Wilhelmina M Huston

Summary

Affiliation: Queensland University of Technology
Country: Australia

Publications

  1. doi request reprint The IL-6 response to Chlamydia from primary reproductive epithelial cells is highly variable and may be involved in differential susceptibility to the immunopathological consequences of chlamydial infection
    Kelly Cunningham
    Institute of Health and Biomedical Innovation, Queensland University of Technology, Q Block, 60 Musk Ave, Kelvin Grove, QLD 4059, Australia
    BMC Immunol 14:50. 2013
  2. doi request reprint HtrA, RseP, and Tsp proteins do not elicit a pathology-related serum IgG response during sexually transmitted infection with Chlamydia trachomatis
    Wilhelmina M Huston
    Institute of Health and Biomedical Innovation and School of Life Sciences, Queensland University of Technology, Kelvin Grove, Qld 4053, Australia
    J Reprod Immunol 85:168-71. 2010
  3. pmc Unique residues involved in activation of the multitasking protease/chaperone HtrA from Chlamydia trachomatis
    Wilhelmina M Huston
    Institute of Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove, Queensland, Australia
    PLoS ONE 6:e24547. 2011
  4. doi request reprint Vaccination to protect against infection of the female reproductive tract
    Wilhelmina M Huston
    Institute of Health and Biomedical Innovation, 60 Musk Avenue, Queensland University of Technology, Kelvin Grove, Queensland, Australia
    Expert Rev Clin Immunol 8:81-94. 2012
  5. doi request reprint Bacterial proteases from the intracellular vacuole niche; protease conservation and adaptation for pathogenic advantage
    Wilhelmina M Huston
    Institute of Health and Biomedical Innovation and School of Life Sciences, Queensland University of Technology, Brisbane, QLD, Australia
    FEMS Immunol Med Microbiol 59:1-10. 2010
  6. pmc Chlamydia trachomatis responds to heat shock, penicillin induced persistence, and IFN-gamma persistence by altering levels of the extracytoplasmic stress response protease HtrA
    Wilhelmina M Huston
    Institute of Health and Biomedical Innovation, 60 Musk Ave, Queensland University of Technology, Kelvin Grove, QLD, 4059, Australia
    BMC Microbiol 8:190. 2008
  7. doi request reprint Apoptosis is induced in Chlamydia trachomatis-infected HEp-2 cells by the addition of a combination innate immune activation compounds and the inhibitor wedelolactone
    Wilhelmina M Huston
    Institute of Health and Biomedical Innovation and School of Life Sciences, Queensland University of Technology, Kelvin Grove, QLD, Australia
    Am J Reprod Immunol 65:460-5. 2011
  8. ncbi request reprint Expression and characterisation of a major c-type cytochrome encoded by gene kustc0563 from Kuenenia stuttgartiensis as a recombinant protein in Escherichia coli
    Wilhelmina M Huston
    Department of Biology Area 10, University of York, Heslington, York YO10 5YW, UK
    Protein Expr Purif 51:28-33. 2007
  9. doi request reprint the active site residue V266 of Chlamydial HtrA is critical for substrate binding during both in vitro and in vivo conditions
    Sarina Gloeckl
    Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, QLD, Australia
    J Mol Microbiol Biotechnol 22:10-6. 2012
  10. ncbi request reprint The temperature activated HtrA protease from pathogen Chlamydia trachomatis acts as both a chaperone and protease at 37 degrees C
    Wilhelmina M Huston
    Institute of Health and Biomedical Innovation and School of Life Sciences, Faculty of Science, Queensland University of Technology, Brisbane, Australia
    FEBS Lett 581:3382-6. 2007

