J I Rood

Summary

Affiliation: Monash University
Country: Australia

Publications

  1. ncbi request reprint Virulence genes of Clostridium perfringens
    J I Rood
    Department of Microbiology, Monash University, Clayton, Australia
    Annu Rev Microbiol 52:333-60. 1998
  2. pmc Programmed cellular necrosis mediated by the pore-forming alpha-toxin from Clostridium septicum
    Catherine L Kennedy
    Australian Bacterial Pathogenesis Research Program, Department of Microbiology, Monash University, Clayton, Victoria, Australia
    PLoS Pathog 5:e1000516. 2009
  3. pmc Toxin B is essential for virulence of Clostridium difficile
    Dena Lyras
    Australian Bacterial Pathogenesis Program, Department of Microbiology, Monash University, Victoria 3800, Australia
    Nature 458:1176-9. 2009
  4. pmc Functional analysis of the VirSR phosphorelay from Clostridium perfringens
    Jackie K Cheung
    Department of Microbiology, Monash University, Clayton, Victoria, Australia
    PLoS ONE 4:e5849. 2009
  5. ncbi request reprint Comparison of gene probe and conventional methods for the differentiation of ovine footrot isolates of Dichelobacter nodosus
    J I Rood
    Department of Microbiology, Monash University, Clayton, Australia
    Vet Microbiol 52:127-41. 1996
  6. pmc Functional identification of conjugation and replication regions of the tetracycline resistance plasmid pCW3 from Clostridium perfringens
    Trudi L Bannam
    Australian Research Council Centre of Excellence in Structural and Functional Microbial genomics and Victorian Bioinformatics Consortium, Department of Microbiology, Monash University, Victoria 3800, Australia
    J Bacteriol 188:4942-51. 2006
  7. ncbi request reprint Electroporation-mediated transformation of the ovine footrot pathogen Dichelobacter nodosus
    R M Kennan
    Department of Microbiology, Monash University, Clayton, Vic, Australia
    FEMS Microbiol Lett 169:383-9. 1998
  8. ncbi request reprint The Clostridium perfringens Tet P determinant comprises two overlapping genes: tetA(P), which mediates active tetracycline efflux, and tetB(P), which is related to the ribosomal protection family of tetracycline-resistance determinants
    J Sloan
    Department of Microbiology, Monash University, Clayton, Australia
    Mol Microbiol 11:403-15. 1994
  9. ncbi request reprint Transposition of Tn4451 and Tn4453 involves a circular intermediate that forms a promoter for the large resolvase, TnpX
    D Lyras
    Bacterial Pathogenesis Research Group, Department of Microbiology, PO Box 53, Monash University, Victoria 3800, Australia
    Mol Microbiol 38:588-601. 2000
  10. pmc Chloramphenicol resistance in Clostridium difficile is encoded on Tn4453 transposons that are closely related to Tn4451 from Clostridium perfringens
    D Lyras
    Department of Microbiology, Monash University, Clayton, Victoria, Australia
    Antimicrob Agents Chemother 42:1563-7. 1998

Collaborators

Detail Information

Publications72

  1. ncbi request reprint Virulence genes of Clostridium perfringens
    J I Rood
    Department of Microbiology, Monash University, Clayton, Australia
    Annu Rev Microbiol 52:333-60. 1998
    ..The production of many of the extracellular toxins is regulated at the transcriptional level by the products of the virR and virS genes, which together comprise a two-component signal transduction system...
  2. pmc Programmed cellular necrosis mediated by the pore-forming alpha-toxin from Clostridium septicum
    Catherine L Kennedy
    Australian Bacterial Pathogenesis Research Program, Department of Microbiology, Monash University, Clayton, Victoria, Australia
    PLoS Pathog 5:e1000516. 2009
    ..septicum-mediated myonecrosis and sepsis. We postulate that cellular intoxication with pore-forming toxins may be a major mechanism by which programmed necrosis is induced...
  3. pmc Toxin B is essential for virulence of Clostridium difficile
    Dena Lyras
    Australian Bacterial Pathogenesis Program, Department of Microbiology, Monash University, Victoria 3800, Australia
    Nature 458:1176-9. 2009
    ....
