Michael F Fenech

Summary

Country: Australia

Publications

  1. ncbi request reprint Commentary on the SFTG international collaborative study on the in vitro micronucleus test: to Cyt-B or not to Cyt-B?
    Michael Fenech
    Genome Health and Nutrigenomics Project, CSIRO Human Nutrition, Adelaide BC, SA, Australia
    Mutat Res 607:9-12. 2006
  2. ncbi request reprint Cytokinesis-block micronucleus assay evolves into a "cytome" assay of chromosomal instability, mitotic dysfunction and cell death
    Michael Fenech
    CSIRO Human Nutrition, Genome Health Nutrigenomics Project, P O Box 10041, Adelaide BC, Adelaide, SA 5000, Australia
    Mutat Res 600:58-66. 2006
  3. ncbi request reprint The Genome Health Clinic and Genome Health Nutrigenomics concepts: diagnosis and nutritional treatment of genome and epigenome damage on an individual basis
    Michael Fenech
    Genome Health Nutrigenomics Laboratory, CSIRO Health Sciences and Nutrition, Cooperative Research Centre for Diagnostics, PO Box 10041, Adelaide BC, SA 5000, Australia
    Mutagenesis 20:255-69. 2005
  4. ncbi request reprint Cytokinesis-block micronucleus cytome assay
    Michael Fenech
    Genome Health Nutrigenomics Laboratory, CSIRO Human Nutrition, Food Science Australia, PO Box 10041, Adelaide 5000, South Australia, Australia
    Nat Protoc 2:1084-104. 2007
  5. ncbi request reprint Nutrition and genome health
    Michael Fenech
    CSIRO Human Nutrition, Adelaide, Australia
    Forum Nutr 60:49-65. 2007
  6. doi request reprint Nutriomes and personalised nutrition for DNA damage prevention, telomere integrity maintenance and cancer growth control
    Michael F Fenech
    CSIRO Food and Nutritional Sciences, 10041, Adelaide BC, SA, 5000, Australia
    Cancer Treat Res 159:427-41. 2014
  7. doi request reprint HUMN project initiative and review of validation, quality control and prospects for further development of automated micronucleus assays using image cytometry systems
    Michael Fenech
    CSIRO Preventative Health Flagship, PO Box 10041 Adelaide BC, 5000, Australia
    Int J Hyg Environ Health 216:541-52. 2013
  8. doi request reprint Folate (vitamin B9) and vitamin B12 and their function in the maintenance of nuclear and mitochondrial genome integrity
    Michael Fenech
    CSIRO Food and Nutritional Sciences, PO Box 10041 Adelaide BC, SA 5000, Australia
    Mutat Res 733:21-33. 2012
  9. pmc The potential role of the antioxidant and detoxification properties of glutathione in autism spectrum disorders: a systematic review and meta-analysis
    Penelope Ae Main
    Sansom Institute for Health Research, University of South Australia, City East Campus, Adelaide, SA 5000, Australia
    Nutr Metab (Lond) 9:35. 2012
  10. pmc A method for non-invasive genotyping of APCmin/+ mice using fecal samples
    Erin L Symonds
    Nutrigenomics and Nutrigenetics, CSIRO Food and Nutritional Sciences, Adelaide, South Australia
    Biol Proced Online 14:1. 2012

Detail Information

Publications85

  1. ncbi request reprint Commentary on the SFTG international collaborative study on the in vitro micronucleus test: to Cyt-B or not to Cyt-B?
    Michael Fenech
    Genome Health and Nutrigenomics Project, CSIRO Human Nutrition, Adelaide BC, SA, Australia
    Mutat Res 607:9-12. 2006
  2. ncbi request reprint Cytokinesis-block micronucleus assay evolves into a "cytome" assay of chromosomal instability, mitotic dysfunction and cell death
    Michael Fenech
    CSIRO Human Nutrition, Genome Health Nutrigenomics Project, P O Box 10041, Adelaide BC, Adelaide, SA 5000, Australia
    Mutat Res 600:58-66. 2006
    ....
  3. ncbi request reprint The Genome Health Clinic and Genome Health Nutrigenomics concepts: diagnosis and nutritional treatment of genome and epigenome damage on an individual basis
    Michael Fenech
    Genome Health Nutrigenomics Laboratory, CSIRO Health Sciences and Nutrition, Cooperative Research Centre for Diagnostics, PO Box 10041, Adelaide BC, SA 5000, Australia
    Mutagenesis 20:255-69. 2005
    ..The review concludes with a vision for a paradigm shift in disease prevention strategy based on the diagnosis and nutritional treatment of genome/epigenome damage on an individual basis, i.e. The Genome Health Clinic...
  4. ncbi request reprint Cytokinesis-block micronucleus cytome assay
    Michael Fenech
    Genome Health Nutrigenomics Laboratory, CSIRO Human Nutrition, Food Science Australia, PO Box 10041, Adelaide 5000, South Australia, Australia
    Nat Protoc 2:1084-104. 2007
    ..The procedure can take up to 5 days to complete...
