VAMP2

Summary

Gene Symbol: VAMP2
Description: vesicle-associated membrane protein 2 (synaptobrevin 2)
Alias: SYB2, VAMP-2, synaptobrevin 2, synaptobrevin-2, vesicle associated membrane protein 2, vesicle-associated membrane protein 2
Species: human

Top Publications

  1. ncbi Transport route for synaptobrevin via a novel pathway of insertion into the endoplasmic reticulum membrane
    U Kutay
    Max Delbrück, Centre for Molecular Medicine, Berlin Buch
    EMBO J 14:217-23. 1995
  2. ncbi A splice-isoform of vesicle-associated membrane protein-1 (VAMP-1) contains a mitochondrial targeting signal
    S Isenmann
    Division of Human Immunology, Hanson Centre for Cancer Research, Adelaide 5000, South Australia, Australia
    Mol Biol Cell 9:1649-60. 1998
  3. ncbi Structures and chromosomal localizations of two human genes encoding synaptobrevins 1 and 2
    B T Archer
    Howard Hughes Medical Institute, University of Texas Southwestern Medical School, Dallas 75235
    J Biol Chem 265:17267-73. 1990
  4. ncbi Syntaxin 13 mediates cycling of plasma membrane proteins via tubulovesicular recycling endosomes
    R Prekeris
    Howard Hughes Medical Institute, Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, California 94305 5428, USA
    J Cell Biol 143:957-71. 1998
  5. ncbi Insulin-responsive tissues contain the core complex protein SNAP-25 (synaptosomal-associated protein 25) A and B isoforms in addition to syntaxin 4 and synaptobrevins 1 and 2
    M N Jagadish
    CSIRO, Division of Biomolecular Engineering, Parkville, Victoria, Australia
    Biochem J 317:945-54. 1996
  6. ncbi Direct interaction of the rat unc-13 homologue Munc13-1 with the N terminus of syntaxin
    A Betz
    Max Planck Institut fur experimentelle Medizin, Abteilung Molekulare Neurobiologie, Hermann Rein Strasse 3, D 37075 Gottingen, Federal Republic of Germany
    J Biol Chem 272:2520-6. 1997
  7. ncbi SNAP-25, a t-SNARE which binds to both syntaxin and synaptobrevin via domains that may form coiled coils
    E R Chapman
    Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, Connecticut 06510
    J Biol Chem 269:27427-32. 1994
  8. ncbi SNARE proteins are critical for regulated exocytosis of ECP from human eosinophils
    H J Hoffmann
    Allergy and Lung Research Laboratory, University Hospital of Aarhus, DK-8000 Aarhus C, Denmark
    Biochem Biophys Res Commun 280:172-6. 2001
  9. ncbi SNARE proteins are critical for regulated exocytosis of ECP from human eosinophils
    H J Hoffmann
    Allergy and Lung Research Laboratory, University of Aarhus, Aarhus C, DK-8000, Denmark
    Biochem Biophys Res Commun 282:194-9. 2001
  10. ncbi Activation of protein kinase C zeta induces serine phosphorylation of VAMP2 in the GLUT4 compartment and increases glucose transport in skeletal muscle
    L Braiman
    Faculty of Life Sciences, Gonda-Goldschmied Center, Bar-Ilan University, Ramat-Gan 52900, Israel
    Mol Cell Biol 21:7852-61. 2001

Research Grants

  1. STAGES OF REGULATED EXOCYTOSIS
    Thomas Martin; Fiscal Year: 2009
  2. STAGES OF REGULATED EXOCYTOSIS
    Thomas F J Martin; Fiscal Year: 2010
  3. STAGES OF REGULATED EXOCYTOSIS
    Thomas Martin; Fiscal Year: 2009
  4. MOLECULAR MECHANISMS OF PLATELET EXOCYTOSIS
    Sidney Whiteheart; Fiscal Year: 2007
  5. MOLECULAR MECHANISMS OF PLATELET EXOCYTOSIS
    SIDNEY WALDO WHITEHEART; Fiscal Year: 2010
  6. The function of ATM protein in the biology of the adult neuron CNS:
    Karl Herrup; Fiscal Year: 2010
  7. Vesicle recycling at developing NMJs
    Rita Balice Gordon; Fiscal Year: 2009
  8. Vesicle recycling at developing NMJs
    Rita J Balice Gordon; Fiscal Year: 2010
  9. Mechanisms of fusion and transmitter release in neurosecretion
    Manfred Lindau; Fiscal Year: 2009
  10. Mechanisms of fusion and transmitter release in neurosecretion
    Manfred Lindau; Fiscal Year: 2010

Scientific Experts

Detail Information

Publications161 found, 100 shown here

  1. ncbi Transport route for synaptobrevin via a novel pathway of insertion into the endoplasmic reticulum membrane
    U Kutay
    Max Delbrück, Centre for Molecular Medicine, Berlin Buch
    EMBO J 14:217-23. 1995
    ..Membrane integration requires ER protein(s) different from the translocation components needed for proteins with signal sequences, thus suggesting a novel mechanism of insertion...
  2. ncbi A splice-isoform of vesicle-associated membrane protein-1 (VAMP-1) contains a mitochondrial targeting signal
    S Isenmann
    Division of Human Immunology, Hanson Centre for Cancer Research, Adelaide 5000, South Australia, Australia
    Mol Biol Cell 9:1649-60. 1998
    ..These data suggest that mitochondria may be integrated, at least at a mechanistic level, to the vesicular trafficking pathways that govern protein movement between other organelles of the cell...
  3. ncbi Structures and chromosomal localizations of two human genes encoding synaptobrevins 1 and 2
    B T Archer
    Howard Hughes Medical Institute, University of Texas Southwestern Medical School, Dallas 75235
    J Biol Chem 265:17267-73. 1990
    ..Two cosmid clones containing the human genes encoding synaptobrevins 1 and 2 (gene symbols SYB1 and SYB2, respectively) were isolated and characterized...
  4. ncbi Syntaxin 13 mediates cycling of plasma membrane proteins via tubulovesicular recycling endosomes
    R Prekeris
    Howard Hughes Medical Institute, Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, California 94305 5428, USA
    J Cell Biol 143:957-71. 1998
    ..13 revealed that, in Triton X-100 extracts, syntaxin 13 is present in a complex(es) comprised of betaSNAP, VAMP 2/3, and SNAP-25...
  5. ncbi Insulin-responsive tissues contain the core complex protein SNAP-25 (synaptosomal-associated protein 25) A and B isoforms in addition to syntaxin 4 and synaptobrevins 1 and 2
    M N Jagadish
    CSIRO, Division of Biomolecular Engineering, Parkville, Victoria, Australia
    Biochem J 317:945-54. 1996
    ..Furthermore, we have confirmed the presence of syntaxin 4 in fat and of synaptobrevin 2 in skeletal muscle by PCR amplification and Southern hybridization analysis...
  6. ncbi Direct interaction of the rat unc-13 homologue Munc13-1 with the N terminus of syntaxin
    A Betz
    Max Planck Institut fur experimentelle Medizin, Abteilung Molekulare Neurobiologie, Hermann Rein Strasse 3, D 37075 Gottingen, Federal Republic of Germany
    J Biol Chem 272:2520-6. 1997
    ..It is possible that the phenotype of unc-13 and unc-18 mutations is caused by the inability of the respective mutated gene products to bind to syntaxin...
