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| phospholambanSummaryGene Symbol: phospholamban Description: phospholamban Alias: CMD1P, CMH18, PLB, cardiac phospholamban Species: human Top Publications
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Publications
Common variants at ten loci influence QT interval duration in the QTGEN StudyChristopher Newton-Cheh
Center for Human Genetic Research, Cardiovascular Research Center, Massachusetts General Hospital, Boston, MA, USA
Nat Genet 41:399-406. 2009..5% of the variation in QT interval. These results, together with an accompanying paper, offer insights into myocardial repolarization and suggest candidate genes that could predispose to sudden cardiac death and drug-induced arrhythmias...
Common variants at ten loci modulate the QT interval duration in the QTSCD StudyArne Pfeufer
Institute of Human Genetics, Helmholtz Center Munich, Germany
Nat Genet 41:407-14. 2009..These results, together with an accompanying paper from the QTGEN consortium, identify new candidate genes for ventricular arrhythmias and SCD...
A human phospholamban promoter polymorphism in dilated cardiomyopathy alters transcriptional regulation by glucocorticoidsKobra Haghighi
Department of Pharmacology and Cell Biophysics, University of Cincinnati, College of Medicine, Cincinnati, Ohio 45267 0575, USA
Hum Mutat 29:640-7. 2008Depressed calcium handling by the sarcoplasmic reticulum (SR) Ca-ATPase and its regulator phospholamban (PLN) is a key characteristic of human and experimental heart failure...
Phospholamban R14 deletion results in late-onset, mild, hereditary dilated cardiomyopathyMegan M DeWitt
Department to Medicine, Section of Cardiology, The University of Chicago, Chicago, Illinois, USA
J Am Coll Cardiol 48:1396-8. 2006The purpose of this research was to determine the phenotypic spectrum associated with phospholamban gene (PLN) mutations.
A mutation in the human phospholamban gene, deleting arginine 14, results in lethal, hereditary cardiomyopathyKobra Haghighi
Department of Pharmacology and Cell Biophysics, University of Cincinnati College of Medicine, Cincinnati, OH 45267, USA
Proc Natl Acad Sci U S A 103:1388-93. 2006..we identified a previously uncharacterized deletion of arginine 14 (PLN-R14Del) in the coding region of the phospholamban (PLN) gene in a large family with hereditary heart failure. No homozygous individuals were identified...
Genetic deletion of arginine 14 in phospholamban causes dilated cardiomyopathy with attenuated electrocardiographic R amplitudesMaximilian G Posch
Experimental and Clinical Research Center ECRC, Berlin, Germany
Heart Rhythm 6:480-6. 2009..Thus, identification of disease-causing mutations is a challenging and time-consuming task. Genotype-phenotype associations may alleviate identification of the underlying mutation...
Mutational screening of phospholamban gene in hypertrophic and idiopathic dilated cardiomyopathy and functional study of the PLN -42 C>G mutationMaria Medin
Hospital Universitario Juan Canalejo, Spain
Eur J Heart Fail 9:37-43. 2007b>Phospholamban is an endogenous sarcoplasmic reticulum calcium ATPase inhibitor with a regulatory effect on cardiac contraction/relaxation coupling...
Sarcolipin inhibits polymerization of phospholamban to induce superinhibition of sarco(endo)plasmic reticulum Ca2+-ATPases (SERCAs)Michio Asahi
Banting and Best Department of Medical Research, University of Toronto, Toronto, Ontario M5G 1L6, Canada
J Biol Chem 277:26725-8. 2002..of fast-twitch skeletal muscle (SERCA1a), is also expressed in cardiac and slow-twitch skeletal muscles where phospholamban (PLN) and SERCA2a are expressed...
Human phospholamban null results in lethal dilated cardiomyopathy revealing a critical difference between mouse and humanKobra Haghighi
Department of Pharmacology and Cell Biophysics, University of Cincinnati, College of Medicine, Cincinnati, Ohio 45267, USA
J Clin Invest 111:869-76. 2003..In mice, disruption of the PLN gene encoding phospholamban (PLN) or expression of dominant-negative PLN mutants enhances SR and cardiac function, but effects of PLN ..
Dilated cardiomyopathy and heart failure caused by a mutation in phospholambanJoachim P Schmitt
Department of Genetics, Harvard Medical School and Howard Hughes Medical Institute, 200 Longwood Avenue, Boston, MA 02115, USA
Science 299:1410-3. 2003..congestive heart failure is caused by a dominant Arg --> Cys missense mutation at residue 9 (R9C) in phospholamban (PLN), a transmembrane phosphoprotein that inhibits the cardiac sarcoplasmic reticular Ca2+-adenosine ..
Sarcolipin regulates sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA) by binding to transmembrane helices alone or in association with phospholambanMichio Asahi
Banting and Best Department of Medical Research, University of Toronto, Toronto, ON, Canada M5G 1L6
Proc Natl Acad Sci U S A 100:5040-5. 2003b>Phospholamban (PLN), a regulator of sarco(endo)plasmic reticulum Ca(2+)-ATPases (SERCAs), interacts with both the cytosolic N domain and transmembrane helices M2, M4, M6, and M9 of SERCA...
Mutation of the phospholamban promoter associated with hypertrophic cardiomyopathySusumu Minamisawa
Department of Pediatric Cardiology, The Heart Institute of Japan, Tokyo Women s Medical University, 8 1 Kawada cho, Shinjuku ku, 162 8666, Tokyo, Japan
Biochem Biophys Res Commun 304:1-4. 2003b>Phospholamban is an endogenous inhibitor of sarcoplasmic reticulum calcium ATPase and plays a prime role in cardiac contractility and relaxation. Phospholamban may be a candidate gene responsible for cardiomyopathy...
Genetic screening of calcium regulation genes in familial hypertrophic cardiomyopathyChristine Chiu
Agnes Ginges Centre for Molecular Cardiology, Centenary Institute, Sydney Australia
J Mol Cell Cardiol 43:337-43. 2007..for mutations in the Ca(2+) regulatory genes, sorcin (SRI), calstabin (FKBP1B), calsequestrin (CASQ2), phospholamban (PLN), sarcolipin (SLN), calreticulin (CALR3) and calmodulin (CALM)...
Immunolocalization of fibroblast growth factor-1 (FGF-1), its receptor (FGFR-1), and fibroblast-specific protein-1 (FSP-1) in inflammatory renal diseaseMichele Rossini
Department of Pathology, Vanderbilt University Medical Center, Nashville, TN 37232, USA
Kidney Int 68:2621-8. 2005....
Kinetic analysis of covalent hybrid plasminogen activators: effect of CNBr-degraded fibrinogen on kinetic parameters of Glu1-plasminogen activationP P Lee
Department of Medicine, University of Chicago Medical Center/Michael Reese Hospital and Medical Center, Pritzker School of Medicine, University of Chicago, Illinois 60616
Biochemistry 27:7506-13. 1988..CNBr-Fg enhanced significantly the activities of only two activators, t-PA and PlnA-t-PAB...
