Peroxiredoxin 6 translocates to the plasma membrane during neutrophil activation and is required for optimal NADPH oxidase activityDaniel R Ambruso
Bonfils Blood Center, Denver, CO 80230, USA
Biochim Biophys Acta 1823:306-15. 2012
..Prdx6 supports retention of the active oxidase complex in stimulated plasma membrane, and results with mutant proteins imply that Prdx6 serves an additional biochemical or structural role in supporting optimal NADPH oxidase activity...
- Lack of antibody formation to platelet neoantigens after transfusion of riboflavin and ultraviolet light-treated platelet concentrates
Daniel R Ambruso
Bonfils Blood Center, Denver, Colorado, USA
Transfusion 49:2631-6. 2009
..This study evaluated serum of patients who received PRT-treated platelet (PLT) concentrates over a time period of 28 days for the appearance of antibodies to neoantigens on PLTs...
Formyl-Met-Leu-Phe induces calcium-dependent tyrosine phosphorylation of Rel-1 in neutrophilsMarguerite R Kelher
Department of Surgery, University of Colorado Health Science Center School of Medicine, Denver, CO 80230, USA
Cell Calcium 34:445-55. 2003
..Thus, fMLP concentrations that prime or activate the oxidase cause a rapid Ca2+-dependent tyrosine phosphorylation of Rel-1 involving p38 MAPK activation...
- NADPH oxidase activity of neutrophil specific granules: requirements for cytosolic components and evidence of assembly during cell activation
Daniel R Ambruso
Department of Pediatrics, The Bonfils Blood Center, The University of Colorado School of Medicine, Denver, CO, USA
Mol Genet Metab 81:313-21. 2004
..These studies establish a critical role for specific granules as a site for assembly and activation of the oxidase enzyme system and an important constituent for the microbicidal activity of the neutrophil...
- CP-64131, an aminobenzazepine with cytokine-like properties, stimulates human neutrophil functions through the p38-MAPK pathway
Marsha S Anderson
Bonfils Blood Center, 717 Yosemite Street, Denver, CO 80230, USA
J Leukoc Biol 76:477-83. 2004
..CP has the ability to activate specific MAPK pathways in different cell types and should prove to be an effective agonist in combination with inhibitors to study biological responses regulated by MAPKs...
- Therapeutic options for transfusion related acute lung injury; the potential of the G2A receptor
Michael A Ellison
Belle Bonfils Memorial Blood Center, Denver, CO 80230, USA
Curr Pharm Des 18:3255-9. 2012
..Additionally, characterization of the process by which lyso-PCs are generated in stored blood products could allow development of inhibitors and additive solutions to block their formation in the first place...
- Generation of neutrophil priming activity by cell-containing blood components treated with pathogen reduction technology and stored in platelet additive solutions
Daniel R Ambruso
Bonfils Blood Center and the Center for Cancer and Blood Disorders, The Children s Hospital, Denver, Colorado, USA
Transfusion 51:1220-7. 2011
..We evaluated the priming activity of pathogen reduction technology (PRT)-treated PCs stored in plasma or platelet additive solution (PAS) and PRT-treated RBCs...
- Oxidase activity in cord blood neutrophils: a balance between increased membrane associated cytochrome b558 and deficient cytosolic components
Urmish H Chudgar
Department of Pediatrics, University of Colorado School of Medicine, 4200 East Ninth Avenue B128, Denver, CO 80262, USA
Pediatr Blood Cancer 45:311-7. 2005
....
- Phox activity of differentiated PLB-985 cells is enhanced, in an agonist specific manner, by the PLA2 activity of Prdx6-PLA2
Michael A Ellison
Bonfils Blood Center, Denver, CO 80230, USA
Eur J Immunol 42:1609-17. 2012
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- A 29-kDa protein associated with p67phox expresses both peroxiredoxin and phospholipase A2 activity and enhances superoxide anion production by a cell-free system of NADPH oxidase activity
Patrick J Leavey
Bonfils Blood Center, Denver, Colorado 80230, USA
J Biol Chem 277:45181-7. 2002
..Thus, p29 is a novel protein that associates with p67 and has peroxiredoxin activity. This protein has a potential role in protecting the NADPH oxidase by inactivating H(2)O(2) or altering signaling pathways affected by H(2)O(2)...
- Lysophosphatidylcholines prime the NADPH oxidase and stimulate multiple neutrophil functions through changes in cytosolic calcium
Christopher C Silliman
Bonfils Blood Center, Denver, Colorado 80230, USA
J Leukoc Biol 73:511-24. 2003
..In conclusion, lyso-PCs affect multiple PMN functions in a Ca(2)(+)-dependent manner that involves the activation of a pertussis toxin-sensitive G-protein...
- Transfusion-related acute lung injury
Christopher C Silliman
Bonfils Blood Center, 717 Yosemite St, Denver, CO 80230, USA
Blood 105:2266-73. 2005
..Prevention, treatment, and a proposed definition of TRALI, especially in the context of ALI, are also examined...
- Long term G-CSF-induced remission of ulcerative colitis-like inflammatory bowel disease in a patient with glycogen storage disease Ib and evaluation of associated neutrophil function
Abdulrahman Alsultan
Department of Pediatrics and Pathology, University of Colorado Denver, Aurora, Colorado, USA
Pediatr Blood Cancer 55:1410-3. 2010
..Ulcerative colitis-like IBD may also be seen in GSD Ib and is responsive to G-CSF therapy. Neutrophil dysfunction is variable among patients with GSD Ib...
- Rac2D57N, a dominant inhibitory Rac2 mutant that inhibits p38 kinase signaling and prevents surface ruffling in bone-marrow-derived macrophages
Amy N Abell
Department of Pharmacology, University of Colorado Health Sciences Center, 4200 East Ninth Ave, Denver, CO 80262, USA
J Cell Sci 117:243-55. 2004
..Enhanced binding of Rac2D57N to its upstream regulators would inhibit Rac-dependent effects on actin cytoskeletal dynamics and p38 kinase signaling...
- Transfusion-related acute lung injury: epidemiology and a prospective analysis of etiologic factors
Christopher C Silliman
Bonfils Blood Center and the Department of Pediatrics, University of Colorado School of Medicine, Denver, USA
Blood 101:454-62. 2003
..TRALI, like the acute respiratory distress syndrome, may be a 2-event phenomenon with both recipient predisposition and factors in the stored units playing major roles...
- In vivo treatment with granulocyte colony-stimulating factor does not delay apoptosis in human neutrophils by increasing the expression of the vacuolar proton ATPase
Julie Zimbelman
Bonfils Blood Center, Department of Pediatrics, University of Colorado School of Medicine, Denver 80230, USA
J Investig Med 50:33-7. 2002
..CONCLUSION: In vivo G-CSF does not increase the amount of v-ATPase in neutrophils. Although G-CSF in vivo delays apoptosis, the mechanism(s) by which this occurs is not known...
