Matthew J Watt

Summary

Affiliation: RMIT University
Country: Australia

Publications

  1. ncbi Beta-adrenergic stimulation of skeletal muscle HSL can be overridden by AMPK signaling
    Matthew J Watt
    Skeletal Muscle Research Laboratory, School of Medical Sciences, Royal Melbourne Institute of Technology, Bundoora, Victoria, Australia
    FASEB J 18:1445-6. 2004
  2. ncbi Hormone-sensitive lipase activity and triacylglycerol hydrolysis are decreased in rat soleus muscle by cyclopiazonic acid
    Matthew J Watt
    School of Medical Sciences, Royal Melbourne Institute of Technology, PO Box 71, Bundoora, Victoria 3083, Australia
    Am J Physiol Endocrinol Metab 285:E412-9. 2003
  3. pmc Effects of plasma adrenaline on hormone-sensitive lipase at rest and during moderate exercise in human skeletal muscle
    Matthew J Watt
    Department of Human Biology and Nutritional Sciences, University of Guelph, Guelph, Ontario N1G 2W1, Canada
    J Physiol 550:325-32. 2003
  4. ncbi Hormone-sensitive lipase activity and fatty acyl-CoA content in human skeletal muscle during prolonged exercise
    Matthew J Watt
    Department of Human Biology and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada
    J Appl Physiol (1985) 95:314-21. 2003
  5. ncbi Reduced plasma FFA availability increases net triacylglycerol degradation, but not GPAT or HSL activity, in human skeletal muscle
    Matthew J Watt
    Skeletal Muscle Research Laboratory, School of Medical Sciences, RMIT Univ, PO Box 71, Bundoora 3083, Victoria, Australia
    Am J Physiol Endocrinol Metab 287:E120-7. 2004
  6. ncbi Rapid upregulation of pyruvate dehydrogenase kinase activity in human skeletal muscle during prolonged exercise
    Matthew J Watt
    Department of Human Biology and Nutritional Sciences, University of Guelph, Guelph, Ontario N1G 2W1
    J Appl Physiol (1985) 97:1261-7. 2004
  7. ncbi Regulation of HSL serine phosphorylation in skeletal muscle and adipose tissue
    Matthew J Watt
    Cellular and Molecular Metabolism Laboratory, School of Medical Sciences, RMIT University, PO Box 71, Bundoora 3083, Victoria, Australia
    Am J Physiol Endocrinol Metab 290:E500-8. 2006
  8. ncbi Hormone-sensitive lipase is reduced in the adipose tissue of patients with type 2 diabetes mellitus: influence of IL-6 infusion
    M J Watt
    School of Medical Sciences, RMIT University, PO Box 71, Bundoora, 3083, Australia
    Diabetologia 48:105-12. 2005
  9. ncbi Suppression of plasma free fatty acids upregulates peroxisome proliferator-activated receptor (PPAR) alpha and delta and PPAR coactivator 1alpha in human skeletal muscle, but not lipid regulatory genes
    M J Watt
    Skeletal Muscle Research Laboratory, School of Medical Sciences, Royal Melbourne Institute of Technology, PO Box 27, Bundoora 3083, Victoria, Australia
    J Mol Endocrinol 33:533-44. 2004
  10. ncbi Adrenergic regulation of HSL serine phosphorylation and activity in human skeletal muscle during the onset of exercise
    Jason L Talanian
    Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada N1G 2W1
    Am J Physiol Regul Integr Comp Physiol 291:R1094-9. 2006

Detail Information

Publications74

  1. ncbi Beta-adrenergic stimulation of skeletal muscle HSL can be overridden by AMPK signaling
    Matthew J Watt
    Skeletal Muscle Research Laboratory, School of Medical Sciences, Royal Melbourne Institute of Technology, Bundoora, Victoria, Australia
    FASEB J 18:1445-6. 2004
    ..However, the increased IMTG degradation in LG suggests factors other than HSL activity are important for IMTG degradation...
  2. ncbi Hormone-sensitive lipase activity and triacylglycerol hydrolysis are decreased in rat soleus muscle by cyclopiazonic acid
    Matthew J Watt
    School of Medical Sciences, Royal Melbourne Institute of Technology, PO Box 71, Bundoora, Victoria 3083, Australia
    Am J Physiol Endocrinol Metab 285:E412-9. 2003
    ..It is possible that more powerful signals or a higher [Ca2+] may override the lipid-storage effect of the CPA-mediated effects during muscular contractions...
  3. pmc Effects of plasma adrenaline on hormone-sensitive lipase at rest and during moderate exercise in human skeletal muscle
    Matthew J Watt
    Department of Human Biology and Nutritional Sciences, University of Guelph, Guelph, Ontario N1G 2W1, Canada
    J Physiol 550:325-32. 2003
    ..The results also demonstrate that increased ERK 1/2 phosphorylation coincides with elevated HSL activity, indicating that ERK 1/2 may mediate the contraction-induced increase in HSL activity early in exercise...
