gag

Summary

Gene Symbol: gag
Description: Pr55(Gag)
Species: human immunodeficiency virus 1

Top Publications

  1. ncbi Vpu and Tsg101 regulate intracellular targeting of the human immunodeficiency virus type 1 core protein precursor Pr55gag
    Kirsi Harila
    Department of Virology, Haartman Institute, P O Box 21, FIN 00014 University of Helsinki, Finland
    J Virol 80:3765-72. 2006
  2. ncbi Flexible use of nuclear import pathways by HIV-1
    Kyeongeun Lee
    HIV Drug Resistance Program, National Cancer Institute, Frederick, MD 21702, USA
    Cell Host Microbe 7:221-33. 2010
  3. ncbi The cytoplasmic body component TRIM5alpha restricts HIV-1 infection in Old World monkeys
    Matthew Stremlau
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 427:848-53. 2004
  4. ncbi HIV-1 and Ebola virus encode small peptide motifs that recruit Tsg101 to sites of particle assembly to facilitate egress
    J Martin-Serrano
    Aaron Diamond AIDS Research Center and The Rockefeller University, New York, New York, USA
    Nat Med 7:1313-9. 2001
  5. ncbi Cellular microRNA and P bodies modulate host-HIV-1 interactions
    Robin Nathans
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
    Mol Cell 34:696-709. 2009
  6. ncbi Site-specific characterization of HIV-1 nucleocapsid protein binding to oligonucleotides with two binding sites
    Sergiy V Avilov
    Laboratoire Biophotonique et Pharmacologie, UMR 7213 CNRS, Universite de Strasbourg, Faculte de Pharmacie, 74 route du Rhin, 67401 Illkirch, France
    Biochemistry 48:2422-30. 2009
  7. ncbi Residues in the HIV-1 capsid assembly inhibitor binding site are essential for maintaining the assembly-competent quaternary structure of the capsid protein
    Vanda Bartonova
    Department of Virology, Universitatsklinikum Heidelberg, Im Neuenheimer Feld 324, 69120 Heidelberg, Germany
    J Biol Chem 283:32024-33. 2008
  8. ncbi Matrix mediates the functional link between human immunodeficiency virus type 1 RNA nuclear export elements and the assembly competency of Gag in murine cells
    Nathan M Sherer
    Department of Infectious Diseases, King s College School of Medicine, London SE1 9RT, United Kingdom
    J Virol 83:8525-35. 2009
  9. ncbi Tsg101 and the vacuolar protein sorting pathway are essential for HIV-1 budding
    J E Garrus
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT 84132, USA
    Cell 107:55-65. 2001
  10. ncbi Innate antiviral response targets HIV-1 release by the induction of ubiquitin-like protein ISG15
    Atsushi Okumura
    The Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins University, Baltimore, MD 21231, USA
    Proc Natl Acad Sci U S A 103:1440-5. 2006

Research Grants

  1. Palmitoylation of Hedgehog Proteins
    Marilyn D Resh; Fiscal Year: 2010
  2. TRAINING PROGRAM IN MOLECULAR AND CELLULAR BIOLOGY
    MARILYN RESH; Fiscal Year: 2007
  3. Glial cell differentiation and glioma formation
    MARILYN RESH; Fiscal Year: 2006
  4. Role of Lysyl-tRNA Synthetase in HIV-1 Replication
    Lawrence Kleiman; Fiscal Year: 2006
  5. Membrane targeting of fatty acylated proto-oncoproteins
    MARILYN RESH; Fiscal Year: 2005
  6. STRUCTURE AND FUNCTION OF HIV GAG PROTEIN
    MARILYN RESH; Fiscal Year: 2007
  7. Development of the Gag/Lysyl tRNA synthetase interaction as a target for anti-HIV
    Lawrence Kleiman; Fiscal Year: 2010
  8. Palmitoylation of Hedgehog Proteins
    Marilyn D Resh; Fiscal Year: 2010
  9. STRUCTURE AND FUNCTION OF HIV GAG PROTEIN
    MARILYN RESH; Fiscal Year: 2009
  10. MEMBRANE TARGETING OF FATTY ACYLATED PROTOONCOPROTEINS
    MARILYN RESH; Fiscal Year: 2001

Scientific Experts

Detail Information

Publications115 found, 100 shown here

  1. ncbi Vpu and Tsg101 regulate intracellular targeting of the human immunodeficiency virus type 1 core protein precursor Pr55gag
    Kirsi Harila
    Department of Virology, Haartman Institute, P O Box 21, FIN 00014 University of Helsinki, Finland
    J Virol 80:3765-72. 2006
    ..These results identified a previously unknown role for Vpu and Tsg101 as regulators for the endocytic uptake of Pr55gag and suggested that the site of HIV-1 assembly is determined by factors that regulate the endocytosis of Pr55gag...