  7. ncbi SNAP-25, a t-SNARE which binds to both syntaxin and synaptobrevin via domains that may form coiled coils
    E R Chapman
    Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, Connecticut 06510
    J Biol Chem 269:27427-32. 1994
    ..We conclude that SNAP-25 can interact with both syntaxin and synaptobrevin and that binding may be mediated by alpha-helical domains that form intermolecular coiled-coil structures...
  8. ncbi SNARE proteins are critical for regulated exocytosis of ECP from human eosinophils
    H J Hoffmann
    Allergy and Lung Research Laboratory, University Hospital of Aarhus, DK-8000 Aarhus C, Denmark
    Biochem Biophys Res Commun 280:172-6. 2001
    ..Thus, these results strongly suggest a critical role of SNAREs in regulated exocytosis in eosinophils...
  9. ncbi SNARE proteins are critical for regulated exocytosis of ECP from human eosinophils
    H J Hoffmann
    Allergy and Lung Research Laboratory, University of Aarhus, Aarhus C, DK-8000, Denmark
    Biochem Biophys Res Commun 282:194-9. 2001
    ..Thus, these results strongly suggest a critical role of SNAREs in regulated exocytosis in eosinophils...
  10. ncbi Activation of protein kinase C zeta induces serine phosphorylation of VAMP2 in the GLUT4 compartment and increases glucose transport in skeletal muscle
    L Braiman
    Faculty of Life Sciences, Gonda-Goldschmied Center, Bar-Ilan University, Ramat-Gan 52900, Israel
    Mol Cell Biol 21:7852-61. 2001
    ..These results demonstrate that PKC zeta regulates insulin-stimulated GLUT4 translocation and glucose transport through the unique colocalization of this isoform with the GLUT4 compartments...
  11. ncbi Differential distribution of vesicle associated membrane protein isoforms in the mouse retina
    David M Sherry
    College of Optometry, University of Houston, Houston, TX 77204 2020, USA
    Mol Vis 9:673-88. 2003
    ..Two VAMP isoforms have been identified that are considered to be synaptic, VAMP-1 and VAMP-2, however their distributions among the various synapses in the mammalian retina have not been characterized...
  12. ncbi Expression of two membrane fusion proteins, synaptosome-associated protein of 25 kDa and vesicle-associated membrane protein, in choroid plexus epithelium
    I Chung
    Department of Clinical Neurosciences, Brown University School of Medicine, Aldrich Building, Room 403, 593 Eddy Street, Providence, RI 02903, USA
    Neuroscience 116:349-57. 2003
    ....
  13. ncbi Biochemical and ultrastructural analyses of IgLON cell adhesion molecules, Kilon and OBCAM in the rat brain
    S Miyata
    Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo ku, Kyoto 606 8585, Japan
    Neuroscience 117:645-58. 2003
    ..These differences possibly represent diverse functions of each IgLON molecule...
  14. ncbi Immunohistochemical localization of SNARE proteins in dental pulp and periodontal ligament of the rat incisor
    Shiho Honma
    Department of Oral Anatomy and Developmental Biology, Osaka University Graduate School of Dentistry, Osaka, Japan
    Anat Rec (Hoboken) 293:1070-80. 2010
    ..Because those proteins participate in docking and exocytosis of synapse vesicles in the central nervous system, they might also contribute to vesicle exocytosis at receptive fields where apparent synapses are not present...
  15. ncbi Glut4 storage vesicles without Glut4: transcriptional regulation of insulin-dependent vesicular traffic
    Danielle N Gross
    Department of Biochemistry, Boston University School of Medicine, MA 02118, USA
    Mol Cell Biol 24:7151-62. 2004
    ..They also express components of the insulin-sensitive vesicular transport machinery, namely, VAMP2, syntaxin-4, and IRAP, the last of these being the other marker of insulin-regulated vesicular traffic along with ..
  16. ncbi The glucose transporter (GLUT-4) and vesicle-associated membrane protein-2 (VAMP-2) are segregated from recycling endosomes in insulin-sensitive cells
    S Martin
    Centre for Molecular and Cellular Biology, University of Queensland, St Lucia, Brisbane, Australia
    J Cell Biol 134:625-35. 1996
    ..In addition, GLUT-4 and VAMP-2 are selectively enriched in a postendocytic compartment. Further study is required to elucidate the function of this latter compartment in insulin-responsive cells...
  17. ncbi Abnormality of synaptic vesicular associated proteins in cerebral cortex and hippocampus after microwave exposure
    LiFeng Wang
    Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, People s Republic of China
    Synapse 63:1010-6. 2009
    ..The perturbation could induce the deposit of synaptic vesicle, which might be relative to the dysfunction of the synaptic transmission, even the cognition deficit...
  18. ncbi Stimulation of GLUT4 (glucose transporter isoform 4) storage vesicle formation by sphingolipid depletion
    Zhi jie Cheng
    Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
    Biochem J 427:143-50. 2010
    ..Glycosphingolipid depletion also enhanced the insulin-induced translocation of VAMP2 (vesicle-associated membrane protein 2), but not the transferrin receptor or cellubrevin, indicating that the ..
  19. ncbi S100A10-mediated translocation of annexin-A2 to SNARE proteins in adrenergic chromaffin cells undergoing exocytosis
    Emeline Umbrecht-Jenck
    Institut des Neurosciences Cellulaires et Integratives, UPR 3212 CNRS, Universite de Strasbourg, 5 rue Blaise Pascal, F 67084 Strasbourg, France
    Traffic 11:958-71. 2010
    ..Here, we demonstrate that S100A10 can interact with vesicle-associated membrane protein 2 (VAMP2) and show that VAMP2 is present at the plasma membrane in resting adrenergic chromaffin cells...
  20. ncbi cAMP increases surface expression of NKCC2 in rat thick ascending limbs: role of VAMP
    Pablo A Ortiz
    Hypertension and Vascular Research Division, Dept of Internal Medicine, Henry Ford Hospital, 2799 W Grand Blvd, Detroit, MI 48202, USA
    Am J Physiol Renal Physiol 290:F608-16. 2006
    ..min(-1).mm(-1); not significant). We concluded that cAMP increases NKCC2 surface expression by a mechanism involving VAMP and that NKCC2 trafficking to the apical membrane is involved in the stimulation of TAL NaCl absorption by cAMP...
  21. ncbi Vesicle-associated membrane protein-2/synaptobrevin binding to synaptotagmin I promotes O-glycosylation of synaptotagmin I
    Mitsunori Fukuda
    Fukuda Initiative Research Unit, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Biol Chem 277:30351-8. 2002
    ....
  22. ncbi Syntaxin isoform specificity in the regulation of renal H+-ATPase exocytosis
    Guangmu Li
    Renal Section, Boston University Medical Center and Department of Medicine, Boston University School of Medicine, Massachusetts 02118 2908, USA
    J Biol Chem 278:19791-7. 2003
    ..Interaction between the syntaxin-1A and H+-ATPase is important in the targeted exocytosis of the proton pump to the apical membrane of intercalated cells...
  23. ncbi Immuno-localization of vesicular acetylcholine transporter in mouse taste cells and adjacent nerve fibers: indication of acetylcholine release
    Tatsuya Ogura
    Department of Biological Sciences, University of Maryland Baltimore County, Baltimore, MD 21250, USA
    Cell Tissue Res 330:17-28. 2007
    ..Thus, functions of taste receptor cells could be modulated by trigeminal fibers via ACh release as well...
