Robert J Aitken

Summary

Affiliation: University of Newcastle
Country: Australia

Publications

  1. doi request reprint Oxidative stress, spermatozoa and leukocytic infiltration: relationships forged by the opposing forces of microbial invasion and the search for perfection
    R John Aitken
    Priority Research Centre in Reproductive Science, Discipline of Biological Sciences, University of Newcastle, NSW 2308, Australia Electronic address
    J Reprod Immunol 100:11-9. 2013
  2. doi request reprint The source and significance of DNA damage in human spermatozoa; a commentary on diagnostic strategies and straw man fallacies
    R J Aitken
    Discipline of Biological Sciences and Priority Research Centre in Reproductive Science, Faculty of Science and IT, University of Newcastle, University Drive, Callaghan, NSW 2308, Australia
    Mol Hum Reprod 19:475-85. 2013
  3. doi request reprint Causes and consequences of apoptosis in spermatozoa; contributions to infertility and impacts on development
    R John Aitken
    Discipline of Biological Sciences and Priority Research Centre in Reproductive Science, Faculty of Science and IT, University of Newcastle, Australia
    Int J Dev Biol 57:265-72. 2013
  4. doi request reprint Sperm motility is lost in vitro as a consequence of mitochondrial free radical production and the generation of electrophilic aldehydes but can be significantly rescued by the presence of nucleophilic thiols
    R John Aitken
    Priority Research Centre for Reproductive Science, Discipline of Biological Sciences, University of Newcastle, Callaghan, New South Wales, Australia
    Biol Reprod 87:110. 2012
  5. doi request reprint On methods for the detection of reactive oxygen species generation by human spermatozoa: analysis of the cellular responses to catechol oestrogen, lipid aldehyde, menadione and arachidonic acid
    R J Aitken
    Discipline of Biological Sciences and Priority Research Centre in Reproductive Science, Faculty of Science and IT, University of Newcastle, Callaghan, NSW, Australia
    Andrology 1:192-205. 2013
  6. doi request reprint Reactive oxygen species and sperm function--in sickness and in health
    R John Aitken
    Priority Research Centre for Reproductive Biology, Faculty of Science, University of Newcastle, Callaghan, NSW 2308, Australia
    J Androl 33:1096-106. 2012
  7. pmc Electrophilic aldehydes generated by sperm metabolism activate mitochondrial reactive oxygen species generation and apoptosis by targeting succinate dehydrogenase
    R John Aitken
    Priority Research Centre in Reproductive Science, Discipline of Biological Sciences, Faculty of Science and IT, University of Newcastle, University Drive, Callaghan, New South Wales 2308, Australia
    J Biol Chem 287:33048-60. 2012
  8. doi request reprint The Simmet lecture: new horizons on an old landscape--oxidative stress, DNA damage and apoptosis in the male germ line
    R J Aitken
    Priority Research Centre for Reproductive Biology, Faculty of Science and IT, University of Newcastle, Callaghan, NSW, Australia
    Reprod Domest Anim 47:7-14. 2012
  9. doi request reprint Redox regulation of human sperm function: from the physiological control of sperm capacitation to the etiology of infertility and DNA damage in the germ line
    Robert J Aitken
    Discipline of Biological Sciences and ARC Centre of Excellence in Biotechnology and Development, School of Environmental and Life Sciences, University of Newcastle, Callaghan, Australia
    Antioxid Redox Signal 14:367-81. 2011
  10. pmc Apoptosis and DNA damage in human spermatozoa
    R John Aitken
    ARC Centre of Excellence in Biotechnology and Development, Priority Research Centre in Reproductive Science, Discipline of Biological Sciences, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW 2308, Australia
    Asian J Androl 13:36-42. 2011

Collaborators

Detail Information

Publications92

  1. doi request reprint Oxidative stress, spermatozoa and leukocytic infiltration: relationships forged by the opposing forces of microbial invasion and the search for perfection
    R John Aitken
    Priority Research Centre in Reproductive Science, Discipline of Biological Sciences, University of Newcastle, NSW 2308, Australia Electronic address
    J Reprod Immunol 100:11-9. 2013
    ....
  2. doi request reprint The source and significance of DNA damage in human spermatozoa; a commentary on diagnostic strategies and straw man fallacies
    R J Aitken
    Discipline of Biological Sciences and Priority Research Centre in Reproductive Science, Faculty of Science and IT, University of Newcastle, University Drive, Callaghan, NSW 2308, Australia
    Mol Hum Reprod 19:475-85. 2013
    ....
  3. doi request reprint Causes and consequences of apoptosis in spermatozoa; contributions to infertility and impacts on development
    R John Aitken
    Discipline of Biological Sciences and Priority Research Centre in Reproductive Science, Faculty of Science and IT, University of Newcastle, Australia
    Int J Dev Biol 57:265-72. 2013
    ..These findings have practical implications for the approaches that might be used to detect and prevent DNA damage in spermatozoa...