  4. pmc Functional analysis of the VirSR phosphorelay from Clostridium perfringens
    Jackie K Cheung
    Department of Microbiology, Monash University, Clayton, Victoria, Australia
    PLoS ONE 4:e5849. 2009
    ..These results provide good evidence that phosphorylation is critical for VirR function...
  5. ncbi request reprint Comparison of gene probe and conventional methods for the differentiation of ovine footrot isolates of Dichelobacter nodosus
    J I Rood
    Department of Microbiology, Monash University, Clayton, Australia
    Vet Microbiol 52:127-41. 1996
    ..It is suggested that diagnostic laboratories consider carrying out both this PCR test and tests based on the extracellular proteases of D. nodosus...
  6. pmc Functional identification of conjugation and replication regions of the tetracycline resistance plasmid pCW3 from Clostridium perfringens
    Trudi L Bannam
    Australian Research Council Centre of Excellence in Structural and Functional Microbial genomics and Victorian Bioinformatics Consortium, Department of Microbiology, Monash University, Victoria 3800, Australia
    J Bacteriol 188:4942-51. 2006
    ....
  7. ncbi request reprint Electroporation-mediated transformation of the ovine footrot pathogen Dichelobacter nodosus
    R M Kennan
    Department of Microbiology, Monash University, Clayton, Vic, Australia
    FEMS Microbiol Lett 169:383-9. 1998
    ..nodosus and for footrot pathogenesis studies, since by using reverse genetics it will now be possible to examine the role of putative D. nodosus-encoded virulence genes in the disease process...
  8. ncbi request reprint The Clostridium perfringens Tet P determinant comprises two overlapping genes: tetA(P), which mediates active tetracycline efflux, and tetB(P), which is related to the ribosomal protection family of tetracycline-resistance determinants
    J Sloan
    Department of Microbiology, Monash University, Clayton, Australia
    Mol Microbiol 11:403-15. 1994
    ..It is proposed that tetB(P) evolved from the conjugative transfer into C. perfringens of a tet(M)-like gene from another bacterium...
  9. ncbi request reprint Transposition of Tn4451 and Tn4453 involves a circular intermediate that forms a promoter for the large resolvase, TnpX
    D Lyras
    Bacterial Pathogenesis Research Group, Department of Microbiology, PO Box 53, Monash University, Victoria 3800, Australia
    Mol Microbiol 38:588-601. 2000
    ....
  10. pmc Chloramphenicol resistance in Clostridium difficile is encoded on Tn4453 transposons that are closely related to Tn4451 from Clostridium perfringens
    D Lyras
    Department of Microbiology, Monash University, Clayton, Victoria, Australia
    Antimicrob Agents Chemother 42:1563-7. 1998
    ..difficile and C. perfringens has implications for the evolution and dissemination of antibiotic resistance genes and the mobile elements on which they are found within the clostridia...
  11. ncbi request reprint Construction of a sequenced Clostridium perfringens-Escherichia coli shuttle plasmid
    J Sloan
    Department of Microbiology, Monash University, Clayton, Australia
    Plasmid 27:207-19. 1992
    ..The versatility of pJIR418 and its applicability for the cloning of toxin genes from C. perfringens have been demonstrated by the manipulation of a cloned gene encoding the production of phospholipase C...
  12. ncbi request reprint Induction of pCW3-encoded tetracycline resistance in Clostridium perfringens involves a host-encoded factor
    P A Johanesen
    Bacterial Pathogenesis Research Group, Monash University, Victoria 3800, Australia
    Plasmid 46:229-32. 2001
    ..Based on these results it is proposed that induction of the pCW3-encoded tet(P) genes in C. perfringens requires a host-encoded factor that is either absent or nonfunctional in strain 13 derivatives...
  13. pmc Glutamate residues located within putative transmembrane helices are essential for TetA(P)-mediated tetracycline efflux
    R M Kennan
    Department of Microbiology, Monash University, Clayton, Australia
    J Bacteriol 179:7011-5. 1997
    ..It is concluded that negatively charged residues that appear to be located within or near the membrane are important for the function of TetA(P)...
