Maria Hrmova

Summary

Affiliation: University of Adelaide
Country: Australia

Publications

  1. ncbi request reprint A Barley xyloglucan xyloglucosyl transferase covalently links xyloglucan, cellulosic substrates, and (1,3;1,4)-beta-D-glucans
    Maria Hrmova
    School of Agriculture, Food and Wine, and Australian Centre for Plant Functional Genomics, University of Adelaide, Waite Campus, Glen Osmond, South Australia 5064, Australia
    J Biol Chem 282:12951-62. 2007
  2. pmc Plant High-Affinity Potassium (HKT) Transporters Involved in Salinity Tolerance: Structural Insights to Probe Differences in Ion Selectivity
    Shane Waters
    Australian Centre for Plant Functional Genomics and Waite Research Institute, University of Adelaide, Glen Osmond, South Australia 5064, Australia
    Int J Mol Sci 14:7660-80. 2013
  3. doi request reprint Substrate specificity and catalytic mechanism of a xyloglucan xyloglucosyl transferase HvXET6 from barley (Hordeum vulgare L.)
    Maria Hrmova
    School of Agriculture, Food and Wine, Australian Centre for Plant Functional Genomics, University of Adelaide, Australia
    FEBS J 276:437-56. 2009
  4. ncbi request reprint High-yield production, refolding and a molecular modelling of the catalytic module of (1,3)-beta-D-glucan (curdlan) synthase from Agrobacterium sp
    Maria Hrmova
    Australian Centre for Plant Functional Genomics, School of Agriculture, Food and Wine, University of Adelaide, Waite Campus, Glen Osmond, SA, 5064, Australia
    Glycoconj J 27:461-76. 2010
  5. ncbi request reprint Dissecting the catalytic mechanism of a plant beta-D-glucan glucohydrolase through structural biology using inhibitors and substrate analogues
    Maria Hrmova
    Australian Centre for Plant Functional Genomics, School of Agriculture, Food and Wine, University of Adelaide, Waite Campus, Glen Osmond, SA 5064, Australia
    Carbohydr Res 342:1613-23. 2007
  6. pmc Hydrolysis of (1,4)-beta-D-mannans in barley (Hordeum vulgare L.) is mediated by the concerted action of (1,4)-beta-D-mannan endohydrolase and beta-D-mannosidase
    Maria Hrmova
    School of Agriculture, Food and Wine, University of Adelaide and Australian Centre for Plant Functional Genomics, Waite Campus, Glen Osmond, SA 5064, Australia
    Biochem J 399:77-90. 2006
  7. ncbi request reprint Structural rationale for low-nanomolar binding of transition state mimics to a family GH3 beta-D-glucan glucohydrolase from barley
    Maria Hrmova
    School of Agriculture and Wine and Australian Centre for Plant Functional Genomics, University of Adelaide, Waite Campus, Glen Osmond, SA 5064, Australia
    Biochemistry 44:16529-39. 2005
  8. ncbi request reprint Three-dimensional structure of the barley beta-D-glucan glucohydrolase in complex with a transition state mimic
    Maria Hrmova
    Faculty of Sciences, School of Agriculture and Wine, University of Adelaide, Waite Campus, Glen Osmond, SA 5064, Australia
    J Biol Chem 279:4970-80. 2004
  9. pmc Structural basis for broad substrate specificity in higher plant beta-D-glucan glucohydrolases
    Maria Hrmova
    Department of Plant Science, University of Adelaide, Waite Campus, Glen Osmond, South Australia 5064, Australia
    Plant Cell 14:1033-52. 2002
  10. pmc The genetics, transcriptional profiles, and catalytic properties of UDP-alpha-D-xylose 4-epimerases from barley
    Qisen Zhang
    Australian Centre for Plant Functional Genomics, School of Agriculture, Food, and Wine, University of Adelaide, Waite Campus, Glen Osmond, South Australia 5064, Australia
    Plant Physiol 153:555-68. 2010

Collaborators

Detail Information

Publications35

  1. ncbi request reprint A Barley xyloglucan xyloglucosyl transferase covalently links xyloglucan, cellulosic substrates, and (1,3;1,4)-beta-D-glucans
    Maria Hrmova
    School of Agriculture, Food and Wine, and Australian Centre for Plant Functional Genomics, University of Adelaide, Waite Campus, Glen Osmond, South Australia 5064, Australia
    J Biol Chem 282:12951-62. 2007
    ..These findings raise the possibility that xyloglucan endo-transglucosylases could link different polysaccharides in vivo and hence influence cell wall strength, flexibility, and porosity...
