Takayuki Asahara

Summary

Publications

  1. ncbi request reprint Bone marrow origin of endothelial progenitor cells responsible for postnatal vasculogenesis in physiological and pathological neovascularization
    T Asahara
    Department of Medicine, St Elizabeth s Medical Center, Tufts University School of Medicine, Boston, MA, USA
    Circ Res 85:221-8. 1999
  2. ncbi request reprint Bone marrow as a source of endothelial cells for natural and iatrogenic vascular repair
    J M Isner
    Department of Medicine, St Elizabeth s Medical Center, Tufts University School of Medicine, Boston, Massachusetts 02135, USA
    Ann N Y Acad Sci 953:75-84. 2001
  3. ncbi request reprint Endothelial progenitor cells for postnatal vasculogenesis
    Takayuki Asahara
    Stem Cell Translational Research, Kobe Institute of Biomedical Research and Innovation RIKEN Center for Developmental Biology, 2 2 Minatojima Minamimachi, Chuo Ku, Kobe 650 0047, Japan
    Am J Physiol Cell Physiol 287:C572-9. 2004
  4. ncbi request reprint Endothelial progenitor cells for vascular regeneration
    Takayuki Asahara
    Cardiovascular Research and Medicine, St Elizabeth s Medical Center, Tufts University School of Medicine, Boston, MA 02135, USA
    J Hematother Stem Cell Res 11:171-8. 2002
  5. ncbi request reprint [Endothelial progenitor cells for vascular medicine]
    Takayuki Asahara
    Regenerative Medicine and Research, Kobe Institute of Biomedical Research and Innovation RIKEN Center for Developmental Biology, Japan
    Yakugaku Zasshi 127:841-5. 2007
  6. doi request reprint Local delivery of granulocyte colony stimulating factor-mobilized CD34-positive progenitor cells using bioscaffold for modality of unhealing bone fracture
    Yutaka Mifune
    Stem Cell Translational Research, Institute of Biomedical Research and Innovation RIKEN Center for Developmental Biology, Kobe, Japan
    Stem Cells 26:1395-405. 2008
  7. ncbi request reprint Endothelial progenitor cells are rapidly recruited to myocardium and mediate protective effect of ischemic preconditioning via "imported" nitric oxide synthase activity
    Masaaki Ii
    Division of Cardiovascular Research, Caritas St Elizabeth s Medical Center, Tufts University School of Medicine, Boston, Mass 02135, USA
    Circulation 111:1114-20. 2005
  8. ncbi request reprint Stromal cell-derived factor-1 effects on ex vivo expanded endothelial progenitor cell recruitment for ischemic neovascularization
    Jun Ichi Yamaguchi
    Division of Cardiovascular Research and Medicine, St Elizabeth s Medical Center, Tufts University School of Medicine, Boston, Mass 02135, USA
    Circulation 107:1322-8. 2003
  9. ncbi request reprint Synchrotron radiation coronary microangiography for morphometric and physiological evaluation of myocardial neovascularization induced by endothelial progenitor cell transplantation
    Hiroto Iwasaki
    Stem Cell Translational Research, Kobe Institute of Biomedical Research and Innovation RIKEN Center for Developmental Biology, 2 2 Minatojima Minamimachi, Chuo Ku, Kobe 650 0047, Japan
    Arterioscler Thromb Vasc Biol 27:1326-33. 2007
  10. ncbi request reprint Tumor necrosis factor-alpha receptor p75 is required in ischemia-induced neovascularization
    David A Goukassian
    Division of Cardiovascular Diseases, Department of Medicine, Caritas St Elizabeth s Medical Center, Boston, Mass, USA
    Circulation 115:752-62. 2007

Research Grants

  1. POSTNATAL VASCULOGENESIS IN CARDIOVASCULAR DISEASE
    Takayuki Asahara; Fiscal Year: 2001

Detail Information

Publications80

  1. ncbi request reprint Bone marrow origin of endothelial progenitor cells responsible for postnatal vasculogenesis in physiological and pathological neovascularization
    T Asahara
    Department of Medicine, St Elizabeth s Medical Center, Tufts University School of Medicine, Boston, MA, USA
    Circ Res 85:221-8. 1999
    ....
  2. ncbi request reprint Bone marrow as a source of endothelial cells for natural and iatrogenic vascular repair
    J M Isner
    Department of Medicine, St Elizabeth s Medical Center, Tufts University School of Medicine, Boston, Massachusetts 02135, USA
    Ann N Y Acad Sci 953:75-84. 2001
    ..Likewise, augmented or retarded neovascularization probably involves enhancement or impariment of the vasculogenesis process...
  3. ncbi request reprint Endothelial progenitor cells for postnatal vasculogenesis
    Takayuki Asahara
    Stem Cell Translational Research, Kobe Institute of Biomedical Research and Innovation RIKEN Center for Developmental Biology, 2 2 Minatojima Minamimachi, Chuo Ku, Kobe 650 0047, Japan
    Am J Physiol Cell Physiol 287:C572-9. 2004
    ..In this review, we summarize biological features of EPCs and speculate on the utility of EPCs for vascular and general medicine...
