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| R J WhittingtonSummaryAffiliation: New South Wales Country: Australia Publications
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Publications
Typing of IS 1311 polymorphisms confirms that bison (Bison bison) with paratuberculosis in Montana are infected with a strain of Mycobacterium avium subsp. paratuberculosis distinct from that occurring in cattle and other domesticated livestockR J Whittington
NSW Agriculture, School of Veterinary Medicine, Elizabeth Macarthur Agricultural Institute, University of Pennsylvania, 382 West Street Road, PMB 8 Camden, New South Wales 2570, Australia
Mol Cell Probes 15:139-45. 2001..The epidemiology of paratuberculosis in bison in Montana appears to be distinct from that in cattle and other farmed livestock investigated thus far...
Evaluation of modified BACTEC 12B radiometric medium and solid media for culture of Mycobacterium avium subsp. paratuberculosis from sheepR J Whittington
NSW Agriculture, Elizabeth Macarthur Agricultural Institute, Camden, New South Wales 2570, Australia
J Clin Microbiol 37:1077-83. 1999..Both egg yolk and mycobactin J were essential additives for growth of ovine strains of M. avium subsp. paratuberculosis in both liquid and solid media...- Further observations on the epidemiology and spread of epizootic haematopoietic necrosis virus (EHNV) in farmed rainbow trout Oncorhynchus mykiss in southeastern Australia and a recommended sampling strategy for surveillanceR J Whittington
NSW Agriculture, Elizabeth Macarthur Agricultural Institute, Australia
Dis Aquat Organ 35:125-30. 1999..Antigen-capture ELISA can be used as a cost effective screening test to detect EHNV on a farm provided that sampling rates conform with statistical principles... - Use of pooled fecal culture for sensitive and economic detection of mycobacterium avium subsp. paratuberculosis infection in flocks of sheepR J Whittington
NSW Agriculture, Elizabeth Macarthur Agricultural Institute, Camden, New South Wales 2570, Australia
J Clin Microbiol 38:2550-6. 2000..It is recommended that pooled fecal culture replace serologic examination for detection of M. avium subsp. paratuberculosis infection at the flock level... - Molecular epidemiology of Mycobacterium avium subsp. paratuberculosis: IS900 restriction fragment length polymorphism and IS1311 polymorphism analyses of isolates from animals and a human in AustraliaR J Whittington
NSW Agriculture, Elizabeth Macarthur Agricultural Institute, Camden, New South Wales 2570, Australia
J Clin Microbiol 38:3240-8. 2000..As these observations reflect past exposure of livestock to M. avium subsp. paratuberculosis, the monitoring of strains present in animals in Australia is continuing... - Recovery of Mycobacterium avium subsp. paratuberculosis from nematode larvae cultured from the faeces of sheep with Johne's diseaseR J Whittington
NSW Agriculture, Elizabeth Macarthur Agricultural Institute, PMB 8, NSW 2570, Camden, Australia
Vet Microbiol 81:273-9. 2001..Nematode parasites of sheep might be able to act as mechanical vectors for M. paratuberculosis as the organism associates with infective third stage larvae when these develop in the faeces of sheep with Johne's disease... - Molecular epidemiological confirmation and circumstances of occurrence of sheep (S) strains of Mycobacterium avium subsp. paratuberculosis in cases of paratuberculosis in cattle in Australia and sheep and cattle in IcelandR J Whittington
NSW Agriculture, Elizabeth Macarthur Agricultural Institute, NSW 2570, Camden, Australia
Vet Microbiol 79:311-22. 2001..In Iceland, different husbandry practices appear to have favoured transmission of S strains to cattle... - Isolation of Mycobacterium avium subsp paratuberculosis from environmental samples collected from farms before and after destocking sheep with paratuberculosisR J Whittington
NSW Agriculture, Elizabeth Macarthur Agricultural Institute, PMB 8, Camden, New South Wales 2570
Aust Vet J 81:559-63. 2003..However, improved culture methods are needed for critical surveys and to study the movement and fate of the organism in the environment... - Field evaluation of tracer sheep for the detection of early natural infection with Mycobacterium avium subsp paratuberculosisL A Reddacliff
NSW Agriculture, EMAI, PMB 8 Camden, New South Wales
Aust Vet J 82:426-33. 2004.... - A preliminary study of possible genetic influences on the susceptibility of sheep to Johne's diseaseL A Reddacliff
New South Wales Department of Primary Industries, PMB 8, Camden, New South Wales
Aust Vet J 83:435-41. 2005..CONCLUSION: If the results of this preliminary study are confirmed in further work, then the use of rams with "resistant" genotypes may assist in the control of Johne's disease in infected flocks... - Comparative sensitivity of various faecal culture methods and ELISA in dairy cattle herds with endemic Johne's diseaseG J Eamens
NSW Agriculture, EMAI, PMB 8, NSW 2570, Camden, Australia
Vet Microbiol 77:357-67. 2000..paratuberculosis. In identifying shedder cattle, method 5 was the most sensitive, followed by methods 2, 4, 1, and 3 was the least sensitive. The number of BACTEC cultures confirmed by mycobactin dependence or PCR was similar... - Pilus ELISA and an anamnestic test for the diagnosis of virulent ovine footrot and its application in a disease control program in NepalO P Dhungyel
Department of Veterinary Clinical Sciences, University of Sydney, NSW 2570, Camden, Australia
Vet Microbiol 79:31-45. 2001..The results supported clinical and bacteriological findings suggesting that virulent strains of D. nodosus have apparently been eliminated from these flocks in Nepal... 
