Research Topics
Genomes and Genes
| David A HumeSummaryAffiliation: University of Queensland Country: Australia Publications
| Collaborators
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Detail Information
Publications
Alternate transcription of the Toll-like receptor signaling cascadeChristine A Wells
Eskitis Institute for Cell and Molecular Therapies, School of Biological and Biomedical Sciences, Griffith University, Brisbane 4111, Australia
Genome Biol 7:R10. 2006..Functional annotation of variant proteins was assessed in light of inflammatory signaling in mouse primary macrophages, and the expression of each variant transcript was assessed by splicing arrays...- Identification of a non-purple tartrate-resistant acid phosphatase: an evolutionary link to Ser/Thr protein phosphatases?Kieran S Hadler
School of Molecular and Microbial Sciences, The University of Queensland, St, Lucia, 4072, Australia
BMC Res Notes 1:78. 2008..Due to its role in bone resorption the 35 kDa TRAcP has become a promising target for the development of anti-osteoporotic chemotherapeutics... - Computational promoter analysis of mouse, rat and human antimicrobial peptide-coding genesManisha Brahmachary
Knowledge Extraction Laboratory, Institute for Infocomm Research, 21 Heng Mui Keng Terrace, Singapore 119613, Singapore
BMC Bioinformatics 7:S8. 2006..For many AMPcgs the promoter elements and transcription factors that control their tissue cell-specific expression have yet to be fully identified and characterized... 
The mononuclear phagocyte systemDavid A Hume
Institute for Molecular Bioscience, University of Queensland, Q4072, Australia
Curr Opin Immunol 18:49-53. 2006..The concept of the MPS may have partly outlived its usefulness...- Interferon-gamma: an overview of signals, mechanisms and functionsKate Schroder
Institute for Molecular Bioscience, University of Queensland, St Lucia, Brisbane 4072, Australia
J Leukoc Biol 75:163-89. 2004..In addition, integration of signaling and response with other cytokines and pathogen-associated molecular patterns, such as tumor necrosis factor-alpha, interleukin-4, type I IFNs, and lipopolysaccharide are discussed... - The mononuclear phagocyte system revisitedDavid A Hume
Institute for Molecular Bioscience, University of Queensland, Australia
J Leukoc Biol 72:621-7. 2002..We discuss the origins of macrophage heterogeneity and argue that macrophages and antigen-representing dendritic cells are closely related and part of the MPS... - Therapeutic targets in inflammatory diseaseDavid A Hume
Institute for Molecular Bioscience, University of Queensland, Brisbane, QLD 4072, Australia
Curr Med Chem 12:2925-9. 2005..It also highlights important limitations in the validation of inflammatory drug targets, and in the rate of discovery and development of new antiinflammatory drugs... 
Microphthalmia transcription factor regulates the expression of the novel osteoclast factor GPNMBVera M Ripoll
Institute for Molecular Biosciences, Co operative Research Centre for Chronic Inflammatory Diseases, The University of Queensland, St Lucia, QLD 4072, Australia
Gene 413:32-41. 2008..The inclusion of gpnmb in the MITF regulon suggests a role for GPNMB in mature osteoclast function...- Overview of the pipeline for structural and functional characterization of macrophage proteins at the university of queenslandWeining Meng
School of Molecular and Microbial Sciences, University of Queensland, Brisbane, Australia
Methods Mol Biol 426:577-87. 2008..Jointly, the data sheds light on the molecular and cellular functions of macrophage proteins... - Genetic control of the innate immune responseChristine A Wells
Institute for Molecular Biosciences, The University of Queensland, Brisbane, QLD, 4072, Australia
BMC Immunol 4:5. 2003.... - A conserved distal segment of the mouse CSF-1 receptor promoter is required for maximal expression of a reporter gene in macrophages and osteoclasts of transgenic miceDmitry A Ovchinnikov
Institute for Molecular Bioscience, University of Queensland, Australia
J Leukoc Biol 87:815-22. 2010..RT-PCR analyses of Csf1r mRNA revealed that mouse OCL use another promoter within this region, distinct from that used in placental trophoblasts, to generate an alternative 5'UTR... - PU.1 and ICSBP control constitutive and IFN-gamma-regulated Tlr9 gene expression in mouse macrophagesKate Schroder
Special Research Centre for Functional and Applied Genomics, Institute for Molecular Bioscience, University of Queensland, St Lucia, Brisbane 4072, Australia
J Leukoc Biol 81:1577-90. 2007..This study therefore characterizes the regulation of mouse Tlr9 expression and defines a molecular mechanism by which IFN-gamma amplifies mouse macrophage responses to CpG DNA... - LPS regulates a set of genes in primary murine macrophages by antagonising CSF-1 actionDavid P Sester