  4. ncbi request reprint Endothelial progenitor cells for vascular regeneration
    Takayuki Asahara
    Cardiovascular Research and Medicine, St Elizabeth s Medical Center, Tufts University School of Medicine, Boston, MA 02135, USA
    J Hematother Stem Cell Res 11:171-8. 2002
    ..This review discusses the therapeutic potential of EPCs for cardiovascular ischemic diseases...
  5. ncbi request reprint [Endothelial progenitor cells for vascular medicine]
    Takayuki Asahara
    Regenerative Medicine and Research, Kobe Institute of Biomedical Research and Innovation RIKEN Center for Developmental Biology, Japan
    Yakugaku Zasshi 127:841-5. 2007
    ..The possibility that modulation of vasculogenesis can be used therapeutically to augment as well as inhibit neovascularization deserves further investigation...
  6. doi request reprint Local delivery of granulocyte colony stimulating factor-mobilized CD34-positive progenitor cells using bioscaffold for modality of unhealing bone fracture
    Yutaka Mifune
    Stem Cell Translational Research, Institute of Biomedical Research and Innovation RIKEN Center for Developmental Biology, Kobe, Japan
    Stem Cells 26:1395-405. 2008
    ..Disclosure of potential conflicts of interest is found at the end of this article...
  7. ncbi request reprint Endothelial progenitor cells are rapidly recruited to myocardium and mediate protective effect of ischemic preconditioning via "imported" nitric oxide synthase activity
    Masaaki Ii
    Division of Cardiovascular Research, Caritas St Elizabeth s Medical Center, Tufts University School of Medicine, Boston, Mass 02135, USA
    Circulation 111:1114-20. 2005
    ..The possibility that EPCs could act as reservoirs of cytokines has been implied by several observations; however, a specific role for cytokine delivery has not been identified...
  8. ncbi request reprint Stromal cell-derived factor-1 effects on ex vivo expanded endothelial progenitor cell recruitment for ischemic neovascularization
    Jun Ichi Yamaguchi
    Division of Cardiovascular Research and Medicine, St Elizabeth s Medical Center, Tufts University School of Medicine, Boston, Mass 02135, USA
    Circulation 107:1322-8. 2003
    ..Given the close relationship between hematopoietic stem cells and endothelial progenitor cells (EPCs), we investigated the effect of SDF-1 on EPC-mediated vasculogenesis...
  9. ncbi request reprint Synchrotron radiation coronary microangiography for morphometric and physiological evaluation of myocardial neovascularization induced by endothelial progenitor cell transplantation
    Hiroto Iwasaki
    Stem Cell Translational Research, Kobe Institute of Biomedical Research and Innovation RIKEN Center for Developmental Biology, 2 2 Minatojima Minamimachi, Chuo Ku, Kobe 650 0047, Japan
    Arterioscler Thromb Vasc Biol 27:1326-33. 2007
    ..However, it has been technically difficult to obtain imaging evidence of collateral formation by conventional angiography...
  10. ncbi request reprint Tumor necrosis factor-alpha receptor p75 is required in ischemia-induced neovascularization
    David A Goukassian
    Division of Cardiovascular Diseases, Department of Medicine, Caritas St Elizabeth s Medical Center, Boston, Mass, USA
    Circulation 115:752-62. 2007
    ..Little is known about the role of TNF-alpha receptors (TNFR1/p55 and TNFR2/p75) in angiogenic signaling...
  11. ncbi request reprint CD34-positive cells exhibit increased potency and safety for therapeutic neovascularization after myocardial infarction compared with total mononuclear cells
    Atsuhiko Kawamoto
    Division of Cardiovascular Research, St Elizabeth s Medical Center, Tufts University School of Medicine, Boston, Mass 02135, USA
    Circulation 114:2163-9. 2006
    ..We compared the therapeutic potential of purified mobilized human CD34+ cells with that of mobilized total mononuclear cells (tMNCs) for the preservation/recovery of myocardial tissue integrity and function after myocardial infarction (MI)...
  12. doi request reprint Ex-vivo expanded human blood-derived CD133+ cells promote repair of injured spinal cord
    Naosuke Kamei
    Group of Vascular Regeneration, Institute of Biomedical Research and Innovation, 2 2 Minatojima Minamimachi, Kobe, Hyogo, 650 0047, Japan
    J Neurol Sci 328:41-50. 2013
    ..In addition, high-dose administration of expanded cells was highly effective in the induction of regenerative processes at the injured spinal cord...
  13. ncbi request reprint Dose-dependent contribution of CD34-positive cell transplantation to concurrent vasculogenesis and cardiomyogenesis for functional regenerative recovery after myocardial infarction
    Hiroto Iwasaki
    Stem Cell Translational Research, Kobe Institute of Biomedical Research and Innovation RIKEN Center for Developmental Biology, Kobe, Japan
    Circulation 113:1311-25. 2006
    ..In the present study we performed a series of experiments to prove our hypothesis that vasculogenesis and cardiomyogenesis after myocardial infarction (MI) may be dose-dependently enhanced after CD34+ cell transplantation...
  14. doi request reprint Fracture induced mobilization and incorporation of bone marrow-derived endothelial progenitor cells for bone healing
    Tomoyuki Matsumoto
    Stem Cell Translational Research, Kobe Institute of Biomedical Research and Innovation RIKEN Center for Developmental Biology, Kobe, Japan
    J Cell Physiol 215:234-42. 2008
    ..EPCs may play an essential role in fracture healing by promoting a favorable environment through neovascularization in damaged skeletal tissue...