  4. doi request reprint Sperm motility is lost in vitro as a consequence of mitochondrial free radical production and the generation of electrophilic aldehydes but can be significantly rescued by the presence of nucleophilic thiols
    R John Aitken
    Priority Research Centre for Reproductive Science, Discipline of Biological Sciences, University of Newcastle, Callaghan, New South Wales, Australia
    Biol Reprod 87:110. 2012
    ..The practical implications of these findings were indicated by the effective preservation of equine spermatozoa for 8 days at ambient temperature when the culture medium was supplemented with penicillamine...
  5. doi request reprint On methods for the detection of reactive oxygen species generation by human spermatozoa: analysis of the cellular responses to catechol oestrogen, lipid aldehyde, menadione and arachidonic acid
    R J Aitken
    Discipline of Biological Sciences and Priority Research Centre in Reproductive Science, Faculty of Science and IT, University of Newcastle, Callaghan, NSW, Australia
    Andrology 1:192-205. 2013
    ....
  6. doi request reprint Reactive oxygen species and sperm function--in sickness and in health
    R John Aitken
    Priority Research Centre for Reproductive Biology, Faculty of Science, University of Newcastle, Callaghan, NSW 2308, Australia
    J Androl 33:1096-106. 2012
    ..Achievement of these objectives will require a systematic evaluation of pro- and antioxidant strategies in vivo and in vitro...
  7. pmc Electrophilic aldehydes generated by sperm metabolism activate mitochondrial reactive oxygen species generation and apoptosis by targeting succinate dehydrogenase
    R John Aitken
    Priority Research Centre in Reproductive Science, Discipline of Biological Sciences, Faculty of Science and IT, University of Newcastle, University Drive, Callaghan, New South Wales 2308, Australia
    J Biol Chem 287:33048-60. 2012
    ....
  8. doi request reprint The Simmet lecture: new horizons on an old landscape--oxidative stress, DNA damage and apoptosis in the male germ line
    R J Aitken
    Priority Research Centre for Reproductive Biology, Faculty of Science and IT, University of Newcastle, Callaghan, NSW, Australia
    Reprod Domest Anim 47:7-14. 2012
    ..Encouragingly, the involvement of oxidative stress in the aetiology of male infertility has opened up new opportunities for therapeutic interventions involving the judicious administration of nucleophiles and other forms of antioxidants...
  9. doi request reprint Redox regulation of human sperm function: from the physiological control of sperm capacitation to the etiology of infertility and DNA damage in the germ line
    Robert J Aitken
    Discipline of Biological Sciences and ARC Centre of Excellence in Biotechnology and Development, School of Environmental and Life Sciences, University of Newcastle, Callaghan, Australia
    Antioxid Redox Signal 14:367-81. 2011
    ..In light of the significance of oxidative stress in the etiology of defective sperm function, a variety of antioxidant therapies are now being assessed for their therapeutic potential...
  10. pmc Apoptosis and DNA damage in human spermatozoa
    R John Aitken
    ARC Centre of Excellence in Biotechnology and Development, Priority Research Centre in Reproductive Science, Discipline of Biological Sciences, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW 2308, Australia
    Asian J Androl 13:36-42. 2011
    ....
  11. doi request reprint Electrophoretic sperm isolation: optimization of electrophoresis conditions and impact on oxidative stress
    R John Aitken
    Discipline of Biological Sciences and Priority Research Centre for Reproductive Science, Faculty of Science and IT, University of Newcastle, University Drive, Callaghan, NSW 2308, Australia
    Hum Reprod 26:1955-64. 2011
    ....
  12. ncbi request reprint Oxidative stress, sperm survival and fertility control
    R John Aitken
    ARC Centre of Excellence in Biotechnology and Development, Discipline of Biological Sciences, University of Newcastle, NSW 2308, Australia
    Mol Cell Endocrinol 250:66-9. 2006
    ....
  13. doi request reprint Apoptosis in the germ line
    R John Aitken
    School of Environmental and Life Sciences, Discipline of Biological Sciences and ARC Centre of Excellence in Biotechnology and Development, University of Newcastle, Callaghan, New South Wales 2308, Australia
    Reproduction 141:139-50. 2011
    ..Our ability to understand and ultimately control the reproductive potential of male and female mammals depends upon a deeper understanding of these fundamental processes...
  14. doi request reprint On the possible origins of DNA damage in human spermatozoa
    R J Aitken
    ARC Centre of Excellence in Biotechnology and Development and Discipline of Biological Sciences, University of Newcastle, Callaghan, NSW, Australia
    Mol Hum Reprod 16:3-13. 2010
    ..Carefully controlled studies are now needed to critically examine this possibility...
  15. doi request reprint Dual purpose contraceptives: targeting fertility and sexually transmitted disease
    R J Aitken
    Discipline of Biological Sciences, Faculty of Science and IT, University of Newcastle, University Drive, Callaghan, NSW 2308, Australia
    J Reprod Immunol 88:228-32. 2011
    ..The further development and refinement of these molecules should permit a radical rethink in the way that safe, effective topical protection is provided to control both fertility and the world-wide spread of STDs...
  16. pmc Gpx5 protects the family jewels
    R John Aitken
    ARC Centre of Excellence in Biotechnology and Development, Discipline of Biological Sciences, University of Newcastle, Newcastle, New South Wales, Australia
    J Clin Invest 119:1849-51. 2009
    ..These results demonstrate the key importance of Gpx5 as an extracellular antioxidant designed to protect maturing mammalian spermatozoa from oxidative stress...
