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| NEIL2SummaryGene Symbol: NEIL2 Description: nei endonuclease VIII-like 2 (E. coli) Alias: NEH2, NEI2, DNA glycosylase/AP lyase Neil2, DNA-(apurinic or apyrimidinic site) lyase Neil2, endonuclease 8-like 2, endonuclease VIII-like 2, nei homolog 2, nei like 2, nei-like protein 2 Species: human Top Publications
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Acetylation of the human DNA glycosylase NEIL2 and inhibition of its activityKishor K Bhakat
Sealy Center for Molecular Science and Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston, TX 77555 1079, USA
Nucleic Acids Res 32:3033-9. 2004..coactivator p300 stably interacts with, and acetylates, the recently discovered human DNA glycosylase NEIL2, involved in the repair of oxidized bases both in vivo and in vitro...- Functional variants of the NEIL1 and NEIL2 genes and risk and progression of squamous cell carcinoma of the oral cavity and oropharynxXiadong Zhai
Department of Epidemiology, The University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
Clin Cancer Res 14:4345-52. 2008Human DNA glycosylases NEIL1 and NEIL2 participate in oxidized base excision repair and protect cells from DNA damage... - Stimulation of NEIL2-mediated oxidized base excision repair via YB-1 interaction during oxidative stressSoumita Das
Sealy Center for Molecular Medicine and Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, Texas 77555, USA
J Biol Chem 282:28474-84. 2007The recently characterized enzyme NEIL2 (Nei-like-2), one of the four oxidized base-specific DNA glycosylases (OGG1, NTH1, NEIL1, and NEIL2) in mammalian cells, has poor base excision activity from duplex DNA... - AP endonuclease-independent DNA base excision repair in human cellsLee Wiederhold
Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston 77555, USA
Mol Cell 15:209-20. 2004..The recently discovered mammalian DNA glycosylase/AP lyases, NEIL1 and NEIL2, unlike the previously characterized OGG1 and NTH1, generate DNA strand breaks with 3' phosphate termini... - Deficiency of the oxidative damage-specific DNA glycosylase NEIL1 leads to reduced germinal center B cell expansionHiromi Mori
Laboratory for Immune Diversity, Research Center for Allergy and Immunology, RIKEN Yokohama Institute, 1 7 22 Suehiro cho, Tsurumi, Yokohama 230 0045, Japan
DNA Repair (Amst) 8:1328-32. 2009Mammalian cells possess multiple DNA glycosylases, including OGG1, NTH1, NEIL1, NEIL2 and NEIL3, for the repair of oxidative DNA damage... - Nucleotide, cytogenetic and expression impact of the human chromosome 8p23.1 inversion polymorphismNina Bosch
Genetic Causes of Disease Group, Genes and Disease Programme Center for Genomic Regulation CRG UPF, Barcelona, Catalonia, Spain
PLoS ONE 4:e8269. 2009..The identification of single nucleotide polymorphisms (SNPs) tightly linked to the genomic orientation of a given region should be useful to indirectly evaluate the genotypes of large genomic orientations in the individuals... - Discovery of the negative regulator of Nrf2, Keap1: a historical overviewKen Itoh
Department of Stress Response Science, Hirosaki University Graduate School of Medicine, Japan
Antioxid Redox Signal 13:1665-78. 2010..Keap1 suppresses Nrf2 activity by specifically binding to its evolutionarily conserved N-terminal Neh2 regulatory domain... - Structural analysis of the complex of Keap1 with a prothymosin alpha peptideBalasundaram Padmanabhan
Genomic Sciences Center, Yokohama Institute, RIKEN, 1 7 22 Suehiro cho, Tsurumi, Yokohama 230 0045, Japan
Acta Crystallogr Sect F Struct Biol Cryst Commun 64:233-8. 2008..which is formed by the double-glycine repeat and C-terminal region domains (Keap1-DC), interacts directly with the Neh2 domain of Nrf2... - Identification of the interactive interface and phylogenic conservation of the Nrf2-Keap1 systemMakoto Kobayashi
The Center for Tsukuba Advanced Research Alliance and Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba 305 8577, Japan
Genes Cells 7:807-20. 2002..To gain an insight into our present enigma as to how cells receive oxidative and electrophilic signals and transduce them to Nrf2, we have developed a zebrafish model system for molecular toxicological studies... - Cul3-mediated Nrf2 ubiquitination and antioxidant response element (ARE) activation are dependent on the partial molar volume at position 151 of Keap1Aimee L Eggler