  2. ncbi Flexible use of nuclear import pathways by HIV-1
    Kyeongeun Lee
    HIV Drug Resistance Program, National Cancer Institute, Frederick, MD 21702, USA
    Cell Host Microbe 7:221-33. 2010
    ..These findings reveal a remarkable flexibility in HIV-1 nuclear transport and highlight a single residue in CA as essential in regulating interactions with NUPs...
  3. ncbi The cytoplasmic body component TRIM5alpha restricts HIV-1 infection in Old World monkeys
    Matthew Stremlau
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 427:848-53. 2004
    ..Our studies identify TRIM5alpha as a species-specific mediator of innate cellular resistance to HIV-1 and reveal host cell components that modulate the uncoating of a retroviral capsid...
  4. ncbi HIV-1 and Ebola virus encode small peptide motifs that recruit Tsg101 to sites of particle assembly to facilitate egress
    J Martin-Serrano
    Aaron Diamond AIDS Research Center and The Rockefeller University, New York, New York, USA
    Nat Med 7:1313-9. 2001
    Retroviral Gag proteins encode sequences, termed late domains, which facilitate the final stages of particle budding from the plasma membrane...
  5. ncbi Cellular microRNA and P bodies modulate host-HIV-1 interactions
    Robin Nathans
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
    Mol Cell 34:696-709. 2009
    ..Thus we provide an example of a single host miRNA regulating HIV-1 production and infectivity. These studies highlight the significance of miRNAs and P bodies in modulating host cell interactions with HIV-1 and possibly other viruses...
  6. ncbi Site-specific characterization of HIV-1 nucleocapsid protein binding to oligonucleotides with two binding sites
    Sergiy V Avilov
    Laboratoire Biophotonique et Pharmacologie, UMR 7213 CNRS, Universite de Strasbourg, Faculte de Pharmacie, 74 route du Rhin, 67401 Illkirch, France
    Biochemistry 48:2422-30. 2009
    ..Moreover, our data suggest that binding of NC even to close binding sites shows no strong cooperativity...
  7. ncbi Residues in the HIV-1 capsid assembly inhibitor binding site are essential for maintaining the assembly-competent quaternary structure of the capsid protein
    Vanda Bartonova
    Department of Virology, Universitatsklinikum Heidelberg, Im Neuenheimer Feld 324, 69120 Heidelberg, Germany
    J Biol Chem 283:32024-33. 2008
    Morphogenesis of infectious HIV-1 involves budding of immature virions followed by proteolytic disassembly of the Gag protein shell and subsequent assembly of processed capsid proteins (CA) into the mature HIV-1 core...
  8. ncbi Matrix mediates the functional link between human immunodeficiency virus type 1 RNA nuclear export elements and the assembly competency of Gag in murine cells
    Nathan M Sherer
    Department of Infectious Diseases, King s College School of Medicine, London SE1 9RT, United Kingdom
    J Virol 83:8525-35. 2009
    ..Human immunodeficiency virus type 1 (HIV-1) assembles poorly in murine cells, reflecting inefficient targeting of the Gag structural polyprotein to the plasma membrane...
  9. ncbi Tsg101 and the vacuolar protein sorting pathway are essential for HIV-1 budding
    J E Garrus
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT 84132, USA
    Cell 107:55-65. 2001
    ..The UEV domain of Tsg101 binds to an essential tetrapeptide (PTAP) motif within the p6 domain of the structural Gag protein and also to ubiquitin...
  10. ncbi Innate antiviral response targets HIV-1 release by the induction of ubiquitin-like protein ISG15
    Atsushi Okumura
    The Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins University, Baltimore, MD 21231, USA
    Proc Natl Acad Sci U S A 103:1440-5. 2006
    ..ISG15 expression specifically inhibited ubiquitination of Gag and Tsg101 and disrupted the interaction of the Gag L domain with Tsg101, but conjugation of ISG15 to Gag or Tsg101 ..
  11. ncbi Sialoadhesin expressed on IFN-induced monocytes binds HIV-1 and enhances infectivity
    Hans Rempel
    Department of Laboratory Medicine at the Veterans Affairs Medical Center, San Francisco, California, United States of America
    PLoS ONE 3:e1967. 2008
    ....
  12. ncbi The putative alpha helix 2 of human immunodeficiency virus type 1 Vpr contains a determinant which is responsible for the nuclear translocation of proviral DNA in growth-arrested cells
    Z Nie
    Departement de Microbiologie et Immunologie, Faculte de Medecine, Universite de Montreal, Quebec, Canada
    J Virol 72:4104-15. 1998
    ..In particular, Vpr and Gag matrix (MA) were recognized to be involved in the nuclear transport of the viral preintegration complex...
