Brisbane, Australia
    Genome Res 13:1430-42. 2003
    ....
  23. ncbi request reprint A medium or high throughput protein refolding assay
    Nathan P Cowieson
    Institute for Molecular Bioscience, University of Queensland, Brisbane, Australia
    Methods Mol Biol 426:269-75. 2008
    ..Two formats of the assay are described, a manual assay that is useful for screening small numbers of targets, and an automated implementation that is useful for large numbers of targets...
  24. ncbi request reprint Differential effects of CpG DNA on IFN-beta induction and STAT1 activation in murine macrophages versus dendritic cells: alternatively activated STAT1 negatively regulates TLR signaling in macrophages
    Kate Schroder
    Cooperative Research Centre for Chronic Inflammatory Diseases and Special Research Centre for Functional and Applied Genomics, Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland, Australia
    J Immunol 179:3495-503. 2007
    ..The differential activation of Ifn-beta and STAT1 by CpG DNA in mouse macrophages vs dendritic cells provides a likely mechanism for their divergent roles in priming the adaptive immune response...
  25. doi request reprint Colony-stimulating factor-1 (CSF-1) delivers a proatherogenic signal to human macrophages
    Katharine M Irvine
    The University of Queensland, Institute for Molecular Bioscience, Brisbane, Queensland, Australia
    J Leukoc Biol 85:278-88. 2009
    ....
  26. ncbi request reprint Differential effects of selective HDAC inhibitors on macrophage inflammatory responses to the Toll-like receptor 4 agonist LPS
    Maria A Halili
    The University of Queensland, Institute for Molecular Bioscience, S Lucia, Brisbane, Queensland, 4072, Australia
    J Leukoc Biol 87:1103-14. 2010
    ..Thus, 17a provides a tool to identify individual HDACs with proinflammatory properties...
  27. doi request reprint CD148/DEP-1 association with areas of cytoskeletal organisation in macrophages
    Richa K Dave
    Institute for Molecular Bioscience, The University of Queensland, St Lucia, QLD, Australia
    Exp Cell Res 315:1734-44. 2009
    ..There were no detectable effects on the CSF-1 receptor-akt signalling pathway. These results are consistent with the hypothesis that CD148 is a regulator of macrophage activity...
  28. ncbi request reprint Human tartrate-resistant acid phosphatase becomes an effective ATPase upon proteolytic activation
    Natasa Mitic
    School of Molecular and Microbial Sciences, The University of Queensland, St Lucia, Australia
    Arch Biochem Biophys 439:154-64. 2005
    ..Notably, at low pH this residue may act as a proton donor for the leaving group. In this respect the mechanism of cleaved TRAcP resembles that of sweet potato purple acid phosphatase...
  29. ncbi request reprint Osteal tissue macrophages are intercalated throughout human and mouse bone lining tissues and regulate osteoblast function in vitro and in vivo
    Ming K Chang
    Institute for Molecular Bioscience, Cooperative Research Centre for Chronic Inflammatory Diseases, University of Queensland, Brisbane, Queensland, Australia
    J Immunol 181:1232-44. 2008
    ..These observations implicate OsteoMacs, in addition to osteoclasts and osteoblasts, as principal participants in bone dynamics...
  30. ncbi request reprint Mouse neutrophilic granulocytes express mRNA encoding the macrophage colony-stimulating factor receptor (CSF-1R) as well as many other macrophage-specific transcripts and can transdifferentiate into macrophages in vitro in response to CSF-1
    R Tedjo Sasmono
    CRC for Chronic Inflammatory Diseases and ARC Special Research Centre for Functional and Applied Genomics, Institute for Molecular Bioscience, The University of Queensland, St Lucia, Australia
    J Leukoc Biol 82:111-23. 2007
    ..In keeping with this hypothesis, we showed that purified Ly-6G-positive granulocytes express CSF-1R after overnight culture and can subsequently differentiate to form F4/80-positive macrophages in response to CSF-1...
