Thirunavukkarasu Annamalai

Summary

Publications

  1. pmc Analysis of DNA relaxation and cleavage activities of recombinant Mycobacterium tuberculosis DNA topoisomerase I from a new expression and purification protocol
    Thirunavukkarasu Annamalai
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York, USA
    BMC Biochem 10:18. 2009
  2. pmc The strictly conserved Arg-321 residue in the active site of Escherichia coli topoisomerase I plays a critical role in DNA rejoining
    Gagandeep Narula
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York 10595, USA
    J Biol Chem 286:18673-80. 2011
  3. pmc Asp-to-Asn substitution at the first position of the DxD TOPRIM motif of recombinant bacterial topoisomerase I is extremely lethal to E. coli
    Bokun Cheng
    Department of Biochemistry and Molecular Biology, Basic Science Building, New York Medical College, Valhalla, NY 10595, USA
    J Mol Biol 385:558-67. 2009
  4. pmc Identification of anziaic acid, a lichen depside from Hypotrachyna sp., as a new topoisomerase poison inhibitor
    Bokun Cheng
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York, United States of America
    PLoS ONE 8:e60770. 2013
  5. pmc Hydroxyl radicals are involved in cell killing by the bacterial topoisomerase I cleavage complex
    I Fen Liu
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York 10595, USA
    J Bacteriol 191:5315-9. 2009

Collaborators

Detail Information

Publications5

  1. pmc Analysis of DNA relaxation and cleavage activities of recombinant Mycobacterium tuberculosis DNA topoisomerase I from a new expression and purification protocol
    Thirunavukkarasu Annamalai
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York, USA
    BMC Biochem 10:18. 2009
    ..It shares a common transesterification domain with other type IA DNA topoisomerases. There is, however, no homology between the C-terminal DNA binding domains of Escherichia coli and M. tuberculosis DNA topoisomerase I proteins...
  2. pmc The strictly conserved Arg-321 residue in the active site of Escherichia coli topoisomerase I plays a critical role in DNA rejoining
    Gagandeep Narula
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York 10595, USA
    J Biol Chem 286:18673-80. 2011
    ..Small molecules that can interfere or distort the enzyme-DNA interactions required for DNA rejoining by bacterial type IA topoisomerases could be developed into novel antibacterial drugs...
  3. pmc Asp-to-Asn substitution at the first position of the DxD TOPRIM motif of recombinant bacterial topoisomerase I is extremely lethal to E. coli
    Bokun Cheng
    Department of Biochemistry and Molecular Biology, Basic Science Building, New York Medical College, Valhalla, NY 10595, USA
    J Mol Biol 385:558-67. 2009
    ..The extreme sensitivity of the first TOPRIM position suggested that this might be a useful site for binding of small molecules that could act as topoisomerase poisons...
  4. pmc Identification of anziaic acid, a lichen depside from Hypotrachyna sp., as a new topoisomerase poison inhibitor
    Bokun Cheng
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York, United States of America
    PLoS ONE 8:e60770. 2013
    ..This is the first report of a depside with activity as a topoisomerase poison inhibitor and demonstrates the potential of this class of natural products as a source for new antibacterial and anticancer compounds...
  5. pmc Hydroxyl radicals are involved in cell killing by the bacterial topoisomerase I cleavage complex
    I Fen Liu
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York 10595, USA
    J Bacteriol 191:5315-9. 2009
    ..The presence of the Fe(2+) chelator 2,2'-dipyridyl and an iscS mutation affecting Fe-S cluster formation protect against topoisomerase I cleavage complex-mediated cell killing...