Kari Airenne

Summary

Affiliation: University of Eastern Finland
Location: Kuopio, Finland
Grants:
Baculovirus technology, gene delivery, gene therapy

Publications

  1. ncbi Baculoviruses mediate efficient gene expression in a wide range of vertebrate cells
    Kari J Airenne
    Department of Molecular Medicine, A I Virtanen Institute, University of Eastern Finland, Kuopio, Finland
    Methods Mol Biol 737:279-301. 2011
  2. ncbi Baculovirus is an efficient vector for the transduction of the eye: comparison of baculovirus- and adenovirus-mediated intravitreal vascular endothelial growth factor D gene transfer in the rabbit eye
    Kati Kinnunen
    Department of Biotechnology and Molecular Medicine, A I Virtanen Institute, University of Kuopio, Kuopio, Finland
    J Gene Med 11:382-9. 2009
  3. ncbi Targeting and purification of metabolically biotinylated baculovirus
    Minna U Kaikkonen
    Department of Biotechnology and Molecular Medicine, A I Virtanen Institute for Molecular Sciences, FIN 70211 Kuopio, Finland
    Hum Gene Ther 19:589-600. 2008
  4. ncbi Generation of lentivirus vectors using recombinant baculoviruses
    H P Lesch
    Department of Biotechnology and Molecular Medicine, AI Virtanen Institute for Molecular Sciences, University of Kuopio, Kuopio, Finland
    Gene Ther 15:1280-6. 2008
  5. ncbi Baculovirus-mediated immediate-early gene expression and nuclear reorganization in human cells
    Johanna P Laakkonen
    Nanoscience Center, Department of Biological and Environmental Science, University of Jyvaskyla, Jyvaskyla, Finland
    Cell Microbiol 10:667-81. 2008
  6. ncbi Post-transcriptional regulatory element boosts baculovirus-mediated gene expression in vertebrate cells
    Anssi J Mähönen
    A I Virtanen Institute, Department of Biotechnology and Molecular Medicine, Kuopio, Finland
    J Biotechnol 131:1-8. 2007
  7. ncbi Non-invasive Imaging in Gene Therapy
    Jani Kristian Räty
    Department of Biotechnology and Molecular Medicine, A I Virtanen Institute for Molecular Sciences, University of Kuopio, Kuopio, Finland
    Mol Ther 15:1579-86. 2007
  8. ncbi Rhizavidin from Rhizobium etli: the first natural dimer in the avidin protein family
    Satu H Helppolainen
    Institute of Medical Technology, University of Tampere, FI 33014 Tampere, Finland
    Biochem J 405:397-405. 2007
  9. ncbi SPECT/CT imaging of baculovirus biodistribution in rat
    J K Räty
    Department of Biotechnology and Molecular Medicine, AI Virtanen Institute for Molecular Sciences, University of Kuopio, Kuopio, Finland
    Gene Ther 14:930-8. 2007
  10. ncbi Short and long-term effects of hVEGF-A(165) in Cre-activated transgenic mice
    Pia Leppänen
    Department of Biotechnology and Molecular Medicine, A I Virtanen Institute, University of Kuopio, Finland
    PLoS ONE 1:e13. 2006

Collaborators

Detail Information

Publications51

  1. ncbi Baculoviruses mediate efficient gene expression in a wide range of vertebrate cells
    Kari J Airenne
    Department of Molecular Medicine, A I Virtanen Institute, University of Eastern Finland, Kuopio, Finland
    Methods Mol Biol 737:279-301. 2011
    ....
  2. ncbi Baculovirus is an efficient vector for the transduction of the eye: comparison of baculovirus- and adenovirus-mediated intravitreal vascular endothelial growth factor D gene transfer in the rabbit eye
    Kati Kinnunen
    Department of Biotechnology and Molecular Medicine, A I Virtanen Institute, University of Kuopio, Kuopio, Finland
    J Gene Med 11:382-9. 2009
    ..We also studied how an intravitreal injection of vectors producing vascular endothelial growth factor D (VEGF-D) impacts the vasculature of rabbit eye...
