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Genomes and GenesSpecies | REV3SummaryGene Symbol: REV3 Description: Rev3p Alias: PSO1 Species: Top Publications
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The RAD6 DNA damage tolerance pathway operates uncoupled from the replication fork and is functional beyond S phaseGeorgios I Karras
Department of Molecular Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
Cell 141:255-67. 2010..We therefore propose that the RAD6 pathway acts on single-stranded gaps left behind newly restarted replication forks...- Ubiquitin-dependent DNA damage bypass is separable from genome replicationYasukazu Daigaku
Cancer Research UK London Research Institute, Clare Hall Laboratories, Blanche Lane, South Mimms EN6 3LD, UK
Nature 465:951-5. 2010..Our approach has revealed the distribution of PRR tracts in a synchronized cell population. It will allow an in-depth mechanistic analysis of how cells manage the processing of lesions to their genomes during and after replication... - Control of spontaneous and damage-induced mutagenesis by SUMO and ubiquitin conjugationPhilipp Stelter
Max Planck Institute for Terrestrial Microbiology, Karl von Frisch Strasse, 35043 Marburg, Germany
Nature 425:188-91. 2003..Our findings assign a function to SUMO during S phase and demonstrate how ubiquitin and SUMO, by regulating the accuracy of replication and repair, contribute to overall genomic stability... - PCNA mono-ubiquitination and activation of translesion DNA polymerases by DNA polymerase {alpha}Motoshi Suzuki
Center for Neurological Diseases and Cancer, Nagoya University Graduate School of Medicine, Japan
J Biochem 146:13-21. 2009..These data suggest that nucleotide misincorporation by pol alpha induces exposure of single-stranded DNA, PCNA mono-ubiquitination and activates TLS pols... - Requirement of RAD52 group genes for postreplication repair of UV-damaged DNA in Saccharomyces cerevisiaeVenkateswarlu Gangavarapu
University of Texas Medical Branch at Galveston, 301 University Blvd, Galveston, TX 77555 1061, USA
Mol Cell Biol 27:7758-64. 2007..In addition, our results suggest a role for the Rad50 and Xrs2 proteins and thereby for the MRX complex in promoting PRR via both the Rad5 and Rad52 pathways... - The 9-1-1 checkpoint clamp physically interacts with polzeta and is partially required for spontaneous polzeta-dependent mutagenesis in Saccharomyces cerevisiaeSimone Sabbioneda
Dipartimento di Scienze Biomolecolari e Biotecnologie, Universita degli Studi di Milano, Milano, Italy 20133
J Biol Chem 280:38657-65. 2005..These results suggest that, in addition to its checkpoint signaling role, the 9-1-1 clamp may physically regulate Polzeta-dependent mutagenesis by controlling the access of Polzeta to damaged DNA... - Roles of RAD6 epistasis group members in spontaneous polzeta-dependent translesion synthesis in Saccharomyces cerevisiaeBrenda K Minesinger
Biochemistry, Cell and Developmental Biology Program of the Graduate Division of Biological and Biomedical Sciences, Emory University, Atlanta, GA 30322, USA
Genetics 169:1939-55. 2005..A third error-free pathway relies on the presence of Mms2, but may not require PCNA... - The relative roles in vivo of Saccharomyces cerevisiae Pol eta, Pol zeta, Rev1 protein and Pol32 in the bypass and mutation induction of an abasic site, T-T (6-4) photoadduct and T-T cis-syn cyclobutane dimerPeter E M Gibbs
Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
Genetics 169:575-82. 2005..an abasic site, T-T (6-4) photoadduct and T-T cis-syn cyclobutane dimer, by transforming strains deleted for RAD30, REV3, REV1, or POL32 with duplex plasmids carrying one of these DNA lesions located within a 28-nucleotide single-.. - A role for yeast and human translesion synthesis DNA polymerases in promoting replication through 3-methyl adenineRobert E Johnson
Department of Biochemistry and Molecular Biology, University of Texas Medical Branch at Galveston, 301 University Blvd, Galveston, TX 77555 1061, USA
Mol Cell Biol 27:7198-205. 2007..We discuss these results in the context of previous observations that have been made for the roles of Pols eta, iota, and kappa in promoting replication through the minor-groove N2-dG adducts... - The mismatch repair system promotes DNA polymerase zeta-dependent translesion synthesis in yeastKevin Lehner
Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA
Proc Natl Acad Sci U S A 106:5749-54. 2009..Finally, in contrast to its ability to remove mistakes made by replicative DNA polymerases, we show that MMR fails to efficiently correct errors introduced by Polzeta... - Role of DNA polymerase eta in the bypass of abasic sites in yeast cellsBo Zhao
