Usman Ali Ashfaq

Summary

Publications

  1. pmc HBV induced HCC: major risk factors from genetic to molecular level
    Ambreen Ayub
    Department of Bioinformatics and Biotechnology, Government College University Faisalabad GCUF, Faisalabad 38000, Pakistan
    Biomed Res Int 2013:810461. 2013
  2. pmc Development of global consensus sequence of HCV glycoproteins involved in viral entry
    Sobia Idrees
    Department of Bioinformatics and Biotechnology, Government College University GCU, Faisalabad, Pakistan
    Theor Biol Med Model 10:24. 2013
  3. pmc HCV Envelope protein 2 sequence comparison of Pakistani isolate and In-silico prediction of conserved epitopes for vaccine development
    Sobia Idrees
    Human Molecular Biology Group, Department of Bioinformatics and Biotechnology, Government College University GCU, Faisalabad, Pakistan
    J Transl Med 11:105. 2013
  4. pmc Structural analysis and epitope prediction of HCV E1 protein isolated in Pakistan: an in-silico approach
    Sobia Idrees
    Department of Bioinformatics and Biotechnology, Government College University GCU, Faisalabad, Pakistan
    Virol J 10:113. 2013
  5. pmc A brief review on dengue molecular virology, diagnosis, treatment and prevalence in Pakistan
    Sobia Idrees
    Department of Bioinformatics and Biotechnology, Government College University GCU, Faisalabad, Pakistan
    Genet Vaccines Ther 10:6. 2012
  6. pmc Rabies molecular virology, diagnosis, prevention and treatment
    Muhammad Zubair Yousaf
    Molecular Parasitology and Virology Laboratory, Department of Zoology, Kohat University of Science and Technology, Kohat, Pakistan
    Virol J 9:50. 2012
  7. pmc Hepatitis C virus to hepatocellular carcinoma
    Shah Jahan
    Department of Immunology, University of Health Sciences, Lahore, Pakistan
    Infect Agent Cancer 7:2. 2012
  8. pmc Anti-apoptotic effect of HCV core gene of genotype 3a in Huh-7 cell line
    Shah Jahan
    Centre of Excellence in Molecular Biology, University of the Punjab, Pakistan
    Virol J 8:522. 2011
  9. pmc The epidemic of HIV/AIDS in developing countries; the current scenario in Pakistan
    Muhammad Z Yousaf
    Institute of Biochemistry and Biotechnology University of Veterinary and Animal Sciences, Lahore
    Virol J 8:401. 2011
  10. pmc Effect of combined siRNA of HCV E2 gene and HCV receptors against HCV
    Shah Jahan
    Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan
    Virol J 8:295. 2011