  17. ncbi request reprint Ontogeny of tyrosine phosphorylation-signaling pathways during spermatogenesis and epididymal maturation in the mouse
    Minjie Lin
    Centre for Reproductive Science and Australian Research Council ARC, School of Environmental and Life Science, University of Newcastle, Callaghan, New South Wales 2308, Australia
    Biol Reprod 75:588-97. 2006
    ....
  18. ncbi request reprint Analysis of the mechanism by which calcium negatively regulates the tyrosine phosphorylation cascade associated with sperm capacitation
    Mark A Baker
    The ARC Centre of Excellence in Biotechnology and Development, Reproductive Science Group, School of Environmental and Life Science, and Hunter Medical Research Institute, University of Newcastle, NSW, Australia
    J Cell Sci 117:211-22. 2004
    ....
  19. doi request reprint The presence of a truncated base excision repair pathway in human spermatozoa that is mediated by OGG1
    Tegan B Smith
    Priority Research Centre in Reproductive Science, Discipline of Biological Sciences, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW 2308, Australia
    J Cell Sci 126:1488-97. 2013
    ....
  20. ncbi request reprint Evidence for the involvement of PECAM-1 in a receptor mediated signal-transduction pathway regulating capacitation-associated tyrosine phosphorylation in human spermatozoa
    Brett Nixon
    Reproductive Science Group, University of Newcastle, Callaghan, NSW 2308, Australia
    J Cell Sci 118:4865-77. 2005
    ..Collectively, these data provide the first evidence that a receptor-mediated signal transduction pathway triggers human sperm capacitation and identifies PECAM-1 as the probable initiator of this second messenger cascade...
  21. ncbi request reprint Identification of post-translational modifications that occur during sperm maturation using difference in two-dimensional gel electrophoresis
    Mark A Baker
    The ARC Centre of Excellence in Biotechnology and Development, Reproductive Science Group, School of Environmental and Life Sciences, University of Newcastle, NSW, Australia
    Proteomics 5:1003-12. 2005
    ..We suggest that phosphorylation of this particular protein in a cAMP-dependent manner may contribute to the mechanisms by which motility is conferred upon spermatozoa...
  22. doi request reprint Identification of the molecular chaperone, heat shock protein 1 (chaperonin 10), in the reproductive tract and in capacitating spermatozoa in the male mouse
    Andrew Walsh
    Reproductive Science Group, School of Environmental and Life Sciences, University of Newcastle, Callaghan, New South Wales 2308, Australia
    Biol Reprod 78:983-93. 2008
    ..Collectively, these data invite speculation that a number of molecular chaperones are involved in modification of the sperm surface during capacitation to render these cells functionally competent to engage the process of fertilization...
  23. ncbi request reprint Definitive evidence for the nonmitochondrial production of superoxide anion by human spermatozoa
    Geoffry N De Iuliis
    Centre of Excellence in Biotechnology and Development and Discipline of Biological Sciences, University of Newcastle, Callaghan, New South Wales 2308, Australia
    J Clin Endocrinol Metab 91:1968-75. 2006
    ..However, the source of the free radicals responsible for such damage is still unresolved...
  24. ncbi request reprint Development of the signalling pathways associated with sperm capacitation during epididymal maturation
    Mark A Baker
    Discipline of Biological Sciences, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW 2308, Australia
    Mol Reprod Dev 64:446-57. 2003
    ..These results emphasize the pivotal role played by calcium and HCO(3) (-) in modulating the changes in tyrosine phosphorylation observed during epididymal maturation...
  25. ncbi request reprint Endogenous redox activity in mouse spermatozoa and its role in regulating the tyrosine phosphorylation events associated with sperm capacitation
    Heath W Ecroyd
    Reproductive Science Group, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW 2308, Australia
    Biol Reprod 69:347-54. 2003
    ..Bicarbonate regulated the redox activity of mouse spermatozoa, and this regulation may contribute to the impact of this anion on tyrosine phosphorylation during capacitation of mouse spermatozoa...
  26. doi request reprint Stimulation of mitochondrial reactive oxygen species production by unesterified, unsaturated fatty acids in defective human spermatozoa
    Adam J Koppers
    Discipline of Biological Sciences and ARC Centre of Excellence in Biotechnology and Development, Faculty of Science and IT, University of Newcastle, Callaghan, NSW 2308, Australia
    Free Radic Biol Med 48:112-9. 2010
    ....
  27. ncbi request reprint The identification of mouse sperm-surface-associated proteins and characterization of their ability to act as decapacitation factors
    Brett Nixon
    Reproductive Science Group, School of Environmental and Life Sciences, University of Newcastle, Callaghan, New South Wales 2308, Australia
    Biol Reprod 74:275-87. 2006
    ..Of these proteins, PBP was identified as a primary candidate for a decapacitation factor...
