A G Schijman

Summary

Country: Argentina

Publications

  1. pmc International study to evaluate PCR methods for detection of Trypanosoma cruzi DNA in blood samples from Chagas disease patients
    Alejandro G Schijman
    Laboratorio de Biología Molecular de Enfermedad de Chagas, Instituto de Investigaciones en Ingeniería Genética y Biología Molecular INGEBI CONICET, Buenos Aires, Argentina
    PLoS Negl Trop Dis 5:e931. 2011
  2. ncbi request reprint Aetiological treatment of congenital Chagas' disease diagnosed and monitored by the polymerase chain reaction
    Alejandro G Schijman
    Laboratorio de Biologia Molecular de la Enfermedad de Chagas, Instituto de Ingeniería Genética y Biología Molecular INGEBI, Buenos Aires, Argentina
    J Antimicrob Chemother 52:441-9. 2003
  3. ncbi request reprint High variability of Colombian Trypanosoma cruzi lineage I stocks as revealed by low-stringency single primer-PCR minicircle signatures
    Alexander Salazar
    Grupo de Chagas, Corporación de Patologías Tropicales, Instituto de Biologia, Universidad de Antioquia, Medellin, Colombia
    Acta Trop 100:110-8. 2006
  4. pmc Accurate real-time PCR strategy for monitoring bloodstream parasitic loads in chagas disease patients
    Tomas Duffy
    Laboratorio de Biologia Molecular de la Enfermedad de Chagas, Instituto de Investigaciones en Ingeniería Genética y Biología Molecular INGEBI CONICET, Buenos Aires, Argentina
    PLoS Negl Trop Dis 3:e419. 2009
  5. pmc Analytical performance of a multiplex Real-Time PCR assay using TaqMan probes for quantification of Trypanosoma cruzi satellite DNA in blood samples
    Tomas Duffy
    Instituto de Investigaciones en Ingeniería Genética y Biología Molecular Dr Hector N Torres INGEBI CONICET, Buenos Aires, Argentina
    PLoS Negl Trop Dis 7:e2000. 2013
  6. pmc Evolution of naturally occurring 5'non-coding region variants of Hepatitis C virus in human populations of the South American region
    Gonzalo Moratorio
    Laboratorio de Virologia Molecular, Centro de Investigaciones Nucleares, Facultad de Ciencias, Igua, Montevideo, Uruguay
    Virol J 4:79. 2007
  7. ncbi request reprint Differential detection of Blastocrithidia triatomae and Trypanosoma cruzi by amplification of 24salpha ribosomal RNA genes in faeces of sylvatic triatomine species from rural northwestern Argentina
    A G Schijman
    Laboratorio de Biologia Molecular de la Enfermedad de Chagas, Instituto de Ingeniería Genética y Biología Molecular INGEBI CONICET, Buenos Aires, Argentina
    Acta Trop 99:50-4. 2006
  8. ncbi request reprint Prospective evaluation of in-house polymerase chain reaction for diagnosis of mycobacterial diseases in patients with HIV infection and lung infiltrates
    A G Schijman
    Laboratorios BioCiencia Grupo CentraLab, Buenos Aires, Argentina
    Int J Tuberc Lung Dis 8:106-13. 2004
  9. pmc Detection of Trypanosoma cruzi infection in naturally infected dogs and cats using serological, parasitological and molecular methods
    G F Enriquez
    Laboratorio de Eco Epidemiología, Departamento de Ecologia, Genetica y Evolucion, Universidad de Buenos Aires, Buenos Aires, Argentina
    Acta Trop 126:211-7. 2013
  10. pmc Discrete typing units of Trypanosoma cruzi identified in rural dogs and cats in the humid Argentinean Chaco
    G F Enriquez
    Laboratorio de Eco Epidemiología, Departamento de Ecologia, Genetica y Evolucion, Universidad de Buenos Aires, Buenos Aires, Argentina
    Parasitology 140:303-8. 2013