  4. ncbi Hormone-sensitive lipase activity and fatty acyl-CoA content in human skeletal muscle during prolonged exercise
    Matthew J Watt
    Department of Human Biology and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada
    J Appl Physiol (1985) 95:314-21. 2003
    ..The accumulation of LCFA CoA in the 2nd h of exercise may also have reduced the flux through HSL and accounted for the reduction in IMTG utilization previously observed late in prolonged exercise...
  5. ncbi Reduced plasma FFA availability increases net triacylglycerol degradation, but not GPAT or HSL activity, in human skeletal muscle
    Matthew J Watt
    Skeletal Muscle Research Laboratory, School of Medical Sciences, RMIT Univ, PO Box 71, Bundoora 3083, Victoria, Australia
    Am J Physiol Endocrinol Metab 287:E120-7. 2004
    ..These changes in IMTG degradation appear to disassociate, however, from the activity of the key enzymes responsible for synthesis and degradation of this substrate...
  6. ncbi Rapid upregulation of pyruvate dehydrogenase kinase activity in human skeletal muscle during prolonged exercise
    Matthew J Watt
    Department of Human Biology and Nutritional Sciences, University of Guelph, Guelph, Ontario N1G 2W1
    J Appl Physiol (1985) 97:1261-7. 2004
    ..The increased PDK activity was independent of changes in intra-mitochondrial effectors, and PDK-2 and PDK-4 protein content, suggesting that it was caused by a change in the specific activity of the existing kinases...
  7. ncbi Regulation of HSL serine phosphorylation in skeletal muscle and adipose tissue
    Matthew J Watt
    Cellular and Molecular Metabolism Laboratory, School of Medical Sciences, RMIT University, PO Box 71, Bundoora 3083, Victoria, Australia
    Am J Physiol Endocrinol Metab 290:E500-8. 2006
    ..Taken together, these data indicate that HSL activity is maintained in the face of AMPK activation as a result of elevated HSL Ser(660) phosphorylation in adipose tissue but not skeletal muscle...
  8. ncbi Hormone-sensitive lipase is reduced in the adipose tissue of patients with type 2 diabetes mellitus: influence of IL-6 infusion
    M J Watt
    School of Medical Sciences, RMIT University, PO Box 71, Bundoora, 3083, Australia
    Diabetologia 48:105-12. 2005
    ..We assessed the adipose tissue hormone-sensitive lipase (HSL) mRNA expression, protein expression and HSL activity in patients with type 2 diabetes mellitus, and determined the effect of IL-6 administration on these measures...
  9. ncbi Suppression of plasma free fatty acids upregulates peroxisome proliferator-activated receptor (PPAR) alpha and delta and PPAR coactivator 1alpha in human skeletal muscle, but not lipid regulatory genes
    M J Watt
    Skeletal Muscle Research Laboratory, School of Medical Sciences, Royal Melbourne Institute of Technology, PO Box 27, Bundoora 3083, Victoria, Australia
    J Mol Endocrinol 33:533-44. 2004
    ..Despite these changes in transcription factors/coactivators, the mRNA content of lipid regulatory proteins was generally unaffected by plasma FFA availability...
  10. ncbi Adrenergic regulation of HSL serine phosphorylation and activity in human skeletal muscle during the onset of exercise
    Jason L Talanian
    Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada N1G 2W1
    Am J Physiol Regul Integr Comp Physiol 291:R1094-9. 2006
    ..This adrenergic stimulation contributes to the increase in HSL activity that occurs in human skeletal muscle in the first minute of exercise at 65% and 90% VO2 peak...
  11. doi Prolonged interleukin-6 administration enhances glucose tolerance and increases skeletal muscle PPARalpha and UCP2 expression in rats
    Anna G Holmes
    Diabetes and Metabolism Division, Cellular and Molecular Metabolism Laboratory, Baker Heart Research Institute, PO Box 6492, St Kilda Road Central, Melbourne, Victoria 8008, Australia
    J Endocrinol 198:367-74. 2008
    ..Our results do not support the notion that prolonged chronically elevated IL-6 impairs insulin action in vivo...
  12. ncbi Interleukin-6 increases insulin-stimulated glucose disposal in humans and glucose uptake and fatty acid oxidation in vitro via AMP-activated protein kinase
    Andrew L Carey
    Cellular and Molecular Metabolism Laboratory, School of Medical Sciences, Royal Melbourne Institute of Technology RMIT University, Australia
    Diabetes 55:2688-97. 2006
    ..Our results demonstrate that acute IL-6 treatment enhances insulin-stimulated glucose disposal in humans in vivo, while the effects of IL-6 on glucose and fatty acid metabolism in vitro appear to be mediated by AMPK...
