Mark A Baker

Summary

Affiliation: University of Newcastle
Country: Australia

Publications

  1. doi request reprint Analysis of phosphopeptide changes as spermatozoa acquire functional competence in the epididymis demonstrates changes in the post-translational modification of Izumo1
    Mark A Baker
    Priority Research Centre in Reproductive Science, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, 2308, Australia
    J Proteome Res 11:5252-64. 2012
  2. doi request reprint Head and flagella subcompartmental proteomic analysis of human spermatozoa
    Mark A Baker
    Priority Research Centre in Reproductive Science, School of Environmental and Life Sciences, The University of Newcastle, Callaghan, NSW, Australia
    Proteomics 13:61-74. 2013
  3. doi request reprint Proteomic insights into the maturation and capacitation of mammalian spermatozoa
    Mark A Baker
    PRC in Reproductive Science, School of Environmental and Life Sciences, University of Newcastle, NSW, Australia
    Syst Biol Reprod Med 58:211-7. 2012
  4. doi request reprint Use of titanium dioxide to find phosphopeptide and total protein changes during epididymal sperm maturation
    Mark A Baker
    The ARC Centre of Excellence in Biotechnology and Development, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, 2308, Australia
    J Proteome Res 10:1004-17. 2011
  5. doi request reprint The mouse sperm proteome characterized via IPG strip prefractionation and LC-MS/MS identification
    Mark A Baker
    The ARC Centre of Excellence in Biotechnology and Development, Reproductive Science Group, School of Environmental and Life Sciences, University of Newcastle, NSW, Australia
    Proteomics 8:1720-30. 2008
  6. pmc Reactive oxygen species in spermatozoa: methods for monitoring and significance for the origins of genetic disease and infertility
    Mark A Baker
    The ARC Centre of Excellence in Biotechnology and Development, Reproductive Science Group, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW 2308, Australia
    Reprod Biol Endocrinol 3:67. 2005
  7. doi request reprint The rat sperm proteome characterized via IPG strip prefractionation and LC-MS/MS identification
    Mark A Baker
    The ARC Centre of Excellence in Biotechnology and Development, Reproductive Science Group, School of Environmental and Life Sciences, University of Newcastle, NSW, Australia
    Proteomics 8:2312-21. 2008
  8. pmc The 'omics revolution and our understanding of sperm cell biology
    Mark A Baker
    School of Environmental and Life Sciences, University of Newcastle Research Fellow, Priority Research Centre in Reproductive Science, University of Newcastle, Callaghan, NSW 2308, Australia
    Asian J Androl 13:6-10. 2011
  9. doi request reprint Label-free quantitation of phosphopeptide changes during rat sperm capacitation
    Mark A Baker
    The ARC Centre of Excellence in Biotechnology and Development, Priority Research Centre in Reproductive Science, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, 2308, Australia
    J Proteome Res 9:718-29. 2010
  10. doi request reprint Proteomic insights into spermatozoa: critiques, comments and concerns
    Mark A Baker
    ARC Centre of Excellence in Biotechnology and Development, Discipline of Biological Sciences, School of Environmental and Life Sciences, University of Newcastle, Callaghan, New South Wales 2308, Australia
    Expert Rev Proteomics 6:691-705. 2009

Detail Information

Publications41

  1. doi request reprint Analysis of phosphopeptide changes as spermatozoa acquire functional competence in the epididymis demonstrates changes in the post-translational modification of Izumo1
    Mark A Baker
    Priority Research Centre in Reproductive Science, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, 2308, Australia
    J Proteome Res 11:5252-64. 2012
    ..A model is advanced suggesting that these phospho-regulations are likely to act as a scaffold for the association of adaptor proteins with Izumo1 as these cells prepare for fertilization...
  2. doi request reprint Head and flagella subcompartmental proteomic analysis of human spermatozoa
    Mark A Baker
    Priority Research Centre in Reproductive Science, School of Environmental and Life Sciences, The University of Newcastle, Callaghan, NSW, Australia
    Proteomics 13:61-74. 2013
    ....
