Stefan Broer

Summary

Affiliation: Australian National University
Country: Australia

Publications

  1. pmc Intestinal peptidases form functional complexes with the neutral amino acid transporter B(0)AT1
    Stephen J Fairweather
    Research School of Biology, Australian National University, Canberra, ACT 0200, Australia
    Biochem J 446:135-48. 2012
  2. ncbi request reprint Diseases associated with general amino acid transporters of the solute carrier 6 family (SLC6)
    Stefan Broer
    Research School of Biology, Australian National University, Canberra, ACT 0200, Australia
    Curr Mol Pharmacol 6:74-87. 2013
  3. pmc The solute carrier 6 family of transporters
    Stefan Broer
    Research School of Biology, Australian National University, Canberra, ACT, Australia
    Br J Pharmacol 167:256-78. 2012
  4. doi request reprint The role of amino acid transporters in inherited and acquired diseases
    Stefan Broer
    Research School of Biology, Australian National University, Canberra, ACT 0200, Australia
    Biochem J 436:193-211. 2011
  5. pmc Impaired nutrient signaling and body weight control in a Na+ neutral amino acid cotransporter (Slc6a19)-deficient mouse
    Angelika Bröer
    Research School of Biology, Australian National University, Canberra, Australian Capital Territory 0200, Australia
    J Biol Chem 286:26638-51. 2011
  6. doi request reprint Targeting tumour cells at the entrance
    Stefan Broer
    Research School of Biology, Australian National University, Canberra, ACT 0200, Australia
    Biochem J 439:e1-2. 2011
  7. doi request reprint A protein complex in the brush-border membrane explains a Hartnup disorder allele
    Sonja Kowalczuk
    School of Biochemistry and Molecular Biology, Australian National University, Linnaeus Way 41, Canberra, ACT 0200, Australia
    FASEB J 22:2880-7. 2008
  8. ncbi request reprint Glutamine efflux from astrocytes is mediated by multiple pathways
    Joachim W Deitmer
    School of Biochemistry and Molecular Biology, Australian National University, Canberra, Australia
    J Neurochem 87:127-35. 2003
  9. doi request reprint Further evidence for allelic heterogeneity in Hartnup disorder
    Dimitar N Azmanov
    Medical Genetics Research Unit, Australian National University ANU Medical School, Canberra, Australian Capital Territory ACT, Australia
    Hum Mutat 29:1217-21. 2008
  10. pmc The orphan transporter v7-3 (slc6a15) is a Na+-dependent neutral amino acid transporter (B0AT2)
    Angelika Bröer
    School of Biochemistry and Molecular Biology, Australian National University, Canberra, ACT 0200, Australia
    Biochem J 393:421-30. 2006