Activation of CD4+ and CD8+ lymphocyte subsets by streptozotocin in the popliteal lymph node assay. II. Comparison with acute graft-vs-host reaction in H-2 incompatible F1 mouse hybridsK Krzystyniak
Laboratoire d'Immunotoxicologie, INSERM U80, , Lyon, France
Immunopharmacol Immunotoxicol 14:865-82. 1992..abstract truncated at 400 words)..
Purification and characterization of secretory phospholipase B, lysophospholipase and lysophospholipase/transacylase from a virulent strain of the pathogenic fungus Cryptococcus neoformansS C Chen
Centre for Infectious Diseases and Microbiology, University of Sydney, Westmead Hospital, Westmead, NSW 2145, Australia
Biochem J 347:431-9. 2000..A highly active extracellular phospholipase, demonstrating phospholipase B (PLB), lysophospholipase (LPL) and lysophospholipase/transacylase (LPTA) activities, was purified to homogeneity from C...
Reduced sarcoplasmic reticulum Ca2+ uptake and increased Na+-Ca2+ exchanger expression in left ventricle myocardium of dogs with progression of heart failureSudhish Mishra
Department of Medicine, Division of Cardiovascular Medicine, Henry Ford Heart and Vascular Institute, Henry Ford Hospital, Detroit, MI 48202, USA
Heart Vessels 20:23-32. 2005..These regulatory proteins include Ca(2+)-ATPase (SERCA 2A), phospholamban (PLB), ryanodine-sensitive Ca(2+) release channels (RR), and the sarcolemmal Na(+)-Ca(2+) exchanger (NCX)...
Differential expression of homing receptors and vascular addressins in tonsils and draining lymph nodes: Effect of Brucella infection in sheepVanessa Suraud
UR 1282, Unité d Infectiologie Animale et Santé Publique, Institut National de Recherche Agronomique, Centre de Tours Nouzilly, F 37380 Nouzilly, France
Vet Immunol Immunopathol 115:239-50. 2007....
Immunomodulatory effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin tested by the popliteal lymph node assayF Fan
Department of Pharmacology, Toxicology and Therapeutics, University of Kansas Medical Center, Kansas City 66160 7417, USA
Toxicol Pathol 23:513-7. 1995..Results also suggest that drugs may be useful surrogates to study the mechanism of toxicity of environmental chemicals that cannot be administered to humans...
Phosphorylation of phospholamban by cAMP-dependent protein kinase enhances interactions between Ca-ATPase polypeptide chains in cardiac sarcoplasmic reticulum membranesS Negash
Department of Biochemistry, University of Kansas, Lawrence 66045 2106, USA
Biochemistry 35:11247-59. 1996..accompany the activation of the cardiac sarcoplasmic reticulum (SR) Ca-ATPase following the phosphorylation of phospholamban (PLB)...
Frequency-dependent acceleration of relaxation in mammalian heart: a property not relying on phospholamban and SERCA2a phosphorylationCarlos A Valverde
Centro de Investigaciones Cardiovasculares, Facultad de Ciencias Medicas, Universidad Nacional de La Plata, 60 y 120, 1900 La Plata, Argentina
J Physiol 562:801-13. 2005..is the Ca(2+)-calmodulin-dependent protein kinase (CaMKII)-dependent phosphorylation of the Thr(17) site of phospholamban (PLN)...
Identification of phospholipase B from Dictyostelium discoideum reveals a new lipase family present in mammals, flies and nematodes, but not yeastClive P Morgan
Department of Physiology, Rockefeller Building, University College London, University St, London WC1E 6JJ, UK
Biochem J 382:441-9. 2004..14, 253-257]. We assayed Dictyostelium lysates to demonstrate the presence of a highly active phospholipase B (PLB) enzyme that removed both fatty-acid chains from phosphatidylcholine and produced the water-soluble ..
Investigating the TNP-OVA and direct popliteal lymph node assays for the detection of immunostimulation by drugs associated with anaphylaxis in humansBradford W Gutting
Drug Safety Evaluation, Pfizer Global Research and Development, Groton Laboratories, Groton, CT 06340, USA
J Appl Toxicol 22:177-83. 2002....
Development of the natural response of immunoglobulin secreting cells in the pig as a function of organ, age and housingA T Bianchi
Department of Mammalian Virology, DLO Institute of Animal Science and Health, Lelystad, The Netherlands
Dev Comp Immunol 23:511-20. 1999....
Relative roles of pneumolysin and hydrogen peroxide from Streptococcus pneumoniae in inhibition of ependymal ciliary beat frequencyR A Hirst
Department of Child Health, University of Leicester, Leicester Royal Infirmary, Leicester LE2 7LX, United Kingdom
Infect Immun 68:1557-62. 2000..There is also a slight additive effect of pneumolysin and H(2)O(2) on ependymal toxicity; however, the precise mechanism of action and the role of these toxins in pathogenesis remain unclear...
Roles of CaM kinase II and phospholamban in SNP-induced relaxation of murine gastric fundus smooth musclesMinkyung Kim
Dept of Physiology and Cell Biology, Univ of Nevada School of Medicine, Anderson Bldg MS352, Reno, NV 89557, USA
Am J Physiol Cell Physiol 291:C337-47. 2006..Our findings that caffeine relaxes and hyperpolarizes murine gastric fundus smooth muscles and increases phospholamban (PLB) phosphorylation by Ca2+/calmodulin (CaM)-dependent protein kinase II (CaM kinase II) suggest that PLB ..
Viscum album agglutinin-I induces degradation of cytoskeletal proteins in leukaemia PLB-985 cells differentiated toward neutrophils: cleavage of non-muscle myosin heavy chain-IIA by caspasesValérie Lavastre
INRS Institut Armand Frappier, Universite du Quebec, Pointe Claire, QC, Canada
Br J Haematol 138:545-54. 2007The role of the anti-cancer agent Viscum album agglutinin-I (VAA-I) in leukaemia PLB-985 cells differentiated toward a neutrophil-like phenotype by dimethylsulphoxide (PLB-985D) has never been studied...
Reduced inhibitor 1 and 2 activity is associated with increased protein phosphatase type 1 activity in left ventricular myocardium of one-kidney, one-clip hypertensive ratsRamesh C Gupta
Cardiovascular Medicine, Department of Medicine, Henry Ford Heart and Vascular Institute, Henry Ford Health System, Detroit, MI 48202, USA
Mol Cell Biochem 269:49-57. 2005..activity and protein expression of PP1, INH-1 and INH-2 and phosphorylation of sarcoplasmic reticulum (SR) phospholamban (PLB), a substrate of PP1 and modulator of SR Ca2+-ATPase activity, in failing and non-failing hearts...
The homeostasis of phosphatidylcholine and lysophosphatidylcholine was not disrupted during tri-o-cresyl phosphate-induced delayed neurotoxicity in hensWei Yuan Hou
Laboratory of Molecular Toxicology, State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Datunlu Road, Beijing 100101, PR China
Toxicology 252:56-63. 2008..of OPIDN have recently been discovered that NTE acts as lysophospholipase (LysoPLA) in mice and phospholipase B (PLB) in cultured mammalian cells...
