R J Aitken

Summary

Affiliation: University of Newcastle
Country: Australia

Publications

  1. ncbi request reprint Shedding light on chemiluminescence: the application of chemiluminescence in diagnostic andrology
    R John Aitken
    ARC Centre of Excellence in Biotechnology and Development, Discipline of Biological Sciences, Faculty of Science and IT, University of Newcastle, Callaghan, New South Wales, Australia
    J Androl 25:455-65. 2004
  2. ncbi request reprint Oxidative stress, sperm survival and fertility control
    R John Aitken
    ARC Centre of Excellence in Biotechnology and Development, Discipline of Biological Sciences, University of Newcastle, NSW 2308, Australia
    Mol Cell Endocrinol 250:66-9. 2006
  3. pmc Reactive oxygen species in spermatozoa: methods for monitoring and significance for the origins of genetic disease and infertility
    Mark A Baker
    The ARC Centre of Excellence in Biotechnology and Development, Reproductive Science Group, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW 2308, Australia
    Reprod Biol Endocrinol 3:67. 2005
  4. ncbi request reprint Sperm function tests and fertility
    R J Aitken
    ARC Centre of Excellence in Biotechnology and Development, Discipline of Biological Sciences, and Hunter Medical Research Institute, University of Newcastle, Callaghan, NSW, Australia
    Int J Androl 29:69-75; discussion 105-8. 2006
  5. ncbi request reprint Oxidative stress in the male germ line and its role in the aetiology of male infertility and genetic disease
    R John Aitken
    ARC Centre of Excellence in Biotechnology and Development, Discipline of Biological Sciences, School of Environmental and Life Sciences, University of Newcastle, NSW 2308, Australia
    Reprod Biomed Online 7:65-70. 2003
  6. ncbi request reprint Multiple forms of redox activity in populations of human spermatozoa
    R J Aitken
    ARC Centre of Excellence in Biotechnology and Development and Discipline of Biological Sciences, School of Environmental and Life Sciences, Faculty of Science and Information Technology, University of Newcastle, Australia
    Mol Hum Reprod 9:645-61. 2003
  7. doi request reprint The role of proteomics in understanding sperm cell biology
    R John Aitken
    ARC Centre of Excellence in Biotechnology and Development, Discipline of Biological Sciences, University of Newcastle, Callaghan, NSW, Australia
    Int J Androl 31:295-302. 2008
  8. ncbi request reprint Redox activity associated with the maturation and capacitation of mammalian spermatozoa
    R John Aitken
    ARC Centre of Excellence in Biotechnology and Development, Discipline of Biological Sciences, University of Newcastle, New South Wales, Australia
    Free Radic Biol Med 36:994-1010. 2004
  9. ncbi request reprint Value of DNA integrity assays for fertility evaluation
    R John Aitken
    ARC Centre of Excellence in Biotechnology and Development and Discipline of Biological Sciences, University of Newcastle, NSW 2308, Australia
    Soc Reprod Fertil Suppl 65:81-92. 2007
  10. ncbi request reprint Proteomic changes in mammalian spermatozoa during epididymal maturation
    R John Aitken
    The ARC Centre of Excellence in Biotechnology and Development, University of Newcastle, Newcastle, NSW 2308, Australia
    Asian J Androl 9:554-64. 2007

Collaborators

Detail Information

Publications107 found, 100 shown here

  1. ncbi request reprint Shedding light on chemiluminescence: the application of chemiluminescence in diagnostic andrology
    R John Aitken
    ARC Centre of Excellence in Biotechnology and Development, Discipline of Biological Sciences, Faculty of Science and IT, University of Newcastle, Callaghan, New South Wales, Australia
    J Androl 25:455-65. 2004
  2. ncbi request reprint Oxidative stress, sperm survival and fertility control
    R John Aitken
    ARC Centre of Excellence in Biotechnology and Development, Discipline of Biological Sciences, University of Newcastle, NSW 2308, Australia
    Mol Cell Endocrinol 250:66-9. 2006
    ....
  3. pmc Reactive oxygen species in spermatozoa: methods for monitoring and significance for the origins of genetic disease and infertility
    Mark A Baker
    The ARC Centre of Excellence in Biotechnology and Development, Reproductive Science Group, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW 2308, Australia
    Reprod Biol Endocrinol 3:67. 2005
    ..In this review, we consider the possible origins of oxidative damage to human spermatozoa and reflect on the important contribution such stress might make to the origins of genetic disease in our species...
  4. ncbi request reprint Sperm function tests and fertility
    R J Aitken
    ARC Centre of Excellence in Biotechnology and Development, Discipline of Biological Sciences, and Hunter Medical Research Institute, University of Newcastle, Callaghan, NSW, Australia
    Int J Androl 29:69-75; discussion 105-8. 2006
    ..Such assessments not only yield information on the fertilizing capacity of human spermatozoa but also their ability to support normal embryonic development...
  5. ncbi request reprint Oxidative stress in the male germ line and its role in the aetiology of male infertility and genetic disease
    R John Aitken
    ARC Centre of Excellence in Biotechnology and Development, Discipline of Biological Sciences, School of Environmental and Life Sciences, University of Newcastle, NSW 2308, Australia
    Reprod Biomed Online 7:65-70. 2003
    ..Such information could make an important contribution to understanding of the origins of both male infertility and a variety of pathological conditions that affect humans, including cancer and dominant genetic disease...
  6. ncbi request reprint Multiple forms of redox activity in populations of human spermatozoa
    R J Aitken
    ARC Centre of Excellence in Biotechnology and Development and Discipline of Biological Sciences, School of Environmental and Life Sciences, Faculty of Science and Information Technology, University of Newcastle, Australia
    Mol Hum Reprod 9:645-61. 2003
    ..Their distinct pharmacological profiles should significantly assist attempts to resolve and characterize these systems...
