R C Baxter

Summary

Affiliation: University of Sydney
Country: Australia

Publications

  1. pmc Signalling pathways involved in antiproliferative effects of IGFBP-3: a review
    R C Baxter
    Kolling Institute of Medical Research, University of Sydney, Royal North Shore Hospital, Sydney, NSW 2065, Australia
    Mol Pathol 54:145-8. 2001
  2. doi request reprint Actions of IGF binding proteins and related proteins in adipose tissue
    Robert C Baxter
    Kolling Institute of Medical Research, University of Sydney, Royal North Shore Hospital, St Leonards NSW 2065, Australia
    Trends Endocrinol Metab 20:499-505. 2009
  3. ncbi request reprint Molecular distribution of IGF binding protein-5 in human serum
    Robert C Baxter
    Kolling Institute of Medical Research, University of Sydney, Royal North Shore Hospital, St Leonards, New South Wales 2065, Australia
    J Clin Endocrinol Metab 87:271-6. 2002
  4. ncbi request reprint Measurement of the acid-labile subunit of the insulin-like growth factor binding protein complex in human serum: a comparison of four immunoassays
    R C Baxter
    Kolling Institute of Medical Research, University of Sydney, Royal North Shore Hospital, Sydney, New South Wales 2065, Australia
    J Endocrinol 165:271-9. 2000
  5. ncbi request reprint Inhibition of the insulin-like growth factor (IGF)-IGF-binding protein interaction
    R C Baxter
    Kolling Institute of Medical Research, University of Sydney, Royal North Shore Hospital, St Leonards, NSW, Australia
    Horm Res 55:68-72. 2001
  6. ncbi request reprint Insulin-like growth factor (IGF)-binding proteins: interactions with IGFs and intrinsic bioactivities
    R C Baxter
    Kolling Institute of Medical Research, University of Sydney, Royal North Shore Hospital, Sydney, New South Wales 2065, Australia
    Am J Physiol Endocrinol Metab 278:E967-76. 2000
  7. ncbi request reprint Insulin-like growth factor (IGF)-binding protein 5 forms an alternative ternary complex with IGFs and the acid-labile subunit
    S M Twigg
    Kolling Institute of Medical Research, University of Sydney, Royal North Shore Hospital, St Leonards, New South Wales 2065, Australia
    J Biol Chem 273:6074-9. 1998
  8. ncbi request reprint Growth inhibition by insulin-like growth factor-binding protein-3 in T47D breast cancer cells requires transforming growth factor-beta (TGF-beta ) and the type II TGF-beta receptor
    S Fanayan
    Kolling Institute of Medical Research, University of Sydney, Royal North Shore Hospital, St Leonards, New South Wales 2065, Australia
    J Biol Chem 275:39146-51. 2000
  9. ncbi request reprint Adenoviral-mediated expression of human insulin-like growth factor-binding protein-3
    S M Firth
    Kolling Institute of Medical Research, Royal North Shore Hospital, St Leonards, NSW, Australia
    Protein Expr Purif 16:202-11. 1999
  10. ncbi request reprint Purification and characterization of the acid-labile subunit of rat serum insulin-like growth factor binding protein complex
    R C Baxter
    Department of Endocrinology, Royal Prince Alfred Hospital, Sydney, New South Wales, Australia
    Endocrinology 134:848-52. 1994

Collaborators

Detail Information

Publications90

  1. pmc Signalling pathways involved in antiproliferative effects of IGFBP-3: a review
    R C Baxter
    Kolling Institute of Medical Research, University of Sydney, Royal North Shore Hospital, Sydney, NSW 2065, Australia
    Mol Pathol 54:145-8. 2001
    ..These recent findings all point to a complex intracellular mode of action of IGFBP-3, which will need to be better understood if anti-cancer treatments are to take advantage of the antiproliferative activity of IGFBP-3...
  2. doi request reprint Actions of IGF binding proteins and related proteins in adipose tissue
    Robert C Baxter
    Kolling Institute of Medical Research, University of Sydney, Royal North Shore Hospital, St Leonards NSW 2065, Australia
    Trends Endocrinol Metab 20:499-505. 2009
    ..This review examines the current knowledge of IGFBP regulation and actions in adipocytes and proposes a common regulatory pathway involving IGFBP-3 and CTGF/CCN2...
  3. ncbi request reprint Molecular distribution of IGF binding protein-5 in human serum
    Robert C Baxter
    Kolling Institute of Medical Research, University of Sydney, Royal North Shore Hospital, St Leonards, New South Wales 2065, Australia
    J Clin Endocrinol Metab 87:271-6. 2002
    ..As free or binary complexed IGFBP-5 is a relatively high proportion of the total, we speculate that, alone or as a carrier of IGFs, IGFBP-5 might have preferential access to the tissues, where it could act to stimulate growth...
  4. ncbi request reprint Measurement of the acid-labile subunit of the insulin-like growth factor binding protein complex in human serum: a comparison of four immunoassays
    R C Baxter
    Kolling Institute of Medical Research, University of Sydney, Royal North Shore Hospital, Sydney, New South Wales 2065, Australia
    J Endocrinol 165:271-9. 2000
    ..We conclude that a variety of assay formats and reagents can yield serum ALS values of potential clinical utility...
  5. ncbi request reprint Inhibition of the insulin-like growth factor (IGF)-IGF-binding protein interaction
    R C Baxter
    Kolling Institute of Medical Research, University of Sydney, Royal North Shore Hospital, St Leonards, NSW, Australia
    Horm Res 55:68-72. 2001
    ..IGFBPs can interfere in IGF analyses, so removal of IGFBPs, and minimization of their influence in IGF assays, is essential to ensure valid quantification of IGFs...