  28. ncbi request reprint Identification of cytochrome P450-reductase as the enzyme responsible for NADPH-dependent lucigenin and tetrazolium salt reduction in rat epididymal sperm preparations
    Mark A Baker
    ARC Centre of Excellence in Biotechnology and Development, Reproductive Science Group, School of Environmental and Life Sciences, and Hunter Medical Research Institute, University of Newcastle, Callaghan, New South Wales 2308, Australia
    Biol Reprod 71:307-18. 2004
    ..These results emphasize the importance of the direct NADPH-dependent reduction of superoxide-sensitive probes by cytochrome P450-reductase even though this enzyme does not, on its own accord, produce reactive oxygen species...
  29. ncbi request reprint The importance of redox regulated pathways in sperm cell biology
    Mark A Baker
    Discipline of Biological Sciences, School of Environmental and Life Sciences and ARC Centre of Excellence in Biotechnology and Development, The University of Newcastle, Newcastle, NSW 2308, Australia
    Mol Cell Endocrinol 216:47-54. 2004
    ..Given the central importance of redox chemistry in the control of sperm function further research in this area may uncover valuable targets for contraceptive intervention...
  30. ncbi request reprint The development of signal transduction pathways during epididymal maturation is calcium dependent
    Heath Ecroyd
    Discipline of Biological Sciences, University of Newcastle, Callaghan, NSW 2308, Australia
    Dev Biol 268:53-63. 2004
    ....
  31. ncbi request reprint Cis-unsaturated fatty acids stimulate reactive oxygen species generation and lipid peroxidation in human spermatozoa
    R John Aitken
    Discipline of Biological Sciences, School of Environmental and Life Sciences, University of Newcastle, Callaghan, New South Wales 2308, Australia
    J Clin Endocrinol Metab 91:4154-63. 2006
    ..This study investigated whether a causative relationship exists between these two attributes of impaired semen quality...
  32. pmc Mobile phone radiation induces reactive oxygen species production and DNA damage in human spermatozoa in vitro
    Geoffry N De Iuliis
    ARC Centre of Excellence in Biotechnology and Development, Callaghan, New South Wales, Australia
    PLoS ONE 4:e6446. 2009
    ..In light of these associations, we have analyzed the influence of RF-EMR on the cell biology of human spermatozoa in vitro...
  33. ncbi request reprint The human spermatozoon--not waving but drowning
    R John Aitken
    Discipline of Biological Sciences, Centre for Life Sciences, University of Newcastle, Callaghan, NSW 2308, Australia
    Adv Exp Med Biol 518:85-98. 2003
    ..Developing animal models with which to establish the validity of these relationships and identifying the environmental factors associated with the proposed 'testicular dysgenesis syndrome' will clearly be important tasks for the future...
  34. ncbi request reprint Tyrosine phosphorylation activates surface chaperones facilitating sperm-zona recognition
    Kelly L Asquith
    Reproductive Science Group, School of Environmental and Life Sciences, University of Newcastle, University Drive, Callaghan, NSW 2308, Australia
    J Cell Sci 117:3645-57. 2004
    ....
  35. ncbi request reprint Identification of RARhoGAP, a novel putative RhoGAP gene expressed in male germ cells
    Benjamin J Curry
    Reproductive Science Group, Discipline of Biological Science, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW 2308, Australia
    Genomics 84:406-18. 2004
    ..We postulate that RARhoGAP may be involved in rearrangements of the cytoskeleton and cell signaling events that occur during spermatogenesis...
  36. doi request reprint Bridging the gap between male and female fertility control; contraception-on-demand
    R John Aitken
    ARC Centre of Excellence in Biotechnology and Development, Discipline of Biological Sciences, School of Biological and Life Sciences, University of Newcastle, Callaghan, NSW 2308, Australia
    Contraception 78:S28-35. 2008
    ..This review examines the current strategies that are being pursued to achieve this objective...
  37. ncbi request reprint Value of DNA integrity assays for fertility evaluation
    R John Aitken
    ARC Centre of Excellence in Biotechnology and Development and Discipline of Biological Sciences, University of Newcastle, NSW 2308, Australia
    Soc Reprod Fertil Suppl 65:81-92. 2007
    ....
  38. doi request reprint The role of proteomics in understanding sperm cell biology
    R John Aitken
    ARC Centre of Excellence in Biotechnology and Development, Discipline of Biological Sciences, University of Newcastle, Callaghan, NSW, Australia
    Int J Androl 31:295-302. 2008
    ....
  39. doi request reprint Composition and significance of detergent resistant membranes in mouse spermatozoa
    Brett Nixon
    Reproductive Science Group, Discipline of Biological Sciences, School of Environmental and Life Sciences, University of Newcastle, Callaghan, New South Wales, Australia
    J Cell Physiol 218:122-34. 2009
    ....
  40. doi request reprint Proteomic insights into spermatozoa: critiques, comments and concerns
    Mark A Baker
    ARC Centre of Excellence in Biotechnology and Development, Discipline of Biological Sciences, School of Environmental and Life Sciences, University of Newcastle, Callaghan, New South Wales 2308, Australia
    Expert Rev Proteomics 6:691-705. 2009
    ..Three comprehensive liquid chromatography-mass spectrometry lists of human, mouse and rat spermatozoa are then compared, insights into the molecular regulation of sperm function discussed and future directions speculated upon...
