Skp2

Summary

Gene Symbol: Skp2
Description: S-phase kinase-associated protein 2 (p45)
Alias: FBL1, FBXL1, FLB1, CDK2/cyclin A-associated protein p45, F-box/LRR-repeat protein 1, S-phase kinase-associated protein 2, p45skp2
Species: human

Top Publications

  1. ncbi Relation of overexpression of S phase kinase-associated protein 2 with reduced expression of p27 and PTEN in human gastric carcinoma
    Xiu Mei Ma
    Fourth Affiliated Hospital of Hebei Medical University, Shijiazhuang 050011, Hebei Province, China
    World J Gastroenterol 11:6716-21. 2005
  2. ncbi Suppression of anoikis by SKP2 amplification and overexpression promotes metastasis of esophageal squamous cell carcinoma
    Xiao Chun Wang
    State Key Laboratory of Molecular Oncology, Cancer Institute Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, P O Box 2258, Beijing 100021, China
    Mol Cancer Res 7:12-22. 2009
  3. ncbi The tumor suppressor ING3 is degraded by SCF(Skp2)-mediated ubiquitin-proteasome system
    G Chen
    Department of Dermatology and Skin Science, Jack Bell Research Centre, Vancouver Coastal Health Research Institute, University of British Columbia, Vancouver, British Columbia, Canada V6H 3Z6
    Oncogene 29:1498-508. 2010
  4. ncbi Skp2 and p27kip1 expression in melanocytic nevi and melanoma: an inverse relationship
    Qing Li
    Department of Pathology and Laboratory Medicine, Albany Medical College, New Scotland Ave, Albany, NY 12208, USA
    J Cutan Pathol 31:633-42. 2004
  5. ncbi Mechanism of beta 1-integrin-mediated hepatoma cell growth involves p27 and S-phase kinase-associated protein 2
    Hao Zhang
    Division of Hepatology and Metabolism, Department of Internal Medicine, Saga Medical School, Nabeshima, Saga, Japan
    Hepatology 38:305-13. 2003
  6. ncbi Expression of Skp2 and p27KIP1 in naevi and malignant melanoma of the skin and its relation to clinical outcome
    C Woenckhaus
    Institute of Pathology, University of Greifswald, Greifswald, Germany
    Histol Histopathol 20:501-8. 2005
  7. ncbi Promotion of NEDD-CUL1 conjugate cleavage by COP9 signalosome
    S Lyapina
    Department of Biology, Howard Hughes Medical Institute, California Institute of Technology, Pasadena, CA 91125, USA
    Science 292:1382-5. 2001
  8. ncbi Significance of skp2 expression in primary breast cancer
    Hideto Sonoda
    Department of Surgery, Kyushu University Hospital at Beppu, Beppu, Japan
    Clin Cancer Res 12:1215-20. 2006
  9. ncbi The pRb-related protein p130 is regulated by phosphorylation-dependent proteolysis via the protein-ubiquitin ligase SCF(Skp2)
    Donato Tedesco
    Department of Molecular Biology, MB 7, The Scripps Research Institute, La Jolla, California 92037, USA
    Genes Dev 16:2946-57. 2002
  10. ncbi p19Skp1 and p45Skp2 are essential elements of the cyclin A-CDK2 S phase kinase
    H Zhang
    Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, New York 11724, USA
    Cell 82:915-25. 1995

Research Grants

  1. F-box proteins: deregulated cell cycle control and proteolysis in cancer
    Michele Pagano; Fiscal Year: 2007
  2. B-RAF Regulation of the Cell Cycle in Melanoma
    Andrew E Aplin; Fiscal Year: 2010
  3. Cell Cycle Regulation and Adult T Cell Leukemia
    Chou Zen Giam; Fiscal Year: 2010
  4. HTLV-I Tax activates the anaphase promoting complex
    Chou Zen Giam; Fiscal Year: 2010
  5. ECM MATRIX, PROTEASE AND MELANOMA PROLIFERATION
    Yves DeClerck; Fiscal Year: 2007
  6. Physiologic Regulation of Hematopoiesis by Notch
    Nadia Carlesso; Fiscal Year: 2010
  7. Physiologic Regulation of Hematopoiesis by Notch
    Nadia Carlesso; Fiscal Year: 2009
  8. Regulation of Bladder Smooth Muscle Hyperplasia
    Paul Austin; Fiscal Year: 2007
  9. Regulation of the G1 Phase by the Ubiquitin Pathway
    Michele Pagano; Fiscal Year: 2005
  10. Transformation of human mammary epithelium by c-Myc
    Robert Dickson; Fiscal Year: 2005

Detail Information

Publications161 found, 100 shown here

  1. ncbi Relation of overexpression of S phase kinase-associated protein 2 with reduced expression of p27 and PTEN in human gastric carcinoma
    Xiu Mei Ma
    Fourth Affiliated Hospital of Hebei Medical University, Shijiazhuang 050011, Hebei Province, China
    World J Gastroenterol 11:6716-21. 2005
    To investigate the significance of S phase kinase-associated protein 2 (Skp2) expression in human gastric carcinoma and the relation between expressions of Skp2, p27 and PTEN.
  2. ncbi Suppression of anoikis by SKP2 amplification and overexpression promotes metastasis of esophageal squamous cell carcinoma
    Xiao Chun Wang
    State Key Laboratory of Molecular Oncology, Cancer Institute Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, P O Box 2258, Beijing 100021, China
    Mol Cancer Res 7:12-22. 2009
    The gene of SKP2, located on chromosome 5p13, plays a critical role in cell cycle progression, especially at the G(1)-S transition, putatively through its control of several cell cycle regulator proteins including p27(kip1), p21(cip1), ..
  3. ncbi The tumor suppressor ING3 is degraded by SCF(Skp2)-mediated ubiquitin-proteasome system
    G Chen
    Department of Dermatology and Skin Science, Jack Bell Research Centre, Vancouver Coastal Health Research Institute, University of British Columbia, Vancouver, British Columbia, Canada V6H 3Z6
    Oncogene 29:1498-508. 2010
    ..Knockdown of F-box protein S-phase kinase-associated protein 2 (Skp2) reduces the ubiquitination of ING3 and significantly stabilizes ING3 in melanoma cells...
  4. ncbi Skp2 and p27kip1 expression in melanocytic nevi and melanoma: an inverse relationship
    Qing Li
    Department of Pathology and Laboratory Medicine, Albany Medical College, New Scotland Ave, Albany, NY 12208, USA
    J Cutan Pathol 31:633-42. 2004
    S-phase kinase associated protein-2 (Skp2) ubiquitin ligase p45(SKP2) is important in the degradation of p27kip1 (a cyclin dependent kinase inhibitor) and progression through the G1-S cell-cycle checkpoint...
  5. ncbi Mechanism of beta 1-integrin-mediated hepatoma cell growth involves p27 and S-phase kinase-associated protein 2
    Hao Zhang
    Division of Hepatology and Metabolism, Department of Internal Medicine, Saga Medical School, Nabeshima, Saga, Japan
    Hepatology 38:305-13. 2003
    ..proliferation and expression of cyclin-dependent kinase (Cdk) inhibitors and S-phase kinase-associated protein 2 (Skp2), were investigated. HGF dose-dependently suppressed the proliferation of parental and mock-transfected HepG2 cells...
  6. ncbi Expression of Skp2 and p27KIP1 in naevi and malignant melanoma of the skin and its relation to clinical outcome
    C Woenckhaus
    Institute of Pathology, University of Greifswald, Greifswald, Germany
    Histol Histopathol 20:501-8. 2005
    b>Skp2 (S-phase kinase associated protein 2) controls progression from G- to S-phase by promoting the proteolysis of the cyclin dependent kinase inhibitor p27KIP1...
  7. ncbi Promotion of NEDD-CUL1 conjugate cleavage by COP9 signalosome
    S Lyapina
    Department of Biology, Howard Hughes Medical Institute, California Institute of Technology, Pasadena, CA 91125, USA
    Science 292:1382-5. 2001
    ....