Cooperative Research Centre for Chronic Inflammatory Diseases, Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland 4072, Australia
Immunobiology 210:97-107. 2005..This finding also provides a biological relevance to the well-documented ability of macrophage activators to down-modulate surface expression of the CSF-1R... - Structural basis for recruitment of tandem hotdog domains in acyl-CoA thioesterase 7 and its role in inflammationJade K Forwood
School of Molecular and Microbial Sciences, University of Queensland, Brisbane, Queensland 4072, Australia
Proc Natl Acad Sci U S A 104:10382-7. 2007..Together, the results link the molecular and cellular functions of Acot7 and identify the enzyme as a candidate drug target in inflammatory disease... - Expression of Gal4-dependent transgenes in cells of the mononuclear phagocyte system labeled with enhanced cyan fluorescent protein using Csf1r-Gal4VP16/UAS-ECFP double-transgenic miceDmitry A Ovchinnikov
Institute for Molecular Bioscience, Bldg 80, University of Queensland, Brisbane, Queensland 4072, Australia
J Leukoc Biol 83:430-3. 2008..The new mouse line provides a useful tool for overexpression of transgenes in cells of the myeloid lineage, while simultaneously labeling them by ECFP expression... - Continued discovery of transcriptional units expressed in cells of the mouse mononuclear phagocyte lineageChristine A Wells
Institute for Molecular Bioscience and ARC Special Research Centre for Functional and Applied Genomics, Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland 4072, Australia
Genome Res 13:1360-5. 2003.... - CpG DNA activates survival in murine macrophages through TLR9 and the phosphatidylinositol 3-kinase-Akt pathwayDavid P Sester
Cooperative Research Centre for Chronic Inflammatory Diseases and Special Research Centre for Functional and Applied Genomics, Institute for Molecular Bioscience, University of Queensland, St Lucia, Brisbane, QLD 4072, Australia
J Immunol 177:4473-80. 2006..Therefore, CpG DNA activates macrophage survival via TLR9 and the PI3K-Akt pathway and independently of DNA-PK and MEK-ERK... - The JNK are important for development and survival of macrophagesS Roy Himes
Cooperative Research Centre for Chronic Inflammatory Disease, Institute for Molecular Biosciences, University of Queensland, Brisbane, Australia
J Immunol 176:2219-28. 2006..This pattern of expression may underlie the novel role of JNK in macrophages... - LPS regulates proinflammatory gene expression in macrophages by altering histone deacetylase expressionHnin Thanda Aung
Cooperative Research Centre for Chronic Inflammatory Diseases, Institute for Molecular Bioscience, University of Queensland, Australia
FASEB J 20:1315-27. 2006..1 degradation. Hence, HDACs act as potent and selective negative regulators of proinflammatory gene expression and act to prevent excessive inflammatory responses in macrophages... - The Ewing sarcoma protein (EWS) binds directly to the proximal elements of the macrophage-specific promoter of the CSF-1 receptor (csf1r) geneDavid A Hume
Australian Research Council Special Research Centre for Functional and Applied Genomics and the Cooperative Research Centre for Chronic Inflammatory Diseases, University of Queensland, Brisbane, Queensland, Australia
J Immunol 180:6733-42. 2008..Transfection assays suggest that EWS does not act as a conventional transcriptional activator or repressor. We hypothesize that EWS contributes to start site recognition in TATA-less mammalian promoters... - Gpnmb is induced in macrophages by IFN-gamma and lipopolysaccharide and acts as a feedback regulator of proinflammatory responsesVera M Ripoll
Cooperative Research Centre for Chronic Inflammatory Diseases and Special Research Centre for Functional and Applied Genomics, Institute for Molecular Bioscience, University of Queensland, Queensland, Australia
J Immunol 178:6557-66. 2007..Thus, GPNMB acts as a negative regulator of macrophage inflammatory responses... - Differential effects of CpG DNA on IFN-beta induction and STAT1 activation in murine macrophages versus dendritic cells: alternatively activated STAT1 negatively regulates TLR signaling in macrophagesKate Schroder
Cooperative Research Centre for Chronic Inflammatory Diseases and Special Research Centre for Functional and Applied Genomics, Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland, Australia
J Immunol 179:3495-503. 2007..The differential activation of Ifn-beta and STAT1 by CpG DNA in mouse macrophages vs dendritic cells provides a likely mechanism for their divergent roles in priming the adaptive immune response... - CD148/DEP-1 association with areas of cytoskeletal organisation in macrophagesRicha K Dave
