Genomes and Genes
Wilhelmina M Huston
Affiliation: Queensland University of Technology
- The IL-6 response to Chlamydia from primary reproductive epithelial cells is highly variable and may be involved in differential susceptibility to the immunopathological consequences of chlamydial infectionKelly Cunningham
Institute of Health and Biomedical Innovation, Queensland University of Technology, Q Block, 60 Musk Ave, Kelvin Grove, QLD 4059, Australia
BMC Immunol 14:50. 2013..This study aimed to investigate the role of primary human cellular responses to chlamydial stress response proteases and chlamydial infection to further identify the immune processes involved in serious disease sequelae...
- HtrA, RseP, and Tsp proteins do not elicit a pathology-related serum IgG response during sexually transmitted infection with Chlamydia trachomatisWilhelmina M Huston
Institute of Health and Biomedical Innovation and School of Life Sciences, Queensland University of Technology, Kelvin Grove, Qld 4053, Australia
J Reprod Immunol 85:168-71. 2010..Serum IgG(1-4) antibody subclasses reactive with these antigens was not significantly different between cohorts, although different responses to each antigen were detected...
- Unique residues involved in activation of the multitasking protease/chaperone HtrA from Chlamydia trachomatisWilhelmina M Huston
Institute of Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove, Queensland, Australia
PLoS ONE 6:e24547. 2011..Therefore, we have revealed novel residues involved in the activation of CtHtrA which are likely to have important in vivo implications for outer membrane protein assembly...
- Vaccination to protect against infection of the female reproductive tractWilhelmina M Huston
Institute of Health and Biomedical Innovation, 60 Musk Avenue, Queensland University of Technology, Kelvin Grove, Queensland, Australia
Expert Rev Clin Immunol 8:81-94. 2012..Despite these difficulties, there are numerous ongoing efforts to develop effective vaccines against these infectious agents and it is likely that this important human health field will see further major developments in the next 5 years...
- Bacterial proteases from the intracellular vacuole niche; protease conservation and adaptation for pathogenic advantageWilhelmina M Huston
Institute of Health and Biomedical Innovation and School of Life Sciences, Queensland University of Technology, Brisbane, QLD, Australia
FEMS Immunol Med Microbiol 59:1-10. 2010..This review summarizes the recent findings of novel protease functions from the intracellular human pathogenic bacteria that reside exclusively in vacuoles...
- Chlamydia trachomatis responds to heat shock, penicillin induced persistence, and IFN-gamma persistence by altering levels of the extracytoplasmic stress response protease HtrAWilhelmina M Huston
Institute of Health and Biomedical Innovation, 60 Musk Ave, Queensland University of Technology, Kelvin Grove, QLD, 4059, Australia
BMC Microbiol 8:190. 2008..Recombinant purified C. trachomatis HtrA has been previously shown to have both activities. This investigation examined the physiological role of Chlamydia trachomatis HtrA...
- Apoptosis is induced in Chlamydia trachomatis-infected HEp-2 cells by the addition of a combination innate immune activation compounds and the inhibitor wedelolactoneWilhelmina M Huston
Institute of Health and Biomedical Innovation and School of Life Sciences, Queensland University of Technology, Kelvin Grove, QLD, Australia
Am J Reprod Immunol 65:460-5. 2011..Innate immune activation of human cells, for some intracellular pathogens, is advantageous for vacuole morphology and pathogenic viability. It is unknown whether innate immune activation is advantageous to Chlamydia trachomatis viability...
- Expression and characterisation of a major c-type cytochrome encoded by gene kustc0563 from Kuenenia stuttgartiensis as a recombinant protein in Escherichia coliWilhelmina M Huston
Department of Biology Area 10, University of York, Heslington, York YO10 5YW, UK
Protein Expr Purif 51:28-33. 2007..The predicted protein sequence for the cytochrome suggests it to be a predominantly alpha-helical protein, and this is supported by circular dichroism...
- the active site residue V266 of Chlamydial HtrA is critical for substrate binding during both in vitro and in vivo conditionsSarina Gloeckl
Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, QLD, Australia
J Mol Microbiol Biotechnol 22:10-6. 2012..Notably, however, the interaction was significantly altered in 35 out of 69 clones when residue V266 was mutated, indicating that this residue has an important function during substrate binding...