  13. ncbi Analysis of human immunodeficiency virus type 1 Gag ubiquitination
    Eva Gottwein
    Abteilung Virologie, Im Neuenheimer Feld 324, 69120 Heidelberg, Germany
    J Virol 79:9134-44. 2005
    ..of human immunodeficiency virus type 1 (HIV-1) and several other retroviruses, but the functional significance of Gag ubiquitination is unknown. To address this problem, we decided to analyze Gag ubiquitination in detail...
  14. ncbi HIV-1 nucleocapsid protein switches the pathway of transactivation response element RNA/DNA annealing from loop-loop "kissing" to "zipper"
    My Nuong Vo
    Department of Chemistry, University of Minnesota, Minneapolis, MN 55455, USA
    J Mol Biol 386:789-801. 2009
    ....
  15. ncbi The nucleocapsid region of HIV-1 Gag cooperates with the PTAP and LYPXnL late domains to recruit the cellular machinery necessary for viral budding
    Vincent Dussupt
    Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America
    PLoS Pathog 5:e1000339. 2009
    HIV-1 release is mediated through two motifs in the p6 region of Gag, PTAP and LYPX(n)L, which recruit cellular proteins Tsg101 and Alix, respectively...
  16. ncbi Dynamics of HIV-1 assembly and release
    Sergey Ivanchenko
    Physical Chemistry, Department of Chemistry and Biochemistry, Munich Center for Integrated Protein Science CiPSM and Center for NanoScience, Ludwig Maximilians Universitat Munchen, Munich, Germany
    PLoS Pathog 5:e1000652. 2009
    ..release of human immunodeficiency virus (HIV) occur at the plasma membrane of infected cells and are driven by the Gag polyprotein...
  17. ncbi AIP1/ALIX is a binding partner for HIV-1 p6 and EIAV p9 functioning in virus budding
    Bettina Strack
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute and Department of Pathology, Harvard Medical School, Boston, MA 02115, USA
    Cell 114:689-99. 2003
    ..The primary late assembly (L) domain in the p6 region of HIV-1 Gag mediates the detachment of the virion by recruiting host Tsg101, a component of the class E vacuolar protein ..
  18. ncbi A single amino acid change in the SPRY domain of human Trim5alpha leads to HIV-1 restriction
    Melvyn W Yap
    Division of Virology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, United Kingdom
    Curr Biol 15:73-8. 2005
    ..A single amino acid substitution (R332P) in the human Trim5alpha can confer the ability to restrict HIV-1, suggesting that small changes during evolution may have profound effects on our susceptibility to cross-species infection...
  19. ncbi Solution structure of a double mutant of the carboxy-terminal dimerization domain of the HIV-1 capsid protein
    Hing C Wong
    Comprehensive Cancer Center, The University of Alabama at Birmingham, Birmingham, Alabama 35294 2041, USA
    Biochemistry 47:2289-97. 2008
    ..interface that includes the major homology region (MHR) has been suggested as the functional form during the Gag assembly...
  20. ncbi The Src kinase Lck facilitates assembly of HIV-1 at the plasma membrane
    Amy B Strasner
    Intergrated Bioscience Program in Immunobiology, Huck Institute of the Life Sciences, Pennsylvania State University, University Park, PA 16802, USA
    J Immunol 181:3706-13. 2008
    HIV type 1 (HIV-1) assembly and egress are driven by the viral protein Gag and occur at the plasma membrane in T cells...
  21. ncbi Entropic switch regulates myristate exposure in the HIV-1 matrix protein
    Chun Tang
    Howard Hughes Medical Institute and Department of Chemistry and Biochemistry, University of Maryland Baltimore County, 1000 Hilltop Circle, Baltimore, MD 21250 5398, USA
    Proc Natl Acad Sci U S A 101:517-22. 2004
    ..myristoylated matrix protein (myr-MA) of HIV functions as a regulator of intracellular localization, targeting the Gag precursor polyprotein to lipid rafts in the plasma membrane during virus assembly and dissociating from the ..
  22. ncbi Distinct intracellular trafficking of equine infectious anemia virus and human immunodeficiency virus type 1 Gag during viral assembly and budding revealed by bimolecular fluorescence complementation assays
    Jing Jin
    Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, W1144 Biomedical Science Tower, Pittsburgh, PA 15261, USA
    J Virol 81:11226-35. 2007
    Retroviral Gag polyproteins are necessary and sufficient for virus budding...