Collaborators

Detail Information

Publications39

  1. pmc Intestinal peptidases form functional complexes with the neutral amino acid transporter B(0)AT1
    Stephen J Fairweather
    Research School of Biology, Australian National University, Canberra, ACT 0200, Australia
    Biochem J 446:135-48. 2012
    ....
  2. ncbi request reprint Diseases associated with general amino acid transporters of the solute carrier 6 family (SLC6)
    Stefan Broer
    Research School of Biology, Australian National University, Canberra, ACT 0200, Australia
    Curr Mol Pharmacol 6:74-87. 2013
    ..They may also play a role in complex traits such as depression, anxiety, obesity, diabetes and cancer. The review does not cover the transport of neurotransmitter amino acids. ..
  3. pmc The solute carrier 6 family of transporters
    Stefan Broer
    Research School of Biology, Australian National University, Canberra, ACT, Australia
    Br J Pharmacol 167:256-78. 2012
    ..This review provides an overview of the biochemical and pharmacological properties of the SLC6 family transporters...
  4. doi request reprint The role of amino acid transporters in inherited and acquired diseases
    Stefan Broer
    Research School of Biology, Australian National University, Canberra, ACT 0200, Australia
    Biochem J 436:193-211. 2011
    ..The present review summarizes the involvement of amino acid transporters in these roles as illustrated by diseases resulting from transporter malfunction...
  5. pmc Impaired nutrient signaling and body weight control in a Na+ neutral amino acid cotransporter (Slc6a19)-deficient mouse
    Angelika Bröer
    Research School of Biology, Australian National University, Canberra, Australian Capital Territory 0200, Australia
    J Biol Chem 286:26638-51. 2011
    ..The results demonstrate that epithelial amino acid uptake is essential for optimal growth and body weight regulation...
  6. doi request reprint Targeting tumour cells at the entrance
    Stefan Broer
    Research School of Biology, Australian National University, Canberra, ACT 0200, Australia
    Biochem J 439:e1-2. 2011
    ..Thus drugs that reduce nutrient uptake are promising candidates for tumour therapy. Detailed understanding of tumour cell biology will allow the generation of new chemotherapeutic drugs with limited side effects...
  7. doi request reprint A protein complex in the brush-border membrane explains a Hartnup disorder allele
    Sonja Kowalczuk
    School of Biochemistry and Molecular Biology, Australian National University, Linnaeus Way 41, Canberra, ACT 0200, Australia
    FASEB J 22:2880-7. 2008
    ..This would result in reduced surface expression in both kidney and intestine, thereby explaining the onset of the disorder in individuals carrying this mutation...
  8. ncbi request reprint Glutamine efflux from astrocytes is mediated by multiple pathways
    Joachim W Deitmer
    School of Biochemistry and Molecular Biology, Australian National University, Canberra, Australia
    J Neurochem 87:127-35. 2003
    ....
  9. doi request reprint Further evidence for allelic heterogeneity in Hartnup disorder
    Dimitar N Azmanov
    Medical Genetics Research Unit, Australian National University ANU Medical School, Canberra, Australian Capital Territory ACT, Australia
    Hum Mutat 29:1217-21. 2008
    ..This study resolves the previous hypothesis that other genes contribute to the Hartnup phenotype...
  10. pmc The orphan transporter v7-3 (slc6a15) is a Na+-dependent neutral amino acid transporter (B0AT2)
    Angelika Bröer
    School of Biochemistry and Molecular Biology, Australian National University, Canberra, ACT 0200, Australia
    Biochem J 393:421-30. 2006
    ..A mouse B(0)AT2-like transport activity was detected in synaptosomes and cultured neurons. A potential role of B(0)AT2 in transporting neurotransmitter precursors and neuromodulators is proposed...
  11. ncbi request reprint Mutation of the 4F2 heavy-chain carboxy terminus causes y+ LAT2 light-chain dysfunction
    Sarah Chubb
    School of Biochemistry and Molecular Biology, Australian National University, Canberra, Australia
    Mol Membr Biol 23:255-67. 2006
    ..The change of the Na+ Km caused a specific defect of leucine efflux, whereas uptake of leucine at high extracellular NaCl concentration was unaffected...
  12. pmc Regulation of the glutamine transporter SN1 by extracellular pH and intracellular sodium ions
    Angelika Bröer
    School of Biochemistry and Molecular Biology, Australian National University, Canberra ACT 0200, Australia
    J Physiol 539:3-14. 2002
    ..A slippage mode in the transporter mechanism and pH-regulated endogenous oocyte cation channels are likely to contribute to the observed currents...
  13. pmc Molecular cloning of the mouse IMINO system: an Na+- and Cl--dependent proline transporter
    Sonja Kowalczuk
    Division of Biochemistry and Molecular Biology, Faculty of Science, Australian National University, Canberra, ACT 0200, Australia
    Biochem J 386:417-22. 2005
    ..Accordingly we suggest that the two homologues be termed 'XT3s1 IMINO(B)' and 'XT3 IMINO(K)' to indicate the tissue expression of the two genes...
  14. doi request reprint Chloroquine transport via the malaria parasite's chloroquine resistance transporter