  7. doi request reprint The role of proteomics in understanding sperm cell biology
    R John Aitken
    ARC Centre of Excellence in Biotechnology and Development, Discipline of Biological Sciences, University of Newcastle, Callaghan, NSW, Australia
    Int J Androl 31:295-302. 2008
    ....
  8. ncbi request reprint Redox activity associated with the maturation and capacitation of mammalian spermatozoa
    R John Aitken
    ARC Centre of Excellence in Biotechnology and Development, Discipline of Biological Sciences, University of Newcastle, New South Wales, Australia
    Free Radic Biol Med 36:994-1010. 2004
    ....
  9. ncbi request reprint Value of DNA integrity assays for fertility evaluation
    R John Aitken
    ARC Centre of Excellence in Biotechnology and Development and Discipline of Biological Sciences, University of Newcastle, NSW 2308, Australia
    Soc Reprod Fertil Suppl 65:81-92. 2007
    ....
  10. ncbi request reprint Proteomic changes in mammalian spermatozoa during epididymal maturation
    R John Aitken
    The ARC Centre of Excellence in Biotechnology and Development, University of Newcastle, Newcastle, NSW 2308, Australia
    Asian J Androl 9:554-64. 2007
    ..At least in rodent species, activation of sperm mitochondria appears to be a particularly important part of epididymal maturation...
  11. ncbi request reprint Oxidative stress and male reproductive biology
    R John Aitken
    ARC Centre of Excellence in Biotechnology and Development and Reproductive Science Group, Discipline of Biological Sciences, Faculty of Science and IT, University of Newcastle, Callaghan, NSW, Australia
    Reprod Fertil Dev 16:581-8. 2004
    ....
  12. ncbi request reprint Molecular mechanisms of sperm capacitation: progesterone-induced secondary calcium oscillations reflect the attainment of a capacitated state
    R J Aitken
    RC Centre of Excellence in Biotechnology and Development, Discipline of Biological Sciences, University of Newcastle, Callaghan, NSW 2708, Australia
    Soc Reprod Fertil Suppl 63:273-93. 2007
    ..The secondary calcium oscillations created via these channels do not induce acrosomal exocytosis per se but may prime the cells so that this event is rapidly triggered when the spermatozoa make contact with the zona pellucida...
  13. ncbi request reprint Seeds of concern
    R John Aitken
    Discipline of Biological Sciences, School of Environmental and Life Sciences, University of Newcastle, Callaghan, New South Wales 2308, Australia
    Nature 432:48-52. 2004
  14. ncbi request reprint Origins and consequences of DNA damage in male germ cells
    R John Aitken
    ARC Centre of Excellence in Biotechnology and Development and Discipline of Biological Sciences, University of Newcastle, NSW 2308, Australia
    Reprod Biomed Online 14:727-33. 2007
    ..Ultimately, resolving the precise nature of the DNA lesions present in the spermatozoa of infertile men will be an important step towards uncovering the aetiology of this damage and developing strategies for its clinical management...
  15. ncbi request reprint Founders' Lecture. Human spermatozoa: fruits of creation, seeds of doubt
    R John Aitken
    ARC Centre of Excellence in Biotechnology and Development and Reproductive Science Group, Discipline of Biological Sciences, University of Newcastle, NSW, Australia
    Reprod Fertil Dev 16:655-64. 2004
    ..However, the ultimate safeguard against this form of damage will be to understand the biochemical basis of oxidative stress in human spermatozoa, so that the underlying causative mechanisms can be addressed in a logical manner...
  16. ncbi request reprint Impact of radio frequency electromagnetic radiation on DNA integrity in the male germline
    R J Aitken
    ARC Centre of Excellence in Biotechnology and Development, Discipline of Biological Sciences, and Hunter Medical Research Institute, Newcastle, NSW, Australia
    Int J Androl 28:171-9. 2005
    ..01). This study suggests that while RFEMR does not have a dramatic impact on male germ cell development, a significant genotoxic effect on epididymal spermatozoa is evident and deserves further investigation...
  17. ncbi request reprint Analysis of lipid peroxidation in human spermatozoa using BODIPY C11
    R John Aitken
    ARC Centre of Excellence in Biotechnology and Development, Discipline of Biological Sciences, University of Newcastle, NSW, Australia
    Mol Hum Reprod 13:203-11. 2007
    ..We conclude that BODIPY C(11) is an extremely useful probe for indexing peroxidative damage in human spermatozoa...
  18. ncbi request reprint Cis-unsaturated fatty acids stimulate reactive oxygen species generation and lipid peroxidation in human spermatozoa
    R John Aitken
    Discipline of Biological Sciences, School of Environmental and Life Sciences, University of Newcastle, Callaghan, New South Wales 2308, Australia
    J Clin Endocrinol Metab 91:4154-63. 2006
    ..This study investigated whether a causative relationship exists between these two attributes of impaired semen quality...
  19. doi request reprint Bridging the gap between male and female fertility control; contraception-on-demand
    R John Aitken
    ARC Centre of Excellence in Biotechnology and Development, Discipline of Biological Sciences, School of Biological and Life Sciences, University of Newcastle, Callaghan, NSW 2308, Australia
    Contraception 78:S28-35. 2008
    ..This review examines the current strategies that are being pursued to achieve this objective...
  20. doi request reprint Biological and clinical significance of DNA damage in the male germ line
    R John Aitken
    ARC Centre of Excellence in Biotechnology and Development, Discipline of Biological Sciences, University of Newcastle, NSW, Australia
    Int J Androl 32:46-56. 2009
    ....