  2. pmc Plant High-Affinity Potassium (HKT) Transporters Involved in Salinity Tolerance: Structural Insights to Probe Differences in Ion Selectivity
    Shane Waters
    Australian Centre for Plant Functional Genomics and Waite Research Institute, University of Adelaide, Glen Osmond, South Australia 5064, Australia
    Int J Mol Sci 14:7660-80. 2013
    ....
  3. doi request reprint Substrate specificity and catalytic mechanism of a xyloglucan xyloglucosyl transferase HvXET6 from barley (Hordeum vulgare L.)
    Maria Hrmova
    School of Agriculture, Food and Wine, Australian Centre for Plant Functional Genomics, University of Adelaide, Australia
    FEBS J 276:437-56. 2009
    ..The two HvXETs followed a ping-pong (Bi, Bi) rather than a sequential reaction mechanism...
  4. ncbi request reprint High-yield production, refolding and a molecular modelling of the catalytic module of (1,3)-beta-D-glucan (curdlan) synthase from Agrobacterium sp
    Maria Hrmova
    Australian Centre for Plant Functional Genomics, School of Agriculture, Food and Wine, University of Adelaide, Waite Campus, Glen Osmond, SA, 5064, Australia
    Glycoconj J 27:461-76. 2010
    ..A three-dimensional model of cm-CrdS predicted the juxtaposition of highly conserved aspartates D156, D208, D210 and D304, and the QRTRW motif, which are likely to play roles in donor and acceptor substrate binding and catalysis...
  5. ncbi request reprint Dissecting the catalytic mechanism of a plant beta-D-glucan glucohydrolase through structural biology using inhibitors and substrate analogues
    Maria Hrmova
    Australian Centre for Plant Functional Genomics, School of Agriculture, Food and Wine, University of Adelaide, Waite Campus, Glen Osmond, SA 5064, Australia
    Carbohydr Res 342:1613-23. 2007
    ....
  6. pmc Hydrolysis of (1,4)-beta-D-mannans in barley (Hordeum vulgare L.) is mediated by the concerted action of (1,4)-beta-D-mannan endohydrolase and beta-D-mannosidase
    Maria Hrmova
    School of Agriculture, Food and Wine, University of Adelaide and Australian Centre for Plant Functional Genomics, Waite Campus, Glen Osmond, SA 5064, Australia
    Biochem J 399:77-90. 2006
    ..Biol. Chem. 273, 11134-11143], which hydrolyses 4NP (4-nitrophenyl) beta-D-mannoside three times faster than 4NP beta-D-glucoside, and has an action pattern typical of a (1,4)-beta-D-mannan exohydrolase...
  7. ncbi request reprint Structural rationale for low-nanomolar binding of transition state mimics to a family GH3 beta-D-glucan glucohydrolase from barley
    Maria Hrmova
    School of Agriculture and Wine and Australian Centre for Plant Functional Genomics, University of Adelaide, Waite Campus, Glen Osmond, SA 5064, Australia
    Biochemistry 44:16529-39. 2005
    ..Finally, the high-resolution structures enabled us to define minimal structures of oligosaccharides attached to Asn221, Asn498, and Asn600 N-glycosylation sites...
  8. ncbi request reprint Three-dimensional structure of the barley beta-D-glucan glucohydrolase in complex with a transition state mimic
    Maria Hrmova
    Faculty of Sciences, School of Agriculture and Wine, University of Adelaide, Waite Campus, Glen Osmond, SA 5064, Australia
    J Biol Chem 279:4970-80. 2004
    ..The data indicate that the 4E conformation of the glucose part of PheGlcIm is critical for tight binding and provide the first evidence for probable substrate distortion during catalysis by this enzyme...