  6. ncbi request reprint Insulin-like growth factor (IGF)-binding proteins: interactions with IGFs and intrinsic bioactivities
    R C Baxter
    Kolling Institute of Medical Research, University of Sydney, Royal North Shore Hospital, Sydney, New South Wales 2065, Australia
    Am J Physiol Endocrinol Metab 278:E967-76. 2000
    ..The regulation of cell sensitivity to inhibitory IGFBP signaling may play a role in the growth control of malignant cells...
  7. ncbi request reprint Insulin-like growth factor (IGF)-binding protein 5 forms an alternative ternary complex with IGFs and the acid-labile subunit
    S M Twigg
    Kolling Institute of Medical Research, University of Sydney, Royal North Shore Hospital, St Leonards, New South Wales 2065, Australia
    J Biol Chem 273:6074-9. 1998
    ..The demonstration of IGFBP-5-containing ternary complexes indicates an unrecognized form of IGF transport in the circulation and an additional mechanism for regulating IGF bioavailability...
  8. ncbi request reprint Growth inhibition by insulin-like growth factor-binding protein-3 in T47D breast cancer cells requires transforming growth factor-beta (TGF-beta ) and the type II TGF-beta receptor
    S Fanayan
    Kolling Institute of Medical Research, University of Sydney, Royal North Shore Hospital, St Leonards, New South Wales 2065, Australia
    J Biol Chem 275:39146-51. 2000
    ..These data suggest that IGFBP-3 inhibitory signaling requires an active TGF-beta signaling pathway and implicate Smad2 and Smad3 in IGFBP-3 signal transduction...
  9. ncbi request reprint Adenoviral-mediated expression of human insulin-like growth factor-binding protein-3
    S M Firth
    Kolling Institute of Medical Research, Royal North Shore Hospital, St Leonards, NSW, Australia
    Protein Expr Purif 16:202-11. 1999
    ..IGFBP-3(Ad) also exhibited increased binding to T47D cells which may be related to the glycosylation state of the protein...
  10. ncbi request reprint Purification and characterization of the acid-labile subunit of rat serum insulin-like growth factor binding protein complex
    R C Baxter
    Department of Endocrinology, Royal Prince Alfred Hospital, Sydney, New South Wales, Australia
    Endocrinology 134:848-52. 1994
    ..GH-deficient rats showed 60-75% lower values than controls. This study shows rat ALS to have similar binding properties, and age and GH dependence, to human ALS. The new RIA will facilitate studies of IGF and IGFBP regulation in the rat...
  11. ncbi request reprint Structural determinants of ligand and cell surface binding of insulin-like growth factor-binding protein-3
    S M Firth
    Kolling Institute of Medical Research, University of Sydney, Royal North Shore Hospital, St Leonards, New South Wales 2065, Australia
    J Biol Chem 273:2631-8. 1998
    ..We conclude that residues 228-232 of IGFBP-3 are essential for cell association and are required for normal ALS binding affinity...
  12. ncbi request reprint The effect of phosphorylation by casein kinase 2 on the activity of insulin-like growth factor-binding protein-3
    J A Coverley
    Kolling Institute of Medical Research, University of Sydney, Royal North Shore Hospital, St Leonards, New South Wales, Australia
    Endocrinology 141:564-70. 2000
    ..Relative resistance to proteolysis and poor binding to cells suggest that CK2-phospho-IGFBP-3 may be a significant inhibitor of IGF activity in the extracellular environment...
  13. ncbi request reprint Changes in the circulating IGF system during short-term fasting and refeeding in rats
    J Frystyk
    Kolling Institute of Medical Research, Royal North Shore Hospital, University of Sydney, Sydney, New South Wales 2065, Australia
    Am J Physiol 277:E245-52. 1999
    ..Finally, the regulation of the hepatic synthesis of IGF-I, IGFBP-1, and ALS seems to differ substantially...
  14. ncbi request reprint Insulin-like growth factor-binding protein (IGFBP)-3 and IGFBP-5 share a common nuclear transport pathway in T47D human breast carcinoma cells
    L J Schedlich
    Kolling Institute of Medical Research, University of Sydney, Royal North Shore Hospital, Sydney, New South Wales 2065, Australia
    J Biol Chem 273:18347-52. 1998
    ....
  15. ncbi request reprint Erythropoietin administration does not influence the GH--IGF axis or makers of bone turnover in recreational athletes
    A E Nelson
    Garvan Institute of Medical Research and Department of Endocrinology, Darlinghurst, NSW, Australia
    Clin Endocrinol (Oxf) 63:305-9. 2005
    ..The aim was to determine whether r-HuEPO induces similar effects on the IGF-system and collagen turnover in healthy athletes...
  16. ncbi request reprint Characterisation of recombinant glycosylation variants of insulin-like growth factor binding protein-3
    S M Firth
    Kolling Institute of Medical Research, Royal North Shore Hospital, St Leonards, New South Wales 2065, Australia
    J Endocrinol 160:379-87. 1999
    ..Therefore, while the carbohydrate units appear to be non-essential to ALS or IGF binding, they may modulate other biological activities of IGFBP-3...
  17. ncbi request reprint Phosphorylation of insulin-like growth factor binding proteins
    J A Coverley
    Kolling Institute of Medical Research, University of Sydney, Royal North Shore Hospital, St Leonards, NSW, Australia
    Mol Cell Endocrinol 128:1-5. 1997
    ..Although no specific functions have yet been ascribed to phosphorylated forms of the other IGFBPs, current evidence supports the proposal that IGFBP phosphorylation plays an important role in the regulation of IGFBP function...
  18. ncbi request reprint Acid-labile subunit regulation during the early stages of liver regeneration: implications for glucoregulation
    P J Delhanty
    Department of Molecular Medicine, University of Sydney, Kolling Institute of Medical Research, St Leonards, New South Wales 2065, Australia
    Am J Physiol Endocrinol Metab 280:E287-95. 2001
    ..Suppressed circulating ALS may alter insulin-like growth factor bioavailability and constitute a mechanism to maintain relatively normal glucoregulation after loss of liver mass...