Collaborators

Detail Information

Publications22

  1. pmc International study to evaluate PCR methods for detection of Trypanosoma cruzi DNA in blood samples from Chagas disease patients
    Alejandro G Schijman
    Laboratorio de Biología Molecular de Enfermedad de Chagas, Instituto de Investigaciones en Ingeniería Genética y Biología Molecular INGEBI CONICET, Buenos Aires, Argentina
    PLoS Negl Trop Dis 5:e931. 2011
    ..The purpose of this study was to evaluate the performance of PCR methods in detection of Trypanosoma cruzi DNA by an external quality evaluation...
  2. ncbi request reprint Aetiological treatment of congenital Chagas' disease diagnosed and monitored by the polymerase chain reaction
    Alejandro G Schijman
    Laboratorio de Biologia Molecular de la Enfermedad de Chagas, Instituto de Ingeniería Genética y Biología Molecular INGEBI, Buenos Aires, Argentina
    J Antimicrob Chemother 52:441-9. 2003
    ..This prospective study focused on the evaluation of anti-parasitic therapy in congenital Chagas' disease, diagnosed and monitored by PCR and conventional diagnosis...
  3. ncbi request reprint High variability of Colombian Trypanosoma cruzi lineage I stocks as revealed by low-stringency single primer-PCR minicircle signatures
    Alexander Salazar
    Grupo de Chagas, Corporación de Patologías Tropicales, Instituto de Biologia, Universidad de Antioquia, Medellin, Colombia
    Acta Trop 100:110-8. 2006
    ..cruzi I stocks from a same region suggest that they may have a multiclonal character. Our results show that LSSP-PCR is a fast, valuable technique for characterization of intra-lineage polymorphism among T. cruzi stocks...
  4. pmc Accurate real-time PCR strategy for monitoring bloodstream parasitic loads in chagas disease patients
    Tomas Duffy
    Laboratorio de Biologia Molecular de la Enfermedad de Chagas, Instituto de Investigaciones en Ingeniería Genética y Biología Molecular INGEBI CONICET, Buenos Aires, Argentina
    PLoS Negl Trop Dis 3:e419. 2009
    ..This report describes a real-time PCR (Q-PCR) strategy to quantify Trypanosoma cruzi (T. cruzi) DNA in peripheral blood samples from Chagas disease patients targeted to conserved motifs within the repetitive satellite sequence...
  5. pmc Analytical performance of a multiplex Real-Time PCR assay using TaqMan probes for quantification of Trypanosoma cruzi satellite DNA in blood samples
    Tomas Duffy
    Instituto de Investigaciones en Ingeniería Genética y Biología Molecular Dr Hector N Torres INGEBI CONICET, Buenos Aires, Argentina
    PLoS Negl Trop Dis 7:e2000. 2013
    ....
  6. pmc Evolution of naturally occurring 5'non-coding region variants of Hepatitis C virus in human populations of the South American region
    Gonzalo Moratorio
    Laboratorio de Virologia Molecular, Centro de Investigaciones Nucleares, Facultad de Ciencias, Igua, Montevideo, Uruguay
    Virol J 4:79. 2007
    ....
  7. ncbi request reprint Differential detection of Blastocrithidia triatomae and Trypanosoma cruzi by amplification of 24salpha ribosomal RNA genes in faeces of sylvatic triatomine species from rural northwestern Argentina
    A G Schijman
    Laboratorio de Biologia Molecular de la Enfermedad de Chagas, Instituto de Ingeniería Genética y Biología Molecular INGEBI CONICET, Buenos Aires, Argentina
    Acta Trop 99:50-4. 2006
    ..This PCR procedure provides a rapid sensitive tool for differential diagnosis of morphologically similar trypanosomatids in field surveys of Chagas disease vectors and laboratory-reared triatomines used for xenodiagnosis...
  8. ncbi request reprint Prospective evaluation of in-house polymerase chain reaction for diagnosis of mycobacterial diseases in patients with HIV infection and lung infiltrates
    A G Schijman
    Laboratorios BioCiencia Grupo CentraLab, Buenos Aires, Argentina
    Int J Tuberc Lung Dis 8:106-13. 2004
    ..The inadequate sensitivity of Ziehl-Neelsen staining and its inability to distinguish atypical mycobacteria delays accurate diagnosis...