Graduate Center for Toxicology, University of Kentucky, Lexington, KY 40536, USA
Nucleic Acids Res 32:3984-94. 2004..In cells lacking Poleta (rad30), Rev1, Polzeta (rev3), and both Poleta and Polzeta, translesion synthesis was reduced to 30%, 30%, 15% and 3% of the wild-type level, .. - The yeast Shu complex couples error-free post-replication repair to homologous recombinationLindsay G Ball
Department of Microbiology and Immunology, University of Saskatchewan, Saskatoon, SK, Canada S7N 5E5
Mol Microbiol 73:89-102. 2009..This mechanism appears to be conserved throughout eukaryotes... - The effect of oxidative metabolism on spontaneous Pol zeta-dependent translesion synthesis in Saccharomyces cerevisiaeBrenda K Minesinger
Biochemistry, Cell and Developmental Biology Program of the Graduate Division of Biological and Biomedical Sciences, Emory University, Atlanta, GA 30322, USA
DNA Repair (Amst) 5:226-34. 2006..These results suggest that translesion bypass of spontaneously oxidized DNA bases can be a significant source of mutagenesis in repair compromised cells... - Hypermutability of damaged single-strand DNA formed at double-strand breaks and uncapped telomeres in yeast Saccharomyces cerevisiaeYong Yang
Department of Health and Human Services, Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina, United States of America
PLoS Genet 4:e1000264. 2008..Hypermutability and multiple mutations associated with lesions in transient stretches of long single-strand DNA may be a source of carcinogenesis and provide selective advantage in adaptive evolution... - Identification of a strand-related bias in the PCNA-mediated bypass of spontaneous lesions by yeast PoletaAmy L Abdulovic
Graduate Program in Biochemistry, Cell and Developmental Biology Program, Emory University, Atlanta, GA 30322, United States
DNA Repair (Amst) 6:1307-18. 2007..Our results suggest that there is a polymerase hierarchy between Poleta and Polzeta in the bypass of certain lesions and that the interaction of Poleta with PCNA is needed for some, but not all, spontaneous lesion bypass... - Mutator alleles of yeast DNA polymerase zetaAyako N Sakamoto
Research Group for Gene Resources, Department of Ion Beam Applied Biology, Japan Atomic Energy Agency, Watanuki machi 1233, Takasaki, Gunma 370 1292, Japan
DNA Repair (Amst) 6:1829-38. 2007The yeast REV3 gene encodes the catalytic subunit of DNA polymerase zeta (pol zeta), a B family polymerase that performs mutagenic DNA synthesis in cells... - DNA damage checkpoints are involved in postreplication repairLeslie Barbour
Department of Microbiology and Immunology, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E5, Canada
Genetics 174:1789-800. 2006..variant, belongs to the error-free branch of the RAD6 postreplication repair (PRR) pathway, and is parallel to the REV3-mediated mutagenesis branch... - Complex formation with Rev1 enhances the proficiency of Saccharomyces cerevisiae DNA polymerase zeta for mismatch extension and for extension opposite from DNA lesionsNarottam Acharya
Sealy Center for Molecular Science, University of Texas Medical Branch at Galveston, Galveston, TX 77555 1061, USA
Mol Cell Biol 26:9555-63. 2006Rev1, a Y family DNA polymerase (Pol) functions together with Polzeta, a B family Pol comprised of the Rev3 catalytic subunit and Rev7 accessory subunit, in promoting translesion DNA synthesis (TLS)... - Genetic analysis of ionizing radiation-induced mutagenesis in Saccharomyces cerevisiae reveals TransLesion Synthesis (TLS) independent of PCNA K164 SUMOylation and ubiquitinationClark C Chen
Department of Neurosurgery, Massachusetts General Hospital, 55 Fruit St, Boston, MA 02114, USA
DNA Repair (Amst) 5:1475-88. 2006..Of the mutations inactivating TLS polymerases, rev3 and rev1 caused equally severe defects in IR-IM whereas rad30 did not significantly affect the process... - Functional and physical interaction of yeast Mgs1 with PCNA: impact on RAD6-dependent DNA damage toleranceTakashi Hishida
Genome Dynamics Group, Research Institute for Microbial Diseases, Osaka University, Yamadaoka 3 1, Suita, Osaka 565 0871, Japan
Mol Cell Biol 26:5509-17. 2006..The proposed roles for Mgs1, Srs2, and modified PCNA during replication arrest highlight the importance of modulating the RAD6 and RAD52 pathways to avoid genome instability... - The Saccharomyces cerevisiae Rad6 postreplication repair and Siz1/Srs2 homologous recombination-inhibiting pathways process DNA damage that arises in asf1 mutantsEllen S Kats
Ludwig Institute for Cancer Research, Departments of Medicine and Cellular and Molecular Medicine, and Biomedical Sciences Graduate Program, UC San Diego School of Medicine, 9500 Gilman Drive, La Jolla, CA 92093 0669, USA