Collaborators

Detail Information

Publications32

  1. pmc HBV induced HCC: major risk factors from genetic to molecular level
    Ambreen Ayub
    Department of Bioinformatics and Biotechnology, Government College University Faisalabad GCUF, Faisalabad 38000, Pakistan
    Biomed Res Int 2013:810461. 2013
    ..Several monogenic and polygenic risk factors are also involved in HCC development. This review summarizes the mechanism involved in HCC development and discusses some promising therapies to make HCC curative...
  2. pmc Development of global consensus sequence of HCV glycoproteins involved in viral entry
    Sobia Idrees
    Department of Bioinformatics and Biotechnology, Government College University GCU, Faisalabad, Pakistan
    Theor Biol Med Model 10:24. 2013
    ..HCV has two transmembrane glycoproteins (E1 and E2) that are involved in virus entry through viral attachment, but because of their hypervariable nature they have become difficult targets for vaccine development...
  3. pmc HCV Envelope protein 2 sequence comparison of Pakistani isolate and In-silico prediction of conserved epitopes for vaccine development
    Sobia Idrees
    Human Molecular Biology Group, Department of Bioinformatics and Biotechnology, Government College University GCU, Faisalabad, Pakistan
    J Transl Med 11:105. 2013
    ....
  4. pmc Structural analysis and epitope prediction of HCV E1 protein isolated in Pakistan: an in-silico approach
    Sobia Idrees
    Department of Bioinformatics and Biotechnology, Government College University GCU, Faisalabad, Pakistan
    Virol J 10:113. 2013
    ..Consequently, this study was designed to analyze the HCV E1 protein sequence isolated in Pakistan to find potential conserved epitopes/antigenic determinants...
  5. pmc A brief review on dengue molecular virology, diagnosis, treatment and prevalence in Pakistan
    Sobia Idrees
    Department of Bioinformatics and Biotechnology, Government College University GCU, Faisalabad, Pakistan
    Genet Vaccines Ther 10:6. 2012
    ..This review summarizes dengue molecular virology, important drug targets, prevalence in Pakistan, diagnosis, treatment and medicinal plant inhibitors against dengue...
  6. pmc Rabies molecular virology, diagnosis, prevention and treatment
    Muhammad Zubair Yousaf
    Molecular Parasitology and Virology Laboratory, Department of Zoology, Kohat University of Science and Technology, Kohat, Pakistan
    Virol J 9:50. 2012
    ..With the post-exposure preventive regimes, 327,000 people can prevent this disease annually.The current article mainly covers the genome, virology, symptoms, epidemiology, diagnostic methods, and the high risk countries around the globe...
  7. pmc Hepatitis C virus to hepatocellular carcinoma
    Shah Jahan
    Department of Immunology, University of Health Sciences, Lahore, Pakistan
    Infect Agent Cancer 7:2. 2012
    ..This Review illustrates viral and host factors which induce Oxidative stress, steatosis and leads toward HCC. It also expresses Molecular cascade which leads oxidative stress and steatosis to HCC...
  8. pmc Anti-apoptotic effect of HCV core gene of genotype 3a in Huh-7 cell line
    Shah Jahan
    Centre of Excellence in Molecular Biology, University of the Punjab, Pakistan
    Virol J 8:522. 2011
    ..We analyzed the effect of HCV core of genotype 1a and 3a on cell proliferation by MTT assay and on phosphrylation of Akt by western blotting in Huh-7 cells...
  9. pmc The epidemic of HIV/AIDS in developing countries; the current scenario in Pakistan
    Muhammad Z Yousaf
    Institute of Biochemistry and Biotechnology University of Veterinary and Animal Sciences, Lahore
    Virol J 8:401. 2011
    ....
  10. pmc Effect of combined siRNA of HCV E2 gene and HCV receptors against HCV
    Shah Jahan
    Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan
    Virol J 8:295. 2011
    ....
  11. pmc Down-regulation of IRES containing 5'UTR of HCV genotype 3a using siRNAs
    Saba Khaliq
    Applied and Functional Genomics lab, Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan
    Virol J 8:221. 2011
    ..HCV translation is mediated by a highly conserved internal ribosome entry site (IRES) within the 5'UTR region making it a relevant target for new drug development...
  12. pmc Antiviral activity of Acacia nilotica against Hepatitis C Virus in liver infected cells
    Sidra Rehman
    Division of Molecular Medicine, National Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan
    Virol J 8:220. 2011
    ..For further research, we will scrutinize the synergistic effect of active antiviral compound in combination with standard PEG INF-α and ribavirin which may be helpful in exploring further gateways for antiviral therapy against HCV...
  13. pmc Post-transcriptional inhibition of hepatitis C virus replication through small interference RNA
    Usman Ali Ashfaq
    Division of Molecular Medicine, National Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan
    Virol J 8:112. 2011
    ..This therapy can effectively clear the virus infection in only 50% of infected individuals. Hence, there is a dire need to develop antiviral agents against HCV...
  14. pmc Inhibition of HCV 3a core gene through Silymarin and its fractions
    Usman Ali Ashfaq
    Division of Molecular Medicine, National Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan
    Virol J 8:153. 2011
    ..Hepatitis C is a major health problem affecting 270 million individuals in world including Pakistan. Current treatment regimen, interferon alpha and ribavirin only cure half of patients due to side effects and high cost...