  13. ncbi Chronic rosiglitazone treatment restores AMPKalpha2 activity in insulin-resistant rat skeletal muscle
    Sarah J Lessard
    School of Medical Sciences, RMIT University, Melbourne, Victoria, Australia
    Am J Physiol Endocrinol Metab 290:E251-7. 2006
    ..ACC phosphorylation was decreased with obesity (P < 0.05) but restored to the level of lean controls with RSG treatment. Our data demonstrate that RSG restores AMPK signaling in skeletal muscle of insulin-resistant obese Zucker rats...
  14. ncbi PGC-1alpha gene expression is down-regulated by Akt- mediated phosphorylation and nuclear exclusion of FoxO1 in insulin-stimulated skeletal muscle
    Robert J Southgate
    Cellular and Molecular Metabolism Laboratory, RMIT University, Bundoora, Victoria, Australia
    FASEB J 19:2072-4. 2005
    ..We conclude that insulin decreases the expression of genes involved in oxidative metabolism in healthy but not insulin resistant muscle, due to a decrease in FoxO1 phosphorylation and nuclear exclusion secondary to reduced Akt activity...
  15. ncbi Suppressing lipolysis increases interleukin-6 at rest and during prolonged moderate-intensity exercise in humans
    Anna G Holmes
    Skeletal Muscle Research Laboratory, School of Medical Sciences, RMIT University, P O Box 71, Bundoora 3083, Victoria, Australia
    J Appl Physiol (1985) 97:689-96. 2004
    ..98 pg/ml; NA, 19.23 +/- 2.50 pg/ml; P < 0.05) during exercise. In conclusion, these data demonstrate that circulating IL-6 is markedly elevated at rest and during prolonged moderate-intensity exercise when lipolysis is suppressed...
  16. doi High rates of muscle glycogen resynthesis after exhaustive exercise when carbohydrate is coingested with caffeine
    David J Pedersen
    School of Medical Sciences, RMIT University, Bundoora 3083, Victoria, Australia
    J Appl Physiol (1985) 105:7-13. 2008
    ..We provide the first evidence that in trained subjects coingestion of large amounts of Caff (8 mg/kg BM) with CHO has an additive effect on rates of postexercise muscle glycogen accumulation compared with consumption of CHO alone...
  17. ncbi Altering dietary nutrient intake that reduces glycogen content leads to phosphorylation of nuclear p38 MAP kinase in human skeletal muscle: association with IL-6 gene transcription during contraction
    M H Stanley Chan
    Skeletal Muscle Research Laboratory, School of Medical Sciences, Royal Melbourne Institute of Technology, Bundoora, Victoria, Australia
    FASEB J 18:1785-7. 2004
    ..Furthermore, phosphorylation of p38 MAPK in the nucleus appears to be an upstream target for IL-6, providing new insights into the regulation of IL-6 gene transcription...
  18. pmc HSP72 protects against obesity-induced insulin resistance
    Jason Chung
    Cellular and Molecular Metabolism Laboratory, Clinical Physiology, and Human Vascular Biology, Baker Heart Research Institute, Prahran, P O Box 6492, Victoria 8008, Australia
    Proc Natl Acad Sci U S A 105:1739-44. 2008
    ..These findings identify an essential role for HSP72 in blocking inflammation and preventing insulin resistance in the context of genetic obesity or high-fat feeding...
  19. pmc Fatty acids stimulate AMP-activated protein kinase and enhance fatty acid oxidation in L6 myotubes
    Matthew J Watt
    Cellular and Molecular Metabolism Laboratory, School of Medical Sciences, RMIT University, Victoria, Australia
    J Physiol 574:139-47. 2006
    ....
  20. ncbi Reduced glycogen availability is associated with increased AMPKalpha2 activity, nuclear AMPKalpha2 protein abundance, and GLUT4 mRNA expression in contracting human skeletal muscle
    Gregory R Steinberg
    Vincent s Institute and Department of Medicine, University of Melbourne, Fitzroy, Victoria, Australia
    Appl Physiol Nutr Metab 31:302-12. 2006
    ..We suggest that increased activation of AMPK alpha2 under conditions of low muscle glycogen enhances AMPK alpha2 nuclear translocation and increases GLUT4 mRNA expression in response to exercise in human skeletal muscle...
  21. pmc T-cell protein tyrosine phosphatase attenuates STAT3 and insulin signaling in the liver to regulate gluconeogenesis
    Atsushi Fukushima
    Department of Biochemistry and Molecular Biology, Monash University, Victoria, Australia
    Diabetes 59:1906-14. 2010
    ..Homozygous TCPTP-deficiency results in perinatal lethality prohibiting any informative assessment of TCPTP's role in glucose homeostasis. Here we have used Ptpn2+/- mice to investigate TCPTP's function in glucose homeostasis...
