T Stinear

Summary

Affiliation: Monash University
Country: Australia

Publications

  1. pmc Different bacterial gene expression patterns and attenuated host immune responses are associated with the evolution of low-level vancomycin resistance during persistent methicillin-resistant Staphylococcus aureus bacteraemia
    Benjamin P Howden
    Australian Bacterial Pathogenesis Program, Department of Microbiology, Monash University, Clayton, Victoria, Australia
    BMC Microbiol 8:39. 2008
  2. pmc Deciphering the genetic basis for polyketide variation among mycobacteria producing mycolactones
    Sacha J Pidot
    Department of Microbiology, Monash University, Clayton, 3800, Australia
    BMC Genomics 9:462. 2008
  3. pmc Evolution of two distinct phylogenetic lineages of the emerging human pathogen Mycobacterium ulcerans
    Michael Kaser
    Swiss Tropical Institute, Socinstr, 57, 4002 Basel, Switzerland
    BMC Evol Biol 7:177. 2007
  4. ncbi request reprint Functional analysis and annotation of the virulence plasmid pMUM001 from Mycobacterium ulcerans
    Timothy P Stinear
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, 28 rue du Docteur Roux, 75725 Paris Cedex 15, France
    Microbiology 151:683-92. 2005
  5. pmc Common evolutionary origin for the unstable virulence plasmid pMUM found in geographically diverse strains of Mycobacterium ulcerans
    Timothy P Stinear
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, Paris, France
    J Bacteriol 187:1668-76. 2005
  6. pmc Comparative genetic analysis of Mycobacterium ulcerans and Mycobacterium marinum reveals evidence of recent divergence
    T P Stinear
    Bacterial Pathogenesis Research Group, Department of Microbiology, Monash University, Clayton, Victoria, Australia
    J Bacteriol 182:6322-30. 2000
  7. pmc A simple PCR method for rapid genotype analysis of Mycobacterium ulcerans
    T Stinear
    Department of Microbiology, Monash University, Victoria, Australia
    J Clin Microbiol 38:1482-7. 2000
  8. pmc Identification and characterization of IS2404 and IS2606: two distinct repeated sequences for detection of Mycobacterium ulcerans by PCR
    T Stinear
    Department of Microbiology, Monash University, Clayton, Victoria, Australia
    J Clin Microbiol 37:1018-23. 1999
  9. pmc Insights from the complete genome sequence of Mycobacterium marinum on the evolution of Mycobacterium tuberculosis
    Timothy P Stinear
    Department of Microbiology, Monash University, Clayton 3800, Australia
    Genome Res 18:729-41. 2008
  10. pmc Detection of Mycobacterium ulcerans in environmental samples during an outbreak of ulcerative disease
    B C Ross
    Department of Microbiology and Infectious Disease, Royal Children s Hospital, Melbourne, Victoria, Australia
    Appl Environ Microbiol 63:4135-8. 1997