  19. ncbi request reprint A purified bovine serum albumin preparation contains an insulin-like growth factor (IGF) binding protein-3 fragment that forms ternary complexes selectively with IGF-II and the acid-labile subunit
    S M Twigg
    Kolling Institute of Medical Research, University of Sydney, Royal North Shore Hospital, Pacific Highway, St Leonards, Sydney, NSW 2065, Australia
    Growth Horm IGF Res 10:215-23. 2000
    ..In contrast to intact human IGFBP-3 and IGFBP-5, this form of bovine IGFBP-3 forms ternary complexes preferentially with IGF-II rather than IGF-I...
  20. ncbi request reprint Development of resistance to insulin-like growth factor binding protein-3 in transfected T47D breast cancer cells
    S M Firth
    Kolling Institute of Medical Research, University of Sydney, New South Wales, Australia
    Biochem Biophys Res Commun 246:325-9. 1998
    ..We hypothesize that development of resistance to the antiproliferative action of IGFBP-3 might be an important step in the malignant progression of breast cancer cells...
  21. pmc Structure of the Mr 140,000 growth hormone-dependent insulin-like growth factor binding protein complex: determination by reconstitution and affinity-labeling
    R C Baxter
    Department of Endocrinology, Royal Prince Alfred Hospital, Sydney, NSW, Australia
    Proc Natl Acad Sci U S A 86:6898-902. 1989
    ..We conclude that the growth hormone-dependent Mr 140,000 IGF-binding protein complex in human serum has three components: the alpha (acid-labile) subunit, the beta (binding) subunit, and the gamma (growth factor) subunit...
  22. ncbi request reprint Cloning and characterization of the rat gene for the acid-labile subunit of the insulin-like growth factor binding protein complex
    P J Delhanty
    Kolling Institute of Medical Research, University of Sydney, Royal North Shore Hospital, St Leonards, NSW, Australia
    J Mol Endocrinol 19:267-77. 1997
    ..Our data demonstrate the uncomplicated structure of the rat ALS gene, and the promoter function and presence of potential regulatory elements in the region upstream of the protein-coding sequence...
  23. ncbi request reprint The IGF axis and programmed cell death
    A J Butt
    Kolling Institute of Medical Research, University of Sydney, Royal North Shore Hospital, St Leonards, New South Wales, Australia
    Immunol Cell Biol 77:256-62. 1999
    ..This review summarizes what is currently known about the role of the IGF system in the regulation of apoptosis, highlighting its implications in the context of tumorigenesis...
  24. ncbi request reprint Insulin-like growth factor-binding protein 5 complexes with the acid-labile subunit. Role of the carboxyl-terminal domain
    S M Twigg
    Kolling Institute of Medical Research, University of Sydney, Royal North Shore Hospital, St Leonards, New South Wales 2065, Australia
    J Biol Chem 273:28791-8. 1998
    ..These findings are consistent with the basic carboxyl-terminal domain on IGFBP-5 being the principal site in IGFBP-5 that binds to ALS...
  25. doi request reprint The role of insulin-like growth factor binding protein-3 in the breast cancer cell response to DNA-damaging agents
    M Z Lin
    Hormones and Cancer Division, Kolling Institute of Medical Research, University of Sydney, Royal North Shore Hospital, St Leonards, Australia
    Oncogene 33:85-96. 2014
    ..This suggests the possibility of a therapeutic approach for sensitizing breast cancer to chemo- or radiotherapy by targeting the DNA repair function of IGFBP-3. ..
  26. ncbi request reprint Insulin-like growth factor-binding protein-3 modulates expression of Bax and Bcl-2 and potentiates p53-independent radiation-induced apoptosis in human breast cancer cells
    A J Butt
    Kolling Institute of Medical Research, University of Sydney, Royal North Shore Hospital, New South Wales 2065, Australia
    J Biol Chem 275:39174-81. 2000
    ....
  27. ncbi request reprint Thirty-day monitoring of insulin-like growth factors and their binding proteins in intensive care unit patients
    R C Baxter
    Kolling Institute of Medical Research, Royal North Shore Hospital, St Leonards, NSW, Australia
    Growth Horm IGF Res 8:455-63. 1998
    ..By demonstrating that the IGF-I response in ICU patients is related to changes in the IGF regulatory proteins, this study may be of value in planning therapeutic intervention using growth hormone or IGF-I...
  28. ncbi request reprint Nuclear import of insulin-like growth factor-binding protein-3 and -5 is mediated by the importin beta subunit
    L J Schedlich
    Kolling Institute of Medical Research, University of Sydney, Royal North Shore Hospital, Sydney, New South Wales 2065, Australia
    J Biol Chem 275:23462-70. 2000
    ..Together these results suggest that the NLSs within the C-terminal domain of IGFBP-3 and IGFBP-5 are required for importin-beta-dependent nuclear uptake and probably also accumulation through mediating binding to nuclear components...
  29. ncbi request reprint Mutagenesis of basic amino acids in the carboxyl-terminal region of insulin-like growth factor binding protein-5 affects acid-labile subunit binding
    S M Firth
    Kolling Institute of Medical Research, University of Sydney, Royal North Shore Hospital, St Leonards, New South Wales 2065, Australia
    Endocrinology 142:2147. 2001
    ..Mutation of K(134)R(136) or K(138)K(139) in the central region of IGFBP-5 resulted in a small decrease in ALS binding...
  30. ncbi request reprint Oncogenic ras causes resistance to the growth inhibitor insulin-like growth factor binding protein-3 (IGFBP-3) in breast cancer cells
    J L Martin
    Kolling Institute of Medical Research, University of Sydney, Royal North Shore Hospital, St Leonards, New South Wales 2065, Australia
    J Biol Chem 274:16407-11. 1999
    ..These results suggest that MAP kinase activation by oncogenic ras expression causes IGFBP-3 resistance, a possible factor in the dysregulation of breast cancer cell growth...
