Michael T Ryan
Affiliation: Monash University
Kamerkar S, Kraus F, Sharpe A, Pucadyil T, Ryan M. Dynamin-related protein 1 has membrane constricting and severing abilities sufficient for mitochondrial and peroxisomal fission. Nat Commun. 2018;9:5239 pubmed publisher
..We conclude that Drp1 has both membrane constricting and severing abilities and is the dominant dynamin performing mitochondrial and peroxisomal fission. ..
Formosa L, Hofer A, Tischner C, Wenz T, Ryan M. Translation and Assembly of Radiolabeled Mitochondrial DNA-Encoded Protein Subunits from Cultured Cells and Isolated Mitochondria. Methods Mol Biol. 2016;1351:115-29 pubmed publisher
Osellame L, Singh A, Stroud D, Palmer C, Stojanovski D, Ramachandran R, et al
. Cooperative and independent roles of the Drp1 adaptors Mff, MiD49 and MiD51 in mitochondrial fission. J Cell Sci. 2016;129:2170-81 pubmed publisher
..Furthermore, we found that MiD51 can suppress Mff-dependent enhancement of Drp1 GTPase activity. Our data indicates that Mff and MiD51 regulate Drp1 in specific ways to promote mitochondrial fission. ..
Kraus F, Ryan M. The constriction and scission machineries involved in mitochondrial fission. J Cell Sci. 2017;130:2953-2960 pubmed publisher
Formosa L, Mimaki M, Frazier A, McKenzie M, Stait T, Thorburn D, et al
. Characterization of mitochondrial FOXRED1 in the assembly of respiratory chain complex I. Hum Mol Genet. 2015;24:2952-65 pubmed publisher
..Our studies reveal that FOXRED1 is a crucial component in the productive assembly of complex I and that mutations in FOXRED1 leading to partial loss of function cause defects in complex I biogenesis. ..
Richter V, Singh A, Kvansakul M, Ryan M, Osellame L. Splitting up the powerhouse: structural insights into the mechanism of mitochondrial fission. Cell Mol Life Sci. 2015;72:3695-707 pubmed publisher
..This review focuses on our current understanding of mitochondrial fission and the proteins that regulate this process in cell homeostasis, with a particular focus on the recent mechanistic insights based on protein structures. ..
Stroud D, Maher M, Lindau C, VÃ¶gtle F, Frazier A, Surgenor E, et al
. COA6 is a mitochondrial complex IV assembly factor critical for biogenesis of mtDNA-encoded COX2. Hum Mol Genet. 2015;24:5404-15 pubmed publisher
..Our data reveal that COA6 is intricately involved in the copper-dependent biogenesis of COX2. ..
Formosa L, Dibley M, Stroud D, Ryan M. Building a complex complex: Assembly of mitochondrial respiratory chain complex I. Semin Cell Dev Biol. 2018;76:154-162 pubmed publisher
..This review outlines the current understanding of complex I biogenesis, with an emphasis on the assembly factors that facilitate the building of this architectural giant. ..