Research Topics
Genomes and Genes | J C WhisstockSummaryAffiliation: Monash University Country: Australia Publications
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Detail Information
Publications
Hijacking of a substrate-binding protein scaffold for use in mycobacterial cell wall biosynthesisZara Marland
The Protein Crystallography Unit, Department of Biochemistry and Molecular Biology, School of Biomedical Sciences, Monash University, Clayton, VIC 3800, Australia
J Mol Biol 359:983-97. 2006....
An overview of the serpin superfamilyRuby H P Law
Department of Biochemistry and Molecular Biology, Monash University, Clayton Campus, Melbourne VIC 3800, Australia
Genome Biol 7:216. 2006..Serpins are conformationally labile and many of the disease-linked mutations of serpins result in misfolding or in pathogenic, inactive polymers...- The structure and function of catalytic domains within inositol polyphosphate 5-phosphatasesJ C Whisstock
Department of Biochemistry and Molecular Biology, Monash University, Victoria, Australia
IUBMB Life 53:15-23. 2002..The phosphoinositide polyphosphatase activity of the Sac1 domain, found in some inositol polyphosphate 5-phosphatases, is defined by a motif, CX5 R(T/S), also found in both protein and lipid phosphatases... - Serpins 2005 - fun between the beta-sheets. Meeting report based upon presentations made at the 4th International Symposium on Serpin Structure, Function and Biology (Cairns, Australia)James C Whisstock
The Department of Biochemistry and Molecular Biology, Monash University Clayton Campus, Melbourne, Victoria, Australia
FEBS J 272:4868-73. 2005..This review reports on recent major discoveries in the serpin field, based upon presentations made at the 4th International Symposium on Serpin Structure, Function and Biology (Cairns, Australia)... - Molecular gymnastics: serpin structure, folding and misfoldingJames C Whisstock
Protein Crystallography Unit, Department of Biochemistry and Molecular Biology, Clayton Campus, Melbourne 3800, Australia
Curr Opin Struct Biol 16:761-8. 2006..The cost of using metastability for function, however, is an increased propensity for misfolding... - The N terminus of the serpin, tengpin, functions to trap the metastable native stateQingwei Zhang
Department of Biochemistry and Molecular Biology, Monash University, Clayton, Melbourne, Victoria 3800, Australia
EMBO Rep 8:658-63. 2007..Therefore, the function of the N terminus in tengpin resembles protein cofactors that prevent mammalian serpins from spontaneously adopting the latent conformation... - Prediction of protein function from protein sequence and structureJames C Whisstock
Department of Biochemistry and Molecular Biology, Victorian Bioinformatics Consortium, Monash University, Clayton Campus, ARC Centre for Structural and Functional Microbial Genetics, Victoria, Australia
Q Rev Biophys 36:307-40. 2003..A fundamental problem is that function is in many cases an ill-defined concept. In this article we review the state of the art in function prediction and describe some of the underlying difficulties and successes... - The 1.6 A crystal structure of the catalytic domain of PlyB, a bacteriophage lysin active against Bacillus anthracisCorrine J Porter
Protein Crystallography Unit, Department of Biochemistry and Molecular Biology and The ARC Centre of Excellence for Structural and Functional Microbial Genomics, Monash University, Clayton, Melbourne, VIC 3800, Australia
J Mol Biol 366:540-50. 2007..PlyB therefore represents a new class of anthrax lysin and a new defensive tool in the armament against anthrax-mediated bioterrorism... - The murine orthologue of human antichymotrypsin: a structural paradigm for clade A3 serpinsAnita J Horvath
Australian Centre for Blood Diseases, Monash University, Commercial Road, 6th Floor, MacFarlane Burnet Building, Alfred Medical Research Precinct, Prahran, Victoria, 3181
J Biol Chem 280:43168-78. 2005.... - MUSTANG: a multiple structural alignment algorithmArun S Konagurthu
Department of Computer Science and Software Engineering, The University of Melbourne, Parkville, Melbourne, Victoria, 3010 Australia
Proteins 64:559-74. 2006.... - The high resolution crystal structure of a native thermostable serpin reveals the complex mechanism underpinning the stressed to relaxed transitionKate F Fulton
Protein Crystallography Unit, Monash Centre for Synchrotron Science, Department of Biochemistry and Molecular Biology, School of Biomedical Sciences, Faculty of Medicine, Victorian Bioinformatics Consortium, P O Box 53, Australia