  9. pmc Structural basis for broad substrate specificity in higher plant beta-D-glucan glucohydrolases
    Maria Hrmova
    Department of Plant Science, University of Adelaide, Waite Campus, Glen Osmond, South Australia 5064, Australia
    Plant Cell 14:1033-52. 2002
    ..The broad specificity for glycosidic linkage type enables the enzyme to perform diverse functions during plant development...
  10. pmc The genetics, transcriptional profiles, and catalytic properties of UDP-alpha-D-xylose 4-epimerases from barley
    Qisen Zhang
    Australian Centre for Plant Functional Genomics, School of Agriculture, Food, and Wine, University of Adelaide, Waite Campus, Glen Osmond, South Australia 5064, Australia
    Plant Physiol 153:555-68. 2010
    ..Furthermore, the data revealed that the transcription of HvUXE and HvUXS gene family members is coordinated with the incorporation of pentose sugars onto cell walls in barley leaves, roots, and developing endosperm...
  11. ncbi request reprint Characterization of the wheat gene encoding a grain-specific lipid transfer protein TdPR61, and promoter activity in wheat, barley and rice
    Nataliya Kovalchuk
    Australian Centre for Plant Functional Genomics, University of Adelaide, South Australia, Australia
    J Exp Bot 63:2025-40. 2012
    ..A transcription factor isolated in the screen is similar to BES1/BLZ1 from Arabidopsis, which is known to be a key transcriptional regulator of the brassinosteroid signalling pathway...
  12. ncbi request reprint Defensin promoters as potential tools for engineering disease resistance in cereal grains
    Nataliya Kovalchuk
    Australian Centre for Plant Functional Genomics, Hartley Grove, Urrbrae, SA, Australia
    Plant Biotechnol J 8:47-64. 2010
    ..These results suggest that PRPI promoters will be useful for specific targeting and accumulation of proteins conferring resistance to pathogens in vulnerable tissues of developing and germinating grain...
  13. ncbi request reprint Plant cell wall biosynthesis: genetic, biochemical and functional genomics approaches to the identification of key genes
    Naser Farrokhi
    School of Agriculture and Wine, and Australian Centre for Plant Functional Genomics, University of Adelaide, Waite Campus, Glen Osmond, SA 5064, Australia
    Plant Biotechnol J 4:145-67. 2006
    ....
  14. pmc A two-staged model of Na+ exclusion in rice explained by 3D modeling of HKT transporters and alternative splicing
    Olivier Cotsaftis
    Australian Centre for Plant Functional Genomics, University of Adelaide, Adelaide, South Australia, Australia
    PLoS ONE 7:e39865. 2012
    ..Overall, allelic variation of leaf blade Na(+) accumulation can be explained by a complex interplay of gene transcription, alternative splicing and protein structure...
  15. ncbi request reprint Cellulose synthase-like CslF genes mediate the synthesis of cell wall (1,3;1,4)-beta-D-glucans
    Rachel A Burton
    Australian Centre for Plant Functional Genomics School of Agriculture, Food, and Wine University of Adelaide, Waite Campus, Glen Osmond, SA 5064, Australia
    Science 311:1940-2. 2006
    ....
  16. doi request reprint Functional genomics and structural biology in the definition of gene function
    Maria Hrmova
    Australian Centre for Plant Functional Genomics, School of Agriculture, Food and Wine, University of Adelaide, Waite Campus, Glen Osmond, SA 5064, Australia
    Methods Mol Biol 513:199-227. 2009
    ..In this chapter, we present specific examples of how structural biology can provide valuable information for functional genomics programs...
  17. ncbi request reprint Biochemical evidence linking a putative callose synthase gene with (1 --> 3)-beta-D-glucan biosynthesis in barley
    Jing Li
    Australian Centre for Plant Functional Genomics, University of Adelaide, Waite Campus, Glen Osmond, SA 5064, Australia
    Plant Mol Biol 53:213-25. 2003
    ..Thus, the amino acid sequence predicted from the HvGSL1 gene has been linked with the actual amino acid sequence of an active (1 --> 3)-beta-D-glucan synthase fraction from barley...