  41. doi request reprint Label-free quantitation of phosphopeptide changes during rat sperm capacitation
    Mark A Baker
    The ARC Centre of Excellence in Biotechnology and Development, Priority Research Centre in Reproductive Science, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, 2308, Australia
    J Proteome Res 9:718-29. 2010
    ..This change, together with the other protein identifications reported in this study, constitute important new leads in elucidating the biochemical mechanisms by which spermatozoa attain a capacitated state...
  42. ncbi request reprint Oxidative stress and male reproductive biology
    R John Aitken
    ARC Centre of Excellence in Biotechnology and Development and Reproductive Science Group, Discipline of Biological Sciences, Faculty of Science and IT, University of Newcastle, Callaghan, NSW, Australia
    Reprod Fertil Dev 16:581-8. 2004
    ....
  43. ncbi request reprint Localization and significance of molecular chaperones, heat shock protein 1, and tumor rejection antigen gp96 in the male reproductive tract and during capacitation and acrosome reaction
    Kelly L Asquith
    Reproductive Science Group, School of Environmental and Life Sciences, University of Newcastle, Callaghan, New South Wales 2308, Australia
    Biol Reprod 72:328-37. 2005
    ..These data reinforce the notion that these chaperones are intimately involved in the mechanisms by which mammalian spermatozoa both acquire and express their ability to recognize the zona pellucida...
  44. ncbi request reprint Seeds of concern
    R John Aitken
    Discipline of Biological Sciences, School of Environmental and Life Sciences, University of Newcastle, Callaghan, New South Wales 2308, Australia
    Nature 432:48-52. 2004
  45. ncbi request reprint Founders' Lecture. Human spermatozoa: fruits of creation, seeds of doubt
    R John Aitken
    ARC Centre of Excellence in Biotechnology and Development and Reproductive Science Group, Discipline of Biological Sciences, University of Newcastle, NSW, Australia
    Reprod Fertil Dev 16:655-64. 2004
    ..However, the ultimate safeguard against this form of damage will be to understand the biochemical basis of oxidative stress in human spermatozoa, so that the underlying causative mechanisms can be addressed in a logical manner...
  46. ncbi request reprint Identification of cytochrome-b5 reductase as the enzyme responsible for NADH-dependent lucigenin chemiluminescence in human spermatozoa
    Mark A Baker
    The ARC Centre of Excellence in Biotechnology and Development, Reproductive Science Group, School of Environmental and Life Sciences, and Hunter Medical Research Institute, University of Newcastle, Callaghan, New South Wales 2308, Australia
    Biol Reprod 73:334-42. 2005
    ..These results emphasize the importance of the direct NADH-dependent reduction of these putative superoxide-sensitive probes by cytochrome-b5 reductase even though this enzyme does not, on its own accord, produce reactive oxygen species...
  47. doi request reprint Analysis of the relationships between oxidative stress, DNA damage and sperm vitality in a patient population: development of diagnostic criteria
    R John Aitken
    ARC Centre of Excellence in Biotechnology and Development, Discipline of Biological Sciences, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, Australia
    Hum Reprod 25:2415-26. 2010
    ..However, the origin of this damage, its biochemical nature and strategies for its amelioration, still await resolution...
  48. ncbi request reprint Cyclic AMP-dependent tyrosine phosphorylation in tammar wallaby (Macropus eugenii) spermatozoa
    Liga Bennetts
    Discipline of Biological Sciences, The University of Newcastle, NSW 2308, Australia
    J Exp Zool A Comp Exp Biol 301:118-30. 2004
    ..However in Metatherian spermatozoa we could find no evidence that this pathway was redox regulated...
  49. doi request reprint Elucidation of the signaling pathways that underpin capacitation-associated surface phosphotyrosine expression in mouse spermatozoa
    Brett Nixon
    Reproductive Science Group, School of Environmental and Life Sciences, University of Newcastle, Callaghan, Australia
    J Cell Physiol 224:71-83. 2010
    ..Collectively, these data highlight a previously unappreciated role of the ERK module in the modification of the sperm surface during capacitation to render these cells functionally competent to engage in the process of fertilization...
  50. ncbi request reprint Quantitative analysis of gene-specific DNA damage in human spermatozoa
    Dennis E Sawyer
    Center for Biotechnology and Development, Discipline of Biological Sciences, The University of Newcastle, University Drive, Callaghan, NSW 2308, Australia
    Mutat Res 529:21-34. 2003
    ..These studies report, for the first time, quantitative measurements of DNA damage in specific genes of male germ cells, and challenge the commonly held belief that human spermatozoa are particularly vulnerable to DNA damage...
  51. ncbi request reprint Identification and characterization of a novel Mt-retrotransposon highly represented in the female mouse germline
    Janet E Holt
    Reproductive Science Group, Discipline of Biological Science, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW 2308, Australia
    Genomics 87:490-9. 2006
    ..Together with in silico data, we predict Mtfull plays an essential role in folliculogenesis through regulation of gene expression...
  52. ncbi request reprint Oxidative stress in the male germ line and its role in the aetiology of male infertility and genetic disease
    R John Aitken
    ARC Centre of Excellence in Biotechnology and Development, Discipline of Biological Sciences, School of Environmental and Life Sciences, University of Newcastle, NSW 2308, Australia
    Reprod Biomed Online 7:65-70. 2003
    ..Such information could make an important contribution to understanding of the origins of both male infertility and a variety of pathological conditions that affect humans, including cancer and dominant genetic disease...
