Katharina Gaus

Summary

Affiliation: University of New South Wales
Country: Australia

Publications

  1. ncbi request reprint Visualizing membrane microdomains by Laurdan 2-photon microscopy
    Katharina Gaus
    Centre for Vascular Research at the School of Medical Sciences, University of New South Wales and the Department of Haematology, Prince of Wales Hospital, Sydney, Australia
    Mol Membr Biol 23:41-8. 2006
  2. pmc Integrin-mediated adhesion regulates membrane order
    Katharina Gaus
    Centre for Vascular Research, School of Medical Sciences, University of New South Wales, Sydney, 2052 NSW, Australia
    J Cell Biol 174:725-34. 2006
  3. pmc How does the kinase Lck phosphorylate the T cell receptor? Spatial organization as a regulatory mechanism
    Jérémie Rossy
    Centre for Vascular Research, University of New South Wales, Sydney, NSW, Australia
    Front Immunol 3:167. 2012
  4. pmc How do cells make decisions: engineering micro- and nanoenvironments for cell migration
    Siti Hawa Ngalim
    Centre for Vascular Research, University of New South Wales, Sydney 2052 NSW, Australia
    J Oncol 2010:363106. 2010
  5. pmc Caveolin-1-mediated apolipoprotein A-I membrane binding sites are not required for cholesterol efflux
    Soazig Le Lay
    Centre de Recherche des Cordeliers, INSERM, U872, Paris, France
    PLoS ONE 6:e23353. 2011
  6. pmc The integration of signaling and the spatial organization of the T cell synapse
    Jérémie Rossy
    Centre for Vascular Research and Australian Centre for NanoMedicine, University of New South Wales Sydney, NSW, Australia
    Front Immunol 3:352. 2012
  7. pmc Caveolin-1-dependent and -independent membrane domains
    Soazig Le Lay
    Centre de Recherche des Cordeliers, INSERM, U872, Universite Pierre et Marie Curie, Paris 6, France
    J Lipid Res 50:1609-20. 2009
  8. pmc The Reorientation of T-Cell Polarity and Inhibition of Immunological Synapse Formation by CD46 Involves Its Recruitment to Lipid Rafts
    Mandy J Ludford-Menting
    Peter MacCallum Cancer Centre, East Melbourne, VIC 3002, Australia
    J Lipids 2011:521863. 2011
  9. pmc HIV taken by STORM: super-resolution fluorescence microscopy of a viral infection
    Candida F Pereira
    Centre for Virology, Burnet Institute, Melbourne, Australia
    Virol J 9:84. 2012
  10. pmc Condensation of the plasma membrane at the site of T lymphocyte activation
    Katharina Gaus
    Centre for Vascular Research at the School of Medical Sciences, University of New South Wales, Sydney 2052 NSW, Australia
    J Cell Biol 171:121-31. 2005

Collaborators

Detail Information

Publications71

  1. ncbi request reprint Visualizing membrane microdomains by Laurdan 2-photon microscopy
    Katharina Gaus
    Centre for Vascular Research at the School of Medical Sciences, University of New South Wales and the Department of Haematology, Prince of Wales Hospital, Sydney, Australia
    Mol Membr Biol 23:41-8. 2006
    ..Moreover we discuss how microdomains identified by Laurdan microscopy are consistent with domains identified by other methodologies and put GP images in the context of current raft hypotheses...
  2. pmc Integrin-mediated adhesion regulates membrane order
    Katharina Gaus
    Centre for Vascular Research, School of Medical Sciences, University of New South Wales, Sydney, 2052 NSW, Australia
    J Cell Biol 174:725-34. 2006
    ..These results show that lipid raft properties are conferred by assembly of specific protein complexes. The ordered state within focal adhesions may have important consequences for signaling at these sites...
  3. pmc How does the kinase Lck phosphorylate the T cell receptor? Spatial organization as a regulatory mechanism
    Jérémie Rossy
    Centre for Vascular Research, University of New South Wales, Sydney, NSW, Australia
    Front Immunol 3:167. 2012
    ..Here, we discuss the factors that are thought to regulate the spatial distribution of the TCR and Lck, and therefore critically influence TCR signaling initiation...
