Daniel R Ambruso

Summary

Publications

  1. doi Peroxiredoxin 6 translocates to the plasma membrane during neutrophil activation and is required for optimal NADPH oxidase activity
    Daniel R Ambruso
    Bonfils Blood Center, Denver, CO 80230, USA
    Biochim Biophys Acta 1823:306-15. 2012
  2. doi Lack of antibody formation to platelet neoantigens after transfusion of riboflavin and ultraviolet light-treated platelet concentrates
    Daniel R Ambruso
    Bonfils Blood Center, Denver, Colorado, USA
    Transfusion 49:2631-6. 2009
  3. ncbi Formyl-Met-Leu-Phe induces calcium-dependent tyrosine phosphorylation of Rel-1 in neutrophils
    Marguerite R Kelher
    Department of Surgery, University of Colorado Health Science Center School of Medicine, Denver, CO 80230, USA
    Cell Calcium 34:445-55. 2003
  4. ncbi NADPH oxidase activity of neutrophil specific granules: requirements for cytosolic components and evidence of assembly during cell activation
    Daniel R Ambruso
    Department of Pediatrics, The Bonfils Blood Center, The University of Colorado School of Medicine, Denver, CO, USA
    Mol Genet Metab 81:313-21. 2004
  5. ncbi CP-64131, an aminobenzazepine with cytokine-like properties, stimulates human neutrophil functions through the p38-MAPK pathway
    Marsha S Anderson
    Bonfils Blood Center, 717 Yosemite Street, Denver, CO 80230, USA
    J Leukoc Biol 76:477-83. 2004
  6. ncbi A 29-kDa protein associated with p67phox expresses both peroxiredoxin and phospholipase A2 activity and enhances superoxide anion production by a cell-free system of NADPH oxidase activity
    Patrick J Leavey
    Bonfils Blood Center, Denver, Colorado 80230, USA
    J Biol Chem 277:45181-7. 2002
  7. pmc Therapeutic options for transfusion related acute lung injury; the potential of the G2A receptor
    Michael A Ellison
    Belle Bonfils Memorial Blood Center, Denver, CO 80230, USA
    Curr Pharm Des 18:3255-9. 2012
  8. ncbi Oxidase activity in cord blood neutrophils: a balance between increased membrane associated cytochrome b558 and deficient cytosolic components
    Urmish H Chudgar
    Department of Pediatrics, University of Colorado School of Medicine, 4200 East Ninth Avenue B128, Denver, CO 80262, USA
    Pediatr Blood Cancer 45:311-7. 2005
  9. doi Generation of neutrophil priming activity by cell-containing blood components treated with pathogen reduction technology and stored in platelet additive solutions
    Daniel R Ambruso
    Bonfils Blood Center and the Center for Cancer and Blood Disorders, The Children s Hospital, Denver, Colorado, USA
    Transfusion 51:1220-7. 2011
  10. doi Peroxiredoxin-6 and NADPH oxidase activity
    Daniel R Ambruso
    Department of Pediatrics, University of Colorado Denver, Anschutz Medical Campus, Aurora, Colorado, USA
    Methods Enzymol 527:145-67. 2013

Collaborators

  • Lynn K Boshkov
  • Gary L Johnson
  • Carlton C Barnett
  • Thomas L Leto
  • Steven M Anderson
  • Mark A Lovell
  • Michael A Ellison
  • Raymond P Goodrich
  • Amy N Abell
  • Christopher C Silliman
  • Gail Thurman
  • Abdulrahman Alsultan
  • David J Elzi
  • Patrick J Leavey
  • Urmish H Chudgar
  • Marsha S Anderson
  • Gail W Thurman
  • Marguerite R Kelher
  • Andrew J Paterson
  • Julie Zimbelman
  • Ronald J Sokol
  • Cindy Knall
  • Nancy Cusack
  • Don Mann
  • A Jason Bjornsen
  • Junichi Aiboshi
  • Kelly M England
  • Christine Hamiel
  • Marguerite Kelher
  • Zahra Mehdizadehkashi
  • Linda Podlosky
  • Nathan McLaughlin-Malaxecheberria
  • Travis H Wyman
  • Rene J Musters
  • Anirban Bannerjee
  • William O Dickey
  • Ronald J Harbeck
  • Gwen Clarke
  • Michael Kleinberg
  • Lori Rinckel
  • Stefanie Freeman
  • Frans A Kuypers
  • Carolina Gonzalez-Aller