Collaborators

Detail Information

Publications20

  1. doi request reprint The IL-6 response to Chlamydia from primary reproductive epithelial cells is highly variable and may be involved in differential susceptibility to the immunopathological consequences of chlamydial infection
    Kelly Cunningham
    Institute of Health and Biomedical Innovation, Queensland University of Technology, Q Block, 60 Musk Ave, Kelvin Grove, QLD 4059, Australia
    BMC Immunol 14:50. 2013
    ..This study aimed to investigate the role of primary human cellular responses to chlamydial stress response proteases and chlamydial infection to further identify the immune processes involved in serious disease sequelae...
  2. doi request reprint HtrA, RseP, and Tsp proteins do not elicit a pathology-related serum IgG response during sexually transmitted infection with Chlamydia trachomatis
    Wilhelmina M Huston
    Institute of Health and Biomedical Innovation and School of Life Sciences, Queensland University of Technology, Kelvin Grove, Qld 4053, Australia
    J Reprod Immunol 85:168-71. 2010
    ..Serum IgG(1-4) antibody subclasses reactive with these antigens was not significantly different between cohorts, although different responses to each antigen were detected...
  3. pmc Unique residues involved in activation of the multitasking protease/chaperone HtrA from Chlamydia trachomatis
    Wilhelmina M Huston
    Institute of Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove, Queensland, Australia
    PLoS ONE 6:e24547. 2011
    ..Therefore, we have revealed novel residues involved in the activation of CtHtrA which are likely to have important in vivo implications for outer membrane protein assembly...
  4. doi request reprint Vaccination to protect against infection of the female reproductive tract
    Wilhelmina M Huston
    Institute of Health and Biomedical Innovation, 60 Musk Avenue, Queensland University of Technology, Kelvin Grove, Queensland, Australia
    Expert Rev Clin Immunol 8:81-94. 2012
    ..Despite these difficulties, there are numerous ongoing efforts to develop effective vaccines against these infectious agents and it is likely that this important human health field will see further major developments in the next 5 years...
  5. doi request reprint Bacterial proteases from the intracellular vacuole niche; protease conservation and adaptation for pathogenic advantage
    Wilhelmina M Huston
    Institute of Health and Biomedical Innovation and School of Life Sciences, Queensland University of Technology, Brisbane, QLD, Australia
    FEMS Immunol Med Microbiol 59:1-10. 2010
    ..This review summarizes the recent findings of novel protease functions from the intracellular human pathogenic bacteria that reside exclusively in vacuoles...
  6. pmc Chlamydia trachomatis responds to heat shock, penicillin induced persistence, and IFN-gamma persistence by altering levels of the extracytoplasmic stress response protease HtrA
    Wilhelmina M Huston
    Institute of Health and Biomedical Innovation, 60 Musk Ave, Queensland University of Technology, Kelvin Grove, QLD, 4059, Australia
    BMC Microbiol 8:190. 2008
    ..Recombinant purified C. trachomatis HtrA has been previously shown to have both activities. This investigation examined the physiological role of Chlamydia trachomatis HtrA...
  7. doi request reprint Apoptosis is induced in Chlamydia trachomatis-infected HEp-2 cells by the addition of a combination innate immune activation compounds and the inhibitor wedelolactone
    Wilhelmina M Huston
    Institute of Health and Biomedical Innovation and School of Life Sciences, Queensland University of Technology, Kelvin Grove, QLD, Australia
    Am J Reprod Immunol 65:460-5. 2011
    ..Innate immune activation of human cells, for some intracellular pathogens, is advantageous for vacuole morphology and pathogenic viability. It is unknown whether innate immune activation is advantageous to Chlamydia trachomatis viability...
  8. ncbi request reprint Expression and characterisation of a major c-type cytochrome encoded by gene kustc0563 from Kuenenia stuttgartiensis as a recombinant protein in Escherichia coli
    Wilhelmina M Huston
    Department of Biology Area 10, University of York, Heslington, York YO10 5YW, UK
    Protein Expr Purif 51:28-33. 2007
    ..The predicted protein sequence for the cytochrome suggests it to be a predominantly alpha-helical protein, and this is supported by circular dichroism...
  9. doi request reprint the active site residue V266 of Chlamydial HtrA is critical for substrate binding during both in vitro and in vivo conditions
    Sarina Gloeckl
    Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, QLD, Australia
    J Mol Microbiol Biotechnol 22:10-6. 2012
    ..Notably, however, the interaction was significantly altered in 35 out of 69 clones when residue V266 was mutated, indicating that this residue has an important function during substrate binding...
  10. ncbi request reprint The temperature activated HtrA protease from pathogen Chlamydia trachomatis acts as both a chaperone and protease at 37 degrees C
    Wilhelmina M Huston