  4. pmc How do cells make decisions: engineering micro- and nanoenvironments for cell migration
    Siti Hawa Ngalim
    Centre for Vascular Research, University of New South Wales, Sydney 2052 NSW, Australia
    J Oncol 2010:363106. 2010
    ..Controlling both adhesive and soluble cues for migration may allow us to decipher how cells become motile, choose the direction for migration, and how oncogenic transformations influences these decision-making processes...
  5. pmc Caveolin-1-mediated apolipoprotein A-I membrane binding sites are not required for cholesterol efflux
    Soazig Le Lay
    Centre de Recherche des Cordeliers, INSERM, U872, Paris, France
    PLoS ONE 6:e23353. 2011
    ....
  6. pmc The integration of signaling and the spatial organization of the T cell synapse
    Jérémie Rossy
    Centre for Vascular Research and Australian Centre for NanoMedicine, University of New South Wales Sydney, NSW, Australia
    Front Immunol 3:352. 2012
    ..Single molecule super-resolution microscopy may become an important tool in decoding receptor organization at the molecular level...
  7. pmc Caveolin-1-dependent and -independent membrane domains
    Soazig Le Lay
    Centre de Recherche des Cordeliers, INSERM, U872, Universite Pierre et Marie Curie, Paris 6, France
    J Lipid Res 50:1609-20. 2009
    ..In contrast, the abundance of ordered domains in adherent cells is reduced in Cav1(-/-) compared with WT MEFs, suggesting that cell architecture is critical in maintaining Cav1-induced lipid rafts...
  8. pmc The Reorientation of T-Cell Polarity and Inhibition of Immunological Synapse Formation by CD46 Involves Its Recruitment to Lipid Rafts
    Mandy J Ludford-Menting
    Peter MacCallum Cancer Centre, East Melbourne, VIC 3002, Australia
    J Lipids 2011:521863. 2011
    ....
  9. pmc HIV taken by STORM: super-resolution fluorescence microscopy of a viral infection
    Candida F Pereira
    Centre for Virology, Burnet Institute, Melbourne, Australia
    Virol J 9:84. 2012
    ....
  10. pmc Condensation of the plasma membrane at the site of T lymphocyte activation
    Katharina Gaus
    Centre for Vascular Research at the School of Medical Sciences, University of New South Wales, Sydney 2052 NSW, Australia
    J Cell Biol 171:121-31. 2005
    ..The formation of condensed membrane domains at T cell activation sites biophysically reflects membrane raft accumulation, which has potential implications for signaling at ISs...
  11. ncbi request reprint Domain-specific lipid distribution in macrophage plasma membranes
    Katharina Gaus
    Centre for Vascular Research at the School of Medical Sciences, University of New South Wales, and Department of Haematology, Prince of Wales Hospital, Sydney, NSW Australia
    J Lipid Res 46:1526-38. 2005
    ..The role of rafts in regulating the biological activities of macrophage plasma membrane proteins may require careful reevaluation using multiple isolation procedures, analyses of lipids, and microscopic techniques...
  12. pmc Visualizing lipid structure and raft domains in living cells with two-photon microscopy
    Katharina Gaus
    Centre for Vascular Research at the University of New South Wales and Department of Haematology, Prince of Wales Hospital, Sydney NSW 2052, Australia
    Proc Natl Acad Sci U S A 100:15554-9. 2003
    ....
  13. doi request reprint PALM imaging and cluster analysis of protein heterogeneity at the cell surface
    Dylan M Owen
    Centre for Vascular Research, University of New South Wales, Sydney, New South Wales, Australia
    J Biophotonics 3:446-54. 2010
    ..These methods are generally applicable to other cell and fluorophore systems to quantify 2-D molecular clustering at nanometer scales...