    Rowena E Martin
    Research School of Biology, Australian National University, Canberra, Australian Capital Territory 0200, Australia
    Science 325:1680-2. 2009
    ..CQ transport via the mutant PfCRT was inhibited by CQ analogs and by the resistance-reverser verapamil. Thus, CQ resistance is due to direct transport of the drug via mutant PfCRT...
  15. pmc Iminoglycinuria and hyperglycinuria are discrete human phenotypes resulting from complex mutations in proline and glycine transporters
    Stefan Broer
    School of Biochemistry and Molecular Biology, Australian National University, Canberra, Australian Capital Territory, Australia
    J Clin Invest 118:3881-92. 2008
    ..In summary, although recognized as apparently simple Mendelian disorders, IG and HG exhibit complex molecular explanations depending on a major gene and accompanying modifier genes...
  16. ncbi request reprint Molecular cloning of mouse amino acid transport system B0, a neutral amino acid transporter related to Hartnup disorder
    Angelika Bröer
    School of Biochemistry and Molecular Biology, Australian National University, Canberra ACT 0200, Australia
    J Biol Chem 279:24467-76. 2004
    ..Thus, the human homologue of this transporter is an excellent functional and positional candidate for Hartnup disorder...
  17. ncbi request reprint Asymmetry of glutamine transporters in cultured neural cells
    Tobias Heckel
    School of Biochemistry and Molecular Biology, Australian National University, Canberra, ACT 0200, Australia
    Neurochem Int 43:289-98. 2003
    ....
  18. pmc Mutation of asparagine 76 in the center of glutamine transporter SNAT3 modulates substrate-induced conductances and Na+ binding
    Stefan Broer
    Research School of Biology, The Australian National University, Canberra, ACT 0200, Australia
    J Biol Chem 284:25823-31. 2009
    ....
  19. doi request reprint Rapid downregulation of the rat glutamine transporter SNAT3 by a caveolin-dependent trafficking mechanism in Xenopus laevis oocytes
    Sarojini Balkrishna
    Research School of Biology, Australian National Univ, Canberra, ACT 0200, Australia
    Am J Physiol Cell Physiol 299:C1047-57. 2010
    ..Although system N activity was not affected by PMA in cultured astrocytes, a downregulation was observed in HepG2 cells...
  20. doi request reprint Sodium translocation by the iminoglycinuria associated imino transporter (SLC6A20)
    Angelika Bröer
    Research School of Biology, Australian National University, Canberra, ACT 0200, Australia
    Mol Membr Biol 26:333-46. 2009
    ..The proposed homology model allows to study mutations in IMINO associated with iminoglycinuria...
  21. ncbi request reprint The molecular basis of neutral aminoacidurias
    Angelika Bröer
    School of Biochemistry and Molecular Biology, Australian National University, Canberra
    Pflugers Arch 451:511-7. 2006
    ..A model is presented, involving all three transporters that can explain the phenotypic variability of iminoglycinuria...
  22. ncbi request reprint Astroglial glutamine transport by system N is upregulated by glutamate
    Angelika Bröer
    School of Biochemistry and Molecular Biology, Australian National University, Canberra, Australia
    Glia 48:298-310. 2004
    ..The results suggest that astrocytes respond actively to the release of glutamate by increasing glutamine release and thereby may modulate glutamatergic neurotransmission...
  23. doi request reprint Epithelial neutral amino acid transporters: lessons from mouse models
    Stefan Broer
    Research School of Biology, Australian National University, Canberra, Australia
    Curr Opin Nephrol Hypertens 22:539-44. 2013
    ..Mouse models have now established the crucial role of these transporters for systemic amino acid homeostasis. This review summarizes recent progress in this field...
  24. ncbi request reprint Xenopus laevis Oocytes
    Stefan Broer
    School of Biochemistry and Molecular Biology, Australian National University, Canberra, Australia
    Methods Mol Biol 227:245-58. 2003
  25. ncbi request reprint Alanine metabolism, transport, and cycling in the brain
    Stefan Broer
    School of Biochemistry and Molecular Biology, Australian National University, Acton, Canberra ACT, Australia
    J Neurochem 102:1758-70. 2007
    ..Our results show that system L isoform LAT2 is critical for alanine uptake into astrocytes. However, alanine does not provide any significant carbon for energy or neurotransmitter metabolism under the conditions studied...
  26. doi request reprint Amino acid transport across mammalian intestinal and renal epithelia
    Stefan Broer
    School of Biochemistry and Molecular Biology, Australian National University, Canberra, Australian Capital Territory, Australia
    Physiol Rev 88:249-86. 2008
    ..With very few exceptions, individual amino acids are transported by more than one transporter, providing backup capacity for absorption in the case of mutational inactivation of a transport system...
  27. pmc The loop between helix 4 and helix 5 in the monocarboxylate transporter MCT1 is important for substrate selection and protein stability
    Sandra Galic
    School of Biochemistry and Molecular Biology, Australian National University, Canberra ACT 0200, Australia