Center for Pharmaceutical Biotechnology and the Department of Medicinal Chemistry and Pharmacognosy, University of Illinois, Chicago, IL 60607, USA
Biochem J 422:171-80. 2009..This model has significant implications for how electrophiles which modify Cys(151), disrupt the repressive function of Keap1... - Base excision repair activities in organotypic hippocampal slice cultures exposed to oxygen and glucose deprivationVeslemøy Rolseth
Centre for Molecular Biology and Neuroscience, Institute of Medical Microbiology, University of Oslo, Rikshospitalet, N 0027 Oslo, Norway
DNA Repair (Amst) 7:869-78. 2008..Real-time RT-PCR showed that transcription of DNA glycosylases, including Ogg1, Nth1, Ung, Aag, Neil1 and Neil2 were not changed in response to OGD treatment (t=0 h)... - Human Nei-like protein NEIL3 has AP lyase activity specific for single-stranded DNA and confers oxidative stress resistance in Escherichia coli mutantMasashi Takao
Department of Molecular Genetics, Institute of Development, Aging and Cancer, Tohoku University, Aoba ku, Sendai, Japan
Genes Cells 14:261-70. 2009..damage to DNA, cells contain several DNA glycosylases including OGG1, NTH1 and the Nei-like proteins, NEIL1 and NEIL2. A third Nei-like protein, NEIL3, is composed of an amino-terminal Nei-like domain and an unknown carboxy-terminal .. - Expression and purification of NEIL3, a human DNA glycosylase homologSilje Z Krokeide
Centre for Molecular Biology and Neuroscience, Department of Molecular Biology, Institute of Microbiology, Rikshospitalet Medical Centre, Sognsvannsveien 28, N0027 Oslo, Norway
Protein Expr Purif 65:160-4. 2009..Thus, a mammalian family of three proteins (NEIL1, NEIL2 and NEIL3) that showed homology to the Escherichia coli Fpg/Nei DNA glycosylases was identified... - A simple method for amino acid selective isotope labeling of recombinant proteins in E. coliKit I Tong
Center for Tsukuba Advanced Research Alliance, Graduate School of Comprehensive Human Sciences, JST ERATO Environmental Response Project, University of Tsukuba, Tsukuba, 305 8577, Japan
J Biomol NMR 42:59-67. 2008..The sequential backbone resonance assignment of the Neh2 domain of Nrf2 transcriptional factor, an intrinsically disordered protein with high spectral degeneracy, was .. - Polynucleotide kinase as a potential target for enhancing cytotoxicity by ionizing radiation and topoisomerase I inhibitorsN K Bernstein
Department of Biochemistry, University of Alberta, 11560 University Avenue, Edmonton, Alberta, Canada
Anticancer Agents Med Chem 8:358-67. 2008..agents including ionizing radiation, topoisomerase I inhibitors, and DNA repair glycosylases such as NEIL1 and NEIL2, often contain 5'-hydroxyl and/or 3'-phosphate termini... - Structural insights into the similar modes of Nrf2 transcription factor recognition by the cytoplasmic repressor Keap1Balasundaram Padmanabhan
Genomic Sciences Center, Yokohama Institute, RIKEN, 1 7 22 Suehiro cho, Tsurumi, Yokohama 230 0045, Japan
J Synchrotron Radiat 15:273-6. 2008..The Neh2 domain of Nrf2 interacts with Keap1 at the bottom region of the Kelch/beta-propeller domain which is formed by .. - Prospective type 1 and type 2 disulfides of Keap1 proteinRyan Holland
Department of Chemistry and Biochemistry, University of Maryland Baltimore County, 1000 Hilltop Circle, Baltimore, Maryland 21250, USA
Chem Res Toxicol 21:2051-60. 2008..The energy minimizations indicated marked alterations in structure with a substantial constriction of Neh2 binding domain of the Keap1 Kelch repeat domain... - Novel DNA glycosylases from Mycobacterium tuberculosisV S Sidorenko
Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk 630090, Russia
Biochemistry (Mosc) 73:442-50. 2008..We have cloned and expressed two paralogs (Mtu-Nei2 and Mtu-Fpg2) from 36KAZ and KHA94 isolates of Mycobacterium tuberculosis and studied their ability to participate .. - Widespread distribution of DNA glycosylases removing oxidative DNA lesions in human and rodent brainsVeslemøy Rolseth
Centre for Molecular Biology and Neuroscience, Institute of Medical Microbiology, University of Oslo, Rikshospitalet HF, Oslo, Norway
DNA Repair (Amst) 7:1578-88. 2008..In mammalian cells at least five different DNA glycosylases with overlapping substrate specificity, NEIL1, NEIL2, NEIL3, OGG1 and NTH1, remove oxidative DNA base lesions... - Mutator phenotype of mammalian cells due to deficiency of NEIL1 DNA glycosylase, an oxidized base-specific repair enzymeAmit K Maiti
Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX 77555, USA
DNA Repair (Amst) 7:1213-20. 2008The recently characterized NEIL1 and NEIL2 are distinct from the previously characterized mammalian DNA glycosylases (OGG1 and NTH1) involved in repair of oxidized bases because of the NEILs' preference for excising base lesions from .. - Single nucleotide polymorphisms 5' upstream the coding region of the NEIL2 gene influence gene transcription levels and alter levels of genetic damageCarla J Kinslow
Department of Preventive Medicine and Community Health, University of Texas Medical Branch, Galveston, TX 77555 1110, USA
Genes Chromosomes Cancer 47:923-32. 2008b>NEIL2 (EC 4.2.99.18), a mammalian DNA glycosylase and ortholog of the bacterial Fpg/Nei, excises oxidized DNA lesions from bubble or single-stranded structures, suggesting its involvement in transcription-coupled DNA repair... - Heavy metal ions affect the activity of DNA glycosylases of the fpg familyI R Grin
Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, pr Lavrentieva 8, Novosibirsk, Russia
Biochemistry (Mosc) 74:1253-9. 2009..enzymes formamidopyrimidine-DNA glycosylase (Fpg) and endonuclease VIII (Nei) and their eukaryotic homologs NEIL1, NEIL2, and NEIL3 define the Fpg family of DNA glycosylases, which initiate the process of repair of oxidized DNA bases... - Signaling pathways activated by the phytochemical nordihydroguaiaretic acid contribute to a Keap1-independent regulation of Nrf2 stability: Role of glycogen synthase kinase-3Ana I Rojo
Departamento de Bioquímica e Instituto de Investigaciones Biomédicas Alberto Sols UAM CSIC, Centro de Investigación en Red sobre Enfermedades Neurodegenerativas, Instituto de Investigación Sanitaria La Paz, Madrid 28029, Spain
Free Radic Biol Med 52:473-87. 2012..The stability of Nrf2 involves the interaction of two degradation domains, designated Neh2 and Neh6, with the E3 ubiquitin ligase adaptors, Keap1 and ?-TrCP, respectively... - Peptide inhibitors of the Keap1-Nrf2 protein-protein interactionRowena Hancock
Department of Pharmaceutical and Biological Chemistry, School of Pharmacy, London WC1N 1AX, UK
Free Radic Biol Med 52:444-51. 2012..We describe the structure-activity relationships for a series of peptides based upon regions of the Nrf2 Neh2 domain, of varying length and sequence, that interact with the Keap1 Kelch domain and disrupt the interaction with .. - DNA base excision repair gene polymorphisms modulate human cognitive performance and decline during normal life spanMeryl S Lillenes
Centre for Molecular Biology and Neuroscience, Institute of Microbiology, University of Oslo, Norway
Mech Ageing Dev 132:449-58. 2011..MutYH(His324Gln), APE1(Gln51His), APE1(Glu148Asp), XRCC1(Lys298Asn), XRCC1(Arg7Leu), NEIL1(Asp252Asn), and NEIL2(Arg257Leu). XRCC1(Arg7Leu) and PolB(Pro242Arg) were characterized by single nucleotide variations (?0... - Development of Neh2-luciferase reporter and its application for high throughput screening and real-time monitoring of Nrf2 activatorsNatalya A Smirnova
Burke Medical Research Institute, Department of Neurology and Neuroscience, Weill Medical College of Cornell University, White Plains, NY 10605, USA
Chem Biol 18:752-65. 2011..To directly monitor stabilization of Nrf2, we fused its Neh2 domain, responsible for the interaction with its nucleocytoplasmic regulator, Keap1, to firefly luciferase (Neh2-.. - Eukaryotic endonuclease VIII-like proteins: new components of the base excision DNA repair systemI R Grin
Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia
Biochemistry (Mosc) 76:80-93. 2011..In 2002, three new human DNA glycosylases (NEIL1, NEIL2, and NEIL3) were discovered, all homologous to endonuclease VIII, a bacterial protein, which also participates in .. - Duffy-null-associated low neutrophil counts influence HIV-1 susceptibility in high-risk South African black womenVeron Ramsuran
HIV Pathogenesis Programme, Doris Duke Medical Research Institute, Nelson R Mandela School of Medicine, University of KwaZulu Natal, Durban, South Africa
Clin Infect Dis 52:1248-56. 2011..The influence of pre-seroconversion levels of peripheral blood cell counts (PBCs) on the risk of acquiring human immunodeficiency virus (HIV)-1 infection among Africans is unknown... - Integrative analysis of array-comparative genomic hybridisation and matched gene expression profiling data reveals novel genes with prognostic significance in oesophageal adenocarcinomaX Y Goh