  5. ncbi request reprint Nutrition and genome health
    Michael Fenech
    CSIRO Human Nutrition, Adelaide, Australia
    Forum Nutr 60:49-65. 2007
    ..e. Genome Health Clinics)...
  6. doi request reprint Nutriomes and personalised nutrition for DNA damage prevention, telomere integrity maintenance and cancer growth control
    Michael F Fenech
    CSIRO Food and Nutritional Sciences, 10041, Adelaide BC, SA, 5000, Australia
    Cancer Treat Res 159:427-41. 2014
    ....
  7. doi request reprint HUMN project initiative and review of validation, quality control and prospects for further development of automated micronucleus assays using image cytometry systems
    Michael Fenech
    CSIRO Preventative Health Flagship, PO Box 10041 Adelaide BC, 5000, Australia
    Int J Hyg Environ Health 216:541-52. 2013
    ..This review identifies recent progress, important limitations and steps that need to be taken into account to enable the successful universal implementation of automated micronucleus assays by image cytometry. ..
  8. doi request reprint Folate (vitamin B9) and vitamin B12 and their function in the maintenance of nuclear and mitochondrial genome integrity
    Michael Fenech
    CSIRO Food and Nutritional Sciences, PO Box 10041 Adelaide BC, SA 5000, Australia
    Mutat Res 733:21-33. 2012
    ..e. >400μg/day) and vitamin B12 (i.e. >2μg/day) depending on an individual's capacity to absorb and metabolise these vitamins which may vary due to genetic and epigenetic differences...
  9. pmc The potential role of the antioxidant and detoxification properties of glutathione in autism spectrum disorders: a systematic review and meta-analysis
    Penelope Ae Main
    Sansom Institute for Health Research, University of South Australia, City East Campus, Adelaide, SA 5000, Australia
    Nutr Metab (Lond) 9:35. 2012
    ..abstract:..
  10. pmc A method for non-invasive genotyping of APCmin/+ mice using fecal samples
    Erin L Symonds
    Nutrigenomics and Nutrigenetics, CSIRO Food and Nutritional Sciences, Adelaide, South Australia
    Biol Proced Online 14:1. 2012
    ..This non-invasive method of genotyping may be applied to other transgenic mouse models...
  11. pmc A quantitative PCR method for measuring absolute telomere length
    Nathan J O'Callaghan
    CSIRO Food and Nutritional Sciences, Nutritional Genomics Laboratory, PO Box 10041, Adelaide BC, SA, 5000, Australia
    Biol Proced Online 13:3. 2011
    ..Compared to the traditional relative TL qPCR assays, the aTL method described in this protocol enables a more direct comparison of results between experiments within and between laboratories...
  12. doi request reprint Folate, DNA damage and the aging brain
    Michael Fenech
    CSIRO Food and Nutritional Sciences, Nutritional Genomics, Gate 13, Kintore Avenue, PO Box 10041, Adelaide BC, SA 5000, Australia
    Mech Ageing Dev 131:236-41. 2010
    ..In this brief review the current evidence that folate deficiency and associated metabolites, such as homocysteine, may accelerate DNA damage and aging of the brain is explored and important knowledge gaps are identified...
  13. doi request reprint Dietary reference values of individual micronutrients and nutriomes for genome damage prevention: current status and a road map to the future
    Michael F Fenech
    Commonwealth Scientific and Industrial Research Organisation Food and Nutritional Sciences, Adelaide BC SA 5000, Australia
    Am J Clin Nutr 91:1438S-1454S. 2010
    ..The ultimate goal is to match the nutriome to the genome to optimize genome maintenance and to prevent pathologic amounts of DNA damage...
  14. doi request reprint The lymphocyte cytokinesis-block micronucleus cytome assay and its application in radiation biodosimetry
    Michael Fenech
    CSIRO, PO Box 10041, Adelaide BC SA 5000, Australia
    Health Phys 98:234-43. 2010
    ..A brief account is also given of current developments in the automation of this technique and important knowledge gaps that need attention to further enhance the applicability of this important method for radiation biodosimetry...
  15. doi request reprint A lifetime passion for micronucleus cytome assays--reflections from Down Under
    Michael Fenech
    CSIRO Human Nutrition, P O Box 10041, Adelaide BC, SA 5000, Australia
    Mutat Res 681:111-7. 2009
    ....
  16. ncbi request reprint Harmonisation of the micronucleus assay in human buccal cells--a Human Micronucleus (HUMN) project (www.humn.org) initiative commencing in 2007
    Michael Fenech
    CSIRO Human Nutrition, Adelaide, South Australia 5000, Australia
    Mutagenesis 22:3-4. 2007
  17. doi request reprint Report on the buccal micronucleus assay workshop organized by the International Human Micronucleus (HUMN) project--Antalya, Turkey 2007
    Michael Fenech
    Mutagenesis 24:199-201. 2009
    ..A follow-up workshop is planned at the 10th International Conference on Environmental Mutagens in Florence in 2009...