  31. pmc Conservation and divergence in Toll-like receptor 4-regulated gene expression in primary human versus mouse macrophages
    Kate Schroder
    Institute for Molecular Bioscience, University of Queensland, Brisbane 4072, Australia
    Proc Natl Acad Sci U S A 109:E944-53. 2012
    ..Thus, the genes controlled by complex, highly conserved promoters that facilitate dynamic regulation are also the most susceptible to evolutionary change...
  32. pmc Macrophage activation and differentiation signals regulate schlafen-4 gene expression: evidence for Schlafen-4 as a modulator of myelopoiesis
    Wendy J van Zuylen
    The University of Queensland, Institute for Molecular Bioscience, Queensland, Australia
    PLoS ONE 6:e15723. 2011
    ..Little is known of their function, but previous studies suggest roles in immune cell development. In this report, we assessed Slfn regulation and function in macrophages, which are key cellular regulators of innate immunity...
  33. ncbi request reprint Protein structure determination using a combination of cross-linking, mass spectrometry, and molecular modeling
    Dmitri Mouradov
    School of Molecular and Microbial Sciences, University of Queensland, Brisbane, Australia
    Methods Mol Biol 426:459-74. 2008
    ....
  34. ncbi request reprint Differences in macrophage activation by bacterial DNA and CpG-containing oligonucleotides
    Tara L Roberts
    Institute for Molecular Bioscience, University of Queensland, Brisbane, Australia
    J Immunol 175:3569-76. 2005
    ..The length dependence of the CpG ODN response was found to correlate with the presence in macrophages of a length-dependent uptake process for DNA. This transport system was absent from B cells and fibroblasts...
  35. ncbi request reprint A CSF-1 receptor kinase inhibitor targets effector functions and inhibits pro-inflammatory cytokine production from murine macrophage populations
    Katharine M Irvine
    Cooperative Research Centre for Chronic Inflammatory Diseases and Special Research Centre for Functional and Applied Genomics, Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland, Australia
    FASEB J 20:1921-3. 2006
    ..CSF-1R inhibitors such as CYC10268 provide a powerful tool to dissect the role of the CSF-1/CSF-1R signaling system in a range of biological systems and have potential for a number of therapeutic applications...
  36. ncbi request reprint A general target selection method for crystallographic proteomics
    Gautier Robin
    Institute for Molecular Bioscience, University of Queensland, Brisbane, Australia
    Methods Mol Biol 426:27-35. 2008
    ..This novel procedure was designed to maximize selection efficiency, and its prevailing criteria categories make it suitable for a broad range of structural proteomics projects...
  37. pmc Histone deacetylase 7 promotes Toll-like receptor 4-dependent proinflammatory gene expression in macrophages
    Melanie R Shakespear
    Institute for Molecular Bioscience and Australian Infectious Diseases Research Centre, University of Queensland, Queensland 4072, Australia
    J Biol Chem 288:25362-74. 2013
    ..Thus, Hdac7-u positively regulates HIF-1α-dependent TLR signaling in macrophages, whereas an interaction with CtBP1 likely prevents Hdac7-s from exerting this effect. Hdac7 may represent a potential inflammatory disease target. ..
  38. doi request reprint HIN-200 proteins regulate caspase activation in response to foreign cytoplasmic DNA
    Tara L Roberts
    The University of Queensland, Institute for Molecular Bioscience, QLD 4072, Australia
    Science 323:1057-60. 2009
    ..This work indicates that HIN-200 proteins can act as pattern recognition receptors mediating responses to cytoplasmic dsDNA...
  39. doi request reprint A rescue strategy for multimapping short sequence tags refines surveys of transcriptional activity by CAGE
    Geoffrey J Faulkner
    The Institute for Molecular Bioscience, University of Queensland, Brisbane, QLD 4072, Australia
    Genomics 91:281-8. 2008
    ..These results suggest that the multimap tags produced by high-throughput, short sequence tag-based approaches can be rescued to augment greatly the transcriptome coverage provided by single-map tags alone...