  14. pmc Spacing of integrin ligands influences signal transduction in endothelial cells
    Guillaume Le Saux
    Centre for Vascular Research, University of New South Wales, Sydney, Australia
    Biophys J 101:764-73. 2011
    ..Average RGD spacing similar to that found in fibronectin leads to lipid raft accumulation at FAs, enhances sensitivity to VEGF stimulation, and controls migration in endothelial cells...
  15. pmc Cyclosporin A decreases apolipoprotein E secretion from human macrophages via a protein phosphatase 2B-dependent and ATP-binding cassette transporter A1 (ABCA1)-independent pathway
    Maaike Kockx
    Centre for Vascular Research, School of Medical Sciences, University of New South Wales, Sydney, New South Wales 2052, Australia
    J Biol Chem 284:24144-54. 2009
    ..Taken together, these results establish a novel mechanism for the pro-atherosclerotic effects of CsA, and establish for the first time a role for PP2B in regulating the intracellular transport and secretion of apoE...
  16. pmc Functional implications of plasma membrane condensation for T cell activation
    Carles Rentero
    Centre for Vascular Research, University of New South Wales and the Department of Haematology, Prince of Wales Hospital, Sydney, Australia
    PLoS ONE 3:e2262. 2008
    ..Our data emphasizes lipids as an important medium for the organization at T cell activation sites and strongly indicates that membrane condensation is an important element of the T cell activation process...
  17. ncbi request reprint Apolipoprotein A-1 interaction with plasma membrane lipid rafts controls cholesterol export from macrophages
    Katharina Gaus
    Macrophage Biology Group, Centre for Vascular Research, School of Medical Sciences, University of New South Wales, Sydney, NSW, Australia
    FASEB J 18:574-6. 2004
    ..We propose that cholesterol exported to apoA-1 from this major slow efflux pool derives from nonraft regions of the plasma membrane but that the interaction of apoA-1 with lipid rafts is necessary to stimulate this efflux...
  18. doi request reprint Phagocytosis of IgG-coated polystyrene beads by macrophages induces and requires high membrane order
    Astrid Magenau
    Centre for Vascular Research, University of New South Wales, Sydney, Australia
    Traffic 12:1730-43. 2011
    ..In conclusion, our results suggest that ordered membranes function to co-ordinate F-actin remodelling and that the biophysical properties of phagosomal membranes are essential for phagocytosis...
  19. pmc Characterization of a new series of fluorescent probes for imaging membrane order
    Joanna M Kwiatek
    Centre for Vascular Research and Australian Centre for NanoMedicine, University of New South Wales, Sydney, Australia
    PLoS ONE 8:e52960. 2013
    ..These probes expand the current palate of lipid order-sensing fluorophores affording greater flexibility in the excitation/emission wavelengths and possibly new opportunities in membrane biology...
  20. ncbi request reprint Si-C linked oligo(ethylene glycol) layers in silicon-based photonic crystals: optimization for implantable optical materials
    Kristopher A Kilian
    School of Chemistry, University of New South Wales, Sydney, NSW 2052, Australia
    Biomaterials 28:3055-62. 2007
    ..The antifouling behaviour and greater stability to physiological conditions provided by this chemistry enhances the suitability of porous silicon for biomaterials applications...
  21. doi request reprint Different functionalization of the internal and external surfaces in mesoporous materials for biosensing applications using "click" chemistry
    Bin Guan
    School of Chemistry, The University of New South Wales, Sydney, NSW 2052, Australia
    Langmuir 27:328-34. 2011
    ..Results presented here demonstrate a general approach to selectively modify mesoporous silicon samples with potential applications for cell-based biosensing...
  22. ncbi request reprint Peptide-modified optical filters for detecting protease activity
    Kristopher A Kilian
    School of Chemistry, Centre for Vascular Research, University of New South Wales, Sydney NSW 2052, Australia
    ACS Nano 1:355-61. 2007
    ..The lowest detected concentration of enzyme was 37 nM (7.4 pmol in 200 microL)...