  3. doi request reprint Proteomic insights into the maturation and capacitation of mammalian spermatozoa
    Mark A Baker
    PRC in Reproductive Science, School of Environmental and Life Sciences, University of Newcastle, NSW, Australia
    Syst Biol Reprod Med 58:211-7. 2012
    ..As a result of such advances, insights will be generated that should ultimately lead to significant improvements in our capacity to diagnose and treat the infertile male...
  4. doi request reprint Use of titanium dioxide to find phosphopeptide and total protein changes during epididymal sperm maturation
    Mark A Baker
    The ARC Centre of Excellence in Biotechnology and Development, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, 2308, Australia
    J Proteome Res 10:1004-17. 2011
    ..These data provide mechanistic insight into the means by which spermatozoa acquire functionality during epididymal transit...
  5. doi request reprint The mouse sperm proteome characterized via IPG strip prefractionation and LC-MS/MS identification
    Mark A Baker
    The ARC Centre of Excellence in Biotechnology and Development, Reproductive Science Group, School of Environmental and Life Sciences, University of Newcastle, NSW, Australia
    Proteomics 8:1720-30. 2008
    ....
  6. pmc Reactive oxygen species in spermatozoa: methods for monitoring and significance for the origins of genetic disease and infertility
    Mark A Baker
    The ARC Centre of Excellence in Biotechnology and Development, Reproductive Science Group, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW 2308, Australia
    Reprod Biol Endocrinol 3:67. 2005
    ..In this review, we consider the possible origins of oxidative damage to human spermatozoa and reflect on the important contribution such stress might make to the origins of genetic disease in our species...
  7. doi request reprint The rat sperm proteome characterized via IPG strip prefractionation and LC-MS/MS identification
    Mark A Baker
    The ARC Centre of Excellence in Biotechnology and Development, Reproductive Science Group, School of Environmental and Life Sciences, University of Newcastle, NSW, Australia
    Proteomics 8:2312-21. 2008
    ..This inventory of gene products, the first ever 2-D LC-MS/MS analysis of rat spermatozoa, will be invaluable in directing future research into the molecular mechanisms that drive these highly specialized cells...
  8. pmc The 'omics revolution and our understanding of sperm cell biology
    Mark A Baker
    School of Environmental and Life Sciences, University of Newcastle Research Fellow, Priority Research Centre in Reproductive Science, University of Newcastle, Callaghan, NSW 2308, Australia
    Asian J Androl 13:6-10. 2011
    ....
  9. doi request reprint Label-free quantitation of phosphopeptide changes during rat sperm capacitation
    Mark A Baker
    The ARC Centre of Excellence in Biotechnology and Development, Priority Research Centre in Reproductive Science, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, 2308, Australia
    J Proteome Res 9:718-29. 2010
    ..This change, together with the other protein identifications reported in this study, constitute important new leads in elucidating the biochemical mechanisms by which spermatozoa attain a capacitated state...
  10. doi request reprint Proteomic insights into spermatozoa: critiques, comments and concerns
    Mark A Baker
    ARC Centre of Excellence in Biotechnology and Development, Discipline of Biological Sciences, School of Environmental and Life Sciences, University of Newcastle, Callaghan, New South Wales 2308, Australia
    Expert Rev Proteomics 6:691-705. 2009
    ..Three comprehensive liquid chromatography-mass spectrometry lists of human, mouse and rat spermatozoa are then compared, insights into the molecular regulation of sperm function discussed and future directions speculated upon...
  11. ncbi request reprint Redox activity associated with the maturation and capacitation of mammalian spermatozoa
    R John Aitken
    ARC Centre of Excellence in Biotechnology and Development, Discipline of Biological Sciences, University of Newcastle, New South Wales, Australia
    Free Radic Biol Med 36:994-1010. 2004
    ....
  12. ncbi request reprint Identification of cytochrome-b5 reductase as the enzyme responsible for NADH-dependent lucigenin chemiluminescence in human spermatozoa
    Mark A Baker
    The ARC Centre of Excellence in Biotechnology and Development, Reproductive Science Group, School of Environmental and Life Sciences, and Hunter Medical Research Institute, University of Newcastle, Callaghan, New South Wales 2308, Australia
    Biol Reprod 73:334-42. 2005
    ..These results emphasize the importance of the direct NADH-dependent reduction of these putative superoxide-sensitive probes by cytochrome-b5 reductase even though this enzyme does not, on its own accord, produce reactive oxygen species...