  53. ncbi request reprint The future of sex
    R John Aitken
    Hunter Medical Research Institute, the Discipline of Biological Sciences, University of Newcastle, Callaghan, New South Wales 2308, Australia
    Nature 415:963. 2002
  54. doi request reprint Biological and clinical significance of DNA damage in the male germ line
    R John Aitken
    ARC Centre of Excellence in Biotechnology and Development, Discipline of Biological Sciences, University of Newcastle, NSW, Australia
    Int J Androl 32:46-56. 2009
    ....
  55. doi request reprint The mouse sperm proteome characterized via IPG strip prefractionation and LC-MS/MS identification
    Mark A Baker
    The ARC Centre of Excellence in Biotechnology and Development, Reproductive Science Group, School of Environmental and Life Sciences, University of Newcastle, NSW, Australia
    Proteomics 8:1720-30. 2008
    ....
  56. ncbi request reprint CXCR4/SDF1 interaction inhibits the primordial to primary follicle transition in the neonatal mouse ovary
    Janet E Holt
    Reproductive Science Group, Discipline of Biological Science, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW 2308, Australia
    Dev Biol 293:449-60. 2006
    ..By retaining follicles in an unactivated state, SDF1/CXCR4 signaling may play an important role in maintaining the size and longevity of the primordial follicle pool...
  57. ncbi request reprint Identification of SRC as a key PKA-stimulated tyrosine kinase involved in the capacitation-associated hyperactivation of murine spermatozoa
    Mark A Baker
    The ARC Centre of Excellence in Biotechnology and Development, Reproductive Science Group, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW 2308, Australia
    J Cell Sci 119:3182-92. 2006
    ..The latter was localized to the acrosome and flagellum of murine spermatozoa by immunocytochemistry, whereas capacitation was associated with an inactivating serine phosphosphorylation of this inhibitory kinase...
  58. doi request reprint The rat sperm proteome characterized via IPG strip prefractionation and LC-MS/MS identification
    Mark A Baker
    The ARC Centre of Excellence in Biotechnology and Development, Reproductive Science Group, School of Environmental and Life Sciences, University of Newcastle, NSW, Australia
    Proteomics 8:2312-21. 2008
    ..This inventory of gene products, the first ever 2-D LC-MS/MS analysis of rat spermatozoa, will be invaluable in directing future research into the molecular mechanisms that drive these highly specialized cells...
  59. ncbi request reprint Analysis of lipid peroxidation in human spermatozoa using BODIPY C11
    R John Aitken
    ARC Centre of Excellence in Biotechnology and Development, Discipline of Biological Sciences, University of Newcastle, NSW, Australia
    Mol Hum Reprod 13:203-11. 2007
    ..We conclude that BODIPY C(11) is an extremely useful probe for indexing peroxidative damage in human spermatozoa...
  60. doi request reprint The biological significance of detergent-resistant membranes in spermatozoa
    Brett Nixon
    Reproductive Science Group, Discipline of Biological Sciences, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, Australia
    J Reprod Immunol 83:8-13. 2009
    ....
  61. pmc Reactive oxygen species in spermatozoa: methods for monitoring and significance for the origins of genetic disease and infertility
    Mark A Baker
    The ARC Centre of Excellence in Biotechnology and Development, Reproductive Science Group, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW 2308, Australia
    Reprod Biol Endocrinol 3:67. 2005
    ..In this review, we consider the possible origins of oxidative damage to human spermatozoa and reflect on the important contribution such stress might make to the origins of genetic disease in our species...
  62. ncbi request reprint Shedding light on chemiluminescence: the application of chemiluminescence in diagnostic andrology
    R John Aitken
    ARC Centre of Excellence in Biotechnology and Development, Discipline of Biological Sciences, Faculty of Science and IT, University of Newcastle, Callaghan, New South Wales, Australia
    J Androl 25:455-65. 2004
  63. ncbi request reprint Redox activity associated with the maturation and capacitation of mammalian spermatozoa
    R John Aitken
    ARC Centre of Excellence in Biotechnology and Development, Discipline of Biological Sciences, University of Newcastle, New South Wales, Australia
    Free Radic Biol Med 36:994-1010. 2004
    ....
  64. ncbi request reprint Origins and consequences of DNA damage in male germ cells
    R John Aitken
    ARC Centre of Excellence in Biotechnology and Development and Discipline of Biological Sciences, University of Newcastle, NSW 2308, Australia
    Reprod Biomed Online 14:727-33. 2007
    ..Ultimately, resolving the precise nature of the DNA lesions present in the spermatozoa of infertile men will be an important step towards uncovering the aetiology of this damage and developing strategies for its clinical management...
  65. ncbi request reprint Proteomic changes in mammalian spermatozoa during epididymal maturation
    R John Aitken
    The ARC Centre of Excellence in Biotechnology and Development, University of Newcastle, Newcastle, NSW 2308, Australia
    Asian J Androl 9:554-64. 2007
    ..At least in rodent species, activation of sperm mitochondria appears to be a particularly important part of epididymal maturation...
