Research Topics
| T StinearSummaryAffiliation: Monash University Country: Australia Publications
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Publications
Different bacterial gene expression patterns and attenuated host immune responses are associated with the evolution of low-level vancomycin resistance during persistent methicillin-resistant Staphylococcus aureus bacteraemiaBenjamin P Howden
Australian Bacterial Pathogenesis Program, Department of Microbiology, Monash University, Clayton, Victoria, Australia
BMC Microbiol 8:39. 2008..Based on the transcriptome results multiple genes were sequenced and innate immune system stimulation was assessed in the VSSA and hVISA/VISA pairs...- Deciphering the genetic basis for polyketide variation among mycobacteria producing mycolactonesSacha J Pidot
Department of Microbiology, Monash University, Clayton, 3800, Australia
BMC Genomics 9:462. 2008..This work has shed light on the evolution of mycolactone biosynthesis among an unusual group of mycobacteria and highlights the potential of the mls locus to become a toolbox for combinatorial PKS biochemistry... - Evolution of two distinct phylogenetic lineages of the emerging human pathogen Mycobacterium ulceransMichael Kaser
Swiss Tropical Institute, Socinstr, 57, 4002 Basel, Switzerland
BMC Evol Biol 7:177. 2007..ulcerans, to define features of a hypothetical M. ulcerans most recent common ancestor and to confirm its origin from Mycobacterium marinum... - Functional analysis and annotation of the virulence plasmid pMUM001 from Mycobacterium ulceransTimothy P Stinear
Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, 28 rue du Docteur Roux, 75725 Paris Cedex 15, France
Microbiology 151:683-92. 2005..The results presented here provide important insights into this unusual virulence plasmid from an emerging but neglected human pathogen... - Common evolutionary origin for the unstable virulence plasmid pMUM found in geographically diverse strains of Mycobacterium ulceransTimothy P Stinear
Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, Paris, France
J Bacteriol 187:1668-76. 2005..Our results suggest that pMUM is a defining characteristic of M. ulcerans but that in the absence of purifying selection, deletion of plasmid sequences and a corresponding loss of mycolactone production readily arise... - Comparative genetic analysis of Mycobacterium ulcerans and Mycobacterium marinum reveals evidence of recent divergenceT P Stinear
Bacterial Pathogenesis Research Group, Department of Microbiology, Monash University, Clayton, Victoria, Australia
J Bacteriol 182:6322-30. 2000..marinum by the acquisition and concomitant loss of DNA in a manner analogous to the emergence of M. tuberculosis, where species diversity is being driven mainly by the activity of mobile DNA elements... - A simple PCR method for rapid genotype analysis of Mycobacterium ulceransT Stinear
Department of Microbiology, Monash University, Victoria, Australia
J Clin Microbiol 38:1482-7. 2000.... - Identification and characterization of IS2404 and IS2606: two distinct repeated sequences for detection of Mycobacterium ulcerans by PCRT Stinear
Department of Microbiology, Monash University, Clayton, Victoria, Australia
J Clin Microbiol 37:1018-23. 1999..ulcerans among large numbers of other environmental mycobacteria... - Insights from the complete genome sequence of Mycobacterium marinum on the evolution of Mycobacterium tuberculosisTimothy P Stinear
Department of Microbiology, Monash University, Clayton 3800, Australia
Genome Res 18:729-41. 2008..tuberculosis. The work described herein provides a foundation for using M. marinum to better understand the determinants of pathogenesis of tuberculosis... - Detection of Mycobacterium ulcerans in environmental samples during an outbreak of ulcerative diseaseB C Ross
Department of Microbiology and Infectious Disease, Royal Children s Hospital, Melbourne, Victoria, Australia
Appl Environ Microbiol 63:4135-8. 1997..This is the first time that M. ulcerans has been demonstrated to be present in the environment and supports the postulated epidemiology of disease due to this organism... - Identification of Mycobacterium ulcerans in the environment from regions in Southeast Australia in which it is endemic with sequence capture-PCRT Stinear
Department of Microbiology, Monash University, Clayton, Victoria, Australia