  18. ncbi request reprint Genome health nutrigenomics and nutrigenetics--diagnosis and nutritional treatment of genome damage on an individual basis
    Michael Fenech
    CSIRO, Human Nutrition, PO Box 10041, Adelaide BC, SA 5000, Australia
    Food Chem Toxicol 46:1365-70. 2008
    ..e. Genome Health Clinics...
  19. pmc Nutriomes and nutrient arrays - the key to personalised nutrition for DNA damage prevention and cancer growth control
    Michael F Fenech
    CSIRO Food and Nutritional Sciences, PO Box 10041 Adelaide BC, SA, 5000, Australia
    Genome Integr 1:11. 2010
    ....
  20. doi request reprint The HUMN and HUMNxL international collaboration projects on human micronucleus assays in lymphocytes and buccal cells--past, present and future
    Michael Fenech
    Department of Nutritional Genomics and DNA Damage Diagnostics, Commonwealth Scientific and Industrial Research Organisation Food and Nutritional Sciences, Adelaide BC, South Australia 5000, Australia
    Mutagenesis 26:239-45. 2011
    ..g. erythrocyte and nasal cells and (iv) prospective association studies with pregnancy complications, developmental defects, childhood cancers, cardiovascular disease and neurodegenerative diseases...
  21. doi request reprint Molecular mechanisms of micronucleus, nucleoplasmic bridge and nuclear bud formation in mammalian and human cells
    M Fenech
    Department of Nutritional Genomics and DNA Damage Diagnostics, Commonwealth Scientific and Industrial Research Organisation Food and Nutritional Sciences, Gate 13 Kintore Avenue, PO Box 10041, Adelaide BC, South Australia 5000, Australia
    Mutagenesis 26:125-32. 2011
    ..NBUD represent the process of elimination of amplified DNA, DNA repair complexes and possibly excess chromosomes from aneuploid cells...
  22. doi request reprint Micronuclei and their association with sperm abnormalities, infertility, pregnancy loss, pre-eclampsia and intra-uterine growth restriction in humans
    Michael Fenech
    CSIRO Food and Nutritional Sciences, Gate 13, Kintore Avenue, Adelaide SA 5000, Australia
    Mutagenesis 26:63-7. 2011
    ..In conclusion, more attention should be given to the possibility of using MN assays in peripheral blood lymphocytes and reproductive tissues as a biomarker of risk for infertility and pregnancy complications in humans...
  23. ncbi request reprint Low intake of calcium, folate, nicotinic acid, vitamin E, retinol, beta-carotene and high intake of pantothenic acid, biotin and riboflavin are significantly associated with increased genome instability--results from a dietary intake and micronucleus inde
    Michael Fenech
    CSIRO Health Sciences and Nutrition, Adelaide, Australia, Women s and Children s Hospital, Adelaide, Australia
    Carcinogenesis 26:991-9. 2005
    ..The results from this study illustrate the strong impact of a wide variety of micronutrients and their interactions on genome health, depending on the level of intake...
  24. doi request reprint The effect of age, gender, diet and lifestyle on DNA damage measured using micronucleus frequency in human peripheral blood lymphocytes
    Michael Fenech
    Department of Nutritional Genomics and DNA Damage Diagnostics, Commonwealth Scientific and Industrial Research Organisation Food and Nutritional Sciences, Gate 13 Kintore Avenue, PO Box 10041, Adelaide BC, South Australia 5000, Australia
    Mutagenesis 26:43-9. 2011
    ..Proper control for these factors is required to enable better measurement of the impact of other conditions, such as environmental exposure to genotoxins or a susceptible genetic background, on MN frequency in PBL...
  25. ncbi request reprint Aleurone flour increases red-cell folate and lowers plasma homocyst(e)ine substantially in man
    Michael Fenech
    CSIRO Health Sciences and Nutrition, PO Box 10041, Gouger Street, Adelaide BC, SA 5000, Australia
    Br J Nutr 93:353-60. 2005
    ..1 (8.2, 10.0) micromol/l at baseline to 6.8 (6.2, 7.5) micromol/l after 16 weeks. In conclusion, moderate dietary intake of ALF can increase red-cell folate and decrease plasma homocyst(e)ine substantially...
  26. doi request reprint Effect of common polymorphisms in folate uptake and metabolism genes on frequency of micronucleated lymphocytes in a South Australian cohort
    Varinderpal Dhillon
    Genome Health and Nutrigenomics Laboratory, CSIRO Human Nutrition, A Centre of Food Science Australia, Adelaide BC, SA, Australia
    Mutat Res 665:1-6. 2009
    ..5 per thousand) (P trend=0.015). The RFC G80A and MTR A2756G polymorphisms and their combinations may be important variables that substantially affect lymphocyte BN-MN frequency in this South Australian cohort...