Isolation of Mycobacterium avium subspecies paratuberculosis from muscle and peripheral lymph nodes using acid-pepsin digest prior to BACTEC cultureL A Reddacliff
New South Wales Department of Primary Industries, PMB 8, Camden NSW 2570, Australia
Vet Microbiol 145:122-8. 2010..a. paratuberculosis via meat may be small. Measures to prevent heavily infected animals, especially those with clinical signs, from entering the human food chain would further reduce this risk...- Serogroup specific single and multiplex PCR with pre-enrichment culture and immuno-magnetic bead capture for identifying strains of D. nodosus in sheep with footrot prior to vaccinationO P Dhungyel
Faculty of Veterinary Science, University of Sydney, Camden, NSW 2570, Australia
Mol Cell Probes 16:285-96. 2002..Of these methods only PCR on mixed colonies from 4-day-old cultures on 4% hoof agar media yielded results of practical value... - Cross species transmission of ovine Johne's disease from sheep to cattle: an estimate of prevalence in exposed susceptible cattleB J Moloney
NSW Agriculture, Head Office, Locked Bag 21, Orange, NSW 2800, Australia
Aust Vet J 86:117-23. 2008..To determine the prevalence of infection of cattle with the sheep strain of Mycobacterium avium subsp paratuberculosis at least two years after exposure at < 6 months old... - Progress towards understanding the spread, detection and control of Mycobacterium avium subsp paratuberculosis in animal populationsR J Whittington
New South Wales Agriculture, Elizabeth Macarthur Agricultural Institute, PMB 8 Camden, New South Wales 2570
Aust Vet J 79:267-78. 2001.... - Experimental infection of weaner sheep with S strain Mycobacterium avium subsp. paratuberculosisL A Reddacliff
Faculty of Veterinary Science, University of Sydney, Private Bag 3, Camden, NSW 2570, Australia
Vet Microbiol 96:247-58. 2003..a. paratuberculosis, but low-level contamination may not be detected in such a system... - The effect of decontamination protocols on the numbers of sheep strain Mycobacterium avium subsp. paratuberculosis isolated from tissues and faecesL A Reddacliff
Elizabeth Macarthur Agricultural Institute, New South Wales Agriculture, PMB 8, Camden, NSW, Australia
Vet Microbiol 95:271-82. 2003..These studies may also facilitate future improvements to decontamination procedures... - Surgical method for biopsy of terminal ileum and mesenteric lymph node of sheep for detection of Mycobacterium avium subsp paratuberculosisC S McConnel
University of Sydney, Faculty of Veterinary Science, Camden, New South Wales 2570
Aust Vet J 82:149-51. 2004 - Pilot trials in Australia on eradication of footrot by flock specific vaccinationO P Dhungyel
Faculty of Veterinary Science, The University of Sydney, Camden, NSW 2570, Australia
Vet Microbiol 132:364-71. 2008..This is the first study to report the successful use of specific vaccine for the intermediate form of footrot... - Intrauterine and transmammary transmission of Mycobacterium avium subsp paratuberculosis in sheepC Lambeth
Faculty of Veterinary Science, University of Sydney, Private Bag 3, Camden, New South Wales 2570
Aust Vet J 82:504-8. 2004..Therefore these modes of transmission are unlikely to compromise existing control programs for ovine Johne's disease on most farms, especially if programs include the immediate culling of clinically affected sheep... - Immunoperoxidase studies of cell mediated immune effector cell populations in early Mycobacterium avium subsp. paratuberculosis infection in sheepL A Reddacliff
Faculty of Veterinary Science, University of Sydney, Private Bag 3, Camden, NSW 2570, Australia
Vet Immunol Immunopathol 97:149-62. 2004..These findings confirm and extend the peculiarities of the terminal ileal Peyer's patch in the young ruminant, with possible implications for the early establishment of M. a. paratuberculosis infection... - Specificity of absorbed ELISA and agar gel immuno-diffusion tests for paratuberculosis in goats with observations about use of these tests in infected goatsR J Whittington
NSW Agriculture, Elizabeth Macarthur Agricultural Institute, Camden, New South Wales 2570