Detail Information

Publications17

  1. doi Peroxiredoxin 6 translocates to the plasma membrane during neutrophil activation and is required for optimal NADPH oxidase activity
    Daniel R Ambruso
    Bonfils Blood Center, Denver, CO 80230, USA
    Biochim Biophys Acta 1823:306-15. 2012
    ..Prdx6 supports retention of the active oxidase complex in stimulated plasma membrane, and results with mutant proteins imply that Prdx6 serves an additional biochemical or structural role in supporting optimal NADPH oxidase activity...
  2. doi Lack of antibody formation to platelet neoantigens after transfusion of riboflavin and ultraviolet light-treated platelet concentrates
    Daniel R Ambruso
    Bonfils Blood Center, Denver, Colorado, USA
    Transfusion 49:2631-6. 2009
    ..This study evaluated serum of patients who received PRT-treated platelet (PLT) concentrates over a time period of 28 days for the appearance of antibodies to neoantigens on PLTs...
  3. ncbi Formyl-Met-Leu-Phe induces calcium-dependent tyrosine phosphorylation of Rel-1 in neutrophils
    Marguerite R Kelher
    Department of Surgery, University of Colorado Health Science Center School of Medicine, Denver, CO 80230, USA
    Cell Calcium 34:445-55. 2003
    ..Thus, fMLP concentrations that prime or activate the oxidase cause a rapid Ca2+-dependent tyrosine phosphorylation of Rel-1 involving p38 MAPK activation...
  4. ncbi NADPH oxidase activity of neutrophil specific granules: requirements for cytosolic components and evidence of assembly during cell activation
    Daniel R Ambruso
    Department of Pediatrics, The Bonfils Blood Center, The University of Colorado School of Medicine, Denver, CO, USA
    Mol Genet Metab 81:313-21. 2004
    ..These studies establish a critical role for specific granules as a site for assembly and activation of the oxidase enzyme system and an important constituent for the microbicidal activity of the neutrophil...
  5. ncbi CP-64131, an aminobenzazepine with cytokine-like properties, stimulates human neutrophil functions through the p38-MAPK pathway
    Marsha S Anderson
    Bonfils Blood Center, 717 Yosemite Street, Denver, CO 80230, USA
    J Leukoc Biol 76:477-83. 2004
    ..CP has the ability to activate specific MAPK pathways in different cell types and should prove to be an effective agonist in combination with inhibitors to study biological responses regulated by MAPKs...
  6. ncbi A 29-kDa protein associated with p67phox expresses both peroxiredoxin and phospholipase A2 activity and enhances superoxide anion production by a cell-free system of NADPH oxidase activity
    Patrick J Leavey
    Bonfils Blood Center, Denver, Colorado 80230, USA
    J Biol Chem 277:45181-7. 2002
    ..Thus, p29 is a novel protein that associates with p67 and has peroxiredoxin activity. This protein has a potential role in protecting the NADPH oxidase by inactivating H(2)O(2) or altering signaling pathways affected by H(2)O(2)...
  7. pmc Therapeutic options for transfusion related acute lung injury; the potential of the G2A receptor
    Michael A Ellison
    Belle Bonfils Memorial Blood Center, Denver, CO 80230, USA
    Curr Pharm Des 18:3255-9. 2012
    ..Additionally, characterization of the process by which lyso-PCs are generated in stored blood products could allow development of inhibitors and additive solutions to block their formation in the first place...
  8. ncbi Oxidase activity in cord blood neutrophils: a balance between increased membrane associated cytochrome b558 and deficient cytosolic components
    Urmish H Chudgar
    Department of Pediatrics, University of Colorado School of Medicine, 4200 East Ninth Avenue B128, Denver, CO 80262, USA
    Pediatr Blood Cancer 45:311-7. 2005
    ..Alterations in the function of neonatal phagocytes, including the activity of the neutrophil NADPH oxidase, have been suggested as one cause of increased susceptibility to such infections...