  66. doi request reprint Investigation of the role of SRC in capacitation-associated tyrosine phosphorylation of human spermatozoa
    Lisa A Mitchell
    Reproductive Science Group, Discipline of Biological Sciences, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW 2308, Australia
    Mol Hum Reprod 14:235-43. 2008
    ..However, although the inhibition of PKA was also accompanied by a suppression of sperm motility, SRC inhibition did not induce a similar response...
  67. ncbi request reprint Tyrosine phosphorylation of HSP-90 during mammalian sperm capacitation
    Heath Ecroyd
    Reproductive Science Group, School of Environmental and Life Sciences, University of Newcastle, Callaghan, New South Wales 2308, Australia
    Biol Reprod 69:1801-7. 2003
    ....
  68. ncbi request reprint Immunocontraceptive vaccines for human use
    R John Aitken
    Discipline of Biological Sciences, Center for Life Sciences, School of Biological and Chemical Sciences, University of Newcastle, Callaghan, NSW, Australia
    J Reprod Immunol 57:273-87. 2002
    ..However, anti-hCG vaccines have advanced to the stage of clinical trials and appear promising...
  69. doi request reprint Use of titanium dioxide to find phosphopeptide and total protein changes during epididymal sperm maturation
    Mark A Baker
    The ARC Centre of Excellence in Biotechnology and Development, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, 2308, Australia
    J Proteome Res 10:1004-17. 2011
    ..These data provide mechanistic insight into the means by which spermatozoa acquire functionality during epididymal transit...
  70. doi request reprint Oxidative stress and male reproductive health
    Robert J Aitken
    Priority Research Centre in Reproductive Science, Discipline of Biological Sciences, Faculty of Science and IT, University of Newcastle, Callaghan, NSW 2308, Australia
    Asian J Androl 16:31-8. 2014
    ....
  71. ncbi request reprint Biochemical entities involved in reactive oxygen species generation by human spermatozoa
    Mark A Baker
    Discipline of Biological Sciences and Hunter Medical Research Institute, University of Newcastle, Callaghan, New South Wales, Australia
    Protoplasma 221:145-51. 2003
    ..Understanding the involvement of these enzymes in sperm physiology is essential if we are to understand the causes of oxidative stress in the male germ line...
  72. doi request reprint Prolactin exerts a prosurvival effect on human spermatozoa via mechanisms that involve the stimulation of Akt phosphorylation and suppression of caspase activation and capacitation
    Dwi Ari Pujianto
    School of Environmental and Life Sciences, University of Newcastle, University Drive Callaghan, New South Wales 2308, Australia
    Endocrinology 151:1269-79. 2010
    ..These findings have implications for preservation of sperm integrity in vivo and in vitro...
  73. doi request reprint Analysis of proteomic changes associated with sperm capacitation through the combined use of IPG-strip pre-fractionation followed by RP chromatography LC-MS/MS analysis
    Mark A Baker
    Discipline of Biological Sciences, School of Environmental and Life Sciences, University of Newcastle, NSW, Australia
    Proteomics 10:482-95. 2010
    ..To validate our data, we were able to show significant upregulation of SORD activity during capacitation, suggesting that the polyol pathway is activated during this process...
  74. doi request reprint The spermostatic and microbicidal actions of quinones and maleimides: toward a dual-purpose contraceptive agent
    Louise M Hughes
    School of Environmental and Life Sciences, Faculty of Science and IT, University of Newcastle, Callaghan, NSW, Australia
    Mol Pharmacol 76:113-24. 2009
    ..Taken together, these results have identified for the first time a novel set of cellular targets and chemical principles capable of providing simultaneous defense against both fertility and the spread of sexually transmitted disease...
  75. doi request reprint DNA damage in human spermatozoa is highly correlated with the efficiency of chromatin remodeling and the formation of 8-hydroxy-2'-deoxyguanosine, a marker of oxidative stress
    Geoffry N De Iuliis
    ARC Centre of Excellence in Biotechnology and Development and Hunter Medical Research Institute, Discipline of Biological Sciences, University of Newcastle, Callaghan, New South Wales 2308, Australia
    Biol Reprod 81:517-24. 2009
    ..These results clearly highlight the importance of oxidative stress in the induction of sperm DNA damage and carry significant implications for the clinical management of this condition...
  76. doi request reprint Phosphorylation and consequent stimulation of the tyrosine kinase c-Abl by PKA in mouse spermatozoa; its implications during capacitation
    Mark A Baker
    ARC Centre of Excellence in Biotechnology and Development, Reproductive Science Group, School of Environmental and Life Sciences, University of Newcastle, NSW 2308, Australia
    Dev Biol 333:57-66. 2009
    ..We present a model, whereby these kinases may operate together with SRC to ensure optimal levels of tyrosine phosphorylation in the sperm flagellum during the attainment of a capacitated state...
  77. doi request reprint Significance of mitochondrial reactive oxygen species in the generation of oxidative stress in spermatozoa
    Adam J Koppers
    Discipline of Biological Sciences, University of Newcastle Callaghan, New South Wales 2308, Australia
    J Clin Endocrinol Metab 93:3199-207. 2008
    ..Male infertility has been linked with the excessive generation of reactive oxygen species (ROS) by defective spermatozoa. However, the subcellular origins of this activity are unclear...