  23. ncbi Dynamics and timing of in vivo mutations at Gag residue 242 during primary HIV-1 subtype C infection
    Vladimir Novitsky
    Harvard School of Public Health AIDS Initiative, Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, MA 02115, USA
    Virology 403:37-46. 2010
    Viral mutations at Gag residue 242 and relevant viral polymorphisms were analyzed in a cohort of 42 individuals with primary HIV-1 subtype C infection using single-genome amplification/sequencing...
  24. ncbi Gag mutations strongly contribute to HIV-1 resistance to protease inhibitors in highly drug-experienced patients besides compensating for fitness loss
    Elisabeth Dam
    INSERM U552, Paris, France
    PLoS Pathog 5:e1000345. 2009
    ..Additional mutations compensating for the RC loss subsequently accumulate within PR and in Gag substrate cleavage sites...
  25. ncbi Gyrase B inhibitor impairs HIV-1 replication by targeting Hsp90 and the capsid protein
    Luciano Vozzolo
    Wohl Virion Centre, Division of Infection and Immunity, University College London, 46 Cleveland Street, W1T 4JF London, United Kingdom
    J Biol Chem 285:39314-28. 2010
    ..Both activities were amenable to inhibition by small molecules and represent novel antiretroviral drug targets...
  26. ncbi Host HLA B*allele-associated multi-clade Gag T-cell recognition correlates with slow HIV-1 disease progression in antiretroviral therapy-naïve Ugandans
    Jennifer Serwanga
    MRC UVRI Uganda Research Unit on AIDS, c o Uganda Virus Research Institute, Entebbe, Uganda
    PLoS ONE 4:e4188. 2009
    ..In this study, possible interactions between viral load, CD4 T cell slopes, host genetics and HIV-specific IFN-gamma responses were evaluated in chronically HIV-1-infected adults...
  27. ncbi Differential membrane binding of the human immunodeficiency virus type 1 matrix protein
    W Zhou
    Cell Biology and Genetics Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    J Virol 70:8540-8. 1996
    ..binding signal within the amino-terminal 31 amino acids of the matrix domain of human immunodeficiency virus type 1 Gag, consisting of myristate and a highly basic region (W. Zhou, L. J. Parent, J. W. Wills, and M. D. Resh, J. Virol...
  28. ncbi Cyclophilin A is required for the replication of group M human immunodeficiency virus type 1 (HIV-1) and simian immunodeficiency virus SIV(CPZ)GAB but not group O HIV-1 or other primate immunodeficiency viruses
    D Braaten
    Department of Microbiology, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA
    J Virol 70:4220-7. 1996
    The human immunodeficiency virus type 1 (HIV-1) Gag polyprotein binds to cyclophilin A and incorporates this cellular peptidyl prolyl-isomerase into virions...
  29. ncbi Covalent modification of human immunodeficiency virus type 1 p6 by SUMO-1
    Cagan Gurer
    Department of Microbiology, Columbia University, 701 W 168th St, New York, NY 10032, USA
    J Virol 79:910-7. 2005
    The p6 domain of the human immunodeficiency virus type 1 (HIV-1) Gag polyprotein mediates virion budding from infected cells via protein-protein contacts with the class E vacuolar protein sorting factors, Tsg101 and AIP1/ALIX...
  30. ncbi Effect of Mg(2+) and Na(+) on the nucleic acid chaperone activity of HIV-1 nucleocapsid protein: implications for reverse transcription
    My Nuong Vo
    Department of Chemistry and Institute for Molecular Virology, University of Minnesota, Minneapolis, MN 55455, USA
    J Mol Biol 386:773-88. 2009
    ....
  31. ncbi Human immunodeficiency virus type 1 reverse transcriptase: enhancement of activity by interaction with cellular topoisomerase I
    H Takahashi
    Department of Pathology, National Institute of Health, Tokyo, Japan
    Proc Natl Acad Sci U S A 92:5694-8. 1995
    ..We have investigated a possible association of HIV-1 gag proteins with topo I activity...
  32. ncbi Role of the karyopherin pathway in human immunodeficiency virus type 1 nuclear import
    P Gallay
    Infectious Disease Laboratory, Salk Institute for Biological Studies, La Jolla, California 92037, USA
    J Virol 70:1027-32. 1996
    ..In addition, our data suggest that Vpr governs HIV-1 nuclear import through a distinct pathway...
  33. ncbi Human TRIM5alpha mediated restriction of different HIV-1 subtypes and Lv2 sensitive and insensitive HIV-2 variants
    Patrick Kaumanns
    Department of Virology, University of Heidelberg, Im Neuenheimer Feld 324, D 69120 Heidelberg, Germany
    Retrovirology 3:79. 2006
    ..2 and 19.6 fold restriction)...
