John F Hancock

Summary

Affiliation: University of Queensland
Country: Australia

Publications

  1. pmc Direct visualization of Ras proteins in spatially distinct cell surface microdomains
    Ian A Prior
    Department of Pathology and Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland 4006, Australia
    J Cell Biol 160:165-70. 2003
  2. ncbi request reprint Ras proteins: different signals from different locations
    John F Hancock
    Institute for Molecular Bioscience and Department of Molecular and Cellular Pathology, University of Queensland, Brisbane, Australia 4072
    Nat Rev Mol Cell Biol 4:373-84. 2003
  3. doi request reprint Evolutionary analysis and molecular dissection of caveola biogenesis
    Matthew Kirkham
    Institute for Molecular Bioscience, University of Queensland, Queensland, Brisbane, Australia
    J Cell Sci 121:2075-86. 2008
  4. pmc Individual palmitoyl residues serve distinct roles in H-ras trafficking, microlocalization, and signaling
    Sandrine Roy
    Institute for Molecular Bioscience, 306 Carmody Road, University of Queensland, Brisbane 4072, Australia
    Mol Cell Biol 25:6722-33. 2005
  5. pmc Activation of the MAPK module from different spatial locations generates distinct system outputs
    Kerry Inder
    Institute for Molecular Bioscience, University of Queensland, Brisbane 4072, Australia
    Mol Biol Cell 19:4776-84. 2008
  6. pmc PTRF-Cavin, a conserved cytoplasmic protein required for caveola formation and function
    Michelle M Hill
    Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland 4072, Australia
    Cell 132:113-24. 2008
  7. ncbi request reprint Plasma membrane nanoswitches generate high-fidelity Ras signal transduction
    Tianhai Tian
    Institute for Molecular Bioscience, University of Queensland, Brisbane 4072, Australia
    Nat Cell Biol 9:905-14. 2007
  8. pmc Three separable domains regulate GTP-dependent association of H-ras with the plasma membrane
    Barak Rotblat
    Institute for Molecular Bioscience, 306 Carmody Road, University of Queensland, Brisbane 4072, Australia
    Mol Cell Biol 24:6799-810. 2004
  9. pmc A novel switch region regulates H-ras membrane orientation and signal output
    Daniel Abankwa
    Molecular Cell Biology, Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland, Australia
    EMBO J 27:727-35. 2008
  10. pmc MURC/Cavin-4 and cavin family members form tissue-specific caveolar complexes
    Michele Bastiani
    Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland 4072, Australia
    J Cell Biol 185:1259-73. 2009

Collaborators

Detail Information

Publications54

  1. pmc Direct visualization of Ras proteins in spatially distinct cell surface microdomains
    Ian A Prior
    Department of Pathology and Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland 4006, Australia
    J Cell Biol 160:165-70. 2003
    ..Differential spatial localization within this framework can likely account for the distinct signal outputs from the highly homologous Ras proteins...
  2. ncbi request reprint Ras proteins: different signals from different locations
    John F Hancock
    Institute for Molecular Bioscience and Department of Molecular and Cellular Pathology, University of Queensland, Brisbane, Australia 4072
    Nat Rev Mol Cell Biol 4:373-84. 2003
    ..These different membrane microenvironments modulate Ras signal output and highlight the complex interplay between Ras location and function...
  3. doi request reprint Evolutionary analysis and molecular dissection of caveola biogenesis
    Matthew Kirkham
    Institute for Molecular Bioscience, University of Queensland, Queensland, Brisbane, Australia
    J Cell Sci 121:2075-86. 2008
    ..These studies lead to a model for caveola formation and novel insights into the evolution of caveolin function...
  4. pmc Individual palmitoyl residues serve distinct roles in H-ras trafficking, microlocalization, and signaling
    Sandrine Roy
    Institute for Molecular Bioscience, 306 Carmody Road, University of Queensland, Brisbane 4072, Australia
    Mol Cell Biol 25:6722-33. 2005
    ..Finally we show that the plasma membrane affinity of monopalmitoylated anchors is absolutely dependent on cholesterol, identifying a new role for cholesterol in promoting interactions with the raft and nonraft plasma membrane...
