Research Topics
Genomes and Genes
| Donald MetcalfSummaryAffiliation: The Walter and Eliza Hall Institute of Medical Research Country: Australia Publications
| Collaborators
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Detail Information
Publications
The lethal effects of transplantation of Socs1-/- bone marrow cells into irradiated adult syngeneic recipientsDonald Metcalf
Division of Cancer and Hematology, The Walter and Eliza Hall Institute of Medical Research and the Cooperative Research Centre for Cellular Growth Factors, 1G Royal Parade, Parkville, Victoria 3050, Australia
Proc Natl Acad Sci U S A 100:8436-41. 2003....- Crowding-dependent production of colony-stimulating factors by cultured syngeneic or allogeneic hematopoietic cellsDonald Metcalf
Division of Cancer and Hematology, The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, 3050 Victoria, Australia
Proc Natl Acad Sci U S A 100:1244-9. 2003.... 
The unsolved enigmas of leukemia inhibitory factorDonald Metcalf
Division of Cancer and Haematology, The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia
Stem Cells 21:5-14. 2003....- Murine megakaryocyte progenitor cells and their susceptibility to suppression by G-CSFDonald Metcalf
Division of Cancer and Hematology, The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria 3050, Australia
Stem Cells 23:55-62. 2005.... - Synergistic and inhibitory interactions in the in vitro control of murine megakaryocyte colony formationDonald Metcalf
Division of Cancer and Hematology, The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia
Stem Cells 20:552-60. 2002..The mechanisms involved in this inhibitory action of G-CSF are unknown, but the inhibitory action could be of relevance for the dose-dependent lowering of platelet levels observed in some subjects injected with G-CSF... - Loss of PU.1: one of the many roads to myeloid leukemiaDonald Metcalf
The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3050, Australia
Cell Cycle 5:673-4. 2006 - Stem cell factor can stimulate the formation of eosinophils by two types of murine eosinophil progenitor cellsDonald Metcalf
Division of Cancer and Hematology, The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia
Stem Cells 20:460-9. 2002..The observed activity of SCF in marrow cultures was relatively weak, but its action may be stronger in vivo, and SCF needs to be added to GM-CSF, IL-5, and IL-3 in the list of cytokines able to stimulate eosinophil formation... - On hematopoietic stem cell fateDonald Metcalf
The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville 3050, Victoria, Australia
Immunity 26:669-73. 2007..Here, Metcalf considers the origin and heterogeneity of HSCs, their ability to self-generate, and their commitment to the various hematopoietic lineages... - Concise review: hematopoietic stem cells and tissue stem cells: current concepts and unanswered questionsDonald Metcalf
The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria 3050, Australia
Stem Cells 25:2390-5. 2007..Disclosure of potential conflicts of interest is found at the end of this article... - Clonogenic mast cell progenitors and their excess numbers in chimeric BALB/c mice with inactivated GATA-1Donald Metcalf
The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville 3050, Victoria, Australia
Proc Natl Acad Sci U S A 104:18642-7. 2007..No excess tissue mast cells were observed in GATA-1(Plt13/+) mice, suggesting that the excess mast cell progenitors in these mice might be generating mast cells with a defective in vivo proliferative or tissue homing capacity... - The colony-stimulating factors and cancerDonald Metcalf
The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria 3052, Australia
Nat Rev Cancer 10:425-34. 2010.... - Hematopoietic cytokinesDonald Metcalf
Walter and Eliza Hall Institute of Medical Research, Victoria, Australia
Blood 111:485-91. 2008.... - Polycystic kidneys and chronic inflammatory lesions are the delayed consequences of loss of the suppressor of cytokine signaling-1 (SOCS-1)Donald Metcalf
The Walter and Eliza Hall Institute of Medical Research, and The Cooperative Research Centre for Cellular Growth Factors, Post Office Royal Melbourne Hospital, Victoria 3050, Australia
Proc Natl Acad Sci U S A 99:943-8. 2002..The chronic infections and granulomas of SOCS-1(-/-) IFN-gamma(-/-) mice may be based on autoaggression of SOCS-1(-/-) T lymphoid and related cells or a functional deficiency of these cells when lacking SOCS-1... - Murine hematopoietic blast colony-forming cells and their progeny have distinctive membrane marker profilesDonald Metcalf
The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria 3052, Australia
Proc Natl Acad Sci U S A 106:19102-7. 2009..The commitment events and phenotypic changes during the generation of differing progenitor cells in blast colonies can now be clonally analyzed in a convenient in vitro culture system... - Lineage commitment and maturation induction in normal and leukemic preprogenitor cellsD Metcalf