  34. ncbi Electrostatic interactions drive membrane association of the human immunodeficiency virus type 1 Gag MA domain
    Amanda K Dalton
    Department of Molecular Biology and Genetics, Biotechnology Building, Cornell University, Ithaca, NY 14853, USA
    J Virol 81:6434-45. 2007
    ..Membrane association is directed by MA, the N-terminal domain of the Gag structural protein...
  35. ncbi Nucleolin and the packaging signal, psi, promote the budding of human immunodeficiency virus type-1 (HIV-1)
    Tomonori Ueno
    Department of Pathology, National Institute of Infectious Diseases, Tokyo, Japan
    Microbiol Immunol 48:111-8. 2004
    b>Gag proteins of human immunodeficiency virus type 1 (HIV-1) play a pivotal role in the budding of the virion, in which the zinc finger motifs of the gag proteins recognize the packaging signal of genomic RNA...
  36. ncbi HIV-1 matrix protein p17 enhances the proliferative activity of natural killer cells and increases their ability to secrete proinflammatory cytokines
    Marco Vitale
    Department of Anatomy, Pharmacology and Forensic Medicine, Ospedale Maggiore, University of Parma, Italy
    Br J Haematol 120:337-43. 2003
    ..The property of p17 to increase the production of TNF-alpha and IFN-gamma might be a mechanism used by HIV-1 to modulate the immune system to support its replication and spreading...
  37. ncbi The tRNALys packaging complex in HIV-1
    Lawrence Kleiman
    Lady Davis Institute for Medical Research, Jewish General Hospital, 3755 Cote St Catherine Road, Montreal, Que, Canada H3T 1E2
    Int J Biochem Cell Biol 36:1776-86. 2004
    ..supports a model for the formation of a tRNALys packaging complex, whose components include the precursor proteins Gag and Gag-Pol, viral genomic RNA, tRNALys, and lysyl-tRNA synthetase (LysRS)...
  38. ncbi Influence of dominant HIV-1 epitopes on HLA-A3/peptide complex formation
    Judith Racape
    Departement d Immunologie, Institut Cochin, F 75014 Paris, France
    Proc Natl Acad Sci U S A 103:18208-13. 2006
    ..Such observations may have great implications for T cell antigen receptor recognition and the immunogenicity of HLA/peptide complexes...
  39. ncbi Refined study of the interaction between HIV-1 p6 late domain and ALIX
    Carine Lazert
    Universite Lyon 1, Centre National de Recherche Scientifique CNRS, VirPatH FRE 3011, Faculté de médecine RTH Laennec, Lyon, France
    Retrovirology 5:39. 2008
    ..Altogether, our data support a model where the C-terminal proline-rich domain of ALIX allows the access of its binding site to p6 by alleviating a conformational constraint resulting from the presence of the central p6 hinge...
  40. ncbi Novel activities of cyclophilin A and cyclosporin A during HIV-1 infection of primary lymphocytes and macrophages
    Manisha Saini
    Molecular Virology Division, St Luke s Roosevelt Hospital Center, Columbia University Medical Center, 432 West 58th Street, New York, NY 10019, USA
    J Immunol 177:443-9. 2006
    ..at both early and late phases of virus replication, the stage affected is determined by the sequence of HIV-1 Gag. Because the cell type infected determines the identity of host proteins active in HIV-1 replication and can ..
  41. ncbi Structural studies of HIV-1 Gag p6ct and its interaction with Vpr determined by solution nuclear magnetic resonance
    Gilmar F Salgado
    Unite de Pharmacologie Chimique et Genetique, INSERM U640, CNRS UMR8151, UFR des Sciences Pharmaceutiques et Biologiques, 4, avenue de l Observatoire, 75270 Paris Cedex 06, France
    Biochemistry 48:2355-67. 2009
    ..protein R (Vpr) incorporation in sorting virions relies greatly on the interaction with the group-specific antigen (Gag) C-terminal region, which encompasses protein p6...
  42. ncbi HIV p17 enhances lymphocyte proliferation and HIV-1 replication after binding to a human serum factor
    M A De Francesco
    Institute of Microbiology, Brescia University Medical School, Italy
    AIDS 12:245-52. 1998
    ..To analyse the role of recombinant HIV-1 protein p17 in the modulation of cell activity...
  43. ncbi HIV-1 nuclear import: in search of a leader
    M I Bukrinsky
    The Picower Institute for Medical Research, Manhasset, NY 11030, USA
    Front Biosci 2:d578-87. 1997
    ..We also describe a class of novel anti-HIV compounds which target the NLSs of HIV-1 and effectively block viral replication in T cells and macrophages...