  31. pmc Discovery of serum biomarkers for pancreatic adenocarcinoma using proteomic analysis
    A Xue
    Department of Surgery, University of Sydney, Royal North Shore Hospital, St Leonards, New South Wales 2065, Australia
    Br J Cancer 103:391-400. 2010
    ..The serum/plasma proteome was explored for biomarkers to improve the diagnostic ability of CA19-9 in pancreatic adenocarcinoma (PC)...
  32. pmc Serum apolipoprotein C-II is prognostic for survival after pancreatic resection for adenocarcinoma
    A Xue
    Department of Gastrointestinal Surgery, University of Sydney, Kolling Institute of Medical Research, St Leonards NSW 2065, Australia
    Br J Cancer 107:1883-91. 2012
    ..This study aimed to identify protein biomarkers prognostic for long-term survival and to validate them with clinico-pathological information...
  33. ncbi request reprint Purification and properties of a novel insulin-like growth factor-II binding protein from transformed human fibroblasts
    J L Martin
    Department of Endocrinology, Royal Prince Alfred Hospital, Camperdown, NSW, Australia
    J Biol Chem 265:4124-30. 1990
    ..We conclude that, although TFBP appears to share an epitope with IGFBP-3, it is distinctly different from any previously described IGF binding protein...
  34. ncbi request reprint Detection of growth hormone responsive proteins using SELDI-TOF mass spectrometry
    L Chung
    Kolling Institute of Medical Research, University of Sydney, Royal North Shore Hospital, St Leonards, NSW 2065, Australia
    Growth Horm IGF Res 19:383-7. 2009
    ..Results to date indicate that proteomic changes observed following GH administration have the potential to yield novel biomarker sets for the detection of GH abuse...
  35. ncbi request reprint The regulation of acid-labile subunit gene expression and secretion by cyclic adenosine 3',5'-monophosphate
    P J Delhanty
    Kolling Institute of Medical Research, University of Sydney, Royal North Shore Hospital, St Leonards, New South Wales, Australia
    Endocrinology 139:260-5. 1998
    ..Our study suggests that cAMP affects ALS gene transcription, perhaps by interrupting the GH signaling pathway, and also inhibits posttranscriptional events in ALS expression...
  36. ncbi request reprint Parenteral nutrition with lipid or glucose suppresses liver growth and response to GH in adolescent male rats
    A Sevette
    Department of Surgery, University of Sydney, Sydney, New South Wales 2065, Australia
    Am J Physiol Endocrinol Metab 281:E1063-72. 2001
    ..L-PN increased carcass lipid significantly and decreased hepatic steatosis. Nevertheless, PN caused significant liver steatosis and profound impairment of hepatic cell growth, which was associated with relative hepatic GH resistance...
  37. doi request reprint IGFBP-3 binds GRP78, stimulates autophagy and promotes the survival of breast cancer cells exposed to adverse microenvironments
    S Grkovic
    Kolling Institute of Medical Research, University of Sydney, Royal North Shore Hospital, St Leonards, New South Wales, Australia
    Oncogene 32:2412-20. 2013
    ..In conclusion, we have shown that IGFBP-3 stimulates autophagy and thereby promotes the survival of breast cancer cells exposed to conditions that represent the adverse microenvironments encountered by solid tumor cells in vivo...
  38. pmc Tissue biomarkers of breast cancer and their association with conventional pathologic features
    L Chung
    Hormones and Cancer Division, Kolling Institute of Medical Research, University of Sydney, Royal North Shore Hospital, St Leonards, New South Wales, Australia
    Br J Cancer 108:351-60. 2013
    ..This study aimed to identify tissue proteins that differentiate breast cancer tissue from healthy breast tissue using protein chip mass spectrometry and to examine associations with conventional pathological features...
  39. ncbi request reprint Changes in the IGF-IGFBP axis in critical illness
    R C Baxter
    Kolling Institute of Medical Research, University of Sydney, Royal North Shore Hospital, Sydney, NSW, 2065, Australia
    Best Pract Res Clin Endocrinol Metab 15:421-34. 2001
    ..Initial evidence suggests that the level of IGFBP-1 may be predictive of outcome in critically ill patients, suggesting a possible prognostic role for this protein...
  40. ncbi request reprint Insulin-like growth factor-binding protein-2 in patients with prostate carcinoma and benign prostatic hyperplasia
    P J Ho
    Kolling Institute of Medical Research, Royal North Shore Hospital, St Leonards, NSW, Australia
    Clin Endocrinol (Oxf) 46:333-42. 1997
    ....
  41. ncbi request reprint Molecular cloning of the acid-labile subunit of the rat insulin-like growth factor binding protein complex
    J Dai
    Department of Medicine, University of Sydney, NSW, Australia
    Biochem Biophys Res Commun 188:304-9. 1992
    ..4 kb and minor bands of about 2 kb, 1.4 kb and 1 kb. The tissue distribution of this protein may therefore be wider than previously recognized...
  42. pmc Hyperinsulinism and overgrowth without obesity
    S Srinivasan
    Sydney Children s Hospital, Sydney, Australia
    Arch Dis Child 88:332-4. 2003
    ..Over the course of two years observation she has developed acanthosis nigricans and diabetes mellitus...
  43. ncbi request reprint Nuclear insulin-like growth factor binding protein-3 induces apoptosis and is targeted to ubiquitin/proteasome-dependent proteolysis
    Frédéric R Santer
    Cell Metabolism and Differentiation Research Group, Institute for Biomedical Aging Research of the Austrian Academy of Sciences, Innsbruck, Austria
    Cancer Res 66:3024-33. 2006
    ..Moreover, we show that IGFBP-3, if ectopically expressed in the nucleus, can induce apoptotic cell death. These results suggest that ubiquitin/proteasome-mediated proteolysis of IGFBP-3 may contribute to down-regulation of apoptosis...