  24. ncbi SNAP-25/syntaxin 1A complex functionally modulates neurotransmitter gamma-aminobutyric acid reuptake
    Hua Ping Fan
    Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
    J Biol Chem 281:28174-84. 2006
    ..Thus our findings delineate a further molecular mechanism for the regulation of GABA reuptake by a target SNARE complex and suggest a direct coordination between GABA release and reuptake...
  25. ncbi Characterization of Munc-18c and syntaxin-4 in 3T3-L1 adipocytes. Putative role in insulin-dependent movement of GLUT-4
    J T Tellam
    Centre for Molecular and Cellular Biology and Department of Physiology and Pharmacology, University of Queensland, St Lucia 4072, Queensland, Australia
    J Biol Chem 272:6179-86. 1997
    ..These data suggest that syntaxin-4/Munc-18c/VAMP-2 may play a role in the docking/fusion of intracellular GLUT-4-containing vesicles with the cell surface in adipocytes...
  26. ncbi The C2B domain of rabphilin directly interacts with SNAP-25 and regulates the docking step of dense core vesicle exocytosis in PC12 cells
    Takashi Tsuboi
    Fukuda Initiative Research Unit, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Biol Chem 280:39253-9. 2005
    ..These findings suggest that rabphilin is involved in the docking step of regulated exocytosis in PC12 cells, possibly through interaction between the C2B domain and SNAP-25...
  27. ncbi Vesicle-associated membrane protein isoforms in the tiger salamander retina
    D M Sherry
    College of Optometry, University of Houston, Houston, Texas 77004, USA
    J Comp Neurol 431:424-36. 2001
    ..These results also corroborate previous studies in Xenopus indicating that the N-terminus of nonmammalian VAMP isoforms differs from their mammalian counterparts...
  28. ncbi Association of N-ethylmaleimide sensitive fusion (NSF) protein and soluble NSF attachment proteins-alpha and -gamma with glucose transporter-4-containing vesicles in primary rat adipocytes
    C C Mastick
    Department of Cell Biology, Parke Davis Pharmaceutical Research Division, Warner Lambert Company, Ann Arbor, Michigan 48105, USA
    Endocrinology 138:2391-7. 1997
    ..These data suggest that the steady state association of NSF and the SNAPs with GLUT4 vesicles and cell membranes is independent of the formation of fusion complexes...
  29. ncbi Gangliosides as high affinity receptors for tetanus neurotoxin
    Chen Chen
    Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    J Biol Chem 284:26569-77. 2009
    ..These data show that gangliosides are functional dual receptors for TeNT...
  30. ncbi A protein chip membrane-capture assay for botulinum neurotoxin activity
    Séverine Marconi
    Institut National de la Sante et de la Recherche Medicale, Unité 641, Marseille F 13916, France
    Toxicol Appl Pharmacol 233:439-46. 2008
    ..agents which inhibit neurotransmission by cleaving the intra-cellular presynaptic SNARE proteins SNAP-25 and VAMP2, localized respectively in plasma membrane and synaptic vesicles...
  31. ncbi Transient silencing of synaptic transmitter release from specific neuronal types by recombinant tetanus toxin light chain fused to antibody variable region
    Tomoko Kobayashi
    Department of Molecular Genetics, Institute of Biomedical Sciences, Fukushima Medical University School of Medicine, Fukushima, Japan
    J Neurosci Methods 175:125-32. 2008
    ..Our ITet technology provides a useful approach for inducible and reversible control of synaptic transmission in specific neuronal types in the brain...
  32. ncbi Pantophysin is a phosphoprotein component of adipocyte transport vesicles and associates with GLUT4-containing vesicles
    C C Brooks
    Research Division, Joslin Diabetes Center and Department of Medicine, Harvard Medical School, Boston, Massachusetts 02215, USA
    J Biol Chem 275:2029-36. 2000
    ..In addition, pantophysin may provide a marker for the analysis of other vesicles in adipocytes...
  33. ncbi Synaptotagmin VI and VIII and syntaxin 2 are essential for the mouse sperm acrosome reaction
    Darren M Hutt
    Ottawa Health Research Institute, Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, Ontario K1Y 4E9, Canada
    J Biol Chem 280:20197-203. 2005
    ..N-ethylmaleimide-sensitive factor attachment protein receptor) complex, composed of syntaxin (Stx), SNAP-25, and VAMP2, mediates vesicle fusion, whereas calcium regulation is thought to be accomplished by the synaptotagmin (Syt) ..
  34. ncbi Qualitative and quantitative differences between taste buds of the rat and mouse
    Huazhi Ma
    Department of Biological Sciences, University of Denver, Denver, Colorado 80210, USA
    BMC Neurosci 8:5. 2007
    ..5 and synaptobrevin-2 to determine the percentages of taste cells expressing these markers in taste buds in both rodent species. We also determined the numbers of taste cells in the taste buds as well as taste bud volume...
  35. ncbi Diabetes differentially affects the content of exocytotic proteins in hippocampal and retinal nerve terminals
    J M Gaspar
    Center of Ophthalmology and Vision Sciences, IBILI, Faculty of Medicine, University of Coimbra, 3004 548 Coimbra, Portugal
    Neuroscience 169:1589-600. 2010
    ..Since diabetes differentially affects exocytotic proteins, according to tissue and insult duration, functional studies will be required to assess the physiological impairment induced by diabetes on the exocytosis in central synapses...
  36. ncbi A tetanus toxin sensitive protein other than VAMP 2 is required for exocytosis in the pancreatic acinar cell
    P J Padfield
    Department of Pathology, Saint Louis University Medical Centre, South Grand Boulevard, St Louis, MO 63104, USA
    FEBS Lett 484:129-32. 2000
    ..BoNT/D) had no detectable effect on Ca(2+)-dependent amylase secretion but did result in the complete cleavage of VAMP 2. In comparison, tetanus toxin (TeTx) treatment both significantly inhibited Ca(2+)-dependent amylase secretion and ..
  37. ncbi A proteomic survey of rat cerebral cortical synaptosomes
    Frank A Witzmann
    Department of Cellular and Integrative Physiology, Indiana University School of Medicine, Indianapolis, 46202, USA
    Proteomics 5:2177-201. 2005
    ..The results also suggest that this experimental approach can be used to study the differential expression of multiple proteins involved in alterations of synaptic function...
  38. ncbi Vesicle-associated membrane protein 2 plays a specific role in the insulin-dependent trafficking of the facilitative glucose transporter GLUT4 in 3T3-L1 adipocytes
    L B Martin
    Centre for Molecular and Cellular Biology, University of Queensland, St Lucia, Australia
    J Biol Chem 273:1444-52. 1998
    Vesicle-associated membrane protein 2 (VAMP2) has been implicated in the insulin-regulated trafficking of GLUT4 in adipocytes. It has been proposed that VAMP2 co-localizes with GLUT4 in a postendocytic storage compartment (Martin, S...