  14. pmc The type IV fimbrial subunit gene (fimA) of Dichelobacter nodosus is essential for virulence, protease secretion, and natural competence
    R M Kennan
    Bacterial Pathogenesis Research Group, Department of Microbiology, Monash University, Victoria 3800, Australia
    J Bacteriol 183:4451-8. 2001
    ..nodosus virulence gene. These studies represent the first time that molecular genetics has been used to determine the role of virulence genes in this slow growing anaerobic bacterium...
  15. pmc Genetic organization and distribution of tetracycline resistance determinants in Clostridium perfringens
    D Lyras
    Department of Microbiology, Monash University, Clayton, Victoria, Australia
    Antimicrob Agents Chemother 40:2500-4. 1996
    ..Nucleotide sequence analysis of isolates lacking tetB(P) revealed that they contained the tetA408(P) gene, which lacked the codons for the 12 carboxy-terminal amino acids of the TetA(P) protein...
  16. ncbi request reprint Genomic analysis of the erythromycin resistance element Tn5398 from Clostridium difficile
    K A Farrow
    Bacterial Pathogenesis Research Group, Department of Microbiology, PO Box 53, Monash University, Victoria 3800, Australia
    Microbiology 147:2717-28. 2001
    ..These results indicate that genetic exchange and recombination between these determinants occurs in the clinical and natural environment...
  17. pmc Transcriptional analysis of the tet(P) operon from Clostridium perfringens
    P A Johanesen
    Bacterial Pathogenesis Research Group, Department of Microbiology, Monash University, Victoria 3800, Australia
    J Bacteriol 183:7110-9. 2001
    ..It is postulated that the T1 terminator is an intrinsic control element of the tet(P) operon and that it acts to prevent the overexpression of the TetA(P) transmembrane protein, even in the presence of tetracycline...
  18. ncbi request reprint The FxRxHrS motif: a conserved region essential for DNA binding of the VirR response regulator from Clostridium perfringens
    Sheena McGowan
    Bacterial Pathogenesis Research Group, Department of Microbiology, Monash University, 3800 Australia
    J Mol Biol 322:997-1011. 2002
    ..These studies have important implications for this new family of transcriptional factors since they imply that the conserved FxRxHrS motif may be involved in DNA binding in all of these proteins, irrespective of their biological role...
  19. pmc The SKHR motif is required for biological function of the VirR response regulator from Clostridium perfringens
    Sheena McGowan
    Bacterial Pathogenesis Research Group, Department of Microbiology, Monash University, Victoria 3800, Australia
    J Bacteriol 185:6205-8. 2003
    ..Analysis of the mutated proteins indicated that they were unable to bind to the DNA target with the same efficiency as the wild-type protein...
  20. pmc Binary toxin production in Clostridium difficile is regulated by CdtR, a LytTR family response regulator
    Glen P Carter
    Australian Bacterial Pathogenesis Program, Department of Microbiology, Monash University, Victoria, Australia
    J Bacteriol 189:7290-301. 2007
    ....
  21. ncbi request reprint The alpha-toxin of Clostridium septicum is essential for virulence
    Catherine L Kennedy
    Australian Bacterial Pathogenesis Program, Department of Microbiology, Monash University, VIC 3800, Australia
    Mol Microbiol 57:1357-66. 2005
    ..This study represents the first definitive identification of a virulence factor in this organism and opens the way for further studies that will delineate the role of other putative virulence factors in this significant pathogen...
  22. pmc Synergistic effects of alpha-toxin and perfringolysin O in Clostridium perfringens-mediated gas gangrene
    M M Awad
    Bacterial Pathogenesis Research Group, Department of Microbiology, Monash University, Victoria 3800, Australia
    Infect Immun 69:7904-10. 2001
    ..The results of these virulence experiments clearly support the hypothesis that alpha-toxin and perfringolysin O have a synergistic effect in the pathology of gas gangrene...
  23. ncbi request reprint Construction and analysis of chromosomal Clostridium difficile mutants
    Jennifer R O'Connor
    Australian Bacterial Pathogenesis Program, Department of Microbiology, Monash University, VIC 3800, Australia
    Mol Microbiol 61:1335-51. 2006
    ..The development of this methodology will significantly enhance our ability to use molecular approaches to develop a greater understanding of the ability of C. difficile to cause disease...