  21. ncbi request reprint The human spermatozoon--not waving but drowning
    R John Aitken
    Discipline of Biological Sciences, Centre for Life Sciences, University of Newcastle, Callaghan, NSW 2308, Australia
    Adv Exp Med Biol 518:85-98. 2003
    ..Developing animal models with which to establish the validity of these relationships and identifying the environmental factors associated with the proposed 'testicular dysgenesis syndrome' will clearly be important tasks for the future...
  22. ncbi request reprint Oxidative stress, DNA damage and the Y chromosome
    R J Aitken
    School of Biological and Chemical Sciences, Centre for Life Sciences, University of Newcastle, University Drive, Callaghan, NSW 23008, Australia
    Reproduction 122:497-506. 2001
    ....
  23. doi request reprint Analysis of the relationships between oxidative stress, DNA damage and sperm vitality in a patient population: development of diagnostic criteria
    R John Aitken
    ARC Centre of Excellence in Biotechnology and Development, Discipline of Biological Sciences, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, Australia
    Hum Reprod 25:2415-26. 2010
    ..However, the origin of this damage, its biochemical nature and strategies for its amelioration, still await resolution...
  24. ncbi request reprint Impact of a deep saturation dive on semen quality
    R J Aitken
    MRC Reproductive Biology Unit, 37 Chalmers Street, Edinburgh EH3 9ET, Scotland
    Int J Androl 23:116-20. 2000
    ..These studies indicate, for the first time, that the severe hyperbaric conditions associated with deep saturation dives have a profound effect on male reproductive function...
  25. ncbi request reprint Immunocontraceptive vaccines for human use
    R John Aitken
    Discipline of Biological Sciences, Center for Life Sciences, School of Biological and Chemical Sciences, University of Newcastle, Callaghan, NSW, Australia
    J Reprod Immunol 57:273-87. 2002
    ..However, anti-hCG vaccines have advanced to the stage of clinical trials and appear promising...
  26. ncbi request reprint A redox-regulated tyrosine phosphorylation cascade in rat spermatozoa
    B Lewis
    MRC Reproductive Biology Unit, Centre for Reproductive Biology, University of Edinburgh, Scotland
    J Androl 22:611-22. 2001
    ....
  27. ncbi request reprint On the cellular mechanisms by which the bicarbonate ion mediates the extragenomic action of progesterone on human spermatozoa
    R J Aitken
    MRC Reproductive Biology Unit, Edinburgh, Scotland
    Biol Reprod 58:186-96. 1998
    ....
  28. doi request reprint On methods for the detection of reactive oxygen species generation by human spermatozoa: analysis of the cellular responses to catechol oestrogen, lipid aldehyde, menadione and arachidonic acid
    R J Aitken
    Discipline of Biological Sciences and Priority Research Centre in Reproductive Science, Faculty of Science and IT, University of Newcastle, Callaghan, NSW, Australia
    Andrology 1:192-205. 2013
    ....
  29. ncbi request reprint Adenylyl cyclase isoforms in rat testis and spermatozoa from the cauda epididymidis
    M A Wade
    Reproductive Science Group, ARC Centre of Excellence in Biotechnology and Development, School of Environmental and Life Sciences, University of Newcastle, NSW 2308, Australia
    Cell Tissue Res 314:411-9. 2003
    ..These findings provide a fundamental basis for future studies, to determine the physiological roles of tmAC in testis and mature spermatozoa...
  30. doi request reprint Impact of estrogenic compounds on DNA integrity in human spermatozoa: evidence for cross-linking and redox cycling activities
    L E Bennetts
    ARC Centre of Excellence in Biotechnology and Development and Discipline of Biological Sciences, University of Newcastle, NSW, Australia
    Mutat Res 641:1-11. 2008
    ....
  31. ncbi request reprint Analysis of chaperone proteins associated with human spermatozoa during capacitation
    L A Mitchell
    Reproductive Science Group, Discipline of Biological Sciences, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, Australia
    Mol Hum Reprod 13:605-13. 2007
    ..We conclude that strong species-specific differences exist in the molecular mechanisms that drive sperm-egg recognition and that alternative, chaperone-independent, mechanisms must underpin sperm-zona interaction in the human...
  32. ncbi request reprint Analysis of reactive oxygen species generating systems in rat epididymal spermatozoa
    P Vernet
    MRC Reproductive Biology Unit, Edinburgh EH3 9ET, Scotland, UK
    Biol Reprod 65:1102-13. 2001
    ..Reverse transcription-polymerase chain reaction analysis indicated that the sperm NAD(P)H oxidoreductase complex is quite distinct from the equivalent leukocyte system...
  33. ncbi request reprint New insights into the molecular mechanisms of sperm-egg interaction
    B Nixon
    Reproductive Science Group, ARC Centre of Excellence in Biotechnology and Development, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW 2308, Australia
    Cell Mol Life Sci 64:1805-23. 2007
    ..Herein, we describe two models indicating the plethora of highly orchestrated molecular interactions underlying successful sperm zona binding and sperm oocyte fusion...
  34. doi request reprint Prospective controlled trial of an electrophoretic method of sperm preparation for assisted reproduction: comparison with density gradient centrifugation
    S D Fleming
    Department of Obstetrics and Gynaecology, Westmead Fertility Centre, Westmead Hospital, Sydney, NSW, Australia
    Hum Reprod 23:2646-51. 2008
    ..However, it remains to be proven whether spermatozoa prepared in this way are capable of achieving fertilization in assisted conception. Therefore, this clinical trial was designed to answer this question...
  35. doi request reprint The electrophoretic separation of spermatozoa: an analysis of genotype, surface carbohydrate composition and potential for capacitation
    C J Ainsworth
    Discipline of Biological Sciences and Priority Research Centre in Reproductive Science, Faculty of Science and IT, University of Newcastle, University Drive, Callaghan NSW, Australia
    Int J Androl 34:e422-34. 2011
    ....