  23. doi request reprint Sub-resolution lipid domains exist in the plasma membrane and regulate protein diffusion and distribution
    Dylan M Owen
    Centre for Vascular Research and Australian Centre for NanoMedicine, University of New South Wales, High Street, Sydney, New South Wales 2052, Australia
    Nat Commun 3:1256. 2012
    ..Our data therefore propose a membrane model in which the majority of the plasma membrane is covered by cholesterol-dependent, ordered lipid domains that contribute to the non-random distribution and diffusion of membrane constituents...
  24. doi request reprint Optical techniques for imaging membrane domains in live cells (live-cell palm of protein clustering)
    Dylan M Owen
    Centre for Vascular Research, University of New South Wales, Sydney, Australia
    Methods Enzymol 504:221-35. 2012
    ..J., Owen, D. M., Rossy, J., Magenau, A., Wehrmann, M., Gooding, J. J. , and Gaus, K. (2011). Pre-existing clusters of the adaptor Lat do not participate in early T cell signaling events. Nat. Immunol.12, 655-662.]...
  25. ncbi request reprint S100A8 and S100A9 in human arterial wall. Implications for atherogenesis
    Michelle M McCormick
    School of Medical Sciences, St Vincent s Hospital, University of New South Wales, Sydney, Australia
    J Biol Chem 280:41521-9. 2005
    ..S100A8 and S100A9 in atherosclerotic plaque and calcifying matrix vesicles may significantly influence redox- and Ca2+-dependent processes during atherogenesis and its chronic complications, particularly dystrophic calcification...
  26. doi request reprint Pre-existing clusters of the adaptor Lat do not participate in early T cell signaling events
    David J Williamson
    Centre for Vascular Research, University of New South Wales, Sydney, Australia
    Nat Immunol 12:655-62. 2011
    ..Our data suggest that TCR ligation preconditions the membrane for vesicle recruitment and bulk activation of the Lat signaling network...
  27. doi request reprint Smart tissue culture: in situ monitoring of the activity of protease enzymes secreted from live cells using nanostructured photonic crystals
    Kristopher A Kilian
    School of Chemistry, University of New South Wales, Sydney, New South Wales 2052, Australia
    Nano Lett 9:2021-5. 2009
    ..In situ monitoring is demonstrated by detecting secretion of matrix metalloprotease 9 from stimulated human macrophages...
  28. ncbi request reprint Apolipoprotein A-I-stimulated apolipoprotein E secretion from human macrophages is independent of cholesterol efflux
    Maaike Kockx
    Macrophage Biology Group, Centre for Vascular Research, University of New South Wales, Sydney 2052, Australia
    J Biol Chem 279:25966-77. 2004
    ....
  29. doi request reprint Introducing distinctly different chemical functionalities onto the internal and external surfaces of mesoporous materials
    Kristopher A Kilian
    School of Chemistry, University of New South Wales, Sydney 2052, Australia
    Angew Chem Int Ed Engl 47:2697-9. 2008
  30. doi request reprint The lipid raft hypothesis revisited--new insights on raft composition and function from super-resolution fluorescence microscopy
    Dylan M Owen
    Centre for Vascular Research and Australian Centre for NanoMedicine, University of New South Wales, Sydney, Australia
    Bioessays 34:739-47. 2012
    ..This review describes such techniques, particularly as applied to the study of membrane organisation, synthesising newly emerging facets of lipid raft biology into a state-of-the art model...
  31. doi request reprint Electrochemical "switching" of Si(100) modular assemblies
    Simone Ciampi
    School of Chemistry, The University of New South Wales, Sydney, NSW 2052, Australia
    J Am Chem Soc 134:844-7. 2012
    ..The electrochemical conversion of the modified electrode surface is demonstrated by X-ray photoelectron spectroscopy, X-ray reflectometry, contact angle and cell adhesion studies...
  32. pmc The relative importance of topography and RGD ligand density for endothelial cell adhesion
    Guillaume Le Saux
    School of Chemistry, University of New South Wales, Sydney, New South Wales, Australia
    PLoS ONE 6:e21869. 2011
    ....
