Research Topics
Genomes and Genes
| Benjamin T KileSummaryAffiliation: The Walter and Eliza Hall Institute of Medical Research Country: Australia Publications
| Collaborators
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Detail Information
Publications
Mutation discovery in mice by whole exome sequencingHeather Fairfield
The Jackson Laboratory, 600 Main St, Bar Harbor, ME 04609, USA
Genome Biol 12:R86. 2011..We found strong candidate mutations for the majority of mutant exomes sequenced, including new models of orofacial clefting, urogenital dysmorphology, kyphosis and autoimmune hepatitis...- Functional analysis of Asb-1 using genetic modification in miceB T Kile
The Walter and Eliza Hall Institute of Medical Research and the Cooperative Research Centre for Cellular Growth Factors, Royal Melbourne Hospital, Victoria 3050, Australia
Mol Cell Biol 21:6189-97. 2001..In contrast, the widespread overexpression of Asb-1 in the mouse has no apparent deleterious effects... - Mutations in the cofilin partner Aip1/Wdr1 cause autoinflammatory disease and macrothrombocytopeniaBenjamin T Kile
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA
Blood 110:2371-80. 2007..These studies establish an essential requirement for Wdr1 in megakaryocytes and neutrophils, indicating that cofilin-mediated actin dynamics are critically important to the development and function of both cell types... 
The role of the intrinsic apoptosis pathway in platelet life and deathB T Kile
Molecular Medicine Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia
J Thromb Haemost 7:214-7. 2009..This review examines the role of the intrinsic apoptosis pathway, regulated by the Bcl-2 family of proteins, in platelet biology...
The art and design of genetic screens: mouseBenjamin T Kile
Division of Cancer and Hematology, The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville 3050, Victoria, Australia
Nat Rev Genet 6:557-67. 2005....- Negative regulators of cytokine signalingB T Kile
Division of Cancer and Hematology, The Walter and Eliza Hall Institute for Medical Research and The Cooperative Research Centre for Cellular Growth Factors, Royal Melbourne Hospital, Victoria, Australia
Int J Hematol 73:292-8. 2001.... - Cloning and characterization of the genes encoding the ankyrin repeat and SOCS box-containing proteins Asb-1, Asb-2, Asb-3 and Asb-4B T Kile
The Walter and Eliza Hall Institute for Medical Research and The Cooperative Research Centre for Cellular Growth Factors, Post Office, Royal Melbourne Hospital, Melbourne, Victoria 3050, Australia
Gene 258:31-41. 2000..We report here the cloning, characterization, mapping and expression analysis of four members of the ankyrin repeat and SOCS box-containing (Asb) protein family... - Mcl-1 and Bcl-x(L) coordinately regulate megakaryocyte survivalMarlyse A Debrincat
Cancer and Hematology Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, Australia
Blood 119:5850-8. 2012..Our studies indicate that the combination of Bcl-x(L) and Mcl-1 is essential for the viability of the megakaryocyte lineage... - Caspase-9 mediates the apoptotic death of megakaryocytes and platelets, but is dispensable for their generation and functionMichael J White
Cancer and Hematology Division, Walter and Eliza Hall Institute of Medical Research, Parkville, Australia
Blood 119:4283-90. 2012..These studies demonstrate that the apoptotic caspase cascade is required for the efficient death of megakaryocytes and platelets, but is dispensable for their generation and function... - Dual requirement for the ETS transcription factors Fli-1 and Erg in hematopoietic stem cells and the megakaryocyte lineageElizabeth A Kruse
Divisions of Molecular Medicine and Cancer and Haematology, The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville 3052, Australia
Proc Natl Acad Sci U S A 106:13814-9. 2009..These results illustrate an indispensable requirement for both Fli-1 and Erg in normal HSC and megakaryocyte homeostasis, and suggest these transcription factors may coregulate common target genes... - Reduced lymphocyte longevity and homeostatic proliferation in lamin B receptor-deficient mice results in profound and progressive lymphopeniaAnne M Verhagen
Walter and Eliza Hall Institute, Parkville, Victoria 3052, Australia