Aust Vet J 81:71-5. 2003..The two formats of ELISA evaluated in this study have similar characteristics and could be used in paratuberculosis control programs for the goat industries, but further data on sensitivity would increase confidence in their application... - Progress towards a rapid polymerase chain reaction diagnostic test for the identification of Mycobacterium avium subsp. paratuberculosis in faecesI B Marsh
Elizabeth Macarthur Agricultural Institute, NSW Agriculture, Menangle, New South Wales, Australia
Mol Cell Probes 15:105-18. 2001..Direct-PCR from faeces can be used as a rapid means of screening pooled faecal samples for flock diagnosis of Johne's disease in sheep... - Sensitivity and specificity of pooled faecal culture and serology as flock-screening tests for detection of ovine paratuberculosis in AustraliaE S G Sergeant
NSW Agriculture, Locked Bag 21, 2800, NSW, Orange, Australia
Prev Vet Med 52:199-211. 2002..Pooled faecal culture is performing at close to this level--whereas the flock-sensitivity of serology appears to be lower than expected, particularly in lower prevalence flocks... - Deletion of an mmpL gene and multiple associated genes from the genome of the S strain of Mycobacterium avium subsp. paratuberculosis identified by representational difference analysis and in silico analysisI B Marsh
Faculty of Veterinary Science, University of Sydney, Private Bag 3, Camden, NSW 2570, Australia
Mol Cell Probes 19:371-84. 2005.... - Rapid differentiation of Australian, European and American ranaviruses based on variation in major capsid protein gene sequenceI B Marsh
NSW Agriculture, Elizabeth Macarthur Agricultural Institute, Camden, NSW, Australia
Mol Cell Probes 16:137-51. 2002.... - Longitudinal study of clinicopathological features of Johne's disease in sheep naturally exposed to Mycobacterium avium subspecies paratuberculosisM M Dennis
University of Sydney, Camden, NSW 2570, Australia
Vet Pathol 48:565-75. 2011..Six of the 46 (8.7%) biopsy-culture-positive sheep later had negative cultures at necropsy, suggesting recovery from infection. Further study is needed to identify factors associated with clearance of infection or progression of disease... - Serological responses against the pathogenic dimorphic fungus Mucor amphibiorum in populations of platypus (Ornithorhynchus anatinus) with and without ulcerative mycotic dermatitisR J Whittington
Faculty of Veterinary Science, University of Sydney, Private Mail Bag 3, NSW 2570, Camden, Australia
Vet Microbiol 87:59-71. 2002..Further studies are warranted to determine factors that result in progression from infection to disease, the occurrence of the fungus in areas where disease has not been observed and the specificity of antigen used in ELISA... - Detection of antibodies against iridoviruses in the serum of the amphibian Bufo marinusR J Whittington
NSW Agriculture, Elizabeth Macarthur Agricultural Institute, Menangle, Australia
J Virol Methods 68:105-8. 1997..Reactivity of cane toad antibodies against BIV, an amphibian virus, was greater than that against similar concentrations of EHNV, a piscine virus... - Culture of pooled tissues for the detection of Mycobacterium avium subsp paratuberculosis infection in individual sheepL A Reddacliff
NSW Agriculture, EMAI, PMB 8 Camden, New South Wales, 2570
Aust Vet J 81:766-7. 2003 - Diagnosis of footrot in goats: application of ELISA tests for response to antigens of Dichelobacter nodosusS C Ghimire
Faculty of Veterinary Science, University of Sydney, Camden, NSW 2570, Australia
Vet Microbiol 87:237-51. 2002..Therefore, they are only suitable for a herd diagnosis of footrot in goats and are dependent on the development of advanced under-running infections in a proportion of affected goats... - Histopathological survey of lesions and infections affecting sick ornamental fish in pet shops in New South Wales, AustraliaS C Wickins
Farm Animal and Veterinary Public Health, Faculty of Veterinary Science, University of Sydney, Camden, New South Wales 2570, Australia
Dis Aquat Organ 94:143-52. 2011..Those caring for sick ornamental fish should take appropriate steps to investigate infectious disease and should take precautions that prevent the spread of pathogens... - Global trade in ornamental fish from an Australian perspective: the case for revised import risk analysis and management strategiesR J Whittington