  22. ncbi Dysregulation of muscle lipid metabolism in rats selectively bred for low aerobic running capacity
    Fiona J Spargo
    Exercise Metabolism Group, School of Medical Sciences, Australia
    Am J Physiol Endocrinol Metab 292:E1631-6. 2007
    ..In conclusion, rats with low intrinsic aerobic capacity demonstrate abnormalities in lipid-handling capacity. These disruptions may, in part, be responsible for the increased risk of metabolic disorders observed in this phenotype...
  23. pmc Overexpression of carnitine palmitoyltransferase-1 in skeletal muscle is sufficient to enhance fatty acid oxidation and improve high-fat diet-induced insulin resistance
    Clinton R Bruce
    Cellular and Molecular Metabolism Laboratory, Baker IDI Heart and Diabetes Institute, Melbourne, Victoria, Australia
    Diabetes 58:550-8. 2009
    ....
  24. pmc Effects of dynamic exercise intensity on the activation of hormone-sensitive lipase in human skeletal muscle
    Matthew J Watt
    Department of Human Biology and Nutritional Sciences, University of Guelph, Guelph, Ontario N1G 2W1, Canada
    J Physiol 547:301-8. 2003
    ....
  25. ncbi Glucose ingestion blunts hormone-sensitive lipase activity in contracting human skeletal muscle
    Matthew J Watt
    Skeletal Muscle Research Laboratory, School of Medical Sciences, Royal Melbourne Institute of Technology University, PO Box 71, Bundoora 3083, Victoria, Australia
    Am J Physiol Endocrinol Metab 286:E144-50. 2004
    ..16 +/- 0.59, GLU, 1.37 +/- 0.37 mmol/min) in GLU compared with CON. These results demonstrate that circulating insulin and epinephrine play a role in HSLa in contracting skeletal muscle...
  26. pmc Carbohydrate ingestion reduces skeletal muscle acetylcarnitine availability but has no effect on substrate phosphorylation at the onset of exercise in man
    Matthew J Watt
    Department of Human Biology and Nutritional Sciences, University of Guelph, Ontario N1G 2W1, Canada
    J Physiol 544:949-56. 2002
    ..These data suggest that acetylcarnitine availability is unlikely to be the site of metabolic inertia during the transition from rest to steady-state moderate intensity exercise...
  27. doi Exercise training enhances white adipose tissue metabolism in rats selectively bred for low- or high-endurance running capacity
    Erin J Stephenson
    School of Medical Sciences, Royal Melbourne Institute of Technology, Bundoora, Australia
    Am J Physiol Endocrinol Metab 305:E429-38. 2013
    ..However, training does induce alterations in the activity and expression of several proteins that are essential to the intracellular regulation of WAT metabolism. ..
  28. ncbi Effects of reduced free fatty acid availability on hormone-sensitive lipase activity in human skeletal muscle during aerobic exercise
    Marcus O'Neill
    Department of Human Biology and Nutritional Sciences, University of Guelph, Guelph, ON, Canada N1G 2W1
    J Appl Physiol 97:1938-45. 2004
    ..In conclusion, reduced plasma FFA availability accompanied by increased epinephrine concentration did not further activate HSL beyond exercise alone...
  29. doi Phosphorylation of adipose triglyceride lipase Ser(404) is not related to 5'-AMPK activation during moderate-intensity exercise in humans
    Rachael R Mason
    Biology of Lipid Metabolism Laboratory, Department of Physiology, Monash University, Clayton, Victoria, Australia
    Am J Physiol Endocrinol Metab 303:E534-41. 2012
    ..Our results demonstrate that ATGL Ser(404) phosphorylation is not increased in mixed skeletal muscle during moderate-intensity exercise and that AMPK does not appear to be an activating kinase for ATGL Ser(404/406) in skeletal muscle...
  30. ncbi Decreased PDH activation and glycogenolysis during exercise following fat adaptation with carbohydrate restoration
    Trent Stellingwerff
    Department of Human Biology and Nutritional Sciences, University of Guelph, Ontario, Canada
    Am J Physiol Endocrinol Metab 290:E380-8. 2006
    ....
  31. doi Liver-specific suppressor of cytokine signaling-3 deletion in mice enhances hepatic insulin sensitivity and lipogenesis resulting in fatty liver and obesity
    Nirupa Sachithanandan
    St Vincent s Institute of Medical Research and Department of Medicine, University of Melbourne, Fitzroy, Victoria, Australia
    Hepatology 52:1632-42. 2010
    ..SOCS3 LKO mice fed an HFD also had elevated hypothalamic SOCS3 and fatty acid synthase expression and developed greater obesity due to increased food intake and reduced energy expenditure...
  32. pmc Intramuscular triacylglycerol, glycogen and acetyl group metabolism during 4 h of moderate exercise in man
    Matthew J Watt
    Department of Human Biology and Nutritional Sciences, University of Guelph, Ontario N1G 2W1
    J Physiol 541:969-78. 2002
    ..Although the factor underlying the PDHa decrease was not apparent, reduced pyruvate provision secondary to diminished glycolytic flux is the most likely mechanism...