  39. ncbi Disturbances of soluble N-ethylmaleimide-sensitive factor attachment proteins in hippocampal synaptosomes contribute to cognitive impairment after repetitive formaldehyde inhalation in male rats
    Y Liu
    Neuroscience Research Institute and Department of Neurobiology, Key Laboratory for Neuroscience, Ministry of Education, Ministry of Public Health, Peking University Health Science Center, Beijing 100191, PR China
    Neuroscience 169:1248-54. 2010
    ..composed of syntaxin1 and synaptosome-associated protein 25 (SNAP25) on the plasma membrane and synaptobrevin/VAMP2 (vesicle-associated membrane protein 2) on the vesicle membrane, plays a key role in synaptic exocytosis and ..
  40. ncbi Increased plasma membrane localization of O-glycosylation-deficient mutant of synaptotagmin I in PC12 cells
    Eiko Kanno
    Laboratory of Membrane Trafficking Mechanisms, Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences, Tohoku University, Aobayama, Aoba ku, Sendai, Miyagi, Japan
    J Neurosci Res 86:1036-43. 2008
    ..The results indicate that O-glycosylation, not N-glycosylation, is partially involved in efficient targeting of Syt I protein to dense-core vesicles in PC12 cells...
  41. ncbi Immunocytochemical evidence that rod-connected horizontal cell axon terminals remodel in response to experimental retinal detachment in the cat
    Kenneth A Linberg
    Neuroscience Research Institute, University of California, Santa Barbara, CA 93106, USA
    Mol Vis 12:1674-86. 2006
    ..We have previously described neurite sprouting from HCs in response to experimental retinal detachment. Here we sought to determine whether one or both types elaborate these outgrowths...
  42. ncbi Dehydroepiandrosterone induces a neuroendocrine phenotype in nerve growth factor-stimulated chromaffin pheochromocytoma PC12 cells
    Christian G Ziegler
    University Hospital Carl Gustav Carus, Medical Clinic III, University of Dresden, 01307 Dresden, Germany
    Endocrinology 149:320-8. 2008
    ..DHEA drives NGF-stimulated cells toward a neuroendocrine phenotype, suggesting that the interaction of intraadrenal steroids and growth factors is required for the maintenance of an intact adrenal medulla...
  43. ncbi Identification of soluble N-ethylmaleimide-sensitive factor attachment protein receptor exocytotic machinery in human plasma cells: SNAP-23 is essential for antibody secretion
    Elena Reales
    Unidad de Investigacion, Hospital Universitario Puerta del Mar, Cadiz, Spain
    J Immunol 175:6686-93. 2005
    ..These results provide insights, for the first time, into the molecular machinery of constitutive vesicular trafficking in human PC Ig secretion and present evidence indicating that at least SNAP-23 is essential for Ab production...
  44. ncbi Gene expression related to synaptogenesis, neuritogenesis, and MAP kinase in behavioral sensitization to psychostimulants
    Hiroshi Ujike
    Department of Neuropsychiatry, Okayama University Medical School and Graduate School of Medicine and Dentistry, Okayama, Japan
    Ann N Y Acad Sci 965:55-67. 2002
    ..Synaptophysin mRNA, but not VAMP2 (synaptobrevin 2) mRNA, which are considered as synaptogenesis markers, increased in the accumbens, striatum, hippocampus, and ..
  45. ncbi Rab3a and SNARE proteins: potential regulators of melanosome movement
    G Scott
    Department of Dermatology, Box 697, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA
    J Invest Dermatol 116:296-304. 2001
    ....
  46. ncbi Molecular substrates mediating lanthanide-evoked neurotransmitter release in central synapses
    ChiHye Chung
    Department of Neuroscience, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390 9111, USA
    J Neurophysiol 100:2089-100. 2008
    ..For the delayed action, La3+ may act intracellularly after its entry or through intracellular Ca2+ via a mechanism that does not require synaptobrevin-2...
  47. ncbi Insulin recruits GLUT4 from distinct compartments via distinct traffic pathways with differential microtubule dependence in rat adipocytes
    Li bin Liu
    Department of Cell Biology, Institute for Molecular and Cellular Regulation, Gunma University, 3 39 15 Showa Machi, Maebashi 371 8512, Japan
    J Biol Chem 278:30157-69. 2003
    ..These data suggest that insulin recruits GLUT4 to the plasma membrane from at least two distinct intracellular compartments via distinct traffic routes with differential microtubule dependence in rat primary adipocytes...
  48. ncbi The role of the t-SNARE SNAP-25 in action potential-dependent calcium signaling and expression in GABAergic and glutamatergic neurons
    Lawrence C R Tafoya
    Department of Neurosciences, University of New Mexico Health Sciences Center, Albuquerque, USA
    BMC Neurosci 9:105. 2008
    ..In this study, we investigate the effects of SNAP-25 ablation on spontaneous neuronal activity and the expression of functionally distinct isoforms of this t-SNARE in GABAergic and glutamatergic neurons of the adult brain...
  49. ncbi Transmembrane-domain determinants for SNARE-mediated membrane fusion
    Elena Fdez
    Institute of Parasitology and Biomedicine López Neyra, Consejo Superior de Investigaciones Cientificas, Avda del Conocimiento s n, 18100 Granada, Spain
    J Cell Sci 123:2473-80. 2010
    ..fusion is mediated by the SNARE complex, which is composed of the vesicle-associated protein synaptobrevin (VAMP2), and the plasma membrane proteins syntaxin-1A and SNAP-25...
  50. ncbi Synaptobrevin 2 is palmitoylated in synaptic vesicles prepared from adult, but not from embryonic brain
    M Veit
    Department of Immunology and Molecular Biology, Free University, Berlin, Germany
    Mol Cell Neurosci 15:408-16. 2000
    ..Here we have analyzed palmitoylation of the SNARE-proteins synaptobrevin 2 and synaptotagmin in vitro using synaptosomal and synaptic vesicle preparations from rat brain...
  51. ncbi Subchronic alpha-linolenic acid treatment enhances brain plasticity and exerts an antidepressant effect: a versatile potential therapy for stroke
    Nicolas Blondeau
    Cerebrovascular Pathologies and Therapeutic Laboratory, Institut de Pharmacologie Moléculaires et Cellulaires UMR6097, C N R S, Valbonne, France
    Neuropsychopharmacology 34:2548-59. 2009
    ....
  52. ncbi Visual Arrestin 1 acts as a modulator for N-ethylmaleimide-sensitive factor in the photoreceptor synapse
    Shun Ping Huang
    Mary D Allen Laboratory for Vision Research, Doheny Eye Institute, Department of Ophthalmology, Keck School of Medicine, University of Southern California, Los Angeles, California 90033 9224, USA
    J Neurosci 30:9381-91. 2010
    ..components, including vGLUT1 (vesicular glutamate transporter 1), EAAT5 (excitatory amino acid transporter 5), and VAMP2 (vesicle-associated membrane protein 2), are markedly reduced, which leads to a substantial decrease in the ..
  53. ncbi Role of SNARE's in the GLUT4 translocation response to insulin in adipose cells and muscle
    J F St-Denis
    Experimental Diabetes, Metabolism, and Nutrition Section, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Basic Clin Physiol Pharmacol 9:153-65. 1998
    ..Several v- and t-SNARE's have been identified in adipose cells and muscle. VAMP2 and VAMP3/cellubrevin (v-SNARE's) have been shown to interact with the t-SNARE's syntaxin 4 and SNAP-23...