  36. ncbi request reprint Analysis of the mechanism by which calcium negatively regulates the tyrosine phosphorylation cascade associated with sperm capacitation
    Mark A Baker
    The ARC Centre of Excellence in Biotechnology and Development, Reproductive Science Group, School of Environmental and Life Science, and Hunter Medical Research Institute, University of Newcastle, NSW, Australia
    J Cell Sci 117:211-22. 2004
    ....
  37. ncbi request reprint Ontogeny of tyrosine phosphorylation-signaling pathways during spermatogenesis and epididymal maturation in the mouse
    Minjie Lin
    Centre for Reproductive Science and Australian Research Council ARC, School of Environmental and Life Science, University of Newcastle, Callaghan, New South Wales 2308, Australia
    Biol Reprod 75:588-97. 2006
    ....
  38. ncbi request reprint Relative susceptibilities of mitochondrial and nuclear DNA to damage induced by hydrogen peroxide in two mouse germ cell lines
    D E Sawyer
    Discipline of Biological Sciences, The University of Newcastle, Callaghan, New South Wales, Australia
    Redox Rep 6:182-4. 2001
    ..These results demonstrate that the nuclear DNA in these germ cell lines is relatively resistant to peroxide-mediated DNA damage, and that mitochondrial DNA is a sensitive biomarker for oxidative stress in these cells...
  39. ncbi request reprint Development of a novel electrophoretic system for the isolation of human spermatozoa
    C Ainsworth
    Discipline of Biological Sciences and ARC Centre of Excellence in Biotechnology and Development, University of Newcastle, NSW 2308, Australia
    Hum Reprod 20:2261-70. 2005
    ..In this study we describe a novel electrophoretic sperm isolation technique that achieves these objectives...
  40. ncbi request reprint The search for a topical dual action spermicide/microbicide
    Louise M Hughes
    Discipline of Biological Sciences School of Environmental and Life Sciences, University of Newcastle, Callaghan NSW 2308, Australia
    Curr Med Chem 14:775-86. 2007
    ..The chemical principles underlying these disparate approaches and potential avenues for future investigation are discussed...
  41. ncbi request reprint Motility activation and second messenger signalling in spermatozoa from rat cauda epididymidis
    M A Wade
    Discipline of Biological Sciences, School of Environmental and Life Sciences, University of Newcastle, NSW, 2308, Australia
    Reproduction 125:175-83. 2003
    ....
  42. ncbi request reprint Endogenous redox activity in mouse spermatozoa and its role in regulating the tyrosine phosphorylation events associated with sperm capacitation
    Heath W Ecroyd
    Reproductive Science Group, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW 2308, Australia
    Biol Reprod 69:347-54. 2003
    ..Bicarbonate regulated the redox activity of mouse spermatozoa, and this regulation may contribute to the impact of this anion on tyrosine phosphorylation during capacitation of mouse spermatozoa...
  43. ncbi request reprint Identification of post-translational modifications that occur during sperm maturation using difference in two-dimensional gel electrophoresis
    Mark A Baker
    The ARC Centre of Excellence in Biotechnology and Development, Reproductive Science Group, School of Environmental and Life Sciences, University of Newcastle, NSW, Australia
    Proteomics 5:1003-12. 2005
    ..We suggest that phosphorylation of this particular protein in a cAMP-dependent manner may contribute to the mechanisms by which motility is conferred upon spermatozoa...
  44. ncbi request reprint Development of the signalling pathways associated with sperm capacitation during epididymal maturation
    Mark A Baker
    Discipline of Biological Sciences, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW 2308, Australia
    Mol Reprod Dev 64:446-57. 2003
    ..These results emphasize the pivotal role played by calcium and HCO(3) (-) in modulating the changes in tyrosine phosphorylation observed during epididymal maturation...
  45. ncbi request reprint Definitive evidence for the nonmitochondrial production of superoxide anion by human spermatozoa
    Geoffry N De Iuliis
    Centre of Excellence in Biotechnology and Development and Discipline of Biological Sciences, University of Newcastle, Callaghan, New South Wales 2308, Australia
    J Clin Endocrinol Metab 91:1968-75. 2006
    ..However, the source of the free radicals responsible for such damage is still unresolved...
  46. ncbi request reprint Evidence for the involvement of PECAM-1 in a receptor mediated signal-transduction pathway regulating capacitation-associated tyrosine phosphorylation in human spermatozoa
    Brett Nixon
    Reproductive Science Group, University of Newcastle, Callaghan, NSW 2308, Australia
    J Cell Sci 118:4865-77. 2005
    ..Collectively, these data provide the first evidence that a receptor-mediated signal transduction pathway triggers human sperm capacitation and identifies PECAM-1 as the probable initiator of this second messenger cascade...
  47. doi request reprint Identification of the molecular chaperone, heat shock protein 1 (chaperonin 10), in the reproductive tract and in capacitating spermatozoa in the male mouse
    Andrew Walsh
    Reproductive Science Group, School of Environmental and Life Sciences, University of Newcastle, Callaghan, New South Wales 2308, Australia
    Biol Reprod 78:983-93. 2008
    ..Collectively, these data invite speculation that a number of molecular chaperones are involved in modification of the sperm surface during capacitation to render these cells functionally competent to engage the process of fertilization...
  48. doi request reprint The simmet lecture: new horizons on an old landscape--oxidative stress, DNA damage and apoptosis in the male germ line
    R J Aitken
    Priority Research Centre for Reproductive Biology, Faculty of Science and IT, University of Newcastle, Callaghan, NSW, Australia
    Reprod Domest Anim 47:7-14. 2012
    ..Encouragingly, the involvement of oxidative stress in the aetiology of male infertility has opened up new opportunities for therapeutic interventions involving the judicious administration of nucleophiles and other forms of antioxidants...