  8. ncbi Significance of skp2 expression in primary breast cancer
    Hideto Sonoda
    Department of Surgery, Kyushu University Hospital at Beppu, Beppu, Japan
    Clin Cancer Res 12:1215-20. 2006
    ..the p27 expression level to be an independent prognostic factor, and a high S-phase kinase-associated protein 2 (Skp2) expression level was significantly correlated with a poor prognosis in patients with gastric cancer...
  9. ncbi The pRb-related protein p130 is regulated by phosphorylation-dependent proteolysis via the protein-ubiquitin ligase SCF(Skp2)
    Donato Tedesco
    Department of Molecular Biology, MB 7, The Scripps Research Institute, La Jolla, California 92037, USA
    Genes Dev 16:2946-57. 2002
    ..The activity of the ubiquitin ligase complex Skp1-Cul1/Cdc53-F-box protein Skp2 (SCF(Skp2)) and the proteasome were necessary for p130 degradation...
  10. ncbi p19Skp1 and p45Skp2 are essential elements of the cyclin A-CDK2 S phase kinase
    H Zhang
    Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, New York 11724, USA
    Cell 82:915-25. 1995
    ..The abundance of p45 is greatly increased in many transformed cells. This could result in changes in cell cycle control that contribute to the process of cellular transformation...
  11. ncbi Phosphorylation-dependent regulation of cytosolic localization and oncogenic function of Skp2 by Akt/PKB
    Hui Kuan Lin
    Department of Pathology, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nat Cell Biol 11:420-32. 2009
    b>Skp2 is an F-box protein that forms the SCF complex with Skp1 and Cullin-1 to constitute an E3 ligase for ubiquitylation...
  12. ncbi p107 inhibits G1 to S phase progression by down-regulating expression of the F-box protein Skp2
    Geneviève Rodier
    Institut de recherche en immunovirologie et cancérologie, Universite de Montreal, Montreal, Quebec H3C 3J7, Canada
    J Cell Biol 168:55-66. 2005
    ..Instead, we observed a marked decrease in the abundance of the F-box receptor Skp2 in p107-overexpressing cells. Reciprocally, Skp2 accumulates to higher levels in p107-/- embryonic fibroblasts...
  13. ncbi SKP2 associates with p130 and accelerates p130 ubiquitylation and degradation in human cells
    Sabyasachi Bhattacharya
    Fels Institute for Cancer Research and Molecular Biology and Department of Biochemistry, Temple University School of Medicine, 3307 North Broad Street, Philadelphia, PA 19140, USA
    Oncogene 22:2443-51. 2003
    ..Since the SKP1/Cul1/SKP2 E3 ubiquitin ligase complex mediates ubiquitylation of substrates previously phosphorylated by cyclin-dependent ..
  14. ncbi An Rb-Skp2-p27 pathway mediates acute cell cycle inhibition by Rb and is retained in a partial-penetrance Rb mutant
    Peng Ji
    Department of Developmental and Molecular Biology, The Albert Einstein Comprehensive Cancer Center, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Mol Cell 16:47-58. 2004
    ..Conversely, Rb knockout leads to faster p27 degradation. Rb interacts with the N terminus of Skp2, interferes with Skp2-p27 interaction, and inhibits ubiquitination of p27...
  15. ncbi Amplification and overexpression of SKP2 are associated with metastasis of non-small-cell lung cancers to lymph nodes
    Sana Yokoi
    Department of Molecular Cytogenetics, Graduate School of Biomedical Science, Medical Research Institute, Tokyo Medical and Dental University, 1 5 45 Yushima, Bunkyo ku, Tokyo 113 8519, Japan
    Am J Pathol 165:175-80. 2004
    b>SKP2, an F-box protein constituting the substrate recognition subunit of the SCF(SKP2) ubiquitin ligase complex, is implicated in ubiquitin-mediated degradation of the cyclin-dependent kinase inhibitor p27(KIP1)...
  16. ncbi Degradation of p57Kip2 mediated by SCFSkp2-dependent ubiquitylation
    Takumi Kamura
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, Fukuoka 812 8582, Japan
    Proc Natl Acad Sci U S A 100:10231-6. 2003
    ..The elimination of p57Kip2 was impaired in Skp2-/- cells, resulting in abnormal accumulation of the protein...
  17. ncbi Degradation of the SCF component Skp2 in cell-cycle phase G1 by the anaphase-promoting complex
    Wenyi Wei
    Department of Medical Oncology, Dana Farber Cancer Institute and Brigham and Women s Hospital, Boston, Massachusetts 02115, USA
    Nature 428:194-8. 2004
    ..The major target of the Skp1/Cul1/Skp2 (SCF(SKP2)) complex is thought to be the Cdk inhibitor p27 during S phase, whereas the principal targets for the ..
  18. ncbi Oncogenic role of the ubiquitin ligase subunit Skp2 in human breast cancer
    Sabina Signoretti
    Department of Medical Oncology, Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts, USA
    J Clin Invest 110:633-41. 2002
    ..The S-phase kinase-associated protein Skp2 is required for the ubiquitin-mediated degradation of the cdk-inhibitor p27 and is a bona fide proto-oncoprotein...
  19. ncbi E2F-1, Skp2 and cyclin E oncoproteins are upregulated and directly correlated in high-grade neuroendocrine lung tumors
    C Salon
    Equipe Bases Moléculaires de la Progression des Cancers du Poumon, Centre de Recherche INSERM U823, Institut Albert Bonniot, Grenoble Cedex, France
    Oncogene 26:6927-36. 2007
    ..linking the status of E2F-1 and a mediator of its proteasomal degradation, the S-phase kinase-associated protein 2 (Skp2) F-box protein...
  20. ncbi Relationship between levels of Skp2 and P27 in breast carcinomas and possible role of Skp2 as targeted therapy
    Wei Qiang Zheng
    Department of Pathology, Changhai Hospital, Second Military Medical University, 174 Chang Hai Road, Shanghai 200433, China
    Steroids 70:770-4. 2005
    ..The S-phase kinase-associated protein Skp2 is required for the ubiquitin-mediated degradation of the cdk-inhibitor p27 and is a bona fide proto-oncoprotein...
  21. ncbi 1,25-dihydroxyvitamin D3 transcriptionally represses p45Skp2 expression via the Sp1 sites in human prostate cancer cells
    Yu Chun Huang
    Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan, China
    J Cell Physiol 209:363-9. 2006
    ..1,25-dihydroxyvitamin D3 inhibits the expression of p45Skp2, the F-box protein which is implicated in p27Kip1 degradation, to reduce turnover of p27Kip1 protein...
  22. ncbi p27-Associated G1 arrest induced by hinokitiol in human malignant melanoma cells is mediated via down-regulation of pRb, Skp2 ubiquitin ligase, and impairment of Cdk2 function
    Shicheng Liu
    Research and Development Department, Nipro Patch Co, Ltd, Kasukabe, Saitama 344 0057, Japan
    Cancer Lett 286:240-9. 2009
    ..increased the stability of the p27 protein by inhibiting p27 phosphorylation at Thr(187) and by down-regulating Skp2 expression...
  23. ncbi Interaction between ubiquitin-protein ligase SCFSKP2 and E2F-1 underlies the regulation of E2F-1 degradation
    A Marti
    Friedrich Miescher Institut, Basel, Switzerland
    Nat Cell Biol 1:14-9. 1999
    ..This event is linked to a specific interaction of E2F-1 with the F-box-containing protein p45SKP2, which is the cell-cycle-regulated component of the ubiquitin-protein ligase SCFSKP2 that recognizes substrates ..
  24. ncbi SKP2 is required for ubiquitin-mediated degradation of the CDK inhibitor p27
    A C Carrano
    Department of Pathology, New York University Medical Center, New York 10016, USA
    Nat Cell Biol 1:193-9. 1999
    ..Here we show that the F-box protein SKP2 specifically recognizes p27 in a phosphorylation-dependent manner that is characteristic of an F-box-protein-..