  15. pmc PlGF repairs myocardial ischemia through mechanisms of angiogenesis, cardioprotection and recruitment of myo-angiogenic competent marrow progenitors
    Hiroto Iwasaki
    Stem Cell Translational Research, Institute of Biomedical Research and Innovation RIKEN Center for Developmental Biology, Kobe, Japan
    PLoS ONE 6:e24872. 2011
    ..Despite preclinical success in regenerating and revascularizing the infarcted heart using angiogenic growth factors or bone marrow (BM) cells, recent clinical trials have revealed less benefit from these therapies than expected...
  16. ncbi request reprint Estradiol enhances recovery after myocardial infarction by augmenting incorporation of bone marrow-derived endothelial progenitor cells into sites of ischemia-induced neovascularization via endothelial nitric oxide synthase-mediated activation of matrix m
    Atsushi Iwakura
    Division of Cardiovascular Research, St Elizabeth s Medical Center, Tufts University School of Medicine, Boston, MA, USA
    Circulation 113:1605-14. 2006
    ..We hypothesized that estradiol could augment the incorporation of bone marrow (BM)-derived EPCs into sites of ischemia-induced neovascularization, resulting in protection from ischemic injury...
  17. doi request reprint Concurrent vasculogenesis and neurogenesis from adult neural stem cells
    Masaaki Ii
    Group of Vascular Regeneration Research, Institute of Biomedical Research and Innovation, 2 2, Minatojima Minamimachi, Chuo Ku, Kobe 650 0047, Japan
    Circ Res 105:860-8. 2009
    ..Recent reports have demonstrated that signals from vascular endothelial cells are necessary for organogenesis that may precede vasculogenesis. However, the origin of these neovascular cells in regenerating tissue has not been clarified...
  18. ncbi request reprint Hyperhomocyst(e)inemia impairs angiogenesis in a murine model of limb ischemia
    Marta Bosch-Marce
    Department of Medicine Cardiovascular Research, Caritas St Elizabeth s Medical Center, Tufts University School of Medicine, Boston, MA 02135 2997, USA
    Vasc Med 10:15-22. 2005
    ..In contrast, our data suggest that impaired Akt phosphorylation mediates the impairment of angiogenesis associated with HH...
  19. ncbi request reprint Constitutive human telomerase reverse transcriptase expression enhances regenerative properties of endothelial progenitor cells
    Satoshi Murasawa
    Department of Medicine Vascular Medicine, Cardiology, Cardiovascular Research, St Elizabeth s Medical Center, Tufts University School of Medicine, Boston, Mass 02135, USA
    Circulation 106:1133-9. 2002
    ....
  20. ncbi request reprint Estrogen-mediated, endothelial nitric oxide synthase-dependent mobilization of bone marrow-derived endothelial progenitor cells contributes to reendothelialization after arterial injury
    Atsushi Iwakura
    Division of Cardiovascular Research, St Elizabeth s Medical Center, Tufts University School of Medicine, Boston, Mass 02135, USA
    Circulation 108:3115-21. 2003
    ..We hypothesized that estrogen-induced acceleration of reendothelialization might be mediated in part by effects involving mobilization and incorporation of bone marrow-derived endothelial progenitor cells (EPCs)...
  21. doi request reprint Administrations of peripheral blood CD34-positive cells contribute to medial collateral ligament healing via vasculogenesis
    Katsumasa Tei
    Department of Orthopaedic Surgery, Kobe University Graduate School of Medicine 7 5 1, Kusunoki cho, Chuo Ku, Kobe 650 0017, Japan
    Stem Cells 26:819-30. 2008
    ..Our data strongly suggest that local transplantation of circulating human CD34+ cells may augment the ligament healing process by promoting a favorable environment through neovascularization...
  22. ncbi request reprint Determination of bone marrow-derived endothelial progenitor cell significance in angiogenic growth factor-induced neovascularization in vivo
    Toshinori Murayama
    Division of Cardiovascular Research, Department of Medicine, St Elizabeth s Medical Center of Boston, Tufts University School of Medicine, Brighton, Mass 02135 2997, USA
    Exp Hematol 30:967-72. 2002
    ..To address this issue, we investigated the quantitative contribution of EPCs to newly formed vascular structures in an in vivo Matrigel plug assay and corneal micropocket assay...
  23. doi request reprint Therapeutic superiority for cartilage repair by CD271-positive marrow stromal cell transplantation
    Yutaka Mifune
    Stem Cell Translational Research, Kobe Institute of Biomedical Research and Innovation, Kobe, Japan
    Cell Transplant 22:1201-11. 2013
    ..These results indicate that CD271+ MSCs have a greater chondrogenic potential than PA-MSCs in both in vitro and in vivo conditions...
  24. pmc Therapeutic potential of vasculogenesis and osteogenesis promoted by peripheral blood CD34-positive cells for functional bone healing
    Tomoyuki Matsumoto
    Stem Cell Translational Research, Kobe Institute of Biomedical Research and Innovation RIKEN Center for Developmental Biology, 2 2 Minatojima Minamimachi, Chuo Ku, Kobe 650 0047
    Am J Pathol 169:1440-57. 2006
    ..Our data suggest circulating human CD34+ cells have therapeutic potential to promote an environment conducive to neovascularization and osteogenesis in damaged skeletal tissue, allowing the complete healing of fractures...