J Biol Chem 280:8435-42. 2005..Here we have discussed the structural basis of how this serpin reconciles the thermodynamic instability necessary for function with the stability required to withstand elevated temperatures... - Characterization of an adapter subunit to a phosphatidylinositol (3)P 3-phosphatase: identification of a myotubularin-related protein lacking catalytic activityH H Nandurkar
Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria, Australia
Proc Natl Acad Sci U S A 98:9499-504. 2001..However, insect cell-expressed 3-PAP recombinant protein was catalytically inactive, confirming our prior prediction that this polypeptide represents an adapter subunit... - The X-ray crystal structure of Escherichia coli succinic semialdehyde dehydrogenase; structural insights into NADP+/enzyme interactionsChristopher G Langendorf
Department of Biochemistry and Molecular Biology, Monash University, Clayton Campus, Melbourne, Victoria, Australia
PLoS ONE 5:e9280. 2010..In Escherichia coli, two genetically distinct forms of SSADHs had been described that are essential for preventing accumulation of toxic levels of succinic semialdehyde (SSA) in cells... - Importance of the P4' residue in human granzyme B inhibitors and substrates revealed by scanning mutagenesis of the proteinase inhibitor 9 reactive center loopJ Sun
Department of Biochemistry and Molecular Biology, Monash University, 3800, Melbourne, Victoria, Australia
J Biol Chem 276:15177-84. 2001.... - The high resolution crystal structure of the human tumor suppressor maspin reveals a novel conformational switch in the G-helixRuby H P Law
The Protein Crystallography Unit, Monash Centre for Synchrotron Science and The Department of Biochemistry and Molecular Biology, School of Biomedical Sciences, Faculty of Medicine, Monash University, Clayton, Victoria 3800, Australia
J Biol Chem 280:22356-64. 2005..The high resolution crystal structure of maspin provides a detailed molecular framework to elucidate the mechanism of function of this important tumor suppressor... - A common fold mediates vertebrate defense and bacterial attackCarlos J Rosado
Department of Biochemistry and Molecular Biology, Monash University, Clayton, VIC 3800, Australia
Science 317:1548-51. 2007..Sequence similarity between bacterial and vertebrate MACPF domains suggests that the fold of the CDCs, a family of proteins important for bacterial pathogenesis, is probably used by vertebrates for defense against infection... - The 1.5 A crystal structure of a prokaryote serpin: controlling conformational change in a heated environmentJames A Irving
The Protein Crystallography Unit, Department of Biochemistry and Molecular Biology, School of Biomedical Sciences, Monash University, 3800, Clayton, Australia
Structure 11:387-97. 2003..These data provide evidence as to how this unusual serpin has adapted to fold and function in a heated environment... - Serpins in prokaryotesJames A Irving
Department of Biochemistry and Molecular Biology, Monash University, Melbourne, Victoria, Australia
Mol Biol Evol 19:1881-90. 2002..Using molecular modeling, we predict the means by which heat stability in the latter protein may be achieved without compromising inhibitory activity... - Alpha 1-antitrypsin polymerisation can occur by both loop A and C sheet mechanismsS P Bottomley
Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria, 3168, Australia
Biochem Biophys Res Commun 251:1-5. 1998..These results in conjunction with detailed molecular modelling studies, show that alpha1-antitrypsin is capable of undergoing both loop A-sheet and loop C-sheet mechanisms of polymerisation, depending upon the in vitro buffer conditions... - Glycoprotein Ib-IX-VR K Andrews
Vascular Biology Laboratory, Department of Biochemistry and Molecular Biology, Monash University, Clayton, VIC 3168, Australia
Int J Biochem Cell Biol 35:1170-4. 2003..Interactions involving GPIb-IX-V are therefore central to vascular processes of thrombosis and inflammation, and the receptor is under intense scrutiny as a potential therapeutic target... - IFI60/ISG60/IFIT4, a new member of the human IFI54/IFIT2 family of interferon-stimulated genesM J de Veer
Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria, 3168, Australia
Genomics 54:267-77. 1998..All four members of the human family are clustered together at chromosome 10q23.3. It is proposed that the four members of the IFI54 gene family evolved by a series of duplication events from a common gene of origin... 
