R J Whittington

Summary

Affiliation: New South Wales
Country: Australia

Publications

  1. ncbi request reprint Typing of IS 1311 polymorphisms confirms that bison (Bison bison) with paratuberculosis in Montana are infected with a strain of Mycobacterium avium subsp. paratuberculosis distinct from that occurring in cattle and other domesticated livestock
    R J Whittington
    NSW Agriculture, School of Veterinary Medicine, Elizabeth Macarthur Agricultural Institute, University of Pennsylvania, 382 West Street Road, PMB 8 Camden, New South Wales 2570, Australia
    Mol Cell Probes 15:139-45. 2001
  2. pmc Evaluation of modified BACTEC 12B radiometric medium and solid media for culture of Mycobacterium avium subsp. paratuberculosis from sheep
    R J Whittington
    NSW Agriculture, Elizabeth Macarthur Agricultural Institute, Camden, New South Wales 2570, Australia
    J Clin Microbiol 37:1077-83. 1999
  3. ncbi request reprint Further observations on the epidemiology and spread of epizootic haematopoietic necrosis virus (EHNV) in farmed rainbow trout Oncorhynchus mykiss in southeastern Australia and a recommended sampling strategy for surveillance
    R J Whittington
    NSW Agriculture, Elizabeth Macarthur Agricultural Institute, Australia
    Dis Aquat Organ 35:125-30. 1999
  4. pmc Use of pooled fecal culture for sensitive and economic detection of mycobacterium avium subsp. paratuberculosis infection in flocks of sheep
    R J Whittington
    NSW Agriculture, Elizabeth Macarthur Agricultural Institute, Camden, New South Wales 2570, Australia
    J Clin Microbiol 38:2550-6. 2000
  5. pmc Molecular epidemiology of Mycobacterium avium subsp. paratuberculosis: IS900 restriction fragment length polymorphism and IS1311 polymorphism analyses of isolates from animals and a human in Australia
    R J Whittington
    NSW Agriculture, Elizabeth Macarthur Agricultural Institute, Camden, New South Wales 2570, Australia
    J Clin Microbiol 38:3240-8. 2000
  6. ncbi request reprint Recovery of Mycobacterium avium subsp. paratuberculosis from nematode larvae cultured from the faeces of sheep with Johne's disease
    R J Whittington
    NSW Agriculture, Elizabeth Macarthur Agricultural Institute, PMB 8, NSW 2570, Camden, Australia
    Vet Microbiol 81:273-9. 2001
  7. ncbi request reprint Molecular epidemiological confirmation and circumstances of occurrence of sheep (S) strains of Mycobacterium avium subsp. paratuberculosis in cases of paratuberculosis in cattle in Australia and sheep and cattle in Iceland
    R J Whittington
    NSW Agriculture, Elizabeth Macarthur Agricultural Institute, NSW 2570, Camden, Australia
    Vet Microbiol 79:311-22. 2001
  8. ncbi request reprint Isolation of Mycobacterium avium subsp paratuberculosis from environmental samples collected from farms before and after destocking sheep with paratuberculosis
    R J Whittington
    NSW Agriculture, Elizabeth Macarthur Agricultural Institute, PMB 8, Camden, New South Wales 2570
    Aust Vet J 81:559-63. 2003
  9. ncbi request reprint Field evaluation of tracer sheep for the detection of early natural infection with Mycobacterium avium subsp paratuberculosis
    L A Reddacliff
    NSW Agriculture, EMAI, PMB 8 Camden, New South Wales
    Aust Vet J 82:426-33. 2004
  10. ncbi request reprint A preliminary study of possible genetic influences on the susceptibility of sheep to Johne's disease
    L A Reddacliff
    New South Wales Department of Primary Industries, PMB 8, Camden, New South Wales
    Aust Vet J 83:435-41. 2005

Collaborators

Detail Information

Publications43

  1. ncbi request reprint Typing of IS 1311 polymorphisms confirms that bison (Bison bison) with paratuberculosis in Montana are infected with a strain of Mycobacterium avium subsp. paratuberculosis distinct from that occurring in cattle and other domesticated livestock
    R J Whittington
    NSW Agriculture, School of Veterinary Medicine, Elizabeth Macarthur Agricultural Institute, University of Pennsylvania, 382 West Street Road, PMB 8 Camden, New South Wales 2570, Australia
    Mol Cell Probes 15:139-45. 2001
    ..The epidemiology of paratuberculosis in bison in Montana appears to be distinct from that in cattle and other farmed livestock investigated thus far...