  40. ncbi request reprint Characterisation and trophic functions of murine embryonic macrophages based upon the use of a Csf1r-EGFP transgene reporter
    Fiona Rae
    Institute for Molecular Bioscience and ARC Special Research Centre for Functional and Applied Genomics, University of Queensland, Brisbane, Queensland 4072, Australia
    Dev Biol 308:232-46. 2007
    ..These findings suggest a trophic role of macrophages in embryonic kidney development, which may continue to play a similar role in postnatal repair...
  41. doi request reprint An antibody against the colony-stimulating factor 1 receptor depletes the resident subset of monocytes and tissue- and tumor-associated macrophages but does not inhibit inflammation
    Kelli P A MacDonald
    Queensland Institute of Medical Research, Brisbane, Australia
    Blood 116:3955-63. 2010
    ..The data indicate that CSF1R signaling is required only for the maturation and replacement of resident-type monocytes and tissue macrophages, and is not required for monocyte production or inflammatory function...
  42. ncbi request reprint Transcriptional regulatory networks in macrophages
    David A Hume
    ARC Special Research Centre for Functional and Applied Genomics and CRC for Chronic Inflammatory Diseases, Institute for Molecular Bioscience, University of Queensland, Australia
    Novartis Found Symp 281:2-18; discussion 18-24, 50-3, 208-9. 2007
    ....
  43. doi request reprint TLR9-independent effects of inhibitory oligonucleotides on macrophage responses to S. typhimurium
    Angela Trieu
    The University of Queensland, Institute for Molecular Bioscience, Queensland, Australia
    Immunol Cell Biol 87:218-25. 2009
    ....
  44. ncbi request reprint Incorporating a TEV cleavage site reduces the solubility of nine recombinant mouse proteins
    Mareike Kurz
    Institute for Molecular Bioscience, University of Queensland, Brisbane, QLD 4072, Australia
    Protein Expr Purif 50:68-73. 2006
    ..Our work suggests that one needs to be very careful when making modifications to expression vectors and combining different affinity and fusion tags and cleavage sites...
  45. ncbi request reprint Cutting edge: species-specific TLR9-mediated recognition of CpG and non-CpG phosphorothioate-modified oligonucleotides
    Tara L Roberts
    Institute for Molecular Bioscience, Cooperative Research Centre for Chronic Inflammatory Diseases, University of Queensland, Brisbane, Australia
    J Immunol 174:605-8. 2005
    ..Thus, TLR9 may be responsible for mediating many published CpG-independent responses to PS-ODN...
  46. ncbi request reprint Focusing in on structural genomics: the University of Queensland structural biology pipeline
    Munish Puri
    Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland, Australia
    Biomol Eng 23:281-9. 2006
    ..The structures from this pipeline will provide insights into the function of previously uncharacterized macrophage proteins and could lead to the validation of new drug targets for chronic obstructive pulmonary disease and arthritis...
  47. ncbi request reprint CSF-1 as a regulator of macrophage activation and immune responses
    Matthew J Sweet
    CRC for Chronic Inflammatory Diseases, Institute for Molecular Bioscience and Department of Microbiology Parasitology, University of Queensland, QLD 4072, Australia
    Arch Immunol Ther Exp (Warsz) 51:169-77. 2003
    ..This review will summarize our current understanding of the effects of CSF-1 on the activation state of mature macrophages and its role in regulating immune responses...
  48. ncbi request reprint An inflammatory role for the mammalian carboxypeptidase inhibitor latexin: relationship to cystatins and the tumor suppressor TIG1
    Anna Aagaard
    Institute for Molecular Bioscience, University of Queensland, Brisbane, QLD 4072, Australia
    Structure 13:309-17. 2005
    ..The structure of the tumor suppressor protein TIG1 was modeled, revealing its putative membrane binding surface...