  25. ncbi Prognostic impact of Skp2 and p27 in human breast cancer
    Frank Traub
    Institue of Pathology, Medizinische Hochschule Hannover, Carl Neuberg Strasse 1, D 30625, Hannover, Germany
    Breast Cancer Res Treat 99:185-91. 2006
    ..The F-box protein Skp2 is a regulator of G1-S transition and promotes specifically the ubiquitin-mediated proteolysis of p27 via the ..
  26. ncbi Skp2 regulates G2/M progression in a p53-dependent manner
    Rong Hu
    Center for Cell Biology and Cancer Research, Albany Medical College, Albany, NY 12208, USA
    Mol Biol Cell 19:4602-10. 2008
    ..S phase kinase-associated protein 2 (Skp2) is the F-box component of an E3 ubiquitin ligase complex that targets p27(Kip1) and cyclin E1 to the proteasome...
  27. ncbi Insights into SCF ubiquitin ligases from the structure of the Skp1-Skp2 complex
    B A Schulman
    Cellular Biochemistry and Biophysics Program, Medical Institute, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nature 408:381-6. 2000
    ..Here we describe the crystal structure of the human F-box protein Skp2 bound to Skp1...
  28. ncbi Role of the F-box protein Skp2 in lymphomagenesis
    E Latres
    Department of Pathology, Kaplan Comprehensive Cancer Center, New York University School of Medicine, 550 First Avenue, MSB 548, New York, NY 10016, USA
    Proc Natl Acad Sci U S A 98:2515-20. 2001
    The F-box protein Skp2 (S-phase kinase-associated protein 2) positively regulates the G(1)-S transition by controlling the stability of several G(1) regulators, such as the cell cycle inhibitor p27...
  29. ncbi Cooperation of ERK and SCFSkp2 for MKP-1 destruction provides a positive feedback regulation of proliferating signaling
    Yun Wei Lin
    Molecular Carcinogenesis Laboratory, Institute of Biotechnology, Hsinchu, Taiwan
    J Biol Chem 281:915-26. 2006
    ..Intriguingly, active ERK-promoted phospho-Ser(296) MKP-1 bound to SCF(Skp2) ubiquitin ligase in vivo and in vitro...
  30. ncbi Skp2 overexpression is highly representative of intrinsic biological aggressiveness and independently associated with poor prognosis in primary localized myxofibrosarcomas
    Hsuan Ying Huang
    Departments of Pathology and Radiation Oncology and Graduate institute of Clinical Medical Science, Chang Gung Memorial Hospital, Kaohsiung Medical Center, Chang Gung University, 123 Ta Pei Road, Niao Sung, Kaohsiung County, Taiwan
    Clin Cancer Res 12:487-98. 2006
    Two SCF(Skp2) ubiquitin ligase-related proteins, Skp2 and cyclin-dependent kinase subunit 1 (Cks1), are involved in posttranscriptional degradation of p27(Kip1) tumor suppressor...
  31. ncbi Role of the F-box protein Skp2 in adhesion-dependent cell cycle progression
    A C Carrano
    Department of Pathology and Kaplan Comprehensive Cancer Center, MSB 548, New York University School of Medicine, New York, New York 10016, USA
    J Cell Biol 153:1381-90. 2001
    ..We have reported previously that the degradation of p27 requires its phosphorylation on Thr-187 and is mediated by Skp2, an F-box protein that associates with Skp1, Cul1, and Roc1/Rbx1 to form the SCF(Skp2) ubiquitin ligase complex...
  32. ncbi The F-box protein SKP2 binds to the phosphorylated threonine 380 in cyclin E and regulates ubiquitin-dependent degradation of cyclin E
    K H Yeh
    Department of Genetics, Yale University School of Medicine, 333 Cedar Street, New Haven, Connecticut 06520, USA
    Biochem Biophys Res Commun 281:884-90. 2001
    ..We report that SKP2, an F-box protein and a substrate-targeting component of the SCF(SKP2) ubiquitin E3 ligase complex, mediates cyclin ..
  33. ncbi The cell-cycle regulatory protein Cks1 is required for SCF(Skp2)-mediated ubiquitinylation of p27
    D Ganoth
    Unit of Biochemistry, B Rappaport Faculty of Medicine, Technion Israel Institute of Technology, Haifa, 31096, Israel
    Nat Cell Biol 3:321-4. 2001
    ..its phosphorylation at Thr 187 (refs 3, 4) and subsequent recognition by S-phase kinase associated protein 2 (Skp2; refs 5-8), a member of the F-box family of proteins that associates with Skp1, Cul-1 and ROC1/Rbx1 to form an SCF ..
  34. ncbi Control of the SCF(Skp2-Cks1) ubiquitin ligase by the APC/C(Cdh1) ubiquitin ligase
    Tarig Bashir
    Department of Pathology, MSB 599, New York University School of Medicine, and New York University Cancer Institute, 550 First Avenue, New York, New York 10016, USA
    Nature 428:190-3. 2004
    b>Skp2 and its cofactor Cks1 are the substrate-targeting subunits of the SCF(Skp2-Cks1) (Skp1/Cul1/F-box protein) ubiquitin ligase complex that regulates entry into S phase by inducing the degradation of the cyclin-dependent kinase ..
  35. ncbi Regulation of neddylation and deneddylation of cullin1 in SCFSkp2 ubiquitin ligase by F-box protein and substrate
    Gil Bornstein
    Unit of Biochemistry, Rappaport Faculty of Medicine, Technion Israel Institute of Technology, Haifa 31096, Israel
    Proc Natl Acad Sci U S A 103:11515-20. 2006
    ..We examined this problem for the case of SCF(Skp2), a cullin1 (Cul1)-containing ubiquitin ligase complex that contains the S phase-associated protein Skp2 as the ..
  36. ncbi Correlation of Skp2 with carcinogenesis, invasion, metastasis, and prognosis in colorectal tumors
    Jia Qing Li
    Department of Surgery, Vanderbilt University Medical Center, Nashville, TN 37232 2733, USA
    Int J Oncol 25:87-95. 2004
    In colorectal tumors, S-phase kinase-associated protein 2 (Skp2) still has numerous important questions unanswered: its expression in adenomas, its correlation with key clinicopathological indices, its association with patient prognosis, ..
  37. ncbi The F-box protein Skp2 participates in c-Myc proteosomal degradation and acts as a cofactor for c-Myc-regulated transcription
    Natalie von der Lehr
    Department of Plant Biology and Forest Genetics, Swedish University of Agricultural Sciences, 750 07 Uppsala, Sweden
    Mol Cell 11:1189-200. 2003
    ..The proteins involved in this process are still unknown. We have found that Skp2 interacts with c-Myc and participates in its ubiquitylation and degradation...
  38. ncbi Mutant B-RAF signaling and cyclin D1 regulate Cks1/S-phase kinase-associated protein 2-mediated degradation of p27Kip1 in human melanoma cells
    K V Bhatt
    Center for Cell Biology and Cancer Research, 47 New Scotland Avenue, Albany Medical College, Albany, NY 12208, USA
    Oncogene 26:1056-66. 2007
    ..Additionally, B-RAF and cyclin D1 control the levels of S-phase kinase-associated protein 2 (Skp2) that directs ubiquitin-mediated proteolysis of p27(Kip1)...
  39. ncbi Dual-specificity phosphatase 1 ubiquitination in extracellular signal-regulated kinase-mediated control of growth in human hepatocellular carcinoma
    Diego F Calvisi
    Department of Biomedical Sciences, Division of Experimental Pathology and Oncology, University of Sassari, 07100 Sassari, Italy
    Cancer Res 68:4192-200. 2008
    ..with dual-specificity phosphatase 1 (DUSP1), a specific inhibitor of ERK, and S-phase kinase-associated protein 2 (SKP2)/CDC28 protein kinase 1b (CKS1) ubiquitin ligase complex in human hepatocellular carcinoma (HCC)...