Faculty of Veterinary Science, University of Sydney, PMB 3, Camden, NSW 2570, Australia
Prev Vet Med 81:92-116. 2007.... - Evaluation of an absorbed ELISA and an agar-gel immuno-diffusion test for ovine paratuberculosis in sheep in AustraliaE S G Sergeant
AusVet Animal Health Services, 69 Turner Crescent, NSW 2800, Orange, Australia
Prev Vet Med 61:235-48. 2003.... - Evaluation of a Pourquier ELISA kit in relation to agar gel immunodiffusion (AGID) test for assessment of the humoral immune response in sheep and goats with and without Mycobacterium paratuberculosis infectionS Gumber
Faculty of Veterinary Science, The University of Sydney, Private Bag 3, Camden 2570, NSW, Australia
Vet Microbiol 115:91-101. 2006..0% were observed for positive and negative controls included on 193 plates over a 10-month period and there was a high level of intraassay repeatability with 12.0% of the duplicate samples having CV of >15.0%... - Presence of Mycobacterium avium subspecies paratuberculosis in suspensions of ovine trichostrongylid larvae produced in faecal cultures artificially contaminated with the bacteriumJ B Lloyd
Agricultural Institute, Camden NSW, Australia
Vet Rec 148:261-3. 2001..M. avium subspecies paratuberculosis was detected in the unirradiated larval suspensions and wash waters of all three nematode species, and in the irradiated H. contortus larval suspension... - Comparison of BACTEC 460 and MGIT 960 systems for the culture of Mycobacterium avium subsp. paratuberculosis S strain and observations on the effect of inclusion of ampicillin in culture media to reduce contaminationS Gumber
The University of Sydney, Private Bag 3, Camden 2570, NSW, Australia
Vet Microbiol 119:42-52. 2007..The radiometric BACTEC culture system remains the best alternative for the culture of S strain and is recommended in circumstances where the genotype (s) of the strains present in a region/farm is either unknown or S strain... - Molecular typing of Mycobacterium avium subspecies paratuberculosis strains from different hosts and regionsI x Sevilla
, Berreaga 1, 48160 Derio, Bizkaia, Spain
Rev Sci Tech 24:1061-6. 2005..These results show that there are genetic differences between Map isolates related to geographic and host factors that have a potential use in the epidemiological tracing of new paratuberculosis isolates... - Longitudinal study of the spread of ovine Johne's disease in a sheep flock in southeastern New South WalesL Rast
Gundagai Rural Lands Protection Board, PO Box 21, Gundagai New South Wales 2722
Aust Vet J 83:227-32. 2005..The aim of this study was to apply whole flock testing over time to determine the prevalence, distribution and spread of infection in a recently infected flock, with a view to planning intervention strategies for disease control... - Genomic diversity in Mycobacterium avium: single nucleotide polymorphisms between the S and C strains of M. avium subsp. paratuberculosis and with M. a. aviumI B Marsh
Faculty of Veterinary Science, University of Sydney, Private Bag 3, Camden NSW 2570, Australia
Mol Cell Probes 21:66-75. 2007..An in silico comparison of these M. a. paratuberculosis sequences and the M. a. avium 104 genome revealed 86 SNPs, which corresponded well with similar studies of SNPs in the M. avium complex... - Monte Carlo simulation of flock-level sensitivity of abattoir surveillance for ovine paratuberculosisK A Abbott
Royal Veterinary College, Hawkshead Lane, North Mymms, AL9 7TA Herts, UK
Prev Vet Med 61:309-32. 2003..But-with relatively minor modifications of the protocol currently in use-it could become a key component of a surveillance programme which included additional testing strategies for small flocks... - Evaluation of indigenous milk ELISA with m-culture and m-PCR for the diagnosis of bovine Johne's disease (BJD) in lactating Indian dairy cattleG Sharma
Central Institute for Research on Goats, Makhdoom, 281 122 Farah, Mathura District, UP, India
Res Vet Sci 84:30-7. 2008..Map DNA isolated from milk samples of dairy cattle were first time genotyped as Map, 'Bison type' in India. High prevalence of Map in milk of dairy herds, posed major health hazard for calves and human beings...