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Genomes and Genes | Ian MacreadieSummaryCountry: Australia Publications
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Publications
Validation of folate in a convenient yeast assay suited for identification of inhibitors of Alzheimer's amyloid-beta aggregationIan Macreadie
CSIRO Molecular and Health Technologies and P Health Flagship, Parkville, Vic, Australia
J Alzheimers Dis 15:391-6. 2008..This result supports a protective role for folate and suggests that yeast assays may provide significant new approaches for testing of AD chemopreventatives...- Copper transport and Alzheimer's diseaseIan G Macreadie
CSIRO Molecular and Health Technologies, 343 Royal Parade, Parkville, Vic, Australia
Eur Biophys J 37:295-300. 2008..The transport of copper into the brain is complex and involves numerous players, including amyloid precursor protein, A beta peptide and cholesterol... - Growth inhibition of Candida species and Aspergillus fumigatus by statinsIan G Macreadie
CSIRO Health and Molecular and Technologies and P Health Flagship, Parkville, Vic, Australia
FEMS Microbiol Lett 262:9-13. 2006..Our findings suggest that the statins could have utility as antifungal agents and that fungal colonization could be affected in those on statin therapy... - A rapid assay for dihydropteroate synthase activity suitable for identification of inhibitorsRoss T Fernley
CSIRO, Molecular and Health Technologies, Parkville, VIC 3052, Australia
Anal Biochem 360:227-34. 2007.... - Alzheimer's Abeta fused to green fluorescent protein induces growth stress and a heat shock responseJo Caine
CSIRO P Health Flagship and CSIRO Molecular and Health Technologies, Parkville, Vic, Australia
FEMS Yeast Res 7:1230-6. 2007..These studies have relevance in understanding the role of Abeta in the death of neuronal cells, and indicate that yeast may be a new tractable model system for the screening for inhibitors of the stress caused by Abeta... - Saccharomyces cerevisiae expression vectors with thrombin-cleavable N- and C-terminal 6x(His) tagsOnisha Patel
CSIRO Health Sciences and Nutrition, 343 Royal Parade, Parkville, Victoria, Australia
Biotechnol Lett 25:331-4. 2003..To the parental yeast-E. coli shuttle vectors that have convenient copper-inducible expression, two selectable markers and LEU2d vector amplification, this development adds substantial versatility to product recovery... - Promoter strength of folic acid synthesis genes affects sulfa drug resistance in Saccharomyces cerevisiaePeter Iliades
CSIRO Health Sciences and Nutrition, Parkville, Victoria, Australia 3052
Microb Drug Resist 9:249-55. 2003..However, high FOL1 expression levels resulted in increased susceptibility to sulfamethoxazole in pABA free media. These data suggest that up-regulation of FOL1 expression can lead to sulfa drug resistance in Saccharomyces cerevisiae... - Inhibition studies of sulfonamide-containing folate analogs in yeastOnisha Patel
CSIRO Health Sciences and Nutrition and Biomolecular Research Institute, Parkville, Victoria, Australia
Microb Drug Resist 9:139-46. 2003..The diamino derivatives, which are precursors of the sulfa-DHP, were found to be DHFR inhibitors. These results suggest that a new class of drugs, based on DHP analogs, could be investigated... - Purification, properties, and crystallization of Saccharomyces cerevisiae dihydropterin pyrophosphokinase-dihydropteroate synthaseJanette Berglez
RMIT University, Bundoora, Vic, Australia
Protein Expr Purif 41:355-62. 2005..3) and these diffracted to 2.3A. Structural information from these crystals could be used to design novel drugs to inhibit folate biosynthesis... - Analysis in Escherichia coli of Plasmodium falciparum dihydropteroate synthase (DHPS) alleles implicated in resistance to sulfadoxineJanette Berglez
Biomolecular Research Institute, CSIRO Health Sciences and Nutrition, 343 Royal Parade, Parkville, Vic. 3052, Australia
Int J Parasitol 34:95-100. 2004..All mutant alleles remained sensitive to sulfachloropyridazine and sulfacetamide. These results suggest new drugs that could be tried for effective malaria treatment... - Novel endoperoxides: synthesis and activity against Candida speciesPeter Macreadie
CSIRO, Molecular and Health Technologies, Parkville, 3052 Victoria, Australia
Bioorg Med Chem Lett 16:920-2. 2006..This study of susceptibility to endoperoxide compounds presented here may lead to the development of potent new antifungal agents... - Isolation of the Pneumocystis carinii dihydrofolate synthase gene and functional complementation in Saccharomyces cerevisiaePhilippe M Hauser
Institute of Microbiology, University Hospital of Lausanne, Lausanne, Switzerland
FEMS Microbiol Lett 256:244-50. 2006..carinii mono-functional enzyme DHFS, to investigate a possible role of DHFS in the resistance to antifolates of P. jirovecii, the species infecting specifically humans, and to develop a new class of antifolates... - Simvastatin reduces ergosterol levels, inhibits growth and causes loss of mtDNA in Candida glabrataChristiane Westermeyer
CSIRO Health and Molecular and Technologies and P Health Flagship, Parkville, Victoria, Australia
FEMS Yeast Res 7:436-41. 2007..In addition, if C. glabrata is a valid model for studying statin treatments, it would be very useful for the preliminary screening of agents to reduce statin side-effects... - The three-dimensional structure of the bifunctional 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase/dihydropteroate synthase of Saccharomyces cerevisiaeMichael C Lawrence
CSIRO Health Sciences and Nutrition, 343 Royal Parade, Parkville, Victoria 3052, Australia
J Mol Biol 348:655-70. 2005..cerevisiae and probably other fungal species... - Analysis of Pneumocystis jirovecii DHPS alleles implicated in sulfamethoxazole resistance using an Escherichia coli model systemPeter Iliades
CSIRO Health Sciences and Nutrition, Parkville, Victoria, 3052, Australia
Microb Drug Resist 11:1-8. 2005..Two sulfa drugs (sulfachloropyridazine and sulfathiazole) were identified that had higher inhibitory potential than sulfamethoxazole, which is currently the preferred treatment for PCP... 
