Sharad Kumar

Summary

Affiliation: South Australia
Country: Australia

Publications

  1. pmc A potential role for NEDD1 and the centrosome in senescence of mouse embryonic fibroblasts
    J A Manning
    Centre for Cancer Biology, SA Pathology, Adelaide, SA, Australia
    Cell Death Dis 1:e35. 2010
  2. pmc Respiratory distress and perinatal lethality in Nedd4-2-deficient mice
    Natasha A Boase
    Department of Haematology, Centre for Cancer Biology, SA Pathology, PO Box 14, Rundle Mall, Adelaide, South Australia 5000, Australia
    Nat Commun 2:287. 2011
  3. pmc A direct interaction with NEDD1 regulates gamma-tubulin recruitment to the centrosome
    Jantina A Manning
    Centre for Cancer Biology, SA Pathology, Adelaide, South Australia, Australia
    PLoS ONE 5:e9618. 2010
  4. doi request reprint Caspase 2 in apoptosis, the DNA damage response and tumour suppression: enigma no more?
    Sharad Kumar
    Centre for Cancer Biology, Department of Haematology, SA Pathology, Frome Road, Adelaide, SA5000, Australia
    Nat Rev Cancer 9:897-903. 2009
  5. pmc UTX coordinates steroid hormone-mediated autophagy and cell death
    Donna Denton
    1 Centre for Cancer Biology, SA Pathology, Frome Road, Adelaide, South Australia 5000, Australia 2 Division of Health Sciences, University of South Australia, Adelaide, South Australia 5001, Australia
    Nat Commun 4:2916. 2013
  6. pmc Autophagy, not apoptosis, is essential for midgut cell death in Drosophila
    Donna Denton
    Department of Haematology, Centre for Cancer Biology, Adelaide, SA, Australia
    Curr Biol 19:1741-6. 2009
  7. doi request reprint Ndfip1-deficient mice have impaired DMT1 regulation and iron homeostasis
    Natalie J Foot
    Department of Hematology, Centre for Cancer Biology, SA Pathology, Adelaide, Australia
    Blood 117:638-46. 2011
  8. doi request reprint Regulation of the divalent metal ion transporter DMT1 and iron homeostasis by a ubiquitin-dependent mechanism involving Ndfips and WWP2
    Natalie J Foot
    Department of Haematology, Hanson Institute, Adelaide, Australia
    Blood 112:4268-75. 2008
  9. ncbi request reprint Ecdysone-mediated up-regulation of the effector caspase DRICE is required for hormone-dependent apoptosis in Drosophila cells
    Zoé E Kilpatrick
    Hanson Institute, Institute of Medical and Veterinary Science, PO Box 14, Rundle Mall, Adelaide, SA 5000, Australia
    J Biol Chem 280:11981-6. 2005
  10. ncbi request reprint Regulation of neuronal voltage-gated sodium channels by the ubiquitin-protein ligases Nedd4 and Nedd4-2
    Andrew B Fotia
    Hanson Institute, Institute of Medical and Veterinary Science, Frome Road, Adelaide, South Australia 5000, Australia
    J Biol Chem 279:28930-5. 2004