- The temperature activated HtrA protease from pathogen Chlamydia trachomatis acts as both a chaperone and protease at 37 degrees CWilhelmina M Huston
Institute of Health and Biomedical Innovation and School of Life Sciences, Faculty of Science, Queensland University of Technology, Brisbane, Australia
FEBS Lett 581:3382-6. 2007..trachomatis HtrA functions as both a chaperone and protease at 37 degrees C. These properties are consistent with the developmental cycle of this obligate intracellular bacterium...
- Identification of a serine protease inhibitor which causes inclusion vacuole reduction and is lethal to Chlamydia trachomatisSarina Gloeckl
Institute of Health and Biomedical Innovation and School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, Kelvin Grove, QLD, 4059, Australia
Mol Microbiol 89:676-89. 2013....
- Characterization of in vitro Chlamydia muridarum persistence and utilization in an in vivo mouse model of Chlamydia vaccineAlison J Carey
Institute of Health and Biomedical Innovation, School of Biomedical Science, Faculty of Health, Queensland University of Technology, Brisbane, QLD, Australia
Am J Reprod Immunol 69:475-85. 2013..In vitro chlamydial persistence has been well characterized in various strains; however, there have been no reported studies in C. muridarum...
- Delivery of nitric oxide for analysis of the function of cytochrome c'Lindsay J Cole
Department of Biology, University of York, Heslington, York, United Kingdom
Methods Enzymol 436:21-33. 2008..We describe a number of techniques for steady-state UV/Vis spectrophotometry and pre-steady-state stopped-flow spectrophotometry analysis of cytochrome c'...
- The protease inhibitor JO146 demonstrates a critical role for CtHtrA for Chlamydia trachomatis reversion from penicillin persistenceVanissa A Ong
School of Biomedical Sciences, Institute of Health and Biomedical Innovation, Queensland University of Technology Brisbane, QLD, Australia
Front Cell Infect Microbiol 3:100. 2013..Thus, CtHtrA is an essential protease with both replicative phase and stress condition functions for Chlamydia trachomatis. ..
- Chlamydial infection of immune cells: altered function and implications for diseaseKenneth W Beagley
Institute of Health and Biomedical Innovation, Queensland University of Technology, Australia
Crit Rev Immunol 29:275-305. 2009..In this paper, we review the evidence for the growth of Chlamydia in immune cells, particularly monocytes/macrophages and dendritic cells, and describe how infection may affect the function of these cells...
- Heterologous overexpression and purification of cytochrome c' from Rhodobacter capsulatus and a mutant (K42E) in the dimerization region. Mutation does not alter oligomerization but impacts the heme iron spin state and nitric oxide binding propertiesWilhelmina M Huston
Department of Biology Area 10, University of York, Heslington, York, YO10 5YW, United Kingdom
Biochemistry 45:4388-95. 2006....
- Cytochrome P450 isozyme protein verified in the skin of southern hemisphere humpback whales (Megaptera novaeangliae): implications for biochemical biomarker assessmentCourtney A Waugh
The University of Queensland, The National Research Centre for Environmental Toxicology, Brisbane, QLD 4108, Australia
Mar Pollut Bull 62:758-61. 2011..The detection of CYP1A1 in the integument of the humpback whale affords the opportunity for further quantitative non-destructive investigations of enzyme activity as a function of chemical stress...
- Random mutagenesis identifies novel genes involved in the secretion of antimicrobial, cell wall-lytic enzymes by Lactococcus lactisYu Pei Tan
Infectious Diseases Program, Cells and Tissue Domain, Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Queensland, Australia
Appl Environ Microbiol 74:7490-6. 2008....
- Control of dimethylsulfoxide reductase expression in Rhodobacter capsulatus: the role of carbon metabolites and the response regulators DorR and RegAUlrike Kappler
Department of Microbiology and Parasitology, School of Molecular and Microbial Sciences, The University of Queensland, Brisbane, QLD 4072, Australia
Microbiology 148:605-14. 2002..These data suggest that R. capsulatus contains a regulator of DMSO respiration that is distinct from DorR and RegA, is activated in the presence of pyruvate, and acts as a negative regulator of DMSO reductase expression...
- The multicopper oxidase of Pseudomonas aeruginosa is a ferroxidase with a central role in iron acquisitionWilhelmina M Huston
Centre for Metals in Biology and Department of Microbiology and Parasitology, School of Molecular and Microbial Sciences, The University of Queensland, St Lucia, Australia
Mol Microbiol 45:1741-50. 2002..In view of the widespread distribution of the mco gene in bacteria, it is suggested that an iron acquisition mechanism involving multicopper oxidases may be an important and hitherto unrecognized feature of bacterial pathogenicity...