  44. ncbi request reprint Protein chip discovery of secreted proteins regulated by the phosphatidylinositol 3-kinase pathway in ovarian cancer cell lines
    Michelle Moscova
    Laboratory of Cellular and Diagnostic Proteomics, Kolling Institute of Medical Research, University of Sydney, Royal North Shore Hospital, St Leonards, New South Wales 2065, Australia
    Cancer Res 66:1376-83. 2006
    ..We conclude that proteomic analysis of cell models of human disease may facilitate the discovery of pathway-dependent proteins...
  45. ncbi request reprint Insulin-like growth factor binding protein-3 leads to insulin resistance in adipocytes
    Sophie S Y Chan
    Kolling Institute of Medical Research, Royal North Shore Hospital, Pacific Highway, St Leonards 2065, NSW, Australia
    J Clin Endocrinol Metab 90:6588-95. 2005
    ..Transgenic mice overexpressing IGF binding protein-3 (IGFBP-3) have insulin resistance with reduced uptake of 2-deoxyglucose in muscle and adipose tissue...
  46. ncbi request reprint The glycemic index of foods influences postprandial insulin-like growth factor-binding protein responses in lean young subjects
    Jennie C Brand-Miller
    Human Nutrition Unit, School of Molecular and Microbial Biosciences, University of Sydney, Sydney, Australia
    Am J Clin Nutr 82:350-4. 2005
    ..We hypothesized that IGF and IGF-binding protein (IGFBP) responses may be acutely affected by differences in the glycemic index (GI) of foods...
  47. ncbi request reprint Enhancement of tumor necrosis factor-alpha-induced growth inhibition by insulin-like growth factor-binding protein-5 (IGFBP-5), but not IGFBP-3 in human breast cancer cells
    Alison J Butt
    Kolling Institute of Medical Research, University of Sydney, Royan North Shore Hospital, New South Wales 2065, Australia
    Endocrinology 146:3113-22. 2005
    ..Furthermore, although IGFBP-3 and IGFBP-5 share much structural and functional homology, they can modulate distinct apoptotic pathways in human breast cancer cells...
  48. ncbi request reprint The aminoterminal insulin-like growth factor (IGF) binding domain of IGF binding protein-3 cannot be functionally substituted by the structurally homologous domain of CCN3
    Xiaolang Yan
    Kolling Institute of Medical Research, Royal North Shore Hospital, St Leonards, New South Wales 2065, Australia
    Endocrinology 147:5268-74. 2006
    ....
  49. ncbi request reprint Expression of insulin-like growth factor binding protein-2 by MCF-7 breast cancer cells is regulated through the phosphatidylinositol 3-kinase/AKT/mammalian target of rapamycin pathway
    Janet L Martin
    Kolling Institute of Medical Research, University of Sydney, Royal North Shore Hospital, St Leonards, New South Wales 2065, Australia
    Endocrinology 148:2532-41. 2007
    ..These findings indicate that IGFBP-2 is regulated predominantly through the phosphatidylinositol 3-kinase/Akt/mammalian target of rapamycin pathway, the target of a number of anticancer agents currently in clinical trial and use...
  50. ncbi request reprint The in vivo phosphorylation and glycosylation of human insulin-like growth factor-binding protein-5
    Mark E Graham
    Cell Signalling Unit, Children s Medical Research Institute, Locked Bag 23, Wentworthville, New South Wales 2145, Australia
    Mol Cell Proteomics 6:1392-405. 2007
    ..The results reveal the first description of the in vivo phosphorylation of IGFBP-5 and its glycan composition...
  51. ncbi request reprint Insulin-like growth factor binding protein-5 interacts with the vitamin D receptor and modulates the vitamin D response in osteoblasts
    Lynette J Schedlich
    Kolling Institute of Medical Research, Royal North Shore Hospital, St Leonards, New South Wales 2065, Australia
    Mol Endocrinol 21:2378-90. 2007
    ....
  52. ncbi request reprint Molecular basis of the interaction between IGFBP-3 and retinoid X receptor: role in modulation of RAR-signaling
    Lynette J Schedlich
    Kolling Institute of Medical Research, University of Sydney, Royal North Shore Hospital, St Leonards, NSW 2065, Australia
    Arch Biochem Biophys 465:359-69. 2007
    ..We conclude that residues in both the N- and C-terminal domains of IGFBP-3 are involved in binding the retinoid receptors, and that this interaction is essential to the modulation of RAR-signaling by IGFBP-3...
  53. ncbi request reprint Profiling of apoptotic changes in human breast cancer cells using SELDI-TOF mass spectrometry
    Sharon Leong
    Kolling Institute of Medical Research, The University of Sydney, Royal North Shore Hospital, Sydney, Australia
    Cell Physiol Biochem 20:579-90. 2007
    ..Further validation using patient samples is required to confirm their potential utility in monitoring the effectiveness of anti-cancer drugs in inducing tumour cell apoptosis...
  54. doi request reprint Pharmacodynamics of growth hormone abuse biomarkers and the influence of gender and testosterone: a randomized double-blind placebo-controlled study in young recreational athletes
    Anne E Nelson
    Pituitary Research Unit, Garvan Institute of Medical Research, St Vincent s Hospital, 384 Victoria Street, Darlinghurst 2010, Sydney, NSW, Australia
    J Clin Endocrinol Metab 93:2213-22. 2008
    ..IGF axis proteins and collagen peptides are promising markers of GH abuse...