MRC Cancer Cell Unit, Hutchison MRC Research Centre, Cambridge, UK
Gut 60:1317-26. 2011..This study aimed to use genome-wide genomic and expression data to enhance the understanding of OAC pathogenesis and identify groups with differential outcomes... - Molecular mechanisms of the Keap1–Nrf2 pathway in stress response and cancer evolutionKeiko Taguchi
Department of Medical Biochemistry, Tohoku University Graduate School of Medicine, 2 1 Seiryo cho, Aoba ku, Sendai 980 8575, Japan
Genes Cells 16:123-40. 2011..in which one molecule of Nrf2 associates with two molecules of Keap1 by using two binding sites within the Neh2 domain of Nrf2. This two-site binding appears critical for Nrf2 ubiquitination... - Induction of NEIL1 and NEIL2 DNA glycosylases in aniline-induced splenic toxicityHuaxian Ma
Department of Pathology, University of Texas Medical Branch, Galveston, TX 77555, USA
Toxicol Appl Pharmacol 251:1-7. 2011..of NEIL1/2 was assayed using a bubble structure substrate containing 5-OHU (preferred substrates for NEIL1 and NEIL2) and by quantitating the cleavage products. Aniline treatment led to a 1... - Specific Inhibition of NEIL-initiated repair of oxidized base damage in human genome by copper and iron: potential etiological linkage to neurodegenerative diseasesMuralidhar L Hegde
Department of Biochemistry, University of Texas Medical Branch, Galveston, Texas 77555, USA
J Biol Chem 285:28812-25. 2010..repair pathway, initiated with one of four major DNA glycosylases: NTH1 or OGG1 (of the Nth family) or NEIL1 or NEIL2 (of the Nei family)... - The mouse ortholog of NEIL3 is a functional DNA glycosylase in vitro and in vivoMinmin Liu
Department of Microbiology and Molecular Genetics, The Markey Center for Molecular Genetics, University of Vermont, Stafford Hall, 95 Carrigan Drive, Burlington, VT 05405 0086, USA
Proc Natl Acad Sci U S A 107:4925-30. 2010..have been identified in mammals that are homologous to the Escherichia coli Fpg and Nei proteins, Neil1, Neil2, and Neil3... - Inactivation of NEIL2 DNA glycosylase by pyridoxal phosphate reveals a loop important for substrate bindingInga R Grin
SB RAS Institute of Chemical Biology and Fundamental Medicine, 8 Lavrentieva Ave, Novosibirsk 630090, Russia
Biochem Biophys Res Commun 394:100-5. 2010..Of six enzymes tested, only endonuclease VIII-like protein 2 (NEIL2) was significantly inhibited by PLP. The inhibition was due to Schiff base formation between PLP and the enzyme... - Hepatitis C virus induces oxidative stress, DNA damage and modulates the DNA repair enzyme NEIL1Sampa Pal
Department of Laboratory Medicine, University of Washington, Seattle, Washington 98195 7110 USA
J Gastroenterol Hepatol 25:627-34. 2010..In the current study we examined the induction of reactive oxygen species (ROS) resulting from HCV infection and evaluated its effect on the host DNA damage and repair machinery... - Regulatory regions responsive to oxidative stress in the promoter of the human DNA glycosylase gene NEIL2Carla J Kinslow
Department of Obstetrics and Gynecology, The University of Texas Medical Branch, Galveston, TX 77555 1062, USA
Mutagenesis 25:171-7. 2010..If not repaired, these lesions could lead to genomic instability and, potentially, to cancer development. NEIL2 (EC 4.2.99... - REG4, NEIL2, and BIRC5 gene expression correlates with gamma-radiation sensitivity in patients with rectal cancer receiving radiotherapyTakashi Kobunai
Department of Surgery, Teikyo University School of Medicine, 2 11 1 Kaga, Itabashi ku, Tokyo 173 8605, Japan
Anticancer Res 31:4147-53. 2011..The present study aimed to identify genes that influence the susceptibility of cancer cells to radiation... - Interaction of the human DNA glycosylase NEIL1 with proliferating cell nuclear antigen. The potential for replication-associated repair of oxidized bases in mammalian genomesHong Dou
Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, Texas 77555 1079, USA
J Biol Chem 283:3130-40. 2008NEIL1 and NEIL2 compose a family of DNA glycosylases that is distinct from that of the other two DNA glycosylases, OGG1 and NTH1, all of which are involved in repair of oxidized bases in mammalian genomes... - Keap1 is a redox-regulated substrate adaptor protein for a Cul3-dependent ubiquitin ligase complexDonna D Zhang
Department of Biochemistry, University of Missouri Columbia, M121 Medical Sciences Building, Columbia, MO 65212, USA