  33. ncbi request reprint Mesoporous silicon photonic crystal microparticles: towards single-cell optical biosensors
    Bin Guan
    School of Chemistry, The University of New South Wales, Sydney, NSW, Australia
    Faraday Discuss 149:301-17; discussion 333-56. 2011
    ..The particles are shown to remain stable in physiological buffers and human blood for longer than one week. Finally, the modification of the PSi particles with functional antibodies is achieved...
  34. ncbi request reprint Secretion of apolipoprotein E from macrophages occurs via a protein kinase A and calcium-dependent pathway along the microtubule network
    Maaike Kockx
    Macrophage Biology Group, Centre for Vascular Research, School of Medical Sciences, University of New South Wales, Australia
    Circ Res 101:607-16. 2007
    ..We conclude that macrophage secretion of apoE occurs via a PKA- and calcium-dependent pathway along the microtubule network...
  35. ncbi request reprint Optimized time-gated generalized polarization imaging of Laurdan and di-4-ANEPPDHQ for membrane order image contrast enhancement
    Dylan M Owen
    Centre for Vascular Research, University of New South Wales, Sydney, Australia
    Microsc Res Tech 73:618-22. 2010
    ..This improvement in image contrast enhancement will be invaluable when studying the role of lipid rafts in fixed and live cell systems...
  36. doi request reprint Quantitative imaging of membrane lipid order in cells and organisms
    Dylan M Owen
    Centre for Vascular Research, University of New South Wales, Sydney, Australia
    Nat Protoc 7:24-35. 2012
    ..This approach, which takes around 4 h, directly examines the organization of cellular membranes and is distinct from alternative approaches that infer membrane order by measuring probe partitioning or dynamics...
  37. ncbi request reprint Quantitative microscopy: protein dynamics and membrane organisation
    Dylan M Owen
    Centre for Vascular Research, University of New South Wales, and the Department of Haematology, Prince of Wales Hospital, Sydney, Australia
    Traffic 10:962-71. 2009
    ..New multi-dimensional microscopy approaches may afford greater temporal and spatial resolution resulting in more accurate quantification of protein and membrane dynamics in live cells...
  38. pmc Imaging membrane lipid order in whole, living vertebrate organisms
    Dylan M Owen
    Center for Vascular Research, University of New South Wales, Sydney, Australia
    Biophys J 99:L7-9. 2010
    ..The transition of membrane order imaging from cultured cell lines to living organisms is an important step forward in understanding the physiological relevance of membrane microdomains including lipid rafts...
  39. doi request reprint Conformational states of the kinase Lck regulate clustering in early T cell signaling
    Jérémie Rossy
    Centre for Vascular Research and Australian Centre for NanoMedicine, University of New South Wales, Sydney, Australia
    Nat Immunol 14:82-9. 2013
    ..Our data suggest that Lck conformational states represent an intrinsic mechanism for the intermolecular organization of early T cell signaling...
  40. doi request reprint Using an electrical potential to reversibly switch surfaces between two states for dynamically controlling cell adhesion
    Cheuk Chi Albert Ng
    School of Chemistry and Australian Centre for NanoMedicine, The University of New South Wales, Sydney NSW 2052, Australia
    Angew Chem Int Ed Engl 51:7706-10. 2012
    ..By applying potentials of +300 or -300 mV, the surfaces could be dynamically switched to make the peptide accessible or inaccessible to cells...
  41. ncbi request reprint Roles of ATP binding cassette transporters A1 and G1, scavenger receptor BI and membrane lipid domains in cholesterol export from macrophages
    Wendy Jessup
    Centre for Vascular Research, at the School of Medical Sciences, University of New South Wales, Sydney, Australia
    Curr Opin Lipidol 17:247-57. 2006
    ..The review discusses recent developments in our understanding of how these steps occur, with particular emphasis on the macrophage, the major site of cellular cholesterol accumulation in atherosclerosis...
  42. pmc Dynamic organization of lymphocyte plasma membrane: lessons from advanced imaging methods
    Dylan M Owen
    Centre for Vascular Research, University of New South Wales, Sydney, NSW, Australia
    Immunology 131:1-8. 2010
    ..We provide a comprehensive up-to-date view on the existence of membrane lipid and protein clusters such as lipid rafts and suggest research directions to better understand these highly dynamic entities on the surface of immune cells...