  33. ncbi Ciliary neurotrophic factor prevents acute lipid-induced insulin resistance by attenuating ceramide accumulation and phosphorylation of c-Jun N-terminal kinase in peripheral tissues
    Matthew J Watt
    Cellular and Molecular Metabolism Laboratory, School of Medical Sciences, RMIT University, Bundoora, Victoria, Australia
    Endocrinology 147:2077-85. 2006
    ..These data support the use of CNTF as a potential therapeutic means to combat lipid-induced insulin resistance...
  34. ncbi CNTF reverses obesity-induced insulin resistance by activating skeletal muscle AMPK
    Matthew J Watt
    Cellular and Molecular Metabolism Laboratory, School of Medical Sciences, Royal Melbourne Institute of Technology, PO Box 71, Bundoora, 3083, Australia
    Nat Med 12:541-8. 2006
    ....
  35. pmc Ceramides contained in LDL are elevated in type 2 diabetes and promote inflammation and skeletal muscle insulin resistance
    James Boon
    Biology of Lipid Metabolism Laboratory, Department of Physiology, Monash University, Clayton, Victoria, Australia
    Diabetes 62:401-10. 2013
    ..These newly identified roles of LDL-ceramide suggest that strategies aimed at reducing hepatic ceramide production or reducing ceramide packaging into lipoproteins may improve skeletal muscle insulin action...
  36. pmc Pigment epithelium-derived factor regulates lipid metabolism via adipose triglyceride lipase
    Melissa L Borg
    Biology of Lipid Metabolism Laboratory, Department of Physiology, Monash University, Clayton, Victoria, Australia
    Diabetes 60:1458-66. 2011
    ..We used ATGL(-/-) mice to determine the role of PEDF in regulating lipid and glucose metabolism...
  37. ncbi Effects of hyperoxia on skeletal muscle carbohydrate metabolism during transient and steady-state exercise
    Trent Stellingwerff
    Dept of Human Biology and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada N1G 2W1
    J Appl Physiol (1985) 98:250-6. 2005
    ..The mechanism responsible for the decreased muscle glycogenolysis during hyperoxia in the present study is not clear...
  38. ncbi Cytokine gene expression in human skeletal muscle during concentric contraction: evidence that IL-8, like IL-6, is influenced by glycogen availability
    M H Stanley Chan
    Skeletal Muscle Research Laboratory, School of Medical Sciences, RMIT University, Bundoora, Victoria 3083, Australia
    Am J Physiol Regul Integr Comp Physiol 287:R322-7. 2004
    ..Furthermore, the mRNA abundance of IL-6 and IL-8 appears to be influenced by glycogen availability in the contracting muscle...
  39. ncbi Effects of reduced free fatty acid availability on skeletal muscle PDH activation during aerobic exercise. Pyruvate dehydrogenase
    Trent Stellingwerff
    Department of Human Biology and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada
    Am J Physiol Endocrinol Metab 284:E589-96. 2003
    ..The increased activation of PDH was not explained by changes in muscle pyruvate or the ATP/ADP ratio but may be related to a decrease in the NADH/NAD(+) ratio or an epinephrine-induced increase in calcium concentration...
  40. ncbi Regulation and role of hormone-sensitive lipase activity in human skeletal muscle
    Matthew J Watt
    Department of Human Biology and Nutritional Sciences, University of Guelph, Ontario, Canada
    Proc Nutr Soc 63:315-22. 2004
    ..For example, it must be remembered that the actual flux (IMTG degradation) through HSL may be allosterically inhibited during prolonged exercise as a result of the accumulation of long-chain fatty acyl-CoA...
  41. ncbi Pyruvate dehydrogenase activation and kinase expression in human skeletal muscle during fasting
    Lawrence L Spriet
    Department of Human Biology and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada N1G 2W1
    J Appl Physiol (1985) 96:2082-7. 2004
    ..The changes in PDK-4 expression and PDH activity did not coincide with increases in the transcriptional activators PPAR-alpha and FKHR...
  42. ncbi Adipose triacylglycerol lipase deletion alters whole body energy metabolism and impairs exercise performance in mice
    Elisabeth Huijsman
    Biology of Lipid Metabolism Laboratory, Department of Physiology, Monash University, Clayton, Victoria, Australia
    Am J Physiol Endocrinol Metab 297:E505-13. 2009
    ..These results demonstrate an essential role for both ATGL and HSL in maintaining adequate FFA supply to sustain normal substrate metabolism at rest and during exercise...