  54. ncbi Different presynaptic roles of synapsins at excitatory and inhibitory synapses
    Daniel Gitler
    Department of Neurobiology, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Neurosci 24:11368-80. 2004
    ..by measuring synaptic vesicle density in living neurons transfected with green fluorescent protein-tagged synaptobrevin 2, a marker of synaptic vesicles...
  55. ncbi ECL cell morphology
    D Chen
    Department of Pharmacology, University of Lund, Sweden
    Yale J Biol Med 71:217-31. 1998
    ..Subsequent lysosomal degradation of the vacuoles and their contents resulted in the development of lipofuscin bodies...
  56. ncbi Ca2+ dynamics in the secretory vesicles of neurosecretory PC12 and INS1 cells
    Jaime Santodomingo
    Instituto de Biologia y Genetica Molecular, Departamento de Bioquimica y Biologia Molecular y Fisiologia, Facultad de Medicina, Universidad de Valladolid and Consejo Superior de Investigaciones Cientificas, Ramon y Cajal, 7, 47005 Valladolid, Spain
    Cell Mol Neurobiol 30:1267-74. 2010
    ....
  57. ncbi Three-dimensional structure of the amino-terminal domain of syntaxin 6, a SNAP-25 C homolog
    Kira M S Misura
    Departments of Structural Biology and Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA 94305
    Proc Natl Acad Sci U S A 99:9184-9. 2002
    ..SNARE motif sequences fall into four classes, homologous to the neuronal proteins syntaxin 1a, VAMP 2, and the N- and C-terminal SNARE motifs of SNAP-25 (S25N and S25C), and it is thought that one member from each ..
  58. ncbi Molecular mechanisms of substrate recognition and specificity of botulinum neurotoxin serotype F
    Sheng Chen
    Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong
    Biochem J 433:277-84. 2011
    ..The understanding of the molecular mechanisms of LC/F substrate recognition provides insights into the development of antitoxins and engineering novel BoNTs to optimize current therapy and extend therapeutic interventions...
  59. ncbi Molecular basis for tetanus toxin coreceptor interactions
    Chen Chen
    Microbiology and Molecular Genetics, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, Wisconsin 53226, USA
    Biochemistry 47:7179-86. 2008
    ..This analysis provides a model for how tetanus toxin utilizes coreceptors for high-affinity binding to neurons...
  60. ncbi Intravesicular calcium release mediates the motion and exocytosis of secretory organelles: a study with adrenal chromaffin cells
    Marcial Camacho
    Unidad de Farmacologia, Facultad de Medicina, Universidad de La Laguna Tenerife, E 38071 La Laguna, Spain
    J Biol Chem 283:22383-9. 2008
    ..fluorescence microscopy in cells overexpressing the enhanced green fluorescent protein-labeled synaptobrevin (VAMP2-EGFP) protein. The disruption of the vesicular gradient of pH caused the leak of Ca2+, measured with fura-2...
  61. ncbi Combinatorial SNARE complexes modulate the secretion of cytoplasmic granules in human neutrophils
    Faustino Mollinedo
    Centro de Investigacion del Cancer, Instituto de Biologia Molecular y Celular del Cancer, Consejo Superior de Investigaciones Cientificas Universidad de Salamanca, Salamanca, Spain
    J Immunol 177:2831-41. 2006
    ....
  62. ncbi Ca(2+)-independent syntaxin binding to the C(2)B effector region of synaptotagmin
    Toshio Masumoto
    Department of Physiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2 5 1 Shikata cho, Kita ku, Okayama Shi, Okayama 700 8558, Japan
    Mol Cell Neurosci 49:1-8. 2012
    ..SNARE complexes comprising syntaxin 1, 25-kDa synaptosomal-associated protein (SNAP-25), and synaptobrevin 2 were coprecipitated with synaptotagmin I in the presence of ethylene glycol tetraacetic acid...
  63. ncbi Characterization of immunoisolated human gastric parietal cells tubulovesicles: identification of regulators of apical recycling
    Lynne A Lapierre
    Dept of Surgery, Vanderbilt Univ School of Medicine, 4160A MRB III, 465 21st St S, Nashville, TN 37232 2733, USA
    Am J Physiol Gastrointest Liver Physiol 292:G1249-62. 2007
    ..compartment, whereas a less dramatic effect was observed in cells transfected with syntaxin 3, syntaxin 7, or VAMP2. The five DsRed2-SNARE chimeras were also transfected into a MDCK cell line overexpressing Rab11-FIP2(129-512)...
  64. ncbi Identification of the amino acid residues rendering TI-VAMP insensitive toward botulinum neurotoxin B
    Stefan Sikorra
    Institut fur Biochemie, OE 4310 Medizinische Hochschule Hannover, D 30623 Hannover, Germany
    J Mol Biol 357:574-82. 2006
    ..However, the insensitivity of TI-VAMP to botulinum neurotoxin B relies on at least 12 amino acid changes versus VAMP-2. These are scattered along an interface of 22 amino acid residues in length...
  65. ncbi VAMP2-dependent exocytosis regulates plasma membrane insertion of TRPC3 channels and contributes to agonist-stimulated Ca2+ influx
    Brij B Singh
    Secretory Physiology Section, Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, Bethesda, MD 20892, USA
    Mol Cell 15:635-46. 2004
    ..Here we demonstrate that TRPC3-N terminus interacts with VAMP2 and alphaSNAP...
  66. ncbi Insulin but not PDGF relies on actin remodeling and on VAMP2 for GLUT4 translocation in myoblasts
    Dora Torok
    Programme in Cell Biology, The Hospital for Sick Children, 555 University Avenue, Toronto, ON, M5G 1X8, Canada
    J Cell Sci 117:5447-55. 2004
    ..via a mechanism dependent on phosphatidylinositol (PtdIns) 3-kinase, actin cytoskeletal remodeling and the v-SNARE VAMP2. The growth factor PDGF-BB also robustly activates PtdIns 3-kinase and induces actin remodeling, raising the ..
  67. ncbi Evidence for a role of the exocyst in insulin-stimulated Glut4 trafficking in 3T3-L1 adipocytes
    Marie Ann Ewart
    Henry Wellcome Laboratory of Cell Biology, Division of Biochemistry and Molecular Biology, Davidson Building, Faculty of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, Scotland, United Kingdom
    J Biol Chem 280:3812-6. 2005
    ..vesicles at the plasma membrane is known to involve the target SNAREs syntaxin 4 and SNAP-23 and the vesicle SNARE VAMP2. Little is known about the initial docking of Glut4 vesicles with the plasma membrane...
  68. ncbi Detection of botulinum toxins: micromechanical and fluorescence-based sensors
    Vladimir Parpura
    Department of Cell Biology and Neuroscience, 1208 Spieth Hall, University of California, Riverside, CA 92521, USA
    Croat Med J 46:491-7. 2005
    ..attachment of a bead to the micromachined cantilever through the interactions between SNARE proteins, with synaptobrevin 2 deposited onto beads and syntaxin 1A deposited onto cantilevers...
  69. ncbi A role for soluble N-ethylmaleimide-sensitive factor attachment protein receptor complex dimerization during neurosecretion
    Elena Fdez
    Institute of Parasitology and Biomedicine López Neyra, Consejo Superior de Investigaciones Cientificas, 18100 Granada, Spain
    Mol Biol Cell 19:3379-89. 2008
    ..of cytosolic SNARE complexes are in apposition, and it involves residues from the vesicle-associated SNARE synaptobrevin 2 that lie close to the cytosol-membrane interface within the full-length protein...