  3. ncbi Targeting and purification of metabolically biotinylated baculovirus
    Minna U Kaikkonen
    Department of Biotechnology and Molecular Medicine, A I Virtanen Institute for Molecular Sciences, FIN 70211 Kuopio, Finland
    Hum Gene Ther 19:589-600. 2008
    ..These results demonstrate the utility of metabolically biotinylated baculovirus for vector targeting and viral purification applications...
  4. ncbi Generation of lentivirus vectors using recombinant baculoviruses
    H P Lesch
    Department of Biotechnology and Molecular Medicine, AI Virtanen Institute for Molecular Sciences, University of Kuopio, Kuopio, Finland
    Gene Ther 15:1280-6. 2008
    ..Our results show that baculoviruses are an attractive alternative for the production of lentiviruses in mammalian cells...
  5. ncbi Baculovirus-mediated immediate-early gene expression and nuclear reorganization in human cells
    Johanna P Laakkonen
    Nanoscience Center, Department of Biological and Environmental Science, University of Jyvaskyla, Jyvaskyla, Finland
    Cell Microbiol 10:667-81. 2008
    ..To conclude, this study shows that the non-replicative insect virus, baculovirus and its proteins can induce multiple changes in the cellular machinery of human cells...
  6. ncbi Post-transcriptional regulatory element boosts baculovirus-mediated gene expression in vertebrate cells
    Anssi J Mähönen
    A I Virtanen Institute, Department of Biotechnology and Molecular Medicine, Kuopio, Finland
    J Biotechnol 131:1-8. 2007
    ..Thus, inclusion of the WPRE into a baculovirus vector provides a simple means to improve BV-mediated gene expression in vertebrate cells...
  7. ncbi Non-invasive Imaging in Gene Therapy
    Jani Kristian Räty
    Department of Biotechnology and Molecular Medicine, A I Virtanen Institute for Molecular Sciences, University of Kuopio, Kuopio, Finland
    Mol Ther 15:1579-86. 2007
    ..This review describes the methodologies in use and highlights recent key approaches using the latest imaging modalities in gene therapy. Future trends in gene therapy imaging are also discussed...
  8. ncbi Rhizavidin from Rhizobium etli: the first natural dimer in the avidin protein family
    Satu H Helppolainen
    Institute of Medical Technology, University of Tampere, FI 33014 Tampere, Finland
    Biochem J 405:397-405. 2007
    ..Similarly to avidin, rhizavidin was observed to accumulate rapidly, mainly in the liver. Evidently, rhizavidin could be used as a complement to (strept)avidin in (strept)avidin-biotin technology...
  9. ncbi SPECT/CT imaging of baculovirus biodistribution in rat
    J K Räty
    Department of Biotechnology and Molecular Medicine, AI Virtanen Institute for Molecular Sciences, University of Kuopio, Kuopio, Finland
    Gene Ther 14:930-8. 2007
    ..Additionally, transduction of kidneys was seen with i.m. and i.f. administrations. We conclude that baculovirus may be beneficial for the treatment of kidney diseases after i.p. administration route...
  10. ncbi Short and long-term effects of hVEGF-A(165) in Cre-activated transgenic mice
    Pia Leppänen
    Department of Biotechnology and Molecular Medicine, A I Virtanen Institute, University of Kuopio, Finland
    PLoS ONE 1:e13. 2006
    ..These findings have implications for the design of long-term clinical trials using hVEGF-A(165) gene and protein...
  11. ncbi Magnetic resonance imaging of viral particle biodistribution in vivo
    J K Räty
    Department of Biotechnology and Molecular Medicine, University of Kuopio, Kuopio, Finland
    Gene Ther 13:1440-6. 2006
    ..Considering the widespread use of MRI in current medical imaging, the approach is likely to provide numerous future applications in imaging of therapeutic gene transfer...