  55. doi request reprint Rapamycin treatment for a child with germline PTEN mutation
    Deborah J Marsh
    Kolling Institute of Medical Research, University of Sydney and Royal North Shore Hospital, Sydney, NSW, Australia
    Nat Clin Pract Oncol 5:357-61. 2008
    ..Over the first years of life, the patient developed life-threatening respiratory dysfunction and malnutrition because of progressive growth of hamartomas affecting the chest, mediastinum, abdomen and pelvis...
  56. doi request reprint Within-subject variability and analytic imprecision of insulinlike growth factor axis and collagen markers: implications for clinical diagnosis and doping tests
    Tuan V Nguyen
    Garvan Institute of Medical Research and Department of Endocrinology, Sydney, Australia
    Clin Chem 54:1268-76. 2008
    ..We sought to determine short-term within-subject variability of these markers in a large cohort of healthy individuals...
  57. ncbi request reprint Altered expression of members of the IGF-axis in clear cell renal cell carcinoma
    Masayuki Takahashi
    Laboratory of Cancer Genetics, Van Andel Research Institute, 333 Bostwick NE, Grand Rapids, MI 49503, USA
    Int J Oncol 26:923-31. 2005
    ..In addition, we demonstrate that the expression of the A isoform of the insulin receptor is significantly upregulated, while that of IGFBP-5 are significantly downregulated in this tumour subtype...
  58. ncbi request reprint Role of N- and C-terminal residues of insulin-like growth factor (IGF)-binding protein-3 in regulating IGF complex formation and receptor activation
    Xiaolang Yan
    Kolling Institute of Medical Research, University of Sydney, Royal North Shore Hospital, St Leonards, New South Wales 2065, Australia
    J Biol Chem 279:53232-40. 2004
    ..This suggests that residual binding activity of IGFBP-3 mutants may still be sufficient to inhibit IGF biological activity and questions the use of such analogs to study IGF-independent effects of IGFBP-3...
  59. ncbi request reprint Signaling through the Smad pathway by insulin-like growth factor-binding protein-3 in breast cancer cells. Relationship to transforming growth factor-beta 1 signaling
    Susan Fanayan
    Kolling Institute of Medical Research, University of Sydney, Royal North Shore Hospital, St Leonards 2065, New South Wales, Australia
    J Biol Chem 277:7255-61. 2002
    ..The precise mechanism by which IGFBP-3 interacts with the TGF-beta receptor system remains to be established...
  60. ncbi request reprint The circulating IGF system and its relationship with 24-h glucose regulation and insulin sensitivity in healthy subjects
    Jan Frystyk
    Medical Research Laboratories and Medical Department M, Aarhus University Hospital, Aarhus C, Denmark
    Clin Endocrinol (Oxf) 58:777-84. 2003
    ..Insulin sensitivity was estimated at the end of each 24-h study period by use of the hyperinsulinaemic euglycaemic clamp technique...
  61. ncbi request reprint Insulin-like growth factor-binding protein-5 inhibits the growth of human breast cancer cells in vitro and in vivo
    Alison J Butt
    Kolling Institute of Medical Research, University of Sydney, Royal North Shore Hospital, St Leonards, New South Wales 2065, Australia
    J Biol Chem 278:29676-85. 2003
    ..These data suggest that IGFBP-5 is a potent growth inhibitor and proapoptotic agent in human breast cancer cells via modulation of cell cycle regulation and apoptotic mediators...
  62. ncbi request reprint Regulation of the acid-labile subunit in sustained endotoxemia
    Sung Eun Kong
    Kolling Institute of Medical Research, University of Sydney, Royal North Shore Hospital, St Leonards, New South Wales 2065, Australia
    Am J Physiol Endocrinol Metab 283:E692-701. 2002
    ..These data may indicate that, in sustained endotoxemia, ALS levels are regulated through modulation of hepatic insulin sensitivity...
  63. ncbi request reprint Phosphorylation of insulin-like growth factor binding protein-3 by deoxyribonucleic acid-dependent protein kinase reduces ligand binding and enhances nuclear accumulation
    Lynette J Schedlich
    Kolling Institute of Medical Research, University of Sydney, Royal North Shore Hospital, Sydney, New South Wales 2065, Australia
    Endocrinology 144:1984-93. 2003
    ....
  64. ncbi request reprint Insulin-like growth factor-binding protein-3 potentiates epidermal growth factor action in MCF-10A mammary epithelial cells. Involvement of p44/42 and p38 mitogen-activated protein kinases
    Janet L Martin
    Kolling Institute of Medical Research, University of Sydney, Royal North Shore Hospital, St Leonards, New South Wales 2065, Australia
    J Biol Chem 278:2969-76. 2003
    ..Collectively, these findings indicate that IGFBP-3 enhances EGF signaling and proliferative effects in breast epithelial cells via increased EGF receptor phosphorylation and activation of p44/42 and p38 MAP kinase signaling pathways...
  65. ncbi request reprint Metformin rapidly increases insulin receptor activation in human liver and signals preferentially through insulin-receptor substrate-2
    Jenny E Gunton
    Kolling Institute of Medical Research, University of Sydney, Royal North Shore Hospital, St Leonards, Sydney, New South Wales 2065 Australia
    J Clin Endocrinol Metab 88:1323-32. 2003
    ..The effect of metformin was completely blocked by an IR inhibitor...
  66. ncbi request reprint Age-dependent regulation of the acid-labile subunit in response to fasting-refeeding in rats
    Sung Eun Kong
    Kolling Institute of Medical Research, University of Sydney, Royal North Shore Hospital, St Leonards, New South Wales 2065, Australia
    Endocrinology 143:4505-12. 2002
    ..We hypothesize that development-dependent regulation of ALS and IGF-I during refeeding may involve a posttranscriptional hepatic response that is not GH dependent...
