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Genomes and Genes | John F HancockSummaryAffiliation: University of Queensland Country: Australia Publications
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Publications
Ras proteins: different signals from different locationsJohn F Hancock
Institute for Molecular Bioscience and Department of Molecular and Cellular Pathology, University of Queensland, Brisbane, Australia 4072
Nat Rev Mol Cell Biol 4:373-84. 2003..These different membrane microenvironments modulate Ras signal output and highlight the complex interplay between Ras location and function...
Evolutionary analysis and molecular dissection of caveola biogenesisMatthew Kirkham
Institute for Molecular Bioscience, University of Queensland, Queensland, Brisbane, Australia
J Cell Sci 121:2075-86. 2008..These studies lead to a model for caveola formation and novel insights into the evolution of caveolin function...
A novel switch region regulates H-ras membrane orientation and signal outputDaniel Abankwa
Molecular Cell Biology, Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland, Australia
EMBO J 27:727-35. 2008..The results illustrate how the plasma membrane spatially constrains signalling conformations by acting as a semi-neutral interaction partner...- PTRF-Cavin, a conserved cytoplasmic protein required for caveola formation and functionMichelle M Hill
Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland 4072, Australia
Cell 132:113-24. 2008..We conclude that PTRF-Cavin is required for caveola formation and sequestration of mobile caveolin into immobile caveolae... - Caveolin regulates endocytosis of the muscle repair protein, dysferlinDelia J Hernandez-Deviez
Institute for Molecular Bioscience, Centre for Microscopy and Microanalysis, University of Queensland, Brisbane, Queensland 4072, Australia
J Biol Chem 283:6476-88. 2008..In addition, they highlight a novel mechanism of action of caveolins relevant to the pathogenic mechanisms underlying caveolin-associated disease... - Caveolin interacts with the angiotensin II type 1 receptor during exocytic transport but not at the plasma membraneBruce D Wyse
Department of Molecular and Cellular Pathology, University of Queensland, Brisbane, Queensland, Australia
J Biol Chem 278:23738-46. 2003..Caveolin therefore acts as a molecular chaperone rather than a plasma membrane scaffold for AT1-R... - Mechanisms of Ras membrane organization and signalling: Ras on a rockerDaniel Abankwa
Institute for Molecular Bioscience, University of Queensland, Brisbane, Australia
Cell Cycle 7:2667-73. 2008..The balance model rationalizes Ras membrane orientation and may help explain isoform specific interactions of Ras with its effectors and modulators... - Electron microscopic imaging of Ras signaling domainsJohn F Hancock
Institute for Molecular Bioscience, University of Queensland, Brisbane, Australia
Methods 37:165-72. 2005..By allowing detailed monitoring of cell surface organization and compartmentalization, the approach has widespread potential for studies of plasma membrane-dependent cell biology, including regulated signaling and membrane trafficking... - Epidermal growth factor receptor activation remodels the plasma membrane lipid environment to induce nanocluster formationNicholas Ariotti
Institute for Molecular Bioscience, University of Queensland, St Lucia, Brisbane, Australia
Mol Cell Biol 30:3795-804. 2010..Together, these data show that EGFR activation drives the formation of signaling domains by regulating the production of critical second-messenger lipids and modifying the local membrane lipid environment... - Ras plasma membrane signalling platformsJohn F Hancock
Institute for Molecular Bioscience, University of Queensland, Brisbane, 4072, Australia
Biochem J 389:1-11. 2005..We will correlate these recent studies suggesting Ras proteins might operate within a heterogeneous plasma membrane with earlier biochemical work on Ras signal transduction... - Ras nanoclusters: combining digital and analog signalingAngus Harding
Queensland Brain Institute, University of Queensland, Brisbane, Australia
Cell Cycle 7:127-34. 2008..In particular, we explore how cells can reconfigure signaling cascades to generate distinct biological outputs by utilizing the unique spatial constraints available in biological membranes... - Lipid rafts and membrane trafficMichael F Hanzal-Bayer