  18. ncbi request reprint Bifunctional family 3 glycoside hydrolases from barley with alpha -L-arabinofuranosidase and beta -D-xylosidase activity. Characterization, primary structures, and COOH-terminal processing
    Robert C Lee
    Department of Plant Science, University of Adelaide, Waite Campus, Glen Osmond, South Australia 5064, Australia
    J Biol Chem 278:5377-87. 2003
    ..The genes encoding the ARA-I and XYL have been mapped to chromosomes 2H and 6H, respectively. ARA-I transcripts are most abundant in young roots, young leaves, and developing grain, whereas XYL mRNA is detected in most barley tissues...
  19. pmc Gene expression patterns and catalytic properties of UDP-D-glucose 4-epimerases from barley (Hordeum vulgare L.)
    Qisen Zhang
    Australian Centre for Plant Functional Genomics, School of Agriculture and Wine, University of Adelaide, Waite Campus, Glen Osmond, SA 5064, Australia
    Biochem J 394:115-24. 2006
    ..A three-dimensional model of the HvUGE revealed that its overall structural fold is highly conserved compared with the human UGE and provides a structural rationale for its ability to bind UDP-GlcNAc...
  20. doi request reprint Close allies in membrane protein research: cell-free synthesis and nanotechnology
    Nadim Shadiac
    Australian Centre for Plant Functional Genomics, University of Adelaide, Waite Campus, Glen Osmond SA 5064, Australia
    Mol Membr Biol 30:229-45. 2013
    ....
  21. pmc Complex regulation by Apetala2 domain-containing transcription factors revealed through analysis of the stress-responsive TdCor410b promoter from durum wheat
    Omid Eini
    Australian Centre for Plant Functional Genomics, University of Adelaide, Glen Osmond, South Australia, Australia
    PLoS ONE 8:e58713. 2013
    ..We suggest that both DREB and ERF proteins regulate expression of the Cor410b gene through a single, critical CRT element. Members of the TaERF4 subfamily are specific, positive regulators of Cor410b gene expression...
  22. doi request reprint Cell-free protein synthesis of membrane (1,3)-β-d-glucan (curdlan) synthase: co-translational insertion in liposomes and reconstitution in nanodiscs
    Agalya Periasamy
    Australian Centre for Plant Functional Genomics, School of Agriculture, Food and Wine, University of Adelaide, Waite Campus, Australia
    Biochim Biophys Acta 1828:743-57. 2013
    ..coli lipids to be catalytically competent. Conversely, CrdS synthesised with only Brij-58 was inactive. Our findings pave the way for future structural studies of this industrially important catalytic membrane protein...
  23. ncbi request reprint Characterization of the wheat endosperm transfer cell-specific protein TaPR60
    Nataliya Kovalchuk
    Australian Centre for Plant Functional Genomics, Glen Osmond, SA 5064, Australia
    Plant Mol Biol 71:81-98. 2009
    ..Examination of a three dimensional model of TaPR60 suggested that this protein could be involved in binding of lipidic molecules...
  24. pmc Boron toxicity tolerance in barley through reduced expression of the multifunctional aquaporin HvNIP2;1
    Thorsten Schnurbusch
    Australian Centre for Plant Functional Genomics, University of Adelaide, Waite Campus, Urrbrae, South Australia 5064, Australia
    Plant Physiol 153:1706-15. 2010
    ..Together with Bot1, the multifunctional aquaporin HvNIP2;1 is an important determinant of B toxicity tolerance in barley...
  25. doi request reprint Structural and functional analyses of PpENA1 provide insights into cation binding by type IID P-type ATPases in lower plants and fungi
    Damian P Drew
    Australian Centre for Plant Functional Genomics, University of Adelaide, Waite Campus, Glen Osmond, South Australia 5064, Australia
    Biochim Biophys Acta 1808:1483-92. 2011
    ..Our results suggest that the residues responsible for cation-binding in PpENA1 are distinct from those in fungal type IID ATPases, and that a fungal-type cation binding site can be successfully engineered into the moss protein...
  26. doi request reprint Modulation of plant growth by HD-Zip class I and II transcription factors in response to environmental stimuli
    John C Harris
    Australian Centre for Plant Functional Genomics, PMB 1, Glen Osmond, SA 5064, Australia
    New Phytol 190:823-37. 2011
    ..The in planta implications of these protein-protein associations and the similarities in cis element binding are not clear...