  40. ncbi Structure of the Cul1-Rbx1-Skp1-F boxSkp2 SCF ubiquitin ligase complex
    Ning Zheng
    Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, New York 10021, USA
    Nature 416:703-9. 2002
    ..The structure suggests that Cul1 may contribute to catalysis through the positioning of the substrate and the ubiquitin-conjugating enzyme, and this model is supported by Cul1 mutations designed to eliminate the rigidity of the scaffold...
  41. ncbi Association of human CUL-1 and ubiquitin-conjugating enzyme CDC34 with the F-box protein p45(SKP2): evidence for evolutionary conservation in the subunit composition of the CDC34-SCF pathway
    J Lisztwan
    Friedrich Miescher Institut, Basel, Switzerland
    EMBO J 17:368-83. 1998
    In normal and transformed cells, the F-box protein p45(SKP2) is required for S phase and forms stable complexes with p19(SKP1) and cyclin A-cyclin-dependent kinase (CDK)2...
  42. ncbi CAND1 binds to unneddylated CUL1 and regulates the formation of SCF ubiquitin E3 ligase complex
    Jianyu Zheng
    Department of Genetics, Yale University School of Medicine, 333 Cedar Street, Yale University, New Haven, CT 06520, USA
    Mol Cell 10:1519-26. 2002
    ..We found the majority of CUL1 is in a complex with CAND1 and ROC1 independent of SKP1 and F box protein SKP2. Both in vivo and in vitro, CAND1 prevents the binding of SKP1 and SKP2 to CUL1 while dissociation of CAND1 from ..
  43. ncbi Liver X receptor agonists suppress vascular smooth muscle cell proliferation and inhibit neointima formation in balloon-injured rat carotid arteries
    Florian Blaschke
    Division of Endocrinology, Diabetes, and Hypertension, David Geffen School of Medicine, University of California, Los Angeles, USA
    Circ Res 95:e110-23. 2004
    ..LXR ligands was mediated by supressing the transcriptional activation of the S phase kinase-associated protein 2 (Skp2), an F-box protein that targets p27Kip1 for degradation...
  44. ncbi Utility of SKP2 and MIB-1 in grading follicular lymphoma using quantitative imaging analysis
    Kai Zhang
    Department of Laboratory Medicine, Geisinger Medical Center, Danville, PA 17822, USA
    Hum Pathol 38:878-82. 2007
    ..with immunostains with antibodies to proliferation markers MIB-1 and S-phase kinase-associated protein 2 (SKP2)...
  45. ncbi Overexpression of cyclin E messenger ribonucleic acid in nasopharyngeal carcinoma correlates with poor prognosis
    M T Ko
    Department of Otolaryngology, Chang Gung Memorial Hospital Kaohsiung Medical Center, Chang Gung University College of Medicine, Kaohsiung, Taiwan
    J Laryngol Otol 123:1021-6. 2009
    ..The present study examined the expression of cyclin E, S-phase kinase-associated protein 2 and p27 protein in nasopharyngeal carcinoma...
  46. ncbi The E3 ubiquitin ligase skp2 regulates neural differentiation independent from the cell cycle
    Hector Boix-Perales
    Department of Oncology, University of Cambridge, Hutchison MRC Research Centre, Addenbrookes Hospital, Hills Road, Cambridge CB2 0XZ, UK
    Neural Dev 2:27. 2007
    ..While its role in regulation of cell division has been well documented, additional functions in differentiation, including in the nervous system, have not been investigated...
  47. ncbi [Expression of S-phase kinase associated protein 2 in laryngeal carcinoma and its molecular mechanisms involved in regulation of proliferation and apoptosis of Hep-2 cells]
    Xiu Ying Lu
    Department of Otorhinolaryngology Head and Neck Surgery, Bethune International Peace Hospital of People s Liberation Army, Shijiazhuang 050082, China
    Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 44:681-6. 2009
    To investigate the expression of S-phase kinase associated protein 2 (Skp2) in human laryngeal squamous cell carcinomas and to explore the effect of silencing of Skp2 by RNAi on the proliferation and apoptosis and the expressing of p27 ..
  48. ncbi [Expressions of Cx43 and Skp2 in epithelial ovarian tumor and their clinical significances]
    Ye qing Hu
    Department of Gynecology and Obstetrics, People s Hospital, Wuhan University, Wuhan, Hubei 430 060, P R China
    Ai Zheng 24:104-9. 2005
    ..that Cx43 could inhibit tumor growth via down-regulating the expression of S-phase kinase associated protein 2 (Skp2)...
  49. ncbi Prognostic significance of p27Kip1, p45Skp2 and Ki67 expression profiles in Merkel cell carcinoma, extracutaneous small cell carcinoma, and cutaneous squamous cell carcinoma
    M T Fernandez-Figueras
    Department of Pathology, Hospital Universitari Germans Trias i Pujol, Catalonia, Spain
    Histopathology 46:614-21. 2005
    To compare the immunohistochemical expression of prognostic markers p27(Kip1), p45(Skp2) and Ki67 in Merkel cell carcinoma (primary neuroendocrine carcinoma of the skin, MCC), small cell neuroendocrine carcinoma of lung and urinary ..
  50. ncbi Apigenin induces apoptosis via downregulation of S-phase kinase-associated protein 2-mediated induction of p27Kip1 in primary effusion lymphoma cells
    A R Hussain
    Human Cancer Genomic Research, Research Center, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia
    Cell Prolif 43:170-83. 2010
    ..In efforts to identify novel approaches to block the proliferation of PEL cells, we assessed the effect of apigenin (4',5,7-trihydroxyflavone), a flavonoid on a panel of PEL cell lines...
  51. ncbi S-phase kinase-associated protein-2 (Skp2) promotes vascular smooth muscle cell proliferation and neointima formation in vivo
    Yih Jer Wu
    Department of Cardiovascular Medicine and Medical Research, Mackay Memorial Hospital, and Department of Medicine, Mackay Medical College, and Institute of Traditional Medicine, National Yang Ming University, Taipei, Taiwan
    J Vasc Surg 50:1135-42. 2009
    ..Our previous work has demonstrated S-phase kinase-associated protein-2 (Skp2), an F-box subunit of SCF(Skp2) ubiquitin ligase, as an important mediator and common final pathway for growth ..
  52. ncbi Ubiquitination of p21Cip1/WAF1 by SCFSkp2: substrate requirement and ubiquitination site selection
    Wei Wang
    Department of Chemistry and Biochemistry, University of Colorado Boulder, Boulder, Colorado 80309, USA
    Biochemistry 44:14553-64. 2005
    ..The SCF(Skp2) complex (consisting of Rbx1, Cul1, Skp1, and Skp2) is one of the E3 ubiquitin ligases involved in ubiquitination ..
  53. ncbi Down-regulation of S-phase kinase associated protein 2 (Skp2) induces apoptosis in oral cancer cells
    Koji Harada
    Second Department of Oral and Maxillofacial Surgery, School of Dentistry, University of Tokushima, 3 18 15 Kuramoto cho, Tokushima 770 8504, Japan
    Oral Oncol 41:623-30. 2005
    S-phase kinase associated protein 2 (Skp2) is a member of an F-box family of substrate-recognition subunits of SCF ubiquitin-protein ligase complexes that has been implicated in the ubiquitin-mediated degradation of several key regulators ..
  54. ncbi [Expression of SKP2 protein in lung carcinoma and its implication for prognosis]
    Jun Zhao
    Department of Thoracic Surgery, The First Affiliated Hospital of China Medical Univercity, Shenyang 110001, China
    Zhonghua Zhong Liu Za Zhi 29:289-92. 2007
    To investigate the characteristics of SKP2 protein expression in lung carcinoma tissues and its implication for prognosis.
  55. ncbi Inhibition of proliferation of rabbit lens epithelial cells by S-phase kinase-interacting protein 2 targeting small interfering RNA
    Ying Su
    Department of Ophthalmology, First Clinic College of Harbin Medical University, Harbin, China
    Mol Vis 16:907-15. 2010
    ..we investigated whether small interfering RNA (siRNA)-mediated gene silencing of S-phase kinase-interacting protein 2 (Skp2) can be employed to inhibit rabbit lens epithelial cell (rLEC) proliferation by increasing the p27(kip1) level.