Cascleave: towards more accurate prediction of caspase substrate cleavage sitesJiangning Song
Department of Biochemistry and Molecular Biology, Monash University, Melbourne, VIC 3800, Australia
Bioinformatics 26:752-60. 2010..Accordingly, systematic computational screening studies of caspase substrate cleavage sites may provide insight into the substrate specificity of caspases and further facilitating the discovery of putative novel substrates...- The human serpin proteinase inhibitor-9 self-associates at physiological temperaturesLauren N Benning
Department of Biochemistry and Molecular Biology, Monash University, P.O. Box 13D, Clayton, Victoria 3800, Australia
Protein Sci 13:1859-64. 2004..Our data show that in vitro wild-type PI-9 forms aggregates at physiological temperatures. The biological implications of PI-9 aggregates at physiological temperatures are discussed... - Molecular modeling of the seven tandem leucine-rich repeats within the ligand-binding region of platelet glycoprotein Ib alphaJames C Whisstock
Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria, Australia
Thromb Haemost 87:329-33. 2002..This provides the basis for relating these genetic lesions to abnormal function of the receptor... - GABA production by glutamic acid decarboxylase is regulated by a dynamic catalytic loopGustavo Fenalti
Department of Biochemistry and Molecular Biology, Monash University, Clayton, Melbourne, VIC 3800, Australia
Nat Struct Mol Biol 14:280-6. 2007..Kinetic studies suggest that mobility in the catalytic loop promotes a side reaction that results in cofactor release and GAD65 autoinactivation. These data reveal the molecular basis for regulation of GABA homeostasis... - Structure of the Plasmodium falciparum M17 aminopeptidase and significance for the design of drugs targeting the neutral exopeptidasesSheena McGowan
Department of Biochemistry and Molecular Biology, Monash University, Clayton Campus, Melbourne, Victoria 3800, Australia
Proc Natl Acad Sci U S A 107:2449-54. 2010..Our detailed understanding of the PfA-M1 and PfA-M17 active sites now permits a rational approach in the development of a unique class of two-target and/or combination antimalarial therapy... - Serpins: finely balanced conformational trapsRobert N Pike
Department of Biochemistry and Molecular Biology, Monash University, Melbourne, Victoria, Australia
IUBMB Life 54:1-7. 2002.... - Mechanisms of serpin dysfunction in diseaseDion Kaiserman
Department of Biochemistry and Molecular Biology, Monash University, Clayton, VIC 3800, Australia
Expert Rev Mol Med 8:1-19. 2006.... - PoPS: a computational tool for modeling and predicting protease specificitySarah E Boyd
School of Computer Science and Software Engineering and The Victorian Bioinformatics, Consortium, Monash University, Melbourne, Victoria 3800, Australia
J Bioinform Comput Biol 3:551-85. 2005..Furthermore, the tool also provides facilities to infer, compare and test models, and to store them in a publicly accessible database... - Serpins flex their muscle: II. Structural insights into target peptidase recognition, polymerization, and transport functionsJames C Whisstock
From the Department of Biochemistry and Molecular Biology and ARC Centre of Excellence in Structural and Functional Microbial Genomics, Monash University, Clayton, Victoria 3800, Australia
J Biol Chem 285:24307-12. 2010..Finally, recent structural studies reveal how the serpin fold has been adapted for non-inhibitory functions such as hormone binding... - The crystal structure of the transthyretin-like protein from Salmonella dublin, a prokaryote 5-hydroxyisourate hydrolaseSarah C Hennebry
The Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, Vic, Australia
J Mol Biol 359:1389-99. 2006..Together our data reveal that the active site of Sdu_TLP corresponds to the thyroxine binding site in TTRs... - Computational analysis of evolution and conservation in a protein superfamilyJames A Irving
Victorian Bioinformatics Consortium, and Department of Biochemistry and Molecular Biology, School of Biomedical Sciences, Monash University, Clayton, Vic, Australia
Methods 32:73-92. 2004..Reference is made to the serpin superfamily. The 'partition cluster' method, a relatively rapid technique for extracting underlying associations from phylogenetic bootstrap trees, is also presented... - The major human and mouse granzymes are structurally and functionally divergentDion Kaiserman
Department of Biochemistry and Molecular Biology and 2Victorian Bioinformatics Consortium, Monash University, Victoria 3800, Australia