J Immunol 188:122-34. 2012..In summary, this study demonstrates that LBR deficiency affects not only nuclear architecture but also proliferation, cell viability, and gene expression of hematopoietic cells... - Erg is required for self-renewal of hematopoietic stem cells during stress hematopoiesis in miceAshley P Ng
Walter and Eliza Hall Institute of Medical Research, Parkville, Australia
Blood 118:2454-61. 2011..Thus, Erg is a critical regulator of adult HSCs, essential for maintaining self-renewal at times of high HSC cycling... - Point mutation in the gene encoding p300 suppresses thrombocytopenia in Mpl-/- miceMaria Kauppi
Cancer and Haematology, The Walter and Eliza Hall Institute of Medical Research, Parkville, Australia
Blood 112:3148-53. 2008.... - Critical roles for c-Myb in lymphoid priming and early B-cell developmentKylie T Greig
The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia
Blood 115:2796-805. 2010..These data show that c-Myb plays a critical role in the regulatory pathways governing lymphoid specification and early B-cell differentiation... - Hematopoietic overexpression of the transcription factor Erg induces lymphoid and erythro-megakaryocytic leukemiaCatherine L Carmichael
Cancer and Hematology Division, The Walter and Eliza Hall Institute of Medical Research, Parkville 3052, Vic, Australia
Proc Natl Acad Sci U S A 109:15437-42. 2012..Thus, in mice, Erg overexpression induces the development of not only lymphoid leukemia but also erythro-megakaryocytic leukemia... - Megakaryocytes possess a functional intrinsic apoptosis pathway that must be restrained to survive and produce plateletsEmma C Josefsson
Molecular Medicine Division, Cancer and Hematology Division, The Walter and Eliza Hall Institute of Medical Research, Parkville 3052, Australia Department of Medical Biology, The University of Melbourne, Parkville 3010, Australia
J Exp Med 208:2017-31. 2011..Thus, megakaryocytes do not activate the intrinsic pathway to generate platelets; rather, the opposite is true: they must restrain it to survive and progress safely through proplatelet formation and platelet shedding... - Bcl-xL-inhibitory BH3 mimetics can induce a transient thrombocytopathy that undermines the hemostatic function of plateletsSimone M Schoenwaelder
Australian Centre for Blood Diseases, Monash University, Melbourne, Australia
Blood 118:1663-74. 2011..Overall, these studies demonstrate that Bcl-x(L)-inhibitory BH3 mimetics not only induce thrombocytopenia but also a transient thrombocytopathy that can undermine the hemostatic function of platelets... - Thrombocytopenia and erythrocytosis in mice with a mutation in the gene encoding the hemoglobin β minor chainMaria Kauppi
Cancer and Haematology Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, 3052, Australia
Proc Natl Acad Sci U S A 109:576-81. 2012..The Plt12 mouse is a model of high O(2)-affinity hemoglobinopathy and provides insights into hematopoiesis under conditions of chronic hypoxia... - Trisomy of Erg is required for myeloproliferation in a mouse model of Down syndromeAshley P Ng
Cancer and Haematology Division, The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia
Blood 115:3966-9. 2010.... - An ENU-induced mouse mutant of SHIP1 reveals a critical role of the stem cell isoform for suppression of macrophage activationNhu Y N Nguyen
Rotary Bone Marrow Research Laboratories, Royal Melbourne Hospital, Victoria 3050, Australia
Blood 117:5362-71. 2011..Our results suggest that s-SHIP synergizes with SHIP1 for suppression of macrophage activation, thus providing the first evidence for a role of s-SHIP in adult hematopoiesis... - Ankyrin repeat and suppressors of cytokine signaling box protein asb-9 targets creatine kinase B for degradationMarlyse A Debrincat
Division of Cancer and Haematology, The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria 3050, Australia
J Biol Chem 282:4728-37. 2007..The targeting of CKB for degradation by Asb-9 was primarily SOCS box-dependent and suggests that Asb-9 acts as a specific ubiquitin ligase regulating levels of this evolutionarily conserved enzyme... - The transcription factor Erg is essential for definitive hematopoiesis and the function of adult hematopoietic stem cellsStephen J Loughran
Division of Molecular Medicine, The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3050, Australia
