Research Topics
Genomes and Genes | Stefan BroerSummaryAffiliation: Australian National University Country: Australia Publications
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Publications
Asymmetry of glutamine transporters in cultured neural cellsTobias Heckel
School of Biochemistry and Molecular Biology, Australian National University, Canberra, ACT 0200, Australia
Neurochem Int 43:289-98. 2003....- Persistence of the common Hartnup disease D173N allele in populations of European originDimitar N Azmanov
Medical Genetics Research Unit, ANU Medical School, Canberra, Australia
Ann Hum Genet 71:755-61. 2007..We postulate that survival of this allele may be a consequence of failure of the allele to completely inactivate the transport of neutral amino acids... - Transfer of glutamine between astrocytes and neuronsS Broer
Division of Biochemistry and Molecular Biology, Faculty of Science, Australian National University, Canberra, Australia
J Neurochem 77:705-19. 2001..It is likely that the ASCT2 transporter, an obligate exchanger of neutral amino acids, displaces the SN1 transporter as the main carrier of glutamine export in proliferating astrocytes... 
Apical transporters for neutral amino acids: physiology and pathophysiologyStefan Broer
School of Biochemistry and Molecular Biology, Australian National University, Canberra, Australia
Physiology (Bethesda) 23:95-103. 2008..Recent advances in the molecular identification of apical neutral amino acid transporters has shed a light on the molecular basis of Hartnup disorder and iminoglycinuria...- Amino acid transport across mammalian intestinal and renal epitheliaStefan Broer
School of Biochemistry and Molecular Biology, Australian National University, Canberra, Australian Capital Territory, Australia
Physiol Rev 88:249-86. 2008..With very few exceptions, individual amino acids are transported by more than one transporter, providing backup capacity for absorption in the case of mutational inactivation of a transport system... - The role of the neutral amino acid transporter B0AT1 (SLC6A19) in Hartnup disorder and protein nutritionStefan Broer
School of Biology, College of Medicine, Biology and Environment, Australian National University, Canberra, Australian Capital Territory, Australia
IUBMB Life 61:591-9. 2009..This ties SLC6A19 together with more complex functions such as blood-pressure control, glomerular structure, and exocytosis... - Lysinuric protein intolerance: one gene, many problemsStefan Broer
School of Biochemistry and Molecular Biology, Australian National University, Canberra, ACT, Australia
Am J Physiol Cell Physiol 293:C540-1. 2007 - The SLC6 orphans are forming a family of amino acid transportersStefan Broer
School of Biochemistry and Molecular Biology, Building 41, Australian National University, Canberra, ACT 0200, Australia
Neurochem Int 48:559-67. 2006..The structure elegantly explains many of the mechanistic features of the SLC6 amino acid transporters... - Neutral amino acid transport in epithelial cells and its malfunction in Hartnup disorderS Broer
School of Biochemistry and Molecular Biology, Australian National University, Canberra, ACT 0200, Australia
Biochem Soc Trans 33:233-6. 2005..One of the mutations occurs in members of the original Hartnup family described in 1956, thereby defining SLC6A19 as the 'Hartnup'-gene... - Xenopus laevis OocytesStefan Broer
Research School of Biology, Australian National University, Canberra, Australia
Methods Mol Biol 637:295-310. 2010.... - Adaptation of plasma membrane amino acid transport mechanisms to physiological demandsStefan Broer
School of Biochemistry and Molecular Biology, Faculty of Science, Australian National University, ACT 0200 Canberra, Australia
Pflugers Arch 444:457-66. 2002..Transport mechanisms are not conserved within families and may change with mutation of even a single amino acid residue in the transport protein. Thus transport mechanisms are easily adapted to physiological demands during evolution... - Structure-function relationships of heterodimeric amino acid transportersStefan Broer
School of Biochemistry and Molecular Biology, Australian National University, Canberra, ACT
Cell Biochem Biophys 36:155-68. 2002..A structural model of the rbAT extracellular domain is presented that is in agreement with experimental observations from several mutations and that aligns well with the alpha-amylase domain... - Further evidence for allelic heterogeneity in Hartnup disorderDimitar N Azmanov
Medical Genetics Research Unit, Australian National University ANU Medical School, Canberra, Australian Capital Territory ACT, Australia
Hum Mutat 29:1217-21. 2008..This study resolves the previous hypothesis that other genes contribute to the Hartnup phenotype... 