J Cell Biol 175:619-30. 2006..These results demonstrate that even "orthologous" granzymes have species-specific functions, having evolved in distinct environments that pose different challenges... - Prodepth: predict residue depth by support vector regression approach from protein sequences onlyJiangning Song
Department of Biochemistry and Molecular Biology, Monash University, Clayton, Melbourne, Victoria, Australia
PLoS ONE 4:e7072. 2009..We also discuss the potential implications of this new structural parameter in the field of protein structure prediction and homology modeling. This method might prove to be a powerful tool for sequence analysis... - Introduction of a mutation in the shutter region of antithrombin (Phe77 --> Leu) increases affinity for heparin and decreases thermal stabilityNoelene S Quinsey
Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria 3800, Australia
Biochemistry 42:10169-73. 2003..This paper also demonstrates that there are major differences between the shutter regions of antithrombin and alpha(1)-antitrypsin since a stabilizing mutation in antitrypsin has the converse effect in antithrombin... - Structural basis for the inhibition of the essential Plasmodium falciparum M1 neutral aminopeptidaseSheena McGowan
Department of Biochemistry and Molecular Biology and Australian Research Council Centre of Excellence in Structural and Functional Microbial Genomics, Monash University, Clayton, Victoria, 3800, Australia
Proc Natl Acad Sci U S A 106:2537-42. 2009..These results set the groundwork for the development of antimalarial therapeutics that target the neutral aminopeptidases of the parasite... - The role of strand 1 of the C beta-sheet in the structure and function of alpha(1)-antitrypsinS P Bottomley
Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria 3800, Australia
Protein Sci 10:2518-24. 2001..The results of this study allow more informed analysis of the effects of mutations found at these positions in disease-associated serpin variants... - A structural basis for loop C-sheet polymerization in serpinsQingwei Zhang
Department of Biochemistry and Molecular Biology, Monash University, Clayton, Melbourne, VIC 3800, Australia
J Mol Biol 376:1348-59. 2008..Polymerization of latent neuroserpin may be important for the development of familial encephalopathy with neuroserpin inclusion bodies. Our structural data provide a possible mechanism for polymerization by latent serpins... - Structure of granzyme C reveals an unusual mechanism of protease autoinhibitionDion Kaiserman
Department of Biochemistry and Molecular Biology, Monash University, Clayton 3800, Australia
Proc Natl Acad Sci U S A 106:5587-92. 2009..Overall, these observations describe a broadly applicable mechanism of protease regulation that cannot be predicted by template-based modeling or bioinformatic approaches alone... - Cationic sites on granzyme B contribute to cytotoxicity by promoting its uptake into target cellsCatherina H Bird
Department of Biochemistry and Molecular Biology, Monash University, Melbourne, Australia
Mol Cell Biol 25:7854-67. 2005..In this electrostatic "exchange-adsorption" model, cs1 and cs2 participate in binding of GrB to the cell surface, thereby promoting its uptake and eventual release into the cytoplasm... - Structural determinants of GAD antigenicityYasir Arafat
The Department of Biochemistry and Molecular Biology, Monash University, Clayton, Melbourne, VIC 3800, Australia
Mol Immunol 47:493-505. 2009..This could well apply to the various other enzyme autoantigens and, if so, these features could be used as the basis of future predictive strategies... - Evidence that serpin architecture intrinsically supports papain-like cysteine protease inhibition: engineering alpha(1)-antitrypsin to inhibit cathepsin proteasesJames A Irving
Department of Biochemistry and Molecular Biology, Monash University Clayton Campus, P.O. Box 13D, Victoria 3800, Australia
Biochemistry 41:4998-5004. 2002.... - Probing the role of the F-helix in serpin stability through a single tryptophan substitutionLisa D Cabrita
Department of Biochemistry and Molecular Biology, Monash University, P.O. Box 13D, Victoria 3800, Australia
Biochemistry 41:4575-81. 2002..The implications of these data on both alpha1-antitrypsin function and misfolding are discussed... - X-ray crystal structure of MENT: evidence for functional loop-sheet polymers in chromatin condensationSheena McGowan
Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria, Australia
EMBO J 25:3144-55. 2006.... - Characterization of the specificity of arginine-specific gingipains from Porphyromonas gingivalis reveals active site differences between different forms of the enzymesNafisa Ally
Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria 3800, Australia