Walter and Eliza Hall Institute of Medical Research, P O Royal Melbourne Hospital, 3050 Victoria, Australia
Ann N Y Acad Sci 938:278-91; discussion 291-2. 2001.... - Production of colony-stimulating factors and IL-5 by organs from three types of mice with inflammatory disease due to loss of the suppressor of cytokine signaling-1D Metcalf
Walter and Eliza Hall Institute of Medical Research and the Cooperative Research Centre for Cellular Growth Factors, Royal Melbourne Hospital, Victoria, Australia
J Immunol 167:4661-7. 2001..The ability of local organs to produce these proinflammatory cytokines could contribute to the development and progression of these inflammatory lesions... - Multipotential hematopoietic blast colony-forming cells exhibit delays in self-generation and lineage commitmentDonald Metcalf
Walter and Eliza Hall Institute of Medical Research, Parkville 3052, Australia
Proc Natl Acad Sci U S A 107:16257-61. 2010..Because BL-CFCs can self-generate and are able to generate lymphoid and myeloid populations, BL-CFCs appear to be ideal cells in which to analyze the processes of self-generation and lineage commitment in clonal in vitro cultures... - The preleukemic state of mice reconstituted with Mixl1-transduced marrow cellsDonald Metcalf
The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria 3050, Australia
Proc Natl Acad Sci U S A 104:20013-8. 2007.... - Inactivation of PU.1 in adult mice leads to the development of myeloid leukemiaDonald Metcalf
The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville 3050, Victoria, Australia
Proc Natl Acad Sci U S A 103:1486-91. 2006..Acquisition of autocrine growth-factor production and autonomous growth appeared to be major events in the transformation of conditionally deleted PU.1(-/-) cells to fully developed myeloid leukemic populations... 
Mcl-1 and Bcl-x(L) coordinately regulate megakaryocyte survivalMarlyse A Debrincat
Cancer and Hematology Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, Australia
Blood 119:5850-8. 2012..Our studies indicate that the combination of Bcl-x(L) and Mcl-1 is essential for the viability of the megakaryocyte lineage...- PU.1 regulates the commitment of adult hematopoietic progenitors and restricts granulopoiesisAleksandar Dakic
The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3050, Australia
J Exp Med 201:1487-502. 2005..These findings emphasize the distinct nature of adult hematopoiesis and reveal that PU.1 regulates the specification of the multipotent lymphoid and myeloid compartments and restrains, rather than promotes, granulopoiesis... - A family of cytokine-inducible inhibitors of signallingR Starr
The Walter and Eliza Hall Institute for Medical Research, Parkville, Victoria, Australia
Nature 387:917-21. 1997..Transcription of all four SOCS genes is increased rapidly in response to interleukin-6, in vitro and in vivo, suggesting they may act in a classic negative feedback loop to regulate cytokine signal transduction... - Suppressor of cytokine signaling-1 has IFN-gamma-independent actions in T cell homeostasisAnn L Cornish
The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia
J Immunol 170:878-86. 2003..Thus, SOCS-1 is a critical regulator of T cell activation and homeostasis, and its influence extends beyond regulating IFN-gamma signaling... - Erg is required for self-renewal of hematopoietic stem cells during stress hematopoiesis in miceAshley P Ng
Walter and Eliza Hall Institute of Medical Research, Parkville, Australia
Blood 118:2454-61. 2011..Thus, Erg is a critical regulator of adult HSCs, essential for maintaining self-renewal at times of high HSC cycling... - Socs3 maintains the specificity of biological responses to cytokine signals during granulocyte and macrophage differentiationBen A Croker
Cancer and Haematology Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia
Exp Hematol 36:786-98. 2008..The tight regulation by Socs3 of signal transducer and activator of transcription 3 phosphorylation and gene transcription after cytokine receptor ligation significantly influences the fate of myeloid progenitor cells... - The SOCS box of suppressor of cytokine signaling-3 contributes to the control of G-CSF responsiveness in vivoKristy Boyle
The Walter and Eliza Hall Institute of Medical Research, Parkville, Australia
Blood 110:1466-74. 2007..Overall, the results establish a role for the SOCS box of SOCS3 in the in vivo regulation of G-CSF signaling and the response to inflammatory stimuli... - SOCS3 negatively regulates IL-6 signaling in vivoBen A Croker
Cancer and Haematology Division, The Walter and Eliza Hall Institute of Medical Research and the Cooperative Research Centre for Cellular Growth Factors, 1G Royal Parade, Parkville, Victoria 3050, Australia