  12. ncbi Clathrin-independent entry of baculovirus triggers uptake of E. coli in non-phagocytic human cells
    Johanna P Laakkonen
    Department of Biological and Environmental Science Nanoscience Center, University of Jyvaskyla, Jyvaskyla, Finland
    PLoS ONE 4:e5093. 2009
    ..This study increases our understanding of virus entry strategies and gives new insight into baculovirus-mediated gene delivery in human cells...
  13. ncbi Novel vascular endothelial growth factor D variants with increased biological activity
    Pyry I Toivanen
    Department of Biotechnology and Molecular Medicine, A I Virtanen Institute for Molecular Sciences, FI 70211 Kuopio, Finland
    J Biol Chem 284:16037-48. 2009
    ..The novel variants of VEGF-D(DeltaNDeltaC) described here are potential agents for therapeutic applications, where induction of vascular formation is required...
  14. ncbi A 96-well format for a high-throughput baculovirus generation, fast titering and recombinant protein production in insect and mammalian cells
    Hanna Riikka Kärkkäinen
    Department of Biotechnology and Molecular Medicine, A I Virtanen Institute for Molecular Sciences, Kuopio, Finland
    BMC Res Notes 2:63. 2009
    ..Baculovirus expression vector system (BEVS) has become a standard in recombinant protein production and virus-like particle preparation for numerous applications...
  15. ncbi Production and purification of lentiviral vectors generated in 293T suspension cells with baculoviral vectors
    H P Lesch
    A I Virtanen Institute, Department of Biotechnology and Molecular Medicine, University of Eastern Finland, Kuopio, Finland
    Gene Ther 18:531-8. 2011
    ..Furthermore, a scalable and cost-effective capture purification step was developed based on a diethylaminoethyl monolithic column capable of removing most of the baculoviruses from the LV pool with 65% recovery...
  16. ncbi In vivo application and tracking of baculovirus
    Kari J Airenne
    AI Virtanen Institute, Department of Molecular Medicine, University of Eastern Finland, FI 70211 Kuopio, Finland
    Curr Gene Ther 10:187-94. 2010
    ..This review summarizes the pre-clinical in vivo work carried out with baculoviruses and discusses remaining challenges which still need to be solved...
  17. ncbi Screening of complement inhibitors: shielded baculoviruses increase the safety and efficacy of gene delivery
    Minna U Kaikkonen
    Department of Biotechnology and Molecular Medicine, AI Virtanen Institute, University of Eastern Finland, Kuopio, Finland
    Mol Ther 18:987-92. 2010
    ..In conclusion, complement shielded baculovirus vectors represent attractive tools for effective in vivo gene delivery...
  18. ncbi Transduction of vertebrate cells with recombinant baculovirus
    Kari J Airenne
    Cold Spring Harb Protoc 2009:pdb.prot5182. 2009
  19. ncbi Preparation of recombinant baculoviruses with the BVboost system
    Kari J Airenne
    Cold Spring Harb Protoc 2009:pdb.prot5181. 2009
  20. ncbi Production of HIV-1 integrase fusion protein-carrying lentiviral vectors for gene therapy and protein transduction
    Diana Schenkwein
    Department of Biotechnology and Molecular Medicine, A I Virtanen Institute for Molecular Sciences, University of Kuopio, FIN 70211 Kuopio, Finland
    Hum Gene Ther 21:589-602. 2010
    ....
  21. ncbi Morphological characterization of baculovirus Autographa californica multiple nucleopolyhedrovirus
    Teemu O Ihalainen
    Nanoscience Center, Department of Biological and Environmental Science, University of Jyvaskyla, Jyvaskyla, Finland
    Virus Res 148:71-4. 2010
    ..Taken together, our results provide the first 3D-characterization of baculovirus morphology and insight into its stability after storage...
  22. ncbi Culture medium induced vimentin reorganization associates with enhanced baculovirus-mediated gene delivery
    Anssi J Mähönen
    A I Virtanen Institute, Department of Biotechnology and Molecular Medicine, University of Kuopio Kuopio, Finland
    J Biotechnol 145:111-9. 2010
    ..The results highlight the general importance of the culture medium in the assembly of the cytoskeleton network and in viral gene delivery...
