S M Pitson

Summary

Affiliation: South Australia
Country: Australia

Publications

  1. ncbi request reprint Sphingosine kinase interacts with TRAF2 and dissects tumor necrosis factor-alpha signaling
    Pu Xia
    Division of Human Immunology, The Hanson Institute, Institute of Medical and Veterinary Science and University of Adelaide, Frome Road, Adelaide SA 5000, Australia
    J Biol Chem 277:7996-8003. 2002
  2. pmc Phosphorylation-dependent translocation of sphingosine kinase to the plasma membrane drives its oncogenic signalling
    Stuart M Pitson
    Hanson Institute and Division of Human Immunology, Institute of Medical and Veterinary Science, Adelaide SA 5000, Australia
    J Exp Med 201:49-54. 2005
  3. ncbi request reprint A point mutant of human sphingosine kinase 1 with increased catalytic activity
    S M Pitson
    Hanson Centre for Cancer Research, Division of Human Immunology, Institute of Medical and Veterinary Science, Frome Road, Adelaide, SA 5000, Australia
    FEBS Lett 509:169-73. 2001
  4. pmc Science amongst the vines. Meeting on signalling systems
    Stuart M Pitson
    Molecular Signalling Laboratory, Hanson Institute and Division of Human Immunology, Institute of Medical and Veterinary Science, Frome Road, Adelaide 5000, Australia
    EMBO Rep 9:425-8. 2008
  5. pmc Activation of sphingosine kinase 1 by ERK1/2-mediated phosphorylation
    Stuart M Pitson
    Hanson Institute, Division of Human Immunology, Institute of Medical and Veterinary Science, Frome Road, Adelaide, SA 5000, Australia
    EMBO J 22:5491-500. 2003
  6. ncbi request reprint An oncogenic role of sphingosine kinase
    P Xia
    Division of Human Immunology, Hanson Centre for Cancer Research, Institute of Medical and Veterinary Science and University of Adelaide, Frome Road, SA 5000, Adelaide, Australia
    Curr Biol 10:1527-30. 2000
  7. pmc Basal and angiopoietin-1-mediated endothelial permeability is regulated by sphingosine kinase-1
    Xiaochun Li
    Division of Human Immunology, Hanson Institute, Adelaide, Australia
    Blood 111:3489-97. 2008
  8. pmc Human sphingosine kinase: purification, molecular cloning and characterization of the native and recombinant enzymes
    S M Pitson
    Hanson Centre for Cancer Research, Division of Human Immunology, Institute of Medical and Veterinary Science, Frome Road, Adelaide 5000, SA, Australia
    Biochem J 350:429-41. 2000
  9. ncbi request reprint Activation of endothelial extracellular signal-regulated kinase is essential for neutrophil transmigration: potential involvement of a soluble neutrophil factor in endothelial activation
    Brian N Stein
    Hanson Institute, Institute of Medical and Veterinary Science, and Department of Medicine, University of Adelaide, Adelaide, Australia
    J Immunol 171:6097-104. 2003
  10. pmc Eukaryotic elongation factor 1A interacts with sphingosine kinase and directly enhances its catalytic activity
    Tamara M Leclercq
    Hanson Institute, Division of Human Immunology, Institute of Medical and Veterinary Science, Frome Road, Adelaide, South Australia 5000, Australia
    J Biol Chem 283:9606-14. 2008

Collaborators

Detail Information

Publications39

  1. ncbi request reprint Sphingosine kinase interacts with TRAF2 and dissects tumor necrosis factor-alpha signaling
    Pu Xia
    Division of Human Immunology, The Hanson Institute, Institute of Medical and Veterinary Science and University of Adelaide, Frome Road, Adelaide SA 5000, Australia
    J Biol Chem 277:7996-8003. 2002
    ..These findings show a role for SphK in the signal transduction by TRAF2 specifically leading to activation of NF-kappa B and antiapoptosis...
  2. pmc Phosphorylation-dependent translocation of sphingosine kinase to the plasma membrane drives its oncogenic signalling
    Stuart M Pitson
    Hanson Institute and Division of Human Immunology, Institute of Medical and Veterinary Science, Adelaide SA 5000, Australia
    J Exp Med 201:49-54. 2005
    ..Thus, phosphorylation of hSK1 is essential for oncogenic signaling, and is brought about through phosphorylation-induced translocation of hSK1 to the plasma membrane, rather than from enhanced catalytic activity of this enzyme...