  49. ncbi request reprint First recorded pregnancy and normal birth after ICSI using electrophoretically isolated spermatozoa
    C Ainsworth
    Reproductive Science Group, Discipline of Biological Sciences, University of Newcastle, Callaghan, NSW, Australia
    Hum Reprod 22:197-200. 2007
    ..Given these associations, embryologists are keen to develop techniques that will allow the selection of viable spermatozoa exhibiting low levels of DNA damage for assisted conception purposes...
  50. ncbi request reprint Identification of cytochrome P450-reductase as the enzyme responsible for NADPH-dependent lucigenin and tetrazolium salt reduction in rat epididymal sperm preparations
    Mark A Baker
    ARC Centre of Excellence in Biotechnology and Development, Reproductive Science Group, School of Environmental and Life Sciences, and Hunter Medical Research Institute, University of Newcastle, Callaghan, New South Wales 2308, Australia
    Biol Reprod 71:307-18. 2004
    ..These results emphasize the importance of the direct NADPH-dependent reduction of superoxide-sensitive probes by cytochrome P450-reductase even though this enzyme does not, on its own accord, produce reactive oxygen species...
  51. ncbi request reprint The importance of redox regulated pathways in sperm cell biology
    Mark A Baker
    Discipline of Biological Sciences, School of Environmental and Life Sciences and ARC Centre of Excellence in Biotechnology and Development, The University of Newcastle, Newcastle, NSW 2308, Australia
    Mol Cell Endocrinol 216:47-54. 2004
    ..Given the central importance of redox chemistry in the control of sperm function further research in this area may uncover valuable targets for contraceptive intervention...
  52. ncbi request reprint The development of signal transduction pathways during epididymal maturation is calcium dependent
    Heath Ecroyd
    Discipline of Biological Sciences, University of Newcastle, Callaghan, NSW 2308, Australia
    Dev Biol 268:53-63. 2004
    ....
  53. ncbi request reprint The identification of mouse sperm-surface-associated proteins and characterization of their ability to act as decapacitation factors
    Brett Nixon
    Reproductive Science Group, School of Environmental and Life Sciences, University of Newcastle, Callaghan, New South Wales 2308, Australia
    Biol Reprod 74:275-87. 2006
    ..Of these proteins, PBP was identified as a primary candidate for a decapacitation factor...
  54. doi request reprint Dual purpose contraceptives: targeting fertility and sexually transmitted disease
    R J Aitken
    Discipline of Biological Sciences, Faculty of Science and IT, University of Newcastle, University Drive, Callaghan, NSW 2308, Australia
    J Reprod Immunol 88:228-32. 2011
    ..The further development and refinement of these molecules should permit a radical rethink in the way that safe, effective topical protection is provided to control both fertility and the world-wide spread of STDs...
  55. doi request reprint On the possible origins of DNA damage in human spermatozoa
    R J Aitken
    ARC Centre of Excellence in Biotechnology and Development and Discipline of Biological Sciences, University of Newcastle, Callaghan, NSW, Australia
    Mol Hum Reprod 16:3-13. 2010
    ..Carefully controlled studies are now needed to critically examine this possibility...
  56. ncbi request reprint The extragenomic action of progesterone on human spermatozoa: evidence for a ubiquitous response that is rapidly down-regulated
    R J Aitken
    MRC Reproductive Biology Unit, Edinburgh, Scotland
    Endocrinology 137:3999-4009. 1996
    ..There are implications in these results for our understanding of the extragenomic action of progesterone on human spermatozoa and the clinical manipulation of this system for the assessment and suppression of human sperm function...
  57. doi request reprint Elucidation of the signaling pathways that underpin capacitation-associated surface phosphotyrosine expression in mouse spermatozoa
    Brett Nixon
    Reproductive Science Group, School of Environmental and Life Sciences, University of Newcastle, Callaghan, Australia
    J Cell Physiol 224:71-83. 2010
    ..Collectively, these data highlight a previously unappreciated role of the ERK module in the modification of the sperm surface during capacitation to render these cells functionally competent to engage in the process of fertilization...
  58. ncbi request reprint Cyclic AMP-dependent tyrosine phosphorylation in tammar wallaby (Macropus eugenii) spermatozoa
    Liga Bennetts
    Discipline of Biological Sciences, The University of Newcastle, NSW 2308, Australia
    J Exp Zool A Comp Exp Biol 301:118-30. 2004
    ..However in Metatherian spermatozoa we could find no evidence that this pathway was redox regulated...
  59. doi request reprint The mouse sperm proteome characterized via IPG strip prefractionation and LC-MS/MS identification
    Mark A Baker
    The ARC Centre of Excellence in Biotechnology and Development, Reproductive Science Group, School of Environmental and Life Sciences, University of Newcastle, NSW, Australia
    Proteomics 8:1720-30. 2008
    ....
  60. ncbi request reprint Quantitative analysis of gene-specific DNA damage in human spermatozoa
    Dennis E Sawyer
    Center for Biotechnology and Development, Discipline of Biological Sciences, The University of Newcastle, University Drive, Callaghan, NSW 2308, Australia
    Mutat Res 529:21-34. 2003
    ..These studies report, for the first time, quantitative measurements of DNA damage in specific genes of male germ cells, and challenge the commonly held belief that human spermatozoa are particularly vulnerable to DNA damage...
  61. doi request reprint The rat sperm proteome characterized via IPG strip prefractionation and LC-MS/MS identification
    Mark A Baker
    The ARC Centre of Excellence in Biotechnology and Development, Reproductive Science Group, School of Environmental and Life Sciences, University of Newcastle, NSW, Australia
    Proteomics 8:2312-21. 2008
    ..This inventory of gene products, the first ever 2-D LC-MS/MS analysis of rat spermatozoa, will be invaluable in directing future research into the molecular mechanisms that drive these highly specialized cells...
