Expression and purification of hepatitis B surface antigen S from Escherichia coli; a new simple methodMohamed S Elghanam
Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo, Egypt 11562
BMC Res Notes 5:125. 2012
..Therefore the need for an easy, rapid, low cost method to produce HBsAg is urgently needed within this setting...
- Identification of a bacteriocin and its cognate immunity factor expressed by Moraxella catarrhalis
Ahmed S Attia
Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, TX, USA
BMC Microbiol 9:207. 2009
..Bacteriocins are antimicrobial proteins and peptides ribosomally synthesized by some bacteria which can effect both intraspecies and interspecies killing...
- Moraxella catarrhalis expresses an unusual Hfq protein
Ahmed S Attia
Department of Microbiology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390 9048, USA
Infect Immun 76:2520-30. 2008
..coli mutant to methyl viologen. The use of an electrophoretic mobility shift assay showed that this M. catarrhalis Hfq protein could bind RNA derived from a gene whose expression was altered in the M. catarrhalis hfq mutant...
- A UspA2H-negative variant of Moraxella catarrhalis strain O46E has a deletion in a homopolymeric nucleotide repeat common to uspA2H genes
Wei Wang
Department of Microbiology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA
Infect Immun 75:2035-45. 2007
..This poly(A) tract was also found to be present in the uspA2H genes of other M. catarrhalis strains...
Development of a shuttle vector for Moraxella catarrhalisWei Wang
Department of Microbiology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390-9048, USA
Plasmid 55:50-7. 2006
..When these two plasmids were introduced into appropriate M. catarrhalis mutants, they complemented the phenotypic deficiency of each mutant. This is the first report of functional complementation in trans in this pathogen...
- A conserved tetranucleotide repeat is necessary for wild-type expression of the Moraxella catarrhalis UspA2 protein
Ahmed S Attia
Department of Microbiology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390-9048, USA
J Bacteriol 188:7840-52. 2006
..These data confirm that the presence of the AGAT repeats in the 5'-UTR of the uspA2 gene is necessary for both normal expression of the UspA2 protein and serum resistance...
- Binding of vitronectin by the Moraxella catarrhalis UspA2 protein interferes with late stages of the complement cascade
Ahmed S Attia
Department of Microbiology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390-9048, USA
Infect Immun 74:1597-611. 2006
..These results indicate that binding of vitronectin by UspA2 is involved in the serum resistance of M. catarrhalis; this represents the first example of vitronectin-mediated serum resistance on a microbe...
- Staphylococcus aureus fur regulates the expression of virulence factors that contribute to the pathogenesis of pneumonia
Victor J Torres
Department of Microbiology and Immunology, Vanderbilt University Medical Center, 1161 21st Ave South, Nashville, Tennessee 37232 2363, USA
Infect Immun 78:1618-28. 2010
..These findings reveal that Fur is involved in initiating a regulatory program that organizes the expression of virulence factors during the pathogenesis of S. aureus pneumonia...