Regulation of the glutamine transporter SN1 by extracellular pH and intracellular sodium ionsAngelika Bröer
School of Biochemistry and Molecular Biology, Australian National University, Canberra ACT 0200, Australia
J Physiol 539:3-14. 2002..A slippage mode in the transporter mechanism and pH-regulated endogenous oocyte cation channels are likely to contribute to the observed currents...- A protein complex in the brush-border membrane explains a Hartnup disorder alleleSonja Kowalczuk
School of Biochemistry and Molecular Biology, Australian National University, Linnaeus Way 41, Canberra, ACT 0200, Australia
FASEB J 22:2880-7. 2008..This would result in reduced surface expression in both kidney and intestine, thereby explaining the onset of the disorder in individuals carrying this mutation... - Molecular cloning of mouse amino acid transport system B0, a neutral amino acid transporter related to Hartnup disorderAngelika Bröer
School of Biochemistry and Molecular Biology, Australian National University, Canberra ACT 0200, Australia
J Biol Chem 279:24467-76. 2004..Thus, the human homologue of this transporter is an excellent functional and positional candidate for Hartnup disorder... - Mutation of the 4F2 heavy-chain carboxy terminus causes y+ LAT2 light-chain dysfunctionSarah Chubb
School of Biochemistry and Molecular Biology, Australian National University, Canberra, Australia
Mol Membr Biol 23:255-67. 2006..The change of the Na+ Km caused a specific defect of leucine efflux, whereas uptake of leucine at high extracellular NaCl concentration was unaffected... - The orphan transporter v7-3 (slc6a15) is a Na+-dependent neutral amino acid transporter (B0AT2)Angelika Bröer
School of Biochemistry and Molecular Biology, Australian National University, Canberra, ACT 0200, Australia
Biochem J 393:421-30. 2006..A mouse B(0)AT2-like transport activity was detected in synaptosomes and cultured neurons. A potential role of B(0)AT2 in transporting neurotransmitter precursors and neuromodulators is proposed... - Chloroquine transport via the malaria parasite's chloroquine resistance transporterRowena E Martin
Research School of Biology, Australian National University, Canberra, Australian Capital Territory 0200, Australia
Science 325:1680-2. 2009..CQ transport via the mutant PfCRT was inhibited by CQ analogs and by the resistance-reverser verapamil. Thus, CQ resistance is due to direct transport of the drug via mutant PfCRT... - Purine uptake in Plasmodium: transport versus metabolismKiaran Kirk
Biochemistry and Molecular Biology, School of Biology, The Australian National University, Canberra ACT 0200, Australia
Trends Parasitol 25:246-9. 2009..Here, we offer an alternative interpretation of their data, suggesting that the conclusions drawn by Quashie et al. take insufficient account of metabolism... - Mutation of asparagine 76 in the center of glutamine transporter SNAT3 modulates substrate-induced conductances and Na+ bindingStefan Broer
Research School of Biology, The Australian National University, Canberra, ACT 0200, Australia
J Biol Chem 284:25823-31. 2009.... - Purine nucleobase transport in the intraerythrocytic malaria parasiteMegan J Downie
School of Biochemistry and Molecular Biology, Faculty of Science, The Australian National University, Canberra, ACT 0200, Australia
Int J Parasitol 38:203-9. 2008..The results indicate that nucleobases enter the intraerythrocytic parasite via a rapid, equilibrative process that has kinetic characteristics similar to those of PfENT1... - Molecular cloning of the mouse IMINO system: an Na+- and Cl--dependent proline transporterSonja Kowalczuk
Division of Biochemistry and Molecular Biology, Faculty of Science, Australian National University, Canberra, ACT 0200, Australia
Biochem J 386:417-22. 2005..Accordingly we suggest that the two homologues be termed 'XT3s1 IMINO(B)' and 'XT3 IMINO(K)' to indicate the tissue expression of the two genes... - Iminoglycinuria and hyperglycinuria are discrete human phenotypes resulting from complex mutations in proline and glycine transportersStefan Broer
School of Biochemistry and Molecular Biology, Australian National University, Canberra, Australian Capital Territory, Australia
J Clin Invest 118:3881-92. 2008..In summary, although recognized as apparently simple Mendelian disorders, IG and HG exhibit complex molecular explanations depending on a major gene and accompanying modifier genes... - Rapid downregulation of the rat glutamine transporter SNAT3 by a caveolin-dependent trafficking mechanism in Xenopus laevis oocytesSarojini Balkrishna
Research School of Biology, Australian National Univ, Canberra, ACT 0200, Australia
