Michael J Davies

Summary

Affiliation: The Heart Research Institute
Country: Australia

Publications

  1. ncbi request reprint Photo-oxidation of proteins and its role in cataractogenesis
    M J Davies
    The Heart Research Institute, 145 Missenden Road Camperdown, Sydney, NSW 2050, Australia
    J Photochem Photobiol B 63:114-25. 2001
  2. ncbi request reprint Photo-oxidation of cells generates long-lived intracellular protein peroxides
    Adam Wright
    Free Radical Group, Heart Research Institute, Sydney, New South Wales, Australia
    Free Radic Biol Med 34:637-47. 2003
  3. ncbi request reprint The oxidative environment and protein damage
    Michael J Davies
    The Heart Research Institute, 145 Missenden Road, Sydney, NSW 2050, Australia
    Biochim Biophys Acta 1703:93-109. 2005
  4. ncbi request reprint Increased levels of serum protein oxidation and correlation with disease activity in systemic lupus erythematosus
    Philip E Morgan
    Free Radical Group, The Heart Research Institute, Camperdown, Sydney, New South Wales, Australia
    Arthritis Rheum 52:2069-79. 2005
  5. doi request reprint Singlet-oxygen-mediated amino acid and protein oxidation: formation of tryptophan peroxides and decomposition products
    Michelle Gracanin
    The Heart Research Institute, Camperdown, NSW 2050, Australia
    Free Radic Biol Med 47:92-102. 2009
  6. ncbi request reprint Singlet oxygen-mediated damage to proteins and its consequences
    Michael J Davies
    The Heart Research Institute, 145 Missenden Road, Camperdown, Sydney NSW 2050, Australia
    Biochem Biophys Res Commun 305:761-70. 2003
  7. ncbi request reprint Reactive species formed on proteins exposed to singlet oxygen
    Michael J Davies
    The Heart Research Institute, 145 Missenden Road, Camperdown, Sydney, NSW 2050, Australia
    Photochem Photobiol Sci 3:17-25. 2004
  8. ncbi request reprint EPR spin trapping of protein radicals
    Michael J Davies
    The Heart Research Institute, Sydney, Australia
    Free Radic Biol Med 36:1072-86. 2004
  9. ncbi request reprint Hypochlorite-induced oxidation of thiols: formation of thiyl radicals and the role of sulfenyl chlorides as intermediates
    M J Davies
    The Heart Research Institute, Camperdown, New South Wales, Australia
    Free Radic Res 33:719-29. 2000
  10. ncbi request reprint Stable markers of oxidant damage to proteins and their application in the study of human disease
    M J Davies
    The Heart Research Institute, Camperdown, Sydney, NSW, Australia
    Free Radic Biol Med 27:1151-63. 1999

Detail Information

Publications117 found, 100 shown here

  1. ncbi request reprint Photo-oxidation of proteins and its role in cataractogenesis
    M J Davies
    The Heart Research Institute, 145 Missenden Road Camperdown, Sydney, NSW 2050, Australia
    J Photochem Photobiol B 63:114-25. 2001
    ..A particular system is then discussed--the cataractous lens--where UV photo-oxidation may play a role in the aetiology of the disease, and tryptophan-derived metabolites act as UV filters...
  2. ncbi request reprint Photo-oxidation of cells generates long-lived intracellular protein peroxides
    Adam Wright
    Free Radical Group, Heart Research Institute, Sydney, New South Wales, Australia
    Free Radic Biol Med 34:637-47. 2003
    ..These studies demonstrate that exposure of intact cells to visible light in the presence of a sensitizer leads to novel long-lived, but reactive, intracellular protein peroxides via singlet oxygen-mediated reactions...
  3. ncbi request reprint The oxidative environment and protein damage
    Michael J Davies
    The Heart Research Institute, 145 Missenden Road, Sydney, NSW 2050, Australia
    Biochim Biophys Acta 1703:93-109. 2005
    ..The accumulation of such damaged material may contribute to a range of human pathologies...
  4. ncbi request reprint Increased levels of serum protein oxidation and correlation with disease activity in systemic lupus erythematosus
    Philip E Morgan
    Free Radical Group, The Heart Research Institute, Camperdown, Sydney, New South Wales, Australia
    Arthritis Rheum 52:2069-79. 2005
    ..To examine protein oxidation in systemic lupus erythematosus (SLE) and to correlate levels of protein oxidation products with disease activity...
  5. doi request reprint Singlet-oxygen-mediated amino acid and protein oxidation: formation of tryptophan peroxides and decomposition products
    Michelle Gracanin
    The Heart Research Institute, Camperdown, NSW 2050, Australia
    Free Radic Biol Med 47:92-102. 2009
    ..This approach may allow the quantification of protein modification in intact cells arising from singlet oxygen formation...
  6. ncbi request reprint Singlet oxygen-mediated damage to proteins and its consequences
    Michael J Davies
    The Heart Research Institute, 145 Missenden Road, Camperdown, Sydney NSW 2050, Australia
    Biochem Biophys Res Commun 305:761-70. 2003
    ..Such processes can result in the transmittal of damage to other biological targets, and may play a significant role in bystander damage, or dark reactions, in systems where proteins are subjected to oxidation...
  7. ncbi request reprint Reactive species formed on proteins exposed to singlet oxygen
    Michael J Davies
    The Heart Research Institute, 145 Missenden Road, Camperdown, Sydney, NSW 2050, Australia
    Photochem Photobiol Sci 3:17-25. 2004
    ..These secondary reactions, and particularly those involving hydroperoxides, may play a key role in the induction of secondary damage (bystander effects) in systems subject to photo-oxidation...