  27. ncbi request reprint Lymphocytes of BRCA1 and BRCA2 germ-line mutation carriers, with or without breast cancer, are not abnormally sensitive to the chromosome damaging effect of moderate folate deficiency
    Sasja Beetstra
    CSIRO Human Nutrition, PO Box 10041, Gouger Street, Adelaide BC 5000, SA, Australia
    Carcinogenesis 27:517-24. 2006
    ..The results of this study suggest that moderate folate deficiency has a stronger effect on chromosomal instability than BRCA1 or BRCA2 mutations found in breast cancer families...
  28. ncbi request reprint Methylenetetrahydrofolate reductase C677T polymorphism, folic acid and riboflavin are important determinants of genome stability in cultured human lymphocytes
    Michiyo Kimura
    The National Institute of Health and Nutrition, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8636, Japan
    J Nutr 134:48-56. 2004
    ..042). This preliminary investigation suggests that MTHFR C677T polymorphism and riboflavin affect genome instability; however, the effect is relatively small compared with that of folic acid...
  29. doi request reprint Folate deficiency is associated with the formation of complex nuclear anomalies in the cytokinesis-block micronucleus cytome assay
    Caroline F Bull
    CSIRO Food and Nutritional Sciences, Adelaide, South Australia
    Environ Mol Mutagen 53:311-23. 2012
    ....
  30. doi request reprint Telomere length in lymphocytes of older South Australian men may be inversely associated with plasma homocysteine
    Caroline F Bull
    CSIRO Human Nutrition, Adelaide, South Australia
    Rejuvenation Res 12:341-9. 2009
    ..57, p = 0.004), but this effect was not observed in the younger cohort or in the older female group. These results provide evidence that telomere length of lymphocytes in older men may be adversely affected by HCY in vivo...
  31. doi request reprint Application and adaptation of the in vitro micronucleus assay for the assessment of nutritional requirements of cells for DNA damage prevention
    Caroline F Bull
    CSIRO Food and Nutritional Sciences, Gate 13 Kintore Avenue, Adelaide, South Australia 5000, Australia
    Mutagenesis 26:193-7. 2011
    ..In addition, we outline current knowledge gaps and technical limitations and propose future adaptations to enhance the applicability of the CBMN-Cyt method for in vivo predictions...
  32. ncbi request reprint In vitro micronucleus technique to predict chemosensitivity
    Michael Fenech
    CSIRO Health Science and Nutrition, South Australia, Australia
    Methods Mol Med 111:3-32. 2005
    ..The basic principles and methods (including detailed scoring criteria for all the genotoxicity and cytotoxicity end points) of the CBMN assay are described and areas for future development identified...
  33. doi request reprint Genome-health nutrigenomics and nutrigenetics: nutritional requirements or 'nutriomes' for chromosomal stability and telomere maintenance at the individual level
    Caroline Bull
    CSIRO Human Nutrition, PO Box 10041, Adelaide BC, South Australia 5000, Australia
    Proc Nutr Soc 67:146-56. 2008
    ..It is evident that developing effective strategies for defining nutrient doses and combinations or 'nutriomes' for genome-health maintenance at the individual level is essential for further progress in this research field...
  34. ncbi request reprint A comparison of folic acid and 5-methyltetrahydrofolate for prevention of DNA damage and cell death in human lymphocytes in vitro
    Xu Wang
    School of Life Sciences, Yunnan Normal University, Kunming, Yunnan, 650092, People s Republic of China
    Mutagenesis 18:81-6. 2003
    ....
  35. doi request reprint The effect of selenium, as selenomethionine, on genome stability and cytotoxicity in human lymphocytes measured using the cytokinesis-block micronucleus cytome assay
    Jing Wu
    Nutrigenomics Laboratory, CSIRO Human Nutrition, SA, Australia
    Mutagenesis 24:225-32. 2009
    ..Therefore, a cautious approach to supplementation with Se-met is required to ensure that optimal genome health is achieved without cytotoxic effects...
  36. doi request reprint Genome instability is increased in lymphocytes of women with polycystic ovary syndrome and is correlated with insulin resistance
    Lisa J Moran
    Research Centre for Reproductive Health, Discipline of Obstetrics and Gynaecology, University of Adelaide, Adelaide, Australia
    Mutat Res 639:55-63. 2008
    ..The potential genetic contributors to PCOS are unclear. We tested the hypothesis that genomic instability (chromosome malsegregation and DNA damage) is increased in PCOS...
  37. doi request reprint Buccal micronucleus cytome assay
    Philip Thomas
    CSIRO Human Nutrition, Adelaide, South Australia
    Nat Protoc 4:825-37. 2009
    ..The protocol in its current form takes approximately 4 h to complete from the time of buccal cell collection to the generation of stained slides for microscopic analysis...
  38. ncbi request reprint Folic acid deficiency increases chromosomal instability, chromosome 21 aneuploidy and sensitivity to radiation-induced micronuclei
    Sasja Beetstra
    CSIRO Health Sciences and Nutrition, P O Box 10041, Gouger Street, Adelaide BC 5000, SA, Australia
    Mutat Res 578:317-26. 2005
    ..The results of this study show that folate status has an important impact on chromosomal stability and is an important modifying factor of cellular sensitivity to radiation-induced genome damage...