  23. ncbi (Strept)avidin-displaying lentiviruses as versatile tools for targeting and dual imaging of gene delivery
    M U Kaikkonen
    Department of Biotechnology and Molecular Medicine, AI Virtanen Institute for Molecular Sciences, University of Kuopio, Kuopio, Finland
    Gene Ther 16:894-904. 2009
    ..This study suggests that these novel vectors could be applicable for the treatment of central nervous system disorders and cancer...
  24. ncbi Avidin fusion protein-expressing lentiviral vector for targeted drug delivery
    Hanna P Lesch
    Department of Biotechnology and Molecular Medicine, A I Virtanen Institute, FIN 70210 Kuopio, Finland
    Hum Gene Ther 20:871-82. 2009
    ..The lentivirus-mediated delivery of the avidin fusion protein thus represents a potential approach for the repeated targeting of cytotoxic compounds to cancer cells...
  25. ncbi Truncated vesicular stomatitis virus G protein improves baculovirus transduction efficiency in vitro and in vivo
    M U Kaikkonen
    AI Virtanen Institute, Department of Biotechnology and Molecular Medicine, University of Kuopio, Kuopio, Finland
    Gene Ther 13:304-12. 2006
    ..Moreover, no cytotoxicity was associated with improved gene transfer efficiency. Thus, VSV-GED pseudotyping provides a simple means to enhance baculovirus-mediated gene transfer in vitro and in vivo...
  26. ncbi A multipurpose vector system for the screening of libraries in bacteria, insect and mammalian cells and expression in vivo
    Olli H Laitinen
    Department of Molecular Medicine and Biotechnology, A.I. Virtanen Institute, University of Kuopio PO Box 1627 FIN-70211, Kuopio, Finland
    Nucleic Acids Res 33:e42. 2005
    ..The pBVboostFG enables screening of genome-wide libraries, thus making it an efficient new platform technology for functional genomics...
  27. ncbi Improvement in nuclear entry and transgene expression of baculoviruses by disintegration of microtubules in human hepatocytes
    Mirka Salminen
    Department of Biological and Environmental Science, P.O. Box 35 (YAB, , , Finland
    J Virol 79:2720-8. 2005
    ..Together, these data suggest that the intact MTs constitute a barrier to baculovirus transport toward the nucleus...
  28. ncbi Baculovirus-mediated periadventitial gene transfer to rabbit carotid artery
    K J Airenne
    AI Virtanen Institute, Department of Molecular Medicine, University of Kuopio, Finland
    Gene Ther 7:1499-504. 2000
    ..Baculoviruses are thus able to mediate transient gene transfer in vivo and may become useful tools for gene therapy...
  29. ncbi Selective targeting of avidin/mannose 6-phosphate receptor chimeras to early or late endosomes
    K Juuti-Uusitalo
    University of Jyvaskyla, Department of Biological and Environmental Science, Finland
    Eur J Cell Biol 79:458-68. 2000
    ..Our results also suggest that the YKYSKV sequence close to the CI-MPR transmembrane segment is sufficient for targeting to sorting early endosomes...
  30. ncbi Recombinant avidin and avidin-fusion proteins
    K J Airenne
    Department of Biological and Environmental Science, University of Jyvaskyla, Finland
    Biomol Eng 16:87-92. 1999
    ..This review article presents an overview of the current status of these systems. Future trends in the production and applications of recombinant avidin and avidin-fusion proteins are also discussed...
  31. ncbi Recombinant NeutraLite avidin: a non-glycosylated, acidic mutant of chicken avidin that exhibits high affinity for biotin and low non-specific binding properties
    A T Marttila
    Department of Biological and Environmental Science, University of Jyvaskyla, 40 351, Jyvaskyla, Finland
    FEBS Lett 467:31-6. 2000
    ..Due to its acidic pI, it is relatively free from non-specific binding to DNA and cells. The recombinant NeutraLite Avidin retains seven lysines per subunit, which are available for further conjugation and derivatization...