Mol Cell Biol 29:5226-37. 2009..Our results show that ASF1 probably contributes to the maintenance of genome stability through multiple mechanisms, some of which involve the PRR and HRS pathways... - Anc1, a protein associated with multiple transcription complexes, is involved in postreplication repair pathway in S. cerevisiaeRachel L Erlich
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA
PLoS ONE 3:e3717. 2008..Anc1's role in the PRR pathway, as well as its role in suppressing triplet repeats, point to a possible mechanism for a protein of potential medical interest... - Identification of APN2, the Saccharomyces cerevisiae homolog of the major human AP endonuclease HAP1, and its role in the repair of abasic sitesR E Johnson
Sealy Center for Molecular Science, University of Texas Medical Branch, Galveston, Texas 77555 1061, USA
Genes Dev 12:3137-43. 1998..apn1Delta apn2Delta strain displays a highly elevated level of MMS-induced mutagenesis, which is dependent on the REV3, REV7, and REV1 genes... - Yeast DNA polymerase zeta (zeta) is essential for error-free replication past thymine glycolRobert E Johnson
Sealy Center for Molecular Science, University of Texas Medical Branch at Galveston, Galveston, Texas 77555 1061, USA
Genes Dev 17:77-87. 2003..Intriguingly, however, disruption of the REV3 gene, which encodes the catalytic subunit of Polzeta, causes early embryonic lethality in mice... - Suppression of genetic defects within the RAD6 pathway by srs2 is specific for error-free post-replication repair but not for damage-induced mutagenesisStacey Broomfield
Department of Microbiology and Immunology, University of Saskatchewan, 107 Wiggins Road, Saskatoon, SK S7N 5E5, Canada
Nucleic Acids Res 30:732-9. 2002..rather independent subpathways: two error-free (represented by RAD5 and POL30) and one error-prone (represented by REV3)... - Translesion synthesis by yeast DNA polymerase zeta from templates containing lesions of ultraviolet radiation and acetylaminofluoreneD Guo
Graduate Center for Toxicology, 306 Health Sciences Research Building, University of Kentucky, Lexington, KY 40536, USA
Nucleic Acids Res 29:2875-83. 2001..Secondly, more efficient bypass of these lesions may require nucleotide incorporation by other DNA polymerases followed by extension DNA synthesis by Polzeta... - Role of DNA polymerase zeta in the bypass of a (6-4) TT photoproductR E Johnson
Sealy Center for Molecular Science, University of Texas Medical Branch, Galveston, Texas 77555 1061, USA
Mol Cell Biol 21:3558-63. 2001.... - The Saccharomyces cerevisiae RAD6 group is composed of an error-prone and two error-free postreplication repair pathwaysW Xiao
Department of Microbiology and Immunology, University of Saskatchewan, Saskatoon, Saskatchewan, S7N 5E5 Canada
Genetics 155:1633-41. 2000..can be exclusively divided into three rather than two independent subpathways represented by the RAD5, POL30, and REV3 genes; the REV3 pathway is largely mutagenic, whereas the RAD5 and the POL30 pathways are deemed error free... - Genetic interactions between error-prone and error-free postreplication repair pathways in Saccharomyces cerevisiaeW Xiao
Department of Microbiology and Immunology, University of Saskatchewan, Saskatoon, Canada
Mutat Res 435:1-11. 1999..In addition, mutagenenic effects and genetic interactions of the mms2 mutator and rev3 anti-mutator were examined with respect to forward mutations, frameshift reversions as well as amber and ochre .. - MMS2, encoding a ubiquitin-conjugating-enzyme-like protein, is a member of the yeast error-free postreplication repair pathwayS Broomfield
Department of Microbiology, University of Saskatchewan, 107 Wiggins Road, Saskatoon, SK Canada S7N 5E5
Proc Natl Acad Sci U S A 95:5678-83. 1998..The rad6 and rad18 mutants are defective in both pathways, and the rev3 mutant affects only the mutagenesis pathway, but a yeast gene that is involved only in error-free postreplication .. - The Saccharomyces cerevisiae RAD30 gene, a homologue of Escherichia coli dinB and umuC, is DNA damage inducible and functions in a novel error-free postreplication repair mechanismJ P McDonald
Section on DNA Replication, Repair and Mutagenesis, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 2725, USA
Genetics 147:1557-68. 1997..While rad6 and rad18 are both epistatic to rad30, no epistasis was observed with rev1, rev3, rev7 or rad5, all of which are members of the RAD6 epistasis group... - Thymine-thymine dimer bypass by yeast DNA polymerase zetaJ R Nelson
Department of Biophysics, School of Medicine and Dentistry, University of Rochester Medical Center, New York 14642, USA