  2. pmc Evaluation of modified BACTEC 12B radiometric medium and solid media for culture of Mycobacterium avium subsp. paratuberculosis from sheep
    R J Whittington
    NSW Agriculture, Elizabeth Macarthur Agricultural Institute, Camden, New South Wales 2570, Australia
    J Clin Microbiol 37:1077-83. 1999
    ..Both egg yolk and mycobactin J were essential additives for growth of ovine strains of M. avium subsp. paratuberculosis in both liquid and solid media...
  3. ncbi request reprint Further observations on the epidemiology and spread of epizootic haematopoietic necrosis virus (EHNV) in farmed rainbow trout Oncorhynchus mykiss in southeastern Australia and a recommended sampling strategy for surveillance
    R J Whittington
    NSW Agriculture, Elizabeth Macarthur Agricultural Institute, Australia
    Dis Aquat Organ 35:125-30. 1999
    ..Antigen-capture ELISA can be used as a cost effective screening test to detect EHNV on a farm provided that sampling rates conform with statistical principles...
  4. pmc Use of pooled fecal culture for sensitive and economic detection of mycobacterium avium subsp. paratuberculosis infection in flocks of sheep
    R J Whittington
    NSW Agriculture, Elizabeth Macarthur Agricultural Institute, Camden, New South Wales 2570, Australia
    J Clin Microbiol 38:2550-6. 2000
    ..It is recommended that pooled fecal culture replace serologic examination for detection of M. avium subsp. paratuberculosis infection at the flock level...
  5. pmc Molecular epidemiology of Mycobacterium avium subsp. paratuberculosis: IS900 restriction fragment length polymorphism and IS1311 polymorphism analyses of isolates from animals and a human in Australia
    R J Whittington
    NSW Agriculture, Elizabeth Macarthur Agricultural Institute, Camden, New South Wales 2570, Australia
    J Clin Microbiol 38:3240-8. 2000
    ..As these observations reflect past exposure of livestock to M. avium subsp. paratuberculosis, the monitoring of strains present in animals in Australia is continuing...
  6. ncbi request reprint Recovery of Mycobacterium avium subsp. paratuberculosis from nematode larvae cultured from the faeces of sheep with Johne's disease
    R J Whittington
    NSW Agriculture, Elizabeth Macarthur Agricultural Institute, PMB 8, NSW 2570, Camden, Australia
    Vet Microbiol 81:273-9. 2001
    ..Nematode parasites of sheep might be able to act as mechanical vectors for M. paratuberculosis as the organism associates with infective third stage larvae when these develop in the faeces of sheep with Johne's disease...
  7. ncbi request reprint Molecular epidemiological confirmation and circumstances of occurrence of sheep (S) strains of Mycobacterium avium subsp. paratuberculosis in cases of paratuberculosis in cattle in Australia and sheep and cattle in Iceland
    R J Whittington
    NSW Agriculture, Elizabeth Macarthur Agricultural Institute, NSW 2570, Camden, Australia
    Vet Microbiol 79:311-22. 2001
    ..In Iceland, different husbandry practices appear to have favoured transmission of S strains to cattle...
  8. ncbi request reprint Isolation of Mycobacterium avium subsp paratuberculosis from environmental samples collected from farms before and after destocking sheep with paratuberculosis
    R J Whittington
    NSW Agriculture, Elizabeth Macarthur Agricultural Institute, PMB 8, Camden, New South Wales 2570
    Aust Vet J 81:559-63. 2003
    ..A feasibility study and subsequent field survey...
