Ian Macreadie

Summary

Country: Australia

Publications

  1. ncbi request reprint Purification, properties, and crystallization of Saccharomyces cerevisiae dihydropterin pyrophosphokinase-dihydropteroate synthase
    Janette Berglez
    RMIT University, Bundoora, Vic, Australia
    Protein Expr Purif 41:355-62. 2005
  2. ncbi request reprint Validation of folate in a convenient yeast assay suited for identification of inhibitors of Alzheimer's amyloid-beta aggregation
    Ian Macreadie
    CSIRO Molecular and Health Technologies and P Health Flagship, Parkville, Vic, Australia
    J Alzheimers Dis 15:391-6. 2008
  3. ncbi request reprint Copper transport and Alzheimer's disease
    Ian G Macreadie
    CSIRO Molecular and Health Technologies, 343 Royal Parade, Parkville, Vic, Australia
    Eur Biophys J 37:295-300. 2008
  4. ncbi request reprint Growth inhibition of Candida species and Aspergillus fumigatus by statins
    Ian G Macreadie
    CSIRO Health and Molecular and Technologies and P Health Flagship, Parkville, Vic, Australia
    FEMS Microbiol Lett 262:9-13. 2006
  5. ncbi request reprint A rapid assay for dihydropteroate synthase activity suitable for identification of inhibitors
    Ross T Fernley
    CSIRO, Molecular and Health Technologies, Parkville, VIC 3052, Australia
    Anal Biochem 360:227-34. 2007
  6. ncbi request reprint Alzheimer's Abeta fused to green fluorescent protein induces growth stress and a heat shock response
    Jo Caine
    CSIRO P Health Flagship and CSIRO Molecular and Health Technologies, Parkville, Vic, Australia
    FEMS Yeast Res 7:1230-6. 2007
  7. ncbi request reprint Saccharomyces cerevisiae expression vectors with thrombin-cleavable N- and C-terminal 6x(His) tags
    Onisha Patel
    CSIRO Health Sciences and Nutrition, 343 Royal Parade, Parkville, Victoria, Australia
    Biotechnol Lett 25:331-4. 2003
  8. ncbi request reprint Promoter strength of folic acid synthesis genes affects sulfa drug resistance in Saccharomyces cerevisiae
    Peter Iliades
    CSIRO Health Sciences and Nutrition, Parkville, Victoria, Australia 3052
    Microb Drug Resist 9:249-55. 2003
  9. ncbi request reprint Inhibition studies of sulfonamide-containing folate analogs in yeast
    Onisha Patel
    CSIRO Health Sciences and Nutrition and Biomolecular Research Institute, Parkville, Victoria, Australia
    Microb Drug Resist 9:139-46. 2003
  10. ncbi request reprint Analysis in Escherichia coli of Plasmodium falciparum dihydropteroate synthase (DHPS) alleles implicated in resistance to sulfadoxine
    Janette Berglez
    Biomolecular Research Institute, CSIRO Health Sciences and Nutrition, 343 Royal Parade, Parkville, VIC 3052, Australia
    Int J Parasitol 34:95-100. 2004