Science 272:1646-9. 1996The REV3 and REV7 genes of the yeast Saccharomyces cerevisiae are required for DNA damage-induced mutagenesis. The Rev3 and Rev7 proteins were shown to form a complex with DNA polymerase activity... - Specificity of the yeast rev3 delta antimutator and REV3 dependency of the mutator resulting from a defect (rad1 delta) in nucleotide excision repairH Roche
Department of Microbiology, University of Manitoba, Winnipeg, Canada
Genetics 137:637-46. 1994The yeast REV3 gene has been predicted to encode a DNA polymerase specializing in translesion synthesis. This polymerase likely participates in spontaneous mutagenesis, as rev3 mutants have an antimutator phenotype... - In vivo consequences of putative active site mutations in yeast DNA polymerases alpha, epsilon, delta, and zetaY I Pavlov
Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
Genetics 159:47-64. 2001..Strains with same substitution in Rev3, the catalytic subunit of Pol zeta, are nearly UV immutable, suggesting severe loss of function... - Dissection of the functions of the Saccharomyces cerevisiae RAD6 postreplicative repair group in mutagenesis and UV sensitivityP Cejka
Department of Genetics and Microbiology, Faculty of Natural Sciences, Charles University, 128 44 Prague, Czech Republic
Genetics 159:953-63. 2001..of strains expressing various alleles of the RAD6 gene and single and multiple mutants of the RAD6, RAD5, RAD18, REV3, and MMS2 genes from the RAD6 repair group... - Deletion of the MAG1 DNA glycosylase gene suppresses alkylation-induced killing and mutagenesis in yeast cells lacking AP endonucleasesW Xiao
Department of Microbiology and Immunology, University of Saskatchewan, Saskatoon, Saskatchewan, Canada S7N 5E5
Mutat Res 487:137-47. 2001..These results allow us to delineate base lesion flow within the BER pathway and link AP sites to other DNA damage repair and tolerance pathways... - The in vivo characterization of translesion synthesis across UV-induced lesions in Saccharomyces cerevisiae: insights into Pol zeta- and Pol eta-dependent frameshift mutagenesisAmy L Abdulovic
Graduate Division of Biological and Biomedical Sciences, Emory University, Atlanta, Georgia 30322, USA
Genetics 172:1487-98. 2006..Finally, the examination of frameshift reversion spectra indicates a hierarchy between Pol eta and Pol zeta with respect to the bypass of UV-induced lesions... - A novel function of DNA polymerase zeta regulated by PCNAMatthew R Northam
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, NE 68198, USA
EMBO J 25:4316-25. 2006.... - Pol32, a subunit of Saccharomyces cerevisiae DNA polymerase delta, suppresses genomic deletions and is involved in the mutagenic bypass pathwayMeng Er Huang
UMR6061 CNRS, Genetique et Developpement, Faculte de Medecine, 35043 Rennes, France
Genetics 160:1409-22. 2002..Here, by measuring the CAN1 forward mutation rate, we found that either POL32 or REV3 (which encodes the Pol zeta catalytic subunit) inactivation produces overlapping antimutator effects against rad .. - UBC13, a DNA-damage-inducible gene, is a member of the error-free postreplication repair pathway in Saccharomyces cerevisiaeJ Brusky
Department of Microbiology and Immunology, University of Saskatchewan, Saskatoon, Canada
Curr Genet 37:168-74. 2000..We also found that ubc13 is synergistic to the error-prone PRR pathway mutation rev3, indicating that UBC13 is in a pathway alternative to REV3 mutagenesis... - DNA polymerase zeta is essential for hexavalent chromium-induced mutagenesisTravis J O'Brien
Department of Pharmacology and Physiology, The George Washington University, Washington, DC 20037, USA
Mutat Res 663:77-83. 2009..Wild-type yeast and strains deficient in TLS polymerases (i.e. Polzeta (rev3), Poleta (rad30)) were exposed to Cr(VI) and monitored for cell survival and forward mutagenesis at the CAN1 locus... - dUTP incorporation into genomic DNA is linked to transcription in yeastNayun Kim
Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina 27710, USA
Nature 459:1150-3. 2009..These results show an unexpected relationship between transcription and the fidelity of DNA synthesis, and raise intriguing cell biological issues with regard to nucleotide pool compartmentalization... - Constitutive fusion of ubiquitin to PCNA provides DNA damage tolerance independent of translesion polymerase activitiesLandon Pastushok
Department of Microbiology and Immunology, University of Saskatchewan, Saskatoon, SK, Canada
Nucleic Acids Res 38:5047-58. 2010..Ub cause elevated spontaneous mutagenesis, which is a defining characteristic of REV3-dependent TLS activity... - Defects in DNA lesion bypass lead to spontaneous chromosomal rearrangements and increased cell deathKristina H Schmidt