  67. ncbi request reprint Regulation of insulin-like growth factor binding protein-1 during protracted critical illness
    Dieter Mesotten
    Department of Intensive Care Medicine, University Hospital Gasthuisberg, Catholic University Leuven, B 3000 Leuven, Belgium
    J Clin Endocrinol Metab 87:5516-23. 2002
    ..These data suggest that hepatic insulin resistance in prolonged critically ill patients, reflected by high serum IGFBP-1 levels, is not overcome by intensive insulin therapy, and that this may affect patient outcome...
  68. ncbi request reprint Insulin-like growth factor-I inhibits cell growth in the a549 non-small lung cancer cell line
    Yuzo Kodama
    Kolling Institute of Medical Research, University of Sydney, Royal North Shore Hospital, St Leonards, New South Wales, Australia
    Am J Respir Cell Mol Biol 27:336-44. 2002
    ..Collectively, these data suggest that IGF-I is growth inhibitory to A549 cells, possibly via sustained activation of the PI 3'-kinase signaling pathway, and induction of p21(Cip1/WAF1)...
  69. ncbi request reprint IGF-binding protein-3-induced growth inhibition and apoptosis do not require cell surface binding and nuclear translocation in human breast cancer cells
    Alison J Butt
    Kolling Institute of Medical Research, University of Sydney, Royal North Shore Hospital, St Leonards, New South Wales 2065, Australia
    Endocrinology 143:2693-9. 2002
    ..This suggests the existence of multiple pathways by which IGFBP-3 elicits its growth effects...
  70. ncbi request reprint Amino- and carboxyl-terminal fragments of insulin-like growth factor (IGF) binding protein-3 cooperate to bind IGFs with high affinity and inhibit IGF receptor interactions
    Louis D Payet
    Kolling Institute of Medical Research, University of Sydney, Royal North Shore Hospital, St Leonards, New South Wales 2065, Australia
    Endocrinology 144:2797-806. 2003
    ..This study demonstrates that isolated amino-terminal and carboxyl-terminal domains of IGFBP-3 cooperate in the presence of IGFs to form high-affinity complexes that retain the ability to block IGF activity...
  71. ncbi request reprint Impaired blockade of insulin-like growth factor I (IGF-I)-induced hypoglycemia by IGF binding protein-3 analog with reduced ternary complex-forming ability
    Sue M Firth
    Kolling Institute of Medical Research, University of Sydney, Royal North Shore Hospital, St Leonards, New South Wales 2065, Australia
    Endocrinology 143:1669-76. 2002
    ..These data confirm the hypothesis that regulation of IGF-I bioactivity in vivo by IGFBP-3 depends on its ability to form ternary complexes...
  72. ncbi request reprint Regulation of the somatotropic axis by intensive insulin therapy during protracted critical illness
    Dieter Mesotten
    Department of Intensive Care Medicine, University Hospital Gasthuisberg, B 3000 Leuven, Belgium
    J Clin Endocrinol Metab 89:3105-13. 2004
    ..GH resistance accompanied this suppression of the IGF-I axis. To what extent and through which mechanisms the changes in the GH-IGF-IGFBP axis contributed to the survival benefit under intensive insulin therapy remain elusive...
  73. ncbi request reprint Growth hormone rapidly induces resistin gene expression in white adipose tissue of spontaneous dwarf (SDR) rats
    Patric J D Delhanty
    Kolling Institute of Medical Research, Royal North Shore Hospital, University of Sydney, St Leonards, NSW, Australia
    Endocrinology 143:2445-8. 2002
    ..Our data demonstrate an unexpected marked, rapid and sustained up-regulation of resistin by GH. This may indicate a role for resistin in GH-dependent metabolic and differentiative effects in WAT...
  74. ncbi request reprint The role of the acid-labile subunit in regulating insulin-like growth factor transport across human umbilical vein endothelial cell monolayers
    Louis D Payet
    Kolling Institute of Medical Research, University of Sydney, Royal North Shore Hospital, St Leonards, New South Wales 2065, Australia
    J Clin Endocrinol Metab 89:2382-9. 2004
    ..Our data suggest that pregnancy-proteolyzed IGFBP-3, despite forming normal ternary complexes, is less effective than intact IGFBP-3 in retarding IGF egress from the circulation...
  75. ncbi request reprint Insulin-like growth factor binding protein-3 expression is associated with growth stimulation of T47D human breast cancer cells: the role of altered epidermal growth factor signaling
    Alison J Butt
    Kolling Institute of Medical Research, University of Sydney, Royal North Shore Hospital, St Leonards, New South Wales 2065, Australia
    J Clin Endocrinol Metab 89:1950-6. 2004
    ....
  76. ncbi request reprint Insulin-like growth factor binding protein-3 prevents retinoid receptor heterodimerization: implications for retinoic acid-sensitivity in human breast cancer cells
    Lynette J Schedlich
    Kolling Institute of Medical Research, University of Sydney, Royal North Shore Hospital, NSW, Australia
    Biochem Biophys Res Commun 314:83-8. 2004
    ..Thus, IGFBP-3 has the potential to reduce the RARE-mediated transactivation of target genes and modulate the atRA-response in BCCs...
  77. ncbi request reprint Regulation of the growth hormone receptor/binding protein, insulin-like growth factor ternary complex system in human cirrhosis
    Anthony J Donaghy
    Kolling Institute of Medical Research, University of Sydney, Royal North Shore Hospital, St Leonards, Sydney, NSW 2065, Australia
    J Hepatol 36:751-8. 2002
    ..We examined the impact of cirrhosis on hepatic mRNA and serum protein levels for the GH receptor (GHR)/binding protein (GHBP), IGF-I, IGF binding protein (IGFBP)-3 and the acid-labile subunit (ALS)...