  44. ncbi Structural basis of the association of HIV-1 matrix protein with DNA
    Mengli Cai
    Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, United States of America
    PLoS ONE 5:e15675. 2010
    ..We show that MA interacts with DNA and this is likely sufficient to account for its association with the PIC...
  45. ncbi The Nef protein of human immunodeficiency virus type 1 enhances serine phosphorylation of the viral matrix
    S Swingler
    Infectious Disease Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037, USA
    J Virol 71:4372-7. 1997
    ..Recombinant p21-activated kinase hPAK65, a recently proposed relative of the Nef-associated kinase, achieved a comparable result. Taken together, these data suggest that MA is a target of the Nef-associated serine kinase...
  46. ncbi Gag induces the coalescence of clustered lipid rafts and tetraspanin-enriched microdomains at HIV-1 assembly sites on the plasma membrane
    Ian B Hogue
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    J Virol 85:9749-66. 2011
    The HIV-1 structural protein Gag associates with two types of plasma membrane microdomains, lipid rafts and tetraspanin-enriched microdomains (TEMs), both of which have been proposed to be platforms for HIV-1 assembly...
  47. ncbi Human plasmatic apolipoprotein H binds human immunodeficiency virus type 1 and type 2 proteins
    E Stefas
    OSRTOM, UR41, Montpellier, France
    AIDS Res Hum Retroviruses 13:97-104. 1997
    ..assay and ligand blot and dot assays, apo H was able to bind recombinant retroviral HIV antigens, especially Gag proteins p18 of HIV-1, p26 of HIV-2, and Env gp160 of HIV-1...
  48. ncbi Structural similarity between the p17 matrix protein of HIV-1 and interferon-gamma
    S Matthews
    Department of Biochemistry, University of Oxford, UK
    Nature 370:666-8. 1994
    ..The protein has several key functions. It orchestrates viral assembly via targeting signals that direct the gag precursor polyprotein, p55, to the host cell membrane and it interacts with the transmembrane protein, gp41, to ..
  49. ncbi High prevalence of natural polymorphisms in Gag (CA-SP1) associated with reduced response to Bevirimat, an HIV-1 maturation inhibitor
    Eduardo Seclen
    Department of Infectious Diseases, Hospital Carlos III, Madrid, Spain
    AIDS 24:467-9. 2010
    ..Conversely, only four patients (1%) harbored major bevirimat resistance mutations. Finally, specific gag changes were associated with protease inhibitor resistance mutations in subtype B viruses.
  50. ncbi Highly sensitive analysis of the interaction between HIV-1 Gag and phosphoinositide derivatives based on surface plasmon resonance
    Kensaku Anraku
    Institute of Health Sciences, Kumamoto Health Science University, 325 Izumi machi, Kumamoto 861 5598, Japan
    Biochemistry 49:5109-16. 2010
    Human immunodeficiency virus type 1 (HIV-1) Gag protein is the principal structural component of the HIV particle. Localization of the Pr55(Gag) protein to the plasma membrane initiates virus assembly...
  51. ncbi Analysis of the percentage of human immunodeficiency virus type 1 sequences that are hypermutated and markers of disease progression in a longitudinal cohort, including one individual with a partially defective Vif
    Anne Piantadosi
    Fred Hutchinson Cancer Center, Seattle, WA 98109 1024, USA
    J Virol 83:7805-14. 2009
    ..By examining single-copy gag sequences from proviral DNA, hypermutation was detected in 16 of 28 individuals...
  52. ncbi CNI-H0294, a nuclear importation inhibitor of the human immunodeficiency virus type 1 genome, abrogates virus replication in infected activated peripheral blood mononuclear cells
    O K Haffar
    Bristol Myers Squibb Pharmaceutical Research Institute, Seattle, Washington 98121, USA
    Antimicrob Agents Chemother 42:1133-8. 1998
    ..These results support nuclear importation as a novel target and CNI-H0294 and its derivatives as novel compounds for therapeutic intervention in HIV infection and AIDS...
  53. ncbi HIV-1 nuclear import: matrix protein is back on center stage, this time together with Vpr
    M I Bukrinsky
    Picower Institute for Medical Research, Manhasset, NY 11030, USA
    Mol Med 4:138-43. 1998
  54. ncbi Enhancement of the basal-level activity of HIV-1 long terminal repeat by HIV-1 nucleocapsid protein
    J L Zhang
    Division of Infectious Diseases, Charles A Dana Research Institute, Boston, Massachusetts, USA
    Virology 268:251-63. 2000
    ..Transient transfection of human T lymphocytes with the plasmids containing HIV-1 nc or gag showed enhancement of LTR-CAT activity...