  3. ncbi request reprint A point mutant of human sphingosine kinase 1 with increased catalytic activity
    S M Pitson
    Hanson Centre for Cancer Research, Division of Human Immunology, Institute of Medical and Veterinary Science, Frome Road, Adelaide, SA 5000, Australia
    FEBS Lett 509:169-73. 2001
    ..Interestingly, hSK(G113A) activity could be stimulated in HEK293T cells by cell agonists to a comparable extent to hSK(WT)...
  4. pmc Science amongst the vines. Meeting on signalling systems
    Stuart M Pitson
    Molecular Signalling Laboratory, Hanson Institute and Division of Human Immunology, Institute of Medical and Veterinary Science, Frome Road, Adelaide 5000, Australia
    EMBO Rep 9:425-8. 2008
  5. pmc Activation of sphingosine kinase 1 by ERK1/2-mediated phosphorylation
    Stuart M Pitson
    Hanson Institute, Division of Human Immunology, Institute of Medical and Veterinary Science, Frome Road, Adelaide, SA 5000, Australia
    EMBO J 22:5491-500. 2003
    ....
  6. ncbi request reprint An oncogenic role of sphingosine kinase
    P Xia
    Division of Human Immunology, Hanson Centre for Cancer Research, Institute of Medical and Veterinary Science and University of Adelaide, Frome Road, SA 5000, Adelaide, Australia
    Curr Biol 10:1527-30. 2000
    ..The findings not only point to a new signalling pathway in transformation but also to the potential of SphK inhibitors in cancer therapy...
  7. pmc Basal and angiopoietin-1-mediated endothelial permeability is regulated by sphingosine kinase-1
    Xiaochun Li
    Division of Human Immunology, Hanson Institute, Adelaide, Australia
    Blood 111:3489-97. 2008
    ..In addition, the Ang-1-induced inhibition of leakiness is mediated through activation of SK-1, defining a new signaling pathway in the Ang-1 regulation of permeability...
  8. pmc Human sphingosine kinase: purification, molecular cloning and characterization of the native and recombinant enzymes
    S M Pitson
    Hanson Centre for Cancer Research, Division of Human Immunology, Institute of Medical and Veterinary Science, Frome Road, Adelaide 5000, SA, Australia
    Biochem J 350:429-41. 2000
    ..In all cases, both the native and recombinant SKs displayed remarkably similar properties, indicating that post-translational modifications are not required for basal activity of human SK...
  9. ncbi request reprint Activation of endothelial extracellular signal-regulated kinase is essential for neutrophil transmigration: potential involvement of a soluble neutrophil factor in endothelial activation
    Brian N Stein
    Hanson Institute, Institute of Medical and Veterinary Science, and Department of Medicine, University of Adelaide, Adelaide, Australia
    J Immunol 171:6097-104. 2003
    ..Further characterization showed that the trigger for endothelial ERK activation is a soluble protein of molecular mass approximately 30 kDa released from neutrophils after activation...
  10. pmc Eukaryotic elongation factor 1A interacts with sphingosine kinase and directly enhances its catalytic activity
    Tamara M Leclercq
    Hanson Institute, Division of Human Immunology, Institute of Medical and Veterinary Science, Frome Road, Adelaide, South Australia 5000, Australia
    J Biol Chem 283:9606-14. 2008
    ..Thus, this study has established a novel mechanism of regulation of both SK1 and SK2 that is mediated by their interaction with eEF1A...
  11. ncbi request reprint The nucleotide-binding site of human sphingosine kinase 1
    Stuart M Pitson
    Hanson Institute, Division of Human Immunology, Institute of Medical and Veterinary Science, Frome Road, Adelaide SA 5000, Australia
    J Biol Chem 277:49545-53. 2002
    ..It does, however, share some sequence and likely structural similarity with the highly conserved glycine-rich loop, which is known to be involved in anchoring and positioning the nucleotide in the catalytic site of many protein kinases...