  9. ncbi request reprint Field evaluation of tracer sheep for the detection of early natural infection with Mycobacterium avium subsp paratuberculosis
    L A Reddacliff
    NSW Agriculture, EMAI, PMB 8 Camden, New South Wales
    Aust Vet J 82:426-33. 2004
    ..To determine whether tracer sheep could be used to detect S strain Mycobacterium avium subsp paratuberculosis on pasture, and to provide further insight into the early stages of infection...
  10. ncbi request reprint A preliminary study of possible genetic influences on the susceptibility of sheep to Johne's disease
    L A Reddacliff
    New South Wales Department of Primary Industries, PMB 8, Camden, New South Wales
    Aust Vet J 83:435-41. 2005
    ..To investigate possible genetic influences on susceptibility or resistance of sheep to Johne's disease...
  11. ncbi request reprint Comparative sensitivity of various faecal culture methods and ELISA in dairy cattle herds with endemic Johne's disease
    G J Eamens
    NSW Agriculture, EMAI, PMB 8, NSW 2570, Camden, Australia
    Vet Microbiol 77:357-67. 2000
    ..paratuberculosis. In identifying shedder cattle, method 5 was the most sensitive, followed by methods 2, 4, 1, and 3 was the least sensitive. The number of BACTEC cultures confirmed by mycobactin dependence or PCR was similar...
  12. ncbi request reprint Pilus ELISA and an anamnestic test for the diagnosis of virulent ovine footrot and its application in a disease control program in Nepal
    O P Dhungyel
    Department of Veterinary Clinical Sciences, University of Sydney, NSW 2570, Camden, Australia
    Vet Microbiol 79:31-45. 2001
    ..The results supported clinical and bacteriological findings suggesting that virulent strains of D. nodosus have apparently been eliminated from these flocks in Nepal...
  13. doi request reprint Isolation of Mycobacterium avium subspecies paratuberculosis from muscle and peripheral lymph nodes using acid-pepsin digest prior to BACTEC culture
    L A Reddacliff
    New South Wales Department of Primary Industries, PMB 8, Camden NSW 2570, Australia
    Vet Microbiol 145:122-8. 2010
    ..a. paratuberculosis via meat may be small. Measures to prevent heavily infected animals, especially those with clinical signs, from entering the human food chain would further reduce this risk...
  14. ncbi request reprint Serogroup specific single and multiplex PCR with pre-enrichment culture and immuno-magnetic bead capture for identifying strains of D. nodosus in sheep with footrot prior to vaccination
    O P Dhungyel
    Faculty of Veterinary Science, University of Sydney, Camden, NSW 2570, Australia
    Mol Cell Probes 16:285-96. 2002
    ..Of these methods only PCR on mixed colonies from 4-day-old cultures on 4% hoof agar media yielded results of practical value...
  15. doi request reprint Cross species transmission of ovine Johne's disease from sheep to cattle: an estimate of prevalence in exposed susceptible cattle
    B J Moloney
    NSW Agriculture, Head Office, Locked Bag 21, Orange, NSW 2800, Australia
    Aust Vet J 86:117-23. 2008
    ..To determine the prevalence of infection of cattle with the sheep strain of Mycobacterium avium subsp paratuberculosis at least two years after exposure at < 6 months old...
  16. ncbi request reprint Progress towards understanding the spread, detection and control of Mycobacterium avium subsp paratuberculosis in animal populations
    R J Whittington
    New South Wales Agriculture, Elizabeth Macarthur Agricultural Institute, PMB 8 Camden, New South Wales 2570
    Aust Vet J 79:267-78. 2001
    ....
  17. ncbi request reprint Experimental infection of weaner sheep with S strain Mycobacterium avium subsp. paratuberculosis
    L A Reddacliff
    Faculty of Veterinary Science, University of Sydney, Private Bag 3, Camden, NSW 2570, Australia
    Vet Microbiol 96:247-58. 2003
    ..a. paratuberculosis, but low-level contamination may not be detected in such a system...