  55. ncbi The nucleocapsid region of human immunodeficiency virus type 1 Gag assists in the coordination of assembly and Gag processing: role for RNA-Gag binding in the early stages of assembly
    David E Ott
    AIDS and Cancer Virus Program, SAIC Frederick, Inc, National Cancer Institute at Frederick, Frederick, MD 21702 1201, USA
    J Virol 83:7718-27. 2009
    Human immunodeficiency virus type 1 (HIV-1) Gag-RNA interactions are required for virus assembly...
  56. ncbi Species-specific variation in the B30.2(SPRY) domain of TRIM5alpha determines the potency of human immunodeficiency virus restriction
    Matthew Stremlau
    Dana Farber Cancer Institute, 44 Binney St, JFB 824, Boston, MA 02115, USA
    J Virol 79:3139-45. 2005
    ..These observations implicate the v1 variable region of the B30.2(SPRY) domain in TRIM5alpha(rh) antiviral potency...
  57. ncbi Human immunodeficiency virus type 1 preintegration complexes: studies of organization and composition
    M D Miller
    Infectious Disease Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037, USA
    J Virol 71:5382-90. 1997
    ..Only molecules correctly cleaved by integrase protein at the 3' ends were competent to integrate, suggesting that one role for terminal cleavage by integrase may be to create a defined end at otherwise heterogeneous cDNA termini...
  58. ncbi HIV-1 Gag processing intermediates trans-dominantly interfere with HIV-1 infectivity
    Barbara Muller
    Department of Virology, Universitatsklinikum Heidelberg, Im Neuenheimer Feld 324, D 69120 Heidelberg, Germany
    J Biol Chem 284:29692-703. 2009
    ..HIV) polyprotein processing, but there is no direct quantitative correlation between the degree of impairment of Gag processing and virion infectivity at low PI concentrations...
  59. ncbi Myristoylation is required for human immunodeficiency virus type 1 Gag-Gag multimerization in mammalian cells
    Hua Li
    Department of Pediatrics, Emory University, Atlanta, GA 30322, USA
    J Virol 81:12899-910. 2007
    The Gag protein of human immunodeficiency virus type 1 directs the virion assembly process. Gag proteins must extensively multimerize during the formation of the spherical immature virion shell...
  60. ncbi Translation elongation factor 1-alpha interacts specifically with the human immunodeficiency virus type 1 Gag polyprotein
    A Cimarelli
    Departments of Microbiology, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA
    J Virol 73:5388-401. 1999
    Human immunodeficiency virus type 1 (HIV-1) gag-encoded proteins play key functions at almost all stages of the viral life cycle...
  61. ncbi Intracellular interactions between APOBEC3G, RNA, and HIV-1 Gag: APOBEC3G multimerization is dependent on its association with RNA
    Yeshitila N Friew
    HIV Drug Resistance Program, National Cancer Institute Frederick, Frederick, Maryland 21702 1201, USA
    Retrovirology 6:56. 2009
    ..is a dimer and its virion incorporation is mediated through interactions with viral or nonviral RNAs and/or HIV-1 Gag. We have now employed a bimolecular fluorescence complementation assay (BiFC) to analyze the intracellular A3G-A3G, ..
  62. ncbi Viral protein R regulates nuclear import of the HIV-1 pre-integration complex
    S Popov
    The Picower Institute for Medical Research, Manhasset, NY 11030, USA
    EMBO J 17:909-17. 1998
    ..These results identify the biochemical mechanism of Vpr function in transport of the viral pre-integration complex to, and across, the nuclear membrane...
  63. ncbi Mutations in human immunodeficiency virus type 1 nucleocapsid protein zinc fingers cause premature reverse transcription
    James A Thomas
    AIDS and Cancer Virus Program, SAIC Frederick, Inc, NCI Frederick, P O Box B, Frederick, MD 21702 1201, USA
    J Virol 82:9318-28. 2008
    ..virions bud from producer cells, but we fail to see any evidence that the NC mutations alter the timing of Pr55(Gag) processing...
  64. ncbi Unique features of TRIM5alpha among closely related human TRIM family members
    Xing Li
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Division of AIDS, Harvard Medical School, Boston, MA 02115, USA
    Virology 360:419-33. 2007
    ..In contrast to the other TRIM genes examined, TRIM5 exhibited evidence of positive selection. The unique features of TRIM5alpha among its TRIM relatives underscore its special status as an antiviral factor...
  65. ncbi Stoichiometry of the antiviral protein APOBEC3G in HIV-1 virions
    Hongzhan Xu
    Viral Mutation Section, HIV Drug Resistance Program, Center for Cancer Research, National Cancer Institute Frederick, P O Box B, Bldg 535, Rm 334, Frederick, MD 21702, USA
    Virology 360:247-56. 2007
    ..virions was proportional to the level of its expression in the viral producing cells, and the ratio of the A3G to Gag in the Deltavif virions produced from activated human peripheral blood mononuclear cells (PBMC) was approximately 1:..