Institute for Molecular Bioscience, The University of Queensland, St Lucia, QLD, Australia
Exp Cell Res 315:1734-44. 2009..There were no detectable effects on the CSF-1 receptor-akt signalling pathway. These results are consistent with the hypothesis that CD148 is a regulator of macrophage activity... - Differential effects of selective HDAC inhibitors on macrophage inflammatory responses to the Toll-like receptor 4 agonist LPSMaria A Halili
The University of Queensland, Institute for Molecular Bioscience, S Lucia, Brisbane, Queensland, 4072, Australia
J Leukoc Biol 87:1103-14. 2010..Thus, 17a provides a tool to identify individual HDACs with proinflammatory properties... - Colony-stimulating factor-1 (CSF-1) delivers a proatherogenic signal to human macrophagesKatharine M Irvine
The University of Queensland, Institute for Molecular Bioscience, Brisbane, Queensland, Australia
J Leukoc Biol 85:278-88. 2009.... - Systematic characterization of the zinc-finger-containing proteins in the mouse transcriptomeTimothy Ravasi
Institute for Molecular Bioscience, Brisbane, Australia
Genome Res 13:1430-42. 2003.... - A medium or high throughput protein refolding assayNathan P Cowieson
Institute for Molecular Bioscience, University of Queensland, Brisbane, Australia
Methods Mol Biol 426:269-75. 2008..Two formats of the assay are described, a manual assay that is useful for screening small numbers of targets, and an automated implementation that is useful for large numbers of targets... - Mouse neutrophilic granulocytes express mRNA encoding the macrophage colony-stimulating factor receptor (CSF-1R) as well as many other macrophage-specific transcripts and can transdifferentiate into macrophages in vitro in response to CSF-1R Tedjo Sasmono
CRC for Chronic Inflammatory Diseases and ARC Special Research Centre for Functional and Applied Genomics, Institute for Molecular Bioscience, The University of Queensland, St Lucia, Australia
J Leukoc Biol 82:111-23. 2007..In keeping with this hypothesis, we showed that purified Ly-6G-positive granulocytes express CSF-1R after overnight culture and can subsequently differentiate to form F4/80-positive macrophages in response to CSF-1... - Osteal tissue macrophages are intercalated throughout human and mouse bone lining tissues and regulate osteoblast function in vitro and in vivoMing K Chang
Institute for Molecular Bioscience, Cooperative Research Centre for Chronic Inflammatory Diseases, University of Queensland, Brisbane, Queensland, Australia
J Immunol 181:1232-44. 2008..These observations implicate OsteoMacs, in addition to osteoclasts and osteoblasts, as principal participants in bone dynamics... - Human tartrate-resistant acid phosphatase becomes an effective ATPase upon proteolytic activationNatasa Mitic
School of Molecular and Microbial Sciences, The University of Queensland, St Lucia, Australia
Arch Biochem Biophys 439:154-64. 2005..Notably, at low pH this residue may act as a proton donor for the leaving group. In this respect the mechanism of cleaved TRAcP resembles that of sweet potato purple acid phosphatase... - Conservation and divergence in Toll-like receptor 4-regulated gene expression in primary human versus mouse macrophagesKate Schroder
Institute for Molecular Bioscience, University of Queensland, Brisbane 4072, Australia
Proc Natl Acad Sci U S A 109:E944-53. 2012..Thus, the genes controlled by complex, highly conserved promoters that facilitate dynamic regulation are also the most susceptible to evolutionary change... - A general target selection method for crystallographic proteomicsGautier Robin
Institute for Molecular Bioscience, University of Queensland, Brisbane, Australia
Methods Mol Biol 426:27-35. 2008..This novel procedure was designed to maximize selection efficiency, and its prevailing criteria categories make it suitable for a broad range of structural proteomics projects... - Protein structure determination using a combination of cross-linking, mass spectrometry, and molecular modelingDmitri Mouradov
School of Molecular and Microbial Sciences, University of Queensland, Brisbane, Australia
Methods Mol Biol 426:459-74. 2008.... - Macrophage activation and differentiation signals regulate schlafen-4 gene expression: evidence for Schlafen-4 as a modulator of myelopoiesisWendy J van Zuylen
The University of Queensland, Institute for Molecular Bioscience, Queensland, Australia
PLoS ONE 6:e15723. 2011..Little is known of their function, but previous studies suggest roles in immune cell development. In this report, we assessed Slfn regulation and function in macrophages, which are key cellular regulators of innate immunity... - A CSF-1 receptor kinase inhibitor targets effector functions and inhibits pro-inflammatory cytokine production from murine macrophage populationsKatharine M Irvine