  49. ncbi request reprint An automatable screen for the rapid identification of proteins amenable to refolding
    Nathan P Cowieson
    Institute for Molecular Bioscience and ARC Special Research Centre for Functional and Applied Genomics, University of Queensland, Brisbane, Queensland, Australia
    Proteomics 6:1750-7. 2006
    ..The efficacy of the screen is demonstrated on small-scale expression samples for 15 proteins. Refolding is then validated by large-scale expressions using SEC and circular dichroism...
  50. pmc Identification and analysis of chromodomain-containing proteins encoded in the mouse transcriptome
    Khairina Tajul-Arifin
    ARC Special Research Centre for Functional and Applied Genomics, Institute for Molecular Bioscience, University of Queensland, St Lucia, Queensland 4072, Australia
    Genome Res 13:1416-29. 2003
    ..The likely functions of these proteins are discussed in relation to the known functions of other chromodomain-containing proteins within the same family...
  51. ncbi request reprint Generation of diversity in the innate immune system: macrophage heterogeneity arises from gene-autonomous transcriptional probability of individual inducible genes
    Timothy Ravasi
    Institute for Molecular Bioscience, and Cooperative Research Center for Chronic Inflammatory Diseases, University of Queensland, Brisbane, Queensland 4072, Australia
    J Immunol 168:44-50. 2002
    ..The results indicate that each LPS-inducible gene has its own inherent probability of activation in response to LPS...
  52. pmc Selective induction of the Notch ligand Jagged-1 in macrophages by soluble egg antigen from Schistosoma mansoni involves ERK signalling
    Felicia Goh
    Institute for Molecular Bioscience, University of Queensland, QLD, Australia
    Immunology 127:326-37. 2009
    ..Taken together, our data suggest that Jagged-1 is an ERK-dependent target of TLR signalling that has a macrophage-specific function in the response to SEA...
  53. ncbi request reprint The Runx1 transcription factor controls CSF-1-dependent and -independent growth and survival of macrophages
    Stewart R Himes
    CRC for Chronic Inflammatory Disease, Institute for Molecular Biosciences, Queensland Biosciences Precinct, Bldg 80, Services Rd, University of Queensland, Brisbane, Queensland 4072, Australia
    Oncogene 24:5278-86. 2005
    ..These findings suggest that Runx1 regulates growth and survival of myeloid cells and provide a novel insight into the role of Runx family gene translocations in leukemogenesis...
  54. ncbi request reprint Probing the S100 protein family through genomic and functional analysis
    Timothy Ravasi
    SRC for Functional and Applied Genomics, University of Queensland, Brisbabe, OLD, Australia
    Genomics 84:10-22. 2004
    ..Evolution of the S100 proteins appears to have occurred in a modular fashion, also seen in other protein families such as the C2H2-type zinc-finger family...
  55. ncbi request reprint Multiple tissue-specific promoters control expression of the murine tartrate-resistant acid phosphatase gene
    Nicole C Walsh
    Institute for Molecular Bioscience and School of Molecular and Microbial Sciences, University of Queensland, Brisbane, Australia
    Gene 307:111-23. 2003
    ..The distinct expression patterns for each of the TRAP 5'-UTRs suggest that TRAP mRNA expression is regulated by the use of four alternative tissue- and cell-restricted promoters...
  56. doi request reprint The regulated retrotransposon transcriptome of mammalian cells
    Geoffrey J Faulkner
    Institute for Molecular Bioscience, University of Queensland, Australia
    Nat Genet 41:563-71. 2009
    ..We conclude that retrotransposon transcription has a key influence upon the transcriptional output of the mammalian genome...
  57. ncbi request reprint Pilot studies on the parallel production of soluble mouse proteins in a bacterial expression system
    Nathan P Cowieson
    Institute for Molecular Bioscience, Australia
    J Struct Funct Genomics 6:13-20. 2005
    ..This success rate compares favourably with other protein screening projects, particularly for eukaryotic proteins, and could be further improved by modifications at the cloning step...