Nat Immunol 9:810-9. 2008..We show that Erg is required for definitive hematopoiesis, adult hematopoietic stem cell function and the maintenance of normal peripheral blood platelet numbers... - A kinase-dead allele of Lyn attenuates autoimmune disease normally associated with Lyn deficiencyAnne M Verhagen
Signal Transduction Laboratory, St Vincent s Institute, Fitzroy, Victoria, Australia
J Immunol 182:2020-9. 2009..Furthermore, our results provide evidence for an additional role for Lyn kinase, distinct from its catalytic activity, in regulating intracellular signaling pathways... - A mouse model of harlequin ichthyosis delineates a key role for Abca12 in lipid homeostasisIan Smyth
Department of Biochemistry and Molecular Biology, Monash University, Melbourne, Australia
PLoS Genet 4:e1000192. 2008..Furthermore, we identify Abca12 as a mediator of Abca1-regulated cellular cholesterol efflux, a finding that may have significant implications for other diseases of lipid metabolism and homeostasis, including atherosclerosis... - Mutational inhibition of c-Myb or p300 ameliorates treatment-induced thrombocytopeniaDouglas J Hilton
Molecular Medicine Division, Walter and Eliza Hall Institute of Medical Research, Parkville, Australia
Blood 113:5599-604. 2009..The data suggest potential utility of c-Myb/p300 as a target for therapeutic intervention in thrombocytopenia of diverse origins... - Agm1/Pgm3-mediated sugar nucleotide synthesis is essential for hematopoiesis and developmentKylie T Greig
Division of Molecular Medicine, The Walter and Eliza Hall Institute of Medical Research, Victoria, Australia
Mol Cell Biol 27:5849-59. 2007.... - Platelet senescence is regulated by an internal timer, not damage inflicted by hitsMark R Dowling
Immunology Division, Walter and Eliza Hall Institute of Medical Research, Parkville, Australia
Blood 116:1776-8. 2010..Thus, at steady state, platelet senescence is probably the product of internal processes rather than external hits... - ERG dependence distinguishes developmental control of hematopoietic stem cell maintenance from hematopoietic specificationSamir Taoudi
Molecular Medicine Division, The Walter and Eliza Institute of Medical Research, Melbourne, Parkville, Victoria 3052, Australia
Genes Dev 25:251-62. 2011..We show that, from the fetal period, ERG acts as a direct upstream regulator of Gata2 and Runx1 gene activity. Without ERG, physiological HSC maintenance fails, leading to the rapid exhaustion of definitive hematopoiesis... - The suppressors of cytokine signalling (SOCS)B T Kile
Division of Cancer and Hematology, The Walter and Eliza Hall Institute for Medical Research and The Cooperative Research Centre for Cellular Growth Factors, Royal Melbourne Hospital, Victoria, Australia
Cell Mol Life Sci 58:1627-35. 2001..Indispensable roles for members of this family have been identified in the regulation of interferon gamma, growth hormone and erythropoietin, and the absence of SOCS-1 or SOCS-3 is lethal in mice... - Programmed anuclear cell death delimits platelet life spanKylie D Mason
Molecular Genetics of Cancer Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3050, Australia
Cell 128:1173-86. 2007.... - A novel mutation in the Nfkb2 gene generates an NF-kappa B2 "super repressor"Elena Tucker
St Vincent s Institute, Fitzroy, Victoria, Australia
J Immunol 179:7514-22. 2007..These findings demonstrate a key role for NF-kappaB2 in the regulation of RelA activation and suggest overlap in the function of NF-kappaB members in canonical and noncanonical pathway signaling... - Two distinct pathways regulate platelet phosphatidylserine exposure and procoagulant functionSimone M Schoenwaelder
Australian Centre for Blood Diseases, Monash University, 89 Commercial Road, Melbourne, Victoria 3004, Australia
Blood 114:663-6. 2009..These studies show the existence of 2 distinct pathways regulating the procoagulant function of platelets... - The SOCS box: a tale of destruction and degradationBenjamin T Kile
The Walter and Eliza Hall Institute of Medical Research and the Cooperative Research Centre for Cellular Growth Factors, Parkville, Victoria, Australia
Trends Biochem Sci 27:235-41. 2002..In this way, SOCS proteins regulate protein turnover by targeting proteins for polyubiquitination and, therefore, for proteasome-mediated degradation... - Platelet life span and apoptosisEmma C Josefsson
Cancer and Haematology Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, Vic, Australia