Nat Immunol 4:540-5. 2003..Our data indicate that SOCS3 and SOCS1 have reciprocal functions in IL-6 and IFN-gamma regulation and imply that SOCS3 has a role in preventing IFN-gamma-like responses in cells stimulated by IL-6... - Regulation of hematopoietic stem cells by their mature progenyCarolyn A de Graaf
Molecular Medicine Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3052, Australia
Proc Natl Acad Sci U S A 107:21689-94. 2010..These studies suggest that disorders of platelet number can have profound effects on the HSC compartment via effects on the feedback regulation of circulating TPO concentration... - SOCS3 is a critical physiological negative regulator of G-CSF signaling and emergency granulopoiesisBen A Croker
Cancer and Haematology Division, The Walter and Eliza Hall Institute of Medical Research and the Cooperative Research Centre for Cellular Growth Factors, Parkville, Victoria 3050, Australia
Immunity 20:153-65. 2004..We conclude that SOCS3 is a key negative regulator of G-CSF signaling in myeloid cells and that this is of particular significance during G-CSF-driven emergency granulopoiesis... - Hematopoietic overexpression of the transcription factor Erg induces lymphoid and erythro-megakaryocytic leukemiaCatherine L Carmichael
Cancer and Hematology Division, The Walter and Eliza Hall Institute of Medical Research, Parkville 3052, Vic, Australia
Proc Natl Acad Sci U S A 109:15437-42. 2012..Thus, in mice, Erg overexpression induces the development of not only lymphoid leukemia but also erythro-megakaryocytic leukemia... - IL-6 promotes acute and chronic inflammatory disease in the absence of SOCS3Ben A Croker
Department of Inflammation, The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia
Immunol Cell Biol 90:124-9. 2012..We conclude that SOCS3 is required for survival during inflammatory responses and is a critical regulator of IL-6 in vivo... - Fas-mediated neutrophil apoptosis is accelerated by Bid, Bak, and Bax and inhibited by Bcl-2 and Mcl-1Ben A Croker
The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3052, Australia
Proc Natl Acad Sci U S A 108:13135-40. 2011..These data demonstrate that the intrinsic apoptosis pathway directly controls the kinetics of Fas-triggered apoptosis in neutrophils... - Dual requirement for the ETS transcription factors Fli-1 and Erg in hematopoietic stem cells and the megakaryocyte lineageElizabeth A Kruse
Divisions of Molecular Medicine and Cancer and Haematology, The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville 3052, Australia
Proc Natl Acad Sci U S A 106:13814-9. 2009..These results illustrate an indispensable requirement for both Fli-1 and Erg in normal HSC and megakaryocyte homeostasis, and suggest these transcription factors may coregulate common target genes... - Trisomy of Erg is required for myeloproliferation in a mouse model of Down syndromeAshley P Ng
Cancer and Haematology Division, The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia
Blood 115:3966-9. 2010.... - Point mutation in the gene encoding p300 suppresses thrombocytopenia in Mpl-/- miceMaria Kauppi
Cancer and Haematology, The Walter and Eliza Hall Institute of Medical Research, Parkville, Australia
Blood 112:3148-53. 2008.... - Elevated Mcl-1 perturbs lymphopoiesis, promotes transformation of hematopoietic stem/progenitor cells, and enhances drug resistanceKirsteen J Campbell
Molecular Genetics of CancerDivision, Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville 3052, Victoria, Australia
Blood 116:3197-207. 2010..Collectively, these results demonstrate that Mcl-1 overexpression renders hematopoietic cells refractory to many cytotoxic insults, perturbs lymphopoiesis and promotes malignant transformation of hematopoietic stem and progenitor cells... - Anomalous megakaryocytopoiesis in mice with mutations in the c-Myb geneDonald Metcalf
Division of Cancer and Hematology, The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria 3050, Australia
Blood 105:3480-7. 2005.... - Reduced lymphocyte longevity and homeostatic proliferation in lamin B receptor-deficient mice results in profound and progressive lymphopeniaAnne M Verhagen
Walter and Eliza Hall Institute, Parkville, Victoria 3052, Australia
J Immunol 188:122-34. 2012..In summary, this study demonstrates that LBR deficiency affects not only nuclear architecture but also proliferation, cell viability, and gene expression of hematopoietic cells... - Blimp1 is limiting for transformation in a mouse plasmacytoma modelKathy D'Costa
Division of Immunology, The Walter and Eliza Hall Institute of Medical Research, Parkville, Australia
Blood 113:5911-9. 2009..These observations suggest that Blimp1 is limiting for plasma cell transformation and thus has potential as a target for new therapies to combat MM... - SOCS2 negatively regulates growth hormone action in vitro and in vivoChristopher J Greenhalgh
Cancer and Haematology Division, The Walter and Eliza Hall Institute of Medical Research WEHI and the Cooperative Centre for Cellular Growth Factors, Parkville, Victoria, Australia