  24. pmc The VirSR two-component signal transduction system regulates NetB toxin production in Clostridium perfringens
    Jackie K Cheung
    Department of Microbiology, Monash University, Clayton, Vic, Australia
    Infect Immun 78:3064-72. 2010
    ..We postulate that in the gastrointestinal tract of infected birds, NetB production is upregulated when the population of C. perfringens cells reaches a threshold level that leads to activation of the VirSR system...
  25. ncbi request reprint The recA gene from Clostridium perfringens is induced by methyl methanesulphonate and contains an upstream Cheo box
    J L Johnston
    Department of Microbiology, Monash University, Clayton, Victoria, Australia
    Microbiology 143:885-90. 1997
    ..perfringens recA gene also involves the binding of a LexA-like protein to this site...
  26. ncbi request reprint Identification and molecular analysis of a locus that regulates extracellular toxin production in Clostridium perfringens
    M Lyristis
    Department of Microbiology, Monash University, Clayton, Victoria, Australia
    Mol Microbiol 12:761-77. 1994
    ..A model that described the regulation of extracellular toxin production in C. perfringens was constructed...
  27. ncbi request reprint The clostridial mobilisable transposons
    V Adams
    Bacterial Pathogenesis Research Group, Department of Microbiology, P O Box 53, Monash University, Victoria 3800, Australia
    Cell Mol Life Sci 59:2033-43. 2002
    ....
  28. pmc Use of genetically manipulated strains of Clostridium perfringens reveals that both alpha-toxin and theta-toxin are required for vascular leukostasis to occur in experimental gas gangrene
    D M Ellemor
    Department of Pathology and Immunology, Monash Medical School, Alfred Hospital, Prahran, Victoria 3181, Australia
    Infect Immun 67:4902-7. 1999
    ..Thus, both alpha-toxin and theta-toxin are necessary for the characteristic vascular leukostasis observed in clostridial myonecrosis...
  29. pmc Molecular and cellular basis of microvascular perfusion deficits induced by Clostridium perfringens and Clostridium septicum
    Michael J Hickey
    Centre for Inflammatory Diseases, Monash University Department of Medicine, Monash Medical Centre, Clayton, Victoria, Australia
    PLoS Pathog 4:e1000045. 2008
    ..Since C. septicum induces a similar reduction in microvascular perfusion, it is postulated that this function is central to the pathogenesis of clostridial myonecrosis, irrespective of the causative bacterium...
  30. pmc Twitching motility is essential for virulence in Dichelobacter nodosus
    Xiaoyan Han
    Australian Research Council Centre of Excellence in Structure and Functional Microbial Genomics, Victoria 3800, Australia
    J Bacteriol 190:3323-35. 2008
    ....
  31. ncbi request reprint Two distinct regions of the large serine recombinase TnpX are required for DNA binding and biological function
    Vicki Adams
    Australian Bacterial Pathogenesis Program, Department of Microbiology, Monash University, VIC 3800, Australia
    Mol Microbiol 60:591-601. 2006
    ..In summary, we have shown that TnpX is a complex protein that has multiple intra- and intermolecular interaction sites, providing insight into the structural and functional complexity of this important enzyme family...
  32. pmc Regulation of type IV fimbrial biogenesis in Dichelobacter nodosus
    Dane Parker
    Australian Research Council Centre of Excellence for Structrral and Functional Microbial Genomics and Victorian Bioinformatics Consortium, Department of Microbiology, Monash University, Victoria 3800, Australia
    J Bacteriol 188:4801-11. 2006
    ..nodosus to cause ovine footrot is regulated, as we have shown that the biogenesis of type IV fimbriae in D. nodosus is regulated by a sigma 54-dependent PilR/S system that also indirectly controls protease secretion...
  33. pmc Genome reduction in Leptospira borgpetersenii reflects limited transmission potential
    Dieter M Bulach
    Australian Bacterial Pathogenesis Program, Victorian Bioinformatics Consortium, Department of Microbiology, Monash University, Victoria 3800, Australia
    Proc Natl Acad Sci U S A 103:14560-5. 2006
    ..We conclude that L. borgpetersenii is evolving toward dependence on a strict host-to-host transmission cycle...
  34. pmc Functional characterization and localization of the TcpH conjugation protein from Clostridium perfringens
    Wee Lin Teng
    Department of Microbiology, Australian Research Council Centre of Excellence in Structural and Functional Microbial Genomics, Monash University, Victoria 3800, Australia
    J Bacteriol 190:5075-86. 2008
    ....
