Andrew G Elefanty

Summary

Affiliation: Monash University
Country: Australia

Publications

  1. pmc Brachyury and related Tbx proteins interact with the Mixl1 homeodomain protein and negatively regulate Mixl1 transcriptional activity
    Lloyd A Pereira
    Differentiation and Transcription Laboratory, Peter MacCallum Cancer Centre and the Pathology Department, The University of Melbourne, Melbourne, Victoria, Australia
    PLoS ONE 6:e28394. 2011
  2. doi request reprint On the streets of San Francisco: highlights from the ISSCR Annual Meeting 2010
    Andrew G Elefanty
    Monash Immunology and Stem Cell Laboratories, Monash University, Clayton, Victoria, 3800, Australia
    Cell Stem Cell 7:443-50. 2010
  3. doi request reprint Pdgfrα and Flk1 are direct target genes of Mixl1 in differentiating embryonic stem cells
    Lloyd A Pereira
    Differentiation and Transcription Laboratory, Peter MacCallum Cancer Centre, East Melbourne, Victoria, 3002, Australia
    Stem Cell Res 8:165-79. 2012
  4. doi request reprint Generation of human embryonic stem cell reporter knock-in lines by homologous recombination
    Richard P Davis
    Monash Immunology and Stem Cell Laboratories, Monash University, Clayton, Australia
    Curr Protoc Stem Cell Biol . 2009
  5. doi request reprint Temporal restriction of pancreatic branching competence during embryogenesis is mirrored in differentiating embryonic stem cells
    Sue Mei Lim
    Monash Immunology and Stem Cell Laboratories MISCL, Monash University, Clayton, Victoria, Australia
    Stem Cells Dev 21:1662-74. 2012
  6. doi request reprint Enforced expression of Mixl1 during mouse ES cell differentiation suppresses hematopoietic mesoderm and promotes endoderm formation
    Sue Mei Lim
    Monash Immunology and Stem Cell Laboratories, Monash University, Clayton, Victoria, Australia
    Stem Cells 27:363-74. 2009
  7. ncbi request reprint Targeting a GFP reporter gene to the MIXL1 locus of human embryonic stem cells identifies human primitive streak-like cells and enables isolation of primitive hematopoietic precursors
    Richard P Davis
    Monash Immunology and Stem Cell Laboratories, Monash University, Clayton, Victoria, Australia
    Blood 111:1876-84. 2008
  8. doi request reprint APELIN promotes hematopoiesis from human embryonic stem cells
    Qing C Yu
    Monash Immunology and Stem Cell Laboratories, Monash University, Clayton, Australia
    Blood 119:6243-54. 2012
  9. doi request reprint Fourier transform infrared microspectroscopy reveals that tissue culture conditions affect the macromolecular phenotype of human embryonic stem cells
    Julie Cao
    Monash Immunology and Stem Cell Laboratories, Monash University, Building 75, STRIP 1, West Ring Road, Clayton, Victoria 3800, Australia
    Analyst 138:4147-60. 2013
  10. ncbi request reprint Endocrine cells develop within pancreatic bud-like structures derived from mouse ES cells differentiated in response to BMP4 and retinoic acid
    Suzanne J Micallef
    Monash University, Clayton, Victoria, Australia
    Stem Cell Res 1:25-36. 2007

Collaborators

Detail Information

Publications37

  1. pmc Brachyury and related Tbx proteins interact with the Mixl1 homeodomain protein and negatively regulate Mixl1 transcriptional activity
    Lloyd A Pereira
    Differentiation and Transcription Laboratory, Peter MacCallum Cancer Centre and the Pathology Department, The University of Melbourne, Melbourne, Victoria, Australia
    PLoS ONE 6:e28394. 2011
    ..Our results indicate that the activity of Mixl1 can be modulated by protein-protein interactions and that T-box factors can function as negative regulators of Mixl1 activity...
  2. doi request reprint On the streets of San Francisco: highlights from the ISSCR Annual Meeting 2010
    Andrew G Elefanty
    Monash Immunology and Stem Cell Laboratories, Monash University, Clayton, Victoria, 3800, Australia
    Cell Stem Cell 7:443-50. 2010
    ..The 2010 Annual Meeting of the International Society for Stem Cell Research (ISSCR) was held in San Francisco in June with an exciting program covering a wealth of stem cell research from basic science to clinical research...