Methods Mol Biol 788:59-71. 2012..Here, we describe methods to determine platelet life span, enumerate young platelets, and measure hallmarks of platelet apoptosis, such as PS exposure, caspase activation, and mitochondrial dysfunction... - Thrombocytopenia and kidney disease in mice with a mutation in the C1galt1 geneWarren S Alexander
The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria 3050, Australia
Proc Natl Acad Sci U S A 103:16442-7. 2006..The disease in plt1/plt1 mice suggests that, in addition to immune-mediated effects, intrinsic C1Gal-T1 deficiency in megakaryocytes and the kidney may contribute to pathology... - Suppressors of cytokine signaling (SOCS): negative regulators of signal transductionW S Alexander
The Walter and Eliza Hall Institute of Medical Research and the Cooperative Research Centre for Cellular Growth Factors, Royal Melbourne Hospital, Victoria, Australia
J Leukoc Biol 66:588-92. 1999..We propose that the absence of SOCS-1 in these mice prevents lymphocytes and liver cells from appropriately controlling signals from cytokines with cytotoxic side effects... - Probabilistic analysis of recessive mutagenesis screen strategiesJeremy D Silver
Division of Molecular Medicine and the Division of Bioinformatics, The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia
Mamm Genome 18:5-22. 2007..This method allows researchers to contrast, for a range of experimental designs, the cost per mutation screened and to maximize the number of mutations that one can expect to screen in a given experiment... - Apoptotic processes in megakaryocytes and plateletsMichael J White
Molecular Medicine Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia
Semin Hematol 47:227-34. 2010..This review discusses the current state of knowledge about how apoptosis and apoptotic proteins contribute to the generation and function of megakaryocytes and platelets... - Novel roles for erythroid Ankyrin-1 revealed through an ENU-induced null mouse mutantGerhard Rank
Rotary Bone Marrow Research Laboratory, Melbourne Health Research Directorate, Parkville, Australia
Blood 113:3352-62. 2009..These findings provide novel insights into the role of Ank-1, and define an ideal model for the study of HS and malarial resistance... - ENU mutagenesis identifies the first mouse mutants reproducing human β-thalassemia at the genomic levelFiona C Brown
Australian Centre for Blood Diseases, Monash University, Melbourne, Australia Department of Medicine, University of Melbourne, Parkville, Australia
Blood Cells Mol Dis 50:86-92. 2013..The RBC13 and RBC14 lines are the first β-thalassemia mouse models that reproduce human β-thalassemia at the genomic level, and as such highlight the power of ENU mutagenesis screens in generating mouse models of human disease... - Functional genetic analysis of mouse chromosome 11Benjamin T Kile
Department of Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA
Nature 425:81-6. 2003..The mutations reveal new defects in haematopoiesis, craniofacial and cardiovascular development, and fertility... - The role of the ETS factor erg in zebrafish vasculogenesisFelix Ellett
Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, VIC 3050, Australia
Mech Dev 126:220-9. 2009.... - NLRP1 Inflammasome Activation Induces Pyroptosis of Hematopoietic Progenitor CellsSeth L Masters
The Walter and Eliza Hall Institute of Medical Research, Parkville 3052, Australia Department of Medical Biology, The University of Melbourne, Parkville 3010, Australia
Immunity 37:1009-23. 2012..Conversely, NLRP1-deficient mice showed enhanced recovery from chemotherapy and LCMV infection, demonstrating that NLRP1 acts as a cellular sentinel to alert Caspase-1 to hematopoietic and infectious stress... - Genetic mapping of mouse centromere protein (Incenp and Cenpe) genesK J Fowler
The Murdoch Institute, Royal Children s Hospital, Parkville, Victoria, Australia
Cytogenet Cell Genet 82:67-70. 1998..Comparison of Cenpe and human CENPE, which maps to chromosome region 4q24-->q25, has further identified a new region of homology between the two species... - Cloning, expression, and promoter structure of a mammalian inner centromere protein (INCENP)R Saffery
The Murdoch Institute, Royal Children's Hospital, Flemington Road, Parkville, 3052, Victoria, Australia
Mamm Genome 10:415-8. 1999