  78. ncbi request reprint A comparative study of oxidative DNA damage in mammalian spermatozoa
    Liga E Bennetts
    Discipline of Biological Sciences, The University of Newcastle, New South Wales, Australia
    Mol Reprod Dev 71:77-87. 2005
    ....
  79. ncbi request reprint Male reproductive health and the environment
    R John Aitken
    Med J Aust 185:414-5. 2006
  80. ncbi request reprint The contraceptive potential of ZP3 and ZP3 peptides in a primate model
    Margaret Paterson
    MRC Human Reproductive Sciences Unit, Centre for Reproductive Biology, 37, Chalmers Street, Edinburgh, EH3 9ET, UK
    J Reprod Immunol 53:99-107. 2002
    ..Thus considerable research is still required to identify a combination of ZP epitopes that will induce reversible infertility in the absence of any ovarian dysfunction...
  81. ncbi request reprint Cysteine-rich secretory protein 2 binds to mitogen-activated protein kinase kinase kinase 11 in mouse sperm
    Gerard M Gibbs
    Monash Institute of Medical Research, Monash University, Melbourne, Victoria 3168, Australia
    Biol Reprod 77:108-14. 2007
    ..These data raise the possibility that CRISP2 is a MAP3K11-modifying protein or, alternatively, that MAP3K11 acts to phosphorylate CRISP2 during acrosome development...
  82. ncbi request reprint Identification and characterization of a novel splice variant of mouse and rat cytochrome b5/cytochrome b5 reductase
    Benjamin J Curry
    ARC Centre of Excellence for Biotechnology and Development, Discipline of Biological Sciences, School of Environmental and Life Sciences, University of Newcastle, University Drive, Callaghan, NSW 2308, Australia
    Genomics 83:425-38. 2004
    ..We postulate that cb5/cb5rdelta12 may function in a dominant negative fashion, limiting the amount of damage caused by the production of reactive oxygen species by cb5/cb5r...
  83. ncbi request reprint Integrating new tests of sperm genetic integrity into semen analysis: breakout group discussion
    Sally D Perreault
    U S EPA, ORD, NHEERL, Reproductive Toxicology Division, Research Triangle Park, NC 27711, USA
    Adv Exp Med Biol 518:253-68. 2003
  84. ncbi request reprint Reactive oxygen species generation by human spermatozoa: a continuing enigma
    R John Aitken
    Int J Androl 25:191-4. 2002
  85. ncbi request reprint Characterization of structure and expression of the Dzip1 gene in the rat and mouse
    Benjamin J Curry
    Reproductive Science Group, Discipline of Biological Science, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW 2308, Australia
    Genomics 87:275-85. 2006
    ....
  86. ncbi request reprint Contraceptive vaccines
    Eileen A McLaughlin
    ARC Centre of Excellence in Biotechnology and Development, Discipline of Biological Sciences, University of Newcastle, Callaghan NSW2308, Australia
    Expert Opin Biol Ther 3:829-41. 2003
    ....
  87. doi request reprint Need for standardization and confirmation of STS deletions on the Y chromosome
    Robert Ian McLachlan
    Fertil Steril 90:463-4; author reply 464. 2008
  88. ncbi request reprint Biosynthesis of the canine zona pellucida requires the integrated participation of both oocytes and granulosa cells
    Daniel G Blackmore
    Reproductive Science Group, Discipline of Biological Sciences, School of Environmental and Life Sciences, University of Newcastle, Callaghan, New South Wales 2308, Australia
    Biol Reprod 71:661-8. 2004
    ....
  89. ncbi request reprint Vitamin A regulation of BMP4 expression in the male germ line
    Rosa M Baleato
    Reproductive Science Group, School of Environmental and Life Sciences, University of Newcastle, NSW 2308, Australia
    Dev Biol 286:78-90. 2005
    ..Our results reflect a direct requirement for retinoids by germ cells for the resumption of spermatogenesis in VAD animals via mechanisms that involve the suppression of BMP4 expression...
  90. ncbi request reprint Polyunsaturated fatty acids in male and female reproduction
    D Claire Wathes
    Department of Veterinary Basic Sciences, Royal Veterinary College, North Mymms, Hatfield, Herts, AL9 7TA, United Kingdom
    Biol Reprod 77:190-201. 2007
    ..Adequately powered trials that control for the ratios of different PUFAs consumed are required to determine the extent to which this aspect of our diets does influence our fertility...
  91. ncbi request reprint Round table discussion on "Public health: contribution of family planning towards public health"
    Ursula F Habenicht
    Schering AG, CRBA Gynecology and Andrology, Berlin, Germany
    Mol Cell Endocrinol 250:212-3. 2006
  92. pmc As the world grows: contraception in the 21st century
    R John Aitken
    Discipline of Biological Sciences and ARC Centre of Excellence in Biotechnology and Development, University of Newcastle, Callaghan, New South Wales, Australia
    J Clin Invest 118:1330-43. 2008
    ..This Review addresses the contribution that the "omic" revolution can make to the identification of novel contraceptive targets, as well as the progress that has been made for different target molecules under development...