  25. pmc Functional disruption of alpha4 integrin mobilizes bone marrow-derived endothelial progenitors and augments ischemic neovascularization
    Gangjian Qin
    Cardiovascular Research, Caritas St Elizabeth s Medical Center, Tufts University School of Medicine, Boston, MA 02135
    J Exp Med 203:153-63. 2006
    ..These data indicate that functional disruption of alpha4 integrin may represent a potential angiogenic therapy for ischemic disease by increasing the available circulating supply of EPCs...
  26. ncbi request reprint Isolation of cardiac cells from E8.5 yolk sac by ALCAM (CD166) expression
    Yoshinobu Murakami
    Foundation of Biomedical Research and Innovation, 2 2 Minatojima Minamimachi, Chuo Ku, Kobe 650 0047, Japan
    Mech Dev 124:830-9. 2007
    ..5 yolk sacs and can be isolated as ALCAM high+, Flk-1- cells. Our report provides novel insights into the origin and differentiation process of cardiac cells in the formation of the circulatory system...
  27. ncbi request reprint Magnetic resonance mapping of transplanted endothelial progenitor cells for therapeutic neovascularization in ischemic heart disease
    Alberto Weber
    Divisions of Cardiovascular Research and Cardiovascular Medicine, St Elizabeth s Medical Center, Tufts University School of Medicine, Boston, MA 02135, USA
    Eur J Cardiothorac Surg 26:137-43. 2004
    ..We present a novel technique using magnetic resonance imaging (MRI) to localize transplanted EPCs in ischemic hearts...
  28. ncbi request reprint Intramyocardial transplantation of autologous endothelial progenitor cells for therapeutic neovascularization of myocardial ischemia
    Atsuhiko Kawamoto
    Division of Cardiovascular Research, St Elizabeth s Medical Center, Tufts University School of Medicine, Boston, Mass 02135, USA
    Circulation 107:461-8. 2003
    ..We investigated whether catheter-based, intramyocardial transplantation of autologous endothelial progenitor cells can enhance neovascularization in myocardial ischemia...
  29. ncbi request reprint Neuronal nitric oxide synthase mediates statin-induced restoration of vasa nervorum and reversal of diabetic neuropathy
    Masaaki Ii
    Division of Cardiovascular Research, Caritas St Elizabeth s Medical Center, Tufts University School of Medicine, 736 Cambridge St, Boston, MA 02135, USA
    Circulation 112:93-102. 2005
    ..Peripheral neuropathy is a frequent and major complication of diabetes...
  30. doi request reprint Therapeutic potential of unrestricted somatic stem cells isolated from placental cord blood for cardiac repair post myocardial infarction
    Hiroto Iwasaki
    Stem Cell Translational Research, Institute of Biomedical Research and Innovation RIKEN Center for Developmental Biology, Chuo Ku, Kobe, Japan
    Arterioscler Thromb Vasc Biol 29:1830-5. 2009
    ..Unrestricted somatic stem cells (USSCs) were successfully identified from human cord blood. However, the efficacy of USSC transplantation for improving left ventricular (LV) function post myocardial infarction (MI) is still controversial...
  31. doi request reprint Lnk deletion reinforces the function of bone marrow progenitors in promoting neovascularization and astrogliosis following spinal cord injury
    Naosuke Kamei
    Group of Vascular Regeneration, Institute of Biomedical Research and Innovation, 2 2 Minatojima Minamimachi, Chuo Ku, Kobe, Hyogo 650 0047, Japan
    Stem Cells 28:365-75. 2010
    ..These findings suggest that Lnk deletion reinforces the commitment of KSL cells to EPCs, promoting subsequent repair of injured spinal cord through the acceleration of angiogenesis and astrogliosis...
  32. ncbi request reprint Transplantation of endothelial progenitor cells for therapeutic neovascularization
    Atsuhiko Kawamoto
    Division of Cardiovascular Research, St Elizabeth s Medical Center, Tufts University School of Medicine, 736 Cambridge Street, Boston, MA, USA
    Cardiovasc Radiat Med 3:221-5. 2002
    ..Further basic research, with improved understanding of the mechanisms governing homing and incorporation of EPCs, will be still necessary to optimize the methodology of the cell therapy...
  33. ncbi request reprint Functional ephrin-B2 expression for promotive interaction between arterial and venous vessels in postnatal neovascularization
    Shin Ichiro Hayashi
    Division of Cardiovascular Research and Medicine, St Elizabeth s Medical Center, Tufts University School of Medicine, Boston, Mass 02135, USA
    Circulation 111:2210-8. 2005
    ..In this study, we focused on the function of ephrin-B2 in postnatal neovascularization...
  34. doi request reprint Intramuscular transplantation of G-CSF-mobilized CD34(+) cells in patients with critical limb ischemia: a phase I/IIa, multicenter, single-blinded, dose-escalation clinical trial
    Atsuhiko Kawamoto
    Division of Vascular Regeneration Therapy, Department of Translational Research, Institute of Biomedical Research and Innovation, Kobe 650 0047, Japan
    Stem Cells 27:2857-64. 2009
    ..Favorable trends in efficacy parameters encourage a randomized and controlled trial in the future...