Biochemistry 42:11693-700. 2003.... - The yeast inositol polyphosphate 5-phosphatase Inp54p localizes to the endoplasmic reticulum via a C-terminal hydrophobic anchoring tail: regulation of secretion from the endoplasmic reticulumF Wiradjaja
Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria 3800, Australia
J Biol Chem 276:7643-53. 2001..We propose that Inp54p plays a role in regulating secretion, possibly by modulating the levels of phosphatidylinositol (4,5)-bisphosphate on the cytoplasmic surface of the endoplasmic reticulum membrane... - Cleaved antitrypsin polymers at atomic resolutionM A Dunstone
The Ian Potter Foundation Protein Crystallography Laboratory, St Vincent s Institute of Medical Research, Fitzroy, Victoria, Australia
Protein Sci 9:417-20. 2000..Here, we describe the first crystallographic evidence of a beta-strand linked polymer form of alpha1-antitrypsin: the crystal structure of a cleaved alpha1-antitrypsin polymer... - DNA accelerates the inhibition of human cathepsin V by serpinsPoh Chee Ong
Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria 3800, Australia
J Biol Chem 282:36980-6. 2007..This represents the first example of modulation of serpin inhibition of cysteine proteases by a co-factor and reveals a mechanism for differential regulation of cathepsin proteolytic activity in a DNA-rich environment... - Modulation of the proteolytic activity of the complement protease C1s by polyanions: implications for polyanion-mediated acceleration of interaction between C1s and SERPING1Thomas A Murray-Rust
Department of Biochemistry and Molecular Biology, Monash University, Wellington Road, Clayton, Victoria 3800, Australia
Biochem J 422:295-303. 2009..Thus, whereas polyanions are able to bind C1s and modulate its activity, polyanion interactions with SERPING1 must also play a vital role in the mechanism by which these cofactors accelerate the C1s-SERPING1 reaction... - EGM: encapsulated gene-by-gene matching to identify gene orthologs and homologous segments in genomesKhalid Mahmood
Department of Biochemistry and Molecular Biology, Monash University, Clayton, VIC 3800, Australia
Bioinformatics 26:2076-84. 2010..Such an insight is particularly useful, for example, in the transfer of experimental results between different experimental systems such as Drosophila and mammals... - COOH-terminal clustering of autoantibody and T-cell determinants on the structure of GAD65 provide insights into the molecular basis of autoreactivityGustavo Fenalti
Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria 3800, Australia
Diabetes 57:1293-301. 2008.... - Aeropin from the extremophile Pyrobaculum aerophilum bypasses the serpin misfolding trapLisa D Cabrita
Department of Biochemistry and Molecular Biology, Monash University, Victoria 3800, Australia
J Biol Chem 282:26802-9. 2007.... - Molecular determinants of the mechanism underlying acceleration of the interaction between antithrombin and factor Xa by heparin pentasaccharideNoelene S Quinsey
Department of Biochemistry and Molecular Biology, School of Biomedical Sciences, Monash University, Clayton, Victoria 3800, Australia
J Biol Chem 277:15971-8. 2002.... - The evolution of enzyme specificity in Fasciola sppJames A Irving
Department of Biochemistry and Molecular Biology, Monash University, Victoria 3800, Australia
J Mol Evol 57:1-15. 2003..A number of other positions subject to evolutionary pressure and potentially significant for enzyme function are also identified, including sites anticipated to diminish cystatin binding affinity... - Elucidation of the substrate specificity of the C1s protease of the classical complement pathwayFelicity K Kerr
Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria 3800, Australia
J Biol Chem 280:39510-4. 2005.... - Prediction of the immunodominant epitope of the pyruvate dehydrogenase complex E2 in primary biliary cirrhosis using phage displayM J Rowley
Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria, Australia
J Immunol 164:3413-9. 2000..The peptide sequences, along with the known NMR structure of the inner lipoyl domain of PDC-E2, allow the prediction of nonsequential residues 131HM132 and 178FEV180 that contribute to a conformational epitope... - Protein structural alignments and functional genomicsJ A Irving
Department of Biochemistry and Molecular Biology, Monash University, Clayton Campus, Melbourne, Victoria, Australia
Proteins 42:378-82. 2001..In the region of small common substructures, reduced aligned subsets define active sites and can be used to suggest the locations of active sites in homologous proteins... - The structure and function of mammalian membrane-attack complex/perforin-like proteinsS C Kondos
Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria, Australia
Tissue Antigens 76:341-51. 2010..A current challenge is thus to understand the role, pore forming or otherwise, of MACPF proteins in developmental biology. This review discusses structural and functional diversity of the mammalian MACPF proteins... - X-ray crystal structure of the fibrinolysis inhibitor alpha2-antiplasminRuby H P Law
Department of Biochemistry and Molecular Biology, Monash University, Clayton, Australia
Blood 111:2049-52. 2008..This would be anticipated to position the flexible plasmin-binding portion of the C-terminus in close proximity to the serpin Reactive Center Loop where it may act as a template to accelerate serpin/protease interactions... - The MACPF/CDC family of pore-forming toxinsCarlos J Rosado
Department of Biochemistry, Monash University, Clayton, Victoria 3800, Australia
Cell Microbiol 10:1765-74. 2008..Together with biochemical studies, these structural data suggest that lytic MACPF proteins use a CDC-like mechanism of membrane disruption, and will help understand the roles these proteins play in immunity and development... - Federated repositories of X-ray diffraction imagesSteve Androulakis
The Department of Biochemistry and Molecular Biology, School of Biomedical Sciences, Faculty of Medicine, Monash University, Clayton, Victoria 3800, Australia
Acta Crystallogr D Biol Crystallogr . 2008..Moreover, the availability of raw data is extremely useful for the development of improved methods of image analysis and data processing... - The REFOLD database: a tool for the optimization of protein expression and refoldingMichelle K M Chow
Department of Biochemistry and Molecular Biology, PO Box 13D, Monash University, Victoria 3800, Australia
Nucleic Acids Res 34:D207-12. 2006..We anticipate that REFOLD will continue to grow and eventually become a powerful tool for the optimization of protein renaturation. REFOLD is freely available at http://refold.med.monash.edu.au... - Determination of the P1', P2' and P3' subsite-specificity of factor XaJustin P Ludeman
Department of Biochemistry and Molecular Biology, School of Biomedical Sciences, Monash University, P.O. Box 13D, Clayton, Vic. 3800, Australia
Int J Biochem Cell Biol 35:221-5. 2003..It appears that while the prime side subsites of factor Xa impose some selectivity towards substrates, the influence of these sites on factor Xa cleavage specificity is relatively low in comparison to related enzymes such as thrombin... - Subunit gamma-green fluorescent protein fusions are functionally incorporated into mitochondrial F1F0-ATP synthase, arguing against a rigid cap structure at the top of F1Mark Prescott
Department of Biochemistry and Molecular Biology, P. O. Box 13D, Monash University, Clayton Campus, Victoria 3800, Australia
J Biol Chem 278:251-6. 2003..Our conclusion is that the putative cap structure cannot be an inflexible structure, but must be of a more flexible nature consistent with the accommodation of subunit gamma-GFP fusions within functional ATP synthase complexes... - Maspin (SERPINB5) is an obligate intracellular serpinSonia S Y Teoh
Department of Biochemistry and Molecular Biology, School of Biomedical Sciences, Monash University, Victoria 3800, Australia
J Biol Chem 285:10862-9. 2010..It is probably released only as a consequence of cell damage or necrosis... - Importance of the prime subsites of the C1s protease of the classical complement pathway for recognition of substratesGrace O'Brien
Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria 3800, Australia
Biochemistry 42:14939-45. 2003.... - Antithrombin: in control of coagulationNoelene S Quinsey
Department of Biochemistry and Molecular Biology, Monash University, Clayton, Vic. 3800, Australia
Int J Biochem Cell Biol 36:386-9. 2004..The clinical importance of antithrombin is manifested by its clear association with thrombosis when deficiency states occur... - Conformational change in the chromatin remodelling protein MENTPoh Chee Ong
Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria, Australia
PLoS ONE 4:e4727. 2009.... - T cell receptor recognition of a 'super-bulged' major histocompatibility complex class I-bound peptideFleur E Tynan
The Protein Crystallography Unit, Department of Biochemistry and Molecular Biology, School of Biomedical Sciences, Monash University, Clayton, Victoria 3800, Australia
Nat Immunol 6:1114-22. 2005..Thus our findings simultaneously demonstrate the considerable adaptability of the TCR and the 'shape' of MHC restriction... - REFOLD: an analytical database of protein refolding methodsMichelle K M Chow