  35. pmc The NanI and NanJ sialidases of Clostridium perfringens are not essential for virulence
    Martina Chiarezza
    Bacterial Pathogenesis Research Group and ARC Centre of Excellence in Structural and Functional Microbial Genomics, Department of Microbiology, Monash University, Clayton, Victoria 3800, Australia
    Infect Immun 77:4421-8. 2009
    ..No attenuation of virulence was observed for any of these strains, providing evidence that neither the NanI sialidase nor the NanJ sialidase is essential for virulence...
  36. ncbi request reprint Transformation-mediated serogroup conversion of Dichelobacter nodosus
    Ruth M Kennan
    Department of Microbiology, Bacterial Pathogenesis Research Group, Monash University, P O Box 53, Victoria 3800, Australia
    Vet Microbiol 92:169-78. 2003
    ..These data have significant implications for the use of fimbrial vaccines for the control of ovine footrot and suggest that benign strains of D. nodosus could play an important role as a reservoir of alternative fimbrial antigens...
  37. pmc tISCpe8, an IS1595-family lincomycin resistance element located on a conjugative plasmid in Clostridium perfringens
    Dena Lyras
    Bacterial Pathogenesis Research Group and Australian Research Council Centre of Excellence in Structural and Functional Microbial Genomics, Department of Microbiology, Monash University, Clayton, Victoria 3800, Australia
    J Bacteriol 191:6345-51. 2009
    ..This process likely plays a significant role in bacterial adaptation and evolution...
  38. ncbi request reprint The Clostridium perfringens TetA(P) efflux protein contains a functional variant of the Motif A region found in major facilitator superfamily transport proteins
    Trudi L Bannam
    ARC Centre for Structural and Functional Microbial Genomics, Department of Microbiology, Monash University, Victoria 3800, Australia
    Microbiology 150:127-34. 2004
    ..In addition, it was shown that Glu-117 of the TetA(P) protein, which is predicted to be located in TMD4, is important for resistance although a derivative with an aspartate residue at this position is also functional...
  39. doi request reprint Cross-complementation of Clostridium perfringens PLC and Clostridium septicum alpha-toxin mutants reveals PLC is sufficient to mediate gas gangrene
    Catherine L Kennedy
    Australian Bacterial Pathogenesis Research Program, Monash University, Wellington Rd, Clayton, Victoria 3800, Australia
    Microbes Infect 11:413-8. 2009
    ..septicum background, although it could not induce vascular leukostasis. Conversely, while C. septicum alpha-toxin restored some virulence to a C. perfringens plc mutant, it was less active than in its native background...
  40. ncbi request reprint The large resolvase TnpX is the only transposon-encoded protein required for transposition of the Tn4451/3 family of integrative mobilizable elements
    Dena Lyras
    Australian Bacterial Pathogenesis Programme, Department of Microbiology, Monash University, Victoria 3800, Australia
    Mol Microbiol 51:1787-800. 2004
    ....
  41. pmc The spatial organization of the VirR boxes is critical for VirR-mediated expression of the perfringolysin O gene, pfoA, from Clostridium perfringens
    Jackie K Cheung
    Australian Bacterial Pathogenesis Program, Department of Microbiology, Monash University, Victoria 3800, Australia
    J Bacteriol 186:3321-30. 2004
    ..We propose that these interactions are required for VirR-mediated transcriptional activation...
  42. ncbi request reprint DNA binding properties of TnpX indicate that different synapses are formed in the excision and integration of the Tn4451 family
    Vicki Adams
    Australian Bacterial Pathogenesis Program, Department of Microbiology, Monash University, Victoria 3800, Australia
    Mol Microbiol 53:1195-207. 2004
    ..TnpX is the first large serine recombinase in which differential binding to its transposon and target sites has been demonstrated...
  43. ncbi request reprint Identification of the structural and functional domains of the large serine recombinase TnpX from Clostridium perfringens
    Isabelle S Lucet
    Department of Microbiology, Australian Bacterial Pathogenesis Program, Monash University, Victoria 3800, Australia
    J Biol Chem 280:2503-11. 2005
    ..These data provide evidence that TnpX has separate catalytic, DNA binding, and multimerization domains...