  32. ncbi Mutation of a critical tryptophan to lysine in avidin or streptavidin may explain why sea urchin fibropellin adopts an avidin-like domain
    O H Laitinen
    Department of Biological and Environmental Science, University of Jyvaskyla, FIN 40351, Jyvaskyla, Finland
    FEBS Lett 461:52-8. 1999
    ..These findings may indicate that mimicry in the nature of the avidin sequence and fold by the fibropellins is not designed to generate biotin-binding, but may serve to secure an appropriate structure for facilitating dimerization...
  33. ncbi Avidin is a promising tag for fusion proteins produced in baculovirus-infected insect cells
    K J Airenne
    Department of Biological and Environmental Science, University of Jyvaskyla, Jyvaskyla, FIN 40100, Finland
    Protein Expr Purif 17:139-45. 1999
    ..The versatility with which avidin fusion proteins can be detected, purified, and immobilized is the basis for the use of our system as a useful alternative in eukaryotic fusion protein production...
  34. ncbi Local hypomethylation in atherosclerosis found in rabbit ec-sod gene
    M O Laukkanen
    A I Virtanen Institute, University of Kuopio, Kuopio, Finland
    Arterioscler Thromb Vasc Biol 19:2171-8. 1999
    ....
  35. ncbi Engineering of chicken avidin: a progressive series of reduced charge mutants
    A T Marttila
    Department of Biological and Environmental Science, University of Jyvaskyla, Finland
    FEBS Lett 441:313-7. 1998
    ..Our results demonstrate that the charge properties of avidin can be modified without disturbing the crucial biotin-binding activity...
  36. ncbi Production of biologically active recombinant avidin in baculovirus-infected insect cells
    K J Airenne
    Department of Biological and Environmental Science, University of Jyvaskyla, Finland
    Protein Expr Purif 9:100-8. 1997
    ....
  37. ncbi Rapid purification of recombinant proteins fused to chicken avidin
    K J Airenne
    Department of Biological and Environmental Science, University of Jyvaskyla, Finland
    Gene 167:63-8. 1995
    ..coli, biologically active fusion proteins consisting of Avd C- or N-terminally linked with the desired protein which then can easily be purified by a simple affinity chromatography procedure...
  38. ncbi Biotin induces tetramerization of a recombinant monomeric avidin. A model for protein-protein interactions
    O H Laitinen
    Department of Biological and Environmental Science, , FIN-40351, , Finland
    J Biol Chem 276:8219-24. 2001
    ..This study may suggest a biological role for low molecular weight ligands in inducing oligomerization and in maintaining the stability of multimeric protein assemblies...
  39. ncbi Cloning and characterization of Scavidin, a fusion protein for the targeted delivery of biotinylated molecules
    Pauliina Lehtolainen
    A. I. Virtanen Institute, University of Kuopio, FIN-70211 Kuopio, Finland
    J Biol Chem 277:8545-50. 2002
    ....
  40. ncbi Chicken avidin-related proteins show altered biotin-binding and physico-chemical properties as compared with avidin
    Olli H Laitinen
    Department of Biological and Environmental Science, , P.O. Box 35, , Finland
    Biochem J 363:609-17. 2002
    ..These, together with the other properties of the AVRs described in the present paper, may offer advantages over avidin and streptavidin, making the AVRs applicable for improved avidin-biotin technological applications...
  41. ncbi Efficient production of active chicken avidin using a bacterial signal peptide in Escherichia coli
    Vesa P Hytönen
    Department of Biological and Environmental Science, Nanoscience Center, P O Box 35 YAB, FIN 40014 University of Jyväskylä, Finland
    Biochem J 384:385-90. 2004
    ..Nucl. Med. 37, 1380-1384]. The present method provides a feasible and inexpensive alternative for the production of recombinant avidin, avidin mutants and avidin fusion proteins for novel avidin-biotin technology applications...