Collaborators

Detail Information

Publications31

  1. pmc Different bacterial gene expression patterns and attenuated host immune responses are associated with the evolution of low-level vancomycin resistance during persistent methicillin-resistant Staphylococcus aureus bacteraemia
    Benjamin P Howden
    Australian Bacterial Pathogenesis Program, Department of Microbiology, Monash University, Clayton, Victoria, Australia
    BMC Microbiol 8:39. 2008
    ..Based on the transcriptome results multiple genes were sequenced and innate immune system stimulation was assessed in the VSSA and hVISA/VISA pairs...
  2. pmc Deciphering the genetic basis for polyketide variation among mycobacteria producing mycolactones
    Sacha J Pidot
    Department of Microbiology, Monash University, Clayton, 3800, Australia
    BMC Genomics 9:462. 2008
    ..In M. ulcerans strain Agy99, mycolactone A/B is produced by three highly homologous type I polyketide megasynthases (PKS), whose genes (mlsA1: 51 kb, mlsA2: 7.2 kb and mlsB: 42 kb) are found on a 174 kb plasmid, known as pMUM001...
  3. pmc Evolution of two distinct phylogenetic lineages of the emerging human pathogen Mycobacterium ulcerans
    Michael Kaser
    Swiss Tropical Institute, Socinstr, 57, 4002 Basel, Switzerland
    BMC Evol Biol 7:177. 2007
    ..ulcerans, to define features of a hypothetical M. ulcerans most recent common ancestor and to confirm its origin from Mycobacterium marinum...
  4. ncbi request reprint Functional analysis and annotation of the virulence plasmid pMUM001 from Mycobacterium ulcerans
    Timothy P Stinear
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, 28 rue du Docteur Roux, 75725 Paris Cedex 15, France
    Microbiology 151:683-92. 2005
    ..The results presented here provide important insights into this unusual virulence plasmid from an emerging but neglected human pathogen...
  5. pmc Common evolutionary origin for the unstable virulence plasmid pMUM found in geographically diverse strains of Mycobacterium ulcerans
    Timothy P Stinear
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, Paris, France
    J Bacteriol 187:1668-76. 2005
    ..Our results suggest that pMUM is a defining characteristic of M. ulcerans but that in the absence of purifying selection, deletion of plasmid sequences and a corresponding loss of mycolactone production readily arise...
  6. pmc Comparative genetic analysis of Mycobacterium ulcerans and Mycobacterium marinum reveals evidence of recent divergence
    T P Stinear
    Bacterial Pathogenesis Research Group, Department of Microbiology, Monash University, Clayton, Victoria, Australia
    J Bacteriol 182:6322-30. 2000
    ..marinum by the acquisition and concomitant loss of DNA in a manner analogous to the emergence of M. tuberculosis, where species diversity is being driven mainly by the activity of mobile DNA elements...
  7. pmc A simple PCR method for rapid genotype analysis of Mycobacterium ulcerans
    T Stinear
    Department of Microbiology, Monash University, Victoria, Australia
    J Clin Microbiol 38:1482-7. 2000
    ....
  8. pmc Identification and characterization of IS2404 and IS2606: two distinct repeated sequences for detection of Mycobacterium ulcerans by PCR
    T Stinear
    Department of Microbiology, Monash University, Clayton, Victoria, Australia
    J Clin Microbiol 37:1018-23. 1999
    ..ulcerans among large numbers of other environmental mycobacteria...
  9. pmc Insights from the complete genome sequence of Mycobacterium marinum on the evolution of Mycobacterium tuberculosis
    Timothy P Stinear
    Department of Microbiology, Monash University, Clayton 3800, Australia
    Genome Res 18:729-41. 2008
    ..tuberculosis. The work described herein provides a foundation for using M. marinum to better understand the determinants of pathogenesis of tuberculosis...
  10. pmc Detection of Mycobacterium ulcerans in environmental samples during an outbreak of ulcerative disease
    B C Ross
    Department of Microbiology and Infectious Disease, Royal Children s Hospital, Melbourne, Victoria, Australia
    Appl Environ Microbiol 63:4135-8. 1997
    ..This is the first time that M. ulcerans has been demonstrated to be present in the environment and supports the postulated epidemiology of disease due to this organism...
  11. pmc Identification of Mycobacterium ulcerans in the environment from regions in Southeast Australia in which it is endemic with sequence capture-PCR
    T Stinear
    Department of Microbiology, Monash University, Clayton, Victoria, Australia
    Appl Environ Microbiol 66:3206-13. 2000