Department of Cell Biology, Microbiology and Molecular Biology, University of South Florida, Tampa, FL 33620, USA
Eukaryot Cell 9:315-24. 2010b>Rev3 polymerase and Mph1 DNA helicase participate in error-prone and error-free pathways, respectively, for the bypassing of template lesions during DNA replication... - Lesion bypass by S. cerevisiae Pol ? aloneJana E Stone
Laboratory of Molecular Genetics and Laboratory of Structural Biology, National Institute of Environmental Health Sciences, NIH, DHHS, Research Triangle Park, NC 27709, USA
DNA Repair (Amst) 10:826-34. 2011..The results are consistent with the hypothesis that, in addition to extending aberrant termini created by other DNA polymerases, Pol ? has the potential to be the sole DNA polymerase involved in TLS... - Exo1 competes with repair synthesis, converts NER intermediates to long ssDNA gaps, and promotes checkpoint activationMichele Giannattasio
Dipartimento di Scienze Biomolecolari e Biotecnologie, Universita degli Studi di Milano, Milano, 20133, Italy
Mol Cell 40:50-62. 2010..Our work has significant implications for understanding the coordination between repair of DNA lesions and checkpoint pathways to preserve genome stability... - Loss of H3 K79 trimethylation leads to suppression of Rtt107-dependent DNA damage sensitivity through the translesion synthesis pathwayNancy Lévesque
Department of Medical Genetics, University of British Columbia, Centre for Molecular Medicine and Therapeutics, Child and Family Research Institute, Vancouver, British Columbia V5Z 4H4, Canada
J Biol Chem 285:35113-22. 2010..These data revealed a multifaceted functional relationship between Rtt107 and Dot1 in the DNA damage response and maintenance of genome integrity... - Two Mms2 residues cooperatively interact with ubiquitin and are critical for Lys63 polyubiquitination in vitro and in vivoLandon Pastushok
Department of Microbiology and Immunology, University of Saskatchewan, Saskatoon, SK, Canada
FEBS Lett 581:5343-8. 2007..Taken together, this study identifies key residues of the Mms2-Ub interface and provides direct experimental evidence that Mms2 physical association with Ub is correlated with its ability to promote Lys63-linked Ub chain assembly... - Post-replication repair suppresses duplication-mediated genome instabilityChristopher D Putnam
Ludwig Institute for Cancer Research, University of California San Diego School of Medicine, La Jolla, California, United States of America
PLoS Genet 6:e1000933. 2010..Our analysis is consistent with models in which PRR prevents replication damage from becoming double strand breaks (DSBs) and/or regulates the activity of HR on DSBs... - Low-fidelity DNA synthesis by the L979F mutator derivative of Saccharomyces cerevisiae DNA polymerase zetaJana E Stone
Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences Research, NIH, DHHS, Research Triangle Park, NC 27709, USA
Nucleic Acids Res 37:3774-87. 2009..Saccharomyces cerevisiae Pol zeta in which phenyalanine was substituted for the conserved Leu-979 in the catalytic (Rev3) subunit... - Abasic sites in the transcribed strand of yeast DNA are removed by transcription-coupled nucleotide excision repairNayun Kim
Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 2771, USA
Mol Cell Biol 30:3206-15. 2010..Such transcription-coupled NER of AP sites may explain previously suggested links between the BER pathway and transcription... - 3-Methyl-3-deazaadenine, a stable isostere of N3-methyl-adenine, is efficiently bypassed by replication in vivo and by transcription in vitroPaola Monti
Molecular Mutagenesis and DNA Repair Unit, Department of Epidemiology and Prevention, National Cancer Research Institute IST, 16132 Genova, Italy
DNA Repair (Amst) 10:861-8. 2011..We conclude that, in these experimental systems, 3-m-c(3)A is efficiently bypassed by replication in vivo and by transcription in vitro... - Error-free RAD52 pathway and error-prone REV3 pathway determines spontaneous mutagenesis in Saccharomyces cerevisiaeKingo Endo
Graduate School of Life Sciences, Tohoku University, Sendai, Japan
Genes Genet Syst 82:35-42. 2007..gene in haploid cells or heterozygous diploid cells, we characterized the effects of mutations in the RAD52 and REV3 genes of Saccharomyces cerevisiae in spontaneous mutagenesis... - Effects of hexavalent chromium on the survival and cell cycle distribution of DNA repair-deficient S. cerevisiaeTravis J O'Brien
Molecular and Cellular Oncology Program, Department of Pharmacology, The George Washington University Medical Center, Washington, DC 20037, USA