  44. pmc Epsilon-toxin plasmids of Clostridium perfringens type D are conjugative
    Meredith L Hughes
    Australian Research Council Centre of Excellence in Structural and Functional Microbial Genomics, Department of Microbiology, Monash University, Victoria 3800, Australia
    J Bacteriol 189:7531-8. 2007
    ..Our study demonstrated the fluid nature of the toxinotypes and their dependence upon the presence or absence of toxin plasmids, some of which have for the first time been shown to be conjugative...
  45. pmc Type IV fimbrial biogenesis is required for protease secretion and natural transformation in Dichelobacter nodosus
    Xiaoyan Han
    Australian Research Council Centre of Excellence in Structural and Functional Microbial Genomics, Department of Microbiology, Monash University, Victoria 3800, Australia
    J Bacteriol 189:5022-33. 2007
    ....
  46. pmc The putative coupling protein TcpA interacts with other pCW3-encoded proteins to form an essential part of the conjugation complex
    Jennifer A Steen
    Department of Microbiology, Monash University, Clayton, Victoria 3800, Australia
    J Bacteriol 191:2926-33. 2009
    ..Based on these results, we have constructed a model for this unique conjugative transfer apparatus...
  47. pmc Perfringolysin O expression in Clostridium perfringens is independent of the upstream pfoR gene
    Milena M Awad
    Bacterial Pathogenesis Research Group, Department of Microbiology, Monash University, Victoria 3800, Australia
    J Bacteriol 184:2034-8. 2002
    ..Since it was not possible to alter perfringolysin O expression by mutation of pfoR, it was concluded that the pfoR gene product is unlikely to have a role in the regulation of pfoA expression in C. perfringens...
  48. pmc TcpA, an FtsK/SpoIIIE homolog, is essential for transfer of the conjugative plasmid pCW3 in Clostridium perfringens
    Jennifer A Parsons
    Department of Microbiology, Australian Research Council Centre of Excellence in Structural and Functional Microbial Genomics, Monash University, Clayton Campus, Victoria 3800, Australia
    J Bacteriol 189:7782-90. 2007
    ..It is postulated that TcpA is the essential DNA translocase or coupling protein encoded by pCW3 and as such represents a key component of the unique conjugation process in C. perfringens...
  49. doi request reprint Methods for gene cloning and targeted mutagenesis
    Glen P Carter
    Department of Microbiology, Monash University, Clayton, Australia
    Methods Mol Biol 646:183-201. 2010
    ..difficile and most importantly to enable the generation of isogenic mutants in this emerging human pathogen. These methods are essential prerequisites for the effective study of gene function in this important bacterium...
  50. ncbi request reprint Chloramphenicol-resistant Neisseria meningitidis containing catP isolated in Australia
    Tiffany R Shultz
    Department of Microbiology, South Eastern Sydney Area Health Services, The Prince of Wales Hospital, Sydney 2031, Australia
    J Antimicrob Chemother 52:856-9. 2003
    ..Methods for screening for chloramphenicol resistance in meningococci and the in vitro parameters that define this resistance are ill defined...
  51. pmc Ecf, an alternative sigma factor from Neisseria gonorrhoeae, controls expression of msrAB, which encodes methionine sulfoxide reductase
    Ishara C Gunesekere
    Australian Bacterial Pathogenesis Program, Department of Microbiology, Monash University, Clayton, Vic, Australia
    J Bacteriol 188:3463-9. 2006
    ..Western blotting confirmed that the increased level of MsrAB protein is dependent on the presence of Ecf...
  52. pmc Pore-forming activity of alpha-toxin is essential for clostridium septicum-mediated myonecrosis
    Catherine L Kennedy
    Department of Microbiology, Monash University, Clayton, Victoria, Australia
    Infect Immun 77:943-51. 2009
    ....
  53. pmc Association between avian necrotic enteritis and Clostridium perfringens strains expressing NetB toxin
    Anthony L Keyburn
    CSIRO Livestock Industries, Australian Animal Health Laboratory, Geelong, Victoria 3220, Australia
    Vet Res 41:21. 2010
    ..This study provides further evidence that NetB is important in pathogenesis and advances our current understanding of C. perfringens virulence factors in avian necrotic enteritis...