Defective Ca(2+) handling proteins regulation during heart failureS T Hu
Department of Biophysics, Second Military Medical University, Shanghai, People s Republic of China
Physiol Res 60:27-37. 2011..calmodulin, sorcin, calsequestrin, protein phosphatase 1, protein phosphatase 2A), Ca(2+) ATPase (SERCA2a), PLB phosphorylation at Ser16 (PLB-S16), PLB phosphorylation at Thr17 (PLB-T17), L-type Ca(2+) channel (Cav1...
Popliteal lymph node enlargement and antibody production in the mouse induced by zimeldine and related compounds with varying side chainsC Thomas
Research Institute of Toxicology, University of Utrecht, The Netherlands
Int J Immunopharmacol 12:561-8. 1990..The capacity of the compounds to induce PLN responses appeared not related to their pharmacological potential to inhibit the reuptake of serotonin and noradrenaline.(ABSTRACT TRUNCATED AT 250 WORDS)..
Role of dual-site phospholamban phosphorylation in the stunned heart: insights from phospholamban site-specific mutantsM Said
Centro de Investigaciones Cardiovasculares, Facultad de Ciencias Médicas 60 y 120, 1900 La Plata, Argentina
Am J Physiol Heart Circ Physiol 285:H1198-205. 2003Phosphorylation of phospholamban (PLB) at Ser16 (protein kinase A site) and at Thr17 [Ca2+/calmodulin kinase II (CaMKII) site] increases sarcoplasmic reticulum Ca2+ uptake and myocardial contractility and relaxation...
Structural requirements for hydantoins and 2-thiohydantoins to induce lymphoproliferative popliteal lymph node reactions in the mouseM E Kammuller
Department of Pharmacology, Pharmacy and Toxicology, Faculty of Veterinary Sciences, University of Utrecht, The Netherlands
Int J Immunopharmacol 10:997-1010. 1988..The PLN assay may therefore be useful as a preliminary short-term screening method for identification of (classes of) compounds able to induce lymphoproliferative reactions.(ABSTRACT TRUNCATED AT 400 WORDS)..
Comparable levels of Ca-ATPase inhibition by phospholamban in slow-twitch skeletal and cardiac sarcoplasmic reticulumDeborah A Ferrington
Department of Biochemistry, University of Kansas, Lawrence, Kansas 66045, USA
Biochemistry 41:13289-96. 2002Alterations in expression levels of phospholamban (PLB) relative to the sarcoplasmic reticulum (SR) Ca-ATPase have been suggested to underlie defects of calcium regulation in the failing heart and other cardiac pathologies...
Molecular karyotype variation in Leishmania (Viannia) peruviana: indication of geographical populations in Peru distributed along a north-south clineJ C Dujardin
Laboratory of Protozoology, Institute of Tropical Medicine, Prince Leopold, Antwerpen, Belgium
Ann Trop Med Parasitol 87:335-47. 1993..All the isolates could be distinguished from L. braziliensis by their pLb-134 hybridization patterns...
Phospholamban oligomerization, quaternary structure, and sarco(endo)plasmic reticulum calcium ATPase binding measured by fluorescence resonance energy transfer in living cellsEileen M Kelly
Department of Physiology, Loyola University Chicago, Maywood, Illinois 60153, USA
J Biol Chem 283:12202-11. 2008b>Phospholamban (PLB) oligomerization, quaternary structure, and sarco(endo)plasmic reticulum calcium ATPase (SERCA) binding were quantified by fluorescence resonance energy transfer (FRET) in an intact cellular environment...
[Effects of NADPH oxidase inhibition on cardiac function and myocardial calcium regulatory proteins in rabbits with heart failure]Yu Liu
Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan 430060, China
Zhonghua Xin Xue Guan Bing Za Zhi 37:883-6. 2009..To investigate the effects of NADPH oxidase inhibition on cardiac function and myocardial calcium regulatory proteins mRNA expressions in rabbits with heart failure (HF)...
Mouse popliteal lymph node assay for assessment of allergic and autoimmunity-inducing potentials of low-molecular-weight drugsK Shinkai
Japan Pharmaceutical Manufacturers Association, Tokyo, Japan
J Toxicol Sci 24:95-102. 1999....
Effects of simvastatin on cardiac performance and expression of sarcoplasmic reticular calcium regulatory proteins in rat heartXia Zheng
The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China
Acta Pharmacol Sin 26:696-704. 2005..function and the alteration of gene and protein expression of the sarcoplasmic calcium regulatory proteins, including sarcoplasmic reticulum Ca2+-ATPase (SERCA), phospholamban (PLB), and ryanodine receptor 2 (RyR2) in rat hearts.
Role of phospholamban in cyclic GMP mediated signaling in cardiac myocytesQihang Zhang
Heart and Brain Circulation Laboratory, Department of Physiology and Biophysics, UMDNJ Robert Wood Johnson Medical School, Piscataway, NJ 08854 8021, USA
Cell Physiol Biochem 20:157-66. 2007..the hypothesis that the negative functional effects of cyclic GMP on cardiac myocytes were mediated through phospholamban (PLB) and activation of sarcoplasmic reticulum Ca(2+)-ATPase...
[Anatomic and clinical study on 4-bone-tunnel double-bundle anterior cruciate ligament]Jia kuo Yu
Institute of Sports Medicine, Peking University Third Hospital, Beijing 100083, China
Zhonghua Yi Xue Za Zhi 89:2019-24. 2009..To conduct a primary clinical analysis on 411 cases of arthroscopic 4 bone-tunnel double-bundle anterior cruciate ligament (ACL) reconstruction done by one operator on the basis of anatomic measurements of cadaver knees...
Applicability of the popliteal lymph node assay in the Brown-Norway ratF Verdier
Laboratoire d'Immunotoxicologie Fondamentale et Clinique, INSERM U80-CNRS URA 1177-UCBL, , Lyon, France
Immunopharmacol Immunotoxicol 12:669-77. 1990..This first set of experiments indicates that the PLN assay is applicable to Brown-Norway rats and warrants further investigation...
Effects of imposed pursed-lips breathing on respiratory mechanics and dyspnea at rest and during exercise in COPDJadranka Spahija
School of Physical and Occupational Therapy, McGill University, Montreal, QC, Canada
Chest 128:640-50. 2005To investigate the effect of volitional pursed-lips breathing (PLB) on breathing pattern, respiratory mechanics, operational lung volumes, and dyspnea in patients with COPD.
Phospholamban modulates the functional coupling between nucleotide domains in Ca-ATPase oligomeric complexes in cardiac sarcoplasmic reticulumLinda T L Chen
Biological Sciences Division, Pacific Northwest National Laboratory, P O Box 999, Richland, Washington 99352, USA
Biochemistry 48:2411-21. 2009Oligomeric interactions between Ca-ATPase polypeptide chains and their modulation by phospholamban (PLB) were measured in native cardiac sarcoplasmic reticulum (SR) microsomes...