  58. doi request reprint Beta-arrestin 2 is required for complement C1q expression in macrophages and constrains factor-independent survival
    Jane E Lattin
    The University of Queensland, Institute for Molecular Bioscience, QLD 4072, Australia
    Mol Immunol 47:340-7. 2009
    ..Given that loss of C1q function is strongly associated with the development of systemic lupus erythematosus, ARRB2 may act to limit the development of autoimmune disease...
  59. ncbi request reprint Modification of recombinatorial cloning for small affinity tag fusion protein construct generation
    Pawel Listwan
    School of Molecular and Microbial Sciences, University of Queensland, Brisbane, Queensland 4072, Australia
    Anal Biochem 346:327-9. 2005
  60. ncbi request reprint Modelling the structure of latexin-carboxypeptidase A complex based on chemical cross-linking and molecular docking
    Dmitri Mouradov
    School of Molecular and Microbial Sciences, Institute for Molecular Bioscience, Cooperative Research Centre for Chronic Inflammatory Diseases, Department of Mathematics, The University of Queensland, Brisbane, Queensland 4072, Australia
    Protein Eng Des Sel 19:9-16. 2006
    ..m.s. deviation of Calpha atoms of the crystal structure. The study demonstrates that cross-linking in combination with mass spectrometry can lead to efficient and accurate structural modelling of protein complexes...
  61. ncbi request reprint The expression of Clcn7 and Ostm1 in osteoclasts is coregulated by microphthalmia transcription factor
    Nicholas A Meadows
    Institute for Molecular Biosciences, The University of Queensland, St Lucia, Queensland 4072, Australia
    J Biol Chem 282:1891-904. 2007
    ....
  62. doi request reprint Identification of disulfide-containing chemical cross-links in proteins using MALDI-TOF/TOF-mass spectrometry
    Gordon J King
    Cooperative Research Centre for Chronic Inflammatory Diseases, Institute for Molecular Bioscience, University of Queensland, St Lucia Brisbane
    Anal Chem 80:5036-43. 2008
    ..The methodology we report is robust and amenable to automation, and permits the analysis of native cystines along with those introduced by disulfide-containing cross-linkers...
  63. ncbi request reprint A macrophage colony-stimulating factor receptor-green fluorescent protein transgene is expressed throughout the mononuclear phagocyte system of the mouse
    R Tedjo Sasmono
    Institute for Molecular Bioscience and ARC Special Research Centre for Functional and Applied Genomics, University of Queensland, Brisbane, Australia
    Blood 101:1155-63. 2003
    ..We have therefore defined the elements required to generate myeloid- and trophoblast-specific transgenes as well as a model system for the study of mononuclear phagocyte development and function...
  64. doi request reprint The immunostimulatory activity of phosphorothioate CpG oligonucleotides is affected by distal sequence changes
    Tara L Roberts
    The University of Queensland, Institute for Molecular Bioscience, QLD 4072, Australia
    Mol Immunol 48:1027-34. 2011
    ..The reduction in immunostimulatory activity of PS-ODN was associated with a delay in the activation of MAP kinases...
  65. ncbi request reprint Syntaxin 6 and Vti1b form a novel SNARE complex, which is up-regulated in activated macrophages to facilitate exocytosis of tumor necrosis Factor-alpha
    Rachael Z Murray
    Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland, Australia 4072
    J Biol Chem 280:10478-83. 2005
    ..This study shows how macrophages have uniquely adapted a novel Golgi-associated SNARE complex to accommodate their requirement for increased cytokine secretion...
  66. pmc Experimental validation of the regulated expression of large numbers of non-coding RNAs from the mouse genome
    Timothy Ravasi
    ARC Special Research Centre for Functional and Applied Genomics, Institute for Molecular Bioscience, University of Queensland, Brisbane QLD 4072, Australia
    Genome Res 16:11-9. 2006
    ..Taken together, the data provide strong support for the conclusion that ncRNAs are an important, regulated component of the mammalian transcriptome...