  70. ncbi Differential expression of synaptic proteins after chronic restraint stress in rat prefrontal cortex and hippocampus
    Heidi Kaastrup Müller
    Centre for Psychiatric Research, Aarhus University Hospital, Risskov, Denmark
    Brain Res 1385:26-37. 2011
    ..on the SNARE complex, we investigated the expression of the SNARE core components syntaxin 1A, SNAP-25, and VAMP2 at both transcriptional and protein levels...
  71. ncbi Different substrate recognition requirements for cleavage of synaptobrevin-2 by Clostridium baratii and Clostridium botulinum type F neurotoxins
    Suzanne R Kalb
    Centers for Disease Control and Prevention, National Center for Environmental Health, Division of Laboratory Sciences, 4770 Buford Hwy N E, Atlanta, GA 30341, USA
    Appl Environ Microbiol 77:1301-8. 2011
    ..This peptide substrate is an N-terminal extension of the original peptide substrate used for detection of other BoNT/F toxins and can be used to detect four of the currently known BoNT/F subtypes by mass spectrometry...
  72. ncbi Transcriptome analysis of frontal cortex in alcohol-preferring and nonpreferring rats
    Travis J Worst
    Center for the Neurobehavioral Study of Alcohol, Department of Physiology and Pharmacology, Wake Forest University Health Sciences, Winston Salem, North Carolina, USA
    J Neurosci Res 80:529-38. 2005
    ..ANA lines. In contrast, calcium channel subunit alpha2delta1 (cacna2d1), vesicle-associated membrane protein 2 (VAMP2), syntaxin 1 (both syntaxin 1a and 1b; STX1a and STX1b), and syntaxin binding protein (MUNC-18) mRNAs were found ..
  73. ncbi Granule exocytosis contributes to priming and activation of the human neutrophil respiratory burst
    Silvia M Uriarte
    Department of Medicine, University of Louisville, Louisville, KY 40202, USA
    J Immunol 187:391-400. 2011
    ....
  74. ncbi VAMP-1, VAMP-2, and syntaxin-4 regulate ANP release from cardiac myocytes
    Marcella Ferlito
    Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    J Mol Cell Cardiol 49:791-800. 2010
    ..Our data suggest that three specific SNAREs regulate cardiac myocyte exocytosis of ANP. Pathways that modify the exocytic machinery may influence natriuresis and blood pressure...
  75. ncbi Myosin Va controls oligodendrocyte morphogenesis and myelination
    Jacob A Sloane
    Department of Neurology, Beth Israel Deaconess Medical Center, Harvard Institutes of Medicine, Boston, Massachusetts 02215, USA
    J Neurosci 27:11366-75. 2007
    ..Loss of myosin Va function also blocked distal localization of vesicle-associated membrane protein 2 (VAMP2), which is known to associate with myosin Va...
  76. ncbi Quantifying neurite growth mediated by interactions among secretory vesicles, microtubules, and actin networks
    Krasimira Tsaneva-Atanasova
    Department of Computational Biology, Paris, France
    Biophys J 96:840-57. 2009
    ..To validate one aspect of our model, we demonstrate that the experimental flux of TI-VAMP but not Synaptobrevin 2 vesicles contributes to the neurite growth...
  77. ncbi Is assembly of the SNARE complex enough to fuel membrane fusion?
    Katrin Wiederhold
    Research Group Structural Biochemistry, Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, Gottingen, Germany
    J Biol Chem 284:13143-52. 2009
    ..vesicles are the SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) proteins synaptobrevin 2, syntaxin 1a, and SNAP-25...
  78. ncbi VAMP2 is expressed in myogenic cells during rat development
    Yuki Tajika
    Department of Anatomy, Gunma University Graduate School of Medicine, Gunma, Japan
    Dev Dyn 237:1886-92. 2008
    Vesicle-associated membrane protein 2 (VAMP2) is a member of the SNARE family of proteins that regulate the intracellular vesicle fusion process. This study investigated the developmental expression of VAMP2 in the rat embryo...
  79. ncbi A selective filter for cytoplasmic transport at the axon initial segment
    Ai Hong Song
    Institute of Neuroscience, State Key Laboratory of Neurobiology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai, China
    Cell 136:1148-60. 2009
    ..Axonal entry was allowed for KIF5-driven carriers of synaptic vesicle protein VAMP2, but not for KIF17-driven carriers of dendrite-targeting NMDA receptor subunit NR2B...
  80. ncbi Diabetes downregulates presynaptic proteins and reduces basal synapsin I phosphorylation in rat retina
    Heather D VanGuilder
    The Penn State Retina Research Group, Ulerich Ophthalmology Research Laboratory, Department of Ophthalmology, Penn State College of Medicine, Milton S Hershey Medical Center, 500 University Drive, Hershey, PA 17033, USA
    Eur J Neurosci 28:1-11. 2008
    ....
  81. ncbi A role for the CHC22 clathrin heavy-chain isoform in human glucose metabolism
    Stéphane Vassilopoulos
    Department of Bioengineering and Therapeutic Sciences, University of California, School of Pharmacy, San Francisco UCSF, San Francisco, CA 94143, USA
    Science 324:1192-6. 2009
    ..Thus, CHC22-dependent membrane trafficking constitutes a species-restricted pathway in human muscle and fat with potential implications for type 2 diabetes...
  82. ncbi Palmitoylation of the synaptic vesicle fusion machinery
    Gerald R Prescott
    Centre for Integrative Physiology, School of Biomedical Sciences, University of Edinburgh, Edinburgh, United Kingdom
    J Neurochem 110:1135-49. 2009
    ..1 and SNAP25, which are associated with the pre-synaptic plasma membrane and vesicle-associated membrane protein (VAMP2), a synaptic vesicle SNARE...
  83. ncbi De novo STXBP1 mutations in mental retardation and nonsyndromic epilepsy
    Fadi F Hamdan
    Centre of Excellence in Neuromics of Université de Montréal and Synapse to Disease Group, Centre Hospitalier Universitaire Sainte Justine Research Center, Universite de Montreal, Montreal, Quebec, Canada
    Ann Neurol 65:748-53. 2009
    We sequenced genes coding for components of the SNARE complex (STX1A, VAMP2, SNAP25) and their regulatory proteins (STXBP1/Munc18-1, SYT1), which are essential for neurotransmission, in 95 patients with idiopathic mental retardation...
  84. ncbi An in vitro assay for detection of tetanus neurotoxin activity: using antibodies for recognizing the proteolytically generated cleavage product
    B Kegel
    Paul Ehrlich Institut, Paul Ehrlich Strasse 51 59, 63225 Langen, Germany
    Toxicol In Vitro 21:1641-9. 2007
    ..In the future, the assay may also serve as a basis for the replacement of the in vivo safety control of tetanus vaccines...
  85. ncbi The biological activity of ubiquitinated BoNT/B light chain in vitro and in human SHSY-5Y neuronal cells
    Xuerong Shi
    Department of Regulated Laboratories, Division of Regulated Activities, Walter Reed Army Institute of Research, 503 Robert Grant Avenue, Silver Spring, Maryland 20910 7500, USA
    J Cell Biochem 108:660-7. 2009
    ..Ubiquitination in vitro and in cells decreased the biological activity of BoNT/B light chain. These results further elucidate BoNT protein degradation pathways in intoxicated neuronal cells and mechanisms to enhance toxin removal...
