N R Sims

Summary

Affiliation: Flinders University
Country: Australia

Publications

  1. ncbi request reprint Mitochondrial contributions to tissue damage in stroke
    Neil R Sims
    Department of Medical Biochemistry, Center for Neuroscience, School of Medicine, Flinders Medical Research Institute, Flinders University, G P O Box 2100, Adelaide, SA 5001, Australia
    Neurochem Int 40:511-26. 2002
  2. ncbi request reprint Energy metabolism, oxidative stress and neuronal degeneration in Alzheimer's disease
    N R Sims
    Department of Medical Biochemistry, School of Medicine, Flinders University, Adelaide, Australia
    Neurodegeneration 5:435-40. 1996
  3. doi request reprint Mitochondria, oxidative metabolism and cell death in stroke
    Neil R Sims
    Centre for Neuroscience and Discipline of Medical Biochemistry, Flinders Medical Science and Technology, School of Medicine, Flinders University, Adelaide, South Australia, Australia
    Biochim Biophys Acta 1802:80-91. 2010
  4. doi request reprint Isolation of mitochondria from rat brain using Percoll density gradient centrifugation
    Neil R Sims
    Centre for Neuroscience and Department of Medical Biochemistry, School of Medicine, Flinders University, GPO Box 2100, Adelaide, South Australia 5001, Australia
    Nat Protoc 3:1228-39. 2008
  5. ncbi request reprint Mitochondrial glutathione: a modulator of brain cell death
    N R Sims
    Department of Medical Biochemistry and Centre for Neuroscience, Flinders University, Adelaide, Australia
    J Bioenerg Biomembr 36:329-33. 2004
  6. ncbi request reprint Impairment of brain mitochondrial function by hydrogen peroxide
    N R Sims
    Department of Medical Biochemistry and Centre for Neuroscience, School of Medicine, Flinders University, G P O Box 2100, South Australia, 5001, Adelaide, Australia
    Brain Res Mol Brain Res 77:176-84. 2000
  7. ncbi request reprint The antioxidant defences of brain mitochondria during short-term forebrain ischemia and recirculation in the rat
    N R Sims
    Department of Medical Biochemistry and Centre for Neuroscience, School of Medicine, Flinders University, GPO Box 2100, Adelaide, South Australia, Australia
    Brain Res Mol Brain Res 60:141-9. 1998
  8. ncbi request reprint Brain mitochondrial responses to postischemic reperfusion: a role for calcium and hydrogen peroxide?
    N R Sims
    Department of Medical Biochemistry, School of Medicine, Flinders University, Adelaide, South Australia
    Dev Neurosci 22:366-75. 2000
  9. doi request reprint Astrocytic responses to DNA delivery using nucleofection
    H Muyderman
    Centre for Neuroscience, Flinders Medical Science and Technology, School of Medicine, Flinders University, Adelaide, SA, Australia
    Neurochem Res 35:1771-9. 2010
  10. ncbi request reprint The metabolism of C-glucose by neurons and astrocytes in brain subregions following focal cerebral ischemia in rats
    Anna E Thoren
    Centre for Neuroscience and Department of Medical Biochemistry, School of Medicine, Flinders University, Adelaide, Australia
    J Neurochem 97:968-78. 2006