  5. pmc Activation of the MAPK module from different spatial locations generates distinct system outputs
    Kerry Inder
    Institute for Molecular Bioscience, University of Queensland, Brisbane 4072, Australia
    Mol Biol Cell 19:4776-84. 2008
    ....
  6. pmc PTRF-Cavin, a conserved cytoplasmic protein required for caveola formation and function
    Michelle M Hill
    Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland 4072, Australia
    Cell 132:113-24. 2008
    ..We conclude that PTRF-Cavin is required for caveola formation and sequestration of mobile caveolin into immobile caveolae...
  7. ncbi request reprint Plasma membrane nanoswitches generate high-fidelity Ras signal transduction
    Tianhai Tian
    Institute for Molecular Bioscience, University of Queensland, Brisbane 4072, Australia
    Nat Cell Biol 9:905-14. 2007
    ..A requirement for high-fidelity signalling may explain the non-random distribution of other plasma membrane signalling complexes...
  8. pmc Three separable domains regulate GTP-dependent association of H-ras with the plasma membrane
    Barak Rotblat
    Institute for Molecular Bioscience, 306 Carmody Road, University of Queensland, Brisbane 4072, Australia
    Mol Cell Biol 24:6799-810. 2004
    ....
  9. pmc A novel switch region regulates H-ras membrane orientation and signal output
    Daniel Abankwa
    Molecular Cell Biology, Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland, Australia
    EMBO J 27:727-35. 2008
    ..The results illustrate how the plasma membrane spatially constrains signalling conformations by acting as a semi-neutral interaction partner...
  10. pmc MURC/Cavin-4 and cavin family members form tissue-specific caveolar complexes
    Michele Bastiani
    Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland 4072, Australia
    J Cell Biol 185:1259-73. 2009
    ..Cavin-4 is expressed predominantly in muscle, and its distribution is perturbed in human muscle disease associated with Caveolin-3 dysfunction, identifying Cavin-4 as a novel muscle disease candidate caveolar protein...
  11. pmc Nucleophosmin and nucleolin regulate K-Ras plasma membrane interactions and MAPK signal transduction
    Kerry L Inder
    Institute for Molecular Bioscience, University of Queensland, Brisbane 4072, Australia
    J Biol Chem 284:28410-9. 2009
    ..The study also identifies a new class of K-Ras nanocluster regulator that operates independently of the structural scaffold galectin-3...
  12. pmc Clathrin-independent carriers form a high capacity endocytic sorting system at the leading edge of migrating cells
    Mark T Howes
    The Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, The University of Queensland, Brisbane, Queensland 4072, Australia
    J Cell Biol 190:675-91. 2010
    ..These studies provide the first quantitative ultrastructural analysis and molecular characterization of the major endocytic pathway in fibroblasts, a pathway that provides rapid membrane turnover at the leading edge of migrating cells...
  13. pmc Co-regulation of cell polarization and migration by caveolar proteins PTRF/Cavin-1 and caveolin-1
    Michelle M Hill
    The University of Queensland Diamantina Institute, The University of Queensland, Brisbane, Queensland, Australia
    PLoS ONE 7:e43041. 2012
    ..By selectively manipulating PTRF/cavin-1 and caveolin-1 expression (and therefore caveola formation) in multiple cell systems, we unveil caveola-independent functions for both proteins in cell migration...
  14. pmc Electrostatic interactions positively regulate K-Ras nanocluster formation and function
    Sarah J Plowman
    Institute for Molecular Bioscience, University of Queensland, St Lucia, Brisbane 4072, Australia
    Mol Cell Biol 28:4377-85. 2008
    ..Together, these data illustrate that Ras nanocluster composition regulates effector recruitment and highlight the importance of lipid/protein nanoscale environments to the activation of signaling cascades...
  15. pmc Ultrastructural identification of uncoated caveolin-independent early endocytic vehicles
    Matthew Kirkham
    Institute for Molecular Bioscience, University of Queensland, Queensland 4072, Australia
    J Cell Biol 168:465-76. 2005
    ..These carriers contained GPI-anchored proteins and fluid phase markers and represented the major vehicles mediating CTB uptake in both WT and caveolae-null cells...