  12. ncbi request reprint The calmodulin-binding site of sphingosine kinase and its role in agonist-dependent translocation of sphingosine kinase 1 to the plasma membrane
    Catherine M Sutherland
    Hanson Institute, Division of Human Immunology, Institute of Medical and Veterinary Science, Frome Road, University of Adelaide, Adelaide, South Australia 5000, Australia
    J Biol Chem 281:11693-701. 2006
    ....
  13. doi request reprint Reduced sphingosine kinase 1 activity in dengue virus type-2 infected cells can be mediated by the 3' untranslated region of dengue virus type-2 RNA
    J M Carr
    Flinders Medical Science and Technology, School of Medicine, Flinders University, University Drive, Bedford Park, Adelaide, South Australia 5042, Australia
    J Gen Virol 94:2437-48. 2013
    ..Nonetheless, reduced SphK1 activity may have important consequences for survival or death of the infected cell...
  14. pmc Sphingosine kinase regulates the rate of endothelial progenitor cell differentiation
    Claudine S Bonder
    Division of Human Immunology, Hanson Institute, Institute of Medical and Veterinary Science, Adelaide, South Australia, Australia
    Blood 113:2108-17. 2009
    ..Thus, SK-1 is a critical player in EPC differentiation into EC pointing to the potential utility of SK-1 modifying agents in the specific manipulation of endothelial development and repair...
  15. ncbi request reprint Chronic increases in sphingosine kinase-1 activity induce a pro-inflammatory, pro-angiogenic phenotype in endothelial cells
    Vidya Limaye
    Royal Adelaide Hospital, Department of Rheumatology, North Tce, Royal Adelaide Hospital, Adelaide, SA, 5000, Australia
    Cell Mol Biol Lett 14:424-41. 2009
    ..The SK1 signaling pathway is activated in RA, suggesting that manipulation of SK1 activity in diseases of aberrant inflammation and angiogenesis may be beneficial...
  16. ncbi request reprint The effects of markedly raised intracellular sphingosine kinase-1 activity in endothelial cells
    Vidya Limaye
    Royal Adelaide Hospital, Department of Rheumatology, North Tce, Royal Adelaide Hospital, Adelaide, SA, 5000, Australia
    Cell Mol Biol Lett 14:411-23. 2009
    ..Our results suggest that SK1 activity is subject to control in the EC, and that this control may be lost in conditions involving vascular regression...
  17. doi request reprint Regulation of sphingosine kinase and sphingolipid signaling
    Stuart M Pitson
    Centre for Cancer Biology, SA Pathology, Frome Road, Adelaide SA 5000, Australia
    Trends Biochem Sci 36:97-107. 2011
    ....
  18. ncbi request reprint Meeting report: Barossa 2005--Signaling Networks
    Mark A Guthridge
    Department of Human Immunology, Institute of Medical and Veterinary Science, Adelaide, South Australia 5000, Australia
    Sci STKE 2006:pe9. 2006
    ....
  19. doi request reprint Sphingosine and FTY720 directly bind pro-survival 14-3-3 proteins to regulate their function
    Joanna M Woodcock
    Division of Human Immunology, Centre for Cancer Biology, SA Pathology, Adelaide, SA 5000, Australia
    Cell Signal 22:1291-9. 2010
    ..Furthermore, these results suggest a new therapeutic activity for FTY720 as an anti-cancer agent based on this mechanism...
  20. ncbi request reprint Molecular targets of FTY720 (fingolimod)
    M R Pitman
    Centre for Cancer Biology, SA Pathology, Frome Road, Adelaide SA 5000, Australia
    Curr Mol Med 12:1207-19. 2012
    ..We also discuss other recently described protein effectors that, in combination with S1P receptors, appear to contribute to its immunosuppressive effects...
  21. doi request reprint Guanine nucleotides regulate sphingosine kinase 1 activation by eukaryotic elongation factor 1A and provide a mechanism for eEF1A-associated oncogenesis
    T M Leclercq
    Molecular Signalling Laboratory, Centre for Cancer Biology, SA Pathology, Adelaide, South Australia, Australia
    Oncogene 30:372-8. 2011
    ..Thus, this study defines the mechanism regulating eEF1A-mediated SK1 activation, and also establishes SK1 as being integral for PTI-1-induced oncogenesis...
