Research Topics
Genomes and GenesSpecies | MSL5SummaryGene Symbol: MSL5 Description: Msl5p Species: Top Publications
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Publications
A dual role for BBP/ScSF1 in nuclear pre-mRNA retention and splicingB Rutz
EMBL, Meyerhofstrasse 1, 69117 Heidelberg, Germany
EMBO J 19:1873-86. 2000The MSL5 gene, which codes for the splicing factor BBP/ScSF1, is essential in Saccharomyces cerevisiae, yet previous analyses failed to reveal a defect in assembly of (pre)-spliceosomes or in vitro splicing associated with its depletion...
Transient interaction of BBP/ScSF1 and Mud2 with the splicing machinery affects the kinetics of spliceosome assemblyB Rutz
European Molecular Biology Laboratory, Heidelberg, Germany
RNA 5:819-31. 1999..These observations support a model in which BBP and Mud2 are recycled during transition from CC2 to pre-spliceosome...
Mer1p is a modular splicing factor whose function depends on the conserved U2 snRNP protein Snu17pMarc Spingola
Center for the Molecular Biology of RNA, Sinsheimer Laboratories, University of California, Santa Cruz, Santa Cruz, CA 95064, USA
Nucleic Acids Res 32:1242-50. 2004..We conclude that Mer1p is a modular splicing regulator that can activate splicing at several early steps of spliceosome assembly and depends on the activities of both U1 and U2 snRNP proteins to activate splicing...
Cross-intron bridging interactions in the yeast commitment complex are conserved in mammalsN Abovich
Howard Hughes Medical Institute, Department of Biology, Brandeis University, Waltham, Massachusetts 02254, USA
Cell 89:403-12. 1997..Our results show that SF1 interacts strongly with human U2AF65, and that SF1 is a bona fide E complex component. This implies that aspects of these novel cross-intron protein-protein interactions are conserved between yeast and mammals...
The splicing factor BBP interacts specifically with the pre-mRNA branchpoint sequence UACUAACJ A Berglund
Howard Hughes Medical Institute, Department of Biology and Biochemistry, Brandeis University, Waltham, Massachusetts 02254, USA
Cell 89:781-7. 1997..The relative conservation of this region in yeast and mammals correlates well with the RNA-binding differences between BBP and mBBP, suggesting that BBP contributes to branchpoint sequence definition in both systems...
Cotranscriptional spliceosome assembly occurs in a stepwise fashion and requires the cap binding complexJanina Görnemann
Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany
Mol Cell 19:53-63. 2005..Thus, the demonstration of an essential link between CBC and spliceosome assembly in vivo indicates that 5' end capping couples pre-mRNA splicing to transcription...
An extended RNA binding site for the yeast branch point-binding protein and the role of its zinc knuckle domains in RNA bindingStephen M Garrey
Department of Chemistry and Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403 1229, USA
J Biol Chem 281:27443-53. 2006..The lack of involvement of the second highly conserved zinc knuckle in RNA binding suggests that this zinc knuckle plays a different role in RNA processing than enhancing the binding of BBP to the BPS...
A BBP-Mud2p heterodimer mediates branchpoint recognition and influences splicing substrate abundance in budding yeastQiang Wang
Department of Biology and Department of Chemistry, University of Kentucky, Lexington, KY 40506 0225, USA
Nucleic Acids Res 36:2787-98. 2008..In addition, our results suggest the possibility that the Mud2p may enhance the turnover of pre-mRNA with impaired BBP-branchpoint association...
Research Grants
- Regulation of mRNA Splicing by Mer1pMarc Spingola; Fiscal Year: 2007..This project will elucidate how Mer1p and similar proteins function and will offer important insights towards the basis of diseases like Fragile X Syndrome in humans. ..