  43. doi request reprint Substrate independent assembly of optical structures guided by biomolecular interactions
    Till Böcking
    School of Physics, School of Chemistry, and Centre for Vascular Research, University of New South Wales, Sydney, NSW 2052, Australia
    ACS Appl Mater Interfaces 2:3270-5. 2010
    ..The key features of this new technology include substrate independent assembly, cross-platform vertical scale integration, and selective registration of components based on complementary biomolecular interactions...
  44. pmc Quantitative analysis of three-dimensional fluorescence localization microscopy data
    Dylan M Owen
    Centre for Vascular Research and Australian Centre for NanoMedicine, University of New South Wales, Sydney, Australia
    Biophys J 105:L05-7. 2013
    ..This approach is particularly useful to map the topography of the plasma membrane and measure protein distribution within an undulating membrane...
  45. doi request reprint VAMP7 controls T cell activation by regulating the recruitment and phosphorylation of vesicular Lat at TCR-activation sites
    Paola Larghi
    Institut Curie, Centre de Recherche, Pavillon Pasteur, Paris, France
    Nat Immunol 14:723-31. 2013
    ..Our findings suggest that the transport and docking of Lat-containing vesicles with target membranes containing TCRs regulates TCR-induced signaling...
  46. doi request reprint Annexin A6 is an organizer of membrane microdomains to regulate receptor localization and signalling
    Rhea Cornely
    Centre for Vascular Research, University of New South Wales, Sydney, Australia
    IUBMB Life 63:1009-17. 2011
    ....
  47. ncbi request reprint Versatile "click chemistry" approach to functionalizing silicon quantum dots: applications toward fluorescent cellular imaging
    Xiaoyu Cheng
    School of Chemistry, Australian Centre for Nanomedicine, Centre for Vascular Research, and School of Physics, University of New South Wales, Sydney, New South Wales 2052, Australia
    Langmuir 30:5209-16. 2014
    ..In addition, the SiQDs were shown to be non-cytotoxic at concentrations up to 240 μg/mL. The results presented herein provide good evidence for the versatility of functionalized SiQDs for fluorescent bioimaging application. ..
  48. doi request reprint Electrochemical behavior of gold colloidal alkyl modified silicon surfaces
    Guillaume Le Saux
    School of Chemistry and Centre for Vascular Research, The University of New South Wales, Sydney, New South Wales 2052, Australia
    ACS Appl Mater Interfaces 1:2477-83. 2009
    ..Cyclic voltammetry showed that the enhancement of electron transfer to the silicon surface by the presence of nanoparticles is influenced by the number of tethered nanoparticles...
  49. ncbi request reprint Single-step DNA immobilization on antifouling self-assembled monolayers covalently bound to silicon (111)
    Till Böcking
    School of Chemistry, University of New South Wales, Sydney, NSW 2052, Australia
    Langmuir 22:3494-6. 2006
    ..Immobilization of thiolated DNA was achieved in a single step to fabricate biorecognition interfaces showing the hybridization of complementary DNA at low concentrations and negligible binding of noncomplementary DNA...
  50. doi request reprint Actin dynamics drive membrane reorganization and scission in clathrin-independent endocytosis
    Winfried Römer
    Institut Curie, Centre de Recherche, CNRS UMR, Paris, France
    Cell 140:540-53. 2010
    ....
  51. doi request reprint Super-resolution imaging by localization microscopy
    Dylan M Owen
    Lowy Cancer Research Centre, Centre for Vascular Research, University of New South Wales, Sydney, Australia
    Methods Mol Biol 950:81-93. 2013
    ..In this example, the system under investigation will be fixed HeLa cells transfected with the photo-switchable fluorescent protein PS-CFP2 targeted to the plasma membrane by fusion to the N-terminus of the protein kinase Lck...
  52. doi request reprint Expression and stability of two isoforms of ABCG1 in human vascular cells
    Ingrid C Gelissen
    Centre for Vascular Research, University of New South Wales, Sydney, Australia
    Atherosclerosis 208:75-82. 2010
    ....