Collaborators

Detail Information

Publications25

  1. ncbi request reprint Mitochondrial contributions to tissue damage in stroke
    Neil R Sims
    Department of Medical Biochemistry, Center for Neuroscience, School of Medicine, Flinders Medical Research Institute, Flinders University, G P O Box 2100, Adelaide, SA 5001, Australia
    Neurochem Int 40:511-26. 2002
    ..Further characterization of these mitochondrial responses should help to elucidate the mechanisms of cell death due to cerebral ischemia and possibly point to novel sites for therapeutic interventions in stroke...
  2. ncbi request reprint Energy metabolism, oxidative stress and neuronal degeneration in Alzheimer's disease
    N R Sims
    Department of Medical Biochemistry, School of Medicine, Flinders University, Adelaide, Australia
    Neurodegeneration 5:435-40. 1996
    ..Whether this is a contributor to neurodegeneration or a consequence of it remains unresolved...
  3. doi request reprint Mitochondria, oxidative metabolism and cell death in stroke
    Neil R Sims
    Centre for Neuroscience and Discipline of Medical Biochemistry, Flinders Medical Science and Technology, School of Medicine, Flinders University, Adelaide, South Australia, Australia
    Biochim Biophys Acta 1802:80-91. 2010
    ....
  4. doi request reprint Isolation of mitochondria from rat brain using Percoll density gradient centrifugation
    Neil R Sims
    Centre for Neuroscience and Department of Medical Biochemistry, School of Medicine, Flinders University, GPO Box 2100, Adelaide, South Australia 5001, Australia
    Nat Protoc 3:1228-39. 2008
    ..Each of the procedures produces mitochondria that are well coupled and exhibit high rates of respiratory activity. The procedures require an initial setup time of 45-75 min and between 1 and 3 h for the mitochondrial isolation...
  5. ncbi request reprint Mitochondrial glutathione: a modulator of brain cell death
    N R Sims
    Department of Medical Biochemistry and Centre for Neuroscience, Flinders University, Adelaide, Australia
    J Bioenerg Biomembr 36:329-33. 2004
    ..Thus, this antioxidant pool provides a potentially useful target for therapeutic intervention...
  6. ncbi request reprint Impairment of brain mitochondrial function by hydrogen peroxide
    N R Sims
    Department of Medical Biochemistry and Centre for Neuroscience, School of Medicine, Flinders University, G P O Box 2100, South Australia, 5001, Adelaide, Australia
    Brain Res Mol Brain Res 77:176-84. 2000
    ..However, no significant change was seen in activity of either the pyruvate dehydrogenase complex or NADH-ubiquinone oxidoreductase (complex I) when measured directly following treatment of the mitochondria with hydrogen peroxide...
  7. ncbi request reprint The antioxidant defences of brain mitochondria during short-term forebrain ischemia and recirculation in the rat
    N R Sims
    Department of Medical Biochemistry and Centre for Neuroscience, School of Medicine, Flinders University, GPO Box 2100, Adelaide, South Australia, Australia
    Brain Res Mol Brain Res 60:141-9. 1998
    ....
  8. ncbi request reprint Brain mitochondrial responses to postischemic reperfusion: a role for calcium and hydrogen peroxide?
    N R Sims
    Department of Medical Biochemistry, School of Medicine, Flinders University, Adelaide, South Australia
    Dev Neurosci 22:366-75. 2000
    ..The effects of Ca(2+), but not H(2)O(2), were inhibited by cyclosporin A, again implicating the permeability transition in some of the mitochondrial changes...
  9. doi request reprint Astrocytic responses to DNA delivery using nucleofection
    H Muyderman
    Centre for Neuroscience, Flinders Medical Science and Technology, School of Medicine, Flinders University, Adelaide, SA, Australia
    Neurochem Res 35:1771-9. 2010
    ..However, considerable care is needed in designing and interpreting such studies because of long-lasting changes induced in key properties of these cells by the nucleofection process...
  10. ncbi request reprint The metabolism of C-glucose by neurons and astrocytes in brain subregions following focal cerebral ischemia in rats
    Anna E Thoren
    Centre for Neuroscience and Department of Medical Biochemistry, School of Medicine, Flinders University, Adelaide, Australia
    J Neurochem 97:968-78. 2006
    ..These findings indicate the presence of complex changes in energy metabolism that are likely to greatly influence the responses of neurons and astrocytes to temporary focal ischemia...
  11. doi request reprint Alterations in membrane potential in mitochondria isolated from brain subregions during focal cerebral ischemia and early reperfusion: evaluation using flow cytometry
    Diane R Lee
    Discipline of Medical Biochemistry, School of Medicine, Flinders University, GPO Box 2100, Adelaide, SA 5001, Australia
    Neurochem Res 34:1857-66. 2009
    ..It also demonstrates the ability to gain a sensitive measure of these mitochondrial changes using flow cytometry...
  12. ncbi request reprint Highly selective and prolonged depletion of mitochondrial glutathione in astrocytes markedly increases sensitivity to peroxynitrite
    Hakan Muyderman
    Centre for Neuroscience, Flinders Institute for Health and Medical Research, and Department of Medical Biochemistry, School of Medicine, Flinders University, 5001 Adelaide, SA, Australia
    J Neurosci 24:8019-28. 2004
    ..They demonstrate the critical importance of this metabolite pool in protecting against peroxynitrite-induced damage in astrocytes and indicate a key contribution in determining the activities of respiratory chain components...
  13. ncbi request reprint Losses of NG2 and NeuN immunoreactivity but not astrocytic markers during early reperfusion following severe focal cerebral ischemia
    Diane R Lee
    Centre for Neuroscience and Department of Medical Biochemistry, Flinders University, GPO Box 2100, Adelaide, South Australia 5001, Australia