  67. ncbi request reprint Osteal macrophages: a new twist on coupling during bone dynamics
    Allison R Pettit
    The University of Queensland, Institute for Molecular Bioscience, Cooperative Research Centre for Chronic Inflammatory Diseases, Brisbane, QLD 4072, Australia
    Bone 43:976-82. 2008
    ..This review discusses the wider impact of macrophages in bone homeostasis and disease and proposes novel roles for OsteoMacs in bone modelling and remodelling...
  68. doi request reprint Tiny RNAs associated with transcription start sites in animals
    Ryan J Taft
    Institute for Molecular Bioscience, The University of Queensland, St Lucia, Australia
    Nat Genet 41:572-8. 2009
    ..tiRNAs are generally, although not exclusively, associated with highly expressed transcripts and sites of RNA polymerase II binding. We suggest that tiRNAs may be a general feature of transcription in metazoa and possibly all eukaryotes...
  69. ncbi request reprint G-protein-coupled receptor expression, function, and signaling in macrophages
    Jane Lattin
    Cooperative Research Centre for Chronic Inflammatory Diseases, University of Queensland, St Lucia, Brisbane, QLD 4072, Australia
    J Leukoc Biol 82:16-32. 2007
    ..In particular, we examine the crosstalk between GPCR and TLR signaling pathways and highlight GPCR signaling molecules which are likely to have uncharacterized functions in this cell lineage...
  70. ncbi request reprint The molecular basis for the lack of immunostimulatory activity of vertebrate DNA
    Katryn J Stacey
    Cooperative Research Center for Chronic Inflammatory Diseases, Institute for Molecular Bioscience, School of Molecular and Microbial Sciences, University of Queensland, Brisbane, Australia
    J Immunol 170:3614-20. 2003
    ..The immunostimulatory activity of DNA is determined by the frequency of unmethylated stimulatory sequences within an individual DNA strand and the ratio of stimulatory to inhibitory sequences...
  71. ncbi request reprint Signal integration between IFNgamma and TLR signalling pathways in macrophages
    Kate Schroder
    Institute for Molecular Bioscience, University of Queensland, QLD Bioscience Precinct, Brisbane, QLD 4072, Australia
    Immunobiology 211:511-24. 2006
    ..Synergy occurs at multiple levels, ranging from signal recognition to convergence of signals at the promoters of target genes. In particular, the cross-talk between the IFNgamma, and LPS and CpG DNA signalling pathways is discussed...
  72. pmc The mouse secretome: functional classification of the proteins secreted into the extracellular environment
    Sean M Grimmond
    Institute for Molecular Bioscience and ARC Special Research Centre for Functional and Applied Genomics, University of Queensland, St Lucia 4072, Australia
    Genome Res 13:1350-9. 2003
    ..To highlight the utility of this information, we discuss the CUB domain-containing protein family...
  73. ncbi request reprint Colony-stimulating factor-1 suppresses responses to CpG DNA and expression of toll-like receptor 9 but enhances responses to lipopolysaccharide in murine macrophages
    Matthew J Sweet
    Department of Immunology and Bacteriology, University of Glasgow, Glasgow, United Kingdom
    J Immunol 168:392-9. 2002
    ..Hence, CSF-1 may regulate host responses to pathogens through modulation of TLR expression. Furthermore, these results suggest that CSF-1 and CSF-1R antagonists may enhance the efficacy of CpG DNA in vivo...
  74. pmc Phosphoregulators: protein kinases and protein phosphatases of mouse
    Alistair R R Forrest
    The Institute for Molecular Bioscience, Australia
    Genome Res 13:1443-54. 2003
    ..Finally, we comment on the complementary nature of cDNA and genome-based gene detection and the impact of the FANTOM2 transcriptome project...