  62. doi request reprint Composition and significance of detergent resistant membranes in mouse spermatozoa
    Brett Nixon
    Reproductive Science Group, Discipline of Biological Sciences, School of Environmental and Life Sciences, University of Newcastle, Callaghan, New South Wales, Australia
    J Cell Physiol 218:122-34. 2009
    ....
  63. ncbi request reprint Tyrosine phosphorylation of HSP-90 during mammalian sperm capacitation
    Heath Ecroyd
    Reproductive Science Group, School of Environmental and Life Sciences, University of Newcastle, Callaghan, New South Wales 2308, Australia
    Biol Reprod 69:1801-7. 2003
    ....
  64. pmc Gpx5 protects the family jewels
    R John Aitken
    ARC Centre of Excellence in Biotechnology and Development, Discipline of Biological Sciences, University of Newcastle, Newcastle, New South Wales, Australia
    J Clin Invest 119:1849-51. 2009
    ..These results demonstrate the key importance of Gpx5 as an extracellular antioxidant designed to protect maturing mammalian spermatozoa from oxidative stress...
  65. pmc Mobile phone radiation induces reactive oxygen species production and DNA damage in human spermatozoa in vitro
    Geoffry N De Iuliis
    ARC Centre of Excellence in Biotechnology and Development, Callaghan, New South Wales, Australia
    PLoS ONE 4:e6446. 2009
    ..In light of these associations, we have analyzed the influence of RF-EMR on the cell biology of human spermatozoa in vitro...
  66. doi request reprint The biological significance of detergent-resistant membranes in spermatozoa
    Brett Nixon
    Reproductive Science Group, Discipline of Biological Sciences, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, Australia
    J Reprod Immunol 83:8-13. 2009
    ....
  67. doi request reprint Proteomic insights into spermatozoa: critiques, comments and concerns
    Mark A Baker
    ARC Centre of Excellence in Biotechnology and Development, Discipline of Biological Sciences, School of Environmental and Life Sciences, University of Newcastle, Callaghan, New South Wales 2308, Australia
    Expert Rev Proteomics 6:691-705. 2009
    ..Three comprehensive liquid chromatography-mass spectrometry lists of human, mouse and rat spermatozoa are then compared, insights into the molecular regulation of sperm function discussed and future directions speculated upon...
  68. doi request reprint Label-free quantitation of phosphopeptide changes during rat sperm capacitation
    Mark A Baker
    The ARC Centre of Excellence in Biotechnology and Development, Priority Research Centre in Reproductive Science, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, 2308, Australia
    J Proteome Res 9:718-29. 2010
    ..This change, together with the other protein identifications reported in this study, constitute important new leads in elucidating the biochemical mechanisms by which spermatozoa attain a capacitated state...
  69. doi request reprint Investigation of the role of SRC in capacitation-associated tyrosine phosphorylation of human spermatozoa
    Lisa A Mitchell
    Reproductive Science Group, Discipline of Biological Sciences, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW 2308, Australia
    Mol Hum Reprod 14:235-43. 2008
    ..However, although the inhibition of PKA was also accompanied by a suppression of sperm motility, SRC inhibition did not induce a similar response...
  70. ncbi request reprint Identification of RARhoGAP, a novel putative RhoGAP gene expressed in male germ cells
    Benjamin J Curry
    Reproductive Science Group, Discipline of Biological Science, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW 2308, Australia
    Genomics 84:406-18. 2004
    ..We postulate that RARhoGAP may be involved in rearrangements of the cytoskeleton and cell signaling events that occur during spermatogenesis...
  71. ncbi request reprint Identification and characterization of a novel Mt-retrotransposon highly represented in the female mouse germline
    Janet E Holt
    Reproductive Science Group, Discipline of Biological Science, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW 2308, Australia
    Genomics 87:490-9. 2006
    ..Together with in silico data, we predict Mtfull plays an essential role in folliculogenesis through regulation of gene expression...
  72. ncbi request reprint The future of sex
    R John Aitken
    Hunter Medical Research Institute, the Discipline of Biological Sciences, University of Newcastle, Callaghan, New South Wales 2308, Australia
    Nature 415:963. 2002
  73. ncbi request reprint Identification of SRC as a key PKA-stimulated tyrosine kinase involved in the capacitation-associated hyperactivation of murine spermatozoa
    Mark A Baker
    The ARC Centre of Excellence in Biotechnology and Development, Reproductive Science Group, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW 2308, Australia
    J Cell Sci 119:3182-92. 2006
    ..The latter was localized to the acrosome and flagellum of murine spermatozoa by immunocytochemistry, whereas capacitation was associated with an inactivating serine phosphosphorylation of this inhibitory kinase...
  74. ncbi request reprint Identification of cytochrome-b5 reductase as the enzyme responsible for NADH-dependent lucigenin chemiluminescence in human spermatozoa
    Mark A Baker
    The ARC Centre of Excellence in Biotechnology and Development, Reproductive Science Group, School of Environmental and Life Sciences, and Hunter Medical Research Institute, University of Newcastle, Callaghan, New South Wales 2308, Australia
    Biol Reprod 73:334-42. 2005
    ..These results emphasize the importance of the direct NADH-dependent reduction of these putative superoxide-sensitive probes by cytochrome-b5 reductase even though this enzyme does not, on its own accord, produce reactive oxygen species...
  75. ncbi request reprint Localization and significance of molecular chaperones, heat shock protein 1, and tumor rejection antigen gp96 in the male reproductive tract and during capacitation and acrosome reaction
    Kelly L Asquith
    Reproductive Science Group, School of Environmental and Life Sciences, University of Newcastle, Callaghan, New South Wales 2308, Australia
    Biol Reprod 72:328-37. 2005
    ..These data reinforce the notion that these chaperones are intimately involved in the mechanisms by which mammalian spermatozoa both acquire and express their ability to recognize the zona pellucida...