  15. pmc In-vitro model systems to study Hepatitis C Virus
    Usman Ali Ashfaq
    Division of Molecular Medicine, National Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan
    Genet Vaccines Ther 9:7. 2011
    ..This review summarizes the most important in-vitro models currently used to study future HCV research as well as drug design...
  16. pmc An overview of HCV molecular biology, replication and immune responses
    Usman A Ashfaq
    Division of Molecular Medicine, National Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan
    Virol J 8:161. 2011
    ..This advance knowledge will be helpful for development of vaccine against HCV and discovery of new medicines both from synthetic chemistry and natural sources...
  17. pmc Lysosomotropic agents as HCV entry inhibitors
    Usman A Ashfaq
    Division of Molecular Medicine, National Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan
    Virol J 8:163. 2011
    ..These results suggest that inhibition of HCV at fusion step by increasing the lysosomal pH will be better option to treat chronic HCV...
  18. pmc Inhibition of full length hepatitis C virus particles of 1a genotype through small interference RNA
    Muhammad Ansar
    Division of Molecular Medicine, National Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan
    Virol J 8:203. 2011
    ..Thus there is a great need for the development of new treatments for HCV infections. RNA interference (RNAi) represents a new promising approach to develop effective antiviral drugs and has been extremely effective against HCV infection...
  19. pmc Inhibition of HCV 3a genotype entry through host CD81 and HCV E2 antibodies
    Usman A Ashfaq
    Division of Molecular Medicine, National Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan
    J Transl Med 9:194. 2011
    ..HCV glycoproteins play an important role in HCV entry by binding with CD81 receptors. Hence inhibition of virus at entry step is an important target to identify antiviral drugs against HCV...
  20. pmc Inhibition of hepatitis C virus 3a genotype entry through Glanthus Nivalis Agglutinin
    Usman A Ashfaq
    Division of Molecular Medicine, Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan
    Virol J 8:248. 2011
    ..Glanthus Nivalis Agglutinin (GNA) is a mannose binding lectin which has tendency for specific recognition and reversible binding to the sugar moieties of a wide variety of glycoproteins of enveloped viruses...
  21. pmc Glycyrrhizin as antiviral agent against Hepatitis C Virus
    Usman A Ashfaq
    Division of Molecular Medicine, National Centre of Excellence in Molecular Biology, University of Punjab, Lahore, Pakistan
    J Transl Med 9:112. 2011
    ..Herbal plants have been used for centuries against different diseases including viral diseases and have become a major source of new compounds to treat bacterial and viral diseases...
  22. pmc siRNAs: potential therapeutic agents against hepatitis C virus
    Usman A Ashfaq
    Division of Molecular Medicine, National Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan
    Virol J 8:276. 2011
    ..In the present review, we focused that combination of siRNAs against virus and host genes will be a better option to treat HCV...
  23. pmc Antiviral drugs against hepatitis C virus
    Sidra Rehman
    Division of Molecular Medicine, National Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan
    Genet Vaccines Ther 9:11. 2011
    ..Conclusively, further innovative research on chemical compounds will lead to consistent standard therapy with fewer side effects...
  24. pmc In-vitro antiviral activity of Solanum nigrum against Hepatitis C Virus
    Tariq Javed
    Division of Molecular Medicine, National Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan
    Virol J 8:26. 2011
    ..Hence, there is a need for the development of more effective, less toxic antiviral agents...
  25. doi request reprint RNAi as a new therapeutic strategy against HCV
    Saba Khaliq
    Functional and Applied Genomics Laboratory, National Center of Excellence in Molecular Biology, University of the Punjab, Lahore 53700, Pakistan
    Biotechnol Adv 28:27-34. 2010
    ..In the present review, we focused on the recent development in the potential use and issues regarding siRNA as a therapy for HCV...
  26. pmc NS4A protein as a marker of HCV history suggests that different HCV genotypes originally evolved from genotype 1b
    Muhammad T Sarwar
    Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan
    Virol J 8:317. 2011
    ..HCV genome codes for a single polyprotein of about 3011 amino acids which is processed into structural and non-structural proteins inside host cell by viral and cellular proteases...
  27. doi request reprint Hepatitis C virus entry: role of host and viral factors
    Baila Samreen
    National Center of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan
    Infect Genet Evol 12:1699-709. 2012
    ..In the current review we try to summarize the current knowledge about mechanism and interaction of cellular and viral factors involved in HCV cell entry and its implication as therapeutic target to inhibit HCV infection...
  28. ncbi request reprint Gene expression profiling of immune responsive and fibrosis genes in hepatitis C virus infected patients
    Sobia Idrees
    1 Department of Bioinformatics and Biotechnology, Government College University, Faisalabad, Pakistan
    Viral Immunol 27:250-4. 2014
    ..The information gained through this study is helpful to identify a noninvasive marker to determine liver fibrosis, and may also give useful information to understand HCV pathogenesis and develop better therapeutic regimens. ..