Collaborators

Detail Information

Publications26

  1. ncbi request reprint Purification, properties, and crystallization of Saccharomyces cerevisiae dihydropterin pyrophosphokinase-dihydropteroate synthase
    Janette Berglez
    RMIT University, Bundoora, Vic, Australia
    Protein Expr Purif 41:355-62. 2005
    ..3) and these diffracted to 2.3A. Structural information from these crystals could be used to design novel drugs to inhibit folate biosynthesis...
  2. ncbi request reprint Validation of folate in a convenient yeast assay suited for identification of inhibitors of Alzheimer's amyloid-beta aggregation
    Ian Macreadie
    CSIRO Molecular and Health Technologies and P Health Flagship, Parkville, Vic, Australia
    J Alzheimers Dis 15:391-6. 2008
    ..This result supports a protective role for folate and suggests that yeast assays may provide significant new approaches for testing of AD chemopreventatives...
  3. ncbi request reprint Copper transport and Alzheimer's disease
    Ian G Macreadie
    CSIRO Molecular and Health Technologies, 343 Royal Parade, Parkville, Vic, Australia
    Eur Biophys J 37:295-300. 2008
    ..The transport of copper into the brain is complex and involves numerous players, including amyloid precursor protein, A beta peptide and cholesterol...
  4. ncbi request reprint Growth inhibition of Candida species and Aspergillus fumigatus by statins
    Ian G Macreadie
    CSIRO Health and Molecular and Technologies and P Health Flagship, Parkville, Vic, Australia
    FEMS Microbiol Lett 262:9-13. 2006
    ..Our findings suggest that the statins could have utility as antifungal agents and that fungal colonization could be affected in those on statin therapy...
  5. ncbi request reprint A rapid assay for dihydropteroate synthase activity suitable for identification of inhibitors
    Ross T Fernley
    CSIRO, Molecular and Health Technologies, Parkville, VIC 3052, Australia
    Anal Biochem 360:227-34. 2007
    ....
  6. ncbi request reprint Alzheimer's Abeta fused to green fluorescent protein induces growth stress and a heat shock response
    Jo Caine
    CSIRO P Health Flagship and CSIRO Molecular and Health Technologies, Parkville, Vic, Australia
    FEMS Yeast Res 7:1230-6. 2007
    ..These studies have relevance in understanding the role of Abeta in the death of neuronal cells, and indicate that yeast may be a new tractable model system for the screening for inhibitors of the stress caused by Abeta...
  7. ncbi request reprint Saccharomyces cerevisiae expression vectors with thrombin-cleavable N- and C-terminal 6x(His) tags
    Onisha Patel
    CSIRO Health Sciences and Nutrition, 343 Royal Parade, Parkville, Victoria, Australia
    Biotechnol Lett 25:331-4. 2003
    ..To the parental yeast-E. coli shuttle vectors that have convenient copper-inducible expression, two selectable markers and LEU2d vector amplification, this development adds substantial versatility to product recovery...
  8. ncbi request reprint Promoter strength of folic acid synthesis genes affects sulfa drug resistance in Saccharomyces cerevisiae
    Peter Iliades
    CSIRO Health Sciences and Nutrition, Parkville, Victoria, Australia 3052
    Microb Drug Resist 9:249-55. 2003
    ..However, high FOL1 expression levels resulted in increased susceptibility to sulfamethoxazole in pABA free media. These data suggest that up-regulation of FOL1 expression can lead to sulfa drug resistance in Saccharomyces cerevisiae...
  9. ncbi request reprint Inhibition studies of sulfonamide-containing folate analogs in yeast
    Onisha Patel
    CSIRO Health Sciences and Nutrition and Biomolecular Research Institute, Parkville, Victoria, Australia
    Microb Drug Resist 9:139-46. 2003
    ..The diamino derivatives, which are precursors of the sulfa-DHP, were found to be DHFR inhibitors. These results suggest that a new class of drugs, based on DHP analogs, could be investigated...
  10. ncbi request reprint Analysis in Escherichia coli of Plasmodium falciparum dihydropteroate synthase (DHPS) alleles implicated in resistance to sulfadoxine
    Janette Berglez
    Biomolecular Research Institute, CSIRO Health Sciences and Nutrition, 343 Royal Parade, Parkville, VIC 3052, Australia
    Int J Parasitol 34:95-100. 2004
    ..All mutant alleles remained sensitive to sulfachloropyridazine and sulfacetamide. These results suggest new drugs that could be tried for effective malaria treatment...
  11. ncbi request reprint Novel endoperoxides: synthesis and activity against Candida species
    Peter Macreadie
    CSIRO, Molecular and Health Technologies, Parkville, 3052 Victoria, Australia
    Bioorg Med Chem Lett 16:920-2. 2006
    ..This study of susceptibility to endoperoxide compounds presented here may lead to the development of potent new antifungal agents...
  12. ncbi request reprint Isolation of the Pneumocystis carinii dihydrofolate synthase gene and functional complementation in Saccharomyces cerevisiae
    Philippe M Hauser
    Institute of Microbiology, University Hospital of Lausanne, Lausanne, Switzerland
    FEMS Microbiol Lett 256:244-50. 2006
    ..carinii mono-functional enzyme DHFS, to investigate a possible role of DHFS in the resistance to antifolates of P. jirovecii, the species infecting specifically humans, and to develop a new class of antifolates...
  13. ncbi request reprint Simvastatin reduces ergosterol levels, inhibits growth and causes loss of mtDNA in Candida glabrata