  78. ncbi request reprint The combined administration of GH-releasing peptide-2 (GHRP-2), TRH and GnRH to men with prolonged critical illness evokes superior endocrine and metabolic effects compared to treatment with GHRP-2 alone
    Greet Van den Berghe
    Department of Intensive Care Medicine, University Hospital Gasthuisberg, University of Leuven, B 3000 Leuven, Belgium
    Clin Endocrinol (Oxf) 56:655-69. 2002
    ..This study evaluated the impact of combined treatment with GH-releasing peptide-2 (GHRP-2), TRH and GnRH for 5 days compared with GHRP-2 + TRH and with GHRP-2 alone...
  79. ncbi request reprint Over expression of insulin-like growth factor binding protein 3 in clear cell renal cell carcinoma
    Shang Tian Chuang
    Department of Pathology, Northwestern Memorial Hospital, Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA
    J Urol 179:445-9. 2008
    ..We further investigated IGFBP-3 expression in renal tumors using gene expression microarrays, immunohistochemistry and Western blotting...
  80. ncbi request reprint Cellular actions of the insulin-like growth factor binding proteins
    Sue M Firth
    Kolling Institute of Medical Research, University of Sydney, Royal North Shore Hospital, St Leonards, New South Wales 2065, Australia
    Endocr Rev 23:824-54. 2002
    ..Because IGFBPs regulate cell functions by diverse mechanisms, manipulation of IGFBP-regulated pathways is speculated to offer therapeutic opportunities in cancer and other diseases...
  81. ncbi request reprint A study of parenteral versus enteral nutrition following caecal ligation and puncture in the rat: Influence on survival and tissue protein turnover
    Christopher J Scarlett
    Department of Surgery, Royal North Shore Hospital, University of Sydney, St Leonards NSW 2065, Australia
    Clin Nutr 23:1135-45. 2004
    ..Methods of nutritional management in abdominal sepsis remain controversial...
  82. ncbi request reprint Does growth hormone allow more efficient nitrogen sparing in postoperative patients requiring parenteral nutrition? A double-blind, placebo-controlled randomised trial
    Andre Sevette
    University of Sydney, Departments of Surgery, North Shore Hospital, Sydney, St Leonards, NSW 2065, Australia
    Clin Nutr 24:943-55. 2005
    ..Unfortunately, the negative clinical outcome of GH treatment in intensive care patients limits its use in this setting, but demands answers to the mechanism behind the action of this therapy...
  83. ncbi request reprint Novel biomarkers of human growth hormone action from serum proteomic profiling using protein chip mass spectrometry
    Liping Chung
    Kolling Institute of Medical Research, Royal North Shore Hospital, St Leonards, New South Wales 2065, Australia
    J Clin Endocrinol Metab 91:671-7. 2006
    ..Measurement of novel GH-regulated serum protein biomarkers might provide an indirect method to detect exogenous GH...
  84. ncbi request reprint Growth hormone modulation of the rat hepatic bile transporter system in endotoxin-induced cholestasis
    Dieter Mesotten
    Kolling Institute of Medical Research, University of Sydney, Royal North Shore Hospital, St Leonards, New South Wales 2065, Australia
    Endocrinology 144:4008-17. 2003
    ..This study of endotoxinemia may thus provide a mechanistic link between GH treatment and exacerbation of cholestasis through modulation of basolateral bile acid transporter expression in the rat hepatocyte...
  85. ncbi request reprint 1,4-Benzodiazepines as inhibitors of respiratory syncytial virus
    Malcolm C Carter
    Arrow Therapeutics Ltd, Britannia House, 7, Trinity Street, London, SE1 1DA, UK
    J Med Chem 49:2311-9. 2006
    ..The most potent compounds identified from this work were A-58568 (47), A-58569 (44), and A-62066 (46), where modifications to the aromatic substitution enhanced potency, and A-58175 (42), where the amide linker was modified...
  86. ncbi request reprint Proteomic classification of pancreatic adenocarcinoma tissue using protein chip technology
    Christopher J Scarlett
    Department of Surgery, University of Sydney, Royal North Shore Hospital, St Leonards, New South Wales, Australia
    Gastroenterology 130:1670-8. 2006
    ....
  87. ncbi request reprint 1,4-benzodiazepines as inhibitors of respiratory syncytial virus. The identification of a clinical candidate
    Elisa A Henderson
    Arrow Therapeutics, Britannia House, 7 Trinity Street, London, SE1 1DA, United Kingdom
    J Med Chem 50:1685-92. 2007
    ..Compound 17b also possessed a good pharmacokinetic profile and has now progressed into the clinic...
  88. pmc RSV604, a novel inhibitor of respiratory syncytial virus replication
    Joanna Chapman
    Arrow Therapeutics Ltd, Britannia House, 7 Trinity Street, London SE1 1DB, United Kingdom
    Antimicrob Agents Chemother 51:3346-53. 2007
    ..RSV604, which is currently in phase II clinical trials, represents the first in a new class of RSV inhibitors and may have significant potential for the effective treatment of RSV disease...
  89. ncbi request reprint Classification of pancreatic cystic lesions using SELDI-TOF mass spectrometry
    Christopher J Scarlett
    Department of Surgery, University of Sydney, Sydney, New South Wales, Australia
    ANZ J Surg 77:648-53. 2007
    ..This preliminary study aimed to analyse pancreatic cyst fluid, using a proteomic approach, to generate reproducible protein profiles to assist in the classification of malignant and non-carcinoma samples...
  90. ncbi request reprint Toward the development of a test for growth hormone (GH) abuse: a study of extreme physiological ranges of GH-dependent markers in 813 elite athletes in the postcompetition setting
    Marie Louise Healy
    Department of Diabetes and Endocrinology, GKT School of Medicine, St Thomas Hospital, London, UK
    J Clin Endocrinol Metab 90:641-9. 2005
    ..Normative data applicable to white athletes are provided. This provides important groundwork for the development of a test for GH abuse, although these values may be specific for the reagents and assays used...