  8. ncbi request reprint EPR spin trapping of protein radicals
    Michael J Davies
    The Heart Research Institute, Sydney, Australia
    Free Radic Biol Med 36:1072-86. 2004
    ..This article summarizes recent developments in this area and reviews selected examples of radical formation on proteins...
  9. ncbi request reprint Hypochlorite-induced oxidation of thiols: formation of thiyl radicals and the role of sulfenyl chlorides as intermediates
    M J Davies
    The Heart Research Institute, Camperdown, New South Wales, Australia
    Free Radic Res 33:719-29. 2000
    ..The formation of thiyl radicals on oxidation of thiols with HOCl appears to compete with non-radical reactions. The circumstances under which radical formation may be important are discussed...
  10. ncbi request reprint Stable markers of oxidant damage to proteins and their application in the study of human disease
    M J Davies
    The Heart Research Institute, Camperdown, Sydney, NSW, Australia
    Free Radic Biol Med 27:1151-63. 1999
    ....
  11. doi request reprint Tryptophan residues are targets in hypothiocyanous acid-mediated protein oxidation
    Clare L Hawkins
    The Heart Research Institute, 114 Pyrmont Bridge Road, Camperdown, Sydney, NSW 2050, Australia
    Biochem J 416:441-52. 2008
    ..Thus tryptophan residues are likely to be favourable targets for the reaction in biological systems, and the oxidation products formed may be useful biomarkers of HOSCN-mediated protein oxidation...
  12. doi request reprint Cellular effects of photogenerated oxidants and long-lived, reactive, hydroperoxide photoproducts
    Aldwin Suryo Rahmanto
    The Heart Research Institute, Newtown, Sydney, NSW 2042, Australia
    Free Radic Biol Med 49:1505-15. 2010
    ..Overall, this study provides novel insights into early cellular responses to photo-oxidative damage and indicates that long-lived hydroperoxides can play a significant role in cellular damage...
  13. doi request reprint Reduced metal ion concentrations in atherosclerotic plaques from subjects with type 2 diabetes mellitus
    Nadina Stadler
    The Heart Research Institute, Newtown, Sydney, Australia
    Atherosclerosis 222:512-8. 2012
    ..Transition metal ions have been implicated in atherosclerosis. The goal of this study was to investigate whether metal ion levels were higher in people with diabetes, in view of their increased risk of aggravated atherosclerosis...
  14. ncbi request reprint Glycation and glycoxidation of low-density lipoproteins by glucose and low-molecular mass aldehydes. Formation of modified and oxidized particles
    Heather M Knott
    The Heart Research Institute, Camperdown, Australia
    Eur J Biochem 270:3572-82. 2003
    ..This 'carbonyl-stress' may facilitate the formation of foam cells and the vascular complications of diabetes...
  15. pmc Apolipoprotein A-I glycation by glucose and reactive aldehydes alters phospholipid affinity but not cholesterol export from lipid-laden macrophages
    Bronwyn E Brown
    The Heart Research Institute, Sydney, New South Wales, Australia
    PLoS ONE 8:e65430. 2013
    ..Overall these studies demonstrate that glycation of lipid-free apoA-I, particularly late glycation, modifies its structure, its capacity to bind phospholipids and but not ABCA1- or ABCG1-dependent cholesterol efflux from macrophages...
  16. doi request reprint Myeloperoxidase-derived oxidants rapidly oxidize and disrupt zinc-cysteine/histidine clusters in proteins
    Naomi L Cook
    The Heart Research Institute, Newtown, Sydney, NSW 2042, Australia
    Free Radic Biol Med 53:2072-80. 2012
    ..We conclude that exposure of zinc-Cys/His clusters to inflammatory oxidants can result in impaired protein activity, thiol oxidation, and Zn(2+) release. These reactions may contribute to inflammation-induced tissue damage...
  17. doi request reprint Myeloperoxidase-derived oxidants modify apolipoprotein A-I and generate dysfunctional high-density lipoproteins: comparison of hypothiocyanous acid (HOSCN) with hypochlorous acid (HOCl)
    Katrina A Hadfield
    The Heart Research Institute, 7 Eliza St, Newtown, NSW 2042, Australia
    Biochem J 449:531-42. 2013
    ..The ability of HOSCN to impair apoA-I-mediated cholesterol efflux may contribute to the development of atherosclerosis, particularly in smokers who have high plasma levels of SCN- (thiocyanate)...
  18. doi request reprint The nitroxide TEMPO is an efficient scavenger of protein radicals: cellular and kinetic studies
    David I Pattison
    Heart Research Institute, Newtown, Sydney, NSW 2042, Australia
    Free Radic Biol Med 53:1664-74. 2012
    ..were much slower. These data indicate that TEMPO can inhibit secondary reactions of both TyrO. and TrpN., though this is protein dependent. Such protein radical scavenging may contribute to the positive biological effects of nitroxides...
  19. ncbi request reprint Photo-oxidation-induced inactivation of the selenium-containing protective enzymes thioredoxin reductase and glutathione peroxidase
    Aldwin Suryo Rahmanto
    The Heart Research Institute, 7 Eliza Street, Newtown, Sydney, NSW 2042, Australia
    Free Radic Biol Med 53:1308-16. 2012
    ..An oxidant concentration-dependent increase in protein carbonyls was detected with TrxR but not GPx. These studies thus demonstrate that the antioxidant enzymes GPx and TrxR can be irreversibly inactivated by (1)O(2)...
  20. doi request reprint High plasma thiocyanate levels modulate protein damage induced by myeloperoxidase and perturb measurement of 3-chlorotyrosine
    Jihan Talib
    The Heart Research Institute, Newtown, Sydney, NSW 2042, Australia
    Free Radic Biol Med 53:20-9. 2012
    ....