  56. ncbi Partial molecular characterization, expression pattern, polymorphism and association analysis of porcine SKP2 gene
    Y Li
    College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, People s Republic of China
    Mol Biol Rep 37:1309-17. 2010
    As a component of E3 ubiquitin protein ligases called SCFs, SKP2 protein belongs to a member of FBLs protein which is the biggest eukaryotic subfamily of F-BOX proteins with 12 members...
  57. ncbi Reduced XPC messenger RNA level may predict a poor outcome of patients with nonsmall cell lung cancer
    Yi Hui Wu
    Institute of Medical and Molecular Toxicology, Chung Shan Medical University, Taichung, Taiwan, Republic of China
    Cancer 110:215-23. 2007
    ..The current study attempted to determine whether reduced XPC mRNA levels predict the clinical outcome of lung cancer patients...
  58. ncbi p27 Kip1 nuclear localization and cyclin-dependent kinase inhibitory activity are regulated by glycogen synthase kinase-3 in human colon cancer cells
    Q Wang
    Department of Surgery, The University of Texas Medical Branch, Galveston, TX 77555 0536, USA
    Cell Death Differ 15:908-19. 2008
    ..In addition, we demonstrate that NaBT decreased the expression of S-phase kinase-associated protein 2 (Skp2), and this decrease was attenuated by GSK-3 inhibition...
  59. ncbi The gap junction-independent tumor-suppressing effect of connexin 43
    You Wei Zhang
    Department of Cellular Physiological Chemistry, Tokyo Medical and Dental University, 1 5 45 Yushima, Bunkyo ku, Tokyo 113 8549, Japan
    J Biol Chem 278:44852-6. 2003
    ..We have previously demonstrated that Cx43 inhibited expression of S phase kinase-associated protein 2 (Skp2), the human F-box protein that regulates the ubiquitination of p27...
  60. ncbi PAX3-FKHR transformation increases 26 S proteasome-dependent degradation of p27Kip1, a potential role for elevated Skp2 expression
    Lei Zhang
    Center for Molecular Biology of Oral Diseases, University of Illinois at Chicago, Chicago, Illinois 60612, USA
    J Biol Chem 278:27-36. 2003
    ..Along with the reduction in p27(Kip1) protein, PAX3-FKHR-expressing cells exhibited elevated expression of F-box Skp2 protein, a substrate-specific component of SCF (Skp1-Cullin-F box protein) ligase involved in the cell cycle-..
  61. ncbi Vitamin D inhibits G1 to S progression in LNCaP prostate cancer cells through p27Kip1 stabilization and Cdk2 mislocalization to the cytoplasm
    Eddy S Yang
    Department of Molecular and Cellular Pharmacology, University of Miami School of Medicine, Miami, FL 33136, USA
    J Biol Chem 278:46862-8. 2003
    ..Because Cdk2-mediated phosphorylation of p27 at Thr187 targets p27 for Skp2-mediated degradation, we examined the phosphorylation status of p27 in 1,25-(OH)2D3-treated cells...
  62. ncbi [Effect of Skp2 antisense oligodeoxynucleotide on growth and proliferation of gastric carcinoma SGC-7901 cells]
    Lin hai Shen
    Department of Biochemistry and Genetics, College of Medicine, Zhejiang University, Hangzhou 310058, China
    Zhejiang Da Xue Xue Bao Yi Xue Ban 37:182-8. 2008
    To investigate the effect of S-phase kinase-associated protein 2 antisense oligodeoxynucleotide (Skp2 ASODN) on the growth and proliferation of gastric carcinoma SGC-7901 cells and its mechanism.
  63. ncbi High expression of S-phase kinase-associated protein 2 (Skp2) is a strong prognostic marker in oral squamous cell carcinoma patients treated by UFT in combination with radiation
    Koji Harada
    Second Department of Oral and Maxillofacial Surgery, University of Tokushima, School of Dentistry, 3 18 15 Kuramoto cho, Tokushima 770 8504, Japan
    Anticancer Res 25:2471-5. 2005
    ..In addition, p27(Kip1) protein is thought to be degraded by Skp2 (S-phase kinase-associated protein 2)...
  64. ncbi Overexpression of Jun activation domain-binding protein 1 in nonsmall cell lung cancer and its significance in p27 expression and clinical features
    Atsushi Osoegawa
    Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan
    Cancer 107:154-61. 2006
    ..in p27 degradation in a manner that is independent from the role played by S-phase kinase-associated protein 2 (Skp2)...
  65. ncbi Hormonal induction of adipogenesis induces Skp2 expression through PI3K and MAPK pathways
    Corinth A Auld
    Department of Nutrition, The University of North Carolina at Greensboro, Greensboro, North Carolina 27402, USA
    J Cell Biochem 100:204-16. 2007
    We have previously shown that the F-box protein, S-phase kinase-associated protein (Skp2) plays a mechanistic role in targeting the cell-cycle inhibitor, p27 for degradation by the 26S proteasome during early stages of 3T3-L1 adipocyte ..
  66. ncbi Bcr-Abl-induced tyrosine phosphorylation of Emi1 to stabilize Skp2 protein via inhibition of ubiquitination in chronic myeloid leukemia cells
    Jing Yi Chen
    Institute of Biomedical Sciences, National Sun Yat Sen University, Kaohsiung, Taiwan
    J Cell Physiol 226:407-13. 2011
    ..demonstrates that Bcr-Abl fusion oncogene frequently found in chronic myeloid leukemia (CML) cells can up-regulate Skp2 expression via transcriptional activation. However, Bcr-Abl also modulates Skp2 protein stability in these cells...
  67. ncbi Prognostic significance of S-phase kinase-associated protein 2 and p27kip1 in patients with diffuse large B-cell lymphoma: effects of rituximab
    R Seki
    Division of Hematology and Oncology, Department of Medicine, Kurume University School of Medicine, 67 Asahi Machi, Kurume, Fukuoka, Japan
    Ann Oncol 21:833-41. 2010
    The F-box protein S-phase kinase-associated protein 2 (Skp2) positively regulates the G1-S transition by promoting degradation of the cyclin-dependent kinase inhibitor p27(kip1) (p27)...
  68. ncbi The F box protein S phase kinase-associated protein 2 regulates adipose mass and adipocyte number in vivo
    Paul S Cooke
    Department of Veterinary Biosciences, 2001 South Lincoln Ave, University of Illinois at Urbana Champaign, Urbana, IL 61802, USA
    Obesity (Silver Spring) 15:1400-8. 2007
    ..proteins such as p27 for ubiquitin-proteosome degradation; the F box protein S phase kinase-associated protein 2 (Skp2), a component of the SCF complex, specifically recognizes p27 for degradation...
  69. ncbi Inhibition of cell proliferation of Tenon's capsule fibroblast by S-phase kinase-interacting protein 2 targeting SiRNA through increasing p27 protein level
    Feng Wang
    Department of Ophthalmology, First Clinical College, Harbin Medical University, Harbin, China
    Invest Ophthalmol Vis Sci 51:1475-82. 2010
    ..In the present study, the authors investigated whether small interfering RNA (siRNA)-mediated gene silencing of Skp2 can be used to increase p27(kip1) level and inhibit cell proliferation in rabbit Tenon's capsule fibroblast (rTF).
  70. ncbi Role of the SCFSkp2 ubiquitin ligase in the degradation of p21Cip1 in S phase
    Gil Bornstein
    Unit of Biochemistry, The B Rappaport Faculty of Medicine, Technion Israel Institute of Technology, Haifa 31096, Israel
    J Biol Chem 278:25752-7. 2003
    ..a good substrate for an SCF (Skp1-Cullin1-F-box protein) ubiquitin ligase complex, which contains the F-box protein Skp2 (S phase kinase-associated protein 2) and the accessory protein Cks1 (cyclin kinase subunit 1)...