    Institute of Health and Biomedical Innovation and School of Life Sciences, Faculty of Science, Queensland University of Technology, Brisbane, Australia
    FEBS Lett 581:3382-6. 2007
    ..trachomatis HtrA functions as both a chaperone and protease at 37 degrees C. These properties are consistent with the developmental cycle of this obligate intracellular bacterium...
  11. doi request reprint Identification of a serine protease inhibitor which causes inclusion vacuole reduction and is lethal to Chlamydia trachomatis
    Sarina Gloeckl
    Institute of Health and Biomedical Innovation and School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, Kelvin Grove, QLD, 4059, Australia
    Mol Microbiol 89:676-89. 2013
    ....
  12. doi request reprint Characterization of in vitro Chlamydia muridarum persistence and utilization in an in vivo mouse model of Chlamydia vaccine
    Alison J Carey
    Institute of Health and Biomedical Innovation, School of Biomedical Science, Faculty of Health, Queensland University of Technology, Brisbane, QLD, Australia
    Am J Reprod Immunol 69:475-85. 2013
    ..In vitro chlamydial persistence has been well characterized in various strains; however, there have been no reported studies in C. muridarum...
  13. doi request reprint Delivery of nitric oxide for analysis of the function of cytochrome c'
    Lindsay J Cole
    Department of Biology, University of York, Heslington, York, United Kingdom
    Methods Enzymol 436:21-33. 2008
    ..We describe a number of techniques for steady-state UV/Vis spectrophotometry and pre-steady-state stopped-flow spectrophotometry analysis of cytochrome c'...
  14. pmc The protease inhibitor JO146 demonstrates a critical role for CtHtrA for Chlamydia trachomatis reversion from penicillin persistence
    Vanissa A Ong
    School of Biomedical Sciences, Institute of Health and Biomedical Innovation, Queensland University of Technology Brisbane, QLD, Australia
    Front Cell Infect Microbiol 3:100. 2013
    ..Thus, CtHtrA is an essential protease with both replicative phase and stress condition functions for Chlamydia trachomatis. ..
  15. ncbi request reprint Chlamydial infection of immune cells: altered function and implications for disease
    Kenneth W Beagley
    Institute of Health and Biomedical Innovation, Queensland University of Technology, Australia
    Crit Rev Immunol 29:275-305. 2009
    ..In this paper, we review the evidence for the growth of Chlamydia in immune cells, particularly monocytes/macrophages and dendritic cells, and describe how infection may affect the function of these cells...
  16. ncbi request reprint Heterologous overexpression and purification of cytochrome c' from Rhodobacter capsulatus and a mutant (K42E) in the dimerization region. Mutation does not alter oligomerization but impacts the heme iron spin state and nitric oxide binding properties
    Wilhelmina M Huston
    Department of Biology Area 10, University of York, Heslington, York, YO10 5YW, United Kingdom
    Biochemistry 45:4388-95. 2006
    ....
  17. doi request reprint Cytochrome P450 isozyme protein verified in the skin of southern hemisphere humpback whales (Megaptera novaeangliae): implications for biochemical biomarker assessment
    Courtney A Waugh
    The University of Queensland, The National Research Centre for Environmental Toxicology, Brisbane, QLD 4108, Australia
    Mar Pollut Bull 62:758-61. 2011
    ..The detection of CYP1A1 in the integument of the humpback whale affords the opportunity for further quantitative non-destructive investigations of enzyme activity as a function of chemical stress...
  18. pmc Random mutagenesis identifies novel genes involved in the secretion of antimicrobial, cell wall-lytic enzymes by Lactococcus lactis
    Yu Pei Tan
    Infectious Diseases Program, Cells and Tissue Domain, Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Queensland, Australia
    Appl Environ Microbiol 74:7490-6. 2008
    ....
  19. ncbi request reprint Control of dimethylsulfoxide reductase expression in Rhodobacter capsulatus: the role of carbon metabolites and the response regulators DorR and RegA
    Ulrike Kappler
    Department of Microbiology and Parasitology, School of Molecular and Microbial Sciences, The University of Queensland, Brisbane, QLD 4072, Australia
    Microbiology 148:605-14. 2002
    ..These data suggest that R. capsulatus contains a regulator of DMSO respiration that is distinct from DorR and RegA, is activated in the presence of pyruvate, and acts as a negative regulator of DMSO reductase expression...
  20. ncbi request reprint The multicopper oxidase of Pseudomonas aeruginosa is a ferroxidase with a central role in iron acquisition
    Wilhelmina M Huston
    Centre for Metals in Biology and Department of Microbiology and Parasitology, School of Molecular and Microbial Sciences, The University of Queensland, St Lucia, Australia
    Mol Microbiol 45:1741-50. 2002
    ..In view of the widespread distribution of the mco gene in bacteria, it is suggested that an iron acquisition mechanism involving multicopper oxidases may be an important and hitherto unrecognized feature of bacterial pathogenicity...