  21. doi request reprint Tryptophan-derived ultraviolet filter compounds covalently bound to lens proteins are photosensitizers of oxidative damage
    Jasminka Mizdrak
    Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, NSW 2109, Australia
    Free Radic Biol Med 44:1108-19. 2008
    ..These data indicate that the covalent modification of lens proteins by Kyn derivatives yields photosensitizers that may enhance oxidation in older lenses and contribute to age-related nuclear cataract...
  22. pmc Degradation of extracellular matrix and its components by hypobromous acid
    Martin D Rees
    The Heart Research Institute, 114 Pyrmont Bridge Road, Sydney, NSW 2050, Australia
    Biochem J 401:587-96. 2007
    ..The degradation of extracellular matrix glycosaminoglycans and proteins by HOBr may contribute to tissue damage associated with inflammatory diseases such as asthma...
  23. doi request reprint Cellular effects of peptide and protein hydroperoxides
    Aldwin Suryo Rahmanto
    The Heart Research Institute, Newtown, Sydney, NSW 2042, Australia
    Free Radic Biol Med 48:1071-8. 2010
    ..This selective oxidation may modulate cellular redox balance and subsequent cell behavior...
  24. doi request reprint Amino acid, peptide, and protein hydroperoxides and their decomposition products modify the activity of the 26S proteasome
    Michelle Gracanin
    Free Radical Group, The Heart Research Institute, Newtown, Sydney, NSW 2042, Australia
    Free Radic Biol Med 50:389-99. 2011
    ..These data indicate that the formation of oxidized proteins within cells may modulate cell function by interfering with the turnover of native proteins and the clearance of modified materials...
  25. doi request reprint Quantification of protein modification by oxidants
    Clare L Hawkins
    The Heart Research Institute, Camperdown, NSW, Australia
    Free Radic Biol Med 46:965-88. 2009
    ..Detailed methods sheets are provided for many of these techniques...
  26. doi request reprint Reactions and reactivity of myeloperoxidase-derived oxidants: differential biological effects of hypochlorous and hypothiocyanous acids
    David I Pattison
    The Heart Research Institute, 7 Eliza Street, Newtown, Sydney, NSW 2042, Australia
    Free Radic Res 46:975-95. 2012
    ....
  27. ncbi request reprint The role of reactive N-bromo species and radical intermediates in hypobromous acid-induced protein oxidation
    Clare L Hawkins
    The Heart Research Institute, 145 Missenden Road, Camperdown, Sydney, NSW 2050, Australia
    Free Radic Biol Med 39:900-12. 2005
    ..With proteins, alpha-carbon (backbone) radicals are detected, which may play a role in protein fragmentation. A novel damage transfer pathway from Gln side-chain amide groups to backbone sites was also observed...
  28. ncbi request reprint Evidence for the formation of adducts and S-(carboxymethyl)cysteine on reaction of alpha-dicarbonyl compounds with thiol groups on amino acids, peptides, and proteins
    Jingmin Zeng
    The Heart Research Institute, 114 Pyrmont Bridge Road, Camperdown, Sydney, NSW 2050, Australia
    Chem Res Toxicol 18:1232-41. 2005
    ..This novel, stable, advanced glycation endproduct is a potential marker of glycation...
  29. pmc Evidence for inactivation of cysteine proteases by reactive carbonyls via glycation of active site thiols
    Jingmin Zeng
    The Heart Research Institute, 145 Missenden Road, Sydney, NSW 2050, Australia
    Biochem J 398:197-206. 2006
    ..This process may contribute to the accumulation of modified proteins in tissues of people with diabetes and age-related pathologies, including atherosclerosis, cataract and Alzheimer's disease...
  30. pmc Hypochlorite and superoxide radicals can act synergistically to induce fragmentation of hyaluronan and chondroitin sulphates
    Martin D Rees
    Free Radical Group, Heart Research Institute, Camperdown, Sydney, NSW 2050, Australia
    Biochem J 381:175-84. 2004
    ..The latter undergo fragmentation reactions in a site-specific manner. This synergistic damage to glycosaminoglycans induced by HOCl and O2*- may be of significance at sites of inflammation where both oxidants are generated concurrently...
  31. doi request reprint Glycosaminoglycans are fragmented by hydroxyl, carbonate, and nitrogen dioxide radicals in a site-selective manner: implications for peroxynitrite-mediated damage at sites of inflammation
    Eleanor C Kennett
    The Heart Research Institute, Camperdown, NSW 2050, Australia
    Free Radic Biol Med 47:389-400. 2009
    ..The time course of formation of these radicals is consistent with these species being involved in polymer fragmentation...
  32. doi request reprint Mechanisms and consequences of oxidative damage to extracellular matrix
    Eleanor C Kennett
    The Heart Research Institute, 7 Eliza Street, Newtown, NSW 2042, Australia
    Biochem Soc Trans 39:1279-87. 2011
    ..The role of such damage in the development and progression of inflammatory diseases is also discussed with particular reference to cardiovascular disease...
  33. doi request reprint What are the plasma targets of the oxidant hypochlorous acid? A kinetic modeling approach
    David I Pattison
    The Heart Research Institute, 114 Pyrmont Bridge Road, Camperdown, Sydney, NSW 2050, Australia
    Chem Res Toxicol 22:807-17. 2009
    ..These observations support the hypothesis that MPO-generated HOSCN is a key agent in smoking-enhanced atherosclerosis...
  34. pmc Myeloperoxidase-derived oxidants selectively disrupt the protein core of the heparan sulfate proteoglycan perlecan
    Martin D Rees
    The Heart Research Institute, 114 Pyrmont Bridge Rd Camperdown, Sydney NSW, Australia
    Matrix Biol 29:63-73. 2010
    ..Collectively, these data highlight the potential role of perlecan oxidation, and consequent deregulation of cell function, in vascular injuries by myeloperoxidase-derived HOCl and HOBr...