  35. pmc Clonally expanded novel multipotent stem cells from human bone marrow regenerate myocardium after myocardial infarction
    Young Sup Yoon
    Division of Cardiovascular Medicine, Caritas St Elizabeth s Medical Center, Boston, Massachusetts 02135, USA
    J Clin Invest 115:326-38. 2005
    ..To our knowledge, this is the first demonstration that a specific population of multipotent human BM-derived stem cells can induce both therapeutic neovascularization and endogenous and exogenous cardiomyogenesis...
  36. ncbi request reprint Statin therapy accelerates reendothelialization: a novel effect involving mobilization and incorporation of bone marrow-derived endothelial progenitor cells
    Dirk H Walter
    Department of Medicine Cardiovascular Research, St Elizabeth s Medical Center, Tufts University School of Medicine, Boston, Mass 02135, USA
    Circulation 105:3017-24. 2002
    ..Primary and secondary prevention trials suggest that statins possess favorable effects independent of cholesterol reduction. We investigated whether statin therapy may also accelerate reendothelialization after carotid balloon injury...
  37. ncbi request reprint Engineering the response to vascular injury: divergent effects of deregulated E2F1 expression on vascular smooth muscle cells and endothelial cells result in endothelial recovery and inhibition of neointimal growth
    David A Goukassian
    Department of Medicine, Division of Cardiovascular Research, St Elizabeth s Medical Center, Boston, Mass, USA
    Circ Res 93:162-9. 2003
    ....
  38. ncbi request reprint Sonic hedgehog induces arteriogenesis in diabetic vasa nervorum and restores function in diabetic neuropathy
    Kengo F Kusano
    Division of Cardiovascular Research, St Elizabeth Medical Center Boston, Boston, MA 02135, USA
    Arterioscler Thromb Vasc Biol 24:2102-7. 2004
    ..Accordingly, we investigated the hypothesis that systemic injection of SHh protein could improve nerve blood flow and function in diabetic neuropathy (DN)...
  39. ncbi request reprint Phase 1/2 placebo-controlled, double-blind, dose-escalating trial of myocardial vascular endothelial growth factor 2 gene transfer by catheter delivery in patients with chronic myocardial ischemia
    Douglas W Losordo
    Division of Cardiology, Tufts School of Medicine St Elizabeth s Medical Center, Boston, Mass 02135, USA
    Circulation 105:2012-8. 2002
    ....
  40. doi request reprint Local transplantation of G-CSF-mobilized CD34(+) cells in a patient with tibial nonunion: a case report
    Ryosuke Kuroda
    Department of Orthopedic Surgery, Kobe University Graduate School of Medicine, Chuo Ku, Kobe, Japan
    Cell Transplant 20:1491-6. 2011
    ..No serious adverse event occurred, and the novel therapy performed 9 months after the primary operation resulted in bone union 3 months later without any symptoms including pain and gait disturbance...
  41. ncbi request reprint Coexpression of platelet-derived growth factor receptor alpha and fetal liver kinase 1 enhances cardiogenic potential in embryonic stem cell differentiation in vitro
    Hirokazu Hirata
    Department of Tissue Engineering and Cell Therapy, Foundation of Biomedical Research and Innovation, 2 2 Minatojima Minamimachi, Kobe, Japan
    J Biosci Bioeng 103:412-9. 2007
    ....
  42. ncbi request reprint Endothelial progenitor cell vascular endothelial growth factor gene transfer for vascular regeneration
    Hideki Iwaguro
    Division of Cardiovascular Research and Medicine, St Elizabeth s Medical Center, Tufts University School of Medicine, Boston, Mass 02135, USA
    Circulation 105:732-8. 2002
    ..In the current study, we investigated the hypothesis that gene transfer can be used to achieve phenotypic modulation of EPCs...
  43. doi request reprint Up-regulation of miR-210 by vascular endothelial growth factor in ex vivo expanded CD34+ cells enhances cell-mediated angiogenesis
    Mohamad Amer Alaiti
    Department of Medicine, Case Cardiovascular Research Institute, University Hospital Case Medical Center, Case Western Reserve University School of Medicine, Cleveland, OH 44106 5038, USA
    J Cell Mol Med 16:2413-21. 2012
    ....
  44. doi request reprint CD133+ endothelial progenitor cells as a potential cell source for a bioartificial glomerulus
    Duc M Vu
    Division of Nephrology and Metabolism, Department of Medicine, Tokai University School of Medicine, Kanagawa, Japan
    Tissue Eng Part A 15:3173-82. 2009
    ..These results suggest that CD133(+) EPCs would potentially be applicable in bioartificial glomerulus...
  45. doi request reprint Human peripheral blood CD34-positive cells enhance therapeutic regeneration of chronically injured liver in nude rats
    Toru Nakamura
    Division of Gastroenterology, Department of Medicine, Kurume University School of Medicine, Kurume, Fukuoka, Japan
    J Cell Physiol 227:1538-52. 2012
    ..We conclude that human peripheral blood CD34(+) cell transplantation halts established liver fibrosis and promotes hepatic regeneration in CCl(4)-induced chronic liver injury...
