Adam J Karpala
- IFN-gamma enhances immune responses to E. coli infection in the chickenVijaya Janardhana
CSIRO, Livestock Industries, AAHL, Victoria, Australia
J Interferon Cytokine Res 27:937-46. 2007..Cumulatively, these findings are indicative of in vivo enhancement of immune responses due to ChIFN-gamma. However, administration of ChIFN-gamma protein did not mitigate the development of air sac lesions following E. coli infection...
- Molecular cloning, expression, and characterization of chicken IFN -lambdaAdam J Karpala
CSIRO, Livestock Industries, Australian Animal Health Laboratory, Geelong, Victoria, Australia
J Interferon Cytokine Res 28:341-50. 2008..Our results demonstrate that in the chicken, as in human, the antiviral activity demonstrated by ChIFN-lambda supports its inclusion in therapeutic strategies directed against viral infections...
- Highly pathogenic (H5N1) avian influenza induces an inflammatory T helper type 1 cytokine response in the chickenAdam J Karpala
CSIRO, Livestock Industries, Australian Animal Health Laboratory, Geelong, Australia
J Interferon Cytokine Res 31:393-400. 2011....
- Visualising single molecules of HIV-1 and miRNA nucleic acidsKate L Jones
Centre for Virology, Burnet Institute, Melbourne, Australia
BMC Cell Biol 14:21. 2013..Here, we offer a generic and highly sensitive visualization approach (oligo fluorescent in situ hybridization, O-FISH) that can be used to detect such nucleic acids using a single-oligonucleotide probe of 19-26 nucleotides in length...
- Identifying innate immune pathways of the chicken may lead to new antiviral therapiesAdam J Karpala
CSIRO, Livestock Industries, Australian Animal Health Laboratory, Private Bag 24, Geelong, Victoria 3220, Australia
Vet Immunol Immunopathol 148:100-9. 2012..This review outlines important mammalian antiviral mechanisms that have been modulated to strengthen viral immunity and highlights the potential application of these strategies in the chicken, especially in regards, to AI...
- Ontogeny of the interferon system in chickensAdam J Karpala
CSIRO, Livestock Industries, AAHL, 5, Portarlington Road, Geelong, Victoria, 3220 Australia
J Reprod Immunol 94:169-74. 2012....
- Immune responses to dsRNA: implications for gene silencing technologiesAdam J Karpala
CSIRO Livestock Industries, Australian Animal Health Laboratory, Geelong, Victoria
Immunol Cell Biol 83:211-6. 2005..Thus, it is beneficial to understand all aspects of the immune response to dsRNA in order to manipulate these systems and minimize unwanted non-specific effects...
- Characterization of chicken Mda5 activity: regulation of IFN-β in the absence of RIG-I functionalityAdam J Karpala
Commonwealth Scientific and Industrial Research Organisation, Livestock Industries, Australian Animal Health Laboratory, Victoria 3220, Australia
J Immunol 186:5397-405. 2011..Collectively, although Mda5 is functionally active in the chicken, the absence of an apparent RIG-I-like function may contribute to the chicken's susceptibility to highly pathogenic influenza...
- Activation of the TLR3 pathway regulates IFNbeta production in chickensAdam J Karpala
CSIRO, Livestock Industries, AAHL, 5, Portarlington Road, Victoria 3220, Australia
Dev Comp Immunol 32:435-44. 2008..The interrelationship between TLR3 and interferon as well as the observed increase in TLR3 and IFNbeta expression during H5N1 avian influenza infection indicates the importance of these molecules in viral infections in chickens...
- Toll-like receptor 7 ligands inhibit influenza A infection in chickensCameron R Stewart
CSIRO Livestock Industries, Australian Animal Health Laboratory, Geelong, Victoria, Australia
J Interferon Cytokine Res 32:46-51. 2012..These results demonstrate that nucleic acid-sensing TLR ligands show considerable potential as antivirals in chickens and could be incorporated into antiviral strategies...
- Immunostimulatory motifs enhance antiviral siRNAs targeting highly pathogenic avian influenza H5N1Cameron R Stewart
Infection and Immunity, The Commonwealth Scientific and Industrial Research Organisation Australian Animal Health Laboratory, Geelong, Victoria, Australia
PLoS ONE 6:e21552. 2011..These data demonstrate the ability of an immunostimulatory motif to improve the performance of an RNAi-based antiviral, a finding that may influence the design of future RNAi-based anti-influenza therapeutics...
- H5N1 infection causes rapid mortality and high cytokine levels in chickens compared to ducksSimon Burggraaf
CSIRO Australian Animal Health Laboratory, Private Bag 24, Geelong, Victoria 3220, Australia School of Veterinary Science, The University of Melbourne, Parkville, Victoria, Australia
Virus Res 185:23-31. 2014..These observations support that hypercytokinemia may contribute to pathogenesis in chickens, whilst the lower cytokine response in ducks may be a factor in their apparent resistance to disease and decreased mortality. ..