Collaborators

  • David A Jans
  • Martin F Lavin
  • David J Adams
  • Michael D Garrick
  • Andreas Bergmann
  • Claire H Wilson
  • Gerry Melino
  • Lin Zhao
  • Christoph Korbmacher
  • Seong Seng Tan
  • Philippe Bouillet
  • Donna Denton
  • Dimitrios Cakouros
  • Kathryn Mills
  • Loretta Dorstyn
  • Andrew B Fotia
  • Natalie J Foot
  • Jantina A Manning
  • David I Cook
  • Devendra K Hiwase
  • Tasman J Daish
  • Hazel E Dalton
  • Eric H Baehrecke
  • Belinda C Baliga
  • May T Aung-Htut
  • Joseph Puccini
  • Linda M Shearwin-Whyatt
  • Sophie Wiszniak
  • Anne K Voss
  • Sonia Shalini
  • Natasha A Boase
  • J A Manning
  • Baoli Yang
  • Bhupendra Shravage
  • Rachel Simin
  • Lien Ha Ho
  • Anuwat Dinudom
  • Jantina Manning
  • Linda Shearwin-Whyatt
  • Paul A Colussi
  • Zoé E Kilpatrick
  • Tasman Daish
  • Magtouf Gatei
  • Wenying Zhu
  • Quenten Schwarz
  • Genevieve Secker
  • Natasha Harvey
  • Shannon Nicolson
  • Rachael Lumb
  • Michaela Scherer
  • Nirmal Lorensuhewa
  • Samuela Kabbara
  • Frank Koentgen
  • Kristen Ho
  • Loretta E Dorstyn
  • Devendra Hiwase
  • Eleanora Candi
  • Tatiana Tsoutsman
  • Yew Ann Leong
  • Grigori Y Rychkov
  • Scott L Townley
  • Chris Semsarian
  • Joanna M Risk
  • Catherine L Day
  • Deborah L Berry
  • Robyn Taylor
  • Junia Melo
  • Deborah L White
  • Verity Saunders
  • Stephanie Zrim
  • Phuong Dang
  • Laura Eadie
  • Simon A Koblar
  • Duncan Hewett
  • L Bik To
  • Timothy P Hughes
  • Amity Frede
  • Natalie Foot
  • Darren L Brown
  • Fiona G Wylie
  • Jenny Ekberg
  • Jennifer L Stow
  • Philip Poronnik
  • Keith E Shearwin
  • Manisha M Dias
  • Stuart H Read
  • Jan Peter Koch
  • Damali Martin