  13. ncbi request reprint Identification of post-translational modifications that occur during sperm maturation using difference in two-dimensional gel electrophoresis
    Mark A Baker
    The ARC Centre of Excellence in Biotechnology and Development, Reproductive Science Group, School of Environmental and Life Sciences, University of Newcastle, NSW, Australia
    Proteomics 5:1003-12. 2005
    ..We suggest that phosphorylation of this particular protein in a cAMP-dependent manner may contribute to the mechanisms by which motility is conferred upon spermatozoa...
  14. doi request reprint Investigation of the role of SRC in capacitation-associated tyrosine phosphorylation of human spermatozoa
    Lisa A Mitchell
    Reproductive Science Group, Discipline of Biological Sciences, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW 2308, Australia
    Mol Hum Reprod 14:235-43. 2008
    ..However, although the inhibition of PKA was also accompanied by a suppression of sperm motility, SRC inhibition did not induce a similar response...
  15. doi request reprint Phosphorylation and consequent stimulation of the tyrosine kinase c-Abl by PKA in mouse spermatozoa; its implications during capacitation
    Mark A Baker
    ARC Centre of Excellence in Biotechnology and Development, Reproductive Science Group, School of Environmental and Life Sciences, University of Newcastle, NSW 2308, Australia
    Dev Biol 333:57-66. 2009
    ..We present a model, whereby these kinases may operate together with SRC to ensure optimal levels of tyrosine phosphorylation in the sperm flagellum during the attainment of a capacitated state...
  16. ncbi request reprint Identification of SRC as a key PKA-stimulated tyrosine kinase involved in the capacitation-associated hyperactivation of murine spermatozoa
    Mark A Baker
    The ARC Centre of Excellence in Biotechnology and Development, Reproductive Science Group, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW 2308, Australia
    J Cell Sci 119:3182-92. 2006
    ..The latter was localized to the acrosome and flagellum of murine spermatozoa by immunocytochemistry, whereas capacitation was associated with an inactivating serine phosphosphorylation of this inhibitory kinase...
  17. ncbi request reprint Ontogeny of tyrosine phosphorylation-signaling pathways during spermatogenesis and epididymal maturation in the mouse
    Minjie Lin
    Centre for Reproductive Science and Australian Research Council ARC, School of Environmental and Life Science, University of Newcastle, Callaghan, New South Wales 2308, Australia
    Biol Reprod 75:588-97. 2006
    ....
  18. ncbi request reprint Analysis of the mechanism by which calcium negatively regulates the tyrosine phosphorylation cascade associated with sperm capacitation
    Mark A Baker
    The ARC Centre of Excellence in Biotechnology and Development, Reproductive Science Group, School of Environmental and Life Science, and Hunter Medical Research Institute, University of Newcastle, NSW, Australia
    J Cell Sci 117:211-22. 2004
    ....
  19. ncbi request reprint The importance of redox regulated pathways in sperm cell biology
    Mark A Baker
    Discipline of Biological Sciences, School of Environmental and Life Sciences and ARC Centre of Excellence in Biotechnology and Development, The University of Newcastle, Newcastle, NSW 2308, Australia
    Mol Cell Endocrinol 216:47-54. 2004
    ..Given the central importance of redox chemistry in the control of sperm function further research in this area may uncover valuable targets for contraceptive intervention...
  20. ncbi request reprint Proteomic changes in mammalian spermatozoa during epididymal maturation
    R John Aitken
    The ARC Centre of Excellence in Biotechnology and Development, University of Newcastle, Newcastle, NSW 2308, Australia
    Asian J Androl 9:554-64. 2007
    ..At least in rodent species, activation of sperm mitochondria appears to be a particularly important part of epididymal maturation...
  21. doi request reprint The role of proteomics in understanding sperm cell biology
    R John Aitken
    ARC Centre of Excellence in Biotechnology and Development, Discipline of Biological Sciences, University of Newcastle, Callaghan, NSW, Australia
    Int J Androl 31:295-302. 2008
    ....