Mol Cell Biol 24:10941-53. 2004..E3 ubiquitin ligase complex with Cul3 and Rbx1 that targets multiple lysine residues located in the N-terminal Neh2 domain of Nrf2 for ubiquitin conjugation both in vivo and in vitro... - Recognition of the oxidized lesions spiroiminodihydantoin and guanidinohydantoin in DNA by the mammalian base excision repair glycosylases NEIL1 and NEIL2M Katie Hailer
Department of Chemistry, The University of Montana, Missoula, MT 59812, USA
DNA Repair (Amst) 4:41-50. 2005..Herein we report that two recently identified mammalian glycosylases, NEIL1 and NEIL2, showed a high affinity for recognition and cleavage of DNA containing Gh/Ia and Sp lesions... - Identification of a zinc finger domain in the human NEIL2 (Nei-like-2) proteinAditi Das
Sealy Center for Molecular Science and Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston, Texas 77555 1079, USA
J Biol Chem 279:47132-8. 2004The recently identified human NEIL2 (Nei-like-2) protein, a DNA glycosylase/AP lyase specific for oxidatively damaged bases, shares structural features and reaction mechanism with the Escherichia coli DNA glycosylases, Nei and Fpg... - Redox-regulated turnover of Nrf2 is determined by at least two separate protein domains, the redox-sensitive Neh2 degron and the redox-insensitive Neh6 degronMichael McMahon
Biomedical Research Centre, Ninewells Hospital and Medical School, University of Dundee, Dundee DD1 9SY, Scotland, United Kingdom
J Biol Chem 279:31556-67. 2004..In homeostatic COS1 cells, the Neh2 degron confers on Nrf2 a half-life of less than 10 min... - Differential specificity of human and Escherichia coli endonuclease III and VIII homologues for oxidative base lesionsAtsushi Katafuchi
Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Higashi Hiroshima 739 8526, Japan
J Biol Chem 279:14464-71. 2004..However, detailed analysis of the cellular activity suggests that hNEIL1 has a significant role in the repair of 5S-Tg in human cells... - Repair of oxidized bases in DNA bubble structures by human DNA glycosylases NEIL1 and NEIL2Hong Dou
Sealy Center for Molecular Science and Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston, Texas 77555, USA
J Biol Chem 278:49679-84. 2003..The newly discovered human DNA glycosylases, NEIL1 and NEIL2, are distinct in structural features and reaction mechanism from the previously characterized NTH1 and OGG1 but .. - Phosphorylation of Nrf2 at Ser40 by protein kinase C in response to antioxidants leads to the release of Nrf2 from INrf2, but is not required for Nrf2 stabilization/accumulation in the nucleus and transcriptional activation of antioxidant response elementDavid A Bloom
Department of Pharmacology, Baylor College of Medicine, Houston, Texas 77030, USA
J Biol Chem 278:44675-82. 2003..The PKC site at Ser40 is conserved among species and lies in the Neh2 domain, which interacts with INrf2... - The novel DNA glycosylase, NEIL1, protects mammalian cells from radiation-mediated cell deathThomas A Rosenquist
Department of Pharmacological Sciences, SUNY Stony Brook, Stony Brook, NY 11794, USA
DNA Repair (Amst) 2:581-91. 2003..Three human genes, designated NEIL1, NEIL2 and NEIL3, encode proteins that contain sequence homologies to Nei and Fpg... - Keap1-dependent proteasomal degradation of transcription factor Nrf2 contributes to the negative regulation of antioxidant response element-driven gene expressionMichael McMahon
Biomedical Research Centre, Ninewells Hospital and Medical School, University of Dundee, Dundee DD1 9SY, Scotland, United Kingdom
J Biol Chem 278:21592-600. 2003..Within Nrf2, the N-terminal Neh2 domain is identified as the redox-sensitive degron... - A novel human DNA glycosylase that removes oxidative DNA damage and is homologous to Escherichia coli endonuclease VIIIViswanath Bandaru
Department of Microbiology and Molecular Genetics, The Markey Center for Molecular Genetics, The University of Vermont, Stafford Hall, 95 Carrigan Drive, Burlington, VT 05405 0068, USA
DNA Repair (Amst) 1:517-29. 2002..Furthermore, inactivation of active site residues shown to be important in Escherichia coli Nei inactivate the human enzyme. The hNEI1 gene is located on the long arm of chromosome 15 that is frequently deleted in human cancers... - A back-up glycosylase in Nth1 knock-out mice is a functional Nei (endonuclease VIII) homologueMasashi Takao
Department of Molecular Genetics, Institute of Development, Aging and Cancer, Tohoku University, Sendai 980 8575, Japan