  18. ncbi request reprint The effect of decontamination protocols on the numbers of sheep strain Mycobacterium avium subsp. paratuberculosis isolated from tissues and faeces
    L A Reddacliff
    Elizabeth Macarthur Agricultural Institute, New South Wales Agriculture, PMB 8, Camden, NSW, Australia
    Vet Microbiol 95:271-82. 2003
    ..These studies may also facilitate future improvements to decontamination procedures...
  19. ncbi request reprint Surgical method for biopsy of terminal ileum and mesenteric lymph node of sheep for detection of Mycobacterium avium subsp paratuberculosis
    C S McConnel
    University of Sydney, Faculty of Veterinary Science, Camden, New South Wales 2570
    Aust Vet J 82:149-51. 2004
  20. doi request reprint Pilot trials in Australia on eradication of footrot by flock specific vaccination
    O P Dhungyel
    Faculty of Veterinary Science, The University of Sydney, Camden, NSW 2570, Australia
    Vet Microbiol 132:364-71. 2008
    ..This is the first study to report the successful use of specific vaccine for the intermediate form of footrot...
  21. ncbi request reprint Intrauterine and transmammary transmission of Mycobacterium avium subsp paratuberculosis in sheep
    C Lambeth
    Faculty of Veterinary Science, University of Sydney, Private Bag 3, Camden, New South Wales 2570
    Aust Vet J 82:504-8. 2004
    ..To investigate intrauterine infection of foetuses with Mycobacterium avium subsp paratuberculosis and the presence of infection in mammary secretions of sheep...
  22. ncbi request reprint Immunoperoxidase studies of cell mediated immune effector cell populations in early Mycobacterium avium subsp. paratuberculosis infection in sheep
    L A Reddacliff
    Faculty of Veterinary Science, University of Sydney, Private Bag 3, Camden, NSW 2570, Australia
    Vet Immunol Immunopathol 97:149-62. 2004
    ..These findings confirm and extend the peculiarities of the terminal ileal Peyer's patch in the young ruminant, with possible implications for the early establishment of M. a. paratuberculosis infection...
  23. ncbi request reprint Specificity of absorbed ELISA and agar gel immuno-diffusion tests for paratuberculosis in goats with observations about use of these tests in infected goats
    R J Whittington
    NSW Agriculture, Elizabeth Macarthur Agricultural Institute, Camden, New South Wales 2570
    Aust Vet J 81:71-5. 2003
    ..To determine the specificity of serological tests that are currently used in veterinary diagnostic laboratories in Australia for detection of Mycobacterium avium subsp paratuberculosis infection in goats...
  24. ncbi request reprint Progress towards a rapid polymerase chain reaction diagnostic test for the identification of Mycobacterium avium subsp. paratuberculosis in faeces
    I B Marsh
    Elizabeth Macarthur Agricultural Institute, NSW Agriculture, Menangle, New South Wales, Australia
    Mol Cell Probes 15:105-18. 2001
    ..Direct-PCR from faeces can be used as a rapid means of screening pooled faecal samples for flock diagnosis of Johne's disease in sheep...
  25. ncbi request reprint Sensitivity and specificity of pooled faecal culture and serology as flock-screening tests for detection of ovine paratuberculosis in Australia
    E S G Sergeant
    NSW Agriculture, Locked Bag 21, 2800, NSW, Orange, Australia
    Prev Vet Med 52:199-211. 2002
    ..Pooled faecal culture is performing at close to this level--whereas the flock-sensitivity of serology appears to be lower than expected, particularly in lower prevalence flocks...
  26. ncbi request reprint Deletion of an mmpL gene and multiple associated genes from the genome of the S strain of Mycobacterium avium subsp. paratuberculosis identified by representational difference analysis and in silico analysis
    I B Marsh
    Faculty of Veterinary Science, University of Sydney, Private Bag 3, Camden, NSW 2570, Australia
    Mol Cell Probes 19:371-84. 2005
    ....