  35. doi request reprint High plasma thiocyanate levels in smokers are a key determinant of thiol oxidation induced by myeloperoxidase
    Philip E Morgan
    The Heart Research Institute, Newtown, Sydney, NSW 2042, Australia
    Free Radic Biol Med 51:1815-22. 2011
    ....
  36. doi request reprint Quantification of hydroxyl radical-derived oxidation products in peptides containing glycine, alanine, valine, and proline
    Philip E Morgan
    Free Radical Group, The Heart Research Institute, 7 Eliza Street, Newtown, Sydney, NSW 2042, Australia
    Free Radic Biol Med 52:328-39. 2012
    ..Summation of the product concentrations provides clear evidence for the occurrence of chain reactions in peptides exposed to HO(•)/O(2), with the overall product yields exceeding that of the initial HO(•) generated...
  37. pmc Reaction of protein chloramines with DNA and nucleosides: evidence for the formation of radicals, protein-DNA cross-links and DNA fragmentation
    Clare L Hawkins
    EPR Group, Heart Research Institute, Camperdown, Sydney, New South Wales 2050, Australia
    Biochem J 365:605-15. 2002
    ..Furthermore, the resulting protein chloramines and the radicals derived from them may act as contributing agents in HOCl-mediated DNA oxidation...
  38. ncbi request reprint Protein and low molecular mass thiols as targets and inhibitors of glycation reactions
    Jingmin Zeng
    The Heart Research Institute, 114 Pyrmont Bridge Road, Sydney, NSW 2050, Australia
    Chem Res Toxicol 19:1668-76. 2006
    ..These data may aid the design of therapeutic agents for the treatment of the complications of diabetes...
  39. doi request reprint Nitric oxide and nitroxides can act as efficient scavengers of protein-derived free radicals
    Magdalena A Lam
    The Heart Research Institute, 114 Pyrmont Bridge Road, Camperdown, NSW, Australia
    Chem Res Toxicol 21:2111-9. 2008
    ..These reactions show little dependence on nitroxide structure or charge...
  40. pmc Peroxynitrite modifies the structure and function of the extracellular matrix proteoglycan perlecan by reaction with both the protein core and the heparan sulfate chains
    Eleanor C Kennett
    The Heart Research Institute, 7 Eliza Street, Newtown, NSW 2042, Australia
    Free Radic Biol Med 49:282-93. 2010
    ..These data indicate that peroxynitrite induces major structural and functional changes to perlecan and that damage to this material occurs within human atherosclerotic lesions...
  41. ncbi request reprint The vinyl ether linkages of plasmalogens are favored targets for myeloperoxidase-derived oxidants: a kinetic study
    Ojia Skaff
    The Heart Research Institute, 114 Pyrmont Bridge Road, Camperdown, Sydney, NSW 2050, Australia
    Biochemistry 47:8237-45. 2008
    ....
  42. doi request reprint Identification of plasma proteins that are susceptible to thiol oxidation by hypochlorous acid and N-chloramines
    Fiona A Summers
    The Heart Research Institute, Camperdown, Sydney, NSW 2050, Australia
    Chem Res Toxicol 21:1832-40. 2008
    ..A similar selectivity of reaction and extent of thiol oxidation were also observed with myeloperoxidase in the presence of hydrogen peroxide and chloride ions...
  43. ncbi request reprint Separation, detection, and quantification of hydroperoxides formed at side-chain and backbone sites on amino acids, peptides, and proteins
    Philip E Morgan
    Free Radical Group, The Heart Research Institute, Camperdown, Sydney, NSW 2050, Australia
    Free Radic Biol Med 45:1279-89. 2008
    ..Only side-chain hydroperoxides are detected with free amino acids. These data indicate that free amino acids are poor models of protein damage induced by radicals or other oxidants...
  44. ncbi request reprint Hypothiocyanous acid reactivity with low-molecular-mass and protein thiols: absolute rate constants and assessment of biological relevance
    Ojia Skaff
    The Heart Research Institute, Newtown, Sydney, NSW, Australia
    Biochem J 422:111-7. 2009
    ....
  45. ncbi request reprint Protective mechanisms against peptide and protein peroxides generated by singlet oxygen
    Philip E Morgan
    Free Radical Group, The Heart Research Institute, Camperdown, Sydney, NSW, Australia
    Free Radic Biol Med 36:484-96. 2004
    ....
  46. pmc Inactivation of thiol-dependent enzymes by hypothiocyanous acid: role of sulfenyl thiocyanate and sulfenic acid intermediates
    Tessa J Barrett
    The Heart Research Institute, Newtown, NSW 2042, Australia
    Free Radic Biol Med 52:1075-85. 2012
    ..This is the first direct evidence for the formation of protein sulfenic acids in HOSCN-treated cells and highlights the potential of this oxidant to perturb redox signaling processes...
  47. pmc Myeloperoxidase-derived oxidants inhibit sarco/endoplasmic reticulum Ca2+-ATPase activity and perturb Ca2+ homeostasis in human coronary artery endothelial cells
    Naomi L Cook
    Free Radical Group, The Heart Research Institute, Newtown, NSW 2042, Australia
    Free Radic Biol Med 52:951-61. 2012
    ..MPO-mediated modulation of intracellular Ca(2+) levels may exacerbate endothelial dysfunction, a key early event in atherosclerosis, and be more marked in smokers because of their higher SCN(-) levels...