  16. pmc Epidermal growth factor receptor activation remodels the plasma membrane lipid environment to induce nanocluster formation
    Nicholas Ariotti
    Institute for Molecular Bioscience, University of Queensland, St Lucia, Brisbane, Australia
    Mol Cell Biol 30:3795-804. 2010
    ..Together, these data show that EGFR activation drives the formation of signaling domains by regulating the production of critical second-messenger lipids and modifying the local membrane lipid environment...
  17. ncbi request reprint Caveolin interacts with the angiotensin II type 1 receptor during exocytic transport but not at the plasma membrane
    Bruce D Wyse
    Department of Molecular and Cellular Pathology, University of Queensland, Brisbane, Queensland, Australia
    J Biol Chem 278:23738-46. 2003
    ..Caveolin therefore acts as a molecular chaperone rather than a plasma membrane scaffold for AT1-R...
  18. ncbi request reprint Caveolin regulates endocytosis of the muscle repair protein, dysferlin
    Delia J Hernandez-Deviez
    Institute for Molecular Bioscience, Centre for Microscopy and Microanalysis, University of Queensland, Brisbane, Queensland 4072, Australia
    J Biol Chem 283:6476-88. 2008
    ..In addition, they highlight a novel mechanism of action of caveolins relevant to the pathogenic mechanisms underlying caveolin-associated disease...
  19. ncbi request reprint Subcellular localization determines MAP kinase signal output
    Angus Harding
    Institute for Molecular Bioscience, University of Queensland, Brisbane 4072, Australia
    Curr Biol 15:869-73. 2005
    ..These results reveal the engineering logic behind the recruitment of elements of the module from the cytosol to the membrane for activation...
  20. ncbi request reprint Human Sin1 contains Ras-binding and pleckstrin homology domains and suppresses Ras signalling
    Wayne A Schroder
    The Queensland Institute of Medical Research, Brisbane, Australia
    Cell Signal 19:1279-89. 2007
    ..In contrast, siRNA knockdown of endogenous Sin1 protein expression in HEK293 cells enhanced the activation of ERK1/2 by Ras. These data suggest that Sin1 is a mammalian Ras-inhibitor...
  21. ncbi request reprint Inhibition of lipid raft-dependent signaling by a dystrophy-associated mutant of caveolin-3
    Amanda J Carozzi
    Institute for Molecular Bioscience, Centre for Microscopy and Microanalysis, and School of Biomedical Sciences, University of Queensland, Brisbane 4072, Australia
    J Biol Chem 277:17944-9. 2002
    ..Thus, we have demonstrated that a naturally occurring caveolin-3 mutation can inhibit signaling involving cholesterol-sensitive raft domains...
  22. pmc Sources of anomalous diffusion on cell membranes: a Monte Carlo study
    Dan V Nicolau
    Advanced Computational Modelling Centre, Department of Mathematics, and Institute for Molecular Bioscience, University of Queensland, St Lucia, Australia
    Biophys J 92:1975-87. 2007
    ....
  23. pmc The effects of transmembrane sequence and dimerization on cleavage of the p75 neurotrophin receptor by γ-secretase
    Alex M Sykes
    Queensland Brain Institute, University of Queensland, Brisbane, Queensland 4072, Australia
    J Biol Chem 287:43810-24. 2012
    ..These results provide support for the idea that the helical structure of the p75(NTR) transmembrane domain, which may be affected by co-receptor interactions, is a key element in γ-secretase-catalyzed cleavage...
  24. ncbi request reprint Flotillin-1/reggie-2 traffics to surface raft domains via a novel golgi-independent pathway. Identification of a novel membrane targeting domain and a role for palmitoylation
    Isabel C Morrow
    Institute for Molecular Bioscience and Centre for Functional and Applied Genomics, University of Queensland, St Lucia, Australia
    J Biol Chem 277:48834-41. 2002
    ..The results identify a novel targeting mechanism for plasma membrane association of flotillin-1 involving a Golgi-independent trafficking pathway, the PHB domain, and palmitoylation...