Cooperative Research Centre for Chronic Inflammatory Diseases and Special Research Centre for Functional and Applied Genomics, Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland, Australia
FASEB J 20:1921-3. 2006..CSF-1R inhibitors such as CYC10268 provide a powerful tool to dissect the role of the CSF-1/CSF-1R signaling system in a range of biological systems and have potential for a number of therapeutic applications... - Differences in macrophage activation by bacterial DNA and CpG-containing oligonucleotidesTara L Roberts
Institute for Molecular Bioscience, University of Queensland, Brisbane, Australia
J Immunol 175:3569-76. 2005..The length dependence of the CpG ODN response was found to correlate with the presence in macrophages of a length-dependent uptake process for DNA. This transport system was absent from B cells and fibroblasts... - Incorporating a TEV cleavage site reduces the solubility of nine recombinant mouse proteinsMareike Kurz
Institute for Molecular Bioscience, University of Queensland, Brisbane, QLD 4072, Australia
Protein Expr Purif 50:68-73. 2006..Our work suggests that one needs to be very careful when making modifications to expression vectors and combining different affinity and fusion tags and cleavage sites... - Transcriptional regulatory networks in macrophagesDavid A Hume
ARC Special Research Centre for Functional and Applied Genomics and CRC for Chronic Inflammatory Diseases, Institute for Molecular Bioscience, University of Queensland, Australia
Novartis Found Symp 281:2-18; discussion 18-24, 50-3, 208-9. 2007.... - An antibody against the colony-stimulating factor 1 receptor depletes the resident subset of monocytes and tissue- and tumor-associated macrophages but does not inhibit inflammationKelli P A MacDonald
Queensland Institute of Medical Research, Brisbane, Australia
Blood 116:3955-63. 2010..The data indicate that CSF1R signaling is required only for the maturation and replacement of resident-type monocytes and tissue macrophages, and is not required for monocyte production or inflammatory function... - HIN-200 proteins regulate caspase activation in response to foreign cytoplasmic DNATara L Roberts
The University of Queensland, Institute for Molecular Bioscience, QLD 4072, Australia
Science 323:1057-60. 2009..This work indicates that HIN-200 proteins can act as pattern recognition receptors mediating responses to cytoplasmic dsDNA... - Experimental validation of the regulated expression of large numbers of non-coding RNAs from the mouse genomeTimothy Ravasi
ARC Special Research Centre for Functional and Applied Genomics, Institute for Molecular Bioscience, University of Queensland, Brisbane QLD 4072, Australia
Genome Res 16:11-9. 2006..Taken together, the data provide strong support for the conclusion that ncRNAs are an important, regulated component of the mammalian transcriptome... - TLR9-independent effects of inhibitory oligonucleotides on macrophage responses to S. typhimuriumAngela Trieu
The University of Queensland, Institute for Molecular Bioscience, Queensland, Australia
Immunol Cell Biol 87:218-25. 2009.... - Cutting edge: species-specific TLR9-mediated recognition of CpG and non-CpG phosphorothioate-modified oligonucleotidesTara L Roberts
Institute for Molecular Bioscience, Cooperative Research Centre for Chronic Inflammatory Diseases, University of Queensland, Brisbane, Australia
J Immunol 174:605-8. 2005..Thus, TLR9 may be responsible for mediating many published CpG-independent responses to PS-ODN... - A rescue strategy for multimapping short sequence tags refines surveys of transcriptional activity by CAGEGeoffrey J Faulkner
The Institute for Molecular Bioscience, University of Queensland, Brisbane, QLD 4072, Australia
Genomics 91:281-8. 2008..These results suggest that the multimap tags produced by high-throughput, short sequence tag-based approaches can be rescued to augment greatly the transcriptome coverage provided by single-map tags alone... - Characterisation and trophic functions of murine embryonic macrophages based upon the use of a Csf1r-EGFP transgene reporterFiona Rae
Institute for Molecular Bioscience and ARC Special Research Centre for Functional and Applied Genomics, University of Queensland, Brisbane, Queensland 4072, Australia
Dev Biol 308:232-46. 2007..These findings suggest a trophic role of macrophages in embryonic kidney development, which may continue to play a similar role in postnatal repair... - An automatable screen for the rapid identification of proteins amenable to refoldingNathan P Cowieson
Institute for Molecular Bioscience and ARC Special Research Centre for Functional and Applied Genomics, University of Queensland, Brisbane, Queensland, Australia