  43. doi Triglyceride lipases alter fuel metabolism and mitochondrial gene expression
    Matthew J Watt
    Department of Physiology, Monash University, Clayton, Victoria, Australia
    Appl Physiol Nutr Metab 34:340-7. 2009
    ..This review outlines the current understanding of the cellular regulation of TG lipases, lipolytic regulation, and the functional implications of manipulating ATGL and HSL in vivo...
  44. pmc Activation of PPARĪ± ameliorates hepatic insulin resistance and steatosis in high fructose-fed mice despite increased endoplasmic reticulum stress
    Stanley M H Chan
    Molecular Pharmacology for Diabetes Group, Health Innovations Research Institute and School of Health Sciences, RMIT University, Melbourne, Victoria, Australia
    Diabetes 62:2095-105. 2013
    ..Therefore, by reducing the accumulation of deleterious lipids, activation of PPARĪ± can ameliorate hepatic insulin resistance against increased ER stress...
  45. pmc Consumption of a high-fat diet, but not regular endurance exercise training, regulates hypothalamic lipid accumulation in mice
    Melissa L Borg
    Biology of Lipid Metabolism Laboratory, Department of Physiology, Monash University, Clayton, Victoria, 3800, Australia
    J Physiol 590:4377-89. 2012
    ..Overall, hypothalamic lipid accumulation is regulated by dietary lipid content and is refractory to change with endurance exercise training...
  46. doi Lipid metabolism in skeletal muscle: generation of adaptive and maladaptive intracellular signals for cellular function
    Matthew J Watt
    Biology of Lipid Metabolism Laboratory, Department of Physiology, Monash University, Clayton, Victoria 3800, Australia
    Am J Physiol Endocrinol Metab 302:E1315-28. 2012
    ....
  47. ncbi Adipocyte triglyceride lipase expression in human obesity
    Gregory R Steinberg
    St Vincent s Institute, Department of Medicine, The University of Melbourne, Fitzroy, Victoria, Australia
    Am J Physiol Endocrinol Metab 293:E958-64. 2007
    ....
  48. pmc Hematopoietic cell-restricted deletion of CD36 reduces high-fat diet-induced macrophage infiltration and improves insulin signaling in adipose tissue
    Hayley T Nicholls
    Cellular and Molecular Metabolism Laboratory, Baker IDI Heart and Diabetes Institute, Melbourne, Australia
    Diabetes 60:1100-10. 2011
    ..Accordingly, we hypothesized that it plays a role in saturated fatty acid-induced macrophage lipid accumulation and proinflammatory activation...
  49. ncbi Intramuscular triacylglycerol utilization in human skeletal muscle during exercise: is there a controversy?
    Matthew J Watt
    Department of Human Biology and Nutritional Sciences, University of Guelph, Guelph, Ontario N1G 2W1, Canada
    J Appl Physiol (1985) 93:1185-95. 2002
    ..Therefore, it is our contention that the muscle biopsy, isotope tracer, and (1)H-MRS techniques report significant and energetically important oxidation of free fatty acids derived from IMTGs during prolonged moderate exercise...
  50. ncbi Rosiglitazone enhances glucose tolerance by mechanisms other than reduction of fatty acid accumulation within skeletal muscle
    Sarah J Lessard
    Exercise Metabolism Group, RMIT University School of Medical Sciences, Bundoora, Victoria, Australia
    Endocrinology 145:5665-70. 2004
    ..Our data suggest, therefore, that rosiglitazone enhances glucose tolerance by mechanisms other than reduction of fatty acid accumulation within skeletal muscle...
  51. ncbi High-fat-fed obese glutathione peroxidase 1-deficient mice exhibit defective insulin secretion but protection from hepatic steatosis and liver damage
    Troy L Merry
    1 Department of Biochemistry and Molecular Biology, Monash University, Victoria, Australia
    Antioxid Redox Signal 20:2114-29. 2014
    ..Glutathione peroxidase 1 (Gpx1) is an antioxidant enzyme that eliminates H2O2. Here, we have used Gpx1(-/-) mice to assess the impact of oxidative stress on glucose homeostasis in the context of obesity...
  52. pmc Activating HSP72 in rodent skeletal muscle increases mitochondrial number and oxidative capacity and decreases insulin resistance
    Darren C Henstridge
    Cellular and Molecular Metabolism Laboratory, Baker IDI Heart and Diabetes Institute, Melbourne, Victoria, Australia
    Diabetes 63:1881-94. 2014
    ..Thus, the increased oxidative metabolism associated with activation of HSP72 has potential clinical implications not only for type 2 diabetes but also for other disorders where mitochondrial function is compromised...
  53. doi Fluxing the mitochondria to insulin resistance
    Matthew J Watt
    St Vincent s Institute of Medical Research, Fitzroy, Victoria, Australia
    Cell Metab 7:5-6. 2008
    ....
  54. doi Storing up trouble: does accumulation of intramyocellular triglyceride protect skeletal muscle from insulin resistance?