Detail Information

Publications36

  1. pmc A potential role for NEDD1 and the centrosome in senescence of mouse embryonic fibroblasts
    J A Manning
    Centre for Cancer Biology, SA Pathology, Adelaide, SA, Australia
    Cell Death Dis 1:e35. 2010
    ..These data show that a loss of centrosomal integrity may contribute to the entry of MEFs into senescence in culture, and that centrosomal disruption can cause senescence...
  2. pmc Respiratory distress and perinatal lethality in Nedd4-2-deficient mice
    Natasha A Boase
    Department of Haematology, Centre for Cancer Biology, SA Pathology, PO Box 14, Rundle Mall, Adelaide, South Australia 5000, Australia
    Nat Commun 2:287. 2011
    ..Thus, we provide critical in vivo evidence that Nedd4-2 is essential for correct regulation of ENaC expression, fetal and postnatal lung function and animal survival...
  3. pmc A direct interaction with NEDD1 regulates gamma-tubulin recruitment to the centrosome
    Jantina A Manning
    Centre for Cancer Biology, SA Pathology, Adelaide, South Australia, Australia
    PLoS ONE 5:e9618. 2010
    ..Hence, this study defines the binding site on NEDD1 that is required for its interaction with gamma-tubulin, and shows that this interaction is required for the correct localization of gamma-tubulin...
  4. doi request reprint Caspase 2 in apoptosis, the DNA damage response and tumour suppression: enigma no more?
    Sharad Kumar
    Centre for Cancer Biology, Department of Haematology, SA Pathology, Frome Road, Adelaide, SA5000, Australia
    Nat Rev Cancer 9:897-903. 2009
    ..These findings are unexpected and have important implications for our understanding of tumorigenesis and the treatment of cancer...
  5. pmc UTX coordinates steroid hormone-mediated autophagy and cell death
    Donna Denton
    1 Centre for Cancer Biology, SA Pathology, Frome Road, Adelaide, South Australia 5000, Australia 2 Division of Health Sciences, University of South Australia, Adelaide, South Australia 5001, Australia
    Nat Commun 4:2916. 2013
    ....
  6. pmc Autophagy, not apoptosis, is essential for midgut cell death in Drosophila
    Donna Denton
    Department of Haematology, Centre for Cancer Biology, Adelaide, SA, Australia
    Curr Biol 19:1741-6. 2009
    ....
  7. doi request reprint Ndfip1-deficient mice have impaired DMT1 regulation and iron homeostasis
    Natalie J Foot
    Department of Hematology, Centre for Cancer Biology, SA Pathology, Adelaide, Australia
    Blood 117:638-46. 2011
    ..These data demonstrate that Ndfip1 is a critical mediator of DMT1 regulation in vivo, particularly under iron restricted conditions...
  8. doi request reprint Regulation of the divalent metal ion transporter DMT1 and iron homeostasis by a ubiquitin-dependent mechanism involving Ndfips and WWP2
    Natalie J Foot
    Department of Haematology, Hanson Institute, Adelaide, Australia
    Blood 112:4268-75. 2008
    ..This novel mechanism of regulating iron homeostasis suggests that Ndfips and WWP2 may contribute to diseases involving aberrant iron transport...
  9. ncbi request reprint Ecdysone-mediated up-regulation of the effector caspase DRICE is required for hormone-dependent apoptosis in Drosophila cells
    Zoé E Kilpatrick
    Hanson Institute, Institute of Medical and Veterinary Science, PO Box 14, Rundle Mall, Adelaide, SA 5000, Australia
    J Biol Chem 280:11981-6. 2005
    ..Combined with previous observations, our results indicate that transcriptional regulation of the components of the core apoptotic machinery plays a key role in hormone-regulated programmed cell death during Drosophila development...
  10. ncbi request reprint Regulation of neuronal voltage-gated sodium channels by the ubiquitin-protein ligases Nedd4 and Nedd4-2
    Andrew B Fotia
    Hanson Institute, Institute of Medical and Veterinary Science, Frome Road, Adelaide, South Australia 5000, Australia
    J Biol Chem 279:28930-5. 2004
    ..Interestingly, Nedd4 suppressed the activity of Na(v)1.2 and Na(v)1.7 but was a poor inhibitor of Na(v)1.8. Our results provide evidence that Nedd4 and Nedd4-2 are likely to be key regulators of specific neuronal Na(v) channels in vivo...
  11. ncbi request reprint Drosophila caspase DRONC is required for specific developmental cell death pathways and stress-induced apoptosis
    Tasman J Daish
    Hanson Institute, Institute of Medical and Veterinary Science, PO Box 14, Rundle Mall, Adelaide, South Australia 5000, Australia
    Dev Cell 7:909-15. 2004
    ..Our results indicate that DRONC is involved in specific developmental cell death pathways and that in some tissues, effector caspase activation and cell death can occur independently of DRONC...
  12. doi request reprint Nedd1 expression as a marker of dynamic centrosomal localization during mouse embryonic development