  27. ncbi request reprint Rapid differentiation of Australian, European and American ranaviruses based on variation in major capsid protein gene sequence
    I B Marsh
    NSW Agriculture, Elizabeth Macarthur Agricultural Institute, Camden, NSW, Australia
    Mol Cell Probes 16:137-51. 2002
    ....
  28. doi request reprint Longitudinal study of clinicopathological features of Johne's disease in sheep naturally exposed to Mycobacterium avium subspecies paratuberculosis
    M M Dennis
    University of Sydney, Camden, NSW 2570, Australia
    Vet Pathol 48:565-75. 2011
    ..Six of the 46 (8.7%) biopsy-culture-positive sheep later had negative cultures at necropsy, suggesting recovery from infection. Further study is needed to identify factors associated with clearance of infection or progression of disease...
  29. ncbi request reprint Serological responses against the pathogenic dimorphic fungus Mucor amphibiorum in populations of platypus (Ornithorhynchus anatinus) with and without ulcerative mycotic dermatitis
    R J Whittington
    Faculty of Veterinary Science, University of Sydney, Private Mail Bag 3, NSW 2570, Camden, Australia
    Vet Microbiol 87:59-71. 2002
    ..Further studies are warranted to determine factors that result in progression from infection to disease, the occurrence of the fungus in areas where disease has not been observed and the specificity of antigen used in ELISA...
  30. ncbi request reprint Detection of antibodies against iridoviruses in the serum of the amphibian Bufo marinus
    R J Whittington
    NSW Agriculture, Elizabeth Macarthur Agricultural Institute, Menangle, Australia
    J Virol Methods 68:105-8. 1997
    ..Reactivity of cane toad antibodies against BIV, an amphibian virus, was greater than that against similar concentrations of EHNV, a piscine virus...
  31. ncbi request reprint Culture of pooled tissues for the detection of Mycobacterium avium subsp paratuberculosis infection in individual sheep
    L A Reddacliff
    NSW Agriculture, EMAI, PMB 8 Camden, New South Wales, 2570
    Aust Vet J 81:766-7. 2003
  32. ncbi request reprint Diagnosis of footrot in goats: application of ELISA tests for response to antigens of Dichelobacter nodosus
    S C Ghimire
    Faculty of Veterinary Science, University of Sydney, Camden, NSW 2570, Australia
    Vet Microbiol 87:237-51. 2002
    ..Therefore, they are only suitable for a herd diagnosis of footrot in goats and are dependent on the development of advanced under-running infections in a proportion of affected goats...
  33. doi request reprint Histopathological survey of lesions and infections affecting sick ornamental fish in pet shops in New South Wales, Australia
    S C Wickins
    Farm Animal and Veterinary Public Health, Faculty of Veterinary Science, University of Sydney, Camden, New South Wales 2570, Australia
    Dis Aquat Organ 94:143-52. 2011
    ..Those caring for sick ornamental fish should take appropriate steps to investigate infectious disease and should take precautions that prevent the spread of pathogens...
  34. ncbi request reprint Global trade in ornamental fish from an Australian perspective: the case for revised import risk analysis and management strategies
    R J Whittington
    Faculty of Veterinary Science, University of Sydney, PMB 3, Camden, NSW 2570, Australia
    Prev Vet Med 81:92-116. 2007
    ....
  35. ncbi request reprint Evaluation of an absorbed ELISA and an agar-gel immuno-diffusion test for ovine paratuberculosis in sheep in Australia
    E S G Sergeant
    AusVet Animal Health Services, 69 Turner Crescent, NSW 2800, Orange, Australia
    Prev Vet Med 61:235-48. 2003
    ....