  9. pmc Detection of Trypanosoma cruzi infection in naturally infected dogs and cats using serological, parasitological and molecular methods
    G F Enriquez
    Laboratorio de Eco Epidemiología, Departamento de Ecologia, Genetica y Evolucion, Universidad de Buenos Aires, Buenos Aires, Argentina
    Acta Trop 126:211-7. 2013
    ..82). The high sensitivity of kDNA-PCR to detect T. cruzi infections in naturally infected dogs and cats supports its application as a diagnostic tool complementary to serology and may replace the use of xenodiagnosis or hemoculture...
  10. pmc Discrete typing units of Trypanosoma cruzi identified in rural dogs and cats in the humid Argentinean Chaco
    G F Enriquez
    Laboratorio de Eco Epidemiología, Departamento de Ecologia, Genetica y Evolucion, Universidad de Buenos Aires, Buenos Aires, Argentina
    Parasitology 140:303-8. 2013
    ..Our study reinforces the importance of dogs and cats as domestic reservoir hosts and sources of various DTUs infecting humans, and suggests a link between dogs and the sylvatic transmission cycle of TcIII...
  11. pmc Direct molecular identification of Trypanosoma cruzi discrete typing units in domestic and peridomestic Triatoma infestans and Triatoma sordida from the Argentine Chaco
    L Maffey
    Laboratorio de Eco Epidemiología, Departamento de Ecologia, Genetica y Evolucion, Universidad de Buenos Aires, Buenos Aires, Argentina
    Parasitology 139:1570-9. 2012
    ..T. sordida is a probable sylvatic vector of TcI linked to D. albiventris, and could represent a secondary vector of TcVI and TcV in the domestic/peridomestic cycle...
  12. doi request reprint Trypanosoma cruzi discrete typing units in Chagas disease patients from endemic and non-endemic regions of Argentina
    C I Cura
    Laboratorio de Biologia Molecular de la Enfermedad de Chagas, Instituto de Ingeniería Genética y Biología Molecular, Vuelta de Obligado 2490, 2do piso, 1428, Ciudad de Buenos Aires, Argentina
    Parasitology 139:516-21. 2012
    ..In conclusion, our findings provide evidence of the complexity of the dynamics of T. cruzi diversity in the natural history of human Chagas disease and allege the pathogenic role of DTUs I, II, V and VI in the studied population...
  13. doi request reprint Real-time PCR strategy for rapid discrimination among main lymnaeid species from Argentina
    T Duffy
    Laboratorio de Biología Molecular de Enfermedad de Chagas, Instituto de Ingeniería Genética y Biología Molecular INGEBI CONICET, Vuelta de Obligado 2490, 1427 Buenos Aires, Argentina
    Acta Trop 109:1-4. 2009
    ..This molecular approach constitutes a key laboratory tool complementing ecological studies that ultimately will promote more efficient control strategies...
  14. pmc The sylvatic transmission cycle of Trypanosoma cruzi in a rural area in the humid Chaco of Argentina
    J A Alvarado-Otegui
    Laboratorio de Eco Epidemiología, Departamento de Ecologia, Genetica y Evolucion, Universidad de Buenos Aires, Argentina
    Acta Trop 124:79-86. 2012
    ..cruzi by microscopical examination or kDNA-PCR. Our results indicate that two independent sylvatic transmission cycles of T. cruzi occur in the humid Chaco. The putative vectors of both cycles need to be identified conclusively...
  15. ncbi request reprint Direct molecular profiling of minicircle signatures and lineages of Trypanosoma cruzi bloodstream populations causing congenital Chagas disease
    Juan M Burgos
    Laboratorio de Biología Molecular de la Enfermedad de Chagas LaBMECh, Instituto de Investigaciones en Ingeniería Genética y Biología Molecular INGEBI CONICET, Buenos Aires, Argentina
    Int J Parasitol 37:1319-27. 2007
    ..Moreover, allelic size polymorphism analysis of microsatellite loci from populations transmitted to twins showed that all clones from the maternal polyclonal population were equally infective to both siblings...
  16. ncbi request reprint Molecular identification of Trypanosoma cruzi I tropism for central nervous system in Chagas reactivation due to AIDS