    Biochem J 376:413-22. 2003
    ..In contrast with MCT1(R143H), MCT1(K142Q) was less stereoselective than the wild-type. A mechanism is proposed that includes all critical residues...
  28. doi request reprint Xenopus laevis Oocytes
    Stefan Broer
    Research School of Biology, Australian National University, Canberra, Australia
    Methods Mol Biol 637:295-310. 2010
    ....
  29. doi request reprint The role of the neutral amino acid transporter B0AT1 (SLC6A19) in Hartnup disorder and protein nutrition
    Stefan Broer
    School of Biology, College of Medicine, Biology and Environment, Australian National University, Canberra, Australian Capital Territory, Australia
    IUBMB Life 61:591-9. 2009
    ..This ties SLC6A19 together with more complex functions such as blood-pressure control, glomerular structure, and exocytosis...
  30. doi request reprint Apical transporters for neutral amino acids: physiology and pathophysiology
    Stefan Broer
    School of Biochemistry and Molecular Biology, Australian National University, Canberra, Australia
    Physiology (Bethesda) 23:95-103. 2008
    ..Recent advances in the molecular identification of apical neutral amino acid transporters has shed a light on the molecular basis of Hartnup disorder and iminoglycinuria...
  31. ncbi request reprint Persistence of the common Hartnup disease D173N allele in populations of European origin
    Dimitar N Azmanov
    Medical Genetics Research Unit, ANU Medical School, Canberra, Australia
    Ann Hum Genet 71:755-61. 2007
    ..We postulate that survival of this allele may be a consequence of failure of the allele to completely inactivate the transport of neutral amino acids...
  32. doi request reprint Purine uptake in Plasmodium: transport versus metabolism
    Kiaran Kirk
    Biochemistry and Molecular Biology, School of Biology, The Australian National University, Canberra ACT 0200, Australia
    Trends Parasitol 25:246-9. 2009
    ..Here, we offer an alternative interpretation of their data, suggesting that the conclusions drawn by Quashie et al. take insufficient account of metabolism...
  33. ncbi request reprint Adaptation of plasma membrane amino acid transport mechanisms to physiological demands
    Stefan Broer
    School of Biochemistry and Molecular Biology, Faculty of Science, Australian National University, ACT 0200 Canberra, Australia
    Pflugers Arch 444:457-66. 2002
    ..Transport mechanisms are not conserved within families and may change with mutation of even a single amino acid residue in the transport protein. Thus transport mechanisms are easily adapted to physiological demands during evolution...
  34. pmc Enterocyte-specific regulation of the apical nutrient transporter SLC6A19 (B(0)AT1) by transcriptional and epigenetic networks
    Emrah Tümer
    From the Research School of Biology, Australian National University, Canberra, Australian Capital Territory 0200, Australia
    J Biol Chem 288:33813-23. 2013
    ..The results suggest that Slc6a19 expression in the intestine is regulated at three different levels involving promoter methylation, histone modification, and opposing transcription factors. ..
  35. ncbi request reprint Lysinuric protein intolerance: one gene, many problems
    Stefan Broer
    School of Biochemistry and Molecular Biology, Australian National University, Canberra, ACT, Australia
    Am J Physiol Cell Physiol 293:C540-1. 2007
  36. ncbi request reprint The SLC6 orphans are forming a family of amino acid transporters
    Stefan Broer
    School of Biochemistry and Molecular Biology, Building 41, Australian National University, Canberra, ACT 0200, Australia
    Neurochem Int 48:559-67. 2006
    ..The structure elegantly explains many of the mechanistic features of the SLC6 amino acid transporters...
  37. ncbi request reprint Purine nucleobase transport in the intraerythrocytic malaria parasite
    Megan J Downie
    School of Biochemistry and Molecular Biology, Faculty of Science, The Australian National University, Canberra, ACT 0200, Australia
    Int J Parasitol 38:203-9. 2008
    ..The results indicate that nucleobases enter the intraerythrocytic parasite via a rapid, equilibrative process that has kinetic characteristics similar to those of PfENT1...
  38. ncbi request reprint Expression of glutamine transporter Slc38a3 (SNAT3) during acidosis is mediated by a different mechanism than tissue-specific expression
    Sarojini Balkrishna
    Research School of Biology, Australian National University, Canberra, ACT, Australia
    Cell Physiol Biochem 33:1591-606. 2014
    ..Transcription of the glutamine transporter SNAT3 (Slc38a3) for instance is highly up-regulated in the kidney during metabolic acidosis to provide glutamine for ammonia production...
  39. ncbi request reprint Structure-function relationships of heterodimeric amino acid transporters
    Stefan Broer
    School of Biochemistry and Molecular Biology, Australian National University, Canberra, ACT
    Cell Biochem Biophys 36:155-68. 2002
    ..A structural model of the rbAT extracellular domain is presented that is in agreement with experimental observations from several mutations and that aligns well with the alpha-amylase domain...