DNA Repair (Amst) 1:617-27. 2002..Wild-type, translesion synthesis (rev3) and excision repair (apn1, ntg1, ntg2, rad1) mutants exhibited similar survival following Cr(VI) treatment (0-50mM).. - S. cerevisiae has three pathways for DNA interstrand crosslink repairK F Grossmann
Department of Molecular and Medical Genetics, Oregon Health Sciences University, Mail Code L103, 3181 SW Sam Jackson Park Road, Portland, OR 97201, USA
Mutat Res 487:73-83. 2001Yeast mutants, snm1 (pso2-1), rev3 (pso1-1), and rad51, which display significant sensitivity to interstrand crosslinks (ICLs) have low relative sensitivity to other DNA damaging agents... - Phosphorylation of Rad55 on serines 2, 8, and 14 is required for efficient homologous recombination in the recovery of stalled replication forksKristina Herzberg
Section of Microbiology, University of California, Davis, Davis, CA 95616 8665, USA
Mol Cell Biol 26:8396-409. 2006..These results suggest that Rad55-S2,8,14 phosphorylation activates recombinational repair, allowing for faster recovery after genotoxic stress... - Genomic screening in vivo reveals the role played by vacuolar H+ ATPase and cytosolic acidification in sensitivity to DNA-damaging agents such as cisplatinChunyan Liao
Pharmaceutical Science Research Division, King s College London, Franklin Wilkins Building, 150 Stamford Street, London SE1 9NH, UK
Mol Pharmacol 71:416-25. 2007..The increased loss in cell viability induced by cisplatin at lower pH in V-ATPase mutants supports this hypothesis. The loss in viability seen in wild-type cells under the same conditions was far less dramatic... - CDC7/DBF4 functions in the translesion synthesis branch of the RAD6 epistasis group in Saccharomyces cerevisiaeLuis Pessoa-Brandao
Molecular Biology Program, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA
Genetics 167:1597-610. 2004..Several genes have been determined to function in separate branches within this group, including RAD5, REV3/REV7 (Pol zeta), RAD30 (Pol eta), and POL30 (PCNA)... - Saccharomyces cerevisiae Sae2- and Tel1-dependent single-strand DNA formation at DNA break promotes microhomology-mediated end joiningKihoon Lee
Department of Molecular Medicine and Institute of Biotechnology, University of Texas Health Science Center at San Antonio, 15355 Lambda Drive, San Antonio, TX 78245, USA
Genetics 176:2003-14. 2007..3' flap removal by Rad1/Rad10, Nej1, and DNA synthesis by multiple polymerases including Pol4, Rad30, Rev3, and Pol32. The mismatch repair proteins, Rad52 group genes, and Rad27 are dispensable for MMEJ... - Delineating the requirements for spontaneous DNA damage resistance pathways in genome maintenance and viability in Saccharomyces cerevisiaeNatalie J Morey
Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322, USA
Genetics 164:443-55. 2003..In diploids, the presence of PRR alone may confer a lethal mutation load or, alternatively, PRR alone may be insufficient to deal with potentially lethal, replication-blocking lesions... - Saccharomyces cerevisiae polymerase zeta functions in mitochondriaHengshan Zhang
Department of Cancer Genetics, Roswell Park Cancer Institute, Buffalo, New York 14263, USA
Genetics 172:2683-8. 2006..Supporting this evidence, both polymerase zeta and Rev1p were found to be localized in the mitochondria. This is the first report demonstrating that the DNA polymerase zeta and Rev1 proteins function in the mitochondria... - The S. cerevisiae Mag1 3-methyladenine DNA glycosylase modulates susceptibility to homologous recombinationC A Hendricks
Division of Bioengineering and Environmental Health, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
DNA Repair (Amst) 1:645-59. 2002..Disruption of REV3 (required for polymerase zeta (Pol zeta)) in mag1 rad4 cells causes increased susceptibility to methylation-induced .. - Interactions between mutations for sensitivity to psoralen photoaddition (pso) and to radiation (rad) in Saccharomyces cerevisiaeJ A Henriques
J Bacteriol 148:248-56. 1981..photoaddition, which induces both deoxyribonucleic acid interstrand cross-links and monoadditions, the pso1 mutation is epistatic to the rad6, rad52, and pso2 mutations, whereas it is synergistic to rad3... - A mutation in EXO1 defines separable roles in DNA mismatch repair and post-replication repairPhuoc T Tran
Department of Radiation Oncology, Stanford Hospital and Clinics, Stanford, CA 94305, USA
DNA Repair (Amst) 6:1572-83. 2007..Lastly, by using a compound exo1 mutant that was defective for interaction with Mlh1p and deficient for nuclease activity, we provide further evidence that Exo1p plays both structural and catalytic roles during MMR... - Interactions of Exo1p with components of MutLalpha in Saccharomyces cerevisiaeP T Tran