    Brain Res 989:221-30. 2003
    ..These findings provide the first evidence of marked sensitivity of NG2-immunoreactivity to severe ischemia and suggest a greater initial resistance of astrocytes compared with neurons and NG2-positive glia to ischemia-reperfusion damage...
  14. ncbi request reprint Fluorocitrate-mediated astroglial dysfunction causes seizures
    John O Willoughby
    Centre for Neuroscience and Department of Medicine, Flinders University and Medical Centre, Adelaide, South Australia, Australia
    J Neurosci Res 74:160-6. 2003
    ..These results indicate that focal cerebrocortical astroglial dysfunction leads to focal epileptiform discharges and sometimes to convulsive seizures and that the process possibly depends on effects mediated by gap junctions...
  15. ncbi request reprint Mitochondrial glutathione protects against cell death induced by oxidative and nitrative stress in astrocytes
    Hakan Muyderman
    Centre for Neuroscience and Department of Medical Biochemistry, School of Medicine, Flinders University, Adelaide, Australia
    J Neurochem 102:1369-82. 2007
    ....
  16. ncbi request reprint Glutathione monoethylester prevents mitochondrial glutathione depletion during focal cerebral ischemia
    Michelle F Anderson
    Centre for Neuroscience, Flinders Medical Research Institute and Department of Medical Biochemistry, School of Medicine, Flinders University, SA, Adelaide, Australia
    Neurochem Int 44:153-9. 2004
    ....
  17. doi request reprint Mitochondrial glutathione uptake: characterization in isolated brain mitochondria and astrocytes in culture
    Alison L Wadey
    Centre for Neuroscience and Department of Medical Biochemistry, School of Medicine, Flinders University, Adelaide, South Australia, Australia
    J Neurochem 109:101-8. 2009
    ....
  18. ncbi request reprint Inhibition of nitric oxide synthase with 7-nitroindazole does not modify early metabolic recovery following focal cerebral ischemia in rats
    Stephen C Helps
    Department of Medical Biochemistry, Centre for Neuroscience, School of Medicine, Flinders University, GPO Box 2100, Adelaide, SA 5001, Australia
    Neurochem Res 32:663-70. 2007
    ..These findings indicate that inhibition of oxidative metabolism is probably not the primary site of the deleterious effects of nitric oxide and derivatives during early post-ischemic reperfusion...
  19. ncbi request reprint The effects of focal ischemia and reperfusion on the glutathione content of mitochondria from rat brain subregions
    Michelle F Anderson
    Centre for Neuroscience, Flinders Medical Research Institute and Department of Medical Biochemistry, School of Medicine, Flinders University, Adelaide, South Australia
    J Neurochem 81:541-9. 2002
    ..These findings are consistent with a role for the glutathione depletion in determining the susceptibility of brain tissue to focal ischemia...
  20. ncbi request reprint Increased substance P immunoreactivity and edema formation following reversible ischemic stroke
    R J Turner
    Department of Pathology, University of Adelaide, Adelaide, SA, Australia
    Acta Neurochir Suppl 96:263-6. 2006
    ..As such, inhibition of neurogenic inflammation may represent a novel therapeutic target for the treatment of edema following reversible, ischemic stroke...
  21. doi request reprint Connexin 43 regulates astrocytic migration and proliferation in response to injury
    B Homkajorn
    Centre for Neuroscience and Discipline of Medical Biochemistry, Flinders Medical Science and Technology, School of Medicine, Flinders University, Adelaide, South Australia, Australia
    Neurosci Lett 486:197-201. 2010
    ..The findings directly indicate a role for Connexin 43 in the astrocytic response to injury and suggest that modification of Connexin 43 expression might provide a therapeutic target to alter potentially deleterious astrocytic responses...
  22. ncbi request reprint Astrocytic function assessed from 1-14C-acetate metabolism after temporary focal cerebral ischemia in rats
    Anna E Thoren
    Centre for Neuroscience and Department of Medical Biochemistry, School of Medicine, Flinders University, Adelaide, Australia
    J Cereb Blood Flow Metab 25:440-50. 2005
    ..These results indicate that many astrocytes, particularly in the cortex, remain viable and capable of at least some complex oxidative metabolism during the first few hours of recirculation...
  23. ncbi request reprint Increased mitochondrial permeability in response to intrastriatal N-methyl-D-aspartate: detection based on accumulation of radiolabel from [3H]deoxyglucose
    Emad Zaidan
    Department of Medical Biochemistry, Centre for Neuroscience, School of Medicine, Flinders University, Adelaide, South Australia, Australia
    Neurochem Res 29:609-16. 2004
    ..At earlier times, the restricted permeability of the inner mitochondrial membrane was apparently preserved, at least sufficiently to prevent significant diffusion of metabolites between the cytoplasm and the matrix...
  24. ncbi request reprint Glutathione monoethyl ester provides neuroprotection in a rat model of stroke
    Michelle F Anderson
    Institute of Clinical Neuroscience, Goteborg University, Goteborg, Sweden
    Neurosci Lett 354:163-5. 2004
    ..Infarct size was reduced from 46% of the total ischaemic hemisphere in saline-treated animals to 16% following ester treatment. Thus, glutathione monoethyl ester provides neuroprotection following transient focal cerebral ischaemia...
  25. ncbi request reprint (-)-Epigallocatechin gallate as an intervention for the acute treatment of cerebral ischemia
    Rosanna M A Rahman
    The Department of Pharmacology and Toxicology, University of Otago, Dunedin, P O Box 913, New Zealand
    Neurosci Lett 382:227-30. 2005
    ..We therefore conclude that 50 mg kg(-1) EGCG is not a viable intervention for the acute treatment of cerebral ischemia, as it is likely to increase the risk of intracerebral hemorrhaging...