J Biol Chem 277:42205-13. 2002..These results suggest that NEIL1 is a back-up glycosylase for NTH1 with unique substrate specificity and tissue-specific expression... - Direct evidence that sulfhydryl groups of Keap1 are the sensors regulating induction of phase 2 enzymes that protect against carcinogens and oxidantsAlbena T Dinkova-Kostova
The Lewis B and Dorothy Cullman Cancer Chemoprotection Center, Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Proc Natl Acad Sci U S A 99:11908-13. 2002..and purified murine Keap1 and demonstrated on native gels the formation of complexes of Keap1 with the Neh2 domain of Nrf2 and their concentration-dependent disruption by inducers such as sulforaphane and bis(2-.. - Identification and characterization of a novel human DNA glycosylase for repair of cytosine-derived lesionsTapas K Hazra
Department of Human Biological Chemistry and Genetics, Sealy Center for Molecular Science, University of Texas Medical Branch, 6 136 Medical Research Building, Rte 1079, Galveston, TX 77555, USA
J Biol Chem 277:30417-20. 2002..A. 99, 3523-3528). Here we report characterization of the second protein, originally named NEH2 and now renamed NEIL2 (Nei-like). The 37-kDa wild-type NEIL2 expressed in and purified from E... - The murine DNA glycosylase NEIL2 (mNEIL2) and human DNA polymerase beta bind microtubules in situ and in vitroKimberly A Conlon
Department of Pharmacological Sciences, School of Medicine University Hospital and Medical Center, State University of New York Stony Brook, NY 11794 8651, USA
DNA Repair (Amst) 4:419-31. 2005.... - Modifying specific cysteines of the electrophile-sensing human Keap1 protein is insufficient to disrupt binding to the Nrf2 domain Neh2Aimee L Eggler
Center for Pharmaceutical Biotechnology, University of Illinois, Chicago, IL 60607, USA
Proc Natl Acad Sci U S A 102:10070-5. 2005..Under basal conditions, Keap1 sequesters Nrf2 in the cytoplasm by binding to its Neh2 domain... - Differential modulation of base excision repair activities during brain ontogeny: implications for repair of transcribed DNAElla W Englander
Department of Surgery, The University of Texas Medical Branch, Shriners Hospitals for Children, 815 Market Street, Galveston, TX 77550, USA
Mech Ageing Dev 127:64-9. 2006..While a subset of BER enzymes, exhibited declining expression and excision activities, expression of NEIL1 and NEIL2 glycosylases increased during brain development... - Subcellular localization and cytoplasmic complex status of endogenous Keap1Yoriko Watai
Graduate School of Comprehensive Human Sciences, Center for Tsukuba Advanced Research Alliance, University of Tsukuba, Tsukuba, Japan
Genes Cells 12:1163-78. 2007..an adaptor for Cullin-3-based ubiquitin ligase, and a regulator of Nrf2 activity through the interaction with Nrf2 Neh2 domain... - Different electrostatic potentials define ETGE and DLG motifs as hinge and latch in oxidative stress responseKit I Tong
Graduate School of Comprehensive Human Sciences, Center for TARA, JST ERATO Environmental Response Project, University of Tsukuba, 1 1 1 Tennoudai, Tsukuba 305 8577, Japan
Mol Cell Biol 27:7511-21. 2007..The DLG motif works to correctly position the lysines within the Nrf2 Neh2 domain for efficient ubiquitination... - Novel vectors for homologous recombination strategies in mouse embryonic stem cells: an ES cell line expressing EGFP under control of the 5T4 promoterFlor M Perez Campo
Stem Cell Biology Group, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Wilmslow Road, Manchester, UK
Exp Cell Res 313:3604-15. 2007..As proof-of-principle, we have utilized the knock-out targeting vector to modify the NEIL2 locus in ES cells... - Human non-synonymous single nucleotide polymorphisms can influence ubiquitin-mediated protein degradationSevtap Savas
Fred A Litwin Centre for Cancer Genetics, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
OMICS 11:200-8. 2007..in the presence of nsSNPs in CCND3, PMS2, POLE4, SITPEC, and PPARG and putative PEST motifs were created in NEIL2, BIRC4, MLL2, and PPP1R15A... - Human polymorphic variants of the NEIL1 DNA glycosylaseLaura M Roy
Center for Research on Occupational and Environmental Toxicology, Department of Molecular and Medical Genetics, Oregon Health and Science University, Portland, Oregon 97239 3098, USA