  3. doi request reprint Pdgfrα and Flk1 are direct target genes of Mixl1 in differentiating embryonic stem cells
    Lloyd A Pereira
    Differentiation and Transcription Laboratory, Peter MacCallum Cancer Centre, East Melbourne, Victoria, 3002, Australia
    Stem Cell Res 8:165-79. 2012
    ..These data support the hypothesis that Mixl1 directly regulates Pdgfrα and Flk1 gene expression and strengthens the position of Mixl1 as a key regulator of mesendoderm development during mammalian gastrulation...
  4. doi request reprint Generation of human embryonic stem cell reporter knock-in lines by homologous recombination
    Richard P Davis
    Monash Immunology and Stem Cell Laboratories, Monash University, Clayton, Australia
    Curr Protoc Stem Cell Biol . 2009
    ..Reporter knock-in hESC lines are valuable tools that allow easy and rapid identification and isolation of specific hESC derivatives...
  5. doi request reprint Temporal restriction of pancreatic branching competence during embryogenesis is mirrored in differentiating embryonic stem cells
    Sue Mei Lim
    Monash Immunology and Stem Cell Laboratories MISCL, Monash University, Clayton, Victoria, Australia
    Stem Cells Dev 21:1662-74. 2012
    ....
  6. doi request reprint Enforced expression of Mixl1 during mouse ES cell differentiation suppresses hematopoietic mesoderm and promotes endoderm formation
    Sue Mei Lim
    Monash Immunology and Stem Cell Laboratories, Monash University, Clayton, Victoria, Australia
    Stem Cells 27:363-74. 2009
    ..Taken together, these studies suggest that high levels of Mixl1 preferentially allocate cells to the endoderm during ESC differentiation...
  7. ncbi request reprint Targeting a GFP reporter gene to the MIXL1 locus of human embryonic stem cells identifies human primitive streak-like cells and enables isolation of primitive hematopoietic precursors
    Richard P Davis
    Monash Immunology and Stem Cell Laboratories, Monash University, Clayton, Victoria, Australia
    Blood 111:1876-84. 2008
    ..These experiments demonstrate the utility of MIXL1(GFP/w) HESCs for analyzing the previously inaccessible events surrounding the development of human primitive streak-like cells and their subsequent commitment to hematopoiesis...
  8. doi request reprint APELIN promotes hematopoiesis from human embryonic stem cells
    Qing C Yu
    Monash Immunology and Stem Cell Laboratories, Monash University, Clayton, Australia
    Blood 119:6243-54. 2012
    ..Furthermore, APLN peptide also synergized with VEGF to promote the growth of hESC-derived endothelial cells. These studies identified APLN as a novel growth factor for hESC-derived hematopoietic and endothelial cells...
  9. doi request reprint Fourier transform infrared microspectroscopy reveals that tissue culture conditions affect the macromolecular phenotype of human embryonic stem cells
    Julie Cao
    Monash Immunology and Stem Cell Laboratories, Monash University, Building 75, STRIP 1, West Ring Road, Clayton, Victoria 3800, Australia
    Analyst 138:4147-60. 2013
    ....
  10. ncbi request reprint Endocrine cells develop within pancreatic bud-like structures derived from mouse ES cells differentiated in response to BMP4 and retinoic acid
    Suzanne J Micallef
    Monash University, Clayton, Victoria, Australia
    Stem Cell Res 1:25-36. 2007
    ..The readily detectable co-expression of GFP and RFP in grafts derived from transplanted EBs demonstrated the utility of Pdx1(GFP/w)Ins1(RFP/w) ESCs for investigating pancreatic differentiation in vitro and in vivo...
  11. doi request reprint NKX2-5(eGFP/w) hESCs for isolation of human cardiac progenitors and cardiomyocytes
    David A Elliott
    Monash Immunology and Stem Cell Laboratories, Monash University, Clayton, Victoria, Australia
    Nat Methods 8:1037-40. 2011
    ..We used NKX2-5 eGFP(+) cells to identify VCAM1 and SIRPA as cell-surface markers expressed in cardiac lineages...