  25. ncbi request reprint Lipid rafts and membrane traffic
    Michael F Hanzal-Bayer
    Institute for Molecular Bioscience, University of Queensland, St Lucia 4072, Australia
    FEBS Lett 581:2098-104. 2007
    ..We will review their potential involvement in different forms of membrane traffic, explicitly excluding signalling, and discuss select aspects of the raft hypothesis in its current form...
  26. pmc Identifying optimal lipid raft characteristics required to promote nanoscale protein-protein interactions on the plasma membrane
    Dan V Nicolau
    Advanced Computational Modelling Centre, Department of Mathematics, University of Queensland, Brisbane 4072, Australia
    Mol Cell Biol 26:313-23. 2006
    ..These results may have significant implications for the many signaling cascades that are scaffolded or assembled in plasma membrane microdomains...
  27. pmc H-ras, K-ras, and inner plasma membrane raft proteins operate in nanoclusters with differential dependence on the actin cytoskeleton
    Sarah J Plowman
    Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis and School of Biomedical Sciences, University of Queensland, Brisbane 4072, Australia
    Proc Natl Acad Sci U S A 102:15500-5. 2005
    ..RasG12V nanoclusters may facilitate the assembly of essential signal transduction complexes...
  28. doi request reprint Constitutive formation of caveolae in a bacterium
    Piers J Walser
    The University of Queensland, Institute for Molecular Bioscience, Brisbane, Queensland 4072, Australia
    Cell 150:752-63. 2012
    ..Periplasmic solutes are encapsulated in the budding h-caveola, and purified h-caveolae can be tailored to be targeted to specific cells of interest...
  29. pmc Ras nanoclusters: molecular structure and assembly
    Daniel Abankwa
    Institute for Molecular Bioscience, University of Queensland, Brisbane 4072, Australia
    Semin Cell Dev Biol 18:599-607. 2007
    ..We explore possible mechanisms for how the interactions of H-ras with a lipid bilayer may drive nanocluster formation...
  30. ncbi request reprint Reassessing the role of phosphocaveolin-1 in cell adhesion and migration
    Michelle M Hill
    Institute for Molecular Bioscience, University of Queensland, Brisbane, 4072, Australia
    Traffic 8:1695-705. 2007
    ..We re-evaluate the current knowledge about the role of caveolin-1 in cell adhesion and migration in view of this new information...
  31. pmc Ras nanoclusters: combining digital and analog signaling
    Angus Harding
    Queensland Brain Institute, University of Queensland, Brisbane, Australia
    Cell Cycle 7:127-34. 2008
    ..In particular, we explore how cells can reconfigure signaling cascades to generate distinct biological outputs by utilizing the unique spatial constraints available in biological membranes...
  32. pmc Electron microscopic imaging of Ras signaling domains
    John F Hancock
    Institute for Molecular Bioscience, University of Queensland, Brisbane, Australia
    Methods 37:165-72. 2005
    ..By allowing detailed monitoring of cell surface organization and compartmentalization, the approach has widespread potential for studies of plasma membrane-dependent cell biology, including regulated signaling and membrane trafficking...
  33. pmc Mechanisms of Ras membrane organization and signalling: Ras on a rocker
    Daniel Abankwa
    Institute for Molecular Bioscience, University of Queensland, Brisbane, Australia
    Cell Cycle 7:2667-73. 2008
    ..The balance model rationalizes Ras membrane orientation and may help explain isoform specific interactions of Ras with its effectors and modulators...
  34. ncbi request reprint Lipid rafts and plasma membrane microorganization: insights from Ras
    Robert G Parton
    Institute for Molecular Bioscience, Centre for Microscopy and Microanalysis and School of Biomedical Sciences, University of Queensland, QLD4072, Australia
    Trends Cell Biol 14:141-7. 2004
    ..This review focuses on some of these recent advances and uses Ras signaling as a paradigm for understanding inner plasma membrane organization and the role of lipid rafts in cellular function...