  46. ncbi request reprint Post-natal endothelial progenitor cells for neovascularization in tissue regeneration
    Haruchika Masuda
    Department of Physiology, Tokai University School of Medicine, Bohseidai, Isehara, 259 1193, Kanagawa, Japan
    Cardiovasc Res 58:390-8. 2003
    ..This review provides an update of EPC biology as well as highlighting their potential use for therapeutic regeneration...
  47. doi request reprint Cardiogenic potential of endothelial progenitor cells
    Satoshi Murasawa
    Group of Vascular Regeneration, Institute of Biomedical Research and Innovation, Kobe, Japan
    Ther Adv Cardiovasc Dis 2:341-8. 2008
    ..Recent publications from our own and other groups have reported the possibility of cardiogenic potential of EPCs. We would like to focus on the strategies of EPC transplantation and cardiomyogenesis of EPCs in this review...
  48. ncbi request reprint Depletion of endothelial progenitor cells in the peripheral blood of patients with ulcerative colitis
    Junya Masuda
    Division of Gastroenterology, Department of Medicine, Kurume University School of Medicine, 67 Asahi Machi, Kurume, Fukuoka 830 0011, Japan
    Int J Mol Med 19:221-8. 2007
    ..Further studies are needed to define the mechanisms that underlie the reduction in the number of circulating EPCs and to better understand the pathophysiological consequences of this event in patients with UC...
  49. ncbi request reprint p53-induced inhibition of Hif-1 causes cardiac dysfunction during pressure overload
    Masanori Sano
    Department of Cardiovascular Science and Medicine, Chiba University Graduate School of Medicine, 1 8 1 Inohana, Chuo Ku, Chiba 260 8670, Japan
    Nature 446:444-8. 2007
    ..These results indicate that the anti-angiogenic property of p53 may have a crucial function in the transition from cardiac hypertrophy to heart failure...
  50. ncbi request reprint [Vascular regeneration]
    Taro Shoji
    Group of Vascular Regeneration Research, Institute of Biomedical Research and Innovation
    Nihon Rinsho 66:899-907. 2008
    ..On the basis of the regenerative potency, these stem/progenitor cells are expected as a key factor of therapeutic applications for the ischemic diseases...
  51. doi request reprint CD133+ cells from human peripheral blood promote corticospinal axon regeneration
    Risako Yamamoto
    Department of Orthopaedic Surgery, Graduate School of Biomedical Sciences, Hiroshima University, Japan
    Neuroreport 19:799-803. 2008
    ..In contrast, the transplanted cells did not differentiate into endothelial cells. These data suggest that CD133 cells may promote axonal regeneration by upregulating vascular endothelial growth factor mRNA in spinal cord tissues...
  52. doi request reprint Specific Jagged-1 signal from bone marrow microenvironment is required for endothelial progenitor cell development for neovascularization
    Sang Mo Kwon
    Stem Cell Translational Research Laboratory, RIKEN Center For Developmental Biology Institute of Biomedical Research and Innovation, Kobe, Japan
    Circulation 118:157-65. 2008
    ..Despite accumulating evidence that proves the pivotal role of endothelial progenitor cells (EPCs) in ischemic neovascularization, the key signaling cascade that regulates functional EPC kinetics remains unclear...
  53. doi request reprint Circulating endothelial/skeletal progenitor cells for bone regeneration and healing
    Tomoyuki Matsumoto
    Department of Orthopaedic Surgery, Kobe University Graduate School of Medicine, Japan
    Bone 43:434-9. 2008
    ..This review will also highlight current concepts of circulating stem/progenitor cell-based therapy and their potential application for bone repair...
  54. ncbi request reprint Adult reserve stem cells and their potential for tissue engineering
    Henry E Young
    Division of Basic Medical Sciences, Mercer University School of Medicine, Macon, GA, USA
    Cell Biochem Biophys 40:1-80. 2004
    ..Several model systems are currently being investigated to determine the possibilities of using these adult quiescent reserve precursor cells for tissue engineering...
  55. doi request reprint The effects of flap ischemia on normal and diabetic progenitor cell function
    Rica Tanaka
    Division of Regenerative Medicine, Department of Basic Clinical Science, Tokai University School of Medicine, Tokyo, Japan
    Plast Reconstr Surg 121:1929-42. 2008
    ..This is the first investigation into the effects of flap ischemia on circulating and bone marrow-derived endothelial progenitor cells. Potential mechanisms for impaired vasculogenesis in diabetes are also investigated...
  56. doi request reprint Tracking adult neovascularization during ischemia and inflammation using Vegfr2-LacZ reporter mice
    Regina Heidenreich
    Department of Dermatology, Eberhard Karls University, Tuebingen, Germany
    J Vasc Res 45:437-44. 2008
    ..Moreover, the Vegfr2/LacZ reporter mice represent a powerful model to generally analyze the transcriptional control mechanisms involved in the induction of Vegfr2 expression during adult neovascularization...