Institute for Molecular Bioscience, University of Queensland, St Lucia 4072, Australia
FEBS Lett 581:2098-104. 2007..We will review their potential involvement in different forms of membrane traffic, explicitly excluding signalling, and discuss select aspects of the raft hypothesis in its current form... - Ras membrane orientation and nanodomain localization generate isoform diversityDaniel Abankwa
The University of Queensland, Institute for Molecular Bioscience, Brisbane 4072, Australia
Proc Natl Acad Sci U S A 107:1130-5. 2010..Finally, we show that G-domain orientation and nanoclustering synergize to generate Ras isoform specificity with respect to effector interactions... - Lipid rafts: contentious only from simplistic standpointsJohn F Hancock
Institute for Molecular Bioscience, University of Queensland, Brisbane 4072, Australia
Nat Rev Mol Cell Biol 7:456-62. 2006..In the light of recent studies with model membranes, computational modelling and innovative cell biology, I propose an updated model of lipid rafts that readily accommodates diverse views on plasma-membrane micro-organization... - Biogenesis of caveolae: a structural model for caveolin-induced domain formationRobert G Parton
Institute for Molecular Bioscience, University of Queensland, Queensland 4072, Australia
J Cell Sci 119:787-96. 2006..Modelling of caveolin-membrane interactions together with in vitro studies of caveolin peptides are providing new insights into how caveolin-lipid interactions could generate the unique architecture of the caveolar domain... - Reassessing the role of phosphocaveolin-1 in cell adhesion and migrationMichelle M Hill
Institute for Molecular Bioscience, University of Queensland, Brisbane, 4072, Australia
Traffic 8:1695-705. 2007..We re-evaluate the current knowledge about the role of caveolin-1 in cell adhesion and migration in view of this new information... - Mtx2 directs zebrafish morphogenetic movements during epiboly by regulating microfilament formationSimon J Wilkins
Institute for Molecular Bioscience, University of Queensland, Brisbane, 4072, Australia
Dev Biol 314:12-22. 2008..In summary, we propose that Mtx2 acts within the YSL to regulate morphogenetic movements of both embryonic and extra-embryonic tissues, independently of cell fate specification... - Using plasma membrane nanoclusters to build better signaling circuitsAngus S Harding
Queensland Brain Institute, University of Queensland, Brisbane, Australia
Trends Cell Biol 18:364-71. 2008..We identify common design principals that highlight how the spatial organization of signal transduction circuits can be used as a fundamental control mechanism to modulate system outputs in vivo... - Identification of residues and domains of Raf important for function in vivo and in vitroAngus Harding
Institute for Molecular Bioscience and Department of Molecular and Cellular Pathology, University of Queensland, Brisbane 4072, Australia
J Biol Chem 278:45519-27. 2003..elegans phenotype... - Direct visualization of Ras proteins in spatially distinct cell surface microdomainsIan A Prior
Department of Pathology and Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland 4006, Australia
J Cell Biol 160:165-70. 2003..Differential spatial localization within this framework can likely account for the distinct signal outputs from the highly homologous Ras proteins... - Plasma membrane nanoswitches generate high-fidelity Ras signal transductionTianhai Tian
Institute for Molecular Bioscience, University of Queensland, Brisbane 4072, Australia
Nat Cell Biol 9:905-14. 2007..A requirement for high-fidelity signalling may explain the non-random distribution of other plasma membrane signalling complexes... - PA promoted to managerJohn F Hancock
Nat Cell Biol 9:615-7. 2007 - Ras nanoclusters: molecular structure and assemblyDaniel Abankwa
Institute for Molecular Bioscience, University of Queensland, Brisbane 4072, Australia
Semin Cell Dev Biol 18:599-607. 2007..We explore possible mechanisms for how the interactions of H-ras with a lipid bilayer may drive nanocluster formation... - K-ras nanoclustering is subverted by overexpression of the scaffold protein galectin-3Ruby Shalom-Feuerstein
Department of Neurobiology, The George S Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel
Cancer Res 68:6608-16. 2008..These results show that Gal-3 overexpression in breast cancer cells, which increases K-Ras signal output, represents oncogenic subversion of plasma membrane nanostructure... - C-terminal sequences in R-Ras are involved in integrin regulation and in plasma membrane microdomain distributionMalene Hansen
Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
Biochem Biophys Res Commun 311:829-38. 2003..However, functionally distinct H-Ras/R-Ras chimeras containing different C-terminal R-Ras segments localized to lipid rafts irrespective of their integrin phenotype... - Electrostatic interactions positively regulate K-Ras nanocluster formation and functionSarah J Plowman
Institute for Molecular Bioscience, University of Queensland, St Lucia, Brisbane 4072, Australia
Mol Cell Biol 28:4377-85. 2008..Together, these data illustrate that Ras nanocluster composition regulates effector recruitment and highlight the importance of lipid/protein nanoscale environments to the activation of signaling cascades... - Human Sin1 contains Ras-binding and pleckstrin homology domains and suppresses Ras signallingWayne A Schroder
The Queensland Institute of Medical Research, Brisbane, Australia
Cell Signal 19:1279-89. 2007..In contrast, siRNA knockdown of endogenous Sin1 protein expression in HEK293 cells enhanced the activation of ERK1/2 by Ras. These data suggest that Sin1 is a mammalian Ras-inhibitor... - Inhibition of lipid raft-dependent signaling by a dystrophy-associated mutant of caveolin-3Amanda J Carozzi
Institute for Molecular Bioscience, Centre for Microscopy and Microanalysis, and School of Biomedical Sciences, University of Queensland, Brisbane 4072, Australia
J Biol Chem 277:17944-9. 2002..Thus, we have demonstrated that a naturally occurring caveolin-3 mutation can inhibit signaling involving cholesterol-sensitive raft domains... - H-Ras signaling and K-Ras signaling are differentially dependent on endocytosisSandrine Roy
Laboratory of Experimental Oncology, Department of Pathology, University of Queensland Medical School, Herston Road, Brisbane 4006, Australia
Mol Cell Biol 22:5128-40. 2002..These results show that H-Ras but not K-Ras signaling though the Raf/MEK/MAPK cascade requires endocytosis and endocytic recycling. The data also suggest a mechanism for returning Raf-1 to the cytosol after plasma membrane recruitment... - Flotillin-1/reggie-2 traffics to surface raft domains via a novel golgi-independent pathway. Identification of a novel membrane targeting domain and a role for palmitoylationIsabel C Morrow
Institute for Molecular Bioscience and Centre for Functional and Applied Genomics, University of Queensland, St Lucia, Australia
J Biol Chem 277:48834-41. 2002..The results identify a novel targeting mechanism for plasma membrane association of flotillin-1 involving a Golgi-independent trafficking pathway, the PHB domain, and palmitoylation... - Lipid rafts and plasma membrane microorganization: insights from RasRobert G Parton
Institute for Molecular Bioscience, Centre for Microscopy and Microanalysis and School of Biomedical Sciences, University of Queensland, QLD4072, Australia
Trends Cell Biol 14:141-7. 2004..This review focuses on some of these recent advances and uses Ras signaling as a paradigm for understanding inner plasma membrane organization and the role of lipid rafts in cellular function... - GPI-anchor synthesis: Ras takes chargeJohn F Hancock
Institute for Molecular Bioscience, University of Queensland Brisbane 4072, Australia
Dev Cell 6:743-5. 2004..Reciprocally, the targeted enzyme GPI-GlcNAc transferase regulates Ras2 signal output. This novel intersection of Ras2 signaling and an ER-localized protein complex has interesting implications for Ras function... - Three separable domains regulate GTP-dependent association of H-ras with the plasma membraneBarak Rotblat
Institute for Molecular Bioscience, 306 Carmody Road, University of Queensland, Brisbane 4072, Australia
Mol Cell Biol 24:6799-810. 2004.... - Ultrastructural identification of uncoated caveolin-independent early endocytic vehiclesMatthew Kirkham
Institute for Molecular Bioscience, University of Queensland, Queensland 4072, Australia
J Cell Biol 168:465-76. 2005..These carriers contained GPI-anchored proteins and fluid phase markers and represented the major vehicles mediating CTB uptake in both WT and caveolae-null cells... - Subcellular localization determines MAP kinase signal outputAngus Harding