  22. ncbi request reprint Shedding light on chemiluminescence: the application of chemiluminescence in diagnostic andrology
    R John Aitken
    ARC Centre of Excellence in Biotechnology and Development, Discipline of Biological Sciences, Faculty of Science and IT, University of Newcastle, Callaghan, New South Wales, Australia
    J Androl 25:455-65. 2004
  23. doi request reprint Analysis of proteomic changes associated with sperm capacitation through the combined use of IPG-strip pre-fractionation followed by RP chromatography LC-MS/MS analysis
    Mark A Baker
    Discipline of Biological Sciences, School of Environmental and Life Sciences, University of Newcastle, NSW, Australia
    Proteomics 10:482-95. 2010
    ..To validate our data, we were able to show significant upregulation of SORD activity during capacitation, suggesting that the polyol pathway is activated during this process...
  24. pmc The chaperonin containing TCP1 complex (CCT/TRiC) is involved in mediating sperm-oocyte interaction
    Matthew D Dun
    Reproductive Science Group, School of Environmental and Life Sciences, Discipline of Biological Sciences, University of Newcastle, Callaghan, New South Wales 2308, Australia
    J Biol Chem 286:36875-87. 2011
    ..Collectively, these data provide an important biochemical insight into the molecular basis of sperm-zona pellucida interaction and a plausible explanation for how spermatozoa gain their ability to fertilize...
  25. ncbi request reprint Identification of cytochrome P450-reductase as the enzyme responsible for NADPH-dependent lucigenin and tetrazolium salt reduction in rat epididymal sperm preparations
    Mark A Baker
    ARC Centre of Excellence in Biotechnology and Development, Reproductive Science Group, School of Environmental and Life Sciences, and Hunter Medical Research Institute, University of Newcastle, Callaghan, New South Wales 2308, Australia
    Biol Reprod 71:307-18. 2004
    ..These results emphasize the importance of the direct NADPH-dependent reduction of superoxide-sensitive probes by cytochrome P450-reductase even though this enzyme does not, on its own accord, produce reactive oxygen species...
  26. ncbi request reprint Oxidative stress in the male germ line and its role in the aetiology of male infertility and genetic disease
    R John Aitken
    ARC Centre of Excellence in Biotechnology and Development, Discipline of Biological Sciences, School of Environmental and Life Sciences, University of Newcastle, NSW 2308, Australia
    Reprod Biomed Online 7:65-70. 2003
    ..Such information could make an important contribution to understanding of the origins of both male infertility and a variety of pathological conditions that affect humans, including cancer and dominant genetic disease...
  27. ncbi request reprint Development of the signalling pathways associated with sperm capacitation during epididymal maturation
    Mark A Baker
    Discipline of Biological Sciences, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW 2308, Australia
    Mol Reprod Dev 64:446-57. 2003
    ..These results emphasize the pivotal role played by calcium and HCO(3) (-) in modulating the changes in tyrosine phosphorylation observed during epididymal maturation...
  28. pmc The molecular chaperone HSPA2 plays a key role in regulating the expression of sperm surface receptors that mediate sperm-egg recognition
    Kate A Redgrove
    Priority Research Centre in Reproductive Science, School of Environmental and Life Sciences, Discipline of Biological Sciences, The University of Newcastle, Callaghan, New South Wales, Australia
    PLoS ONE 7:e50851. 2012
    ....
  29. ncbi request reprint Oxidative stress and male reproductive biology
    R John Aitken
    ARC Centre of Excellence in Biotechnology and Development and Reproductive Science Group, Discipline of Biological Sciences, Faculty of Science and IT, University of Newcastle, Callaghan, NSW, Australia
    Reprod Fertil Dev 16:581-8. 2004
    ....
  30. ncbi request reprint Oxidative stress, sperm survival and fertility control
    R John Aitken
    ARC Centre of Excellence in Biotechnology and Development, Discipline of Biological Sciences, University of Newcastle, NSW 2308, Australia
    Mol Cell Endocrinol 250:66-9. 2006
    ....
  31. pmc Electrophilic aldehydes generated by sperm metabolism activate mitochondrial reactive oxygen species generation and apoptosis by targeting succinate dehydrogenase
    R John Aitken
    Priority Research Centre in Reproductive Science, Discipline of Biological Sciences, Faculty of Science and IT, University of Newcastle, University Drive, Callaghan, New South Wales 2308, Australia
    J Biol Chem 287:33048-60. 2012
    ....