Cytosolic phospholipase A2 is responsible for prostaglandin E2 and leukotriene B4 formation in phagocyte-like PLB-985 cells: studies of differentiated cPLA2-deficient PLB-985 cellsI Furstenberg Liberty
Department of Clinical Biochemistry, Faculty of Health Sciences, Ben Gurion University of the Negev, Beer Sheva 84105, Israel
J Leukoc Biol 76:176-84. 2004Our previously established model of cytosolic phospholipase A(2) (cPLA(2))-deficient, differentiated PLB-985 cells (PLB-D cells) was used to determine the physiological role of cPLA(2) in eicosanoid production...
The homeostasis of phosphatidylcholine and lysophosphatidylcholine in nervous tissues of mice was not disrupted after administration of tri-o-cresyl phosphateWei Yuan Hou
Laboratory of Molecular Toxicology, State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, PR China
Toxicol Sci 109:276-85. 2009Neuropathy target esterase (NTE) is proven to act as a lysophospholipase (LysoPLA) in mice and phospholipase B (PLB) in cultured mammalian cells...
Familial protein-losing enteropathy and protein-losing nephropathy in Soft Coated Wheaten Terriers: 222 cases (1983-1997)M P Littman
Department of Clinical Studies, School of Veterinary Medicine, University of Pennsylvania, Philadelphia 19104 6010, USA
J Vet Intern Med 14:68-80. 2000..The prognosis is poor. Compared with previously reported intestinal and renal diseases in dogs, a new, distinctive familial predisposition for both PLE and PLN has been recognized in the SCWT breed...
Competing irradiation techniques for para-aortic lymph nodes: dose distribution and NTCP for the kidneyMeinhard Nevinny-Stickel
Department of Therapeutic Radiology and Oncology, Innsbruck Medical University, Innsbruck, Austria
Int J Radiat Oncol Biol Phys 63:1206-13. 2005....
Effects of CMAP and electrostatic cutoffs on the dynamics of an integral membrane protein: the phospholamban studyY Houndonougbo
Department of Chemistry, Biochemistry and Institute of Biomedical Studies, Baylor University, 101 Bagby Ave, Waco, TX 76706, USA
J Biomol Struct Dyn 26:17-34. 2008In our effort to understand the microscopic structure and dynamics of phospholamban (PLB), a small integral membrane protein, we have performed a series of 5-20 ns molecular dynamics simulations to explore the influence of environment (..
Effects on recovery during acidosis in cardiac myocytes overexpressing CaMKIICan M Sag
Department of Cardiology and Pneumology Heart Center, Georg August University Gottingen, Robert Koch Strasse 40, 37075 Gottingen, Germany
J Mol Cell Cardiol 43:696-709. 2007..shortening during late phase acidosis are suggested to be associated with CaMKII-dependent processes of which phospholamban (PLB) phosphorylation may play an important role...
Lusitropic effects of dobutamine in young and aged mice in vivoEmily D Hirleman
Sarver Heart Center, College of Medicine, The University of Arizona, Tucson, Arizona 85724, USA
J Extra Corpor Technol 40:10-5. 2008..elements by sarcoplasmic reticulum (SR) Ca(2+)-ATPase (SERCA2a), which is regulated by an inhibitory protein, phospholamban (PLB)...
Role of leucine 31 of phospholamban in structural and functional interactions with the Ca2+ pump of cardiac sarcoplasmic reticulumZhenhui Chen
Krannert Institute of Cardiology and the Department of Medicine, Indiana University School of Medicine, 1800 N Capitol Ave, Indianapolis, Indiana 46202, USA
J Biol Chem 280:10530-9. 2005The ability of two loss-of-function mutants, L31A and L31C, of phospholamban (PLB) to bind to and inhibit the Ca(2+) pump of cardiac sarcoplasmic reticulum (SERCA2a) was investigated using a molecular cross-linking approach...
Increased particulate partitioning of PKC epsilon reverses susceptibility of phospholamban knockout hearts to ischemic injuryKimberly N Gregory
Department of Pharmacology and Cell Biophysics, University of Cincinnati College of Medicine, 231 Albert Sabin Way, Cincinnati, OH 45267 0575, USA
J Mol Cell Cardiol 36:313-8. 2004..of sarcoplasmic reticulum Ca(2+) cycling through inhibition or ablation of the Ca(2+) ATP-ase inhibitor phospholamban (PLN), which shows promise as a treatment for heart failure, could be beneficial in ischemic heart disease...
Peptide inhibitors use two related mechanisms to alter the apparent calcium affinity of the sarcoplasmic reticulum calcium pumpMichael R Afara
Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada T6G 2H7
Biochemistry 47:9522-30. 2008The primary sequence of phospholamban (PLB) has provided a template for the rational design of peptide inhibitors of the sarcoplasmic reticulum calcium ATPase (SERCA)...
Femoral bone strength and its relation to cortical and trabecular changes after treatment with PTH, alendronate, and their combination as assessed by finite element analysis of quantitative CT scansTony M Keaveny
Department of Mechanical Engineering, University of California, Berkeley, California 94720 1740, USA
J Bone Miner Res 23:1974-82. 2008..were assigned to either PTH, ALN, or their combination (CMB) in year 1 and were switched to either ALN or placebo (PLB) treatment in year 2: PTH-PLB, PTH-ALN, CMB-ALN, and ALN-ALN (year 1-year 2) treatments...
Depressed contractile reserve and impaired calcium handling of cardiac myocytes from chronically unloaded hearts are ameliorated with the administration of physiological treatment dose of T3 in ratsY Minatoya
Department of Cardiovascular Medicine, Tohoku University Graduate School of Medicine, Sendai, Japan
Acta Physiol (Oxf) 189:221-31. 2007Chronic cardiac unloading causes a time-dependent upregulation of phospholamban (PLB) and depression of myocyte contractility in normal rat hearts...
Chemoattractant mechanism of Th1 cells in class III and IV lupus nephritisShinji Morimoto
Department of Internal Medicine and Rheumatology, Juntendo University School of Medicine, Tokyo, Japan
Autoimmunity 42:143-9. 2009..These findings suggest that combination of Th1 inducers and chemokine inhibition might be powerful threrapeutic approach in PLN...
Phospholamban ablation rescues sarcoplasmic reticulum Ca(2+) handling but exacerbates cardiac dysfunction in CaMKIIdelta(C) transgenic miceTong Zhang
Department of Pharmacology, University of California, San Diego, 9500 Gilman Dr, La Jolla, CA 92093 0636, USA
Circ Res 106:354-62. 2010..We hypothesized that phospholamban (PLN) ablation would restore SR Ca(2+) load and prevent the decreased ventricular contractility, dilation and ..
Potential risks of femoral tunnel drilling through the far anteromedial portal: a cadaveric studyMitsuhiro Nakamura
Department of Orthopaedic Surgery, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan
Arthroscopy 25:481-7. 2009..The purpose of this study was to estimate the potential risks when drilling femoral tunnels through the far anteromedial portal in double-bundle anterior cruciate ligament reconstruction in cadaveric knees...
Perlecan knockdown in metastatic prostate cancer cells reduces heparin-binding growth factor responses in vitro and tumor growth in vivoCristiana Savorè
Department of Biological Sciences, University of Delaware, Newark, DE 19716, USA
Clin Exp Metastasis 22:377-90. 2005..We conclude that Pln is an essential ECM component involved in growth responses of metastatic prostate cancer cells to HBGFs deposited in local and metastatic microenvironment...