Institute for Molecular Bioscience, University of Queensland, Brisbane 4072, Australia
Curr Biol 15:869-73. 2005..These results reveal the engineering logic behind the recruitment of elements of the module from the cytosol to the membrane for activation... - Sources of anomalous diffusion on cell membranes: a Monte Carlo studyDan V Nicolau
Advanced Computational Modelling Centre, Department of Mathematics, and Institute for Molecular Bioscience, University of Queensland, St Lucia, Australia
Biophys J 92:1975-87. 2007.... - The linker domain of the Ha-Ras hypervariable region regulates interactions with exchange factors, Raf-1 and phosphoinositide 3-kinaseMontserrat Jaumot
Laboratory of Experimental Oncology, Department of Pathology, University of Queensland Medical School, Herston Road, Queensland 4006, Australia
J Biol Chem 277:272-8. 2002..Mutations in the linker domain also suppress the dominant negative phenotype of Ha-RasS17N, indicating that HVR sequences are essential for efficient interaction of Ha-Ras with exchange factors in intact cells... - Individual palmitoyl residues serve distinct roles in H-ras trafficking, microlocalization, and signalingSandrine Roy
Institute for Molecular Bioscience, 306 Carmody Road, University of Queensland, Brisbane 4072, Australia
Mol Cell Biol 25:6722-33. 2005..Finally we show that the plasma membrane affinity of monopalmitoylated anchors is absolutely dependent on cholesterol, identifying a new role for cholesterol in promoting interactions with the raft and nonraft plasma membrane... - Identifying optimal lipid raft characteristics required to promote nanoscale protein-protein interactions on the plasma membraneDan V Nicolau
Advanced Computational Modelling Centre, Department of Mathematics, University of Queensland, Brisbane 4072, Australia
Mol Cell Biol 26:313-23. 2006..These results may have significant implications for the many signaling cascades that are scaffolded or assembled in plasma membrane microdomains... - H-ras, K-ras, and inner plasma membrane raft proteins operate in nanoclusters with differential dependence on the actin cytoskeletonSarah J Plowman
Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis and School of Biomedical Sciences, University of Queensland, Brisbane 4072, Australia
Proc Natl Acad Sci U S A 102:15500-5. 2005..RasG12V nanoclusters may facilitate the assembly of essential signal transduction complexes... - An agonist-induced conformational change in the growth hormone receptor determines the choice of signalling pathwayScott W Rowlinson
Institute for Molecular Bioscience and School of Biomedical Sciences, University of Queensland, St Lucia 4072, Australia
Nat Cell Biol 10:740-7. 2008..Our findings could explain debilitated ERK but not STAT5 signalling in some GH-resistant dwarfs and suggest pathway-specific cytokine agonists... - Observing cell surface signaling domains using electron microscopyIan A Prior
The Physiological Laboratory, University of Liverpool, UK
Sci STKE 2003:PL9. 2003..The approach has enormous potential to determine plasma membrane organization and the spatial dynamics of regulated signaling and membrane trafficking events associated with the cell surface... - Structure and dynamics of the full-length lipid-modified H-Ras protein in a 1,2-dimyristoylglycero-3-phosphocholine bilayerAlemayehu A Gorfe
Department of Chemistry and Biochemistry, Center for Theoretical Biological Physics, Howard Hughes Medical Institute, University of California at San Diego, La Jolla, California 92093 0365, USA
J Med Chem 50:674-84. 2007..The implications of the results to membrane lateral segregation and other aspects of Ras signaling are discussed... - Cholesterol-sensitive Cdc42 activation regulates actin polymerization for endocytosis via the GEEC pathwayRahul Chadda
National Centre for Biological Sciences, UAS GKVK Campus, Bellary Road, Bangalore 560065, India
Traffic 8:702-17. 2007..These results suggest that endocytosis into GEECs occurs through a cholesterol-sensitive, Cdc42-based recruitment of the actin polymerization machinery... - Mechanism of mitosis-specific activation of MEK1Angus Harding
Cancer Biology Program, Centre for Immunology and Cancer Research, University of Queensland, Queensland 4102, Australia
J Biol Chem 278:16747-54. 2003..These results demonstrate that cyclin B-Cdc2 activity regulates signaling through the MAPK pathway in mitosis...