  39. doi request reprint Increased consumption of wheat biofortified with selenium does not modify biomarkers of cancer risk, oxidative stress, or immune function in healthy Australian males
    Jing Wu
    Nutritional Genomics and Genome Damage Diagnostics Laboratory, CSIRO Human Nutrition, Food Science Australia, Adelaide, South Australia, Australia
    Environ Mol Mutagen 50:489-501. 2009
    ....
  40. ncbi request reprint Nucleoplasmic bridges are a sensitive measure of chromosome rearrangement in the cytokinesis-block micronucleus assay
    Philip Thomas
    CSIRO Health Sciences and Nutrition, PO Box 10041 Adelaide BC, Adelaide, SA 5000, Australia
    Mutagenesis 18:187-94. 2003
    ..The CBMN assay allows NPB measurement to be achieved reliably because inhibition of cytokinesis prevents the loss of NPB that would otherwise occur if cells were allowed to divide...
  41. ncbi request reprint A comparison of folic acid deficiency-induced genomic instability in lymphocytes of breast cancer patients and normal non-cancer controls from a Chinese population in Yunnan
    Xu Wang
    State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, People s Republic of China
    Mutagenesis 21:41-7. 2006
    ....
  42. doi request reprint Folate deficiency induces dysfunctional long and short telomeres; both states are associated with hypomethylation and DNA damage in human WIL2-NS cells
    Caroline F Bull
    CSIRO Animal, Food and Health Sciences, P O Box 10041, Adelaide BC, South Australia 5000, Australia and Michael F Fenech
    Cancer Prev Res (Phila) 7:128-38. 2014
    ....
  43. doi request reprint The interactive effect of alcohol and folic acid on genome stability in human WIL2-NS cells measured using the cytokinesis-block micronucleus cytome assay
    Theodora Teo
    CSIRO Human Nutrition, P O Box 10041, Adelaide BC, South Australia 5000, Australia
    Mutat Res 657:32-8. 2008
    ....
  44. ncbi request reprint Origin of nuclear buds and micronuclei in normal and folate-deprived human lymphocytes
    Hanna K Lindberg
    New Technologies and Risks, Work Environment Development, Finnish Institute of Occupational Health, Topeliuksenkatu 41aA, FI 00250 Helsinki, Finland
    Mutat Res 617:33-45. 2007
    ....
  45. doi request reprint Weight loss in obese men is associated with increased telomere length and decreased abasic sites in rectal mucosa
    Nathan J O'Callaghan
    CSIRO Human Nutrition and Food Science Australia, Adelaide, South Australia, Australia
    Rejuvenation Res 12:169-76. 2009
    ..56, p = 0.01, respectively). These results suggest that weight loss by caloric-restricted diets may contribute to the prevention of telomere shortening and DNA base damage, which are important initiating events in carcinogenesis...
  46. doi request reprint Effects of surface chemistry on cytotoxicity, genotoxicity, and the generation of reactive oxygen species induced by ZnO nanoparticles
    Hong Yin
    CSIRO Materials Science and Engineering, CSIRO Future Manufacturing Flagship, Clayton VIC 3168, Australia
    Langmuir 26:15399-408. 2010
    ..The lowest toxicity was achieved with a surface coating of components from a cell culture medium...
  47. ncbi request reprint Micronuclei, nucleoplasmic bridges and nuclear buds induced in folic acid deficient human lymphocytes-evidence for breakage-fusion-bridge cycles in the cytokinesis-block micronucleus assay
    Michael Fenech
    CSIRO Health Sciences and Nutrition, Genomic Stability Project, P O Box 10041, Adelaide BC, Australia
    Mutat Res 504:131-6. 2002
    ..These results also suggest that the CBMN assay may be a useful model for the study of the BFB cycle which may be one of the key mechanisms for the hypermutability phenotype required for the rapid evolution of cancer cells...
  48. doi request reprint Buccal micronucleus cytome assay
    Philip Thomas
    CSIRO Human Nutrition, Adelaide, SA, Australia
    Methods Mol Biol 682:235-48. 2011
    ..This protocol describes the current established methods for buccal cell collection, slide preparation, cellular and nuclear staining, and scoring criteria...
  49. ncbi request reprint Genomic stability: a new paradigm for recommended dietary allowances (RDAs)
    Michael Fenech
    CSIRO Health Sciences and Nutrition, Adelaide BC, SA, Australia
    Forum Nutr 56:97-100. 2003
  50. ncbi request reprint Chromosomal biomarkers of genomic instability relevant to cancer
    Michael Fenech
    Cooperative Research Centre for Diagnostics, CSIRO Health Sciences and Nutrition, PO Box 10041, Adelaide, BC, SA 5000, Australia
    Drug Discov Today 7:1128-37. 2002
    ..In addition, the techniques for measuring the various chromosomal instability events are discussed with a focus on the cytokinesis-block micronucleus assay as an almost complete system for measuring these various genetic mishaps...