Department of Molecular and Medical Genetics, Oregon Health Sciences University, Portland 97201, USA
Proc Natl Acad Sci U S A 98:9760-5. 2001..Finally, our results show that much of the increase in spontaneous mutation observed in an exo1Delta strain is REV3-dependent, in turn suggesting that Exo1p is also involved in one or more MMR-independent mutation avoidance pathways... - Overlapping specificities of base excision repair, nucleotide excision repair, recombination, and translesion synthesis pathways for DNA base damage in Saccharomyces cerevisiaeR L Swanson
Departments of Biochemistry, Department of Radiation Oncology, Emory University School of Medicine, Atlanta, Georgia 30322, USA
Mol Cell Biol 19:2929-35. 1999..cerevisiae. In addition, the fact that these responses to induced and spontaneous damage depend upon the simultaneous loss of Ntg1p, Ntg2p, and Apn1p suggests a physiological role for the AP lyase activity of Ntg1p and Ntg2p in vivo... - Eukaryotic DNA polymerases in DNA replication and DNA repairP M Burgers
Washington University School of Medicine, Department of Biochemistry and Molecular Biophysics, St Louis, MO 63110, USA
Chromosoma 107:218-27. 1998..The role of DNA polymerase beta in base-excision repair is well established for mammalian systems, but in yeast, DNA polymerase delta appears to fulfill that function... - Mutagenesis of benzo[a]pyrene diol epoxide in yeast: requirement for DNA polymerase zeta and involvement of DNA polymerase etaZhongwen Xie
Graduate Center for Toxicology, University of Kentucky, Lexington, Kentucky 40536, USA
Biochemistry 42:11253-62. 2003..In rev3 mutant cells lacking Pol(zeta), (+/-)-anti-BPDE-induced mutagenesis was mostly abolished, leading to a great .. - Translesion synthesis of acetylaminofluorene-dG adducts by DNA polymerase zeta is stimulated by yeast Rev1 proteinDongyu Guo
Graduate Center for Toxicology, University of Kentucky, Lexington, KY 40536, USA
Nucleic Acids Res 32:1122-30. 2004..Consistent with the in vitro results, both Polzeta and Rev1 were found to be equally important for error-prone translesion synthesis across from AAF-dG DNA adducts in yeast cells... - Phosphorylation of Slx4 by Mec1 and Tel1 regulates the single-strand annealing mode of DNA repair in budding yeastSonja Flott
MRC Protein Phosphorylation Unit, James Black Centre, University of Dundee, Dundee DD1 5EH, United Kingdom
Mol Cell Biol 27:6433-45. 2007..These results indicate that Slx4 has multiple functions in responding to DNA damage and that a subset of these are regulated by Mec1/Tel1-dependent phosphorylation... - ATR homolog Mec1 controls association of DNA polymerase zeta-Rev1 complex with regions near a double-strand breakYukinori Hirano
Department of Cell Biology and Molecular Medicine, University of Medicine and Dentistry of New Jersey, New Jersey Medical School, Newark, New Jersey 07103, USA
Curr Biol 16:586-90. 2006..Polzeta consists of two subunits, one encoded by REV3 (the catalytic subunit) and the other encoded by REV7... - Rev1 and Polzeta influence toxicity and mutagenicity of Me-lex, a sequence selective N3-adenine methylating agentPaola Monti
Department of Epidemiology and Prevention, National Cancer Research Institute IST, L go R Benzi, 10, 16132 Genova, Italy
DNA Repair (Amst) 7:431-8. 2008..the contribution of translesion synthesis (TLS) DNA polymerases in the bypass of Me-lex-induced lesions, the REV3 and REV1 genes were independently deleted in the parental yeast strain and in different DNA repair-deficient .. - Analysis of the spontaneous mutator phenotype associated with 20S proteasome deficiency in S. cerevisiaeJustyna McIntyre
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02 106 Warsaw, Poland
Mutat Res 593:153-63. 2006..Taken together, our findings strongly support the idea that proteolytic activity is involved in modulating the balance between TLS mechanisms functioning during DNA replication in S. cerevisiae... - Evaluation of the roles of Pol zeta and NHEJ in starvation-associated spontaneous mutagenesis in the yeast Saccharomyces cerevisiaeAgnieszka Halas
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland
Curr Genet 55:245-51. 2009..in starvation-associated spontaneous base substitutions and frameshifts, using yeast mutants carrying deletions of REV3 (encoding the catalytic subunit of Pol zeta), YKU80 (encoding a protein involved in the initiation of NHEJ), or .. - Requirement of HSM3 gene for spontaneous mutagenesis in Saccharomyces cerevisiaeI V Fedorova
Laboratory of Eucaryote Genetics, Division of Molecular and Radiation Biophysics, Petersburg Nuclear Physics Institute, RAS, Orlova Roscha, Gatchina 188350, Leningrad District, Russia