  54. ncbi request reprint Genomic islands of Dichelobacter nodosus
    J I Rood
    Bacterial Pathogenesis Research Group, Department of Microbiology, Monash University, Victoria 3800, Australia
    Curr Top Microbiol Immunol 264:47-60. 2002
  55. pmc Identification of essential residues in the Erm(B) rRNA methyltransferase of Clostridium perfringens
    Kylie A Farrow
    Bacterial Pathogenesis Research Group, Department of Microbiology, Monash University, Clayton, Victoria 3800, Australia
    Antimicrob Agents Chemother 46:1253-61. 2002
    ..These regions of the protein therefore represent potential targets for the rational development of methyltransferase inhibitors...
  56. pmc Comparison of the RpoH-dependent regulon and general stress response in Neisseria gonorrhoeae
    Ishara C Gunesekere
    Australian Bacterial Pathogenesis Program, Department of Microbiology, Monash University, VIC 3800, Australia
    J Bacteriol 188:4769-76. 2006
    ..A total of 13 genes were also down-regulated. From these results we concluded that although RpoH acts as the major regulator of protein homeostasis, N. gonorrhoeae has additional means of responding to temperature stress...
  57. pmc Identification of a Dichelobacter nodosus ferric uptake regulator and determination of its regulatory targets
    Dane Parker
    ARC Centre for Structural and Functional Microbial Genomics and Victorian Bioinformatics Consortium, Department of Microbiology, Monash University, Victoria, Australia
    J Bacteriol 187:366-75. 2005
    ..Down-regulated proteins included a putative manganese superoxide dismutase and ornithine decarboxylase. Based on these data, it is suggested that in D. nodosus the Fur protein functions as a regulator of iron and oxidative metabolism...
  58. pmc Crystallization of the virulent and benign subtilisin-like proteases from the ovine footrot pathogen Dichelobacter nodosus
    Wilson Wong
    Australian Research Council Centre of Excellence in Structural and Functional Microbial Genomics, Monash University, Clayton 3800, Australia
    Acta Crystallogr Sect F Struct Biol Cryst Commun 66:289-93. 2010
    ..7 A, beta = 113.6 degrees for BprV and a = 38.5, b = 90.5, c = 44.1 A, beta = 109.9 degrees for BprB. The crystals of all four proteases contained one molecule in the asymmetric unit, with a solvent content ranging from 36 to 40%...
  59. pmc Environmental response and autoregulation of Clostridium difficile TxeR, a sigma factor for toxin gene expression
    Nagraj Mani
    Department of Molecular Biology and Microbiology, Tufts University School of Medicine, 136 Harrison Avenue, Boston, MA 02111, USA
    J Bacteriol 184:5971-8. 2002
    ..The increased level of TxeR then permits high-level expression of the toxin genes. The study of txeR gene regulation in C. difficile was made possible by introduction of a mobilizable, replicative plasmid via conjugation with E. coli...
  60. pmc Construction of an alpha toxin gene knockout mutant of Clostridium perfringens type A by use of a mobile group II intron
    Yue Chen
    Department of Infectious Diseases and Microbiology, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, PA 15261, USA
    Appl Environ Microbiol 71:7542-7. 2005
    ..Advantages of this technology, such as site specificity, relatively high frequency of insertion, and introduction of no antibiotic resistance genes into the chromosome, could facilitate construction of other C. perfringens mutants...
  61. pmc Highly conserved alpha-toxin sequences of avian isolates of Clostridium perfringens
    Scott A Sheedy
    Commonwealth Scientific and Industrial Research Organisation, Livestock Industries, Australian Animal Health Laboratory, Geelong 3220, Australia
    J Clin Microbiol 42:1345-7. 2004
    ..Analysis of the alpha-toxin of 25 chicken-derived C. perfringens strains demonstrated high homology to mammal-derived strains rather than to the only avian-derived C. perfringens alpha-toxin sequence reported previously...
  62. ncbi request reprint Revised nomenclature of Clostridium difficile toxins and associated genes
    Maja Rupnik
    Department of Biology, University of Ljubljana, Vecna pot 111, 1000 Ljubljana, Slovenia
    J Med Microbiol 54:113-7. 2005
    ..difficile toxins and other related large clostridial toxins produced by Clostridium sordellii and Clostridium novyi, and corresponding toxin genes, as well as toxin production types of C. difficile strains...