[Comparison of cardiac function and expression and activity of myocardial calcium regulatory proteins in rabbit systolic and diastolic heart failure models.]Lei Wang
Department of Cardiology, The First Affiliated Hospital of Soochow University, Suzhou 215006, China Department of Laboratory Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China E mail
Sheng Li Xue Bao 61:551-8. 2009..The mRNA expression levels of sarcoplasmic reticulum Ca(2+) ATPase 2a (SERCA2a) and phospholamban (PLB) were evaluated by RT-PCR...
Elevated levels of endogenous adenosine alter metabolism and enhance reduction in contractile function during low-flow ischemia: associated changes in expression of Ca(2+)-ATPase and phospholambanH T Sommerschild
Institute for Experimental Medical Research, Ulleval Hospital, Oslo, Norway
J Mol Cell Cardiol 31:1897-911. 1999..catecholamine-induced increase in contractile function mainly through inhibition of phosphorylation of phospholamban (PLB), the main regulatory protein of Ca(2+)-ATPase in sarcoplasmic reticulum (SR), and during ischemia it ..
Popliteal lymph node reactions in mice induced by the drug zimeldineC Thomas
Department of Toxicology, University of Utrecht, The Netherlands
Int J Immunopharmacol 11:693-702. 1989..The protracted nature of the reaction may indicate that zimeldine somehow interferes with inhibitory feedback mechanisms...
Expression of slow skeletal troponin I in hearts of phospholamban knockout mice alters the relaxant effect of beta-adrenergic stimulationBeata M Wolska
Department of Medicine, Section of Cardiology, University of Illinois at Chicago, Chicago, Ill 60612 7323, USA
Circ Res 90:882-8. 2002..in an enhanced relaxation rate in association with phosphorylation of both cardiac troponin I (cTnI) and phospholamban (PLB)...
Vasodilator-stimulated phosphoprotein regulates inside-out signaling of beta2 integrins in neutrophilsRavi K Deevi
Centre for Infection and Immunity, Queen s University Belfast, Belfast, Northern Ireland, United Kingdom
J Immunol 184:6575-84. 2010..found that fMLP-induced activation of Rap1 in human polymorphonuclear leukocytes or neutrophils and differentiated PLB-985 cells was blocked by inhibitors of the NO/guanosine-3',5'-cyclic monophosphate-dependent protein kinase (cGKI) ..
Identification of functional domains of rat intestinal phospholipase B/lipase. Its cDNA cloning, expression, and tissue distributionH Takemori
Department of Molecular Physiological Chemistry, Osaka University Medical School, Japan
J Biol Chem 273:2222-31. 1998A cDNA encoding a rat intestinal Ca(2+)-independent phospholipase B/lipase (PLB/LIP) was cloned from an ileac mucosa cDNA library using a probe amplified by polymerase chain reaction based on the purified enzyme's sequence...
Lymphocyte populations and adhesion molecule expression in bovine tonsilsM C Rebelatto
Department of Veterinary Pathobiology, School of Veterinary Medicine, Purdue University, West Lafayette, IN 47907 1243, USA
Vet Immunol Immunopathol 73:15-29. 2000..Similarities between the human and bovine tonsils suggest that cattle may provide a good model to study the role of the tonsil in the respiratory immune response...
Mechanism of reversal of phospholamban inhibition of the cardiac Ca2+-ATPase by protein kinase A and by anti-phospholamban monoclonal antibody 2D12Zhenhui Chen
Krannert Institute of Cardiology and the Department of Medicine, Indiana University School of Medicine, Indianapolis, Indiana 46202, USA
J Biol Chem 282:20968-76. 2007Our model of phospholamban (PLB) regulation of the cardiac Ca(2+)-ATPase in sarcoplasmic reticulum (SERCA2a) states that PLB binds to the Ca(2+)-free, E2 conformation of SERCA2a and blocks it from transitioning from E2 to E1, the Ca(2+)-..
Fine ambient particles from various sites in europe exerted a greater IgE adjuvant effect than coarse ambient particles in a mouse modelTorunn Alberg
Norwegian Institute of Public Health, Division of Environmental Medicine, Oslo, Norway
J Toxicol Environ Health A 72:1-13. 2009....
Early over expression of messenger RNA for multiple genes, including insulin, in the Pancreatic Lymph Nodes of NOD mice is associated with Islet AutoimmunityBéatrice Regnault
Immunophysiology and Intracellular Parasitism Unit, Department of Parasitology and Mycology, 75015 Paris, France
BMC Med Genomics 2:63. 2009..Analysis of gene expression profiles has the potential to provide a global understanding of the disease and to generate novel hypothesis concerning the initiation of the autoimmune process...
Probing excited states and activation energy for the integral membrane protein phospholamban by NMR CPMG relaxation dispersion experimentsNathaniel J Traaseth
Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, MN 55445, USA
Biochim Biophys Acta 1798:77-81. 2010b>Phospholamban (PLN) is a dynamic single-pass membrane protein that inhibits the flow of Ca(2+) ions into the sarcoplasmic reticulum (SR) of heart muscle by directly binding to and inhibiting the SR Ca(2+)ATPase (SERCA)...
Radiologic evaluation of the insertion sites of the 2 functional bundles of the anterior cruciate ligament using 3-dimensional computed tomographyStephan Lorenz
Department of Orthopaedic Sports Medicine, Klinikum rechts der Isar, Technical University of Munich, Munich, Germany
Am J Sports Med 37:2368-76. 2009..Reference values for the anatomic insertions are necessary for radiographic intra- and postoperative control and fluoroscopy-based navigation...
Description of the attachment geometry of the anteromedial and posterolateral bundles of the ACL from arthroscopic perspective for anatomical tunnel placementJoan W H Luites
Research, Development and Education, OrthoResearch Unit, Sint Maartenskliniek, P O Box 9011, 6500 GM, Nijmegen, The Netherlands
Knee Surg Sports Traumatol Arthrosc 15:1422-31. 2007The anterior cruciate ligament (ACL) consists of an anteromedial bundle (AMB) and a posterolateral bundle (PLB)...
Solution structure of the cytoplasmic domain of phopholamban: phosphorylation leads to a local perturbation in secondary structureR J Mortishire-Smith
Neuroscience Research Centre, Merck Sharp and Dohme Research Laboratories, Harlow, Essex, United Kingdom
Biochemistry 34:7603-13. 1995Peptides representing the N-terminal domain (Ia) of the cardiac sarcoplasmic reticulum protein phospholamban (residues 1-25 [PLB(1-25)] and a phosphorylated form [pPLB(1-25)]) were synthesized and their conformations examined using ..
Chronic human immunodeficiency virus type 1 infection stimulates distinct NF-kappa B/rel DNA binding activities in myelomonoblastic cellsA Roulston
Lady Davis Institute for Medical Research, Sir Mortimer B Davis Jewish General Hospital, Montreal, Quebec, Canada
J Virol 67:5235-46. 1993..induction of NF-kappa B binding activity was examined in a myeloid cell model of HIV-1 infection derived from the PLB-985 cell line...