Proteomics 6:1750-7. 2006..The efficacy of the screen is demonstrated on small-scale expression samples for 15 proteins. Refolding is then validated by large-scale expressions using SEC and circular dichroism... - CSF-1 as a regulator of macrophage activation and immune responsesMatthew J Sweet
CRC for Chronic Inflammatory Diseases, Institute for Molecular Bioscience and Department of Microbiology Parasitology, University of Queensland, QLD 4072, Australia
Arch Immunol Ther Exp (Warsz) 51:169-77. 2003..This review will summarize our current understanding of the effects of CSF-1 on the activation state of mature macrophages and its role in regulating immune responses... - Identification and analysis of chromodomain-containing proteins encoded in the mouse transcriptomeKhairina Tajul-Arifin
ARC Special Research Centre for Functional and Applied Genomics, Institute for Molecular Bioscience, University of Queensland, St.Lucia, Queensland 4072, Australia
Genome Res 13:1416-29. 2003..The likely functions of these proteins are discussed in relation to the known functions of other chromodomain-containing proteins within the same family... - An inflammatory role for the mammalian carboxypeptidase inhibitor latexin: relationship to cystatins and the tumor suppressor TIG1Anna Aagaard
Institute for Molecular Bioscience, University of Queensland, Brisbane, QLD 4072, Australia
Structure 13:309-17. 2005..The structure of the tumor suppressor protein TIG1 was modeled, revealing its putative membrane binding surface... - Generation of diversity in the innate immune system: macrophage heterogeneity arises from gene-autonomous transcriptional probability of individual inducible genesTimothy Ravasi
Institute for Molecular Bioscience, and Cooperative Research Center for Chronic Inflammatory Diseases, University of Queensland, Brisbane, Queensland 4072, Australia
J Immunol 168:44-50. 2002..The results indicate that each LPS-inducible gene has its own inherent probability of activation in response to LPS... - Probing the S100 protein family through genomic and functional analysisTimothy Ravasi
SRC for Functional and Applied Genomics, University of Queensland, Brisbabe, OLD, Australia
Genomics 84:10-22. 2004..Evolution of the S100 proteins appears to have occurred in a modular fashion, also seen in other protein families such as the C2H2-type zinc-finger family... - Focusing in on structural genomics: the University of Queensland structural biology pipelineMunish Puri
Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland, Australia
Biomol Eng 23:281-9. 2006..The structures from this pipeline will provide insights into the function of previously uncharacterized macrophage proteins and could lead to the validation of new drug targets for chronic obstructive pulmonary disease and arthritis... - Selective induction of the Notch ligand Jagged-1 in macrophages by soluble egg antigen from Schistosoma mansoni involves ERK signallingFelicia Goh
Institute for Molecular Bioscience, University of Queensland, QLD, Australia
Immunology 127:326-37. 2009..Taken together, our data suggest that Jagged-1 is an ERK-dependent target of TLR signalling that has a macrophage-specific function in the response to SEA... - The Runx1 transcription factor controls CSF-1-dependent and -independent growth and survival of macrophagesStewart R Himes
CRC for Chronic Inflammatory Disease, Institute for Molecular Biosciences, Queensland Biosciences Precinct, Bldg 80, Services Rd, University of Queensland, Brisbane, Queensland 4072, Australia
Oncogene 24:5278-86. 2005..These findings suggest that Runx1 regulates growth and survival of myeloid cells and provide a novel insight into the role of Runx family gene translocations in leukemogenesis... - Multiple tissue-specific promoters control expression of the murine tartrate-resistant acid phosphatase geneNicole C Walsh
Institute for Molecular Bioscience and School of Molecular and Microbial Sciences, University of Queensland, Brisbane, Australia
Gene 307:111-23. 2003..The distinct expression patterns for each of the TRAP 5'-UTRs suggest that TRAP mRNA expression is regulated by the use of four alternative tissue- and cell-restricted promoters... - Identification of disulfide-containing chemical cross-links in proteins using MALDI-TOF/TOF-mass spectrometryGordon J King
Cooperative Research Centre for Chronic Inflammatory Diseases, Institute for Molecular Bioscience, University of Queensland, St Lucia Brisbane
Anal Chem 80:5036-43. 2008..The methodology we report is robust and amenable to automation, and permits the analysis of native cystines along with those introduced by disulfide-containing cross-linkers... - Syntaxin 6 and Vti1b form a novel SNARE complex, which is up-regulated in activated macrophages to facilitate exocytosis of tumor necrosis Factor-alphaRachael Z Murray
Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland, Australia 4072
J Biol Chem 280:10478-83. 2005..This study shows how macrophages have uniquely adapted a novel Golgi-associated SNARE complex to accommodate their requirement for increased cytokine secretion... - The expression of Clcn7 and Ostm1 in osteoclasts is coregulated by microphthalmia transcription factorNicholas A Meadows
Institute for Molecular Biosciences, The University of Queensland, St Lucia, Queensland 4072, Australia
J Biol Chem 282:1891-904. 2007.... - Modelling the structure of latexin-carboxypeptidase A complex based on chemical cross-linking and molecular dockingDmitri Mouradov
School of Molecular and Microbial Sciences, Institute for Molecular Bioscience, Cooperative Research Centre for Chronic Inflammatory Diseases, Department of Mathematics, The University of Queensland, Brisbane, Queensland 4072, Australia
Protein Eng Des Sel 19:9-16. 2006..m.s. deviation of Calpha atoms of the crystal structure. The study demonstrates that cross-linking in combination with mass spectrometry can lead to efficient and accurate structural modelling of protein complexes... - Pilot studies on the parallel production of soluble mouse proteins in a bacterial expression systemNathan P Cowieson
Institute for Molecular Bioscience, Australia
J Struct Funct Genomics 6:13-20. 2005..This success rate compares favourably with other protein screening projects, particularly for eukaryotic proteins, and could be further improved by modifications at the cloning step... - The immunostimulatory activity of phosphorothioate CpG oligonucleotides is affected by distal sequence changesTara L Roberts
The University of Queensland, Institute for Molecular Bioscience, QLD 4072, Australia
Mol Immunol 48:1027-34. 2011..The reduction in immunostimulatory activity of PS-ODN was associated with a delay in the activation of MAP kinases... - Modification of recombinatorial cloning for small affinity tag fusion protein construct generationPawel Listwan
School of Molecular and Microbial Sciences, University of Queensland, Brisbane, Queensland 4072, Australia
Anal Biochem 346:327-9. 2005 - The regulated retrotransposon transcriptome of mammalian cellsGeoffrey J Faulkner
Institute for Molecular Bioscience, University of Queensland, Australia
Nat Genet 41:563-71. 2009..We conclude that retrotransposon transcription has a key influence upon the transcriptional output of the mammalian genome... - Beta-arrestin 2 is required for complement C1q expression in macrophages and constrains factor-independent survivalJane E Lattin
The University of Queensland, Institute for Molecular Bioscience, QLD 4072, Australia
Mol Immunol 47:340-7. 2009..Given that loss of C1q function is strongly associated with the development of systemic lupus erythematosus, ARRB2 may act to limit the development of autoimmune disease... - A macrophage colony-stimulating factor receptor-green fluorescent protein transgene is expressed throughout the mononuclear phagocyte system of the mouseR Tedjo Sasmono
Institute for Molecular Bioscience and ARC Special Research Centre for Functional and Applied Genomics, University of Queensland, Brisbane, Australia
Blood 101:1155-63. 2003..We have therefore defined the elements required to generate myeloid- and trophoblast-specific transgenes as well as a model system for the study of mononuclear phagocyte development and function... - Tiny RNAs associated with transcription start sites in animalsRyan J Taft
Institute for Molecular Bioscience, The University of Queensland, St Lucia, Australia
Nat Genet 41:572-8. 2009..tiRNAs are generally, although not exclusively, associated with highly expressed transcripts and sites of RNA polymerase II binding. We suggest that tiRNAs may be a general feature of transcription in metazoa and possibly all eukaryotes... - Osteal macrophages: a new twist on coupling during bone dynamicsAllison R Pettit
The University of Queensland, Institute for Molecular Bioscience, Cooperative Research Centre for Chronic Inflammatory Diseases, Brisbane, QLD 4072, Australia
Bone 43:976-82. 2008..This review discusses the wider impact of macrophages in bone homeostasis and disease and proposes novel roles for OsteoMacs in bone modelling and remodelling... - G-protein-coupled receptor expression, function, and signaling in macrophagesJane Lattin
Cooperative Research Centre for Chronic Inflammatory Diseases, University of Queensland, St Lucia, Brisbane, QLD 4072, Australia