J Clin Invest 115:397-406. 2005..Together, the data provide clear evidence that SOCS2 is a negative regulator of GH signaling... - The transcription factor Erg is essential for definitive hematopoiesis and the function of adult hematopoietic stem cellsStephen J Loughran
Division of Molecular Medicine, The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3050, Australia
Nat Immunol 9:810-9. 2008..We show that Erg is required for definitive hematopoiesis, adult hematopoietic stem cell function and the maintenance of normal peripheral blood platelet numbers... - Enforced expression of the homeobox gene Mixl1 impairs hematopoietic differentiation and results in acute myeloid leukemiaStefan Glaser
The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria 3050, Australia
Proc Natl Acad Sci U S A 103:16460-5. 2006..These observations establish a link between enforced Mixl1 expression and leukemogenesis in the mouse... - Oct2 enhances antibody-secreting cell differentiation through regulation of IL-5 receptor alpha chain expression on activated B cellsDianne Emslie
Immunology Division, The Walter and Eliza Hall Institute, Parkville, Victoria 3050, Australia
J Exp Med 205:409-21. 2008..IL-5 augments ASC differentiation in vitro, and we show that IL-5 directly activates the plasma cell differentiation program by enhancing blimp1 expression... - A mutation in the translation initiation codon of Gata-1 disrupts megakaryocyte maturation and causes thrombocytopeniaIan J Majewski
The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria 3050, Australia
Proc Natl Acad Sci U S A 103:14146-51. 2006..Although the equivalent mutation to Gata-1Plt13 in humans results in production of GATA-1s, a short protein isoform initiated from a start codon downstream of the mutated initiation codon, Gata-1s was not detected in Gata-1Plt13/+ mice... - Monocytic leukemia zinc finger protein is essential for the development of long-term reconstituting hematopoietic stem cellsTim Thomas
The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3050, Australia
Genes Dev 20:1175-86. 2006.... - Programmed anuclear cell death delimits platelet life spanKylie D Mason
Molecular Genetics of Cancer Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3050, Australia
Cell 128:1173-86. 2007.... - The critical regulator of embryonic hematopoiesis, SCL, is vital in the adult for megakaryopoiesis, erythropoiesis, and lineage choice in CFU-S12Mark A Hall
Rotary Bone Marrow Research Laboratory, Royal Melbourne Hospital, Melbourne, Victoria 3050, Australia
Proc Natl Acad Sci U S A 100:992-7. 2003..These results suggest that SCL is critical for megakaryopoiesis and erythropoiesis, but is dispensable for production of myeloid cells during adult hematopoiesis... - Characterization of thrombopoietin (TPO)-responsive progenitor cells in adult mouse bone marrow with in vivo megakaryocyte and erythroid potentialAshley P Ng
The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3052, Australia
Proc Natl Acad Sci U S A 109:2364-9. 2012.... - Thrombocytopenia and erythrocytosis in mice with a mutation in the gene encoding the hemoglobin β minor chainMaria Kauppi
Cancer and Haematology Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, 3052, Australia
Proc Natl Acad Sci U S A 109:576-81. 2012..The Plt12 mouse is a model of high O(2)-affinity hemoglobinopathy and provides insights into hematopoiesis under conditions of chronic hypoxia... - Hematopoietic defects in the Ts1Cje mouse model of Down syndromeCatherine L Carmichael
Molecular Medicine, Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia
Blood 113:1929-37. 2009..Thus, loss of Gata1 and partial trisomy of chromosome 21 orthologs, when combined, do not appear to be sufficient to induce TMD or AMKL-like phenotypes in mice... - A key role for G-CSF-induced neutrophil production and trafficking during inflammatory arthritisJo L Eyles
The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia
Blood 112:5193-201. 2008.... - Architectural defects in the spleens of Nkx2-3-deficient mice are intrinsic and associated with defects in both B cell maturation and T cell-dependent immune responsesDavid Tarlinton
Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia
J Immunol 170:4002-10. 2003..Collectively, these data indicate a substantial role for Nkx2-3 in the correct association of lymphocytes and splenic stromal elements that is independent of chemokine expression... - Transcriptional repressor Blimp-1 is essential for T cell homeostasis and self-toleranceAxel Kallies
The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3050, Australia
Nat Immunol 7:466-74. 2006..These data identify Blimp-1 as an essential regulator of T cell homeostasis and suggest that Blimp-1 regulates both B cell and T cell differentiation... - Genetic influences determining progenitor cell mobilization and leukocytosis induced by granulocyte colony-stimulating factorA W Roberts
The Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Victoria, Australia
Blood 89:2736-44. 1997..5), and that this approach may serve as a useful model for the identification of genes involved in the mobilization process... - A kinase-dead allele of Lyn attenuates autoimmune disease normally associated with Lyn deficiencyAnne M Verhagen
Signal Transduction Laboratory, St Vincent s Institute, Fitzroy, Victoria, Australia
J Immunol 182:2020-9. 2009..Furthermore, our results provide evidence for an additional role for Lyn kinase, distinct from its catalytic activity, in regulating intracellular signaling pathways... - Polycomb repressive complex 2 (PRC2) restricts hematopoietic stem cell activityIan J Majewski
The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia
PLoS Biol 6:e93. 2008..These data suggest that PRC2 is required to maintain a specific gene expression pattern in hematopoiesis that is indispensable to normal stem cell function... - The absence of Tssc6, a member of the tetraspanin superfamily, does not affect lymphoid development but enhances in vitro T-cell proliferative responsesJacqueline M Tarrant
The Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, 3050 Victoria, Australia
Mol Cell Biol 22:5006-18. 2002..These results demonstrate that Tssc6 is not required for normal development of the hematopoietic system but may play a role in the negative regulation of peripheral T-lymphocyte proliferation... - Suppressor screen in Mpl-/- mice: c-Myb mutation causes supraphysiological production of platelets in the absence of thrombopoietin signalingMarina R Carpinelli
The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville VIC 3052, Australia
Proc Natl Acad Sci U S A 101:6553-8. 2004.... - Agm1/Pgm3-mediated sugar nucleotide synthesis is essential for hematopoiesis and developmentKylie T Greig
Division of Molecular Medicine, The Walter and Eliza Hall Institute of Medical Research, Victoria, Australia
Mol Cell Biol 27:5849-59. 2007.... - Genetic reduction of embryonic leukemia-inhibitory factor production rescues placentation in SOCS3-null embryos but does not prevent inflammatory diseaseLorraine Robb
The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria 3050, Australia
Proc Natl Acad Sci U S A 102:16333-8. 2005.... - Megakaryocytes possess a functional intrinsic apoptosis pathway that must be restrained to survive and produce plateletsEmma C Josefsson
Molecular Medicine Division, Cancer and Hematology Division, The Walter and Eliza Hall Institute of Medical Research, Parkville 3052, Australia Department of Medical Biology, The University of Melbourne, Parkville 3010, Australia
J Exp Med 208:2017-31. 2011..Thus, megakaryocytes do not activate the intrinsic pathway to generate platelets; rather, the opposite is true: they must restrain it to survive and progress safely through proplatelet formation and platelet shedding... - Development of plasmacytoid and conventional dendritic cell subtypes from single precursor cells derived in vitro and in vivoShalin H Naik
The Walter and Eliza Hall Institute, Parkville, Victoria 3050, Australia
Nat Immunol 8:1217-26. 2007..Thus, commitment to particular DC subtypes begins mainly at this pro-DC stage... - Development of hydrocephalus in mice lacking SOCS7Danielle L Krebs
The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria 3050, Australia
Proc Natl Acad Sci U S A 101:15446-51. 2004..In situ hybridization studies revealed prominent expression of Socs7 in the brain, suggestive of an important functional role of SOCS7 in this organ... - Thrombocytopenia and kidney disease in mice with a mutation in the C1galt1 geneWarren S Alexander
The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria 3050, Australia
Proc Natl Acad Sci U S A 103:16442-7. 2006..The disease in plt1/plt1 mice suggests that, in addition to immune-mediated effects, intrinsic C1Gal-T1 deficiency in megakaryocytes and the kidney may contribute to pathology... - SOCS5 is expressed in primary B and T lymphoid cells but is dispensable for lymphocyte production and functionChristine Brender
The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3050, Australia
Mol Cell Biol 24:6094-103. 2004..Therefore, although SOCS5 is expressed in primary B and T cells, it appears to be dispensable for the regulation of lymphocyte function... - FADD and caspase-8 are required for cytokine-induced proliferation of hemopoietic progenitor cellsMarc Pellegrini
The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville VIC 3050, Australia
Blood 106:1581-9. 2005..These data demonstrate that the enzymatic activity of caspase-8 and its adaptor FADD are required for cytokine-induced proliferation of hemopoietic progenitor cells... - The conserved SOCS box motif in suppressors of cytokine signaling binds to elongins B and C and may couple bound proteins to proteasomal degradationJ G Zhang
Walter and Eliza Hall Institute of Medical Research and the Cooperative Research Centre for Cellular Growth Factors, P O Royal Melbourne Hospital, Victoria 3050, Australia