  75. ncbi request reprint Phosphotyrosyl peptides and analogues as substrates and inhibitors of purple acid phosphatases
    Mohsen Valizadeh
    Department of Biochemistry, The University of Queensland, St Lucia, QLD 4072, Australia
    Arch Biochem Biophys 424:154-62. 2004
    ..These compounds are thus the most potent organic inhibitors yet reported for the purple acid phosphatases...
  76. ncbi request reprint The macrophage-inducible C-type lectin, mincle, is an essential component of the innate immune response to Candida albicans
    Christine A Wells
    National Centre for Adult Stem Cell Research, Eskitis Institute for Cell and Molecular Therapies, Griffith University, Brisbane, Queensland, Australia
    J Immunol 180:7404-13. 2008
    ..In addition, mice lacking Mincle showed a significantly increased susceptibility to systemic candidiasis. Thus, Mincle plays a novel and nonredundant role in the induction of inflammatory signaling in response to C. albicans infection...
  77. ncbi request reprint Inflammation suppressor genes: please switch out all the lights
    Christine A Wells
    Griffith University, Queensland, Australia
    J Leukoc Biol 78:9-13. 2005
    ....
  78. ncbi request reprint Genetics and genomics of osteoclast differentiation: integrating cell signaling pathways and gene networks
    Sudarshana M Sharma
    Human Cancer Genetics Program, Department of Molecular Virology, Immunology and Medical Genetics, Ohio State University, Columbus, OH 43210, USA
    Crit Rev Eukaryot Gene Expr 16:253-77. 2006
    ....
  79. ncbi request reprint Reduction of the in vitro pro-inflammatory response by macrophages to poly(3-hydroxybutyrate-co-3-hydroxyvalerate)
    Andy C K Wu
    Institute for Molecular Bioscience and Co operative Research Centre for Chronic Inflammatory Diseases, The University of Queensland, St Lucia 4072, Australia
    Biomaterials 27:4715-25. 2006
    ....
  80. pmc Macrophage-specific expression of human lysosomal acid lipase corrects inflammation and pathogenic phenotypes in lal-/- mice
    Cong Yan
    Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis, IN 46202, and Division of Human Genetics, Cincinnati Children s Hospital Medical Center, Cincinnati, OH 45229 3039, USA
    Am J Pathol 169:916-26. 2006
    ..These studies strongly support that neutral lipid metabolism in macrophages contributes to organ inflammation and pathogenesis...
  81. ncbi request reprint NF-IL6 and HSF1 have mutually antagonistic effects on transcription in monocytic cells
    Yue Xie
    Department of Radiation Oncology, Dana Farber Cancer Institute, Harvard Medical School, 44 Binney Street, Boston, Massachusetts 02115, USA
    Biochem Biophys Res Commun 291:1071-80. 2002
    ..Our studies suggest a strong mutual antagonism between the heat shock response and APR, which may influence the sensitivity and duration of inflammatory responses...
  82. pmc Transcript annotation in FANTOM3: mouse gene catalog based on physical cDNAs
    Norihiro Maeda
    Genome Science Laboratory, Discovery Research Institute, RIKEN Wako Institute, Wako, Japan
    PLoS Genet 2:e62. 2006
    ....
  83. ncbi request reprint Transcriptional network dynamics in macrophage activation
    Roland Nilsson
    Center for Genomics and Bioinformatics, Karolinska Institutet, Stockholm, Sweden
    Genomics 88:133-42. 2006
    ..We believe that our system approach presented here is applicable to understanding cellular differentiation in higher eukaryotes...
  84. ncbi request reprint The colony-stimulating factor 1 receptor is expressed on dendritic cells during differentiation and regulates their expansion
    Kelli P A MacDonald
    Bone Marrow Transplantation Laboratory, Queensland Institute of Medical Research, Queensland, Australia
    J Immunol 175:1399-405. 2005
    ..These data provide additional evidence that the majority of tissue DC is of myeloid origin during steady state and supports a close relationship between DC and macrophage biology in vivo...