  71. ncbi STAT1 represses Skp2 gene transcription to promote p27Kip1 stabilization in Ras-transformed cells
    Shuo Wang
    Lady Davis Institute for Medical Research, Sir Mortimer B Davis Jewish General Hospital, Room 508, 3999 Cote Ste Catherine Road, Montreal, Quebec, Canada H3T 1E2
    Mol Cancer Res 8:798-805. 2010
    The S-phase kinase-associated protein 2 (Skp2) is an F-box protein that serves as a subunit of the Skp1-Cullin-F-box ubiquitin protein ligase complex...
  72. ncbi A novel route for connexin 43 to inhibit cell proliferation: negative regulation of S-phase kinase-associated protein (Skp 2)
    You Wei Zhang
    Department of Cellular Physiological Chemistry, Tokyo Medical and Dental University, Tokyo 113 8549, Japan
    Cancer Res 63:1623-30. 2003
    ..W., et al., Oncogene, 20: 4138-4149, 2001). Here we report that Cx43 inhibited expression of Skp2, the human F-box protein that regulates p27 ubiquitination...
  73. ncbi EGCG stabilizes p27kip1 in E2-stimulated MCF-7 cells through down-regulation of the Skp2 protein
    Hsiu Chen Huang
    Institute of Biochemistry and Molecular Biology, National Taiwan University Medicine College, Taipei 100, Taiwan
    Endocrinology 149:5972-83. 2008
    ..proteasome-dependent degradation mediated by its specific ubiquitin ligase subunit S-phase kinase protein 2 (Skp2)...
  74. ncbi Rho GTPase, Rac1, regulates Skp2 levels, vascular smooth muscle cell proliferation, and intima formation in vitro and in vivo
    Mark Bond
    Bristol Heart Institute, University of Bristol, Level 7, Bristol Royal Infirmary, Bristol BS2 8HW, UK
    Cardiovasc Res 80:290-8. 2008
    ..VSMC proliferation requires degradation of p27(Kip1) promoted by S-phase kinase-associated protein-2 (Skp2), an F-box protein component of the Skp-Cullin-F-box(Skp2) ubiquitin-ligase...
  75. ncbi Cyclin-dependent kinase 1 gene expression is associated with poor prognosis in gastric carcinoma
    Taka aki Masuda
    Department of Molecular and Surgical Oncology, Medical Institute of Bioregulation, Kyushu University, Beppu, Japan
    Clin Cancer Res 9:5693-8. 2003
    ..Human Cdk subunit 1 (Cks1), as well as S-phase kinase-associated protein 2 (Skp2), is an essential and specific factor in the p27 proteolysis by SCF(Skp2) ubiquitin ligase...
  76. ncbi Dynamic changes of p27(kip1) and Skp2 expression in injured rat sciatic nerve
    Ai guo Shen
    The Jiangsu Province Key Laboratory of Neuroregeneration, Nantong University, Nantong, P R China
    Cell Mol Neurobiol 28:713-25. 2008
    S phase kinase-associated protein 2 (Skp2), an F-box protein, is required for the ubiquitination and consequent degradation of p27(kip1)...
  77. ncbi Effects of wortmannin on phosphorylation of PDK1, GSK3-beta, PTEN and expression of Skp2 mRNA after ischemia/reperfusion injury in the mouse kidney
    Xiangyi Zheng
    Department of Urology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, Zhejiang, China
    Int Urol Nephrol 40:185-92. 2008
    ..homologue deleted on chromosome 10 (PTEN), which are the components of the phosphoinositide 3-kinase (PI3K)/Akt pathway, and expression of S-phase kinase-associated protein 2 (Skp2) messenger RNA (mRNA) in renal ischemia/reperfusion.
  78. ncbi Hemangiomas and angiosarcomas of the breast: diagnostic utility of cell cycle markers with emphasis on Ki-67
    Sandra J Shin
    Department of Pathology and Laboratory Medicine Starr 1009, New York Presbyterian Hospital Weill Medical College of Cornell University, 525 E 68th St, New York, NY 10021, USA
    Arch Pathol Lab Med 131:538-44. 2007
    ..Ki-67 expression has been used as an adjunct to distinguish between benign and malignant lesions exhibiting histologic overlap at various anatomic sites...
  79. ncbi Cell adhesion induces p27Kip1-associated cell-cycle arrest through down-regulation of the SCFSkp2 ubiquitin ligase pathway in mantle-cell and other non-Hodgkin B-cell lymphomas
    Tint Lwin
    Department of Interdisciplinary Oncology and Experimental Therapeutics Program, H Lee Moffitt Cancer Center and Research Institute at the University of South Florida, Tampa, FL 33613, USA
    Blood 110:1631-8. 2007
    ..The adhesion-mediated p27(Kip1) and p21 increases were posttranslationally regulated via the down-regulation of Skp2, a subunit of SCF(Skp2) ubiquitin ligase...
  80. ncbi Phosphoinositide-3-kinase signaling controls S-phase kinase-associated protein 2 transcription via E2F1 in pancreatic ductal adenocarcinoma cells
    Maximilian Reichert
    Department of Internal Medicine, Technical University of Munich, Munich, Germany
    Cancer Res 67:4149-56. 2007
    ..of G(1)-S phase progression controlled by PI3K signaling like p21(Cip1), S-phase kinase-associated protein 2 (SKP2), CDC25a, cyclin A, cyclin D2, CDK2, and cyclin E...
  81. ncbi CD28 costimulation mediates transcription of SKP2 and CKS1, the substrate recognition components of SCFSkp2 ubiquitin ligase that leads p27kip1 to degradation
    Leonard J Appleman
    Department of Medical Oncology, Dana Farber Cancer Institute, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA
    Cell Cycle 5:2123-9. 2006
    ..Ubiquitination of p27(kip1) is performed by the SCF(skp2) ubiquitin ligase comprised of the core components Roc1, Cul1 and Skp1 and the substrate recognition components ..
  82. ncbi Knockdown of Skp2 by siRNA inhibits melanoma cell growth in vitro and in vivo
    Yoshiyuki Katagiri
    Department of Dermatology, Yamagata University School of Medicine, 2 2 2 Iida nishi, 990 9585 Yamagata, Japan
    J Dermatol Sci 42:215-24. 2006
    ..Recently, it has been reported that S phase kinase-interacting protein 2 (Skp2), the specific ubiquitin ligase subunit that targets p27Kip1 for degradation, was overexpressed and was inversely ..
  83. ncbi p27 Kip1 protein expression in Hashimoto's thyroiditis
    G Troncone
    Dipartimento di Scienze Biomorfologiche e Funzionali, University Federico II, 80131 Naples, Italy
    J Clin Pathol 56:587-91. 2003
    ..Because p27(Kip1) is regulated through either degradation, mediated by the S phase kinase associated protein 2 (Skp2), or sequestration, via D3 cyclin, the expression of these proteins was also investigated.
  84. ncbi Suppression of S-phase kinase-associated protein 2 induces apoptosis and inhibits tumor growth in human laryngeal cancer
    Jiangtao Liu
    Department of Otorhinolaryngology, China Medical University Affiliated Shengjing Hospital, Shenyang, China
    ORL J Otorhinolaryngol Relat Spec 72:205-14. 2010
    S-phase kinase-associated protein 2 (Skp2) is related to cellular proliferation and differentiation in laryngeal carcinoma...
  85. ncbi [Expression of Skp2 and PTEN and its correlation in glottic carcinoma]
    Hongchun Zhao
    Department of Otolaryngology Head and Neck Surgery, The Affiliated Hospital of Binzhou Medical College, Binzhou 256603, China
    Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 22:688-91. 2008
    To investigate the expression and clinical significance of Skp2 and PTEN in glottic carcinoma and the relationship between the two genes.
  86. ncbi Exendin-4 stimulation of cyclin A2 in beta-cell proliferation
    Woo Jin Song
    Metabolism Division, Departments of Pediatrics and Medicine, Johns Hopkins University, Baltimore, Maryland, USA
    Diabetes 57:2371-81. 2008
    ..We have examined effects, in particular those mediated through intracellular cAMP signaling, of the incretin hormone analog exendin-4 on cell cycle regulation in beta-cells...