  12. doi request reprint Derivation of endothelial cells from human embryonic stem cells in fully defined medium enables identification of lysophosphatidic acid and platelet activating factor as regulators of eNOS localization
    Magdaline Costa
    Monash Immunology and Stem Cell Laboratories MISCL, Monash University, Clayton, Victoria, Australia
    Stem Cell Res 10:103-17. 2013
    ..We have demonstrated a hitherto unrecognized role for LPA and PAF in the regulation of eNOS subcellular localization...
  13. doi request reprint A targeted NKX2.1 human embryonic stem cell reporter line enables identification of human basal forebrain derivatives
    Adam L Goulburn
    Monash Immunology and Stem Cell Laboratories, Monash University, Clayton, Victoria, Australia
    Stem Cells 29:462-73. 2011
    ..These studies highlight the diversity of cell types that can be generated from human NKX2.1(+) progenitors and demonstrate the utility of NKX2.1(GFP/w) hESCs for investigating human forebrain development and neuronal differentiation...
  14. pmc The characterisation of pluripotent and multipotent stem cells using Fourier transform infrared microspectroscopy
    Julie Cao
    Department of Anatomy and Developmental Biology, Faculty of Medicine, Nursing and Health Sciences, Monash University, Wellington Road, Clayton, Victoria 3800, Australia
    Int J Mol Sci 14:17453-76. 2013
    ..The use of this technique therefore contributes information that is complementary to that acquired by conventional genetic and immunohistochemical methods. ..
  15. ncbi request reprint Forced aggregation of defined numbers of human embryonic stem cells into embryoid bodies fosters robust, reproducible hematopoietic differentiation
    Elizabeth S Ng
    Monash Immunology and Stem Cell Laboratories, Monash University, Bldg 75, STRIP 1, West Ring Rd, Clayton, Victoria, 3800 Australia
    Blood 106:1601-3. 2005
    ..This method of EB formation provides a generally applicable means for modulating and objectively monitoring the directed differentiation of hESCs...
  16. doi request reprint A protocol for removal of antibiotic resistance cassettes from human embryonic stem cells genetically modified by homologous recombination or transgenesis
    Richard P Davis
    Monash Immunology and Stem Cell Laboratories, STRIP 1, Building 75, Level 3, Monash University, Clayton, Victoria, Australia
    Nat Protoc 3:1550-8. 2008
    ..An integrated procedure for the clonal isolation of these genetically modified lines using single-cell deposition flow cytometry is also detailed. When performed sequentially, these protocols take approximately 1 month...
  17. doi request reprint Expression from a betageo gene trap in the Slain1 gene locus is predominantly associated with the developing nervous system
    Claire E Hirst
    Monash Immunology and Stem Cell Laboratories, Monash University, West Ring Road, Clayton, Victoria 3800, Australia
    Int J Dev Biol 54:1383-8. 2010
    ..These observations suggest that Slain1 may play a role in the development of the nervous system, as well as in the morphogenesis of several embryonic structures...
  18. ncbi request reprint Differentiation of human embryonic stem cells in serum-free medium reveals distinct roles for bone morphogenetic protein 4, vascular endothelial growth factor, stem cell factor, and fibroblast growth factor 2 in hematopoiesis
    Marjorie Pick
    Monash Immunology and Stem Cell Laboratories, Monash University, Clayton, Victoria 3800, Australia
    Stem Cells 25:2206-14. 2007
    ..These data demonstrate the utility of the serum-free spin EB system in dissecting the roles of specific growth factors required for the directed differentiation of HESCs toward the hematopoietic lineage...
  19. doi request reprint Directed differentiation of human embryonic stem cells as spin embryoid bodies and a description of the hematopoietic blast colony forming assay
    Elizabeth S Ng
    Monash Immunology and Stem Cell Laboratories, Monash University, Clayton, Victoria, Australia
    Curr Protoc Stem Cell Biol . 2008
    ..Up to 40 plates can be set up within a couple of hours by one experimenter, and aliquots of the differentiating EBs can be harvested at intervals and subjected to analyses using a variety of techniques...