  76. ncbi request reprint CXCR4/SDF1 interaction inhibits the primordial to primary follicle transition in the neonatal mouse ovary
    Janet E Holt
    Reproductive Science Group, Discipline of Biological Science, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW 2308, Australia
    Dev Biol 293:449-60. 2006
    ..By retaining follicles in an unactivated state, SDF1/CXCR4 signaling may play an important role in maintaining the size and longevity of the primordial follicle pool...
  77. ncbi request reprint Tyrosine phosphorylation activates surface chaperones facilitating sperm-zona recognition
    Kelly L Asquith
    Reproductive Science Group, School of Environmental and Life Sciences, University of Newcastle, University Drive, Callaghan, NSW 2308, Australia
    J Cell Sci 117:3645-57. 2004
    ....
  78. doi request reprint Apoptosis in the germ line
    R John Aitken
    School of Environmental and Life Sciences, Discipline of Biological Sciences and ARC Centre of Excellence in Biotechnology and Development, University of Newcastle, Callaghan, New South Wales 2308, Australia
    Reproduction 141:139-50. 2011
    ..Our ability to understand and ultimately control the reproductive potential of male and female mammals depends upon a deeper understanding of these fundamental processes...
  79. pmc Apoptosis and DNA damage in human spermatozoa
    R John Aitken
    ARC Centre of Excellence in Biotechnology and Development, Priority Research Centre in Reproductive Science, Discipline of Biological Sciences, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW 2308, Australia
    Asian J Androl 13:36-42. 2011
    ....
  80. doi request reprint The senescence-accelerated mouse prone 8 as a model for oxidative stress and impaired DNA repair in the male germ line
    T B Smith
    Reproductive Science Group, School of Environmental and Life Sciences, Priority Research Centre in Reproductive Science, Discipline of Biological Sciences, University of Newcastle, Callaghan, New South Wales 2308, Australia
    Reproduction 146:253-62. 2013
    ..001). These data highlight the role of oxidative stress and OGG1-dependent base excision repair mechanisms in defining the genetic integrity of mammalian spermatozoa. ..
  81. doi request reprint Is there a role for immunocontraception?
    E A McLaughlin
    Discipline of Biological Sciences, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW 2308, Australia
    Mol Cell Endocrinol 335:78-88. 2011
    ..We conclude that the specifications for a safe, effective, reversible vaccine are more likely to be met in animals than man...
  82. ncbi request reprint Induction of sperm maturation in vitro in epididymal cell cultures of the tammar wallaby (Macropus eugenii): disruption of motility initiation and sperm morphogenesis by inhibition of actin polymerization
    M Lin
    Cooperative Research Centre for Conservation and Management of Marsupials, Discipline of Biological Sciences, The University of Newcastle, NSW 2308, Australia
    Reproduction 124:107-17. 2002
    ..These observations also indicate that the marsupial co-culture system has the potential to greatly increase understanding of sperm-epididymal cell interactions and the mechanism of sperm maturation in these species...
  83. ncbi request reprint Biochemical entities involved in reactive oxygen species generation by human spermatozoa
    Mark A Baker
    Discipline of Biological Sciences and Hunter Medical Research Institute, University of Newcastle, Callaghan, New South Wales, Australia
    Protoplasma 221:145-51. 2003
    ..Understanding the involvement of these enzymes in sperm physiology is essential if we are to understand the causes of oxidative stress in the male germ line...
  84. doi request reprint Analysis of proteomic changes associated with sperm capacitation through the combined use of IPG-strip pre-fractionation followed by RP chromatography LC-MS/MS analysis
    Mark A Baker
    Discipline of Biological Sciences, School of Environmental and Life Sciences, University of Newcastle, NSW, Australia
    Proteomics 10:482-95. 2010
    ..To validate our data, we were able to show significant upregulation of SORD activity during capacitation, suggesting that the polyol pathway is activated during this process...
  85. doi request reprint Phosphorylation and consequent stimulation of the tyrosine kinase c-Abl by PKA in mouse spermatozoa; its implications during capacitation
    Mark A Baker
    ARC Centre of Excellence in Biotechnology and Development, Reproductive Science Group, School of Environmental and Life Sciences, University of Newcastle, NSW 2308, Australia
    Dev Biol 333:57-66. 2009
    ..We present a model, whereby these kinases may operate together with SRC to ensure optimal levels of tyrosine phosphorylation in the sperm flagellum during the attainment of a capacitated state...
  86. doi request reprint DNA damage in human spermatozoa is highly correlated with the efficiency of chromatin remodeling and the formation of 8-hydroxy-2'-deoxyguanosine, a marker of oxidative stress
    Geoffry N De Iuliis
    ARC Centre of Excellence in Biotechnology and Development and Hunter Medical Research Institute, Discipline of Biological Sciences, University of Newcastle, Callaghan, New South Wales 2308, Australia
    Biol Reprod 81:517-24. 2009
    ..These results clearly highlight the importance of oxidative stress in the induction of sperm DNA damage and carry significant implications for the clinical management of this condition...
  87. doi request reprint Significance of mitochondrial reactive oxygen species in the generation of oxidative stress in spermatozoa
    Adam J Koppers
    Discipline of Biological Sciences, University of Newcastle Callaghan, New South Wales 2308, Australia
    J Clin Endocrinol Metab 93:3199-207. 2008
    ..Male infertility has been linked with the excessive generation of reactive oxygen species (ROS) by defective spermatozoa. However, the subcellular origins of this activity are unclear...