Influence of prolactin and growth hormone on the activation of dwarf mouse lymphocytes in vivoR R Gala
Department of Physiology, Wayne State University School of Medicine, Detroit, Michigan 48201
Proc Soc Exp Biol Med 204:224-30. 1993..These findings strongly suggest that PRL and/or GH play an important role at some stage of the T cell activation process in vivo. Further studies are needed to precisely identify the defect in the dwarf mice...
Identification of the druggable concavity in homology models using the PLB indexShinji Soga
Molecular Medicine Research Laboratories, Drug Discovery Research, Astellas Pharma Inc, 21 Miyukigaoka, Tsukuba, Ibaraki 305 8585, Japan
J Chem Inf Model 47:2287-92. 2007..We previously proposed an index named PLB (propensity for ligand binding), which is based on the amino acid composition characteristically observed at the ..
Low-dose intramuscular polymyxin B improves survival of septic ratsT Mayumi
Department of Emergency Medicine, Nagoya University School of Medicine, Japan
Shock 11:82-6. 1999Polymyxin B (PLB) is a cationic antibiotic that also stoichiometrically neutralizes the lipid A moiety of endotoxin...
Förster transfer recovery reveals that phospholamban exchanges slowly from pentamers but rapidly from the SERCA regulatory complexSeth L Robia
Department of Physiology, 102 5609, Loyola University Chicago, 2160 South First Ave, Maywood, IL 60153, USA
Circ Res 101:1123-9. 2007b>Phospholamban (PLB) or the sarcoplasmic reticulum Ca2+-ATPase (SERCA) were fused to cyan fluorescent protein (CFP) and coexpressed with PLB fused to yellow fluorescent protein (YFP)...
Differential effects of phospholamban and Ca2+/calmodulin-dependent kinase II on [Ca2+]i transients in cardiac myocytes at physiological stimulation frequenciesAndreas A Werdich
Department of Biomedical Engineering, Vanderbilt University, 6301 Stevenson Center, VU Station B 351631, Nashville, TN 37235 1631, USA
Am J Physiol Heart Circ Physiol 294:H2352-62. 2008..from and Ca(2+) uptake into the sarcoplasmic reticulum via the phosphorylation of the ryanodine receptor 2 and phospholamban (PLN), respectively...
Regulation of sarcoplasmic reticulum Ca2+ reuptake in porcine airway smooth muscleVenkatachalem Sathish
Department of Physiology and Biomedical Engineering, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
Am J Physiol Lung Cell Mol Physiol 294:L787-96. 2008..We examined regulation of SERCA in porcine ASM: our hypothesis was that the regulatory protein phospholamban (PLN) and the calmodulin (CaM)-CaM kinase (CaMKII) pathway (both of which are known to regulate SERCA in ..
Targeted ablation of the phospholamban gene is associated with a marked decrease in sensitivity in aortic smooth muscleJ Lalli
University of Cincinnati, College of Medicine, Department of Molecular and Cellular Physiology, OH 45267 0576, USA
Circ Res 80:506-13. 1997b>Phospholamban (PLB) is a protein associated with the Ca(2+)-ATPase of the sarcoplasmic reticulum (SR) in cardiac, slow-twitch skeletal, and smooth muscle...
Solid-state (2)H and (15)N NMR studies of side-chain and backbone dynamics of phospholamban in lipid bilayers: investigation of the N27A mutationShidong Chu
Department of Chemistry and Biochemistry, Miami University, Oxford, OH 45056, USA
Biochim Biophys Acta 1798:210-5. 2010b>Phospholamban (PLB) is an integral membrane protein regulating Ca(2+) transport through inhibitory interaction with sarco(endo)plasmic reticulum calcium ATPase (SERCA)...
[Construction of lentiviral vector carrying human VE-cadherin gene and expression of VE-cadherin in leukemic cell line Sup-B15]Huan Xin Zhang
Laboratory of Transplantation Immunology, Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China
Zhongguo Shi Yan Xue Ye Xue Za Zhi 19:574-7. 2011..The VE-cadherin DNA fragment was subcloned into pLB vector to generate a lentiviral vector pLB-VEC...
The relationship between different spectral forms of the protochlorophyllide oxidoreductase complex and the structural organisation of prolamellar bodies isolated from Zea maysEva Selstam
Umea Plant Science Centre, Department of Plant Physiology, University of Umea, Umea, Sweden
Photosynth Res 108:47-59. 2011Incubation of prolamellar bodies (PLB) in high-salt media leads to changes in PLB structure and properties of their protochlorophyllide oxidoreductase-protochlorophyllide (POR-PChlide) complex...
Efficacy of aripiprazole adjunctive to lithium or valproate in the long-term treatment of patients with bipolar I disorder with an inadequate response to lithium or valproate monotherapy: a multicenter, double-blind, randomized studyRonald Marcus
Bristol Myers Squibb, 5 Research Parkway, Wallingford, CT 06492, USA
Bipolar Disord 13:133-44. 2011..safety of aripiprazole (ARI) adjunctive to lithium (Li) or valproate (Val) (ARI+Li?/?Val) compared with placebo (PLB) adjunctive to Li or Val (PLB+Li?/?Val) as maintenance therapy for patients with bipolar I disorder who had an ..
New dendronized polymers from acrylate Behera amine and their ability to produce visco-elastic structured fluids when mixed with CTAT worm-like micellesJulio C Cuggino
Departamento de Quimica Organica, Facultad de Ciencias Quimicas, Universidad Nacional de Cordoba, Cordoba, Argentina
J Colloid Interface Sci 357:147-56. 2011..However, such synergistic viscosity enhancement saturated at rather low concentrations (25ppm) indicating an apparent lower solubility as compared to PL1, a fact that may be related to its higher molecular weight...
SERCA2a superinhibition by human phospholamban triggers electrical and structural remodeling in mouse heartsHong Sheng Wang
Department of Pharmacology, College of Medicine, University of Cincinnati, Cincinnati, Ohio 45267 0575, USA
Physiol Genomics 43:357-64. 2011b>Phospholamban (PLN), the reversible inhibitor of the sarco(endo)plasmic reticulum Ca(2+)-ATPase (SERCA2a), is a key regulator of myocyte Ca(2+) cycling with a significant role in heart failure...
Does pelvic lymph nodes irradiation using intensity modulated radiation therapy increase rectal and bladder toxicities in patients with prostate carcinoma?M Garipagaoglu
Acibadem University, Department of Radiation Oncology, Istanbul, Turkey
J BUON 15:668-73. 2010....
PLN-encoded phospholamban mutation in a large cohort of hypertrophic cardiomyopathy cases: summary of the literature and implications for genetic testingAndrew P Landstrom
Mayo Medical School, Mayo Clinic, Rochester, MN 55905, USA
Am Heart J 161:165-71. 2011..We sought to determine the spectrum and prevalence of mutations in PLN-encoded phospholamban in a large cohort of HCM cases as a potential cause of mutation-negative HCM.