  85. ncbi request reprint Transcription factor Tfec contributes to the IL-4-inducible expression of a small group of genes in mouse macrophages including the granulocyte colony-stimulating factor receptor
    Michael Rehli
    Department of Hematology and Oncology, University of Regensburg, Germany
    J Immunol 174:7111-22. 2005
    ..Our study also provides a general definition of the transcriptome in alternatively activated mouse macrophages and identifies a large number of novel genes characterizing this cell type...
  86. pmc Histone deacetylase inhibitors decrease Toll-like receptor-mediated activation of proinflammatory gene expression by impairing transcription factor recruitment
    Konrad A Bode
    Department of Medical Microbiology and Hygiene, University Heidelberg, Heidelberg, Germany
    Immunology 122:596-606. 2007
    ..Thus HDACs positively regulate the expression of a subset of cytokine genes by enabling transcription factor recruitment...
  87. pmc Targeting a complex transcriptome: the construction of the mouse full-length cDNA encyclopedia
    Piero Carninci
    Laboratory for Genome Exploration Research Group, RIKEN Genomic Sciences Center GSC, RIKEN Yokohama Institute, Suehiro cho, Tsurumi ku, Yokohama, Kanagawa 230 0045, Japan
    Genome Res 13:1273-89. 2003
    ..Clones accounting for about half of the predicted TUs await further sequencing. The continued high-discovery rate suggests that the task of transcriptome discovery is not yet complete...
  88. ncbi request reprint S100A8 chemotactic protein is abundantly increased, but only a minor contributor to LPS-induced, steroid resistant neutrophilic lung inflammation in vivo
    Steven Bozinovski
    Lung Disease Research Group, Departments of Pharmacology and Medicine, The University of Melbourne, Parkville 3010 VIC Australia
    J Proteome Res 4:136-45. 2005
    ....
  89. ncbi request reprint The transcriptional regulation of the Colony-Stimulating Factor 1 Receptor (csf1r) gene during hematopoiesis
    Constanze Bonifer
    University of Leeds, Leeds Institute of Molecular Medicine, St James s University Hospital, Section of Experimental Haematology, The Wellcome Trust Brenner Building, Leeds LS9 7TF, United Kingdom
    Front Biosci 13:549-60. 2008
    ..Aside from the direct relevance to hematopoiesis, studies of csf1r transcriptional regulation provide a model for understanding the molecular mechanisms that control mammalian cell fate...
  90. pmc Systematic expression profiling of the mouse transcriptome using RIKEN cDNA microarrays
    Hidemasa Bono
    Laboratory for Genome Exploration Research Group, RIKEN Genomic Sciences Center GSC, RIKEN Yokohama Institute, Suehiro cho, Tsurumi ku, Yokohama, Kanagawa 230 0045, Japan
    Genome Res 13:1318-23. 2003
    ..In addition, 1926 clones (70%) of 2768 clones that were categorized as "unknown EST," and 1969 (58%) clones of 3388 clones that were categorized as "unclassifiable" were also shown to be reproducibly expressed...
  91. ncbi request reprint Construction of representative transcript and protein sets of human, mouse, and rat as a platform for their transcriptome and proteome analysis
    Takeya Kasukawa
    Laboratory for Genome Exploration Research Group, RIKEN Genomic Sciences Center, RIKEN Yokohama Institute, Yokohama, Kanagawa 230 0045, Japan
    Genomics 84:913-21. 2004
    ..RTPSs of human, mouse, and rat have been produced by this method and used for validation. Their comprehensiveness and quality are discussed by comparison with other clustering approaches. The RTPSs are available at ...
  92. pmc Impact of alternative initiation, splicing, and termination on the diversity of the mRNA transcripts encoded by the mouse transcriptome
    Mihaela Zavolan
    Laboratory of Computational Genomics, The Rockefeller University, New York, New York 10021 6399, USA
    Genome Res 13:1290-300. 2003
    ....