Appl Environ Microbiol 66:3206-13. 2000..ulcerans may be transmitted from a point environmental source and demonstrate the utility of magnetic bead sequence capture-PCR for identification of nonculturable microbial pathogens in the environment... - Two novel point mutations in clinical Staphylococcus aureus reduce linezolid susceptibility and switch on the stringent response to promote persistent infectionWei Gao
Department of Microbiology, Monash University, Clayton, Victoria, Australia
PLoS Pathog 6:e1000944. 2010..These results reinforce the exquisite adaptability of S. aureus and show how subtle molecular changes cause major alterations in bacterial behaviour, as well as highlighting potential weaknesses of current antibiotic treatment regimens... - Genomic analysis reveals a point mutation in the two-component sensor gene graS that leads to intermediate vancomycin resistance in clinical Staphylococcus aureusBenjamin P Howden
Department of Microbiology, Monash University, Wellington Rd, Clayton, Victoria, Australia
Antimicrob Agents Chemother 52:3755-62. 2008..However, additional mutations are likely required to express the full VISA phenotype... - Transfer, stable maintenance and expression of the mycolactone polyketide megasynthase mls genes in a recombination-impaired Mycobacterium marinumJessica L Porter
Department of Microbiology, Monash University, Clayton 3800, Victoria, Australia
Microbiology 155:1923-33. 2009..These experiments show that it is possible to successfully manipulate and stably transfer the large mls genes, but that other bacterial host factors appear to be required to facilitate mycolactone production... - A sustained hospital outbreak of vancomycin-resistant Enterococcus faecium bacteremia due to emergence of vanB E. faecium sequence type 203Paul D R Johnson
Department of Infectious Diseases and Microbiology, Austin Health, Monash University, Melbourne, Victoria, Australia
J Infect Dis 202:1278-86. 2010..A significant increase in the rate of vancomycin-resistant Enterococcus faecium (VREfm) bacteremia at our health service, despite improved infection control, prompted us to investigate the cause... - Reductive evolution and niche adaptation inferred from the genome of Mycobacterium ulcerans, the causative agent of Buruli ulcerTimothy P Stinear
Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, 75725 Paris Cedex 15, France
Genome Res 17:192-200. 2007..ulcerans and M. marinum. This work offers significant insight into the biology and evolution of mycobacterial pathogens and is an important component of international efforts to counter Buruli ulcer... - Development and application of two multiplex real-time PCR assays for the detection of Mycobacterium ulcerans in clinical and environmental samplesJanet A M Fyfe
Victorian Infectious Diseases Reference Laboratory, North Melbourne, Victoria, Australia
Appl Environ Microbiol 73:4733-40. 2007..This suite of new methods is enabling rapid progress in the understanding of the ecology of this important human pathogen... - A major role for mammals in the ecology of Mycobacterium ulceransJanet A M Fyfe
Victorian Infectious Diseases Reference Laboratory, North Melbourne, Victoria, Australia
PLoS Negl Trop Dis 4:e791. 2010..The purpose of this study was to investigate the environmental distribution of M. ulcerans in south-eastern Australia... - Mycolactone gene expression is controlled by strong SigA-like promoters with utility in studies of Mycobacterium ulcerans and buruli ulcerNicholas J Tobias
Department of Microbiology, Monash University, Clayton, Victoria, Australia
PLoS Negl Trop Dis 3:e553. 2009..ulcerans-GFP, a strain in which fluorescence and toxin gene expression are linked, thus providing a tool for studying Buruli ulcer pathogenesis and potential transmission to humans... - Isolates with low-level vancomycin resistance associated with persistent methicillin-resistant Staphylococcus aureus bacteremiaBenjamin P Howden
Department of Microbiology, Monash University, Clayton, 3800 Victoria, Australia
Antimicrob Agents Chemother 50:3039-47. 2006..These clinically derived pairs of isolates will be a useful resource to elucidate the genetic mechanism of resistance in hVISA/VISA strains... - Detection of a single viable Cryptosporidium parvum oocyst in environmental water concentrates by reverse transcription-PCRT Stinear
WATER ECOscience Pty Ltd, Mount Waverley, Victoria, Australia