  20. ncbi request reprint Mixl1 and oct4 proteins are transiently co-expressed in differentiating mouse and human embryonic stem cells
    Anna K Mossman
    Monash Immunology and Stem Cell Laboratories MISCL, Monash University, Clayton, Victoria 3800, Australia
    Stem Cells Dev 14:656-63. 2005
    ..Furthermore, these studies confirmed that anti-Mixl1 antibodies are a valuable reagent for monitoring ESC differentiation and will facilitate the efficient generation of clinically relevant cell types...
  21. ncbi request reprint Transcriptional profiling of mouse and human ES cells identifies SLAIN1, a novel stem cell gene
    Claire E Hirst
    Monash Immunology and Stem Cell Laboratories, Monash University, Clayton, VIC 3800, Australia
    Dev Biol 293:90-103. 2006
    ..SLAIN1 and its more widely expressed homologue SLAIN2 comprise a new family of structurally unique genes conserved throughout vertebrate evolution...
  22. pmc Differentiating embryonic stem cells pass through 'temporal windows' that mark responsiveness to exogenous and paracrine mesendoderm inducing signals
    Steven A Jackson
    Monash Immunology and Stem Cell Laboratories, Monash University, Clayton, Victoria, Australia
    PLoS ONE 5:e10706. 2010
    ..Mesendoderm induction during embryonic stem cell (ESC) differentiation in vitro is stimulated by the Transforming Growth Factor and Wingless (Wnt) families of growth factors...
  23. doi request reprint The Mix family of homeobox genes--key regulators of mesendoderm formation during vertebrate development
    Lloyd A Pereira
    Differentiation and Transcription Laboratory, Peter MacCallum Cancer Centre and the Pathology Department, The University of Melbourne, Parkville, Victoria, Australia
    Dev Biol 367:163-77. 2012
    ....
  24. pmc Generation of megakaryocytic progenitors from human embryonic stem cells in a feeder- and serum-free medium
    Marjorie Pick
    Monash Immunology and Stem Cell Laboratories, Monash University, Clayton, Victoria, Australia
    PLoS ONE 8:e55530. 2013
    ..As an important step towards this goal, we report the differentiation of human embryonic stem cells (hESCs) towards the megakaryocyte (Mk) lineage using a 'spin embryoid body' method in serum-free differentiation medium...
  25. doi request reprint ErythRED, a hESC line enabling identification of erythroid cells
    Tanya Hatzistavrou
    Monash Immunology and Stem Cell Laboratories, Monash University, Clayton, Victoria, Australia
    Nat Methods 6:659-62. 2009
    ..We describe a genetically modified hESC line, ErythRED, in which expression of RFP, controlled by regulatory sequences from the human beta-globin locus control region, is restricted to maturing erythroid cells...
  26. ncbi request reprint Pancreas differentiation of mouse ES cells
    Suzanne J Micallef
    Monash University, Victoria, Australia
    Curr Protoc Stem Cell Biol . 2007
    ..This unit outlines a method for the differentiation of ESCs toward pancreatic endoderm in serum-free medium from embryoid bodies (EBs) formed in suspension or spin EBs. In addition there is a protocol for maintaining ESC...
  27. doi request reprint Expansion of human embryonic stem cells in vitro
    Magdaline Costa
    Monash University, Clayton, Victoria
    Curr Protoc Stem Cell Biol . 2008
    ..Nevertheless, enzymatic passaging can be used for short periods (up to 25 passages) without the appearance of cells with abnormal karyotypes...
  28. ncbi request reprint The primitive streak gene Mixl1 is required for efficient haematopoiesis and BMP4-induced ventral mesoderm patterning in differentiating ES cells
    Elizabeth S Ng
    Monash Immunology and Stem Cell Laboratories, Monash University, Clayton, VIC 3800, Australia
    Development 132:873-84. 2005
    ..In conclusion, this study has revealed an important role for Mixl1 in haematopoietic development and demonstrates the utility of the Mixl1(GFP/w) ES cells for evaluating growth factors influencing mesendodermal differentiation...