  87. ncbi [Clinical significance of SKP2 and MRP-1/CD9 expression in glottic carcinoma and adjacent tissues]
    Yan Wang
    Department of Otorhinolaryngology, The Affiliated Hospital of Medical College of Qingdao University, Qingdao, 266003, China
    Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 24:158-60, 165. 2010
    To study the expression of SKP2 and MRP-1/CD9 protein in glottic cancer and adjacent tissues, and to analyze its significance for a safe surgical margin.
  88. ncbi Morphoproteomic profile of mTOR, Ras/Raf kinase/ERK, and NF-kappaB pathways in human gastric adenocarcinoma
    Wei Feng
    Department of Pathology, MD Anderson Cancer Center, Houston, TX 77030, USA
    Ann Clin Lab Sci 38:195-209. 2008
    ..The majority of cases showed concomitant moderate-strong (2+ to 4+) nuclear Ki-67 (71%) and Skp2 (68%)...
  89. ncbi Focal adhesion kinase (FAK)-dependent regulation of S-phase kinase-associated protein-2 (Skp-2) stability. A novel mechanism regulating smooth muscle cell proliferation
    Mark Bond
    Bristol Heart Institute, University of Bristol, Bristol BS2 8HW, United Kingdom
    J Biol Chem 279:37304-10. 2004
    ..Taken together, these data demonstrate that the vascular ECM controls SMC proliferation via FAK-dependent regulation of Skp-2 protein stability...
  90. ncbi Novel effect of helenalin on Akt signaling and Skp2 expression in 3T3-L1 preadipocytes
    Corinth A Auld
    Department of Nutrition, The University of North Carolina at Greensboro, Greensboro, NC 27402, USA
    Biochem Biophys Res Commun 346:314-20. 2006
    We have previously shown that the F-box protein, Skp2, is highly regulated during preadipocyte proliferation and plays a mechanistic role in p27 degradation during cell cycle progression...
  91. ncbi Regulation of Skp2 expression and activity and its role in cancer progression
    Chia Hsin Chan
    1Department of Molecular and Cellular Oncology, The University of Texas M D Anderson Cancer Center, Houston, TX, USA
    ScientificWorldJournal 10:1001-15. 2010
    ..One of the key players regulating cell cycle progression is the F-box protein Skp2. Skp2 forms a SCF complex with Skp1, Cul-1, and Rbx1 to constitute E3 ligase through its F-box domain...
  92. ncbi Dibutyryl cAMP stimulates the proliferation of SH-SY5Y human neuroblastoma cells by up-regulating Skp2 protein
    Chin Ho Cho
    Department of Biochemistry and Molecular Biology, Laboratory of Cellular Signaling, Cancer Research Institute, Seoul National University College of Medicine, 28 Yongon Dong, Jongno gu, Seoul, 110 799, South Korea
    J Cancer Res Clin Oncol 133:135-44. 2007
    ..Therefore, this study was performed to investigate the mechanism whereby cAMP stimulates the proliferation of SH-SY5Y cells...
  93. ncbi Notch1 modulates timing of G1-S progression by inducing SKP2 transcription and p27 Kip1 degradation
    Leonor M Sarmento
    Center of Regenerative Medicine and Technology, Massachusetts General Hospital, Boston, MA, USA
    J Exp Med 202:157-68. 2005
    ..and ligand-dependent activation of Notch1 induces transcription of the S phase kinase-associated protein 2 (SKP2), the F-box subunit of the ubiquitin-ligase complex SCF(SKP2) that targets proteins for degradation...
  94. ncbi SKP2 confers resistance of pancreatic cancer cells towards TRAIL-induced apoptosis
    Susanne Schüler
    II Medizinische Klinik, Technische Universitat Munchen, Ismaninger Str 22, D 81675 Munchen, Germany
    Int J Oncol 38:219-25. 2011
    ..Although the F-box protein S-phase kinase associated protein 2 (SKP2) is highly expressed and regulates cell cycle progression in PDAC, alternative SKP2 functions in PDAC are unknown...
  95. ncbi Functional significance of cytochrome P450 1B1 in endometrial carcinogenesis
    Sharanjot Saini
    Department of Urology, Veterans Affairs Medical Center and University of California San Francisco, San Francisco, California 94121, USA
    Cancer Res 69:7038-45. 2009
    ..CYP1B1 knockdown leads to down-regulated expression of cyclin E1, S-phase kinase-associated protein 2 (SKP2), minichromosome maintenance complex component 4 (MCM4), and RAD51 and up-regulation of p27(Kip1)...
  96. ncbi Correlation between Skp2 expression and nodal metastasis in stage I and II oral squamous cell carcinomas
    P Tosco
    Division of Maxillo Facial Surgery, Department of Clinical Physiopathology, S Giovanni Battista Hospital, University of Turin, Torino, Italy
    Oral Dis 17:102-8. 2011
    The aim of this study was to investigate the role of S-phase kinase associated protein (Skp2) in the development of nodal metastasis and to assess its influence on prognosis in stage I and II oral squamous cell carcinomas (OSCCs).
  97. ncbi HDAC3 is linked to cell cycle machinery in MiaPaCa2 cells by regulating transcription of skp2
    G Schneider
    Department of Internal Medicine II, Technical University of Munich, Munich, Germany
    Cell Prolif 40:522-31. 2007
    ..Histone deacetylases (HDACs) have been linked to cell cycle control in various models, involving regulation of the cyclin-dependent kinase inhibitor p27(Kip1)...
  98. ncbi [Detection of Skp2 and p27kip1 expression in human renal cell carcinoma using tissue chip technique]
    Chen Yao
    Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China
    Nan Fang Yi Ke Da Xue Xue Bao 28:642-5. 2008
    To detect the expression of skp2 and p27kip1 in human renal cell carcinoma (RCC) using tissue chip technique, and evaluate the relationship between the proteins and the biological behavior of RCC.
  99. ncbi Aberrant expression of p27(Kip1)-interacting cell-cycle regulatory proteins in ovarian clear cell carcinomas and their precursors with special consideration of two distinct multistage clear cell carcinogenetic pathways
    Sohei Yamamoto
    Department of Basic Pathology, National Defense Medical College, 3 2 Namiki, Tokorozawa, Saitama, 359 8513, Japan
    Virchows Arch 455:413-22. 2009
    ..In this study, the expressions of p27(Kip1)-interacting proteins, i.e., p27(Kip1), Skp2, Cks1, cyclin A, cyclin E, and the Ki-67 labeling index (LI), were analyzed in 25 CCAFs (11 benign and 14 ..
  100. ncbi Analysis of the role of COP9 Signalosome (CSN) subunits in K562; the first link between CSN and autophagy
    Claire Pearce
    College of Life and Environmental Sciences, School of Biosciences, University of Birmingham, Edgbaston, Birmingham, UK
    BMC Cell Biol 10:31. 2009
    ..Although studied in model human cell lines such as HeLa, very little is known about the role of the CSN in haemopoietic cells...
  101. ncbi Modification of cullin-1 by ubiquitin-like protein Nedd8 enhances the activity of SCF(skp2) toward p27(kip1)
    M Morimoto
    School of Life Science, Tokyo University of Pharmacy and Life Science, 1432 1 Horinouchi, Hachioji, Tokyo, 192 0392, Japan
    Biochem Biophys Res Commun 270:1093-6. 2000
    ..The ubiquitin ligase (E3) toward p27(kip1) is thought to be SCF(skp2). The activity of SCF(skp2) was increased by the addition of Roc1 protein to the complex...

Research Grants68

  1. F-box proteins: deregulated cell cycle control and proteolysis in cancer
    Michele Pagano; Fiscal Year: 2007
    ..Significantly, of the three characterized F-box proteins, Skp2 is the product of a proto-oncogene, Fbw7 is a tumor suppressor, and overexpression of ?Trcp contributes to ..