  63. pmc Both epsilon-toxin and beta-toxin are important for the lethal properties of Clostridium perfringens type B isolates in the mouse intravenous injection model
    Mariano E Fernandez-Miyakawa
    California Animal Health and Food Safety Laboratory San Bernardino Branch, University of California Davis, 105 West Central Avenue, San Bernardino, CA 92408, USA
    Infect Immun 75:1443-52. 2007
    ..However, our results suggest that both CPB and ETX are likely the most important contributors to the pathogenesis of C. perfringens type B infections in domestic animals...
  64. ncbi request reprint Genome sequence and identification of candidate vaccine antigens from the animal pathogen Dichelobacter nodosus
    Garry S A Myers
    The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, Maryland 20850, USA
    Nat Biotechnol 25:569-75. 2007
    ..Screening of these proteins against ovine antisera identified eight immunogenic proteins that are candidate antigens for a cross-protective vaccine...
  65. pmc Alpha-toxin of Clostridium perfringens is not an essential virulence factor in necrotic enteritis in chickens
    Anthony L Keyburn
    CSIRO Livestock Industries, Private Bag 24, Geelong, Victoria 3220, Australia
    Infect Immun 74:6496-500. 2006
    ..These results demonstrated that alpha-toxin is not an essential virulence factor in the pathogenesis of necrotic enteritis in chickens...
  66. pmc Development and application of an oral challenge mouse model for studying Clostridium perfringens type D infection
    Mariano E Fernandez-Miyakawa
    California Animal Health and Food Safety Laboratory System, San Bernardino Branch, University of California Davis, 105 West Central Avenue, San Bernardino, CA 92408, USA
    Infect Immun 75:4282-8. 2007
    ..This study provides an economical new model for studying the pathogenesis of C. perfringens type D infections...
  67. pmc Skewed genomic variability in strains of the toxigenic bacterial pathogen, Clostridium perfringens
    Garry S A Myers
    The Institute for Genomic Research, Rockville, Maryland 20850, USA
    Genome Res 16:1031-40. 2006
    ....
  68. ncbi request reprint Beta toxin is essential for the intestinal virulence of Clostridium perfringens type C disease isolate CN3685 in a rabbit ileal loop model
    Sameera Sayeed
    Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
    Mol Microbiol 67:15-30. 2008
    ..These results indicate that CPB is both required and sufficient for CN3685-induced enteric pathology, supporting a key role for this toxin in type C intestinal pathogenesis...
  69. pmc Epsilon-toxin is required for most Clostridium perfringens type D vegetative culture supernatants to cause lethality in the mouse intravenous injection model
    Sameera Sayeed
    E1240 BSTWR, University of Pittsburgh School of Medicine, Pittsburgh, PA, 15261, USA
    Infect Immun 73:7413-21. 2005
    ..Collectively, these results indicate that, under the experimental conditions used in the present study, ETX is necessary for the lethal properties of most genotype D vegetative supernatants in the mouse i.v. injection model...
  70. pmc Dissecting the contributions of Clostridium perfringens type C toxins to lethality in the mouse intravenous injection model
    Derek J Fisher
    Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
    Infect Immun 74:5200-10. 2006
    ..Collectively, our results highlight the importance of beta toxin for type C-induced toxemia...
  71. pmc Influence of gastric acid on susceptibility to infection with ingested bacterial pathogens
    Sharon M Tennant
    Department of Microbiology and Immunology, The University of Melbourne, and Murdoch Children s Research Institute, Royal Children s Hospital, Victoria 3010, Australia
    Infect Immun 76:639-45. 2008
    ....
  72. pmc NetB, a new toxin that is associated with avian necrotic enteritis caused by Clostridium perfringens
    Anthony L Keyburn
    CSIRO Livestock Industries, Australian Animal Health Laboratory, Geelong, Victoria, Australia
    PLoS Pathog 4:e26. 2008
    ..perfringens strains capable of causing NE. Furthermore, the netB mutant is the first rationally attenuated strain obtained in an NE-causing isolate of C. perfringens; as such it has considerable vaccine potential...