J Biol Chem 282:15790-8. 2007..reactive oxygen species is primarily initiated by a series of DNA glycosylases that include OGG1, NTH1, NEIL1, and NEIL2. To explore the functional significance of NEIL1, we recently reported that neil1 knock-out and heterozygotic mice .. - Different organization of base excision repair of uracil in DNA in nuclei and mitochondria and selective upregulation of mitochondrial uracil-DNA glycosylase after oxidative stressM Akbari
Department of Cancer Research and Molecular Medicine, Faculty of Medicine, Norwegian University of Science and Technology, N 7006 Trondheim, Norway
Neuroscience 145:1201-12. 2007..The DNA glycosylases Nei-like DNA glycosylase 1 (NEIL1), Nei-like DNA glycosylase 2 (NEIL2), mitochondrial uracil-DNA glycosylase 1 (UNG1), nuclear uracil-DNA glycosylase 2 (UNG2) and endonuclease III-like .. - Evaluation of NTHL1, NEIL1, NEIL2, MPG, TDG, UNG and SMUG1 genes in familial colorectal cancer predispositionPeter Broderick
Section of Cancer Genetics, Brookes Lawley Building, Institute of Cancer Research, 15 Cotswold Road, Sutton, Surrey, SM2 5NG, UK
BMC Cancer 6:243. 2006..It is conceivable that germline sequence variation in other BER pathway genes such as NTHL1, NEIL1, NEIL2, MPG, TDG, UNG and SMUG1 also contribute to CRC susceptibility. - NEIL2-initiated, APE-independent repair of oxidized bases in DNA: Evidence for a repair complex in human cellsAditi Das
Department of Biochemistry and Molecular Biology, Sealy Center for Molecular Science, University of Texas Medical Branch, 6 136 Medical Research Building, Route 1079, Galveston, TX 77555, USA
DNA Repair (Amst) 5:1439-48. 2006..barely active in removing 3' phosphate generated by the recently discovered mammalian DNA glycosylases NEIL1 and NEIL2. We showed earlier that the 3' phosphate generated by NEIL1 is efficiently removed by polynucleotide kinase (PNK) .. - Deoxyribophosphate lyase activity of mammalian endonuclease VIII-like proteinsInga R Grin
SB RAS Institute of Chemical Biology and Fundamental Medicine, Novosibirsk 630090, Russia
FEBS Lett 580:4916-22. 2006..Here, we demonstrate that NEIL1 and NEIL2, mammalian homologs of bacterial endonuclease VIII, excise dRP by beta-elimination with the efficiency similar to .. - Negative regulation of the Nrf1 transcription factor by its N-terminal domain is independent of Keap1: Nrf1, but not Nrf2, is targeted to the endoplasmic reticulumYiguo Zhang
Biomedical Research Centre, Ninewells Hospital and Medical School, University of Dundee, Dundee DD1 9SY, Scotland, UK
Biochem J 399:373-85. 2006..Within acidic domain 1, residues 156-242 share 43% identity with the Neh2 (Nrf2-ECH homology 2) degron of Nrf2 that serves to destabilize this latter transcription factor through an .. - Dimerization of substrate adaptors can facilitate cullin-mediated ubiquitylation of proteins by a "tethering" mechanism: a two-site interaction model for the Nrf2-Keap1 complexMichael McMahon
Biomedical Research Centre, Ninewells Hospital and Medical School, University of Dundee, Dundee DD1 9SY, Scotland, United Kingdom
J Biol Chem 281:24756-68. 2006..facilitates ubiquitylation of the Nrf2 transcription factor but only when this substrate possesses within its Neh2 domain a second cryptic Keap1-binding site, the DLG motif, in addition to its previously described ETGE site... - Keap1 recruits Neh2 through binding to ETGE and DLG motifs: characterization of the two-site molecular recognition modelKit I Tong
Graduate School of Life and Environmental Sciences, University of Tsukuba, 1 1 1 Tennoudai, Tsukuba 305 8572, Japan
Mol Cell Biol 26:2887-900. 2006..We found that the Neh2 domain of Nrf2 is an intrinsically disordered but biologically active regulatory domain containing a 33-residue .. - Keap1 represses nuclear activation of antioxidant responsive elements by Nrf2 through binding to the amino-terminal Neh2 domainK Itoh
Center for Tsukuba Advanced Research Alliance and Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba 305 8577, Japan
Genes Dev 13:76-86. 1999..The activation of Nrf2 leads in turn to the induction of phase II enzyme and antioxidative stress genes in response to electrophiles and reactive oxygen species... - Inherited predisposition to colorectal adenomas caused by multiple rare alleles of MUTYH but not OGG1, NUDT1, NTH1 or NEIL 1, 2 or 3A R Dallosso
Institute of Medical Genetics, School of Medicine, Cardiff University, Heath Park, Cardiff CF14 4XN, UK
Gut 57:1252-5. 2008..MUTYH functions in the DNA base excision repair pathway and has a key role in the repair of oxidative DNA damage...