  53. ncbi request reprint LILRA5 is expressed by synovial tissue macrophages in rheumatoid arthritis, selectively induces pro-inflammatory cytokines and IL-10 and is regulated by TNF-alpha, IL-10 and IFN-gamma
    Ainslie Mitchell
    Centre for Infection and Inflammation Research, School of Medical Sciences, University of New South Wales, Sydney, NSW, Australia
    Eur J Immunol 38:3459-73. 2008
    ..Increased expression of LILRA5 in rheumatoid tissue, together with its ability to induce key cytokines involved in RA, suggests that this novel receptor may contribute to disease pathogenesis...
  54. doi request reprint Do signalling endosomes play a role in T cell activation?
    Carola Benzing
    Centre for Vascular Research and Australian Centre for NanoMedicine, University of New South Wales, Sydney, Australia
    FEBS J 280:5164-76. 2013
    ..We review the various mechanisms by which endosomes contribute to signalling pathways and consider whether signalling endosomes play a role in T cell signalling...
  55. doi request reprint Insights into adhesion biology using single-molecule localization microscopy
    Thibault Tabarin
    Centre for Vascular Research, University of the New South Wales, Sydney, 2052 Australia
    Chemphyschem 15:606-18. 2014
    ..With current improvements in SMLM instrumentation and analysis, it has become possible to study cellular adhesions at the single-molecule level. ..
  56. ncbi request reprint Method for co-cluster analysis in multichannel single-molecule localisation data
    Jérémie Rossy
    Centre for Vascular Research and Australian Centre for NanoMedicine, University of New South Wales, Sydney, Australia
    Histochem Cell Biol 141:605-12. 2014
    ..Analysing co-clustering in this manner is generalizable to higher numbers of fluorescent species and to three-dimensional or live cell data sets. ..
  57. doi request reprint Super-resolution microscopy of the immunological synapse
    Jérémie Rossy
    Centre for Vascular Research, University of New South Wales, Sydney, Australia
    Curr Opin Immunol 25:307-12. 2013
    ..Here, we review the advantages and potential of super-resolution microscopy for elucidating the regulation of many aspects of immune signalling. ..
  58. ncbi request reprint Oxidized lipoproteins and macrophages
    Wendy Jessup
    Cell Biology Group, Heart Research Institute, 145 Missenden Road, Camperdown, Sydney, NSW 2050, Australia
    Vascul Pharmacol 38:239-48. 2002
    ..Here we review macrophage responses to oxidized lipoproteins and provide novel data on the effects of a major oxidation product, 7-ketocholesterol, on high-density lipoprotein (HDL) function in cholesterol removal from macrophages...
  59. pmc Fluctuation-based imaging of nuclear Rac1 activation by protein oligomerisation
    Elizabeth Hinde
    1 Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, USA 2 Centre for Vascular Research and Australian Centre for NanoMedicine, University of New South Wales, Sydney, Australia
    Sci Rep 4:4219. 2014
    ..Only with this multiplexed approach can we assess the oligomeric state a molecular complex must form in order to regulate a complex signalling network. ..
  60. ncbi request reprint Formation of tetra(ethylene oxide) terminated Si-C linked monolayers and their derivatization with glycine: an example of a generic strategy for the immobilization of biomolecules on silicon
    Till Böcking
    School of Physics and School of Chemistry, University of New South Wales, Sydney, Australia
    Langmuir 21:10522-9. 2005
    ....
  61. ncbi request reprint Agrin elicits membrane lipid condensation at sites of acetylcholine receptor clusters in C2C12 myotubes
    Françoise Stetzkowski-Marden
    Biologie Cellulaire des Membranes, Institut Jacques Monod, Unite Mixte de Recherche 7592, Centre National de la Recherche Scientifique, Universite Paris 6, Universite Paris 7, F 75251 Paris Cedex 05, France
    J Lipid Res 47:2121-33. 2006
    ..These data led us to propose a model in which lipid rafts play a pivotal role in the assembly of the postsynaptic membrane at the neuromuscular junction upon agrin signaling...
