Thirunavukkarasu Annamalai

Summary

Publications

  1. pmc Analysis of DNA relaxation and cleavage activities of recombinant Mycobacterium tuberculosis DNA topoisomerase I from a new expression and purification protocol
    Thirunavukkarasu Annamalai
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York, USA
    BMC Biochem 10:18. 2009
  2. pmc The strictly conserved Arg-321 residue in the active site of Escherichia coli topoisomerase I plays a critical role in DNA rejoining
    Gagandeep Narula
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York 10595, USA
    J Biol Chem 286:18673-80. 2011
  3. pmc Asp-to-Asn substitution at the first position of the DxD TOPRIM motif of recombinant bacterial topoisomerase I is extremely lethal to E. coli
    Bokun Cheng
    Department of Biochemistry and Molecular Biology, Basic Science Building, New York Medical College, Valhalla, NY 10595, USA
    J Mol Biol 385:558-67. 2009
  4. pmc Identification of anziaic acid, a lichen depside from Hypotrachyna sp., as a new topoisomerase poison inhibitor
    Bokun Cheng
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York, United States of America
    PLoS ONE 8:e60770. 2013
  5. pmc Inhibition of Zn(II) Binding Type IA Topoisomerases by Organomercury Compounds and Hg(II)
    Bokun Cheng
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York, United States of America
    PLoS ONE 10:e0120022. 2015
  6. pmc Hydroxyl radicals are involved in cell killing by the bacterial topoisomerase I cleavage complex
    I Fen Liu
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York 10595, USA
    J Bacteriol 191:5315-9. 2009

Collaborators

Detail Information

Publications6

  1. pmc Analysis of DNA relaxation and cleavage activities of recombinant Mycobacterium tuberculosis DNA topoisomerase I from a new expression and purification protocol
    Thirunavukkarasu Annamalai
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York, USA
    BMC Biochem 10:18. 2009
    ..It shares a common transesterification domain with other type IA DNA topoisomerases. There is, however, no homology between the C-terminal DNA binding domains of Escherichia coli and M. tuberculosis DNA topoisomerase I proteins...
  2. pmc The strictly conserved Arg-321 residue in the active site of Escherichia coli topoisomerase I plays a critical role in DNA rejoining
    Gagandeep Narula
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York 10595, USA
    J Biol Chem 286:18673-80. 2011
    ..Small molecules that can interfere or distort the enzyme-DNA interactions required for DNA rejoining by bacterial type IA topoisomerases could be developed into novel antibacterial drugs...
  3. pmc Asp-to-Asn substitution at the first position of the DxD TOPRIM motif of recombinant bacterial topoisomerase I is extremely lethal to E. coli
    Bokun Cheng
    Department of Biochemistry and Molecular Biology, Basic Science Building, New York Medical College, Valhalla, NY 10595, USA
    J Mol Biol 385:558-67. 2009
    ..The extreme sensitivity of the first TOPRIM position suggested that this might be a useful site for binding of small molecules that could act as topoisomerase poisons...
  4. pmc Identification of anziaic acid, a lichen depside from Hypotrachyna sp., as a new topoisomerase poison inhibitor
    Bokun Cheng
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York, United States of America
    PLoS ONE 8:e60770. 2013
    ..This is the first report of a depside with activity as a topoisomerase poison inhibitor and demonstrates the potential of this class of natural products as a source for new antibacterial and anticancer compounds...
  5. pmc Inhibition of Zn(II) Binding Type IA Topoisomerases by Organomercury Compounds and Hg(II)
    Bokun Cheng
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York, United States of America
    PLoS ONE 10:e0120022. 2015
    ..The Zn(II) binding domains found in human Top3α and Top3β may be potential targets of toxic metals and organometallic complexes, with potential consequence on genomic stability and development. ..
  6. pmc Hydroxyl radicals are involved in cell killing by the bacterial topoisomerase I cleavage complex
    I Fen Liu
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York 10595, USA
    J Bacteriol 191:5315-9. 2009
    ..The presence of the Fe(2+) chelator 2,2'-dipyridyl and an iscS mutation affecting Fe-S cluster formation protect against topoisomerase I cleavage complex-mediated cell killing...