    Matthew J Watt
    Department of Physiology, Monash University, Melbourne, Victoria, Australia
    Clin Exp Pharmacol Physiol 36:5-11. 2009
    ..Whether lipase inhibitors are viable therapeutics to prevent obesity induced insulin resistance is unknown, but future studies examining tissue-specific ATGL/hormone-sensitive lipase knockouts will hopefully resolve this question...
  55. ncbi Cytokine Regulation of AMPK signalling
    Gregory R Steinberg
    St Vincent s Institute of Medical Research and Department of Medicine, University of Melbourne, Melbourne, Victoria, Australia
    Front Biosci (Landmark Ed) 14:1902-16. 2009
    ....
  56. ncbi Regulatory mechanisms in the interaction between carbohydrate and lipid oxidation during exercise
    L L Spriet
    Department of Human Biology and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada
    Acta Physiol Scand 178:443-52. 2003
    ..This review summarizes the work describing the interaction between CHO and fat metabolism in human skeletal muscle during exercise and presents the theories that may account for CHO/fat interaction during exercise...
  57. ncbi Ionomycin, but not physiologic doses of epinephrine, stimulates skeletal muscle interleukin-6 mRNA expression and protein release
    A G Holmes
    Skeletal Muscle Research Laboratory, The School of Medical Sciences, RMIT University, Bundoora 3038, Victoria, Australia
    Metabolism 53:1492-5. 2004
    ..In addition, ionomycin can stimulate IL-6 gene expression and protein release after only 1 hour of exposure...
  58. doi Brain-derived neurotrophic factor is produced by skeletal muscle cells in response to contraction and enhances fat oxidation via activation of AMP-activated protein kinase
    V B Matthews
    Cellular and Molecular Metabolism Laboratory, Diabetes and Metabolism Division, Baker Heart Research Institute, St Kilda Road Central, Melbourne, Victoria 8008, Australia
    Diabetologia 52:1409-18. 2009
    ..Brain-derived neurotrophic factor (BDNF) is produced in skeletal muscle, but its functional significance is unknown. We aimed to determine the signalling processes and metabolic actions of BDNF...
  59. ncbi Augmented expression and secretion of adipose-derived pigment epithelium-derived factor does not alter local angiogenesis or contribute to the development of systemic metabolic derangements
    Thomas V Lakeland
    Biology of Lipid Metabolism Laboratory, Department of Physiology, Monash University, Clayton, Victoria, Australia
    Am J Physiol Endocrinol Metab 306:E1367-77. 2014
    ..Together, these data indicate that adipocyte-generated PEDF enhances lipolysis but question the role of PEDF as a major antiangiogenic or proinflammatory mediator in adipose tissue in vivo. ..
  60. pmc Distinct roles of specific fatty acids in cellular processes: implications for interpreting and reporting experiments
    Matthew J Watt
    Dept of Physiology, Monash University, Clayton, VIC 3800, Australia
    Am J Physiol Endocrinol Metab 302:E1-3. 2012
    ..Hence, authors should avoid conclusions that generalize about "FAs" or "saturated FAs" or "high-fat diet" effects if only a single FA was used in the reported experiments...
  61. ncbi Stearoyl CoA desaturase 1 is elevated in obesity but protects against fatty acid-induced skeletal muscle insulin resistance in vitro
    S K Pinnamaneni
    Cellular and Molecular Metabolism Laboratory, School of Medical Sciences, RMIT University, Melbourne, Victoria, Australia
    Diabetologia 49:3027-37. 2006
    ..The aim of the present study was to assess the molecular mechanisms that implicate SCD1 in the aetiology of fatty acid-induced insulin resistance...
  62. ncbi Increased lipid accumulation and insulin resistance in transgenic mice expressing DGAT2 in glycolytic (type II) muscle
    Malin C Levin
    Gladstone Institute of Cardiovascular Disease, 1650 Owens St, San Francisco, CA 94158, USA
    Am J Physiol Endocrinol Metab 293:E1772-81. 2007
    ..We conclude that DGAT2-mediated lipid deposition specifically in glycolytic muscle promotes insulin resistance in this tissue and may contribute to the development of diabetes...
  63. ncbi Effect of epinephrine on glucose disposal during exercise in humans: role of muscle glycogen
    Matthew J Watt
    Exercise, Muscle and Metabolism Unit, School of Health Sciences, Deakin University, Burwood, Victoria 3125, Australia
    Am J Physiol Endocrinol Metab 283:E578-83. 2002
    ....
  64. ncbi Regulation of glucose kinetics during intense exercise in humans: effects of alpha- and beta-adrenergic blockade
    Kirsten F Howlett
    Exercise, Muscle and Metabolism Unit, School of Health Sciences, Deakin University, Burwood, Australia
    Metabolism 52:1615-20. 2003
    ..05) in BLK. These findings demonstrate that adrenergic blockade during intense exercise results in a blunted plasma glucose response that is due to enhanced glucose uptake, with no significant change in glucose production...