  35. ncbi request reprint Identification of residues and domains of Raf important for function in vivo and in vitro
    Angus Harding
    Institute for Molecular Bioscience and Department of Molecular and Cellular Pathology, University of Queensland, Brisbane 4072, Australia
    J Biol Chem 278:45519-27. 2003
    ..elegans phenotype...
  36. ncbi request reprint Caveolin-1 is necessary for hepatic oxidative lipid metabolism: evidence for crosstalk between caveolin-1 and bile acid signaling
    Manuel A Fernandez-Rojo
    Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland 4067, Australia
    Cell Rep 4:238-47. 2013
    ..These results demonstrate the significance of CAV1 in (1) hepatic lipid homeostasis and (2) nuclear hormone receptor (PPARα, FXRα, and SHP) and bile acid signaling. ..
  37. ncbi request reprint Mtx2 directs zebrafish morphogenetic movements during epiboly by regulating microfilament formation
    Simon J Wilkins
    Institute for Molecular Bioscience, University of Queensland, Brisbane, 4072, Australia
    Dev Biol 314:12-22. 2008
    ..In summary, we propose that Mtx2 acts within the YSL to regulate morphogenetic movements of both embryonic and extra-embryonic tissues, independently of cell fate specification...
  38. pmc Ras plasma membrane signalling platforms
    John F Hancock
    Institute for Molecular Bioscience, University of Queensland, Brisbane, 4072, Australia
    Biochem J 389:1-11. 2005
    ..We will correlate these recent studies suggesting Ras proteins might operate within a heterogeneous plasma membrane with earlier biochemical work on Ras signal transduction...
  39. pmc Ras membrane orientation and nanodomain localization generate isoform diversity
    Daniel Abankwa
    The University of Queensland, Institute for Molecular Bioscience, Brisbane 4072, Australia
    Proc Natl Acad Sci U S A 107:1130-5. 2010
    ..Finally, we show that G-domain orientation and nanoclustering synergize to generate Ras isoform specificity with respect to effector interactions...
  40. ncbi request reprint Biogenesis of caveolae: a structural model for caveolin-induced domain formation
    Robert G Parton
    Institute for Molecular Bioscience, University of Queensland, Queensland 4072, Australia
    J Cell Sci 119:787-96. 2006
    ..Modelling of caveolin-membrane interactions together with in vitro studies of caveolin peptides are providing new insights into how caveolin-lipid interactions could generate the unique architecture of the caveolar domain...
  41. ncbi request reprint The linker domain of the Ha-Ras hypervariable region regulates interactions with exchange factors, Raf-1 and phosphoinositide 3-kinase
    Montserrat Jaumot
    Laboratory of Experimental Oncology, Department of Pathology, University of Queensland Medical School, Herston Road, Queensland 4006, Australia
    J Biol Chem 277:272-8. 2002
    ..Mutations in the linker domain also suppress the dominant negative phenotype of Ha-RasS17N, indicating that HVR sequences are essential for efficient interaction of Ha-Ras with exchange factors in intact cells...
  42. pmc Structure-based reassessment of the caveolin signaling model: do caveolae regulate signaling through caveolin-protein interactions?
    Brett M Collins
    The Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD 4072, Australia
    Dev Cell 23:11-20. 2012
    ..We propose that CBM/CSD-dependent interactions are unlikely to mediate caveolar signaling, and the basis for signaling effects should therefore be reassessed...
  43. pmc Using plasma membrane nanoclusters to build better signaling circuits
    Angus S Harding
    Queensland Brain Institute, University of Queensland, Brisbane, Australia
    Trends Cell Biol 18:364-71. 2008
    ..We identify common design principals that highlight how the spatial organization of signal transduction circuits can be used as a fundamental control mechanism to modulate system outputs in vivo...
  44. doi request reprint An agonist-induced conformational change in the growth hormone receptor determines the choice of signalling pathway
    Scott W Rowlinson
    Institute for Molecular Bioscience and School of Biomedical Sciences, University of Queensland, St Lucia 4072, Australia
    Nat Cell Biol 10:740-7. 2008
    ..Our findings could explain debilitated ERK but not STAT5 signalling in some GH-resistant dwarfs and suggest pathway-specific cytokine agonists...