J Leukoc Biol 82:16-32. 2007..In particular, we examine the crosstalk between GPCR and TLR signaling pathways and highlight GPCR signaling molecules which are likely to have uncharacterized functions in this cell lineage... - The mouse secretome: functional classification of the proteins secreted into the extracellular environmentSean M Grimmond
Institute for Molecular Bioscience and ARC Special Research Centre for Functional and Applied Genomics, University of Queensland, St Lucia 4072, Australia
Genome Res 13:1350-9. 2003..To highlight the utility of this information, we discuss the CUB domain-containing protein family... - Colony-stimulating factor-1 suppresses responses to CpG DNA and expression of toll-like receptor 9 but enhances responses to lipopolysaccharide in murine macrophagesMatthew J Sweet
Department of Immunology and Bacteriology, University of Glasgow, Glasgow, United Kingdom
J Immunol 168:392-9. 2002..Hence, CSF-1 may regulate host responses to pathogens through modulation of TLR expression. Furthermore, these results suggest that CSF-1 and CSF-1R antagonists may enhance the efficacy of CpG DNA in vivo... - The molecular basis for the lack of immunostimulatory activity of vertebrate DNAKatryn J Stacey
Cooperative Research Center for Chronic Inflammatory Diseases, Institute for Molecular Bioscience, School of Molecular and Microbial Sciences, University of Queensland, Brisbane, Australia
J Immunol 170:3614-20. 2003..The immunostimulatory activity of DNA is determined by the frequency of unmethylated stimulatory sequences within an individual DNA strand and the ratio of stimulatory to inhibitory sequences... - Signal integration between IFNgamma and TLR signalling pathways in macrophagesKate Schroder
Institute for Molecular Bioscience, University of Queensland, QLD Bioscience Precinct, Brisbane, QLD 4072, Australia
Immunobiology 211:511-24. 2006..Synergy occurs at multiple levels, ranging from signal recognition to convergence of signals at the promoters of target genes. In particular, the cross-talk between the IFNgamma, and LPS and CpG DNA signalling pathways is discussed... - Phosphoregulators: protein kinases and protein phosphatases of mouseAlistair R R Forrest
The Institute for Molecular Bioscience, Australia
Genome Res 13:1443-54. 2003..Finally, we comment on the complementary nature of cDNA and genome-based gene detection and the impact of the FANTOM2 transcriptome project... - Phosphotyrosyl peptides and analogues as substrates and inhibitors of purple acid phosphatasesMohsen Valizadeh
Department of Biochemistry, The University of Queensland, St. Lucia, QLD 4072, Australia
Arch Biochem Biophys 424:154-62. 2004..These compounds are thus the most potent organic inhibitors yet reported for the purple acid phosphatases... - The macrophage-inducible C-type lectin, mincle, is an essential component of the innate immune response to Candida albicansChristine A Wells
National Centre for Adult Stem Cell Research, Eskitis Institute for Cell and Molecular Therapies, Griffith University, Brisbane, Queensland, Australia
J Immunol 180:7404-13. 2008..In addition, mice lacking Mincle showed a significantly increased susceptibility to systemic candidiasis. Thus, Mincle plays a novel and nonredundant role in the induction of inflammatory signaling in response to C. albicans infection... - Genetics and genomics of osteoclast differentiation: integrating cell signaling pathways and gene networksSudarshana M Sharma
Human Cancer Genetics Program, Department of Molecular Virology, Immunology and Medical Genetics, Ohio State University, Columbus, OH 43210, USA
Crit Rev Eukaryot Gene Expr 16:253-77. 2006.... - Reduction of the in vitro pro-inflammatory response by macrophages to poly(3-hydroxybutyrate-co-3-hydroxyvalerate)Andy C K Wu
Institute for Molecular Bioscience and Co-operative Research Centre for Chronic Inflammatory Diseases, The University of Queensland, St Lucia 4072, Australia
Biomaterials 27:4715-25. 2006.... - NF-IL6 and HSF1 have mutually antagonistic effects on transcription in monocytic cellsYue Xie
Department of Radiation Oncology, Dana Farber Cancer Institute, Harvard Medical School, 44 Binney Street, Boston, Massachusetts 02115, USA
Biochem Biophys Res Commun 291:1071-80. 2002..Our studies suggest a strong mutual antagonism between the heat shock response and APR, which may influence the sensitivity and duration of inflammatory responses... - Transcriptional network dynamics in macrophage activationRoland Nilsson
Center for Genomics and Bioinformatics, Karolinska Institutet, Stockholm, Sweden
Genomics 88:133-42. 2006..We believe that our system approach presented here is applicable to understanding cellular differentiation in higher eukaryotes... - Construction of representative transcript and protein sets of human, mouse, and rat as a platform for their transcriptome and proteome analysisTakeya Kasukawa