Clinical characterization and prognostic factors of primary lymphoma of bone in case of Chinese patientsJia Jia Huang
State Key Laboratory of Oncology in Southern China, Department of Medical Oncology, Cancer Center, Sun Yat Sen University, Guangzhou, Guangdong, China
Med Oncol 28:S476-82. 2011Primary lymphoma of bone (PLB) is a very rare neoplasm. Many aspects of this entity have not been well defined or reported in Chinese population. The purpose of our study is to identify the prognostic factors of PLB...
Factors discriminating spontaneous pursed-lips breathing use in patients with COPDJadranka Spahija
McGill University, Montreal, Quebec, Canada
COPD 7:254-61. 2010Pursed-lips breathing (PLB) is often spontaneously performed by chronic obstructive pulmonary disease (COPD) patients. The aim of this study was to evaluate spontaneous PLB prevalence and to identify factors discriminating its use...
Inhibition of phospholamban phosphorylation by O-GlcNAcylation: implications for diabetic cardiomyopathyShunichi Yokoe
Department of Biochemistry, Hyogo College of Medicine, 1 1 Mukogawa cho, Nishinomiya, Hyogo, Japan
Glycobiology 20:1217-26. 2010..b>Phospholamban (PLN) regulates the function of SERCA2a via its Ser(16)-phosphorylation...
A novel custom resequencing array for dilated cardiomyopathyRebekah S Zimmerman
Laboratory for Molecular Medicine, Partners Healthcare Center for Personalized Genetic Medicine, Cambridge, Massachusetts 02139, USA
Genet Med 12:268-78. 2010..Novel sequencing platforms have now opened up avenues for more comprehensive diagnostic testing while simultaneously decreasing test cost and turn around time...
Afa/Dr-expressing, diffusely adhering Escherichia coli strain C1845 triggers F1845 fimbria-dependent phosphatidylserine externalization on neutrophil-like differentiated PLB-985 cells through an apoptosis-independent mechanismNicolas Sémiramoth
INSERM, UMR756 Signalisation et Physiopathologie des Cellules Epithéliales, and Universite Paris Sud XI, Faculte de Pharmacie, Chatenay Malabry, France
Infect Immun 78:2974-83. 2010..Using the human PLB-985 cell line differentiated into fully mature neutrophils, we found that the wild-type enterovirulent E...
Real-time PCR expression profiling of genes encoding potential virulence factors in Candida albicans biofilms: identification of model-dependent and -independent gene expressionHeleen Nailis
Laboratory for Pharmaceutical Microbiology, Universiteit Gent, Harelbekestraat 72, B 9000, Ghent, Belgium
BMC Microbiol 10:114. 2010..wall protein) and of genes belonging to the ALS (agglutinin-like sequence), SAP (secreted aspartyl protease), PLB (phospholipase B) and LIP (lipase) gene families is associated with biofilm growth on mucosal surfaces...
Repression of cardiac phospholamban gene expression is mediated by thyroid hormone receptor-{alpha}1 and involves targeted covalent histone modificationsMadesh Belakavadi
Department of Physiology and Biophysics, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway, New Jersey 08854, USA
Endocrinology 151:2946-56. 2010b>Phospholamban (PLB) is a critical regulator of Ca(2+) cycling in heart muscle cells, and its gene expression is markedly down-regulated by T(3)...
(15)N Solid-state NMR spectroscopic studies on phospholamban at its phosphorylated form at ser-16 in aligned phospholipid bilayersShidong Chu
Department of Chemistry and Biochemistry, Miami University, Oxford, Ohio 45056, USA
Biochim Biophys Acta 1798:312-7. 2010Wild-type phospholamban (WT-PLB) is a pentameric transmembrane protein that regulates the cardiac cycle (contraction and relaxation)...
Ca2+ binding to site I of the cardiac Ca2+ pump is sufficient to dissociate phospholambanZhenhui Chen
Department of Medicine, Indiana University School of Medicine, Krannert Institute of Cardiology, Indianapolis, Indiana 46202, USA
J Biol Chem 285:3253-60. 2010b>Phospholamban (PLB) inhibits the activity of SERCA2a, the Ca(2+)-ATPase in cardiac sarcoplasmic reticulum, by decreasing the apparent affinity of the enzyme for Ca(2+)...
Activating and deactivating roles of lipid bilayers on the Ca(2+)-ATPase/phospholamban complexMartin Gustavsson
Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, Minnesota 55455, United States
Biochemistry 50:10367-74. 2011..the sarcoplasmic reticulum (SR) Ca(2+)-ATPase (SERCA) in the presence and absence of its endogenous regulator, phospholamban (PLN)...
Research Grants
- Characterizing autoimmunity in NOD mouse islets, PLN and spleenDaniel Kaufman; Fiscal Year: 2007..The results will help guide our thinking on potential therapeutic approaches, as well as the utility of biomarkers based on peripheral T cell responses. ..
- Cardiac Sarcoplasmic Reticulum Calcium Cycling ProteinsEvangelia G Kranias; Fiscal Year: 2010..SR Ca-uptake during diastole is mediated by SERCA2a and its reversible regulator phospholamban (PLN);Ca is stored by calsequestrin;and Ca is released during systole through the ryanodine receptor and its ..
- Calcium Handling in Cardiac PathophysiologyEvangelia Kranias; Fiscal Year: 2009..The major functions of the SR are: Ca-uptake mediated by SERCA2a and its reversible regulator phospholamban (PLN); Ca-storage through calsequestrin and the histidine rich Ca-binding protein (HRC); and Ca-release ..
- Calcium Handling in Cardiac PathophysiologyEvangelia G Kranias; Fiscal Year: 2010..The major functions of the SR are: Ca-uptake mediated by SERCA2a and its reversible regulator phospholamban (PLN);Ca-storage through calsequestrin and the histidine rich Ca-binding protein (HRC);and Ca-release ..
- Mechanisms regulating SR Ca2+ ATPase in the AtriaMuthu Periasamy; Fiscal Year: 2010..The activity of SERCA pump is regulated by two small molecular weight proteins, phospholamban (PLB) and sarcolipin (SLN)...
- AKAP Regulation of PKA Targeting in the HeartMeredith Bond; Fiscal Year: 2009..We also demonstrated that phosphorylation of Rll, and PKA substrates (Tnl, MBP-C and PLB), is decreased in failing human hearts...
- Phospholemman and Na-Pump Function in Cardiac MyocytesDonald M Bers; Fiscal Year: 2010..During the initial award period, we showed that PLM regulates NKA similar to the way phospholamban (PLB) regulates SR Ca-ATPase (SERCA)...
- Troponin and Heart Function in Health and DiseaseJOSEPH MARK METZGER; Fiscal Year: 2010..Therefore the health relevance of the proposal is substantial and highly significant to the mission of the National Institutes of Health. ..
- NMR Structural Analysis of Calcium Regulation in MuscleGianluigi Veglia; Fiscal Year: 2010..SERCA's function is regulated by two membrane inhibitors: phospholamban (PLN) and sarcolipin (PLN), expressed primarily in cardiac and skeletal muscle, respectively...