  48. pmc Selenium-containing amino acids are targets for myeloperoxidase-derived hypothiocyanous acid: determination of absolute rate constants and implications for biological damage
    Ojia Skaff
    The Heart Research Institute, 7 Eliza St, Newtown, Sydney, NSW 2042, Australia
    Biochem J 441:305-16. 2012
    ..These data indicate that selenocysteine residues are oxidized rapidly by HOSCN, with this resulting in the inhibition of the critical intracellular Sec-dependent protective enzymes GPx and TrxR...
  49. ncbi request reprint Concentrations of iron correlate with the extent of protein, but not lipid, oxidation in advanced human atherosclerotic lesions
    Naomi Stanley
    The Heart Research Institute, 145 Missenden Road, Camperdown, Sydney, NSW 2050, Australia
    Free Radic Biol Med 40:1636-43. 2006
    ..These data support the hypothesis that elevated levels of iron contribute to the extent of protein, but not lipid, oxidation in advanced human lesions...
  50. doi request reprint Oxidative damage to extracellular matrix and its role in human pathologies
    Martin D Rees
    The Heart Research Institute, 114 Pyrmont Bridge Rd, Camperdown, NSW 2050, Australia
    Free Radic Biol Med 44:1973-2001. 2008
    ..The role of such damage in the development and progression of inflammatory diseases is discussed...
  51. ncbi request reprint Markers of protein oxidation: different oxidants give rise to variable yields of bound and released carbonyl products
    Henrietta A Headlam
    Free Radical Group, Heart Research Institute, Camperdown, Sydney, Australia
    Free Radic Biol Med 36:1175-84. 2004
    ....
  52. ncbi request reprint Kinetic analysis of the reactions of hypobromous acid with protein components: implications for cellular damage and use of 3-bromotyrosine as a marker of oxidative stress
    David I Pattison
    The Heart Research Institute, 145 Missenden Road, Camperdown, Sydney, NSW 2050, Australia
    Biochemistry 43:4799-809. 2004
    ..Overall, these results shed light on the mechanisms of cell damage induced by HOX and indicate, for example, that the 3-chloro-Tyr:3-bromo-Tyr ratio does not reflect the relative roles of HOCl and HOBr in disease processes...
  53. ncbi request reprint Oxidation of DNA, proteins and lipids by DOPA, protein-bound DOPA, and related catechol(amine)s
    David I Pattison
    The Heart Research Institute, 145 Missenden Road, Camperdown, Sydney, NSW 2050, Australia
    Toxicology 177:23-37. 2002
    ..The exacerbation of damage observed on addition of H(2)O(2) is in accord with a key role for ROS in many of these reactions...
  54. pmc Nonenzymatic glycation impairs the antiinflammatory properties of apolipoprotein A-I
    Estelle Nobecourt
    Lipid Research Group, The Heart Research Institute, Sydney, New South Wales, Australia
    Arterioscler Thromb Vasc Biol 30:766-72. 2010
    ..The goal of this study was to investigate the effects of nonenzymatic glycation on the antiinflammatory properties of apolipoprotein (apo) A-I...
  55. ncbi request reprint Inactivation of protease inhibitors and lysozyme by hypochlorous acid: role of side-chain oxidation and protein unfolding in loss of biological function
    Clare L Hawkins
    The Heart Research Institute, 114 Pyrmont Bridge Road, Camperdown, Sydney, NSW 2050, Australia
    Chem Res Toxicol 18:1600-10. 2005
    ..g., from Lys and His) playing a role in loss of activity...
  56. ncbi request reprint Hypochlorous acid-mediated protein oxidation: how important are chloramine transfer reactions and protein tertiary structure?
    David I Pattison
    The Heart Research Institute, 114 Pyrmont Bridge Road, Camperdown, Sydney, New South Wales 2050, Australia
    Biochemistry 46:9853-64. 2007
    ....
  57. pmc Oxidation of heparan sulphate by hypochlorite: role of N-chloro derivatives and dichloramine-dependent fragmentation
    Martin D Rees
    Free Radical Group, Heart Research Institute, Camperdown, Sydney, NSW 2050, Australia
    Biochem J 391:125-34. 2005
    ..These results suggest that HOCl may be an important mediator of damage to glycosaminoglycans and proteoglycans at inflammatory foci...
  58. ncbi request reprint Kinetic analysis of the role of histidine chloramines in hypochlorous acid mediated protein oxidation
    David I Pattison
    The Heart Research Institute, 145 Missenden Road, Camperdown, Sydney, NSW 2050, Australia
    Biochemistry 44:7378-87. 2005
    ....
  59. ncbi request reprint Sensitizer-mediated photooxidation of histidine residues: evidence for the formation of reactive side-chain peroxides
    Vanessa V Agon
    Free Radical Group, The Heart Research Institute, 145 Missenden Road, Sydney, NSW 2050, Australia
    Free Radic Biol Med 40:698-710. 2006
    ..The latter materials may be partly responsible for the high yield of aggregated materials detected on photooxidation of His-containing proteins...
  60. doi request reprint The smoking-associated oxidant hypothiocyanous acid induces endothelial nitric oxide synthase dysfunction
    Jihan Talib
    Discipline of Pathology, School of Medical Sciences, University of Sydney, Sydney, NSW 2006, Australia
    Biochem J 457:89-97. 2014
    ..Overall these data indicate that high SCN(-) levels, as seen in smokers, can increase HOSCN formation and enhance eNOS dysfunction in human endothelial cells, with this potentially contributing to increased atherogenesis in smokers...
  61. doi request reprint Evidence for chronically elevated serum protein oxidation in systemic lupus erythematosus patients
    Philip E Morgan
    The Heart Research Institute, Camperdown, Sydney, NSW, Australia
    Free Radic Res 43:117-27. 2009
    ..SLE patients with variable disease activity exhibited varying correlations between protein oxidation and disease activity markers. These results further support a role for oxidative stress in the pathogenesis of SLE...