  65. ncbi Saturated, but not n-6 polyunsaturated, fatty acids induce insulin resistance: role of intramuscular accumulation of lipid metabolites
    Jong Sam Lee
    Department of Physical Therapy, College of Medicine, Eulji University, Young Du Dong, Jung Gu, Daejeon, Seoul, South Korea
    J Appl Physiol (1985) 100:1467-74. 2006
    ..Diets rich in n-6 PUFA appear to prevent insulin resistance by directing fat into TAG, rather than other lipid metabolites...
  66. ncbi Ciliary neurotrophic factor suppresses hypothalamic AMP-kinase signaling in leptin-resistant obese mice
    Gregory R Steinberg
    St Vincent s Institute, 9 Princes Street, Fitzroy, Victoria 3065, Australia
    Endocrinology 147:3906-14. 2006
    ..A HFD blunted leptin but not CNTF effects on AMPK signaling in the ARC and PVN. In summary, these data demonstrate that CNTF(Ax15) bypasses diet-induced leptin resistance to reduce hypothalamic AMPK activity...
  67. doi Metabolic remodeling in adipocytes promotes ciliary neurotrophic factor-mediated fat loss in obesity
    Seamus Crowe
    St Vincent s Institute of Medical Research and the Department of Medicine, The University of Melbourne, Fitzroy, Victoria 3065, Australia
    Endocrinology 149:2546-56. 2008
    ..We conclude that CNTF reprograms adipose tissue to promote mitochondrial biogenesis, enhancing oxidative capacity and reducing lipogenic capacity, thereby resulting in triglyceride loss...
  68. ncbi Thiazolidinediones enhance skeletal muscle triacylglycerol synthesis while protecting against fatty acid-induced inflammation and insulin resistance
    Mark K Todd
    Department of Kinesiology, University of Southern California, Los Angeles, California, USA
    Am J Physiol Endocrinol Metab 292:E485-93. 2007
    ..Despite this, TZDs suppress the inflammatory response to dietary lipid overload, and it is this mechanism that correlates strongly with insulin sensitivity...
  69. doi Adipose triglyceride lipase regulation of skeletal muscle lipid metabolism and insulin responsiveness
    Matthew J Watt
    St Vincent s Institute of Medical Research, Department of Medicine, University of Melbourne, Fitzroy, Victoria 3065, Australia
    Mol Endocrinol 22:1200-12. 2008
    ....
  70. ncbi Apoptosis in skeletal muscle myotubes is induced by ceramides and is positively related to insulin resistance
    Sarah M Turpin
    St Vincent s Institute of Medical Research, Fitzroy, Victoria 3064, Australia
    Am J Physiol Endocrinol Metab 291:E1341-50. 2006
    ..We conclude that lipoapoptosis occurs in skeletal muscle myotubes, at least partially via de novo ceramide accumulation, and that inhibiting downstream apoptotic signaling improves glucose uptake in vitro...
  71. ncbi Dissociation of hepatic steatosis and insulin resistance in mice overexpressing DGAT in the liver
    Mara Monetti
    Gladstone Institute of Cardiovascular Disease, 1650 Owens Street, San Francisco, CA 94158, USA
    Cell Metab 6:69-78. 2007
    ..Our results indicate that DGAT-mediated lipid accumulation in the liver is insufficient to cause insulin resistance and show that hepatic steatosis can occur independently of insulin resistance...
  72. ncbi Tumor necrosis factor alpha-induced skeletal muscle insulin resistance involves suppression of AMP-kinase signaling
    Gregory R Steinberg
    St Vincent s Institute and Department of Medicine, University of Melbourne, Fitzroy, Vic, 3065, Australia
    Cell Metab 4:465-74. 2006
    ..Our data demonstrate that AMPK is an important TNFalpha signaling target and is a contributing factor to the suppression of fatty-acid oxidation and the development of lipid-induced insulin resistance in obesity...
  73. pmc Macrophage PPAR gamma is required for normal skeletal muscle and hepatic insulin sensitivity and full antidiabetic effects of thiazolidinediones
    Andrea L Hevener
    Department of Medicine, Division of Endocrinology and Metabolism, UCSD, La Jolla, California, USA
    J Clin Invest 117:1658-69. 2007
    ..These findings reveal an essential role of PPAR gamma in macrophages for the maintenance of whole-body insulin action and in mediating the antidiabetic actions of TZDs...
  74. ncbi Regulation and function of triacylglycerol lipases in cellular metabolism
    Matthew J Watt
    Department of Physiology, Monash University, Clayton, VIC 3800, Australia
    Biochem J 414:313-25. 2008
    ..In the present review, we discuss the metabolic regulation and function of TAG lipases with a focus on HSL (hormone-sensitive lipase), ATGL (adipose triacylglycerol lipase) and newly identified members of the lipolytic proteome...