  27. ncbi request reprint A glimpse at regulation of nitrogen homeostasis
    Brent N Kaiser
    School of Agriculture, Food, and Wine, The University of Adelaide, Glen Osmond, SA 5064, Australia
    Structure 18:1395-7. 2010
    ..Structural studies by Wisedchaisri et al. (2010) have identified the cleft region, where 2OG binds to NrpR. Their study has highlighted conservation of 2OG-binding residues among other 2OG-responsive proteins...
  28. pmc Sexual and apomictic seed formation in Hieracium requires the plant polycomb-group gene FERTILIZATION INDEPENDENT ENDOSPERM
    Julio C M Rodrigues
    Commonwealth Scientific and Industrial Research Organization Plant Industry, Glen Osmond, South Australia 5064, Australia
    Plant Cell 20:2372-86. 2008
    ..These differences in protein interaction were attributed to structural modifications predicted from comparisons of Arabidopsis and Hieracium FIE molecular models...
  29. ncbi request reprint Mutated barley (1,3)-beta-D-glucan endohydrolases synthesize crystalline (1,3)-beta-D-glucans
    Maria Hrmova
    Department of Plant Science, University of Adelaide, Waite Campus, Glen Osmond, South Australia 5064, Australia
    J Biol Chem 277:30102-11. 2002
    ....
  30. doi request reprint Transcriptional regulation of cuticle biosynthesis
    Nikolai Borisjuk
    Australian Centre for Plant Functional Genomics, University of Adelaide, Waite Campus, Glen Osmond, South Australia 5064, Australia Electronic address
    Biotechnol Adv 32:526-40. 2014
    ..This review addresses the genetic networks responsible for cuticle formation and in particular highlights the role of transcription factors that regulate cuticle formation in response to abiotic stresses. ..
  31. pmc Role of Homeodomain Leucine Zipper (HD-Zip) IV Transcription Factors in Plant Development and Plant Protection from Deleterious Environmental Factors
    William Chew
    Australian Centre for Plant Functional Genomics, University of Adelaide, Waite Campus, Glen Osmond, South Australia 5064, Australia
    Int J Mol Sci 14:8122-47. 2013
    ..To this end, there is a need for in-depth studies to further clarify the function of each HD-Zip IV subfamily member in commercially important plant species...
  32. pmc Molecular modeling of family GH16 glycoside hydrolases: potential roles for xyloglucan transglucosylases/hydrolases in cell wall modification in the poaceae
    Marco Strohmeier
    Institute of Technical Biochemistry at the University of Stuttgart, D 70569 Stuttgart, Germany
    Protein Sci 13:3200-13. 2004
    ..A role for XTHs in (1,3;1,4)-beta-D-glucan and arabinoxylan modification would be consistent with the apparent overrepresentation of XTH sequences in cereal expressed sequence tags databases...
  33. ncbi request reprint Members of a new group of chitinase-like genes are expressed preferentially in cotton cells with secondary walls
    Deshui Zhang
    Department of Biological Sciences, Texas Tech University, Lubbock, TX 79409, USA
    Plant Mol Biol 54:353-72. 2004
    ..However, the mechanism by which they act is more likely to involve binding of chitin oligosaccharides than catalysis...
  34. ncbi request reprint Reconstitution of cyanogenesis in barley (Hordeum vulgare L.) and its implications for resistance against the barley powdery mildew fungus
    Kirsten A Nielsen
    Plant Biochemistry Laboratory, Department of Plant Biology, Royal Veterinary and Agricultural University, 1871, Frederiksberg C, Copenhagen, Denmark
    Planta 223:1010-23. 2006
    ..graminis in vitro, suggesting that loss of cyanogenesis in barley leaves has enabled the fungus to utilize the presence of epiheterodendrin to facilitate host recognition and to establish infection...
  35. ncbi request reprint Synthesis of complex oligosaccharides by using a mutated (1,3)-beta-D-glucan endohydrolase from barley
    Jon K Fairweather
    Centre de Recherches sur les Macromolecules Vegetales CERMAV CNRS, affiliated with Université J Fourier, B P 53, Grenoble Cedex 9, 38041 France
    Chemistry 9:2603-10. 2003
    ....