  62. pmc The raft-promoting property of virion-associated cholesterol, but not the presence of virion-associated Brij 98 rafts, is a determinant of human immunodeficiency virus type 1 infectivity
    Shahan Campbell
    Macfarlane Burnet Institute for Medical Research and Public Health, Cnr Punt and Commercial Roads, Melbourne 3004, Victoria, Australia
    J Virol 78:10556-65. 2004
    ..Our data provide the first direct assessment of virion-associated Brij 98 rafts in retroviral replication and illustrate the importance of the raft-promoting property of virion-associated cholesterol in HIV-1 replication...
  63. ncbi request reprint Cyclodextrins differentially mobilize free and esterified cholesterol from primary human foam cell macrophages
    Sue M Liu
    Heart Research Institute, Camperdown, Sydney New South Wales, Australia
    J Lipid Res 44:1156-66. 2003
    ..It is concluded that differential depletion of plasma membrane cholesterol pools regulates cholesterol efflux and CE clearance in human macrophages...
  64. ncbi request reprint Plasma membrane segregation during T cell activation: probing the order of domains
    Thomas Harder
    Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom
    Curr Opin Immunol 19:470-5. 2007
    ....
  65. pmc Annexin A6-induced alterations in cholesterol transport and caveolin export from the Golgi complex
    Laia Cubells
    Departament de Biologia Cellular, Facultat de Medicina, Universitat de Barcelona, Casanova 143, 08036 Barcelona, Spain
    Traffic 8:1568-89. 2007
    ..In summary, this study demonstrates that elevated expression levels of AnxA6 perturb the intracellular distribution of cholesterol, which indirectly inhibits the exit of caveolin from the Golgi complex...
  66. ncbi request reprint The constrained amino acid beta-Acc confers potency and selectivity to integrin ligands
    Sylwia Urman
    Fakultat fur Chemie, Organische und Bioorganische Chemie, Universitat Bielefeld, Universitatsstrasse 25, 33615 Bielefeld, Germany
    Angew Chem Int Ed Engl 46:3976-8. 2007
  67. pmc Myelin basic protein-dependent plasma membrane reorganization in the formation of myelin
    Dirk Fitzner
    Centre for Biochemistry and Molecular Cell Biology, University of Gottingen, Gottingen, Germany
    EMBO J 25:5037-48. 2006
    ..We propose that this mechanism is essential for myelin to perform its insulating function during nerve conduction...
  68. ncbi request reprint Short peptide receptor mimics for atherosclerosis risk assessment of LDL
    Katharina Gaus
    Institute of Biotechnology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QT, UK
    Biosens Bioelectron 18:151-64. 2003
    ..Simple correlation of this form could provide good tests for atherosclerosis risk...
  69. ncbi request reprint Shiga toxin induces tubular membrane invaginations for its uptake into cells
    Winfried Römer
    Institut Curie, Centre de Recherche, Laboratoire Trafic, Signalisation et Ciblage Intracellulaires, Paris Cedex 05, France
    Nature 450:670-5. 2007
    ..The physical principles underlying this basic cargo-induced membrane uptake may also be relevant to other internalization processes, creating a rationale for conceptualizing the perplexing diversity of endocytic routes...
  70. pmc Plasma membrane polarization during mating in yeast cells
    Tomasz J Proszynski
    Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany
    J Cell Biol 173:861-6. 2006
    ..Furthermore, we provide evidence that the lipid bilayer at the mating projection is more condensed than the plasma membrane enclosing the cell body, and that sphingolipids are required for this lipid organization...
  71. pmc FAPP2, cilium formation, and compartmentalization of the apical membrane in polarized Madin-Darby canine kidney (MDCK) cells
    Otilia V Vieira
    Max Planck Institute for Molecular Cell Biology and Genetics, D 01307 Dresden, Germany
    Proc Natl Acad Sci U S A 103:18556-61. 2006
    ..These results indicate that the compartmentalization of the apical membrane in MDCK cells into the ciliary membrane and the surrounding membrane depends on the balance of raft and nonraft domains...