  2. B-RAF Regulation of the Cell Cycle in Melanoma
    Andrew E Aplin; Fiscal Year: 2010
    ..Our preliminary data indicate that mutant B-RAF signaling and cyclin D1 regulate p27Kip1 via expression of Skp2 F- box protein, a component of a Skp1/Cullin/F-box (SCF) ubiquitin ligase complex that mediates p27Kip1 degradation...
  3. Cell Cycle Regulation and Adult T Cell Leukemia
    Chou Zen Giam; Fiscal Year: 2010
    ..the premature poly-ubiquitination and degradation of mitotic regulators including cyclin A, cyclin B, securin, and Skp2. Skp2 is the substrate-targeting subunit of another E3 ubiquitin ligase known as SCFSkp2, which mediates the ..
  4. HTLV-I Tax activates the anaphase promoting complex
    Chou Zen Giam; Fiscal Year: 2010
    ..premature poly- ubiquitination and degradation of cell cycle regulators including cyclin A, cyclin B, securin, and Skp2. Skp2 is the substrate-targeting subunit of another E3 ligase known as SCFSkp2, which mediates the destruction of ..
  5. ECM MATRIX, PROTEASE AND MELANOMA PROLIFERATION
    Yves DeClerck; Fiscal Year: 2007
    ..now obtained evidence that up-regulation of p27KIP1 is the consequence of a down-regulation of the S phase kinase associated protein 2 (Skp2) that binds p27KIP1 to ubiquitin conjugase and targets it for degradation by proteasomes...
  6. Physiologic Regulation of Hematopoiesis by Notch
    Nadia Carlesso; Fiscal Year: 2010
    ..We discovered that Notch1 activation induces the transcriptional activation of SKP2, the F-box subunit of the SCFskp2 complex that targets the cell cycle inhibitors p27Kip1 and p21Cip1 for ..
  7. Physiologic Regulation of Hematopoiesis by Notch
    Nadia Carlesso; Fiscal Year: 2009
    ..We discovered that Notch1 activation induces the transcriptional activation of SKP2, the F-box subunit of the SCFskp2 complex that targets the cell cycle inhibitors p27Kip1 and p21Cip1 for ..
  8. Regulation of Bladder Smooth Muscle Hyperplasia
    Paul Austin; Fiscal Year: 2007
    ..The candidate has also determined that the upregulation of a second protein, Skp2 is necessary for both the downregulation of p27KIP1 and the bladder SMC hyperplastic response...
  9. Regulation of the G1 Phase by the Ubiquitin Pathway
    Michele Pagano; Fiscal Year: 2005
    ..It was demonstrated by the principal investigator that the F-box protein Skp2 is a rate-limiting component of the machinery that ubiquitinylates the cell cycle inhibitor p27, and that Skp2 ..
  10. Transformation of human mammary epithelium by c-Myc
    Robert Dickson; Fiscal Year: 2005
    ..c-Myc protein expression, negatively with p27, and positively with expression of mediators of p27 degradation: SCF SKP2 ubiquitin ligase and Jab1...
  11. Transformation of human mammary epithelium by c-Myc
    Michael Johnson; Fiscal Year: 2007
    ..c-Myc protein expression, negatively with p27, and positively with expression of mediators of p27 degradation: SCF SKP2 ubiquitin ligase and Jab1...
  12. Mechanisms of p27Kip1 Proteolysis in Cancer Cells
    Xuedong Liu; Fiscal Year: 2007
    ..Consistent with this view was the finding that an elevated level of Skp2 was detected in several types of human tumors...
  13. Optimized High-Throughput Screen for Small Molecule Inhibitors of P27 Degradation
    Dieter Wolf; Fiscal Year: 2007
    ..P27 degradation is mediated by SCFSKP2-dependent polyubiquitylation and SKP2 overexpression is sufficient to cause ectopic p27 degradation...
  14. ANDROGEN RECEPTOR REGULATION OF REPRODUCTIVE FUNCTION
    Elizabeth M Wilson; Fiscal Year: 2010
    ..Aim 2 pursues evidence for a phosphorylation-dependent interaction between MAGE-11 and the F-box protein Skp2 (S phase kinase-associated protein 2) to test our hypothesis that MAGE-11 functions as a subunit of the SCF (Skp1-..
  15. ANDROGEN RECEPTOR REGULATION OF REPRODUCTIVE FUNCTION
    Elizabeth Wilson; Fiscal Year: 2009
    ..Aim 2 pursues evidence for a phosphorylation-dependent interaction between MAGE-11 and the F-box protein Skp2 (S phase kinase-associated protein 2) to test our hypothesis that MAGE-11 functions as a subunit of the SCF (Skp1-..
  16. Role of Skp2 and Skp2B in breast cancer
    Doris Germain; Fiscal Year: 2009
    ..This application focuses on the role of a new isoform of the SCF ubiquitin ligase F-box protein Skp2, termed Skp2B, which we recently identified, in the regulation of mammary tumorigenesis...
  17. Regulation of the cell cycle by SCF and APC/C ubiquitin ligases
    Michele Pagano; Fiscal Year: 2009
    ..number of F-box proteins, only three human SCF ubiquitin ligases (containing the F-box proteins betaTrcp, Fbw7 and Skp2, respectively) have well-established substrates, many of which are involved in cell cycle control (e.g...
  18. HTLV-I Tax activates the anaphase promoting complex
    Chou Zen Giam; Fiscal Year: 2009
    ..Importantly, this senescence phenotype can be explained by the unscheduled activation of APC[Cdc20] and the loss of Skp2 (most likely APC-mediated), the substrate-recognition subunit of SCF[Skp2], a distinct E3 ubiquitin ligase that ..
  19. Novel Substrates of SCF Ubiquitin Ligases in Cell Cycle Control and Cancer
    Michele Pagano; Fiscal Year: 2007
    ..Notably, only three out of 68 human SCF ubiquitin ligases (containing the F-box proteins UTrcp, Fbw7 and Skp2, respectively) have well-established substrates, many of which are involved in cell cycle control...
  20. GROWTH GENES, EXTRACELLULAR MATRIX AND ANGIOGENESIS
    Donald Ingber; Fiscal Year: 2007
    ..to the cell cycle machinery through activation of its downstream effector mDia and modulation of the F-box protein Skp2 that targets p27 for degradation...
  21. Regulation of Skp2 Expression in Urinary Bladder Wall
    STEVEN WEINTRAUB; Fiscal Year: 2005
    ..We found that when there is a bladder outlet obstruction, expression of the F-box protein Skp2 is upregulated in the tissue of the bladder wall...
  22. Interplay between Cdh1 and major regulatory pathways in human cancer
    Wenyi Wei; Fiscal Year: 2010
    ..We previously reported that Cdh1 targets the Skp2 oncoprotein for proteolysis;our preliminary data showed that the PI3K/Akt pathway protected Skp2 from Cdh1-mediated ..
  23. Interplay between Cdh1 and major regulatory pathways in human cancer
    Wenyi Wei; Fiscal Year: 2009
    ..We previously reported that Cdh1 targets the Skp2 oncoprotein for proteolysis; our preliminary data showed that the PI3K/Akt pathway protected Skp2 from Cdh1-..
  24. Skp2 in androgen-dependent proliferation of prostate cancer cells
    Liang Zhu; Fiscal Year: 2010
    ..Our hypothesis to be tested in this application is that the F-box protein Skp2 is an important downstream effector of AR in mediating proliferation of AD and some AI prostate cancer cells ..
  25. Cell cycle in development and regeneration of the inner ear
    Neil Segil; Fiscal Year: 2011
    ..In spite of the importance of p27Klp1 transcriptional regulation, we have observed that in Skp2 mutant mice, there is also a defect in cell cycle exit and organ of Corti structure...
  26. Cell cycle in development and regeneration of the inner ear
    Neil Segil; Fiscal Year: 2010
    ..In spite of the importance of p27Klp1 transcriptional regulation, we have observed that in Skp2 mutant mice, there is also a defect in cell cycle exit and organ of Corti structure...