Department of Biochemistry and Molecular Biology, P.O. Box 13D, Monash University, Vic. 3800, Australia
Protein Expr Purif 46:166-71. 2006.... - Structural and functional analysis of the Josephin domain of the polyglutamine protein ataxin-3Michelle K M Chow
Department of Biochemistry and Molecular Biology, P.O. Box 13D, Monash University, Vic. 3800, Australia
Biochem Biophys Res Commun 322:387-94. 2004..However, its presence destabilizes the Josephin domain. The implications of these data in the pathogenesis of polyglutamine repeat proteins are discussed... - Leucine-rich repeats 2-4 (Leu60-Glu128) of platelet glycoprotein Ibalpha regulate shear-dependent cell adhesion to von Willebrand factorYang Shen
Department of Immunology and the Department of Biochemistry and Molecular Biology, Monash University, Victoria, 3800, Australia
J Biol Chem 281:26419-23. 2006..Together, these data demonstrate that LRR2-4, encompassing a pronounced negative charge patch on human GPIbalpha, is essential for GPIbalpha.VWF-dependent adhesion as hydrodynamic shear increases... - Managing and mining protein crystallization dataAbdullah A Amin
Victorian Bioinformatics Consortium, Monash University, Clayton, Victoria, Australia
Proteins 62:4-7. 2006..The CLIMS2 executable and documentation is freely available at http://clims.med.monash.edu.au... - RCPdb: An evolutionary classification and codon usage database for repeat-containing proteinsNoel G Faux
Protein Crystallography Unit, Department of Biochemistry and Molecular Biology, Monash University, Melbourne, Victoria, Australia
Genome Res 17:1118-27. 2007..Third, homopeptides that are conserved across different species lie within regions that are under stronger purifying selection in contrast to nonconserved homopeptides... - Mutagenesis of the dengue virus type 2 NS3 proteinase and the production of growth-restricted virusA E Matusan
Department of Microbiology and Department of Biochemistry and Molecular Biology, Monash University, PO Box 53, Victoria 3800, Australia
J Gen Virol 82:1647-56. 2001..The study demonstrated that charged-to-alanine mutagenesis in the viral proteinase can be used to produce growth-restricted flaviviruses that may be useful in the production of attenuated vaccine strains... - The FxRxHrS motif: a conserved region essential for DNA binding of the VirR response regulator from Clostridium perfringensSheena McGowan
Bacterial Pathogenesis Research Group, Department of Microbiology, Monash University, 3800 Australia
J Mol Biol 322:997-1011. 2002..These studies have important implications for this new family of transcriptional factors since they imply that the conserved FxRxHrS motif may be involved in DNA binding in all of these proteins, irrespective of their biological role... - The serpin SQN-5 is a dual mechanistic-class inhibitor of serine and cysteine proteinasesMay Al-Khunaizi
Department of Pediatrics, The Harvard Medical School, Children s Hospital, 300 Longwood Avenue, Enders 970, Boston, Massachusetts 02115, USA
Biochemistry 41:3189-99. 2002..When the fact that mammals and birds diverged approximately 310 million years ago is considered, an ancestral SCCA/SQN-like serpin with dual inhibitory activity may be present in many mammalian genomes... - The matrix refoldedAshley M Buckle
Nat Methods 2:3. 2005 - The crystal structure of DehI reveals a new alpha-haloacid dehalogenase fold and active-site mechanismJason W Schmidberger
Department of Pharmacology, School of Medicine and Pharmacology, The University of Western Australia, Perth, Western Australia 6907, Australia
J Mol Biol 378:284-94. 2008..These details will assist with future bioengineering of dehalogenases... - A serpin in the cellulosome of the anaerobic fungus Piromyces sp. strain E2Peter J M Steenbakkers
Department of Microbiology, IWWR, Radboud University Nijmegen, Toernooiveld 1, NL 6525ED Nijmegen, The Netherlands
Mycol Res 112:999-1006. 2008..strain E2 is the first non-structural, non-hydrolytic fungal cellulosome component. Furthermore, the celpin protein of Piromyces sp. strain E2 is the first representative of a serine proteinase inhibitor of the fungal kingdom... - Role of the M-loop and reactive center loop domains in the folding and bridging of nucleosome arrays by MENTEvelyn M Springhetti
Department of Biochemistry and Molecular Biology, Milton S. Hershey Medical Center, Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033, USA
J Biol Chem 278:43384-93. 2003..Ordered binding of MENT to linker DNA via its unique M-loop domain promotes the folding of chromatin, whereas bridging of chromatin fibers is facilitated by MENT oligomerization mediated by the RCL... - Inhibitory activity of a heterochromatin-associated serpin (MENT) against papain-like cysteine proteinases affects chromatin structure and blocks cell proliferationJames A Irving