  57. pmc Cardiomyocyte formation by skeletal muscle-derived multi-myogenic stem cells after transplantation into infarcted myocardium
    Tetsuro Tamaki
    Muscle Physiology and Cell Biology Unit, Tokai University School of Medicine, Isehara, Kanagawa, Japan
    PLoS ONE 3:e1789. 2008
    ..However, complete differentiation and/or trans-differentiation into cardiomyocytes have never occurred in these transplant studies, whereas functional contributions were reported...
  58. ncbi request reprint Role of progenitor endothelial cells in cardiovascular disease and upcoming therapies
    Atsuhiko Kawamoto
    Vascular Regeneration Research Group, Institute of Biomedical Research and Innovation, Kobe, Japan
    Catheter Cardiovasc Interv 70:477-84. 2007
    ....
  59. ncbi request reprint Endothelial progenitor cell culture and gene transfer
    Hideki Iwaguro
    Department of Regenerative Medicine, Tokai University School of Medicine, Kanagawa, Japan
    Methods Mol Med 112:239-47. 2005
    ..We discuss methodologies available for analysis of EPC cell therapy and gene-modified EPC therapy...
  60. ncbi request reprint Niche-dependent translineage commitment of endothelial progenitor cells, not cell fusion in general, into myocardial lineage cells
    Satoshi Murasawa
    Department of Regenerative Medicine, Institute of Biomedical Research and Innovation Stem Cell Translational Research, RIKEN Center for Developmental Biology, Kobe, Japan
    Arterioscler Thromb Vasc Biol 25:1388-94. 2005
    ..Our purpose was to investigate the mechanisms regulating EPC contribution to myocardial regeneration...
  61. ncbi request reprint Nitric oxide and angiogenesis in cardiovascular disease
    Toyoaki Murohara
    Department of Internal Medicine, Division of Cardiology, Nagoya University Graduate School of Medicine, Nagoya, Japan
    Antioxid Redox Signal 4:825-31. 2002
    ..In recent years, endothelium-derived NO has been shown to modulate angiogenesis in vitro and in vivo. In this review, we summarize recent progress in the field of the NO-mediated regulation of postnatal angiogenesis...
  62. ncbi request reprint Endothelial progenitor cells for vasculogenesis
    Satoshi Murasawa
    Department of Regenerative Medicine and Research, Kobe Institute of Biomedical Research and Innovation RIKEN, Center for Developmental Biology, Kobe Japan
    Physiology (Bethesda) 20:36-42. 2005
    ..EPCs might have an attractive potential therapeutic application for cardiovascular ischemic diseases as a novel cell-based strategy mainly via a vasculogenesis mechanism...
  63. ncbi request reprint Endothelial progenitor cells: past, state of the art, and future
    Yo Iwami
    Department of Regenerative Medicine Science, Tokai University School of Medicine, Bohseidai, Isehara, Kanagawa 259 1193, Japan
    J Cell Mol Med 8:488-97. 2004
    ..In this review, we attempt to discuss about biological features of EPCs and speculate on the clinical potential of EPCs for therapeutic neovascularization...
  64. ncbi request reprint Endothelial progenitor cell culture for vascular regeneration
    Masakazu Ishikawa
    Department of Regenerative Medicine, Institute of Biomedical Research and Innovation RIKEN Kobe Center for Developmental Biology, Kobe, Japan
    Stem Cells Dev 13:344-9. 2004
    ..In this article, we will discuss the sources that can generate EPCs and show the usefulness and potential of EPC culture using auto serum in the basic and clinical setting of neovascular formation...
  65. ncbi request reprint Newly emerging concepts in blood vessel growth: recent discovery of endothelial progenitor cells and their function in tissue regeneration
    Oren M Tepper
    Department of Surgery, Institute of Reconstructive Plastic Surgery, New York University Medical Center, New York, New York 10016, USA
    J Investig Med 51:353-9. 2003
    ..The following review article highlights the discovery of EPCs, their relationship to various clinical diseases, and their therapeutic potential for augmenting blood vessel formation...
  66. ncbi request reprint Bone marrow-derived endothelial progenitor cells for vascular regeneration
    Toshinori Murayama
    Division of Cardiovascular Research, St Elizabeth s Medical Center of Boston, Brighton, MA 02135 2997, USA
    Curr Opin Mol Ther 4:395-402. 2002
    ..This review highlights the discovery of BM-derived EPCs and their therapeutic potential for vascular regeneration...
  67. ncbi request reprint Proliferation, differentiation, and tube formation by endothelial progenitor cells in response to shear stress
    Kimiko Yamamoto
    Department of Biomedical Engineering, Graduate School of Medicine, University of Tokyo, 113 0033 Tokyo, Japan
    J Appl Physiol (1985) 95:2081-8. 2003
    ..These findings suggest that EPCs are sensitive to shear stress and that their vasculogenic activities may be modulated by shear stress...
  68. ncbi request reprint Functional recovery of damaged skeletal muscle through synchronized vasculogenesis, myogenesis, and neurogenesis by muscle-derived stem cells
    Tetsuro Tamaki
    Division of Basic Clinical Science, Department of Regenerative Medicine, Tokai University School of Medicine, Kanagawa 259 1193, Japan
    Circulation 112:2857-66. 2005
    ..In addition, the relationship between MDSCs and mesenchymal bone marrow cells is of interest...