Am J Physiol Cell Physiol 299:C1047-57. 2010..Although system N activity was not affected by PMA in cultured astrocytes, a downregulation was observed in HepG2 cells... - Astroglial glutamine transport by system N is upregulated by glutamateAngelika Bröer
School of Biochemistry and Molecular Biology, Australian National University, Canberra, Australia
Glia 48:298-310. 2004..The results suggest that astrocytes respond actively to the release of glutamate by increasing glutamine release and thereby may modulate glutamatergic neurotransmission... - The molecular basis of neutral aminoaciduriasAngelika Bröer
School of Biochemistry and Molecular Biology, Australian National University, Canberra
Pflugers Arch 451:511-7. 2006..A model is presented, involving all three transporters that can explain the phenotypic variability of iminoglycinuria... - Impaired nutrient signaling and body weight control in a Na+ neutral amino acid cotransporter (Slc6a19)-deficient mouseAngelika Bröer
Research School of Biology, Australian National University, Canberra, Australian Capital Territory 0200, Australia
J Biol Chem 286:26638-51. 2011..The results demonstrate that epithelial amino acid uptake is essential for optimal growth and body weight regulation... - Sodium translocation by the iminoglycinuria associated imino transporter (SLC6A20)Angelika Bröer
Research School of Biology, Australian National University, Canberra, ACT 0200, Australia
Mol Membr Biol 26:333-46. 2009..The proposed homology model allows to study mutations in IMINO associated with iminoglycinuria... - Glutamine efflux from astrocytes is mediated by multiple pathwaysJoachim W Deitmer
School of Biochemistry and Molecular Biology, Australian National University, Canberra, Australia
J Neurochem 87:127-35. 2003.... - Intestinal peptidases form functional complexes with the neutral amino acid transporter B(0)AT1Stephen J Fairweather
Research School of Biology, Australian National University, Canberra, ACT 0200, Australia
Biochem J 446:135-48. 2012.... - Xenopus laevis OocytesStefan Broer
School of Biochemistry and Molecular Biology, Australian National University, Canberra, Australia
Methods Mol Biol 227:245-58. 2003 - Alanine metabolism, transport, and cycling in the brainStefan Broer
School of Biochemistry and Molecular Biology, Australian National University, Acton, Canberra ACT, Australia
J Neurochem 102:1758-70. 2007..Our results show that system L isoform LAT2 is critical for alanine uptake into astrocytes. However, alanine does not provide any significant carbon for energy or neurotransmitter metabolism under the conditions studied... - The solute carrier 6 family of transportersStefan Broer
Research School of Biology, Australian National University, Canberra, ACT, Australia
Br J Pharmacol 167:256-78. 2012..This review provides an overview of the biochemical and pharmacological properties of the SLC6 family transporters... - Transport of nucleosides across the Plasmodium falciparum parasite plasma membrane has characteristics of PfENT1Megan J Downie
School of Biochemistry and Molecular Biology, The Australian National University, Canberra ACT 0200, Australia
Mol Microbiol 60:738-48. 2006..The data are consistent with PfENT1 serving as a major route for the uptake of nucleosides across the parasite plasma membrane... - The loop between helix 4 and helix 5 in the monocarboxylate transporter MCT1 is important for substrate selection and protein stabilitySandra Galic
School of Biochemistry and Molecular Biology, Australian National University, Canberra ACT 0200, Australia
Biochem J 376:413-22. 2003..In contrast with MCT1(R143H), MCT1(K142Q) was less stereoselective than the wild-type. A mechanism is proposed that includes all critical residues... - Sodium-dependent uptake of inorganic phosphate by the intracellular malaria parasiteKevin J Saliba
School of Biochemistry and Molecular Biology, The Australian National University, Canberra ACT 0200, Australia
Nature 443:582-5. 2006..This study provides new insight into the significance of the malaria-parasite-induced alteration of the ionic composition of its host cell... - The role of amino acid transporters in inherited and acquired diseasesStefan Broer
Research School of Biology, Australian National University, Canberra, ACT 0200, Australia
Biochem J 436:193-211. 2011..The present review summarizes the involvement of amino acid transporters in these roles as illustrated by diseases resulting from transporter malfunction... - Targeting tumour cells at the entranceStefan Broer
Research School of Biology, Australian National University, Canberra, ACT 0200, Australia
Biochem J 439:e1-2. 2011..Thus drugs that reduce nutrient uptake are promising candidates for tumour therapy. Detailed understanding of tumour cell biology will allow the generation of new chemotherapeutic drugs with limited side effects...