    ..ulcerans may be transmitted from a point environmental source and demonstrate the utility of magnetic bead sequence capture-PCR for identification of nonculturable microbial pathogens in the environment...
  12. pmc Two novel point mutations in clinical Staphylococcus aureus reduce linezolid susceptibility and switch on the stringent response to promote persistent infection
    Wei Gao
    Department of Microbiology, Monash University, Clayton, Victoria, Australia
    PLoS Pathog 6:e1000944. 2010
    ..These results reinforce the exquisite adaptability of S. aureus and show how subtle molecular changes cause major alterations in bacterial behaviour, as well as highlighting potential weaknesses of current antibiotic treatment regimens...
  13. pmc Genomic analysis reveals a point mutation in the two-component sensor gene graS that leads to intermediate vancomycin resistance in clinical Staphylococcus aureus
    Benjamin P Howden
    Department of Microbiology, Monash University, Wellington Rd, Clayton, Victoria, Australia
    Antimicrob Agents Chemother 52:3755-62. 2008
    ..However, additional mutations are likely required to express the full VISA phenotype...
  14. doi request reprint Transfer, stable maintenance and expression of the mycolactone polyketide megasynthase mls genes in a recombination-impaired Mycobacterium marinum
    Jessica L Porter
    Department of Microbiology, Monash University, Clayton 3800, Victoria, Australia
    Microbiology 155:1923-33. 2009
    ..These experiments show that it is possible to successfully manipulate and stably transfer the large mls genes, but that other bacterial host factors appear to be required to facilitate mycolactone production...
  15. doi request reprint A sustained hospital outbreak of vancomycin-resistant Enterococcus faecium bacteremia due to emergence of vanB E. faecium sequence type 203
    Paul D R Johnson
    Department of Infectious Diseases and Microbiology, Austin Health, Monash University, Melbourne, Victoria, Australia
    J Infect Dis 202:1278-86. 2010
    ..A significant increase in the rate of vancomycin-resistant Enterococcus faecium (VREfm) bacteremia at our health service, despite improved infection control, prompted us to investigate the cause...
  16. pmc Reductive evolution and niche adaptation inferred from the genome of Mycobacterium ulcerans, the causative agent of Buruli ulcer
    Timothy P Stinear
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, 75725 Paris Cedex 15, France
    Genome Res 17:192-200. 2007
    ..ulcerans and M. marinum. This work offers significant insight into the biology and evolution of mycobacterial pathogens and is an important component of international efforts to counter Buruli ulcer...
  17. pmc Development and application of two multiplex real-time PCR assays for the detection of Mycobacterium ulcerans in clinical and environmental samples
    Janet A M Fyfe
    Victorian Infectious Diseases Reference Laboratory, North Melbourne, Victoria, Australia
    Appl Environ Microbiol 73:4733-40. 2007
    ..This suite of new methods is enabling rapid progress in the understanding of the ecology of this important human pathogen...
  18. pmc A major role for mammals in the ecology of Mycobacterium ulcerans
    Janet A M Fyfe
    Victorian Infectious Diseases Reference Laboratory, North Melbourne, Victoria, Australia
    PLoS Negl Trop Dis 4:e791. 2010
    ..The purpose of this study was to investigate the environmental distribution of M. ulcerans in south-eastern Australia...
  19. pmc Mycolactone gene expression is controlled by strong SigA-like promoters with utility in studies of Mycobacterium ulcerans and buruli ulcer
    Nicholas J Tobias
    Department of Microbiology, Monash University, Clayton, Victoria, Australia
    PLoS Negl Trop Dis 3:e553. 2009
    ..ulcerans-GFP, a strain in which fluorescence and toxin gene expression are linked, thus providing a tool for studying Buruli ulcer pathogenesis and potential transmission to humans...
  20. pmc Isolates with low-level vancomycin resistance associated with persistent methicillin-resistant Staphylococcus aureus bacteremia
    Benjamin P Howden
    Department of Microbiology, Monash University, Clayton, 3800 Victoria, Australia
    Antimicrob Agents Chemother 50:3039-47. 2006
    ..These clinically derived pairs of isolates will be a useful resource to elucidate the genetic mechanism of resistance in hVISA/VISA strains...
  21. pmc Detection of a single viable Cryptosporidium parvum oocyst in environmental water concentrates by reverse transcription-PCR
    T Stinear
    WATER ECOscience Pty Ltd, Mount Waverley, Victoria, Australia
    Appl Environ Microbiol 62:3385-90. 1996
