- Witham K, Minchin R, Butcher N. Role for human arylamine N-acetyltransferase 1 in the methionine salvage pathway. Biochem Pharmacol. 2017;125:93-100 pubmed publisher..The present study has identified a novel endogenous role for human NAT1 that might explain some of its effects in cancer cell growth and survival. ..
- Butcher N, Arulpragasam A, Minchin R. Proteasomal degradation of N-acetyltransferase 1 is prevented by acetylation of the active site cysteine: a mechanism for the slow acetylator phenotype and substrate-dependent down-regulation. J Biol Chem. 2004;279:22131-7 pubmed..From this study, we conclude that NAT1 exists in the cell in either a stable acetylated state or an unstable non-acetylated state and that mutations in the NAT1 gene that prevent protein acetylation produce a slow acetylator phenotype. ..
- Butcher N, Minchin R. Arylamine N-acetyltransferase 1 gene regulation by androgens requires a conserved heat shock element for heat shock factor-1. Carcinogenesis. 2010;31:820-6 pubmed publisher..Our results show that HSF1 is an important transcription factor for induction of NAT1 in human cells and is required for androgen activation of the NAT1 promoter. ..