  62. ncbi request reprint Hypochlorous acid-mediated oxidation of lipid components and antioxidants present in low-density lipoproteins: absolute rate constants, product analysis, and computational modeling
    David I Pattison
    The Heart Research Institute, 145 Missenden Road, Camperdown, Sydney, NSW 2050, Australia
    Chem Res Toxicol 16:439-49. 2003
    ..The results from these models compare well with experimental data and can be used to predict the effects of HOCl-mediated oxidation on LDL composition...
  63. doi request reprint Peroxidase catalysed cross-linking of an intrinsically unstructured protein via dityrosine bonds in the oocyst wall of the apicomplexan parasite, Eimeria maxima
    Kelly Mai
    Institute for the Biotechnology of Infectious Diseases, University of Technology, Sydney, NSW 2007, Australia
    Int J Parasitol 41:1157-64. 2011
    ....
  64. ncbi request reprint Beta-scission of side-chain alkoxyl radicals on peptides and proteins results in the loss of side-chains as aldehydes and ketones
    Henrietta A Headlam
    The Free Radical Group, Heart Research Institute, Camperdown, Sydney, Australia
    Free Radic Biol Med 32:1171-84. 2002
    ..The greater than 100% yields confirm the occurrence of chain reactions in peptide/protein oxidation, with more than one residue being damaged per initiating radical...
  65. ncbi request reprint Hypothiocyanous acid is a more potent inducer of apoptosis and protein thiol depletion in murine macrophage cells than hypochlorous acid or hypobromous acid
    Mitchell M Lloyd
    The Heart Research Institute, 114 Pyrmont Bridge Road, Camperdown, Sydney, NSW 2050, Australia
    Biochem J 414:271-80. 2008
    ..This induction of damage by HOSCN may be of critical importance in people with elevated levels of SCN(-) (thiocyanate ions) arising from cigarette smoking, and plays a role in the pathologies associated with this biological insult...
  66. ncbi request reprint Singlet oxygen-mediated protein oxidation: evidence for the formation of reactive side chain peroxides on tyrosine residues
    Adam Wright
    EPR Group, Heart Research Institute, Sydney, NSW, Australia
    Photochem Photobiol 76:35-46. 2002
    ..These studies demonstrate that long-lived Tyr-derived peroxides are formed on proteins exposed to 1O2 and that these may promote damage to other targets via further radical generation...
  67. ncbi request reprint Evidence for rapid inter- and intramolecular chlorine transfer reactions of histamine and carnosine chloramines: implications for the prevention of hypochlorous-acid-mediated damage
    David I Pattison
    The Heart Research Institute, 114 Pyrmont Bridge Road, Camperdown, Sydney, New South Wales 2050, Australia
    Biochemistry 45:8152-62. 2006
    ....
  68. doi request reprint Accumulation of zinc in human atherosclerotic lesions correlates with calcium levels but does not protect against protein oxidation
    Nadina Stadler
    The Heart Research Institute, 114 Pyrmont Bridge Road, Camperdown, Sydney, NSW 2050, Australia
    Arterioscler Thromb Vasc Biol 28:1024-30. 2008
    ....
  69. ncbi request reprint Inhibition of protein tyrosine phosphatases by amino acid, peptide, and protein hydroperoxides: potential modulation of cell signaling by protein oxidation products
    Michelle Gracanin
    The Heart Research Institute, 114 Pyrmont Bridge Road, Camperdown, NSW 2050, Australia
    Free Radic Biol Med 42:1543-51. 2007
    ..This inhibition of PTPs, by oxidized proteins containing hydroperoxide groups, may contribute to cellular dysfunction and altered redox signaling in systems subject to oxidative stress...
  70. pmc Effect of exposure of human monocyte-derived macrophages to high, versus normal, glucose on subsequent lipid accumulation from glycated and acetylated low-density lipoproteins
    Fatemeh Moheimani
    Free Radical Group, The Heart Research Institute, 7 Eliza Street, Newtown, Sydney, NSW 2042, Australia
    Exp Diabetes Res 2011:851280. 2011
    ..It is concluded that, whilst macrophage scavenger receptor mRNA and protein levels can be modulated by high glucose, these are not key factors in lipid accumulation by human macrophages under the conditions examined...
  71. ncbi request reprint Glycation of low-density lipoprotein results in the time-dependent accumulation of cholesteryl esters and apolipoprotein B-100 protein in primary human monocyte-derived macrophages
    Bronwyn E Brown
    Free Radical Group, The Heart Research Institute, 114 Pyrmont Bridge Road, Camperdown, Sydney, NSW 2050, Australia
    FEBS J 274:1530-41. 2007
    ....
  72. ncbi request reprint Degradation of extracellular matrix by peroxynitrite/peroxynitrous acid
    Eleanor C Kennett
    The Heart Research Institute, 114 Pyrmont Bridge Road, Camperdown, NSW 2050, Australia
    Free Radic Biol Med 45:716-25. 2008
    ..These reactions may contribute to ECM damage at sites of inflammation and play a role in disease progression, including rupture of atherosclerotic lesions...
  73. doi request reprint One-electron reduction of N-chlorinated and N-brominated species is a source of radicals and bromine atom formation
    David I Pattison
    The Heart Research Institute, Newtown, NSW, Australia
    Chem Res Toxicol 24:371-82. 2011
    ..These data suggest that interaction of N-halogenated species with O(2)(•-) may produce deleterious N-centered radicals and Br(•)...