Proc Natl Acad Sci U S A 96:2071-6. 1999..Analogous to the family of F-box-containing proteins, it appears that the SOCS proteins may act as adaptor molecules that target activated cell signaling proteins to the protein degradation pathway... - Intrasplenic steady-state dendritic cell precursors that are distinct from monocytesShalin H Naik
The Walter and Eliza Hall Institute of Medical Research and the Cooperative Research Centre for Vaccine Technology, Parkville, Victoria 3050, Australia
Nat Immunol 7:663-71. 2006..Thus, the inflammatory status of the host influences the developmental origin and type of DC present in lymphoid tissues... - Dynamic regulation of PU.1 expression in multipotent hematopoietic progenitorsStephen L Nutt
The Walter and Eliza Hall Institute of Medical Research, Victoria 3050, Australia
J Exp Med 201:221-31. 2005..1(lo) CMPs were the immediate precursors of the MEP. Thus, down-regulation of PU.1 expression in the CMP is the first molecularly identified event associated with the restriction of differentiation to erythroid and megakaryocyte lineages... - Murine flt3 ligand protects M1 leukemic cells from LIF-induced differentiation and suppression of self-renewalC G Begley
Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Victoria, Australia
Exp Hematol 24:1247-57. 1996.... - Liver degeneration and lymphoid deficiencies in mice lacking suppressor of cytokine signaling-1R Starr
Walter and Eliza Hall Institute of Medical Research and Cooperative Research Centre for Cellular Growth Factors, Post Office Royal Melbourne Hospital, Victoria 3050, Australia
Proc Natl Acad Sci U S A 95:14395-9. 1998..Thus, SOCS-1 is required for in vivo regulation of multiple cell types and is indispensable for normal postnatal growth and survival... - Suppressor of cytokine signaling (SOCS)-5 is a potential negative regulator of epidermal growth factor signalingSandra E Nicholson
The Walter and Eliza Hall Institute of Medical Research, Cooperative Research Centre for Cellular Growth Factors, Parkville, Victoria 3052, Australia
Proc Natl Acad Sci U S A 102:2328-33. 2005..This result suggests that the inhibition of signaling occurs through enhanced proteasomal degradation of the EGF-R through SOCS box recruitment of E3 ubiquitin ligase activity... - A novel mutation in the Nfkb2 gene generates an NF-kappa B2 "super repressor"Elena Tucker
St Vincent s Institute, Fitzroy, Victoria, Australia
J Immunol 179:7514-22. 2007..These findings demonstrate a key role for NF-kappaB2 in the regulation of RelA activation and suggest overlap in the function of NF-kappaB members in canonical and noncanonical pathway signaling... - The residual megakaryocyte and platelet production in c-mpl-deficient mice is not dependent on the actions of interleukin-6, interleukin-11, or leukemia inhibitory factorT Gainsford
The Walter and Eliza Hall Institute for Medical Research, the Cooperative Research Centre for Cellular Growth Factors and the Rotary Bone Marrow Research Laboratories, Royal Melbourne Hospital, Victoria, Australia
Blood 95:528-34. 2000..Blood. 2000;95:528-534).. - Deficiencies in progenitor cells of multiple hematopoietic lineages and defective megakaryocytopoiesis in mice lacking the thrombopoietic receptor c-MplW S Alexander
The Walter and Eliza Hall Institute for Medical Research, Royal Melbourne Hospital, Victoria, Australia
Blood 87:2162-70. 1996..Moreover, our results also imply an important role for TPO and c-Mpl in the production of primitive pluripotent progenitor cells as well as progenitor cells committed to nonmegakaryocytic lineages... - Constitutive Bcl-2 expression throughout the hematopoietic compartment affects multiple lineages and enhances progenitor cell survivalS Ogilvy
The Walter and Eliza Hall Institute of Medical Research, Post Office, Royal Melbourne Hospital, Victoria 3050, Australia
Proc Natl Acad Sci U S A 96:14943-8. 1999..Thus, Bcl-2 has multiple effects on the hematopoietic system. These mice should help to further clarify the role of apoptosis in the development and homeostasis of this compartment... - Mice lacking the c-rel proto-oncogene exhibit defects in lymphocyte proliferation, humoral immunity, and interleukin-2 expressionF Kontgen
Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Victoria, Australia
Genes Dev 9:1965-77. 1995..The ability of exogenous interleukin-2 to restore T Cell, but not B cell, proliferation indicates that Rel regulates the expression of different genes in B and T cells that are crucial for cell division and immune function... - Hematopoietic and lung abnormalities in mice with a null mutation of the common beta subunit of the receptors for granulocyte-macrophage colony-stimulating factor and interleukins 3 and 5L Robb
Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Victoria, Australia