  45. pmc Lipid rafts: contentious only from simplistic standpoints
    John F Hancock
    Institute for Molecular Bioscience, University of Queensland, Brisbane 4072, Australia
    Nat Rev Mol Cell Biol 7:456-62. 2006
    ..In the light of recent studies with model membranes, computational modelling and innovative cell biology, I propose an updated model of lipid rafts that readily accommodates diverse views on plasma-membrane micro-organization...
  46. pmc H-Ras signaling and K-Ras signaling are differentially dependent on endocytosis
    Sandrine Roy
    Laboratory of Experimental Oncology, Department of Pathology, University of Queensland Medical School, Herston Road, Brisbane 4006, Australia
    Mol Cell Biol 22:5128-40. 2002
    ..These results show that H-Ras but not K-Ras signaling though the Raf/MEK/MAPK cascade requires endocytosis and endocytic recycling. The data also suggest a mechanism for returning Raf-1 to the cytosol after plasma membrane recruitment...
  47. ncbi request reprint C-terminal sequences in R-Ras are involved in integrin regulation and in plasma membrane microdomain distribution
    Malene Hansen
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Biochem Biophys Res Commun 311:829-38. 2003
    ..However, functionally distinct H-Ras/R-Ras chimeras containing different C-terminal R-Ras segments localized to lipid rafts irrespective of their integrin phenotype...
  48. ncbi request reprint GPI-anchor synthesis: Ras takes charge
    John F Hancock
    Institute for Molecular Bioscience, University of Queensland Brisbane 4072, Australia
    Dev Cell 6:743-5. 2004
    ..Reciprocally, the targeted enzyme GPI-GlcNAc transferase regulates Ras2 signal output. This novel intersection of Ras2 signaling and an ER-localized protein complex has interesting implications for Ras function...
  49. pmc K-ras nanoclustering is subverted by overexpression of the scaffold protein galectin-3
    Ruby Shalom-Feuerstein
    Department of Neurobiology, The George S Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel
    Cancer Res 68:6608-16. 2008
    ..These results show that Gal-3 overexpression in breast cancer cells, which increases K-Ras signal output, represents oncogenic subversion of plasma membrane nanostructure...
  50. ncbi request reprint PA promoted to manager
    John F Hancock
    Nat Cell Biol 9:615-7. 2007
  51. ncbi request reprint Structure and dynamics of the full-length lipid-modified H-Ras protein in a 1,2-dimyristoylglycero-3-phosphocholine bilayer
    Alemayehu A Gorfe
    Department of Chemistry and Biochemistry, Center for Theoretical Biological Physics, Howard Hughes Medical Institute, University of California at San Diego, La Jolla, California 92093 0365, USA
    J Med Chem 50:674-84. 2007
    ..The implications of the results to membrane lateral segregation and other aspects of Ras signaling are discussed...
  52. ncbi request reprint Mechanism of mitosis-specific activation of MEK1
    Angus Harding
    Cancer Biology Program, Centre for Immunology and Cancer Research, University of Queensland, Queensland 4102, Australia
    J Biol Chem 278:16747-54. 2003
    ..These results demonstrate that cyclin B-Cdc2 activity regulates signaling through the MAPK pathway in mitosis...
  53. ncbi request reprint Cholesterol-sensitive Cdc42 activation regulates actin polymerization for endocytosis via the GEEC pathway
    Rahul Chadda
    National Centre for Biological Sciences, UAS GKVK Campus, Bellary Road, Bangalore 560065, India
    Traffic 8:702-17. 2007
    ..These results suggest that endocytosis into GEECs occurs through a cholesterol-sensitive, Cdc42-based recruitment of the actin polymerization machinery...
  54. ncbi request reprint Observing cell surface signaling domains using electron microscopy
    Ian A Prior
    The Physiological Laboratory, University of Liverpool, UK
    Sci STKE 2003:PL9. 2003
    ..The approach has enormous potential to determine plasma membrane organization and the spatial dynamics of regulated signaling and membrane trafficking events associated with the cell surface...