Research Grants
- Repair of Oxidized Bases in Mammalian GenomesSankar Mitra; Fiscal Year: 2010..In our previous project period we characterized human glycosylases (NEIL1 and NEIL2) with overlapping substrate preferences as OGG1 and NTH1...
- Repair of Oxidized Bases in Mammalian GenomesSankar Mitra; Fiscal Year: 2007..In our previous project period we characterized human glycosylases (NEIL1 and NEIL2) with overlapping substrate preferences as OGG1 and NTH1...
- Repair of Oxidized Bases in Mammalian GenomesSankar Mitra; Fiscal Year: 2009..In our previous project period we characterized human glycosylases (NEIL1 and NEIL2) with overlapping substrate preferences as OGG1 and NTH1...
- Repair of Oxidized Bases in Mammalian GenomesSankar Mitra; Fiscal Year: 2010..In our previous project period we characterized human glycosylases (NEIL1 and NEIL2) with overlapping substrate preferences as OGG1 and NTH1...
- Regulation of Nrf2 SignalingMichael Freeman; Fiscal Year: 2009..Aim 2 will determine if key Keap1 Cys residues regulate association between the Neh2 domain of Nrf2 and Keap1 following reaction with thiol-reactive electrophiles...
- Mechanisms of Neurotoxicity of Combustion-smoke Inhalation to the BrainElla W Englander; Fiscal Year: 2010..abstract_text> ..
- HYPOXIA-INDUCED DNA DAMAGE AND REPAIR IN THE RAT BRAINElla Englander; Fiscal Year: 2009..abstract_text> ..
- HYPOXIA-INDUCED DNA DAMAGE AND REPAIR IN THE RAT BRAINElla W Englander; Fiscal Year: 2010..abstract_text> ..
- Repair of oxidative damage in mammalian genomesTapas K Hazra; Fiscal Year: 2010..b>NEIL2 has a 4- to 5-fold higher affinity and activity for 5-OHU in bubble vs duplex DNA, and it excises 8-oxoG from ..
- Repair of oxidative damage in mammalian genomesTAPAS HAZRA; Fiscal Year: 2009..b>NEIL2 has a 4- to 5-fold higher affinity and activity for 5-OHU in bubble vs duplex DNA, and it excises 8-oxoG from ..
- HYPOXIA-INDUCED DNA DAMAGE AND REPAIR IN THE BRAINElla Englander; Fiscal Year: 2004..This information will augment strategies seeking to reduce neuropathologies of brain hypoxia in humans. ..
- MAMMALIAN OXIDATIVE DNA DAMAGE REPAIRThomas Rosenquist; Fiscal Year: 2004..The effects of altering the level of ogg1 gene expression on the rate of mutagenesis and tumorigenesis also will be determined. ..
- HYPOXIA-INDUCED DNA DAMAGE AND REPAIR IN THE RAT BRAINElla Englander; Fiscal Year: 2007....
- Repair of oxidative damage in mammalian genomesTAPAS HAZRA; Fiscal Year: 2007..b>NEIL2 has a 4- to 5-fold higher affinity and activity for 5-OHU in bubble vs duplex DNA, and it excises 8-oxoG from ..
- Mechanisms of Neurotoxicity of Combustion-smoke Inhalation to the BrainElla Englander; Fiscal Year: 2007....