- RECEPTOR TOPOGRAPHY IN MICROVASCULAR LEUKOCYTE ADHESIONUlrich von Andrian; Fiscal Year: 2005..This may lead to a better understanding of physiologic and pathologic phenomena in PLN and result in improved vaccination protocols against tumors, infections and inflammatory diseases. ..
- Cardiac Sarcoplasmic Reticulum Calcium Cycling ProteinsEvangelia Kranias; Fiscal Year: 2009..The main SR proteins responsible for these functions are: the Ca-transport ATPase (SERCA2) with its regulator phospholamban (PLN); the Ca-storage proteins, calsequestrin and a histidine rich Ca-binding protein (HRC); and the Ca-..
- Cardiac Sarcoplasmic Reticulum Calcium Cycling ProteinsEvangelia Kranias; Fiscal Year: 2007..The main SR proteins responsible for these functions are: the Ca-transport ATPase (SERCA2) with its regulator phospholamban (PLN); the Ca-storage proteins, calsequestrin and a histidine rich Ca-binding protein (HRC); and the Ca-..
- CaMKII in Sinus Node Physiology and DiseaseMark Anderson; Fiscal Year: 2009..kinase II (CaMKII) is a serine- threonine kinase that regulates the SR-associated Ca2+ homeostatic proteins, phospholamban (PLN) and ryanodine receptors (RyR)...
- CaMKII in Sinus Node Physiology and DiseaseMark E Anderson; Fiscal Year: 2010..kinase II (CaMKII) is a serine- threonine kinase that regulates the SR-associated Ca2+ homeostatic proteins, phospholamban (PLN) and ryanodine receptors (RyR)...
- DIABETIC CARDIOMYOPATHY AND CALCIUM FLUXWolfgang Dillmann; Fiscal Year: 2003..sarcoplasmic reticulum (SR) calcium loading, calcium spark patterns and NA+/Ca exchanger activity and the phospholamban (Plb) phosphorylation status...
- Deaf1 isoforms control changes in PTA expression in the NOD PLN during T1D pathogCHARLES GARRISON FATHMAN; Fiscal Year: 2010..The studies proposed here will examine the role of Deaf1 in maintaining peripheral tolerance and help us understand how a change in Deaf1 splicing may contribute to the pathogenesis of T1D. ..
- Structural Dynamics of Muscle Calcium ATPase RegulationDAVID DALE THOMAS; Fiscal Year: 2010..SERCA), the large integral membrane enzyme that pumps calcium into the SR and thus relaxes the muscle, and on phospholamban (PLB), the small integral membrane protein that regulates SERCA in the heart...
- T Cell Reactivity in Human Pancreatic Draining Lymph Nodes in Type 1 DiabetesDavid Hafler; Fiscal Year: 2003..These investigations are designed to provide novel targets and approaches for the treatment of human autoimmune disease. ..
- BIOPHYSICAL STUDIES OF MEMBRANE MOLECULAR DYNAMICSDavid Thomas; Fiscal Year: 2007..SERCA), the large integral membrane enzyme that pumps calcium into the SR and thus relaxes the muscle, and phospholamban (PLB) and sarcolipin (SLN), the small integral membrane proteins that regulate SERCA...
- BIOPHYSICAL STUDIES OF MEMBRANE MOLECULAR DYNAMICSDavid Thomas; Fiscal Year: 2003..on the Ca-ATPase, the integral membrane enzyme that pumps calcium into the SR and thus relaxes the muscle, and phospholamban (PLB), the integral membrane peptide involved in regulating the Ca-ATPase in the heart...
- T Cell Activation in Lymph nodesUlrich H von Andrian; Fiscal Year: 2010..This information may lead to improved strategies for clinical immunomodulation, e.g. for vaccinations, tumor therapy and treatment of infectious, inflammatory and autoimmune diseases. ..
- T Cell Activation in Lymph nodesUlrich von Andrian; Fiscal Year: 2009..This information may lead to improved strategies for clinical immunomodulation, e.g. for vaccinations, tumor therapy and treatment of infectious, inflammatory and autoimmune diseases. ..
- T Cell Activation in Lymph nodesUlrich von Andrian; Fiscal Year: 2007..This information may lead to improved strategies for clinical immunomodulation, e.g. for vaccinations, tumor therapy and treatment of infectious, inflammatory and autoimmune diseases. ..
- ClC-3 ion transport modulates NADPH oxidase-dependent PMN priming by endotoxinJessica G Moreland; Fiscal Year: 2010..Whole cell patch clamp analysis of human and murine PMNs along with differentiated PLB cells will be employed to directly observe the relevant conductances, and will allow the use of molecular tools to ..
- Gene Therapy with Cardiotropic Vectors for the Treatment of Heart FailureROGER JOSEPH HAJJAR; Fiscal Year: 2010..with a decrease in the activity of the SR Ca2+-ATPase (SERCA2a), which is at least partially due to enhanced phospholamban (PLN) inhibition...
- Gene Therapy with Cardiotropic Vectors for the Treatment of Heart FailureROGER JOSEPH HAJJAR; Fiscal Year: 2011..with a decrease in the activity of the SR Ca2+-ATPase (SERCA2a), which is at least partially due to enhanced phospholamban (PLN) inhibition...
- PHOSPHOLIPASE B--MECHANISMS AUGMENTING CANDIDA VIRULENCEMahmoud Ghannoum; Fiscal Year: 2003..on exploiting the concept of candidal virulence by identifying and characterizing factors, such as phospholipase B (PLB), that augment the pathogenicity of this important nosocomial pathogen...
- B cells and draining lymph node function in arthritic flairEdward Schwarz; Fiscal Year: 2009..Here we propose to test this theory in mouse models and RA patients. ..
- Dissection of cellular interactions in T1DM with integrated functional genomicsMartin Hessner; Fiscal Year: 2009....
- Dissection of cellular interactions in T1DM with integrated functional genomicsMARTIN JOHN HESSNER; Fiscal Year: 2010....
- Dissecting Dendritic Cell Function in Autoimmune DiabetesJonathan Katz; Fiscal Year: 2009..Aim 2: To determine if NKT cell-produced INF-9 and pDC-produced IDO establish a regulatory circuit that controls diabetogenic T cells in vivo. ..
- Type 1 diabetes: the role of commensal microbiotaAlexander V Chervonsky; Fiscal Year: 2010....
- Dissecting Dendritic Cell Function in Autoimmune DiabetesJONATHAN DAVID KATZ; Fiscal Year: 2010..Aim 2: To determine if NKT cell-produced INF-9 and pDC-produced IDO establish a regulatory circuit that controls diabetogenic T cells in vivo. ..
- CARDIAC SARCOPLASMIC RETICULIM CALCIUM CYCLING PROTEINSEvangelia Kranias; Fiscal Year: 2004..b>Phospholamban is another SR protein, which plays a crucial role in the regulation of the Ca2+-ATPase activity and myocardial ..
- The role of syndecan-4 in the regulation of JNK activation in human neutrophilsPATRICK ARNDT; Fiscal Year: 2007..chemical inhibitors, blocking antibodies, small interfering RNAs, and protein transduction of neutrophils and the PLB-985 cell line, which can be differentiated into neutrophil-like cells...