    Jantina A Manning
    Hanson Institute, Institute of Medical and Veterinary Science, PO Box 14, Rundle Mall, Adelaide, SA, 5000, Australia
    Histochem Cell Biol 129:751-64. 2008
    ..This study reveals the localization of Nedd1 and the centrosome during important processes in mouse embryogenesis and provides a basis for further study into its role in development...
  13. ncbi request reprint The function of the Drosophila caspase DRONC in cell death and development
    Kathryn Mills
    Hanson Institute, Adelaide, Australia
    Cell Cycle 4:744-6. 2005
    ..Insights gained from the dronc mutants will help in advancing our understanding of caspase function in various developmental pathways...
  14. pmc Loss of caspase-2 augments lymphomagenesis and enhances genomic instability in Atm-deficient mice
    Joseph Puccini
    Department of Hematology, Centre for Cancer Biology, SA Pathology, Adelaide, SA 5000, Australia
    Proc Natl Acad Sci U S A 110:19920-5. 2013
    ....
  15. pmc Ecdysone receptor directly binds the promoter of the Drosophila caspase dronc, regulating its expression in specific tissues
    Dimitrios Cakouros
    Hanson Institute, Institute of Medical and Veterinary Science, Frome Rd, Adelaide, SA 5000, Australia
    J Cell Biol 165:631-40. 2004
    ..These results indicate that direct binding of EcR-Usp is crucial for controlling the timing of dronc expression in specific tissues...
  16. pmc Larval midgut destruction in Drosophila: not dependent on caspases but suppressed by the loss of autophagy
    Donna Denton
    Department of Haematology, Centre for Cancer Biology, SA Pathology, Adelaide, SA, Australia
    Autophagy 6:163-5. 2010
    ..These studies demonstrate that Drosophila midgut PCD is executed by a novel mechanism where caspases are apparently dispensable, but that requires autophagy...
  17. doi request reprint The ubiquitin ligase Nedd4 regulates craniofacial development by promoting cranial neural crest cell survival and stem-cell like properties
    Sophie Wiszniak
    Centre for Cancer Biology, SA Pathology, Frome Road, Adelaide, 5000, Australia
    Dev Biol 383:186-200. 2013
    ..Our analyses therefore uncover an essential role for Nedd4 in a subset of cranial NC cells and highlight E3 ubiquitin ligases as a likely point of convergence for multiple NC signalling pathways and transcription factor networks. ..
  18. doi request reprint Methods and protocols for studying cell death in Drosophila
    Donna Denton
    Hanson Institute, Institute of Medical and Veterinary Science, Adelaide, Australia
    Methods Enzymol 446:17-37. 2008
    ..In addition, one of the advantages of the use of Drosophila as model is the ability to examine genetic interactions with various components of the cell death pathway...
  19. pmc A tumor suppressor function for caspase-2
    Lien Ha Ho
    Centre for Cancer Biology, Hanson Institute, Frome Road, Adelaide, SA 5000, Australia
    Proc Natl Acad Sci U S A 106:5336-41. 2009
    ..These data show that loss of caspase-2 results in an increased ability of cells to acquire a transformed phenotype and become malignant, indicating that caspase-2 is a tumor suppressor protein...
  20. ncbi request reprint Regulation of functional diversity within the Nedd4 family by accessory and adaptor proteins
    Linda Shearwin-Whyatt
    Molecular Regulation Laboratory, Hanson Institute, IMVS, Adelaide, Australia
    Bioessays 28:617-28. 2006
    ....
  21. ncbi request reprint The ubiquitin-protein ligases Nedd4 and Nedd4-2 show similar ubiquitin-conjugating enzyme specificities
    Andrew B Fotia
    Hanson Institute, IMVS, Frome Road, P O Box 14, Rundle Mall, Adelaide, SA 5000, Australia
    Int J Biochem Cell Biol 38:472-9. 2006
    ..We also found that Ube2e3, an E2 previously shown to be used by Nedd4-2, is used less efficiently than UbcH5b. Our results suggest that for optimal ubiquitination Nedd4 and Nedd4-2 require the same E2 enzymes...
  22. ncbi request reprint Apoptosis. A cinderella caspase takes center stage
    Sharad Kumar
    Hanson Institute, Frome Road, Adelaide 5000, Australia
    Science 297:1290-1. 2002
  23. doi request reprint Analysing caspase activation and caspase activity in apoptotic cells
    Sharad Kumar
    Hanson Institute, Adelaide, Australia
    Methods Mol Biol 559:3-17. 2009
    ..This chapter outlines various commonly used assays for measuring caspase activity and detecting active caspases in cultured cells or tissue extracts...
  24. ncbi request reprint Transcriptional control of the core cell-death machinery
    Sharad Kumar
    Hanson Institute, IMVS, PO Box 14, Rundle Mall, Adelaide 5000, Australia
    Trends Biochem Sci 29:193-9. 2004
  25. doi request reprint Developmentally programmed cell death in Drosophila