  42. ncbi Baculovirus display strategies: Emerging tools for eukaryotic libraries and gene delivery
    Christian Oker-Blom
    University of Jyväskylä in Finland
    Brief Funct Genomic Proteomic 2:244-53. 2003
    ..Finally, the viruses can accommodate large insertions of foreign DNA and replicate only in insect cells. Together, these are attributes that are very likely to make them important tools in functional genomics and proteomics...
  43. ncbi Optimized self-excising Cre-expression cassette for mammalian cells
    Anssi J Mähönen
    Department of Molecular Medicine and Biotechnology, AI Virtanen Institute, University of Kuopio, Kuopio, Finland
    Biochem Biophys Res Commun 320:366-71. 2004
    ..SSi-Cre should thus provide a useful tool for various applications based on the Cre/loxP system...
  44. ncbi Construction of hevein (Hev b 6.02) with reduced allergenicity for immunotherapy of latex allergy by comutation of six amino acid residues on the conformational IgE epitopes
    Piia Karisola
    Department of Biological and Environmental Science, , , Finland
    J Immunol 172:2621-8. 2004
    ..Hevein with a highly reduced ability to bind IgE should provide a valuable candidate molecule for immunotherapy of latex allergy and is anticipated to have a low risk of systemic side effects...
  45. ncbi Enhanced gene delivery by avidin-displaying baculovirus
    Jani K Raty
    A I Virtanen Institute, Department of Biotechnology and Molecular Medicine, University of Kuopio, P O Box 1627, FIN 70120 Kuopio, Finland
    Mol Ther 9:282-91. 2004
    ..Altogether, we show that avidin display is a rapid and versatile method to improve viral properties for gene delivery...
  46. ncbi Baculovirus capsid display: a novel tool for transduction imaging
    Sari P Kukkonen
    A. I. Virtanen Institute, Department of Molecular Medicine and Biotechnology, FIN-70211, Kuopio, Finland
    Mol Ther 8:853-62. 2003
    ..Furthermore, capsid-modified baculoviruses hold great promise for the nuclear and subcellular targeting of transgenes and as a novel peptide display system for a variety of eukaryotic applications...
  47. ncbi Targeting of biotinylated compounds to its target tissue using a low-density lipoprotein receptor-avidin fusion protein
    P Lehtolainen
    A.I. Virtanen Institute, University of Kuopio, Kuopio, Finland
    Gene Ther 10:2090-7. 2003
    ..These results suggest that local gene transfer of the fusion protein to target tissues may offer a novel tool for the delivery of biotinylated molecules in vitro and in vivo for therapeutic and imaging purposes...
  48. ncbi Improved generation of recombinant baculovirus genomes in Escherichia coli
    Kari J Airenne
    AI Virtanen Institute, Department of Molecular Medicine and Biotechnology, University of Kuopio and Kuopio University Hospital, Kuopio, Finland
    Nucleic Acids Res 31:e101. 2003
    ..The introduced selection scheme is also useful for the construction of other vectors by transposition in E.coli...
  49. ncbi Rational design of an active avidin monomer
    Olli H Laitinen
    Department of Biological and Environmental Science, P. O. Box 35, , Finland
    J Biol Chem 278:4010-4. 2003
    ..This new monomeric avidin, which binds biotin reversibly, may be useful for applications both in vitro and in vivo. It may also shed light on the effect of intersubunit interactions on the binding of ligands...
  50. ncbi Baculoviruses exhibit restricted cell type specificity in rat brain: a comparison of baculovirus- and adenovirus-mediated intracerebral gene transfer in vivo
    P Lehtolainen
    AI Virtanen Institute and Gene Therapy Unit, Kuopio University Hospital, Finland
    Gene Ther 9:1693-9. 2002
    ..The knowledge of the cell type specificity of the vectors may offer a possibility to achieve targeted gene delivery to distinct brain areas. Baculoviruses seem to be especially useful for the targeting of choroid plexus cells...
  51. ncbi Production of recombinant avidin in Escherichia coli
    K J Airenne
    Department of Biology, University of Jyvaskyla, Finland
    Gene 144:75-80. 1994
    ..coli which then can easily be purified by affinity chromatography on a biotin column in a single step...