  86. ncbi Evaluation of the heterogeneous reactivity of the syntaxin molecules on the inner leaflet of the plasma membrane
    Dana Bar-On
    Department of Neurobiochemistry, Laser Laboratory for Fast Reactions in Biology, George S Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel
    J Neurosci 29:12292-301. 2009
    ..formation between syntaxin 1A and SNAP-25 (synaptosome-associated protein of 25 kDa) on the PM and synaptobrevin 2 on the vesicles (trans-SNAREs) is crucial for vesicle priming and fusion...
  87. ncbi Heterogeneity of glutamatergic and GABAergic release machinery in cerebral cortex: analysis of synaptogyrin, vesicle-associated membrane protein, and syntaxin
    L Bragina
    Department of Neuroscience, Universita Politecnica delle Marche, Ancona, Italy
    Neuroscience 165:934-43. 2010
    ..80% of VGAT+ puncta; VAMP1 was expressed in approximately 45% of VGLUT1+, 55% of VGLUT2+, and 80% of VGAT+ puncta; VAMP2 in about 95% of VGLUT1+, 75% of VGLUT2+, and 80% of VGAT+ puncta; STX1A in about 65% of VGLUT1+, 30% of VGLUT2+, ..
  88. ncbi Acute sublethal global hypoxia induces transient increase of GAP-43 immunoreactivity in the striatum of neonatal rats
    Susana R Valdez
    IMBECU CRICYT, Centro Regional de Investigaciones Cientificas y Tecnologicas, Mendoza, Argentina
    Synapse 61:124-37. 2007
    ..Modifications in the activity of these proteins could contribute to the long term altered responses to stress due to acute hypoxic insult in the neonatal period...
  89. ncbi A fast mode of membrane fusion dependent on tight SNARE zippering
    Marine Bretou
    Institut de Biologie Physico Chimique, Centre National de la Recherche Scientifique, UPR 1929, Universite Paris, 7 Denis Diderot, 75005 Paris, France
    J Neurosci 28:8470-6. 2008
    ..In contrast, a short intermembrane distance mediated by tight zippering of SNAREs is essential to a component of the secretory response elicited by robust stimuli and characterized by rapid dilation of the fusion pore...
  90. ncbi Soluble NSF attachment protein receptors (SNAREs) in RBL-2H3 mast cells: functional role of syntaxin 4 in exocytosis and identification of a vesicle-associated membrane protein 8-containing secretory compartment
    F Paumet
    Unite d Immuno Allergie, Institut Pasteur, Paris, France
    J Immunol 164:5850-7. 2000
    ..Four VAMP proteins, i.e., VAMP2, cellubrevin, TI-VAMP, and VAMP8, were present on intracellular membrane structures, with VAMP8 residing mainly on ..
  91. ncbi Calmodulin and lipid binding to synaptobrevin regulates calcium-dependent exocytosis
    Stephanie Quetglas
    Institut National de la Santé et de la Recherche Médicale Unité 464, Universite de la Mediterranee, 13916 Marseille Cedex 20, France
    EMBO J 21:3970-9. 2002
    ..release involves the assembly of a heterotrimeric SNARE complex composed of the vesicle protein synaptobrevin (VAMP 2) and two plasma membrane partners, syntaxin 1 and SNAP-25...
  92. ncbi Nerve growth factor-dependent sorting of synaptotagmin IV protein to mature dense-core vesicles that undergo calcium-dependent exocytosis in PC12 cells
    Mitsunori Fukuda
    Fukuda Initiative Research Unit, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Biol Chem 278:3220-6. 2003
    ..Our results suggest that Syt IV is a stimulus (e.g. NGF)-dependent regulator for exocytosis of dense-core vesicles...
  93. ncbi Trafficking of green fluorescent protein-tagged SNARE proteins in HSY cells
    Taishin Takuma
    Department of Oral Biochemistry, School of Dentistry, Health Sciences University of Hokkaido, Tobetsu, Hokkaido 061 0293
    J Biochem 132:729-35. 2002
    ..These results suggest that munc18c plays an important role in the trafficking of syntaxin-4 to its proper destination by preventing premature interactions with other proteins, including SNARE proteins...
  94. ncbi Tetanus and botulinum-B neurotoxins block neurotransmitter release by proteolytic cleavage of synaptobrevin
    G Schiavo
    Dipartimento di Scienze Biomediche, Universita di Padova, Italy
    Nature 359:832-5. 1992
    ..These results indicate that tetanus and botulinum B neurotoxins block neurotransmitter release by cleaving synaptobrevin-2, a protein that, on the basis of our results, seems to play a key part in neurotransmitter release...
  95. ncbi Molecular cloning and characterization of mammalian homologues of vesicle-associated membrane protein-associated (VAMP-associated) proteins
    Y Nishimura
    Department of Molecular and Cellular Pharmacology, Mie University School of Medicine, 2 174 Edobashi, Mie, Tsu, 514 8507, Japan
    Biochem Biophys Res Commun 254:21-6. 1999
    ..hVAP-B had VAMP-binding ability. Moreover, hVAP-A and hVAP-B associated with each other through their respective TMDs. These results suggest that complex formation by VAPs might be important in the trafficking of mammalian vesicle...
  96. ncbi The hypophysis controls expression of SNAP-25 and other SNAREs in the adrenal gland
    R Hepp
    INSERM Unité 338, Biologie de la Communication Cellulaire, 5 rue Blaise Pascal, 67084 Strasbourg Cedex, France
    J Neurocytol 30:789-800. 2001
    ..They suggest that glucocorticoids are unlikely to be directly responsible for this but provide evidence that thyroid hormones are implicated in this phenomenon. The putative role of hormonal regulation on SNARE function is discussed...
  97. ncbi Expression of eosinophil target SNAREs as potential cognate receptors for vesicle-associated membrane protein-2 in exocytosis
    Michael R Logan
    Pulmonary Research Group, Department of Medicine, University of Alberta, Edmonton, Canada
    J Allergy Clin Immunol 109:299-306. 2002
    ....
  98. ncbi A conformational switch in syntaxin during exocytosis: role of munc18
    I Dulubova
    Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75235, USA
    EMBO J 18:4372-82. 1999
    ..Our data suggest a model whereby, during exocytosis, syntaxin undergoes a large conformational switch that mediates the transition between the syntaxin-munc18-1 complex and the core complex...
  99. ncbi Buforin I, a natural peptide, inhibits botulinum neurotoxin B activity in vitro
    G E Garcia
    Division of Biochemistry Walter Reed Army Institute of Research, Washington, DC 20307, USA
    J Appl Toxicol 19:S19-22. 1999
    ..cleaves between the amino acids glutamine and phenylalanine (Q and F bond) in position 76-77 of synaptobrevin (VAMP2)...
  100. ncbi Identification of the endoplasmic reticulum targeting signal in vesicle-associated membrane proteins
    P K Kim
    Department of Biochemistry, McMaster University, Hamilton, Ontario L8N 3Z5, Canada
    J Biol Chem 274:36876-82. 1999
    ..Unlike other insertion sequence-containing proteins, targeting of the highly homologous Vamp1 and Vamp2 to the endoplasmic reticulum requires ATP and a membrane-bound receptor...