  9. doi Generation of neutrophil priming activity by cell-containing blood components treated with pathogen reduction technology and stored in platelet additive solutions
    Daniel R Ambruso
    Bonfils Blood Center and the Center for Cancer and Blood Disorders, The Children s Hospital, Denver, Colorado, USA
    Transfusion 51:1220-7. 2011
    ..We evaluated the priming activity of pathogen reduction technology (PRT)-treated PCs stored in plasma or platelet additive solution (PAS) and PRT-treated RBCs...
  10. doi Peroxiredoxin-6 and NADPH oxidase activity
    Daniel R Ambruso
    Department of Pediatrics, University of Colorado Denver, Anschutz Medical Campus, Aurora, Colorado, USA
    Methods Enzymol 527:145-67. 2013
    ..With these approaches, the role of Prdx6 in Nox2 activity can be explored with intact cells. The biochemical mechanisms of the Prdx6 effect on the NADPH oxidase can be investigated with the experimental strategies described. ..
  11. doi Phox activity of differentiated PLB-985 cells is enhanced, in an agonist specific manner, by the PLA2 activity of Prdx6-PLA2
    Michael A Ellison
    Bonfils Blood Center, Denver, CO 80230, USA
    Eur J Immunol 42:1609-17. 2012
    ....
  12. ncbi Lysophosphatidylcholines prime the NADPH oxidase and stimulate multiple neutrophil functions through changes in cytosolic calcium
    Christopher C Silliman
    Bonfils Blood Center, Denver, Colorado 80230, USA
    J Leukoc Biol 73:511-24. 2003
    ..In conclusion, lyso-PCs affect multiple PMN functions in a Ca(2)(+)-dependent manner that involves the activation of a pertussis toxin-sensitive G-protein...
  13. ncbi Transfusion-related acute lung injury
    Christopher C Silliman
    Bonfils Blood Center, 717 Yosemite St, Denver, CO 80230, USA
    Blood 105:2266-73. 2005
    ..Prevention, treatment, and a proposed definition of TRALI, especially in the context of ALI, are also examined...
  14. doi Long term G-CSF-induced remission of ulcerative colitis-like inflammatory bowel disease in a patient with glycogen storage disease Ib and evaluation of associated neutrophil function
    Abdulrahman Alsultan
    Department of Pediatrics and Pathology, University of Colorado Denver, Aurora, Colorado, USA
    Pediatr Blood Cancer 55:1410-3. 2010
    ..Ulcerative colitis-like IBD may also be seen in GSD Ib and is responsive to G-CSF therapy. Neutrophil dysfunction is variable among patients with GSD Ib...
  15. ncbi Rac2D57N, a dominant inhibitory Rac2 mutant that inhibits p38 kinase signaling and prevents surface ruffling in bone-marrow-derived macrophages
    Amy N Abell
    Department of Pharmacology, University of Colorado Health Sciences Center, 4200 East Ninth Ave, Denver, CO 80262, USA
    J Cell Sci 117:243-55. 2004
    ..Enhanced binding of Rac2D57N to its upstream regulators would inhibit Rac-dependent effects on actin cytoskeletal dynamics and p38 kinase signaling...
  16. ncbi Transfusion-related acute lung injury: epidemiology and a prospective analysis of etiologic factors
    Christopher C Silliman
    Bonfils Blood Center and the Department of Pediatrics, University of Colorado School of Medicine, Denver, USA
    Blood 101:454-62. 2003
    ..TRALI, like the acute respiratory distress syndrome, may be a 2-event phenomenon with both recipient predisposition and factors in the stored units playing major roles...
  17. ncbi In vivo treatment with granulocyte colony-stimulating factor does not delay apoptosis in human neutrophils by increasing the expression of the vacuolar proton ATPase
    Julie Zimbelman
    Bonfils Blood Center, Department of Pediatrics, University of Colorado School of Medicine, Denver 80230, USA
    J Investig Med 50:33-7. 2002
    ..Because this enzyme requires assembly of membrane and cytosolic domains to function, we hypothesized that in vivo G-CSF would increase synthesis and assembly of v-ATPase components to delay apoptosis...