Department of Biochemistry and Molecular Biology, Clayton Campus, Monash University, P. O. Box 13D, Victoria 3800, Australia
J Biol Chem 277:13192-201. 2002..We conclude that the repressive effect of MENT on chromatin is mediated by its direct interaction with a nuclear protein that has a papain-like cysteine proteinase active site... - AB5 subtilase cytotoxin inactivates the endoplasmic reticulum chaperone BiPAdrienne W Paton
School of Molecular and Biomedical Science, University of Adelaide, South Australia 5005, Australia
Nature 443:548-52. 2006..BiP is a master regulator of endoplasmic reticulum function, and its cleavage by subtilase cytotoxin represents a previously unknown trigger for cell death... - A structural basis for the selection of dominant alphabeta T cell receptors in antiviral immunityLars Kjer-Nielsen
Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria 3010, Australia
Immunity 18:53-64. 2003..These findings indicate that TCR immunodominance is associated with structural properties conferring receptor specificity and suggest a novel structural link between TCR ligation and intracellular signaling... - Functional insights from the distribution and role of homopeptide repeat-containing proteinsNoel G Faux
Protein Crystallography Unit, Department of Biochemistry and Molecular Biology, School of Computer Science and Software Engineering, Monash University, Clayton Campus, Melbourne, VIC 3800, Australia
Genome Res 15:537-51. 2005..These data reveal that the majority of RCPs are involved in processes that require the assembly of large, multiprotein complexes, such as transcription and signaling... - Identification of a novel domain in two mammalian inositol-polyphosphate 5-phosphatases that mediates membrane ruffle localization. The inositol 5-phosphatase skip localizes to the endoplasmic reticulum and translocates to membrane ruffles following epideRajendra Gurung
Department of Biochemistry and Molecular Biology, Monash University, Victoria 3800, Australia
J Biol Chem 278:11376-85. 2003..Collectively, these studies demonstrate a novel membrane-targeting domain that serves to recruit SKIP and PIPP to membrane ruffles... - The type Ialpha inositol polyphosphate 4-phosphatase generates and terminates phosphoinositide 3-kinase signals on endosomes and the plasma membraneIvan Ivetac
Department of Biochemistry and Molecular Biology, Monash University, Clayton, Australia 3800
Mol Biol Cell 16:2218-33. 2005..The 4-phosphatase therefore both generates and terminates phosphoinositide 3-kinase signals at distinct subcellular locations... - Hurpin is a selective inhibitor of lysosomal cathepsin L and protects keratinocytes from ultraviolet-induced apoptosisThomas Welss
Department of Dermatology, Heinrich Heine University, D 40225 Dusseldorf, Germany
Biochemistry 42:7381-9. 2003..Given that lysosomal disruption, release of catL, and catL-mediated caspase activation are known to occur in response to cellular stress, we propose that a physiological role of hurpin is to protect epithelial cells from ectopic catL... - SERPINB11 is a new noninhibitory intracellular serpin. Common single nucleotide polymorphisms in the scaffold impair conformational changeDavid J Askew
University of Pittsburgh Medical Center Newborn Medicine Program, Children s Hospital of Pittsburgh and Magee Womens Research Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
J Biol Chem 282:24948-60. 2007..Like several other serpin superfamily members, SERPINB11 has lost inhibitory activity and may have evolved a noninhibitory function... - SerpinB6 is an inhibitor of kallikrein-8 in keratinocytesFiona L Scott
Department of Biochemistry and Molecular Biology, School of Biomedical Sciences, Monash University, Victoria 3800, Australia
J Biochem 142:435-42. 2007..Taken together, these results suggest that serpinB6 is a physiologically relevant inhibitor of hK8 in skin. We postulate that serpinB6 protects the intracellular compartment of keratinocytes from ectopic hK8... - Elucidation of the substrate specificity of the MASP-2 protease of the lectin complement pathway and identification of the enzyme as a major physiological target of the serpin, C1-inhibitorFelicity K Kerr
Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria 3800, Australia
Mol Immunol 45:670-7. 2008..C1-inhibitor inhibited MASP-2 50-fold faster than C1s and much faster than any other protease tested to date, implying that MASP-2 is a major physiological target of C1-inhibitor...