  29. pmc Generation of human female reproductive tract epithelium from human embryonic stem cells
    Louie Ye
    The Ritchie Centre, Monash Institute of Medical Research and Department of Obstetrics and Gynecology, Monash University, Melbourne, Australia
    PLoS ONE 6:e21136. 2011
    ..These progenitor cells are thought to be derived from Müllerian duct (MD), the primordial female reproductive tract (FRT)...
  30. ncbi request reprint The hESC line Envy expresses high levels of GFP in all differentiated progeny
    Magdaline Costa
    Monash Immunology and Stem Cell Laboratories, STRIP 1, West Ring Road, Monash University Campus, Clayton, Victoria, 3800, Australia
    Nat Methods 2:259-60. 2005
    ..We have generated a hESC line designated 'Envy', in which robust levels of green fluorescent protein (GFP) are expressed in stem cells and all differentiated progeny...
  31. ncbi request reprint A method for genetic modification of human embryonic stem cells using electroporation
    Magdaline Costa
    Monash Immunology and Stem Cell Laboratories, STRIP 1, West Ring Road, Monash University Campus, Clayton, Victoria 3800, Australia
    Nat Protoc 2:792-6. 2007
    ..The protocol we describe has been tested on four different HESC lines, and takes approximately 4 weeks from electroporation to PCR screening of G418-resistant clones...
  32. doi request reprint A protocol describing the use of a recombinant protein-based, animal product-free medium (APEL) for human embryonic stem cell differentiation as spin embryoid bodies
    Elizabeth S Ng
    Monash Immunology and Stem Cell Laboratories, STRIP 1, Building 75, Level 3, Monash University, Clayton, Victoria 3800, Australia
    Nat Protoc 3:768-76. 2008
    ..The integration of a medium that includes only defined and recombinant components with a defined number of cells to initiate EB formation results in a generally applicable, robust platform for growth factor-directed HESC differentiation...
  33. doi request reprint Building better beta cells
    Edouard G Stanley
    Monash Immunology and Stem Cell Laboratories, Level 3, Building 75, STRIP 1, West Ring Road, Monash University, Clayton, Victoria 3800, Australia
    Cell Stem Cell 2:300-1. 2008
    ..Recently, in Nature Biotechnology, Kroon et al. (2008) reported encouraging progress toward this goal with the development of functional islet-like structures in mice transplanted with hESC-derived pancreatic endoderm...
  34. doi request reprint Fourier transform infrared microspectroscopy identifies early lineage commitment in differentiating human embryonic stem cells
    Philip Heraud
    Monash Immunology and Stem Cell Laboratories, Monash University, Clayton, Victoria 3800, Australia
    Stem Cell Res 4:140-7. 2010
    ..FTIR spectroscopy has the potential to provide low-cost, automatable measurements for the quality control of stem and differentiated cells to be used in industry and regenerative medicine...
  35. ncbi request reprint Retinoic acid induces Pdx1-positive endoderm in differentiating mouse embryonic stem cells
    Suzanne J Micallef
    Centre for Early Human Development, Monash Institute of Reproduction and Development, 27 31 Wright St, Clayton, Victoria 3168, Australia
    Diabetes 54:301-5. 2005
    ..5 mouse embryo. These results demonstrate the utility of Pdx1(GFP/w) ES cells as a tool for monitoring the effects of factors that influence pancreatic differentiation from ES cells...
  36. ncbi request reprint SIRPA, VCAM1 and CD34 identify discrete lineages during early human cardiovascular development
    Rhys J P Skelton
    Murdoch Childrens Research Institute, The Royal Children s Hospital, Parkville, Victoria, Australia
    Stem Cell Res 13:172-9. 2014
    ..These markers identify specific stages of cardiomyocyte and endothelial lineage commitment and, thus provide a scaffold for establishing a fate map of early human cardiogenesis. ..
  37. ncbi request reprint Efficient generation of adipocytes in a dish
    Andrew G Elefanty
    Monash Immunology and Stem Cell Laboratories, Level 3, Building 75, West Ring Road, Monash University, Clayton, Victoria 3800, Australia
    Nat Cell Biol 14:126-7. 2012
    ....