Mark A Baker

Summary

Affiliation: University of Newcastle
Country: Australia

Publications

  1. Baker M, Smith N, Hetherington L, Taubman K, Graham M, Robinson P, et al. Label-free quantitation of phosphopeptide changes during rat sperm capacitation. J Proteome Res. 2010;9:718-29 pubmed publisher
    ..This change, together with the other protein identifications reported in this study, constitute important new leads in elucidating the biochemical mechanisms by which spermatozoa attain a capacitated state. ..
  2. Baker M, Nixon B, Naumovski N, Aitken R. Proteomic insights into the maturation and capacitation of mammalian spermatozoa. Syst Biol Reprod Med. 2012;58:211-7 pubmed publisher
    ..As a result of such advances, insights will be generated that should ultimately lead to significant improvements in our capacity to diagnose and treat the infertile male. ..
  3. Baker M, Weinberg A, Hetherington L, Villaverde A, Velkov T. Analysis of protein thiol changes occurring during rat sperm epididymal maturation. Biol Reprod. 2015;92:11 pubmed publisher
    ..Interestingly, two glycolytic enzymes, hexokinase-1 and lactate dehydrogenase, also display increased cysteine oxidation during epididymal transit, which may be involved in the regulation of the enzyme activities. ..
  4. Baker M, Weinberg A, Hetherington L, Villaverde A, Velkov T, Baell J, et al. Defining the mechanisms by which the reactive oxygen species by-product, 4-hydroxynonenal, affects human sperm cell function. Biol Reprod. 2015;92:108 pubmed publisher
    ..Given that epigenetic regulation is likely affected in oxidative-stressed spermatozoa, this data show that spermatozoa appear to shut down under these conditions before reaching the egg. ..
  5. Carbone V, Schneider E, Rockman S, Baker M, Huang J, Ong C, et al. Molecular Characterisation of the Haemagglutinin Glycan-Binding Specificity of Egg-Adapted Vaccine Strains of the Pandemic 2009 H1N1 Swine Influenza A Virus. Molecules. 2015;20:10415-34 pubmed publisher
    ..Collectively, the glycan binding data inform future vaccine design strategies to introduce the D225G or Q226R amino acid substitutions into recombinant H1N1 viruses. ..
  6. Baker M. Proteomics of post-translational modifications of mammalian spermatozoa. Cell Tissue Res. 2016;363:279-87 pubmed publisher
    ..In addition, several other PTM such as disulfide cross-linking, ubiquitination, acetylation and methylation all play a role to both develop and enable a spermatozoon to achieve its final destiny. ..
  7. Baker M, Hetherington L, Weinberg A, Velkov T. Phosphopeptide analysis of rodent epididymal spermatozoa. J Vis Exp. 2014;: pubmed publisher
    ..Once isolated, phosphopeptides can be injected into MS to identify both protein phosphorylation events on specific amino acid residues and quantify the levels of phosphorylation taking place during the sperm maturation processes. ..
  8. Tanphaichitr N, Srakaew N, Alonzi R, Kiattiburut W, Kongmanas K, Zhi R, et al. Potential Use of Antimicrobial Peptides as Vaginal Spermicides/Microbicides. Pharmaceuticals (Basel). 2016;9: pubmed publisher
    ..Furthermore, the spermicidal effects of LL-37 have been demonstrated in vivo in mice. Therefore, the availability of LL-37 as a vaginal spermicide/microbicide will empower women for self-protection against unwanted pregnancies and STI. ..
  9. Villaverde A, Hetherington L, Baker M. Quantitative Glycopeptide Changes in Rat Sperm During Epididymal Transit. Biol Reprod. 2016;94:91 pubmed publisher
    ..The results from this study showed that changes in the N-linked sialoglycoprotein profile is a major hallmark of sperm maturation in rats. ..

More Information

Publications14

  1. Baker M, Hetherington L, Weinberg A, Naumovski N, Velkov T, Pelzing M, et al. Analysis of phosphopeptide changes as spermatozoa acquire functional competence in the epididymis demonstrates changes in the post-translational modification of Izumo1. J Proteome Res. 2012;11:5252-64 pubmed publisher
    ..A model is advanced suggesting that these phospho-regulations are likely to act as a scaffold for the association of adaptor proteins with Izumo1 as these cells prepare for fertilization. ..
  2. Baker M, Naumovski N, Hetherington L, Weinberg A, Velkov T, Aitken R. Head and flagella subcompartmental proteomic analysis of human spermatozoa. Proteomics. 2013;13:61-74 pubmed publisher
  3. Young S, Miyata H, Satouh Y, Kato H, Nozawa K, Isotani A, et al. CRISPR/Cas9-Mediated Rapid Generation of Multiple Mouse Lines Identified Ccdc63 as Essential for Spermiogenesis. Int J Mol Sci. 2015;16:24732-50 pubmed publisher
    ..This study demonstrates the versatility of the CRISPR/Cas9 system to generate animal models of a highly complex system by introducing point mutations and simple knockouts in a fast and efficient manner. ..
  4. Baker M, Smith N, Hetherington L, Pelzing M, Condina M, Aitken R. Use of titanium dioxide to find phosphopeptide and total protein changes during epididymal sperm maturation. J Proteome Res. 2011;10:1004-17 pubmed publisher
    ..These data provide mechanistic insight into the means by which spermatozoa acquire functionality during epididymal transit. ..
  5. Hetherington L, Schneider E, Scott C, DeKretser D, Muller C, Hondermarck H, et al. Deficiency in Outer Dense Fiber 1 Is a Marker and Potential Driver of Idiopathic Male Infertility. Mol Cell Proteomics. 2016;15:3685-3693 pubmed
    ..Hence, a strong reduction ODF1 is a marker of idiopathic male infertility and a potential driver of this condition. ..