  88. doi request reprint Prolactin exerts a prosurvival effect on human spermatozoa via mechanisms that involve the stimulation of Akt phosphorylation and suppression of caspase activation and capacitation
    Dwi Ari Pujianto
    School of Environmental and Life Sciences, University of Newcastle, University Drive Callaghan, New South Wales 2308, Australia
    Endocrinology 151:1269-79. 2010
    ..These findings have implications for preservation of sperm integrity in vivo and in vitro...
  89. doi request reprint The spermostatic and microbicidal actions of quinones and maleimides: toward a dual-purpose contraceptive agent
    Louise M Hughes
    School of Environmental and Life Sciences, Faculty of Science and IT, University of Newcastle, Callaghan, NSW, Australia
    Mol Pharmacol 76:113-24. 2009
    ..Taken together, these results have identified for the first time a novel set of cellular targets and chemical principles capable of providing simultaneous defense against both fertility and the spread of sexually transmitted disease...
  90. doi request reprint The source and significance of DNA damage in human spermatozoa; a commentary on diagnostic strategies and straw man fallacies
    R J Aitken
    Discipline of Biological Sciences and Priority Research Centre in Reproductive Science, Faculty of Science and IT, University of Newcastle, University Drive, Callaghan, NSW 2308, Australia
    Mol Hum Reprod 19:475-85. 2013
    ....
  91. doi request reprint Investigation of the expression and functional significance of the novel mouse sperm protein, a disintegrin and metalloprotease with thrombospondin type 1 motifs number 10 (ADAMTS10)
    M D Dun
    Discipline of Biological Sciences, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, Australia
    Int J Androl 35:572-89. 2012
    ..Overall, these studies support the notion that sperm-oocyte interactions involve considerable functional redundancy and identify ADAMTS10 as a novel candidate in the mediation of these fundamentally important events...
  92. ncbi request reprint A comparative study of oxidative DNA damage in mammalian spermatozoa
    Liga E Bennetts
    Discipline of Biological Sciences, The University of Newcastle, New South Wales, Australia
    Mol Reprod Dev 71:77-87. 2005
    ....
  93. ncbi request reprint Identification and characterization of a novel splice variant of mouse and rat cytochrome b5/cytochrome b5 reductase
    Benjamin J Curry
    ARC Centre of Excellence for Biotechnology and Development, Discipline of Biological Sciences, School of Environmental and Life Sciences, University of Newcastle, University Drive, Callaghan, NSW 2308, Australia
    Genomics 83:425-38. 2004
    ..We postulate that cb5/cb5rdelta12 may function in a dominant negative fashion, limiting the amount of damage caused by the production of reactive oxygen species by cb5/cb5r...
  94. doi request reprint Unexplained antepartum stillbirth: a consequence of placental aging?
    R Smith
    Hunter Medical Research Institute, University of Newcastle, Lookout Road, Newcastle, NSW 2310, Australia
    Placenta 34:310-3. 2013
    ..Premature aging may also play an important role in antepartum stillbirth occurring earlier in pregnancy, especially in the context of growth restriction...
  95. ncbi request reprint Detection of Ehrlichia platys in dogs in Australia
    G K Brown
    Discipline of Biological Sciences, School of Biological and Chemical Sciences, University of Newcastle, Callaghan, New South Wales
    Aust Vet J 79:554-8. 2001
    ..By using universal primers directed against bacterial 16S ribosomal DNA and sequencing analysis, the detection of potentially pathogenic Ehrlichia organisms that had not previously been found in Australia has been made possible...
  96. ncbi request reprint Vitamin A regulation of BMP4 expression in the male germ line
    Rosa M Baleato
    Reproductive Science Group, School of Environmental and Life Sciences, University of Newcastle, NSW 2308, Australia
    Dev Biol 286:78-90. 2005
    ..Our results reflect a direct requirement for retinoids by germ cells for the resumption of spermatogenesis in VAD animals via mechanisms that involve the suppression of BMP4 expression...
  97. ncbi request reprint The contraceptive potential of ZP3 and ZP3 peptides in a primate model
    Margaret Paterson
    MRC Human Reproductive Sciences Unit, Centre for Reproductive Biology, 37, Chalmers Street, Edinburgh, EH3 9ET, UK
    J Reprod Immunol 53:99-107. 2002
    ..Thus considerable research is still required to identify a combination of ZP epitopes that will induce reversible infertility in the absence of any ovarian dysfunction...
  98. ncbi request reprint Male reproductive health and the environment
    R John Aitken
    Med J Aust 185:414-5. 2006
  99. ncbi request reprint Integrating new tests of sperm genetic integrity into semen analysis: breakout group discussion
    Sally D Perreault
    U S EPA, ORD, NHEERL, Reproductive Toxicology Division, Research Triangle Park, NC 27711, USA
    Adv Exp Med Biol 518:253-68. 2003
  100. ncbi request reprint Cysteine-rich secretory protein 2 binds to mitogen-activated protein kinase kinase kinase 11 in mouse sperm
    Gerard M Gibbs
    Monash Institute of Medical Research, Monash University, Melbourne, Victoria 3168, Australia
    Biol Reprod 77:108-14. 2007
    ..These data raise the possibility that CRISP2 is a MAP3K11-modifying protein or, alternatively, that MAP3K11 acts to phosphorylate CRISP2 during acrosome development...
  101. ncbi request reprint Polyunsaturated fatty acids in male and female reproduction
    D Claire Wathes
    Department of Veterinary Basic Sciences, Royal Veterinary College, North Mymms, Hatfield, Herts, AL9 7TA, United Kingdom
    Biol Reprod 77:190-201. 2007
    ..Adequately powered trials that control for the ratios of different PUFAs consumed are required to determine the extent to which this aspect of our diets does influence our fertility...