  74. pmc Fragmentation of extracellular matrix by hypochlorous acid
    Alan A Woods
    The Heart Research Institute, 145 Missenden Road, Camperdown, Sydney, New South Wales 2050, Australia
    Biochem J 376:219-27. 2003
    ..The observed matrix modifications reported here may therefore modulate cellular behaviour in diseases such as atherosclerosis where MPO-derived oxidants are generated...
  75. ncbi request reprint Kinetics of hypobromous acid-mediated oxidation of lipid components and antioxidants
    Ojia Skaff
    The Heart Research Institute, Sydney, Australia
    Chem Res Toxicol 20:1980-8. 2007
    ..The data obtained indicate that HOBr reacts to a much greater extent with fatty acid side chains and lipid-soluble antioxidants than HOCl; this has important implications for HOBr-mediated damage to cells and lipoproteins...
  76. ncbi request reprint Cell-mediated reduction of protein and peptide hydroperoxides to reactive free radicals
    Henrietta A Headlam
    Free Radical Group, The Heart Research Institute, Sydney, NSW, Australia
    Free Radic Biol Med 34:44-55. 2003
    ....
  77. ncbi request reprint Hypochlorite-induced oxidation of amino acids, peptides and proteins
    C L Hawkins
    The Heart Research Institute, Free Radical Group, Camperdown, Sydney, NSW, Australia
    Amino Acids 25:259-74. 2003
    ..The predicted targets for reaction with HOCl from kinetic modeling studies and the consequences of HOCl-induced protein oxidation are also discussed...
  78. ncbi request reprint Comparative time-courses of copper-ion-mediated protein and lipid oxidation in low-density lipoprotein
    Heather M Knott
    Cell Biology and EPR Groups, The Heart Research Institute, 145 Missenden Road, Camperdown, NSW 2050, Australia
    Arch Biochem Biophys 400:223-32. 2002
    ....
  79. ncbi request reprint Hypochlorite-induced damage to DNA, RNA, and polynucleotides: formation of chloramines and nitrogen-centered radicals
    Clare L Hawkins
    The EPR Group, Heart Research Institute, Camperdown, Sydney, New South Wales 2050, Australia
    Chem Res Toxicol 15:83-92. 2002
    ..Overall the data rationalize the preferential formation of chlorinated 2'-deoxycytidine and 2'-deoxyadenosine in DNA and suggest that DNA damage induced by HOCl, and preformed chloramines, occurs at sequence-specific sites...
  80. ncbi request reprint Absolute rate constants for the reaction of hypochlorous acid with protein side chains and peptide bonds
    D I Pattison
    The Heart Research Institute, 145 Missenden Road, Camperdown, Sydney, NSW 2050, Australia
    Chem Res Toxicol 14:1453-64. 2001
    ..These models are useful tools for predicting, and reconciling, experimental data obtained in HOCl-induced oxidations and allow estimations to be made as to the flux of HOCl to which proteins are exposed in vivo...
  81. ncbi request reprint Mammalian heme peroxidases: from molecular mechanisms to health implications
    Michael J Davies
    The Heart Research Institute, Camperdown, University of Sydney, Sydney, Australia, Faculty of Medicine, University of Sydney, Sydney, Australia
    Antioxid Redox Signal 10:1199-234. 2008
    ....
  82. doi request reprint Acetaminophen (paracetamol) inhibits myeloperoxidase-catalyzed oxidant production and biological damage at therapeutically achievable concentrations
    Maud Koelsch
    Heart Research Institute, Newtown, Sydney, NSW, Australia
    Biochem Pharmacol 79:1156-64. 2010
    ..Overall, these data indicate that acetaminophen may be an important modulator of MPO activity in vivo...
  83. ncbi request reprint The role of aromatic amino acid oxidation, protein unfolding, and aggregation in the hypobromous acid-induced inactivation of trypsin inhibitor and lysozyme
    Clare L Hawkins
    The Heart Research Institute, Camperdown, Sydney, NSW 2050, Australia
    Chem Res Toxicol 18:1669-77. 2005
    ..Decomposition of these species correlates with secondary oxidation reactions, and with lysozyme, a time-dependent loss in activity. Overall, 70% of the HOBr can be accounted for with STI and 95% with lysozyme...
  84. pmc Inhibition of myeloperoxidase-mediated hypochlorous acid production by nitroxides
    Martin D Rees
    The Heart Research Institute, 114 Pyrmont Bridge Rd, Camperdown, Sydney, NSW 2050, Australia
    Biochem J 421:79-86. 2009
    ..Overall, these data indicate that nitroxides have considerable promise as therapeutic agents for the inhibition of MPO-mediated damage in inflammatory diseases...
  85. ncbi request reprint Serum protein oxidation and apolipoprotein CIII levels in people with systemic lupus erythematosus with and without nephritis
    Philip E Morgan
    Free Radical Group, The Heart Research Institute, Camperdown, Sydeny, Australia
    Free Radic Res 41:1301-12. 2007
    ..Total apoCIII levels (assessed by ELISA) in lupus nephritis patients were significantly elevated compared to controls or non-renal SLE patients. Thus, levels of oxidized proteins and apoCIII may be useful biomarkers in SLE studies...
  86. ncbi request reprint Generation and propagation of radical reactions on proteins
    C L Hawkins
    Heart Research Institute, 145 Missenden Road, Camperdown, 2050, Sydney, NSW, Australia
    Biochim Biophys Acta 1504:196-219. 2001
    ..The final section of this review examines the control of protein oxidation and how such damage might be limited by antioxidants...
  87. ncbi request reprint Reactions of myeloperoxidase-derived oxidants with biological substrates: gaining chemical insight into human inflammatory diseases
    D I Pattison
    The Heart Research Institute, 114 Pyrmont Bridge Rd, Camperdown, Sydney, NSW 2050, Australia
    Curr Med Chem 13:3271-90. 2006
    ....