  32. doi request reprint Identification of gene products present in Triton X-100 soluble and insoluble fractions of human spermatozoa lysates using LC-MS/MS analysis
    Mark A Baker
    The ARC Centre of Excellence in Biotechnology and Development, Reproductive Science Group, School of Environmental and Life Sciences, University of Newcastle, Newcastle, Australia
    Proteomics Clin Appl 1:524-32. 2007
    ..This is the first published list of identified proteins in human spermatozoa using LC-MS/MS analysis...
  33. ncbi request reprint Biochemical entities involved in reactive oxygen species generation by human spermatozoa
    Mark A Baker
    Discipline of Biological Sciences and Hunter Medical Research Institute, University of Newcastle, Callaghan, New South Wales, Australia
    Protoplasma 221:145-51. 2003
    ..Understanding the involvement of these enzymes in sperm physiology is essential if we are to understand the causes of oxidative stress in the male germ line...
  34. doi request reprint Causes and consequences of apoptosis in spermatozoa; contributions to infertility and impacts on development
    R John Aitken
    Discipline of Biological Sciences and Priority Research Centre in Reproductive Science, Faculty of Science and IT, University of Newcastle, Australia
    Int J Dev Biol 57:265-72. 2013
    ..These findings have practical implications for the approaches that might be used to detect and prevent DNA damage in spermatozoa...
  35. ncbi request reprint Characterization of VDAC1 as a plasma membrane NADH-oxidoreductase
    Mark A Baker
    ARC Centre of Excellence in Biotechnology and Development, Reproductive Science Group, School of Environmental and Life Science, University of Newcastle, NSW, Australia
    Biofactors 21:215-21. 2004
    ..However, this activity can be inhibited by thiol chelators, suggesting that at least one of the two cysteine groups present in VDAC1 are critical for electron transfer. We propose a model on how electron transport may occur in VDAC1...
  36. pmc As the world grows: contraception in the 21st century
    R John Aitken
    Discipline of Biological Sciences and ARC Centre of Excellence in Biotechnology and Development, University of Newcastle, Callaghan, New South Wales, Australia
    J Clin Invest 118:1330-43. 2008
    ..This Review addresses the contribution that the "omic" revolution can make to the identification of novel contraceptive targets, as well as the progress that has been made for different target molecules under development...
  37. doi request reprint Bridging the gap between male and female fertility control; contraception-on-demand
    R John Aitken
    ARC Centre of Excellence in Biotechnology and Development, Discipline of Biological Sciences, School of Biological and Life Sciences, University of Newcastle, Callaghan, NSW 2308, Australia
    Contraception 78:S28-35. 2008
    ..This review examines the current strategies that are being pursued to achieve this objective...
  38. doi request reprint Oxidative stress and male reproductive health
    Robert J Aitken
    Priority Research Centre in Reproductive Science, Discipline of Biological Sciences, Faculty of Science and IT, University of Newcastle, Callaghan, NSW 2308, Australia
    Asian J Androl 16:31-8. 2014
    ....
  39. doi request reprint Oxidative stress, spermatozoa and leukocytic infiltration: relationships forged by the opposing forces of microbial invasion and the search for perfection
    R John Aitken
    Priority Research Centre in Reproductive Science, Discipline of Biological Sciences, University of Newcastle, NSW 2308, Australia Electronic address
    J Reprod Immunol 100:11-9. 2013
    ....
  40. ncbi request reprint Reactive oxygen species generation by human spermatozoa: a continuing enigma
    R John Aitken
    Int J Androl 25:191-4. 2002
  41. ncbi request reprint VDAC1 is a transplasma membrane NADH-ferricyanide reductase
    Mark A Baker
    Department of Biochemistry and Molecular Biology, School of Biomedical Sciences, Monash University, Building 13D, 100 Wellington Road, Melbourne, Victoria 3800, Australia
    J Biol Chem 279:4811-9. 2004
    ..Our data suggest that a major function of VDAC1 in the plasma membrane is that of a NADH(-ferricyanide) reductase that may be involved in the maintenance of cellular redox homeostasis...