  22. pmc Deactivation of sphingosine kinase 1 by protein phosphatase 2A
    Renae K Barr
    Hanson Institute, Division of Human Immunology, Institute of Medical and Veterinary Science, University of Adelaide, Adelaide, Australia
    J Biol Chem 283:34994-5002. 2008
    ..Finally, both basal and tumor necrosis factor-alpha-stimulated cellular SK1 activity were regulated by molecular manipulation of PP2Ac activity. Thus, PP2A appears to function as an endogenous regulator of SK1 phosphorylation...
  23. doi request reprint The CCT/TRiC chaperonin is required for maturation of sphingosine kinase 1
    Julia R Zebol
    Hanson Institute, Division of Human Immunology, Institute of Medical and Veterinary Science, Frome Road, Adelaide, SA 5000, Australia
    Int J Biochem Cell Biol 41:822-7. 2009
    ..Thus, combined this data suggests that SK1 is a CCT/TRiC substrate, and that this chaperonin facilitates folding of newly translated SK1 into its mature active form...
  24. pmc Translocation of sphingosine kinase 1 to the plasma membrane is mediated by calcium- and integrin-binding protein 1
    Kate E Jarman
    Centre for Cancer Biology, SA Pathology, Frome Road, Adelaide, South Australia, Australia
    J Biol Chem 285:483-92. 2010
    ..Furthermore, we demonstrate the requirement of CIB1-mediated translocation of SK1 in controlling cellular sphingosine 1-phosphate generation and associated anti-apoptotic signaling...
  25. doi request reprint A proinflammatory role for proteolytically cleaved annexin A1 in neutrophil transendothelial migration
    Samantha L Williams
    Division of Human Immunology, Centre for Cancer Biology, SA Pathology, Adelaide, South Australia, Australia
    J Immunol 185:3057-63. 2010
    ..We also provide evidence that C-terminal AnxA1 functions by increasing ICAM1 clustering around adherent neutrophils to anchor them to the endothelium and promote transmigration through the transcellular route...
  26. ncbi request reprint Sphingosine kinase transmits estrogen signaling in human breast cancer cells
    Olga A Sukocheva
    Signal Transduction Laboratory, Division of Human Immunology, Hanson Institute, Institute of Medical and Veterinary Science and University of Adelaide, Adelaide, South Australia 5000, Australia
    Mol Endocrinol 17:2002-12. 2003
    ..Thus, our data suggest that SphK activation is an important cytoplasmic signaling to transduce estrogen-dependent mitogenic and carcinogenic action in human breast cancer cells...
  27. ncbi request reprint An assay for sphingosine kinase activity using biotinylated sphingosine and streptavidin-coated membranes
    Jane L Roberts
    Hanson Institute, Division of Human Immunology, Institute of Medical and Veterinary Science, Frome Road, Adelaide, SA 5000, Australia
    Anal Biochem 331:122-9. 2004
    ..Together, these results demonstrate the potential utility of this assay in both cell-based analysis of sphingosine kinase signaling pathways and high-throughput screens for agents affecting sphingosine kinase activity in vitro...
  28. ncbi request reprint Cellular signalling by sphingosine kinase and sphingosine 1-phosphate
    Tamara M Leclercq
    Hanson Institute, Division of Human Immunology, Institute of Medical and Veterinary Science, Adelaide, Australia
    IUBMB Life 58:467-72. 2006
    ....
  29. ncbi request reprint Regulation of stem cell pluripotency and neural differentiation by lysophospholipids
    Stuart M Pitson
    Division of Human Immunology, Centre for Cancer Biology, SA Pathology, University of Adelaide, Adelaide, S A, Australia
    Neurosignals 17:242-54. 2009
    ..This review aims to summarise the current knowledge on LPA and S1P regulation of embryonic and neural stem cell biology...
  30. pmc Tumor necrosis factor-induced neutrophil adhesion occurs via sphingosine kinase-1-dependent activation of endothelial {alpha}5{beta}1 integrin
    Wai Y Sun
    Human Immunology, Centre for Cancer Biology, SA Pathology, Frome Rd, Adelaide, SA 5000, Australia
    Am J Pathol 177:436-46. 2010
    ..Moreover, this work supports the notion that sphingosine kinase-1 may be the single target required for an effective broad spectrum approach to combat inflammation and immune disorders...
