Ricardo Boland

..Of mechanistic relevance, an association between the VDR and TRP proteins with the participation of the scaffold protein INAD was shown...

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..Through these mechanisms, 1α,25(OH)(2)D(3) plays an important function in contractility and myogenesis...

..The myopathies associated to estrogen deficit states may be related to the mechanisms by which estrogen regulates apoptosis...

..These data raise the possibility that the antiapoptotic action of 17beta-estradiol in muscle cells may be related in part to a direct action of the hormone on mitochondria through ER beta...

..Impairment of 1alpha,25(OH)(2)D(3) activation of p38 MAPK upon ageing and abnormal hormone regulation of the c-fos oncoprotein synthesis may affect intestinal cell function...

..The results obtained in this work expand our knowledge on the mechanism of action of PTH in duodenal cells...

..ATP exerts diverse effects on various cell types via specific purinergic P2Y receptors. Intracellular signaling cascades are the main routes of communication between P2Y receptors and regulatory targets in the cell...

..Altogether, these data indicate that 17β-estradiol activates ERK2 through ERα and p38 MAPK in an ERα/β-independent manner and that PKC and Src proteins are key upstream components on MAPKs activation in C2C12 skeletal muscle cells...

..These findings suggest that ATP modulation of P2Y(2/4) receptors increases MCF-7 cell proliferation by activation of the PI3K/Akt signaling pathway through PLC/IP(3)/Ca(2+), PKC and Src...

..Our results suggest that 1alpha,25(OH)(2)D(3) activates p38 MAPK, involving Ca(2+), c-Src and PKA as upstream regulators, and that p38 MAPK has a central role in hormone-induction of the oncoprotein c-Fos in rat intestinal cells...

..Altogether, these results show that E(2) exerts a survival action in skeletal muscle cells involving ERK and p38 MAPK activation...

..In previous work, we have demonstrated that extracellular adenosine 5'-triphosphate (ATP) acts on intestinal Caco-2 cell P2Y receptors promoting a rapid increase in the phosphorylation of ERK1/2, p46 JNK and p38 MAP kinases (MAPKs)...

..These results suggest that MAPK signalling pathways play an important role in regulating early gene expression through CREB and Elk-1 activation in skeletal muscle cells...

..These data suggest that 1α,25(OH)(2)D(3) acts in skeletal muscle upstream on MAPK cascades sequentially activating PKC, PTPα and Src...

..8 cells in a way dependent on PI-PLC/IP(3)/intracellular Ca(2+) release and Ca(2+) influx through stress activated and L-type voltage-dependent calcium channels and involves PKC and Src kinases...

..These results suggest that stimulation of P2Y(2) and P2Y(4) receptors by ATP modulates transcription factors through PKC/MAPKs and PKC/Src pathways in MCF-7 cells...

..These results provide new evidence on the existence of a BP target in osteoblastic cells, presumably a PTP, which may be involved in the stimulatory action of BPs on osteoblast proliferation...

..PKC seems to regulate Akt activation through Src and the Ca²⁺ influx/CaM pathway...

..Altogether, these data suggest that caveolae is involved upstream in c-Src-MAPKs activation by 1alpha,25(OH)2D3 and that VDR and cav-1 participate in the rapid signaling elicited by the hormone...

..These results indicate that E2 exerts antiapoptotic effects in skeletal muscle cells which are mediated by ER-beta and ER-alpha and involve the PI3K/Akt pathway...

..The non-classical distribution of native pools of ER alpha in skeletal muscle cells suggests an alternative mode of ER localization/function...

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..Studies with ERK1/2 specific inhibitor PD98059, p38 MAPK inhibitor SB203580, or JNK inhibitor SP600125 showed that FBS regulates Caco-2 cell proliferation via the three MAPK pathways...

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..This review summarizes the molecular basis of the modulation of programmed cell death by E2 and T in several tissues...

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..Then, this study suggests that HSP27 plays a new role in the antiapoptotic action triggered by 17beta-estradiol by modulating caspase-3 activity and stabilizing ERbeta in skeletal muscle cells...

..Altogether, these results suggest that down-regulation of the NFκB pathway is part of the mechanism involved in the antiproliferative effects of 1α,25(OH)(2)D(3) on endothelial cells transformed by vGPCR...

..The data presented allow us to begin to elucidate the mechanism by which the hormone prevents apoptosis in skeletal muscle...

..We anticipate TRPC3 as a candidate for mediating store-operated non-selective cation entry into osteoblasts...

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..The relative contribution of ERK-1/2, p38, and JNK-1/2 and their interrelationships in hormonal regulation of muscle cell proliferation and differentiation remain to be established...

..Copyright Wiley-Liss, Inc...

..The non-classical distribution of native pools of AR in skeletal muscle cells suggests an alternative mode of AR localization/function...

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..Also, the signal transduction pathways regulated by these complexes and their new functional properties are discussed...

..Taken as a whole, the results indicate that ER-beta could be expressed as functional estrogen binding proteins among a dominant population of ER-alpha sites in the cell lines under study...

..Our results suggest a role of E(2) in the regulation of apoptosis with a clear action at the mitochondrial level in C2C12 skeletal myoblast cells...

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..In SKBR3 cells, the relative contribution to CCE of both routes was opposite to that in non-tumorigenic cells...