    ..The technique is versatile, straightforward, and rapid (1 day) and provides a sensitive and economic means of screening concentrated water samples for the presence of C. parvum...
  22. pmc Reduced vancomycin susceptibility in Staphylococcus aureus, including vancomycin-intermediate and heterogeneous vancomycin-intermediate strains: resistance mechanisms, laboratory detection, and clinical implications
    Benjamin P Howden
    Department of Infectious Diseases, Austin Health, Victoria, Australia
    Clin Microbiol Rev 23:99-139. 2010
    ..Given the subtleties of vancomycin susceptibility testing against S. aureus, it is imperative that diagnostic laboratories use well-standardized methods and have a framework for detecting reduced vancomycin susceptibility in S. aureus...
  23. pmc Giant plasmid-encoded polyketide synthases produce the macrolide toxin of Mycobacterium ulcerans
    Timothy P Stinear
    Unite de Genetique Moleculaire Bacterienne, Genopole, Institut Pasteur, 28 rue du Docteur Roux, 75725 Paris Cedex 15, France
    Proc Natl Acad Sci U S A 101:1345-9. 2004
    ..Such detailed knowledge of mycolactone will further the investigation of its mode of action and the development of urgently needed therapeutic strategies to combat Buruli ulcer...
  24. doi request reprint Comprehensive proteome analysis of Mycobacterium ulcerans and quantitative comparison of mycolactone biosynthesis
    Petra Tafelmeyer
    Plate forme de Protéomique, Institut Pasteur, Paris, France
    Proteomics 8:3124-38. 2008
    ....
  25. pmc Sensitive and rapid detection of viable Giardia cysts and Cryptosporidium parvum oocysts in large-volume water samples with wound fiberglass cartridge filters and reverse transcription-PCR
    C Kaucner
    WATER ECOscience Pty Ltd, Mount Waverley, Victoria, Australia
    Appl Environ Microbiol 64:1743-9. 1998
    ..This combination of the large-volume sampling method with RT-PCR represents a significant advance in terms of protozoan pathogen monitoring and in the wider application of PCR technology to this field of microbiology...
  26. pmc Complete genome sequence of Staphylococcus aureus strain JKD6159, a unique Australian clone of ST93-IV community methicillin-resistant Staphylococcus aureus
    Kyra Chua
    Department of Microbiology, Monash University, Melbourne, Victoria, Australia
    J Bacteriol 192:5556-7. 2010
    ..This isolate is a representative of the most common Australian clone of cMRSA that is more distantly related to the previously sequenced genomes of S. aureus...
  27. pmc Mycobacterium ulcerans in mosquitoes captured during outbreak of Buruli ulcer, southeastern Australia
    Paul D R Johnson
    Infectious Disease Department, Austin Health, Melbourne, Victoria, Australia
    Emerg Infect Dis 13:1653-60. 2007
    ..Forty-eight pools (5 species) were positive for insertion sequence IS2404 (maximum likelihood estimate 4.3/1,000), and we confirmed the presence of M. ulcerans in a subset of pools by detection of 3 additional PCR targets...
  28. doi request reprint Evaluation of VNTR typing for the identification of Mycobacterium ulcerans in environmental samples from Victoria, Australia
    Caroline J Lavender
    Mycobacterium Reference Laboratory, Victorian Infectious Diseases Reference Laboratory, 10 Wreckyn Street, North Melbourne, Victoria, Australia
    FEMS Microbiol Lett 287:250-5. 2008
    ..ulcerans being detected was indistinguishable from the strain causing disease in humans in that region...
  29. pmc Subtractive hybridization reveals a type I polyketide synthase locus specific to Mycobacterium ulcerans
    Grant A Jenkin
    Bacterial Pathogenesis Research Group, Department of Microbiology, Monash University, Clayton, Victoria, Australia
    J Bacteriol 185:6870-82. 2003
    ..ulcerans, in particular, a type I polyketide synthase locus with a highly repetitive modular arrangement. We postulate that this locus is responsible for the synthesis of mycolactone in M. ulcerans...
  30. doi request reprint Buruli ulcer: reductive evolution enhances pathogenicity of Mycobacterium ulcerans
    Caroline Demangel
    Institut Pasteur, UP Pathogénomique Mycobactérienne Intégrée, Paris, France
    Nat Rev Microbiol 7:50-60. 2009
    ..Here, the ecology, microbiology, evolutionary genomics and immunopathology of Buruli ulcer are reviewed...
  31. ncbi request reprint First case of Mycobacterium ulcerans disease (Bairnsdale or Buruli ulcer) acquired in New South Wales
    Caroline J Lavender
    Mycobacterium Reference Laboratory, Victorian Infectious Diseases Reference Laboratory, Melbourne, Victoria, Australia
    Med J Aust 186:62-3. 2007
    ..To our knowledge, this is the first case of M. ulcerans infection acquired in NSW...