  36. ncbi request reprint Evaluation of a Pourquier ELISA kit in relation to agar gel immunodiffusion (AGID) test for assessment of the humoral immune response in sheep and goats with and without Mycobacterium paratuberculosis infection
    S Gumber
    Faculty of Veterinary Science, The University of Sydney, Private Bag 3, Camden 2570, NSW, Australia
    Vet Microbiol 115:91-101. 2006
    ..0% were observed for positive and negative controls included on 193 plates over a 10-month period and there was a high level of intraassay repeatability with 12.0% of the duplicate samples having CV of >15.0%...
  37. ncbi request reprint Presence of Mycobacterium avium subspecies paratuberculosis in suspensions of ovine trichostrongylid larvae produced in faecal cultures artificially contaminated with the bacterium
    J B Lloyd
    Agricultural Institute, Camden NSW, Australia
    Vet Rec 148:261-3. 2001
    ..M. avium subspecies paratuberculosis was detected in the unirradiated larval suspensions and wash waters of all three nematode species, and in the irradiated H. contortus larval suspension...
  38. ncbi request reprint Comparison of BACTEC 460 and MGIT 960 systems for the culture of Mycobacterium avium subsp. paratuberculosis S strain and observations on the effect of inclusion of ampicillin in culture media to reduce contamination
    S Gumber
    The University of Sydney, Private Bag 3, Camden 2570, NSW, Australia
    Vet Microbiol 119:42-52. 2007
    ..The radiometric BACTEC culture system remains the best alternative for the culture of S strain and is recommended in circumstances where the genotype (s) of the strains present in a region/farm is either unknown or S strain...
  39. ncbi request reprint Molecular typing of Mycobacterium avium subspecies paratuberculosis strains from different hosts and regions
    I x Sevilla
    Instituto Vasco de Investigacion y Desarrollo Agrario NEIKER, Berreaga 1, 48160 Derio, Bizkaia, Spain
    Rev Sci Tech 24:1061-6. 2005
    ..These results show that there are genetic differences between Map isolates related to geographic and host factors that have a potential use in the epidemiological tracing of new paratuberculosis isolates...
  40. ncbi request reprint Longitudinal study of the spread of ovine Johne's disease in a sheep flock in southeastern New South Wales
    L Rast
    Gundagai Rural Lands Protection Board, PO Box 21, Gundagai New South Wales 2722
    Aust Vet J 83:227-32. 2005
    ..The aim of this study was to apply whole flock testing over time to determine the prevalence, distribution and spread of infection in a recently infected flock, with a view to planning intervention strategies for disease control...
  41. ncbi request reprint Genomic diversity in Mycobacterium avium: single nucleotide polymorphisms between the S and C strains of M. avium subsp. paratuberculosis and with M. a. avium
    I B Marsh
    Faculty of Veterinary Science, University of Sydney, Private Bag 3, Camden NSW 2570, Australia
    Mol Cell Probes 21:66-75. 2007
    ..An in silico comparison of these M. a. paratuberculosis sequences and the M. a. avium 104 genome revealed 86 SNPs, which corresponded well with similar studies of SNPs in the M. avium complex...
  42. ncbi request reprint Monte Carlo simulation of flock-level sensitivity of abattoir surveillance for ovine paratuberculosis
    K A Abbott
    Royal Veterinary College, Hawkshead Lane, North Mymms, AL9 7TA Herts, UK
    Prev Vet Med 61:309-32. 2003
    ..But-with relatively minor modifications of the protocol currently in use-it could become a key component of a surveillance programme which included additional testing strategies for small flocks...
  43. ncbi request reprint Evaluation of indigenous milk ELISA with m-culture and m-PCR for the diagnosis of bovine Johne's disease (BJD) in lactating Indian dairy cattle
    G Sharma
    Central Institute for Research on Goats, Makhdoom, 281 122 Farah, Mathura District, UP, India
    Res Vet Sci 84:30-7. 2008
    ..Map DNA isolated from milk samples of dairy cattle were first time genotyped as Map, 'Bison type' in India. High prevalence of Map in milk of dairy herds, posed major health hazard for calves and human beings...