  101. ncbi SNARE proteins are highly enriched in lipid rafts in PC12 cells: implications for the spatial control of exocytosis
    L H Chamberlain
    Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, Davidson Building, University of Glasgow, Glasgow G12 8QQ, United Kingdom
    Proc Natl Acad Sci U S A 98:5619-24. 2001
    ..However, further analysis revealed that the majority of VAMP2 was associated with a distinct class of raft with different detergent solubility characteristics to the rafts ..

Research Grants63

  1. STAGES OF REGULATED EXOCYTOSIS
    Thomas Martin; Fiscal Year: 2009
    ..Because mutations in these proteins result in human disease (e.g., in immune system function), the work will contribute to understanding the underlying basis of these pathologies. ..
  2. STAGES OF REGULATED EXOCYTOSIS
    Thomas F J Martin; Fiscal Year: 2010
    ..Because mutations in these proteins result in human disease (e.g., in immune system function), the work will contribute to understanding the underlying basis of these pathologies. ..
  3. STAGES OF REGULATED EXOCYTOSIS
    Thomas Martin; Fiscal Year: 2009
    ..Because mutations in these proteins result in human disease (e.g., in immune system function), the work will contribute to understanding the underlying basis of these pathologies. ..
  4. MOLECULAR MECHANISMS OF PLATELET EXOCYTOSIS
    Sidney Whiteheart; Fiscal Year: 2007
    ....
  5. MOLECULAR MECHANISMS OF PLATELET EXOCYTOSIS
    SIDNEY WALDO WHITEHEART; Fiscal Year: 2010
    ....
  6. The function of ATM protein in the biology of the adult neuron CNS:
    Karl Herrup; Fiscal Year: 2010
    ..as a chaperone protein that nucleates a complex of proteins that includes vesicle proteins such as synapsin-I and VAMP2. We propose that it is the failure of the functions associated with cytoplasmic ATM that leads to many of the ..
  7. Vesicle recycling at developing NMJs
    Rita Balice Gordon; Fiscal Year: 2009
    ..SpH is a pH-sensitive variant of GFP tethered to the luminal domain of the vesicular protein VAMP2 that allows synaptic vesicle recycling to be monitored optically...
  8. Vesicle recycling at developing NMJs
    Rita J Balice Gordon; Fiscal Year: 2010
    ..SpH is a pH-sensitive variant of GFP tethered to the luminal domain of the vesicular protein VAMP2 that allows synaptic vesicle recycling to be monitored optically...
  9. Mechanisms of fusion and transmitter release in neurosecretion
    Manfred Lindau; Fiscal Year: 2009
    ..Molecular manipulations of the SNARE proteins SNAP-25 and VAMP2 are performed in conjunction with measurements of fusion pore opening, fusion pore conductance, lifetime, ..
  10. Mechanisms of fusion and transmitter release in neurosecretion
    Manfred Lindau; Fiscal Year: 2010
    ..Molecular manipulations of the SNARE proteins SNAP-25 and VAMP2 are performed in conjunction with measurements of fusion pore opening, fusion pore conductance, lifetime, ..
  11. Functional mapping of SNARE-dependent trafficking and fusion
    Jeffrey E Pessin; Fiscal Year: 2010
    ..of biophysical and cell biological approaches to determine the role of membrane electrostatics and the tandem VAMP2 tryptophans in this key biological process...
  12. Mechanisms of Bacterial Toxin Action
    Joseph T Barbieri; Fiscal Year: 2010
    ..characterize the intracellular trafficking of TeNT in neurons and determine how TeNT cleaves the SNARE protein, VAMP2. These studies define mechanistic properties of toxins that may lead to translational products for the detection, ..
  13. Role of SNARE Interactions in Central Synapse Function
    Ege T Kavalali; Fiscal Year: 2010
    Synaptobrevin-2/VAMP-2 (syb2) is an abundant synaptic vesicle protein essential for normal synaptic transmission in the brain...
  14. AFM studies of SNARE-mediated membrane fusion
    Vincent T Moy; Fiscal Year: 2010
    ..Trans pairing of the neuronal v-SNARE (VAMP2) and t-SNAREs (syntaxin and SNAP-25) has been shown to form the essential molecular machinery necessary to induce ..
  15. AFM studies of SNARE-mediated membrane fusion
    Vincent T Moy; Fiscal Year: 2010
    ..Trans pairing of the neuronal v-SNARE (VAMP2) and t-SNAREs (syntaxin and SNAP-25) has been shown to form the essential molecular machinery necessary to induce ..
  16. AFM studies of SNARE-mediated membrane fusion
    Vincent T Moy; Fiscal Year: 2010
    ..Trans pairing of the neuronal v-SNARE (VAMP2) and t-SNAREs (syntaxin and SNAP-25) has been shown to form the essential molecular machinery necessary to induce ..
  17. CHARACTERIZATION AND SORTING OF ZYMOGEN GRANULE PROTEINS
    ANSON LOWE; Fiscal Year: 2000
    ..The role of protein processing in GP2 sorting will also be studied. Characterization of GP2 processing will lead to a better understanding of its function, sorting, and potential role in human diseases such as chronic pancreatitis. ..
  18. Regulation of GLUT4 Exocytosis
    Jingshi Shen; Fiscal Year: 2007
    ..The translocation involves a vesicle fusion step that is mediated by three SNAREs - syntaxin 4, SNAP-23 and VAMP2 - and a number of regulatory proteins including Munc18c, synip and tomosyn...
  19. Lacrimal acinar secretory vesicle biogenesis and release
    Ronald Marchelletta; Fiscal Year: 2006
    Rab 3D- and VAMP2-enriched vesicles represent two potentially distinct populations of secretory vesicles in primary rabbit lacrimal acini...
  20. INTRACELLULAR TRAFFICKING OF GLUT4 CONTAINING VESICLES
    Jeffrey Pessin; Fiscal Year: 2004
    ..determining the interaction of Synip with the other established syntaxin 4 binding proteins (Munc18c, SNAP23 and VAMP2) in regulation insulin- stimulated GLUT4 translocation...
  21. HORMONE REGULATION OF (CA++)I IN PANCREATIC ACINAR CELLS
    Shmuel Muallem; Fiscal Year: 2003
    ..4. Prob cellular microdomains with targeted cameleons. ER-cameleon and cameleons fused to the N and C termini of VAMP2 and Syncollin will be packaged into adenoviruses and infected into acinar cells...
  22. MECHANISM OF OXIDANT-INDUCED RESPIRATORY MUCIN SECRETION
    KENNETH ADLER; Fiscal Year: 2007
    ..translocation to the plasma membrane, fusion and docking proteins associated with the secretory module (Rab 3, VAMP 2 and VAMP 8) in conjunction with target proteins in the plasmlemma, position the granules for exocytosis...
  23. Identifying molecules that augment mutant CFTR activity
    Anjaparavanda Naren; Fiscal Year: 2006
    ..Finally, these studies should help us identify a few novel cell permeant organic molecules that can augment CFTR activity of partial loss of function CFTR mutants that will eventually be useful in CF therapy. ..
  24. Role of SNARE Interactions in Central Synapse Function
    EGE KAVALALI; Fiscal Year: 2007
    Synaptobrevin2/VAMP2 (syb2) is a synaptic vesicle associated protein critical for normal synaptic transmission in central synapses...