Laboratory for Genome Exploration Research Group, RIKEN Genomic Sciences Center, RIKEN Yokohama Institute, Yokohama, Kanagawa 230 0045, Japan
Genomics 84:913-21. 2004..RTPSs of human, mouse, and rat have been produced by this method and used for validation. Their comprehensiveness and quality are discussed by comparison with other clustering approaches. The RTPSs are available at ... - Transcript annotation in FANTOM3: mouse gene catalog based on physical cDNAsNorihiro Maeda
Genome Science Laboratory, Discovery Research Institute, RIKEN Wako Institute, Wako, Japan
PLoS Genet 2:e62. 2006.... - Macrophage-specific expression of human lysosomal acid lipase corrects inflammation and pathogenic phenotypes in lal-/- miceCong Yan
Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis, IN 46202, and Division of Human Genetics, Cincinnati Children s Hospital Medical Center, Cincinnati, OH 45229 3039, USA
Am J Pathol 169:916-26. 2006..These studies strongly support that neutral lipid metabolism in macrophages contributes to organ inflammation and pathogenesis... - The colony-stimulating factor 1 receptor is expressed on dendritic cells during differentiation and regulates their expansionKelli P A MacDonald
Bone Marrow Transplantation Laboratory, Queensland Institute of Medical Research, Queensland, Australia
J Immunol 175:1399-405. 2005..These data provide additional evidence that the majority of tissue DC is of myeloid origin during steady state and supports a close relationship between DC and macrophage biology in vivo... - Histone deacetylase inhibitors decrease Toll-like receptor-mediated activation of proinflammatory gene expression by impairing transcription factor recruitmentKonrad A Bode
Department of Medical Microbiology and Hygiene, University Heidelberg, Heidelberg, Germany
Immunology 122:596-606. 2007..Thus HDACs positively regulate the expression of a subset of cytokine genes by enabling transcription factor recruitment... - The transcriptional regulation of the Colony-Stimulating Factor 1 Receptor (csf1r) gene during hematopoiesisConstanze Bonifer
University of Leeds, Leeds Institute of Molecular Medicine, St James s University Hospital, Section of Experimental Haematology, The Wellcome Trust Brenner Building, Leeds LS9 7TF, United Kingdom
Front Biosci 13:549-60. 2008..Aside from the direct relevance to hematopoiesis, studies of csf1r transcriptional regulation provide a model for understanding the molecular mechanisms that control mammalian cell fate... - Transcription factor Tfec contributes to the IL-4-inducible expression of a small group of genes in mouse macrophages including the granulocyte colony-stimulating factor receptorMichael Rehli
Department of Hematology and Oncology, University of Regensburg, Germany
J Immunol 174:7111-22. 2005..Our study also provides a general definition of the transcriptome in alternatively activated mouse macrophages and identifies a large number of novel genes characterizing this cell type... - Inflammation suppressor genes: please switch out all the lightsChristine A Wells
Griffith University, Queensland, Australia
J Leukoc Biol 78:9-13. 2005.... - Impact of alternative initiation, splicing, and termination on the diversity of the mRNA transcripts encoded by the mouse transcriptomeMihaela Zavolan
Laboratory of Computational Genomics, The Rockefeller University, New York, New York 10021 6399, USA
Genome Res 13:1290-300. 2003.... - S100A8 chemotactic protein is abundantly increased, but only a minor contributor to LPS-induced, steroid resistant neutrophilic lung inflammation in vivoSteven Bozinovski
Lung Disease Research Group, Departments of Pharmacology and Medicine, The University of Melbourne, Parkville 3010 VIC Australia
J Proteome Res 4:136-45. 2005.... - Systematic expression profiling of the mouse transcriptome using RIKEN cDNA microarraysHidemasa Bono
Laboratory for Genome Exploration Research Group, RIKEN Genomic Sciences Center (GSC, RIKEN Yokohama Institute, Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan
Genome Res 13:1318-23. 2003..In addition, 1926 clones (70%) of 2768 clones that were categorized as "unknown EST," and 1969 (58%) clones of 3388 clones that were categorized as "unclassifiable" were also shown to be reproducibly expressed... - Targeting a complex transcriptome: the construction of the mouse full-length cDNA encyclopediaPiero Carninci
Laboratory for Genome Exploration Research Group, RIKEN Genomic Sciences Center (GSC, RIKEN Yokohama Institute, Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan
Genome Res 13:1273-89. 2003..Clones accounting for about half of the predicted TUs await further sequencing. The continued high-discovery rate suggests that the task of transcriptome discovery is not yet complete...