Proc Natl Acad Sci U S A 92:9565-9. 1995..The data establish that beta C is normally limiting for high-affinity binding of GM-CSF and demonstrate that systemic clearance of GM-CSF is not mediated via such high-affinity receptor complexes... - Development of dendritic cells in culture from human and murine thymic precursor cellsK A Kelly
The Walter and Eliza Hall Institute of Medical Research, Post Office Royal Melbourne Hospital, Victoria, Australia
Cell Mol Biol (Noisy-le-grand) 47:43-54. 2001..Thus, although apparently lymphoid-restricted, the human thymic DC precursors responded to the myeloid factor GM-CSF as well as to the cytokines selective for murine lymphoid-related DC... - Proapoptotic Bcl-2 relative Bim required for certain apoptotic responses, leukocyte homeostasis, and to preclude autoimmunityP Bouillet
The Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria 3050, Australia
Science 286:1735-8. 1999..Thus, Bim is required for hematopoietic homeostasis and as a barrier to autoimmunity. Moreover, particular death stimuli appear to activate apoptosis through distinct BH3-only proteins... - Two distinct types of murine blast colony-forming cells are multipotential hematopoietic precursorsD Metcalf
The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria 3050, Australia
Proc Natl Acad Sci U S A 105:18501-6. 2008..Both types of blast colonies offer remarkable opportunities to analyze multilineage commitment at a clonal level in vitro... - SOCS1 is a critical inhibitor of interferon gamma signaling and prevents the potentially fatal neonatal actions of this cytokineW S Alexander
The Walter and Eliza Hall Institute of Medical Research, Victoria, Australia
Cell 98:597-608. 1999..Our data indicate that SOCS1 is a key modulator of IFNgamma action, allowing the protective effects of this cytokine to occur without the risk of associated pathological responses... - Aberrant hematopoiesis in mice with inactivation of the gene encoding SOCS-1D Metcalf
The Walter and Eliza Hall Institute of Medical Research, PO Royal Melbourne Hospital, Victoria, Australia
Leukemia 13:926-34. 1999..The suppressive effects of IFN-gamma therefore appear to involve interactions with particular growth factor-initiated signals in -/- cells--interactions that are strongly modulated by the action of the SOCS-1 protein... - Critical role for granulocyte colony-stimulating factor in inflammatory arthritisKate E Lawlor
The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3050, Australia
Proc Natl Acad Sci U S A 101:11398-403. 2004..Our results reveal a critical role for G-CSF in driving joint inflammation and highlight G-CSF as a potential therapeutic target in inflammatory joint diseases, such as rheumatoid arthritis... - Gigantism in mice lacking suppressor of cytokine signalling-2D Metcalf
The Walter and Eliza Hall Institute of Medical Research and the Cooperative Research Centre for Cellular Growth Factors, Royal Melbourne Hospital, Victoria, Australia
Nature 405:1069-73. 2000.... - The SOCS-1 storyD Metcalf
The Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Victoria, Australia
Exp Hematol 27:1715-23. 1999..SOCS-1-/- mice allowed to survive the neonatal period by cross-mating with IFN-gamma-/- mice may well ultimately develop other disease states, because loss of SOCS-1 potentially renders them hyperresponsive to other cytokine signaling... - The unusual species cross-reactivity of the leukemia inhibitory factor receptor alpha-chain is determined primarily by the immunoglobulin-like domainC M Owczarek
Walter and Eliza Hall Institute of Medical Research and the Cooperative Research Centre for Cellular Growth Factors, P O Royal Melbourne Hospital, Victoria 3050, Australia
J Biol Chem 272:23976-85. 1997..These findings should enable the development of a "humanized" mouse LIFR that could act as a potent antagonist of hLIF biological activities in vivo... - The SOCS box of suppressor of cytokine signaling-1 is important for inhibition of cytokine action in vivoJ G Zhang
The Walter and Eliza Hall Institute of Medical Research and the Cooperative Research Centre for Cellular Growth Factors, P O Royal Melbourne Hospital, Parkville, VIC 3050, Australia
Proc Natl Acad Sci U S A 98:13261-5. 2001..These results demonstrate that deletion of the SOCS box leads to a partial loss of function of SOCS-1... - Cellular processing of murine colony-stimulating factor (Multi-CSF, GM-CSF, G-CSF) receptors by normal hemopoietic cells and cell linesN A Nicola
Walter and Eliza Hall Institute of Medical Research, P O Royal Melbourne Hospital, Victoria, Australia
Growth Factors 1:41-9. 1988..This resulted in a flexibility of the kinetic parameters that matched the variety of biological responses elicited by CSFs in different cell types... - The combined absence of NF-kappa B1 and c-Rel reveals that overlapping roles for these transcription factors in the B cell lineage are restricted to the activation and function of mature cellsThomas Pohl