Mutat Res 554:67-75. 2004..mutants was observed in slowly dividing cells in the rad1, rad2, rad14, rad54, and pms1, but it was absent in the rev3, pol2 and pol3 mutants... - Yeast MPH1 gene functions in an error-free DNA damage bypass pathway that requires genes from Homologous recombination, but not from postreplicative repairK Anke Schürer
Department of Molecular Genetics and Preparative Molecular Biology, Institute for Microbiology and Genetics, University of Gottingen, D 37077 Gottingen, Germany
Genetics 166:1673-86. 2004..The dependence of the mph1 mutator phenotype on REV3 and REV1 and the synergy with mutations in base and nucleotide excision repair suggest an involvement of MPH1 in .. - Eukaryotic Y-family polymerases bypass a 3-methyl-2'-deoxyadenosine analog in vitro and methyl methanesulfonate-induced DNA damage in vivoBrian S Plosky
Laboratory of Genomic Integrity, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 3371, USA
Nucleic Acids Res 36:2152-62. 2008..contributes to the survival of cells exposed to methyl methanesulfonate (MMS) and in the absence of Mag1, Rad30 and Rev3, human polymerases eta, iota and kappa are capable of restoring MMS-resistance to the normally MMS-sensitive strain. - dUTPase activity is critical to maintain genetic stability in Saccharomyces cerevisiaeMarie Guillet
CEA, DSV Département de Radiobiologie et Radiopathologie, UMR 217 CNRS Radiobiologie Moléculaire et Cellulaire, BP 6, 92265 Fontenay aux Roses, France
Nucleic Acids Res 34:2056-66. 2006..the dut1-1 strain mutation spectrum showed that cytosines are preferentially incorporated in front of AP sites in a Rev3-dependent manner during translesion synthesis... - MPH1, a yeast gene encoding a DEAH protein, plays a role in protection of the genome from spontaneous and chemically induced damageJ Scheller
Abteilung Molekulare Genetik und Präparative Molekularbiologie, Institut fur Mikrobiologie und Genetik, Georg August Universitat Gottingen, 37077 Gottingen, Germany
Genetics 155:1069-81. 2000..The mutator phenotype was dependent on REV3 and RAD6. The mutant was sensitive to MMS, EMS, 4-NQO, and camptothecin, but not to UV light and X rays... - Role of Saccharomyces cerevisiae chromatin assembly factor-I in repair of ultraviolet radiation damage in vivoJ C Game
Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA
Genetics 151:485-97. 1999..We find an increased loss of telomeric gene silencing in rad6Delta cac1Delta and rad18Delta cac1Delta double mutants, suggesting that CAF-I and multiple factors in the postreplicative repair pathway influence chromosome structure...
Research Grants
- Etiology of Chromosome TranslocationsSang Eun Lee; Fiscal Year: 2010..This application will identify and characterize damage- surveillance and other mechanisms that suppress chromosome translocation to understand the defects in blood cancers and develop novel preventive and/or therapeutic strategies. ..
- Etiology of Chromosome TranslocationsSang Eun Lee; Fiscal Year: 2009..This application will identify and characterize damage- surveillance and other mechanisms that suppress chromosome translocation to understand the defects in blood cancers and develop novel preventive and/or therapeutic strategies. ..
- Suppression of translocations by RecQ-like DNA helicasesKRISTINA HILDEGARD SCHMIDT; Fiscal Year: 2010....
- Mechanisms of Error Prone Repair of DNA BreaksSang Eun Lee; Fiscal Year: 2010..The information gleaned from the proposal will also provide a logical framework as to how cells tolerate cancer therapeutic agent-induced DNA lesions and shed lights on the prevention and improved therapeutic intervention of cancers. ..
- Mechanisms of Error Prone Repair of DNA BreaksSang Eun Lee; Fiscal Year: 2009..The information gleaned from the proposal will also provide a logical framework as to how cells tolerate cancer therapeutic agent-induced DNA lesions and shed lights on the prevention and improved therapeutic intervention of cancers. ..
- Mechanisms of Error Prone Repair of DNA BreaksSang Eun Lee; Fiscal Year: 2007..Since the components of DSB repair are conserved from yeast to humans, the insights garnered from our research will be valuable for dissecting the equivalent process in human cells and will be of relevance to public health. ..
- Mechanisms of Nonhomologous Repair of Damaged DNASang Eun Lee; Fiscal Year: 2005..Furthermore, since DSB repair by NHEJ is remarkably conserved from yeast to humans, these studies will help to dissect the similar pathways in humans. ..
- Suppression of translocations by RecQ-like DNA helicasesKRISTINA HILDEGARD SCHMIDT; Fiscal Year: 2010....