Appl Environ Microbiol 62:3385-90. 1996..The technique is versatile, straightforward, and rapid (1 day) and provides a sensitive and economic means of screening concentrated water samples for the presence of C. parvum... - Reduced vancomycin susceptibility in Staphylococcus aureus, including vancomycin-intermediate and heterogeneous vancomycin-intermediate strains: resistance mechanisms, laboratory detection, and clinical implicationsBenjamin P Howden
Department of Infectious Diseases, Austin Health, Victoria, Australia
Clin Microbiol Rev 23:99-139. 2010..Given the subtleties of vancomycin susceptibility testing against S. aureus, it is imperative that diagnostic laboratories use well-standardized methods and have a framework for detecting reduced vancomycin susceptibility in S. aureus... - Giant plasmid-encoded polyketide synthases produce the macrolide toxin of Mycobacterium ulceransTimothy P Stinear
, , Institut Pasteur, 28 rue du Docteur Roux, 75725 Paris Cedex 15, France
Proc Natl Acad Sci U S A 101:1345-9. 2004..Such detailed knowledge of mycolactone will further the investigation of its mode of action and the development of urgently needed therapeutic strategies to combat Buruli ulcer... - Comprehensive proteome analysis of Mycobacterium ulcerans and quantitative comparison of mycolactone biosynthesisPetra Tafelmeyer
Plate forme de Protéomique, Institut Pasteur, Paris, France
Proteomics 8:3124-38. 2008.... - Sensitive and rapid detection of viable Giardia cysts and Cryptosporidium parvum oocysts in large-volume water samples with wound fiberglass cartridge filters and reverse transcription-PCRC Kaucner
WATER ECOscience Pty Ltd, Mount Waverley, Victoria, Australia
Appl Environ Microbiol 64:1743-9. 1998..This combination of the large-volume sampling method with RT-PCR represents a significant advance in terms of protozoan pathogen monitoring and in the wider application of PCR technology to this field of microbiology... - Complete genome sequence of Staphylococcus aureus strain JKD6159, a unique Australian clone of ST93-IV community methicillin-resistant Staphylococcus aureusKyra Chua
Department of Microbiology, Monash University, Melbourne, Victoria, Australia
J Bacteriol 192:5556-7. 2010..This isolate is a representative of the most common Australian clone of cMRSA that is more distantly related to the previously sequenced genomes of S. aureus... - Mycobacterium ulcerans in mosquitoes captured during outbreak of Buruli ulcer, southeastern AustraliaPaul D R Johnson
Infectious Disease Department, Austin Health, Melbourne, Victoria, Australia
Emerg Infect Dis 13:1653-60. 2007..Forty-eight pools (5 species) were positive for insertion sequence IS2404 (maximum likelihood estimate 4.3/1,000), and we confirmed the presence of M. ulcerans in a subset of pools by detection of 3 additional PCR targets... - Evaluation of VNTR typing for the identification of Mycobacterium ulcerans in environmental samples from Victoria, AustraliaCaroline J Lavender
Mycobacterium Reference Laboratory, Victorian Infectious Diseases Reference Laboratory, 10 Wreckyn Street, North Melbourne, Victoria, Australia
FEMS Microbiol Lett 287:250-5. 2008..ulcerans being detected was indistinguishable from the strain causing disease in humans in that region... - Subtractive hybridization reveals a type I polyketide synthase locus specific to Mycobacterium ulceransGrant A Jenkin
Bacterial Pathogenesis Research Group, Department of Microbiology, Monash University, Clayton, Victoria, Australia
J Bacteriol 185:6870-82. 2003..ulcerans, in particular, a type I polyketide synthase locus with a highly repetitive modular arrangement. We postulate that this locus is responsible for the synthesis of mycolactone in M. ulcerans... - Buruli ulcer: reductive evolution enhances pathogenicity of Mycobacterium ulceransCaroline Demangel
Institut Pasteur, UP Pathogénomique Mycobactérienne Intégrée, Paris, France
Nat Rev Microbiol 7:50-60. 2009..Here, the ecology, microbiology, evolutionary genomics and immunopathology of Buruli ulcer are reviewed... - First case of Mycobacterium ulcerans disease (Bairnsdale or Buruli ulcer) acquired in New South WalesCaroline J Lavender
Mycobacterium Reference Laboratory, Victorian Infectious Diseases Reference Laboratory, Melbourne, Victoria, Australia
Med J Aust 186:62-3. 2007..To our knowledge, this is the first case of M. ulcerans infection acquired in NSW...