  51. ncbi request reprint Biomarkers of genetic damage for cancer epidemiology
    Michael Fenech
    CSIRO Health Sciences and Nutrition, P O Box 10041, Gouger Street, SA 5000 Adelaide, BC, Australia
    Toxicology 181:411-6. 2002
    ..Controlling DNA damage rate to its possible minimum is likely to have an important impact in preventing cancer and other DNA damage-related degenerative diseases including ageing...
  52. doi request reprint High protein-high red meat versus high carbohydrate weight loss diets do not differ in effect on genome stability and cell death in lymphocytes of overweight men
    Bianca Benassi-Evans
    Commonwealth Scientific and Industrial Research Organisation, Human Nutrition, South Australia, Australia
    Mutagenesis 24:271-7. 2009
    ..The results suggest that the effect of the HP weight loss diet on DNA damage measured using the CBMN-Cyt assay in PBLs was not different from that observed for the HC weight loss diet...
  53. ncbi request reprint Buccal micronucleus cytome biomarkers may be associated with Alzheimer's disease
    Philip Thomas
    CSIRO Division of Human Nutrition, Adelaide, SA 5000, Australia
    Mutagenesis 22:371-9. 2007
    ..These promising results need to be replicated in larger studies and in cohorts of other neurodegenerative disorders to determine specificity of changes to Alzheimer's patients...
  54. doi request reprint A quantitative real-time PCR method for absolute telomere length
    Nathan O'Callaghan
    Commonwealth Scientific and Industrial Research Organization CSIRO Human Nutrition, Adelaide, Australia
    Biotechniques 44:807-9. 2008
    ..The capability to generate absolute telomere length values should allow a more direct comparison of results between experiments within and between laboratories...
  55. ncbi request reprint The micronucleus assay determination of chromosomal level DNA damage
    Michael Fenech
    CSIRO Human Nutrition, Adelaide BC, South Australia, Australia
    Methods Mol Biol 410:185-216. 2008
    ..The basic principles and methods (including detailed scoring criteria for all the genotoxicity and cytotoxicity end points) of the CBMN assay are described and areas for future development identified...
  56. ncbi request reprint The buccal cytome and micronucleus frequency is substantially altered in Down's syndrome and normal ageing compared to young healthy controls
    Philip Thomas
    CSIRO Human Nutrition, PO Box 10041, Adelaide BC, Adelaide, SA 5000, Australia
    Mutat Res 638:37-47. 2008
    ..This study validates the use of a cytome approach to investigate DNA damage, cell death and cell proliferation in buccal cells with ageing...
  57. doi request reprint Telomere length in white blood cells, buccal cells and brain tissue and its variation with ageing and Alzheimer's disease
    Philip Thomas
    CSIRO Human Nutrition, Adelaide BC, Adelaide, SA, Australia
    Mech Ageing Dev 129:183-90. 2008
    ..These results need to be replicated in larger studies and in cohorts of other neurodegenerative disorders to determine specificity of changes to Alzheimer's patients...
  58. doi request reprint Methionine-dependence phenotype in the de novo pathway in BRCA1 and BRCA2 mutation carriers with and without breast cancer
    Sasja Beetstra
    Commonwealth Scientific and Industrial Research Organisation Human Nutrition, South Australia 5000, Australia
    Cancer Epidemiol Biomarkers Prev 17:2565-71. 2008
    ..76-13.9) and 3.9 (P=0.09; 95% confidence interval, 0.93-16.3), respectively]. The results of this study support the hypothesis that defects in methionine metabolism may be associated with breast cancer risk in BRCA carriers...
  59. ncbi request reprint Chromosome 17 and 21 aneuploidy in buccal cells is increased with ageing and in Alzheimer's disease
    Philip Thomas
    CSIRO Human Nutrition, PO Box 10041, Adelaide BC, Adelaide, South Australia 5000, Australia
    Mutagenesis 23:57-65. 2008
    ....
  60. ncbi request reprint A review of genome mutation and Alzheimer's disease
    Philip Thomas
    CSIRO Health Sciences and Nutrition, PO Box 10041 Adelaide BC, Adelaide, South Australia 5000, Australia
    Mutagenesis 22:15-33. 2007
    ..These biomarkers could then be used in diagnosis and the evaluation of potentially effective preventative measures...
  61. doi request reprint Effect of dietary intervention on human micronucleus frequency in lymphocytes and buccal cells
    Philip Thomas
    CSIRO Division of Food and Nutritional Sciences, PO Box 10041, 13 Kintore Avenue, Adelaide BC, Adelaide, South Australia 5000, Australia
    Mutagenesis 26:69-76. 2011
    ..Important knowledge gaps and future research directions are also explored. The outcomes of these studies suggest that supplementation with antioxidant vitamins and certain B vitamins may cause a substantial reduction in MN frequency...
  62. doi request reprint High-protein/high red meat and high-carbohydrate weight-loss diets do not differ in their effect on faecal water genotoxicity tested by use of the WIL2-NS cell line and with other biomarkers of bowel health
    Bianca Benassi-Evans
    CSIRO Food and Nutritional Sciences, PO Box 10041, Adelaide, South Australia 5000, Australia
    Mutat Res 703:130-6. 2010
    ....