  88. ncbi request reprint Heparan sulfate degradation via reductive homolysis of its N-chloro derivatives
    Martin D Rees
    Heart Research Institute, 145 147 Missenden Road, Camperdown, Sydney NSW 2050, Australia
    J Am Chem Soc 128:3085-97. 2006
    ....
  89. ncbi request reprint Inhibition of glyceraldehyde-3-phosphate dehydrogenase by peptide and protein peroxides generated by singlet oxygen attack
    Philip E Morgan
    EPR Group, The Heart Research Institute, Sydney, New South Wales, Australia
    Eur J Biochem 269:1916-25. 2002
    ..These reactions may be important in the development of cellular dysfunction as a result of photo-oxidation...
  90. ncbi request reprint Analysis of aliphatic amino acid alcohols in oxidized proteins
    Bénédicte Morin
    Heart Research Institute, Sydney, Australia
    Methods Mol Biol 186:101-10. 2002
  91. ncbi request reprint Induction of DNA damage by oxidised amino acids and proteins
    Catherine Luxford
    The Heart Research Institute, Sydney, NSW, Australia
    Biogerontology 3:95-102. 2002
    ..These radicals are shown to react with DNA to give DNA-protein cross-links and single strand breaks...
  92. pmc Detection of HOCl-mediated protein oxidation products in the extracellular matrix of human atherosclerotic plaques
    Alan A Woods
    The Heart Research Institute, 145 Missenden Road, Camperdown, Sydney, New South Wales 2050, Australia
    Biochem J 370:729-35. 2003
    ..This is believed to involve the formation and subsequent decomposition of protein chloramines...
  93. ncbi request reprint Reversible binding of kynurenine to lens proteins: potential protection by glutathione in young lenses
    Nicole R Parker
    Save Sight Institute, University of Sydney, Sydney, NSW, Australia
    Invest Ophthalmol Vis Sci 48:3705-13. 2007
    ..The aim of this study was to examine in vitro rates of formation and decomposition of the three major Kyn-amino acid adducts and possible consequences for the lens...
  94. ncbi request reprint The retina: oxidative stress and diabetes
    David M van Reyk
    Department of Health Sciences, University of Technology, Sydney, New South Wales, Australia
    Redox Rep 8:187-92. 2003
    ....
  95. ncbi request reprint Hypochlorite- and hypobromite-mediated radical formation and its role in cell lysis
    C L Hawkins
    The Heart Research Institute, Camperdown, Sydney, New South Wales 2050, Australia
    Arch Biochem Biophys 395:137-45. 2001
    ..Overall these experiments demonstrate that HOCl and HOBr react with different selectivity with cellular targets, and that this can result in radical formation. This radical generation can precede, and may play a role in, cell lysis...
  96. ncbi request reprint Superoxide radicals can act synergistically with hypochlorite to induce damage to proteins
    Clare L Hawkins
    The EPR Group, Heart Research Institute, Camperdown, Sydney, NSW 2050, Australia
    FEBS Lett 510:41-4. 2002
    ..This synergistic action may be of significance at sites of inflammation, where both superoxide radicals and chloramines/chloramides are formed simultaneously...
  97. ncbi request reprint Hypochlorite-mediated fragmentation of hyaluronan, chondroitin sulfates, and related N-acetyl glycosamines: evidence for chloramide intermediates, free radical transfer reactions, and site-specific fragmentation
    Martin D Rees
    Heart Research Institute, 145 Missenden Road, Camperdown, Sydney NSW 2050, Australia
    J Am Chem Soc 125:13719-33. 2003
    ....
  98. pmc The myeloperoxidase-derived oxidant HOSCN inhibits protein tyrosine phosphatases and modulates cell signalling via the mitogen-activated protein kinase (MAPK) pathway in macrophages
    Amanda E Lane
    The Heart Research Institute, Newtown, Sydney, NSW, Australia
    Biochem J 430:161-9. 2010
    ..These changes occur with (patho)physiological concentrations of SCN- ions, and implicate HOSCN as an important mediator of inflammation-induced oxidative damage, particularly in smokers who have elevated plasma levels of SCN-...
  99. ncbi request reprint The cytoplasmic Cu,Zn superoxide dismutase of saccharomyces cerevisiae is required for resistance to freeze-thaw stress. Generation of free radicals during freezing and thawing
    J I Park
    School of Biochemistry and Molecular Genetics and Cooperative Research Center for Food Industry Innovation, University of New South Wales, Sydney, New South Wales 2052, Australia
    J Biol Chem 273:22921-8. 1998
    ....
  100. ncbi request reprint Formation of hydroxyl radicals in the human lens is related to the severity of nuclear cataract
    B Garner
    Australian Cataract Research Foundation, Department of Chemistry, University of Wollongong, Wollongong, NSW, 2522, Australia
    Exp Eye Res 70:81-8. 2000
    ..The levels of both metals were lower in non-cataractous lenses. These data support the hypothesis that transition metal-mediated HO. production may play a role in the aetiology of age-related nuclear cataract...
  101. ncbi request reprint Oxidation and antioxidation of human low-density lipoprotein and plasma exposed to 3-morpholinosydnonimine and reagent peroxynitrite
    S R Thomas
    The Biochemistry and EPR Groups, The Heart Research Institute, 145 Missenden Road, Camperdown, Sydney, NSW 2050, Australia
    Chem Res Toxicol 11:484-94. 1998
    ..The studies also highlight differences between ONOO-- and SIN-1-induced LDL oxidation with regards to the effects of bicarbonate, ascorbate, and urate...