  31. ncbi request reprint Inhibitors of the sphingosine kinase pathway as potential therapeutics
    Melissa R Pitman
    Molecular Signalling Laboratory, Centre for Cancer Biology, SA Pathology, Frome Road, Adelaide, Australia
    Curr Cancer Drug Targets 10:354-67. 2010
    ..Furthermore, we highlight findings demonstrating the success of SK inhibition in cancer and a range of other disease models that promotes the continued interest in targeting the SKs for therapeutic benefit...
  32. ncbi request reprint The localization and activity of sphingosine kinase 1 are coordinately regulated with actin cytoskeletal dynamics in macrophages
    David J Kusner
    Inflammation Program, Division of Infectious Diseases, Department of Internal Medicine, University of Iowa Carver College of Medicine and Veterans Affairs Medical Center, Iowa City, Iowa 52245, USA
    J Biol Chem 282:23147-62. 2007
    ..Thus, the localization and activity of SK1 are coordinately regulated with actin dynamics during macrophage activation...
  33. ncbi request reprint Sphingosine 1-phosphate and platelet-derived growth factor (PDGF) act via PDGF beta receptor-sphingosine 1-phosphate receptor complexes in airway smooth muscle cells
    Catherine Waters
    Department of Physiology and Pharmacology, Strathclyde Institute for Biomedical Sciences, University of Strathclyde, 27 Taylor St, Glasgow, G4 0NR, Scotland, United Kingdom
    J Biol Chem 278:6282-90. 2003
    ....
  34. ncbi request reprint Sphingosine kinase modulates microvascular tone and myogenic responses through activation of RhoA/Rho kinase
    Steffen Sebastian Bolz
    Institute of Physiology, Ludwig Maximilians University, Schillerstrasse 44, 80336 Muenchen, Germany
    Circulation 108:342-7. 2003
    ..RhoA and Rho kinase are important modulators of microvascular tone...
  35. ncbi request reprint Stem cell regulation by lysophospholipids
    Alice Pebay
    Centre for Neuroscience and Department of Pharmacology, The University of Melbourne, Parkville, Victoria 3010, Australia
    Prostaglandins Other Lipid Mediat 84:83-97. 2007
    ....
  36. ncbi request reprint Sphingosine kinase 1 (SK1) is recruited to nascent phagosomes in human macrophages: inhibition of SK1 translocation by Mycobacterium tuberculosis
    Christopher R Thompson
    Inflammation Program, University of Iowa Carver College of Medicine, Coralville, IA 52241, USA
    J Immunol 174:3551-61. 2005
    ..These data are consistent with a model in which M.tb inhibits both the activation and phagosomal translocation of SK1 to block the localized Ca2+ transients required for phagosome maturation...
  37. ncbi request reprint The sphingosine and diacylglycerol kinase superfamily of signaling kinases: localization as a key to signaling function
    Binks W Wattenberg
    Department of Medicine, University of Louisville, Louisville, KY, USA
    J Lipid Res 47:1128-39. 2006
    ..Therefore, changes in the membrane localization of these signaling kinases have profound effects not only on the production of signaling lipid phosphates but also on the metabolism of the upstream signaling lipids...
  38. ncbi request reprint Essential roles of sphingosine-1-phosphate and platelet-derived growth factor in the maintenance of human embryonic stem cells
    Alice Pebay
    Monash Institute of Medical Research, Laboratory of Embryonic Stem Cell Biology, Australian Stem Cell Centre, STRIP Monash University, Clayton VIC 3800, Australia
    Stem Cells 23:1541-8. 2005
    ..This study is the first demonstration of a role for lysophospholipid receptor signaling in the maintenance of stem cell pluri-potentiality...
  39. ncbi request reprint Enhancement of intracellular sphingosine-1-phosphate production by inositol 1,4,5-trisphosphate-evoked calcium mobilisation in HEK-293 cells: endogenous sphingosine-1-phosphate as a modulator of the calcium response
    Tomas Blom
    Department of Biology, Abo Akademi University, BioCity, Artillerigatan 6, 20520 Turku, Finland
    Cell Signal 17:827-36. 2005
    ..Importantly, 2-APB and U73122 inhibited the S1P-evoked production of intracellular S1P. S1P is therefore not likely a major calcium releasing second messenger in HEK-293 cells, but rather a secondary regulator of calcium mobilisation...