Affiliation: Monash University
- Mouse Germ Cell Development in-vivo and in-vitroDeshira Saiti
Monash Immunology and Stem Cell Laboratories, Level 3, STRIP 1 Buildings 75, Monash University, Wellington Rd, Clayton, Australia, 3800
Biomark Insights 2:241-52. 2007..This finding leads to the prospect of using ESC derived germ cells as a treatment for sterility. This review outlines the evolvement of germ cells from ESCs in vitro in relation to in vivo events...
- Oocyte activation after intracytoplasmic injection with sperm frozen without cryoprotectants results in live offspring from inbred and hybrid mouse strainsOrly Lacham-Kaplan
Institute of Reproduction and Development, Monash University, Clayton, 3168 Melbourne, Australia
Biol Reprod 69:1683-9. 2003..This may become an effective strategy for sperm preservation and the restoration of most popular strains used for genetic modifications...
- In vivo and in vitro differentiation of male germ cells in the mouseOrly Lacham-Kaplan
Monash Immunology and Stem Cell Laboratories, Monash University, 27 31 Wright Street, Clayton, Victoria 3165, Australia
Reproduction 128:147-52. 2004..Although no viable embryos were obtained from these gametes, the prospects are that this goal is not too far from being accomplished...
- Testicular cell conditioned medium supports differentiation of embryonic stem cells into ovarian structures containing oocytesOrly Lacham-Kaplan
Monash Immunology and Stem Cell Laboratories, Monash University, Wellington Rd, Clayton, Australia 3165
Stem Cells 24:266-73. 2006..The production of oocytes using this method is repeatable and reliable and may be applicable to other mammalian species, including the human...
- Reduced developmental competence of immature, in-vitro matured and postovulatory aged mouse oocytes following IVF and ICSIOrly Lacham-Kaplan
Monash Immunology and Stem Cell Laboratories, Monash University, Clayton, Victoria, Australia
Reprod Biol Endocrinol 6:58. 2008....
- Comparison of mice born after intracytoplasmic sperm injection with in vitro fertilization and natural matingTrevor J Wilson
Centre for Functional Genomics and Human Disease, Monash Institute of Medical Research, Monash University, Clayton, Victoria, Australia
Mol Reprod Dev 74:512-9. 2007..These observations would support the use of ICSI for derivation of mutant mouse lines and may be of some importance for the use of this technique in human ART...
- The employment of strontium to activate mouse oocytes: effects on spermatid-injection outcomeJean Loren
Monash Immunology and Stem Cell Laboratories, Level 3, STRIP 1 Building 75, Monash University, Wellington Rd, Clayton, Australia, 3800
Reproduction 131:259-67. 2006..5 h activation in strontium. The implementation of round-spermatid injection in combination with this oocyte-activation protocol led to live-birth outcomes not significantly different to those outcomes obtained by mature spermatozoa...
- Density gradients for the isolation of germ cells from embryoid bodiesDeshira Saiti
Monash Immunology and Stem Cell Laboratories, Level 3, STRIP 1, Building 75, Monash University, Wellington Road, Clayton, Australia 3800
Reprod Biomed Online 16:730-40. 2008..7 +/- 2.7% and 89.5 +/- 0.9% respectively. Although the purity of the isolated germ cells was similar between the two gradients, more germ cells with higher viability were obtained with the Percoll gradient...
- Gene expression profiling of human oocytes following in vivo or in vitro maturationGayle M Jones
Monash Immunology and Stem Cell Laboratories, Monash University, Level 3, STRIP Building 75 Wellington Road, Clayton, VIC 3800, Australia
Hum Reprod 23:1138-44. 2008..This developmental incompetence has been explained as poor oocyte cytoplasmic maturation without any determination of the likely molecular basis of this observation...