  63. doi request reprint Genetic polymorphisms of genes involved in DNA repair and metabolism influence micronucleus frequencies in human peripheral blood lymphocytes
    Varinderpal S Dhillon
    CSIRO Food and Nutritional Sciences, Gate 13, Kintore Avenue, PO Box 10041, Adelaide, South Australia 5000, Australia
    Mutagenesis 26:33-42. 2011
    ..These results contribute substantial evidence to the hypothesis that genotype may influence MN frequency in human cells...
  64. ncbi request reprint Uracil in DNA, determined by an improved assay, is increased when deoxynucleosides are added to folate-deficient cultured human lymphocytes
    Susan T Mashiyama
    Department of Cell and Molecular Biology, University of California, Berkley, CA 94720, USA
    Anal Biochem 330:58-69. 2004
    ..The assay requires 3 microg of DNA per folate-deficient sample, but more may be required for baseline DNA-uracil detection in healthy humans...
  65. ncbi request reprint hOGG1(326), XRCC1(399) and XRCC3(241) polymorphisms influence micronucleus frequencies in human lymphocytes in vivo
    Raluca A Mateuca
    Laboratorium voor Cellulaire Genetica, Vrije Universiteit Brussel, Pleinlaan 2, B 1050 Brussel, Belgium
    Mutagenesis 23:35-41. 2008
    ..Understanding the complexity of the relationships between exposure, DNA repair and MN frequencies require larger scale studies and complementary biomarkers...
  66. ncbi request reprint Folate deficiency induces aneuploidy in human lymphocytes in vitro-evidence using cytokinesis-blocked cells and probes specific for chromosomes 17 and 21
    Xu Wang
    State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, PR China
    Mutat Res 551:167-80. 2004
    ..MF was not more efficient than FA in preventing aneuploidy in this in vitro system. We conclude that folate deficiency is a risk factor for chromosomes 17 and 21 aneuploidy...
  67. pmc Cytokinesis-blocked micronucleus cytome assay biomarkers identify lung cancer cases amongst smokers
    Randa A El-Zein
    Department of Epidemiology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030 1439, USA
    Cancer Epidemiol Biomarkers Prev 17:1111-9. 2008
    ..9-100.0), PPV = 98.7 and NPV = 95.6. The simplicity, rapidity, and sensitivity of the assay together with potential for automation make it a valuable tool for screening and prioritizing potential cases for intensive screening...
  68. ncbi request reprint An increased micronucleus frequency in peripheral blood lymphocytes predicts the risk of cancer in humans
    Stefano Bonassi
    Unit of Molecular Epidemiology, National Cancer Research Institute, Genoa, Italy
    Carcinogenesis 28:625-31. 2007
    ..The current wide-spread use of the MN assay provides a valuable opportunity to apply this assay in the planning and validation of cancer surveillance and prevention programs...
  69. pmc Necrosis is increased in lymphoblastoid cell lines from children with autism compared with their non-autistic siblings under conditions of oxidative and nitrosative stress
    Penelope A E Main
    Sansom Institute of Health Research, University of South Australia, North Terrace, Adelaide, Australia
    Mutagenesis 28:475-84. 2013
    ....
  70. doi request reprint A review of methionine dependency and the role of methionine restriction in cancer growth control and life-span extension
    Paul Cavuoto
    CSIRO Food and Nutritional Sciences, P O Box 10041, Adelaide BC, SA 5000, Australia
    Cancer Treat Rev 38:726-36. 2012
    ..The application of nutritional methionine restriction and methioninase in combination with chemotherapeutic regimens is the current focus of clinical studies...
  71. doi request reprint Grape seed polyphenols and curcumin reduce genomic instability events in a transgenic mouse model for Alzheimer's disease
    Philip Thomas
    CSIRO Human Nutrition, PO Box 10041, Adelaide BC, Adelaide, SA 5000, Australia
    Mutat Res 661:25-34. 2009
    ..These results suggest potential protective effects of polyphenols against genomic instability events in different somatic tissues of a transgenic mouse model for AD...
  72. ncbi request reprint Intra- and inter-laboratory variation in the scoring of micronuclei and nucleoplasmic bridges in binucleated human lymphocytes. Results of an international slide-scoring exercise by the HUMN project
    Michael Fenech
    CSIRO Health Sciences and Nutrition, Adelaide, SA, Australia
    Mutat Res 534:45-64. 2003
    ....
  73. ncbi request reprint Cytokinesis-block micronucleus cytome assays for the determination of genotoxicity and cytotoxicity of cecal water in rats and fecal water in humans
    Bianca Benassi
    Division of Human Nutrition, Commonwealth Scientific and Industrial Research Organisation, P O Box 10041, Gouger Street, Adelaide, South Australia 5000, Australia
    Cancer Epidemiol Biomarkers Prev 16:2676-80. 2007
    ....