Mutations in the Pneumocystis jirovecii DHPS gene confer cross-resistance to sulfa drugsPeter Iliades
CSIRO Health Sciences and Nutrition, 343 Royal Parade, Parkville, Victoria 3052, Australia
Antimicrob Agents Chemother 49:741-8. 2005..We propose that SCP, SMP, and SDZ should be considered for clinical evaluation against PCP or for future development of novel sulfa drug compounds...- Cloning of the Pneumocystis jirovecii trifunctional FAS gene and complementation of its DHPS activity in Escherichia coliPeter Iliades
CSIRO Health Sciences and Nutrition, Parkville, Vic, Australia
Fungal Genet Biol 41:1053-62. 2004..Functional complementation allowed evaluation of DHPS mutations implicated with sulfa drug resistance... - Over-production of dihydrofolate reductase leads to sulfa-dihydropteroate resistance in yeastOnisha Patel
CSIRO Health Sciences and Nutrition, Parkville, Vic, Australia
FEMS Microbiol Lett 236:301-5. 2004..Over-production led to resistance to sulfa-DHP suggesting that sulfa-DHP targets DHFR. Spontaneous mutants hyper-resistant to sulfa-DHP did not show any changes within DHFR... - Dihydropteroate synthase mutations in Pneumocystis jiroveci can affect sulfamethoxazole resistance in a Saccharomyces cerevisiae modelPeter Iliades
CSIRO Health Sciences and Nutrition, 343 Royal Parade, Parkville, Victoria 3052, Australia
Antimicrob Agents Chemother 48:2617-23. 2004..Upregulated PABA synthesis was thus implicated as a mechanism for sulfa drug resistance for mutants having two DHPS substitutions... - Sulfa drugs strike more than onceOnisha G Patel
Royal Melbourne Institute of Technology University, Bundoora West Campus, Bundoora, Victoria 3083, Australia
Trends Parasitol 20:1-3. 2004 - Cytotoxicity of dihydropteroate in Saccharomyces cerevisiaeAnn M Bayly
CSIRO Health Sciences and Nutrition, 343 Royal Parade, Parkville, Vic, 3052, Australia
FEMS Microbiol Lett 213:189-92. 2002..Dihydropteroic acid itself appeared to be toxic since both of these mutants were also inhibited. These results suggest novel ways in which antifolate therapy may be developed... - A new method to measure cellular toxicity of non-fibrillar and fibrillar Alzheimer's Abeta using yeastPrashant Bharadwaj
CSIRO Molecular and Health Technologies and P Health Flagship, Parkville, Vic, Australia
J Alzheimers Dis 13:147-50. 2008..We show that extracellular, non-fibrillar Abeta causes a dose dependent loss of cell viability while fibrillar Abeta has low toxicity... - Direct integrin alphavbeta6-ERK binding: implications for tumour growthNuzhat Ahmed
Gynaecological Cancer Research Centre, The Royal Women's Hospital, Melbourne and University of Melbourne, Melbourne, Australia
Oncogene 21:1370-80. 2002..The physical interaction between integrin alphavbeta6 and ERK2 defines a novel paradigm of integrin-mediated signalling and provides a therapeutic target for cancer treatment... - Novel endoperoxide antimalarials: synthesis, heme binding, and antimalarial activityDennis K Taylor
Department of Chemistry, Adelaide University, South Australia, 5005, Australia
J Med Chem 47:1833-9. 2004..However, the level of antimalarial potency is not well correlated with these activities, suggesting that some of the endoperoxides may exert their antimalarial activities by a novel mechanism of action... - Design of endoperoxides with anti-Candida activityThomas D Avery
Department of Chemistry, The University of Adelaide, 5005 SA, Australia
Bioorg Med Chem 15:36-42. 2007..This information provided guidance for the further development of endoperoxide and epoxy-endoperoxides as topical antifungal agents... - Folic acid antagonism of sulfa drug treatmentsAnn M Bayly
Trends Parasitol 18:49-50. 2002