    Christiane Westermeyer
    CSIRO Health and Molecular and Technologies and P Health Flagship, Parkville, Victoria, Australia
    FEMS Yeast Res 7:436-41. 2007
    ..In addition, if C. glabrata is a valid model for studying statin treatments, it would be very useful for the preliminary screening of agents to reduce statin side-effects...
  14. ncbi request reprint The three-dimensional structure of the bifunctional 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase/dihydropteroate synthase of Saccharomyces cerevisiae
    Michael C Lawrence
    CSIRO Health Sciences and Nutrition, 343 Royal Parade, Parkville, Victoria 3052, Australia
    J Mol Biol 348:655-70. 2005
    ..cerevisiae and probably other fungal species...
  15. ncbi request reprint Analysis of Pneumocystis jirovecii DHPS alleles implicated in sulfamethoxazole resistance using an Escherichia coli model system
    Peter Iliades
    CSIRO Health Sciences and Nutrition, Parkville, Victoria, 3052, Australia
    Microb Drug Resist 11:1-8. 2005
    ..Two sulfa drugs (sulfachloropyridazine and sulfathiazole) were identified that had higher inhibitory potential than sulfamethoxazole, which is currently the preferred treatment for PCP...
  16. pmc Mutations in the Pneumocystis jirovecii DHPS gene confer cross-resistance to sulfa drugs
    Peter Iliades
    CSIRO Health Sciences and Nutrition, 343 Royal Parade, Parkville, Victoria 3052, Australia
    Antimicrob Agents Chemother 49:741-8. 2005
    ..We propose that SCP, SMP, and SDZ should be considered for clinical evaluation against PCP or for future development of novel sulfa drug compounds...
  17. ncbi request reprint Cloning of the Pneumocystis jirovecii trifunctional FAS gene and complementation of its DHPS activity in Escherichia coli
    Peter Iliades
    CSIRO Health Sciences and Nutrition, Parkville, Vic, Australia
    Fungal Genet Biol 41:1053-62. 2004
    ..Functional complementation allowed evaluation of DHPS mutations implicated with sulfa drug resistance...
  18. ncbi request reprint Over-production of dihydrofolate reductase leads to sulfa-dihydropteroate resistance in yeast
    Onisha Patel
    CSIRO Health Sciences and Nutrition, Parkville, Vic, Australia
    FEMS Microbiol Lett 236:301-5. 2004
    ..Over-production led to resistance to sulfa-DHP suggesting that sulfa-DHP targets DHFR. Spontaneous mutants hyper-resistant to sulfa-DHP did not show any changes within DHFR...
  19. pmc Dihydropteroate synthase mutations in Pneumocystis jiroveci can affect sulfamethoxazole resistance in a Saccharomyces cerevisiae model
    Peter Iliades
    CSIRO Health Sciences and Nutrition, 343 Royal Parade, Parkville, Victoria 3052, Australia
    Antimicrob Agents Chemother 48:2617-23. 2004
    ..Upregulated PABA synthesis was thus implicated as a mechanism for sulfa drug resistance for mutants having two DHPS substitutions...
  20. ncbi request reprint Sulfa drugs strike more than once
    Onisha G Patel
    Royal Melbourne Institute of Technology University, Bundoora West Campus, Bundoora, Victoria 3083, Australia
    Trends Parasitol 20:1-3. 2004
  21. ncbi request reprint Cytotoxicity of dihydropteroate in Saccharomyces cerevisiae
    Ann M Bayly
    CSIRO Health Sciences and Nutrition, 343 Royal Parade, Parkville, Vic, 3052, Australia
    FEMS Microbiol Lett 213:189-92. 2002
    ..Dihydropteroic acid itself appeared to be toxic since both of these mutants were also inhibited. These results suggest novel ways in which antifolate therapy may be developed...
  22. ncbi request reprint A new method to measure cellular toxicity of non-fibrillar and fibrillar Alzheimer's Abeta using yeast
    Prashant Bharadwaj
    CSIRO Molecular and Health Technologies and P Health Flagship, Parkville, Vic, Australia
    J Alzheimers Dis 13:147-50. 2008
    ..We show that extracellular, non-fibrillar Abeta causes a dose dependent loss of cell viability while fibrillar Abeta has low toxicity...
  23. ncbi request reprint Direct integrin alphavbeta6-ERK binding: implications for tumour growth
    Nuzhat Ahmed
    Gynaecological Cancer Research Centre, The Royal Women s Hospital, Melbourne and University of Melbourne, Melbourne, Australia
    Oncogene 21:1370-80. 2002
    ..The physical interaction between integrin alphavbeta6 and ERK2 defines a novel paradigm of integrin-mediated signalling and provides a therapeutic target for cancer treatment...
  24. ncbi request reprint Novel endoperoxide antimalarials: synthesis, heme binding, and antimalarial activity
    Dennis K Taylor
    Department of Chemistry, Adelaide University, South Australia, 5005, Australia
    J Med Chem 47:1833-9. 2004
    ..However, the level of antimalarial potency is not well correlated with these activities, suggesting that some of the endoperoxides may exert their antimalarial activities by a novel mechanism of action...
  25. ncbi request reprint Design of endoperoxides with anti-Candida activity
    Thomas D Avery
    Department of Chemistry, The University of Adelaide, 5005 SA, Australia
    Bioorg Med Chem 15:36-42. 2007
    ..This information provided guidance for the further development of endoperoxide and epoxy-endoperoxides as topical antifungal agents...
  26. ncbi request reprint Folic acid antagonism of sulfa drug treatments
    Ann M Bayly
    Trends Parasitol 18:49-50. 2002