    Donna Denton
    Centre for Cancer Biology, SA Pathology, Frome Road, Adelaide, SA 5000, Australia School of Molecular and Biomedical Science, University of Adelaide, North Terrace, Adelaide, SA 5005, Australia Division of Health Sciences, University of South Australia, Adelaide, SA 5001, Australia
    Biochim Biophys Acta 1833:3499-506. 2013
    ..This article is part of a Special Section entitled: Cell Death Pathways...
  26. ncbi request reprint N4WBP5A (Ndfip2), a Nedd4-interacting protein, localizes to multivesicular bodies and the Golgi, and has a potential role in protein trafficking
    Linda M Shearwin-Whyatt
    Hanson Institute, IMVS, Frome Road, Adelaide, SA 5000, Australia
    J Cell Sci 117:3679-89. 2004
    ..We propose that N4WBP5A acts as an adaptor to recruit Nedd4 family ubiquitin-protein ligases to the protein trafficking machinery...
  27. pmc Ecdysone-induced expression of the caspase DRONC during hormone-dependent programmed cell death in Drosophila is regulated by Broad-Complex
    Dimitrios Cakouros
    Hanson Centre for Cancer Research, Institute of Medical and Veterinary Science, Adelaide, SA 5000, Australia
    J Cell Biol 157:985-95. 2002
    ..Finally, we show that the dronc promoter has BR-C interaction sites, and that it can be transactivated by a specific isoform of BR-C. These results indicate that BR-C plays a key role in ecdysone-mediated caspase regulation...
  28. doi request reprint Caspase-2 protocols
    Loretta Dorstyn
    Centre for Cancer Biology, SA Pathology, Adelaide, Australia
    Methods Mol Biol 1133:71-87. 2014
    ..This chapter outlines commonly used methods to purify recombinant caspase-2 and assess its activity and function in vitro and in cultured cells or tissue extracts. ..
  29. ncbi request reprint NEDD1: function in microtubule nucleation, spindle assembly and beyond
    Jantina Manning
    Hanson Institute, Institute of Medical and Veterinary Science, PO Box 14, Rundle Mall, Adelaide, SA 5000, Australia
    Int J Biochem Cell Biol 39:7-11. 2007
    ..This short review summarises some of the recent work on NEDD1 and discusses how this interesting protein may have additional yet unexplored functions...
  30. ncbi request reprint Migrate, differentiate, proliferate, or die: pleiotropic functions of an apical "apoptotic caspase"
    Sharad Kumar
    Hanson Institute, Institute of Medical and Veterinary Science, P O Box 14, Rundle Mall, Adelaide, South Australia 5000, Australia
    Sci STKE 2004:pe49. 2004
    ..Recent work suggests that DRONC, the only initiator cell death caspase in Drosophila, may play a direct or indirect role in cell migration, sperm differentiation, and cell proliferation in addition to its function in cell death...
  31. ncbi request reprint Role of prodomain in importin-mediated nuclear localization and activation of caspase-2
    Belinda C Baliga
    Hanson Institute, Frome Road, Adelaide 5000, Australia
    J Biol Chem 278:4899-905. 2003
    ....
  32. ncbi request reprint The role of individual Nedd4-2 (KIAA0439) WW domains in binding and regulating epithelial sodium channels
    Andrew B Fotia
    Hanson Institute, Adelaide, SA, Australia
    FASEB J 17:70-2. 2003
    ..These data suggest that Nedd4-2 WW3 and WW4 interact with distinct, noninterchangeable sites in ENaC and that to prevent Na+ feedback control of ENaC it is necessary to occlude both sites...
  33. ncbi request reprint An arginine-histone methyltransferase, CARMER, coordinates ecdysone-mediated apoptosis in Drosophila cells
    Dimitrios Cakouros
    Hanson Institute, IMVS, Frome Road, Adelaide 5000, Australia
    J Biol Chem 279:18467-71. 2004
    ..Thus, the chromatin-modifying protein, CARMER, modulates cell death by controlling the hormone-dependent expression of the core cell death machinery...
  34. ncbi request reprint Role of Bcl-2 family of proteins in malignancy
    Belinda C Baliga
    Department of Haematology, Hanson Centre for Cancer Research, Institute of Medical and Veterinary Science, Frome Road, Adelaide, SA 5000, Australia
    Hematol Oncol 20:63-74. 2002
    ..This article summarizes what is currently known about Bcl-2-like proteins and how the evolving understanding of the biology of these proteins is paving way for the development of novel cancer therapeutics...
  35. ncbi request reprint Dasatinib cellular uptake and efflux in chronic myeloid leukemia cells: therapeutic implications
    Devendra K Hiwase
    Division of Haematology, Institute of Medical and Veterinary Science, University of Adelaide, Adelaide, South Australia, Australia
    Clin Cancer Res 14:3881-8. 2008
    ..The relevance of OCT-1 activity and efflux pumps in determining intracellular uptake and retention (IUR) of dasatinib was assessed...