    Juan M Burgos
    Laboratorio de Biologia Molecular de la Enfermedad de Chagas, Instituto de Investigaciones en Ingeniería Genética y Biología Molecular INGEBI CONICET, Buenos Aires, Argentina
    Am J Trop Med Hyg 78:294-7. 2008
    ..cruzi I population. Our findings do not corroborate the original assumption of innocuity for T. cruzi I in the southern cone of the Americas and highlight lineage I tropism for central nervous system causing lethal Chagas reactivation...
  17. doi request reprint Molecular diagnosis and treatment monitoring of congenital transmission of Trypanosoma cruzi to twins of a triplet delivery
    Juan M Burgos
    Laboratorio de Biologia Molecular de la Enfermedad de Chagas, Instituto de Investigaciones en Ingeniería Genética y Biología Molecular INGEBI CONICET, Buenos Aires C1428ADL, Argentina
    Diagn Microbiol Infect Dis 65:58-61. 2009
    ..The neonates' parasitologic response to benznidazole was monitored. Molecular strategies allowed genotyping lineage IId and identical minicircle signatures in both triplets, showing they harbored the same maternal parasite populations...
  18. ncbi request reprint Trypanosoma cruzi DNA in cardiac lesions of Argentinean patients with end-stage chronic chagas heart disease
    Alejandro G Schijman
    Laboratorio de Biologia Molecular de la Enfermedad de Chagas, INGEBI, Consejo Nacional de Ciencia y Tecnologia, Department of Fisiology, Celular and Molecular Biology, F CEyN, University of Buenos Aires, Buenos Aires, Argentina
    Am J Trop Med Hyg 70:210-20. 2004
    ..001); and 4) 45% and 90% of the specimens from cChHD patients without or with heart failure, respectively (P < 0.01). These findings stress the role of the parasite in pathogenesis and disease progression of cChHD...
  19. ncbi request reprint Molecular diagnosis and typing of Trypanosoma cruzi populations and lineages in cerebral Chagas disease in a patient with AIDS
    Juan M Burgos
    Laboratorio de Biologia Molecular de la Enfermedad de Chagas, Instituto de Investigaciones en Ingeniería Genética y Biología Molecular INGEBI CONICET, Buenos Aires, Argentina
    Am J Trop Med Hyg 73:1016-8. 2005
    ..This is the first case report demonstrating a differential distribution of natural parasite populations and sublineages in Chagas disease reactivation, showing the proliferation of cerebral variants not detectable in peripheral blood...
  20. doi request reprint Early molecular diagnosis of acute Chagas disease after transplantation with organs from Trypanosoma cruzi-infected donors
    C I Cura
    Laboratorio de Biologia Molecular de la Enfermedad de Chagas, Instituto de Investigaciones en Ingeniería Genética y Biología Molecular Dr Hector N Torres INGEBI CONICET, Buenos Aires, Argentina
    Am J Transplant 13:3253-61. 2013
    ..The five cases were infected by DTU TcV. This report reveals the relevance of systematic monitoring of TX recipients using PCR strategies in order to provide an early diagnosis allowing timely anti-trypanosomal treatment...
  21. pmc Impairment of infectivity and immunoprotective effect of a LYT1 null mutant of Trypanosoma cruzi
    M Paola Zago
    Instituto de Patología Experimental CONICET, Universidad Nacional de Salta, Calle Buenos Aires 177, 4400 Salta, Argentina
    Infect Immun 76:443-51. 2008
    ..A strong protection against a virulent challenge in both L16- and WT-infected mice was observed; however, the immunizing infection by the genetically modified parasite was highly attenuated...
  22. ncbi request reprint Amiodarone has intrinsic anti-Trypanosoma cruzi activity and acts synergistically with posaconazole
    Gustavo Benaim
    Laboratorio Química Biológica and Laboratorio de Permeabilidad Iónica, Instituto Venezolano de Investigaciones Cientificas, Apartado 21927, Caracas 1020A, Venezuela
    J Med Chem 49:892-9. 2006
    ..cruzi and open up the possibility of novel, combination therapy approaches to the treatment of Chagas' disease using currently approved drugs...