The Walter and Eliza Hall Institute of Medical Research, The Royal Melbourne Hospital, Parkville, Victoria 3050, Australia
Proc Natl Acad Sci U S A 99:4514-9. 2002..These findings demonstrate that in the B lineage overlapping roles for NF-kappaB1 and c-Rel appear to be restricted to regulating the activation and function of mature cells... - Clearance and fate of leukemia-inhibitory factor (LIF) after injection into miceD J Hilton
Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Victoria, Australia
J Cell Physiol 148:430-9. 1991..The appearance of a large amount of nonprecipitable 125I in the urine suggested that the kidneys were the major route of LIF clearance from the body... - Synergistic suppression: anomalous inhibition of the proliferation of factor-dependent hemopoietic cells by combination of two colony-stimulating factorsD Metcalf
Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Victoria, Australia
Proc Natl Acad Sci U S A 89:2819-23. 1992..This phenomenon of synergistic suppression may have relevance for the future clinical use of combinations of CSFs, because a potentially similar suppression is also observable with some normal macrophage progenitor cells... - Molecular cloning and expression of the human homologue of the murine gene encoding myeloid leukemia-inhibitory factorN M Gough
Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Victoria, Australia
Proc Natl Acad Sci U S A 85:2623-7. 1988..This factor competed with 125I-labeled native murine LIF for binding to specific cellular receptors on murine cells, compatible with a high degree of structural similarity between the murine and human factors... - Molecular cloning and chromosomal localization of the murine homolog of the human helix-loop-helix gene SCLC G Begley
Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Victoria, Australia
Proc Natl Acad Sci U S A 88:869-73. 1991..The murine SCL homolog was mapped to the central part of chromosome 4... - Absence of yolk sac hematopoiesis from mice with a targeted disruption of the scl geneL Robb
Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Victoria, Australia
Proc Natl Acad Sci U S A 92:7075-9. 1995..Transcripts of GATA-1 and PU.1 transcription factors were absent from RNA from scl-/- yolk sacs and embryos. These results implicate scl as a crucial regulator of early hematopoiesis... - The development of fatal myocarditis and polymyositis in mice heterozygous for IFN-gamma and lacking the SOCS-1 geneD Metcalf
The Walter and Eliza Hall Institute of Medical Research and the Cooperative Research Centre for Cellular Growth Factors, Post Office, Royal Melbourne Hospital, 3050 Victoria, Australia
Proc Natl Acad Sci U S A 97:9174-9. 2000.... - Role of granulocyte-macrophage colony-stimulating factor and granulocyte colony-stimulating factor in the development of an acute neutrophil inflammatory response in miceD Metcalf
Walter and Eliza Hall Institute of Medical Research, Ludwig, Victoria, Australia
Blood 88:3755-64. 1996..Typical eosinophil inflammatory responses to the injection of casein or thioglycollate occurred in GM-CSF -/ -mice but not in beta c -/- mice, suggesting that interleukin-5 was necessary for this response... - Fms-like tyrosine kinase 3 catalytic domain can transduce a proliferative signal in FDC-P1 cells that is qualitatively similar to the signal delivered by c-FmsM T Rossner
Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Parkville, Victoria, Australia
Cell Growth Differ 5:549-55. 1994..abstract truncated at 250 words).. - Leukaemia inhibitory factor and interleukin 6 are expressed at very low levels in the normal adult mouse and are induced by inflammationM A Brown
Walter and Eliza Hall Institute of Medical Research, P O Royal Melbourne Hospital, Parkville, Victoria, Australia
Cytokine 6:300-9. 1994..This induction of LIF and IL-6, by LPS and culturing, may reflect the role of these molecules as mediators of the acute phase response to tissue damage... - Hematopoietic stem cell deficiencies in mice lacking c-Mpl, the receptor for thrombopoietinS Kimura
The Walter and Eliza Hall Institute for Medical Research and The Cooperative Research Centre for Cellular Growth Factors PO Royal Melbourne Hospital, Victoria 3050, Australia
Proc Natl Acad Sci U S A 95:1195-200. 1998..These data imply that TPO, signaling through c-Mpl, plays a vital physiological role in the regulation of hematopoietic stem cell production and function... - A convenient method for preparation of an engineered mouse interleukin-3 analog with high solubility and wild-type bioactivityJames M Murphy
Molecular Medicine Division, The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria 3052, Australia
Growth Factors 28:104-10. 2010..This analog is typically expressed at >1 mg/l of shaking Super broth culture and, owing to solubility >5 mg/ml, structural studies in solution by nuclear magnetic resonance spectroscopy are feasible for mIL-3 for the first time...