  69. ncbi request reprint [Effects of statins on angiogenesis and vasculogenesis]
    Joan Llevadot
    Servicio de Cardiologia, Centro Cardiovascular Sant Jordi, Barcelona, Spain
    Rev Esp Cardiol 55:838-44. 2002
    ..Like vascular endothelial growth factor (VEGF), the statins promote angiogenesis and vasculogenesis. Therefore, Akt activation may explain some of the beneficial effects of the statins, including postnatal neovascularization...
  70. ncbi request reprint Estrogen-mediated endothelial progenitor cell biology and kinetics for physiological postnatal vasculogenesis
    Haruchika Masuda
    Department of Regenerative Medicine, Tokai University School of Medicine, Bohseidai, Isehara, Kanagawa 259 1193, Japan
    Circ Res 101:598-606. 2007
    ..These findings demonstrate that physiological postnatal vasculogenesis involves cyclic, E2-regulated bioactivity of BM-derived EPCs, predominantly through the ER alpha...
  71. ncbi request reprint Endothelial progenitor cells for postnatal vasculogenesis
    Masamichi Eguchi
    Department of Regenerative Medicine Science, Tokai University School of Medicine, Bohseidai, Isehara, Kanagawa, 259 1193, Japan
    Clin Exp Nephrol 11:18-25. 2007
    ..This review provides an update of the biology of EPCs, as well as highlighting the potential use of these cells for therapeutic regeneration...
  72. ncbi request reprint Hybrid cell-gene therapy for pulmonary hypertension based on phagocytosing action of endothelial progenitor cells
    Noritoshi Nagaya
    Department of Internal Medicine, National Cardiovascular Center, Osaka, Japan
    Circulation 108:889-95. 2003
    ..We investigated whether transplantation of vasodilator gene-transduced EPCs ameliorates monocrotaline (MCT)-induced pulmonary hypertension in rats...
  73. ncbi request reprint Gene modified cell transplantation for vascular regeneration
    Satoshi Murasawa
    Group of Vascular Regeneration, Institute of Biomedical Research and Innovation Stem Cell Translational Research Laboratory, RIKEN Kobe Institute Center for Developmental Biology, Kobe, Japan
    Curr Gene Ther 7:1-6. 2007
    ..Recently, several research reports have come out regarding gene modified cell transplantation. We will mainly focus on the background of EPC, and then gene modified EPC findings in this review...
  74. ncbi request reprint Nicotine enlivenment of blood flow recovery following endothelial progenitor cell transplantation into ischemic hindlimb
    Atsuhiko Sugimoto
    Department of Regenerative Medicine Science, Division of Cardiology, Tokai University School of Medicine, Bohseidai, Isehara, Kanagawa 259 1193, Japan
    Stem Cells Dev 16:649-56. 2007
    ..These findings suggest nicotine improves blood flow following EPC transplantation in patients with ischemic diseases...
  75. ncbi request reprint Endothelial E-selectin potentiates neovascularization via endothelial progenitor cell-dependent and -independent mechanisms
    Yasunobu Nishiwaki
    Department of Cardiovascular Medicine, Tokyo Medical and Dental University, School of Medicine, Tokyo, Japan
    Arterioscler Thromb Vasc Biol 27:512-8. 2007
    ....
  76. ncbi request reprint [Vascular endothelium regeneration therapy]
    Satoshi Murasawa
    Group for Vascular Regeneration Research, Institute of Biomedical Research and Innovation Stem Cell Translational Research Laboratory, Center for Developmental Biology, RIKEN Kobe Institute
    Nihon Rinsho 64:2135-41. 2006
    ..Ex vivo expansion or gene modification of EPCs could be the strategies for the next generation cell therapy to overcome these issues...
  77. ncbi request reprint Establishment of a functionally active collagen-binding vascular endothelial growth factor fusion protein in situ
    Tetsuya Ishikawa
    Department of Regenerative Medicine, Tokai University School of Medicine, Bohseidai, Isehara, Kanagawa 259 1193 Japan
    Arterioscler Thromb Vasc Biol 26:1998-2004. 2006
    ..We established FNCBD-VEGF121, consisting of the fibronectin collagen-binding domain (FNCBD) and vascular endothelial growth factor (VEGF) 121, and investigated its properties...
  78. ncbi request reprint Role of blood mononuclear cells in recanalization and vascularization of thrombi: past, present, and future
    Nicanor I Moldovan
    Davis Heart and Lung Research Institute, The Ohio State University, 473 12th Avenue, Room 305A, Columbus, OH 43210, USA
    Trends Cardiovasc Med 13:265-9. 2003
    ..These observations set the stage for therapeutic manipulation of organization and recanalization of thrombi, by increasing the MC/Mph and/or EPC content...
  79. ncbi request reprint Takayuki Asahara, M.D., PH.D. Interview by Vicki P. Glaser
    Takayuki Asahara
    J Hematother Stem Cell Res 11:579-82. 2002
  80. ncbi request reprint ACE inhibitor raps CD26/dipeptidylpeptidase IV knuckles for cytokine EPC mobilization
    Takayuki Asahara
    J Mol Cell Cardiol 41:8-10. 2006

Research Grants1

  1. POSTNATAL VASCULOGENESIS IN CARDIOVASCULAR DISEASE
    Takayuki Asahara; Fiscal Year: 2001
    ....