  74. doi request reprint Methylenetetrahydrofolate reductase, common polymorphisms, and relation to disease
    Philip Thomas
    CSIRO Human Nutrition, Adelaide BC, Adelaide, South Australia 5000
    Vitam Horm 79:375-92. 2008
    ..In this chapter, we consider the role that MTHFR plays in relation to folate metabolism and the possible contribution made in relation to certain important clinical outcomes...
  75. ncbi request reprint The case for strategic international alliances to harness nutritional genomics for public and personal health
    Jim Kaput
    Center of Excellence in Nutritional Genomics, University of California, Davis, CA 95616, USA
    Br J Nutr 94:623-32. 2005
    ....
  76. ncbi request reprint The effects of GSTM1 and GSTT1 polymorphisms on micronucleus frequencies in human lymphocytes in vivo
    Micheline Kirsch-Volders
    Laboratory of Cell Genetics, Vrije Universiteit Brussel, Pleinlaan 2, B 1050 Brussels, Belgium
    Cancer Epidemiol Biomarkers Prev 15:1038-42. 2006
    ..79). This study confirms that pooled analyses, by increasing the statistical power, are adequate for assessing the involvement of genetic variants on genome stability and for resolving discrepancies among individual studies...
  77. ncbi request reprint Report from the in vitro micronucleus assay working group
    Micheline Kirsch-Volders
    Laboratorium voor Cellulaire Genetica, Vrije Universiteit Brussel, Pleinlaan 2, Brussels 1050, Belgium
    Mutat Res 540:153-63. 2003
    ..7. Statistics: statistical significance should not be the sole factor for determining positive results. Biological meaning should serve as a guideline. Examples of statistical analyses are given...
  78. ncbi request reprint The principal phenolic and alcoholic components of wine protect human lymphocytes against hydrogen peroxide- and ionizing radiation-induced DNA damage in vitro
    William Greenrod
    CSIRO Health Sciences and Nutrition, PO Box 10041, Adelaide BC, South Australia 5000, Australia
    Mutagenesis 18:119-26. 2003
    ..e. hydrogen peroxide and ionizing radiation, in this physiologically relevant in vitro system...
  79. doi request reprint Consumption of grape seed extract prevents amyloid-beta deposition and attenuates inflammation in brain of an Alzheimer's disease mouse
    Yan Jiang Wang
    Department of Human Physiology and Centre for Neuroscience, Flinders University, GPO Box 2100, Adelaide, SA, Australia
    Neurotox Res 15:3-14. 2009
    ..Conclusively, polyphenol-rich GSE prevents the Abeta deposition and attenuates the inflammation in the brain of a transgenic mouse model, and this thus is promising in delaying development of AD...
  80. doi request reprint The micronucleus assay in human buccal cells as a tool for biomonitoring DNA damage: the HUMN project perspective on current status and knowledge gaps
    Nina Holland
    School of Public Health, University of California, Berkeley, CA 94720 7460, USA
    Mutat Res 659:93-108. 2008
    ....
  81. doi request reprint One-carbon metabolism enzyme polymorphisms and uteroplacental insufficiency
    Denise L F Furness
    Discipline of Obstetrics and Gynaecology, School of Paediatrics and Reproductive Health, The University of Adelaide, Adelaide, South Australia, Australia
    Am J Obstet Gynecol 199:276.e1-8. 2008
    ..This study was undertaken to test novel genetic polymorphisms involved in 1-carbon metabolism for a potential association with increased risk of developing pregnancy complications associated with uteroplacental insufficiency...
  82. ncbi request reprint Effect of smoking habit on the frequency of micronuclei in human lymphocytes: results from the Human MicroNucleus project
    Stefano Bonassi
    Department of Environmental Epidemiology, Istituto Nazionale per la Ricerca sul Cancro, Largo R Benzi, Genoa, Italy
    Mutat Res 543:155-66. 2003
    ..The presence of an interaction between smoking habit and occupational exposure to genotoxic agents should be always tested...
  83. doi request reprint Folate and methionine metabolism in autism: a systematic review
    Penelope A E Main
    Sansom Institute, University of South Australia, Adelaide, Australia
    Am J Clin Nutr 91:1598-620. 2010
    ..Recent evidence has emerged that children with autism may have altered folate or methionine metabolism, which suggests the folate-methionine cycle may play a key role in the etiology of autism...
  84. ncbi request reprint Health benefits of herbs and spices: the past, the present, the future
    Linda C Tapsell
    National Centre of Excellence in Functional Foods, University of Wollongong, NSW
    Med J Aust 185:S4-24. 2006
    ..Vegetable dishes and vegetarian options may be more appetising when prepared with herbs and spices...
  85. ncbi request reprint Anti-genotoxic effects of tea catechins against reactive oxygen species in human lymphoblastoid cells
    Ayako Sugisawa
    Department of Food Science Research for Health, IAI National Institute of Health and Nutrition, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8636, Japan
    Mutat Res 559:97-103. 2004
    ..These results suggest that physiological concentration of tea constituents are not genotoxic but rather anti-genotoxic against ROS, although their preventive effects are slightly different depending on their chemical structure...