  66. ncbi An Alix fragment potently inhibits HIV-1 budding: characterization of binding to retroviral YPXL late domains
    Utpal M Munshi
    Virus Cell Interaction Section, HIV Drug Resistance Program, National Cancer Institute Frederick, Frederick, Maryland 21702 1201, USA
    J Biol Chem 282:3847-55. 2007
    The retroviral structural protein, Gag, contains small peptide motifs known as late domains that promote efficient virus release from the infected cell...
  67. ncbi A mutation in alpha helix 3 of CA renders human immunodeficiency virus type 1 cyclosporin A resistant and dependent: rescue by a second-site substitution in a distal region of CA
    Ruifeng Yang
    Department of Microbiology and Immunology, Vanderbilt University School of Medicine, A 5301 Medical Center North, Nashville, TN 37232 2363, USA
    J Virol 81:3749-56. 2007
    ..A105T rescued the impaired single-cycle infectivity and replication defects of both T54A and A92E mutants. These results indicate that CA determinants outside the CypA-binding loop can modulate the dependence of HIV-1 infection on CypA...
  68. ncbi Anx2 interacts with HIV-1 Gag at phosphatidylinositol (4,5) bisphosphate-containing lipid rafts and increases viral production in 293T cells
    Alexia V Harrist
    Department of Neurology and Microbiology, University of Pennsylvania, Philadelphia, PA, USA
    PLoS ONE 4:e5020. 2009
    ..We previously defined Annexin 2 (Anx2) as an HIV Gag binding partner in human monocyte-derived macrophages (MDMs) that promotes proper viral assembly...
  69. ncbi Roles of Gag and NCp7 in facilitating tRNA(Lys)(3) Annealing to viral RNA in human immunodeficiency virus type 1
    Fei Guo
    Lady Davis Institute for Medical Research and McGill AIDS Centre, Jewish General Hospital, Montreal, Quebec, Canada
    J Virol 83:8099-107. 2009
    In protease-negative human immunodeficiency virus type 1 (HIV-1) [Pr(-)], the amount of tRNA(3)(Lys) annealed by Gag is modestly reduced ( approximately 25%) compared to that annealed by mature nucleocapsid (NCp7) in protease-positive ..
  70. ncbi Escape and compensation from early HLA-B57-mediated cytotoxic T-lymphocyte pressure on human immunodeficiency virus type 1 Gag alter capsid interactions with cyclophilin A
    Mark A Brockman
    Partners AIDS Research Center, Massachusetts General Hospital East, CNY 6625, Charlestown, MA 02129, USA
    J Virol 81:12608-18. 2007
    ..An early CD8(+) T-cell escape mutation in the dominant HLA-B57-restricted Gag epitope TW10 (TSTLQEQIGW) has been shown to impair HIV-1 replication capacity in vitro...
  71. ncbi Phosphorylation of residue 131 of HIV-1 matrix is not required for macrophage infection
    E O Freed
    Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892-0460, USA
    Cell 88:171-3; discussion 173-4. 1997
  72. ncbi Solution structure of a hydrocarbon stapled peptide inhibitor in complex with monomeric C-terminal domain of HIV-1 capsid
    Shibani Bhattacharya
    New York Structural Biology Center, New York, New York 10027, USA
    J Biol Chem 283:16274-8. 2008
    ..O., and Debnath, A. K. (2008) J. Mol. Biol. 378, 565-580). In cell-based assays, NYAD-1 colocalized with the Gag polyprotein during traffic to the plasma membrane and disrupted the formation of mature and immature virus ..
  73. ncbi Deficiency of niemann-pick type C-1 protein impairs release of human immunodeficiency virus type 1 and results in Gag accumulation in late endosomal/lysosomal compartments
    Yuyang Tang
    Center for AIDS Health Disparities Research, Meharry Medical College, Nashville, TN 37208, USA
    J Virol 83:7982-95. 2009
    ..Examination of these cells using confocal microscopy showed an accumulation and stabilization of Gag in LE/L compartments...
  74. ncbi The cell biology of HIV-1 and other retroviruses
    Eric O Freed
    Virus Cell Interaction Section, HIV Drug Resistance Program, National Cancer Institute, Frederick, MD 21702 1201, USA
    Retrovirology 3:77. 2006
    ..restrictions to retroviral infection; nuclear import and integration; gene expression/regulation of retroviral Gag and genomic RNA; and assembly/release...
  75. ncbi Myristoylation of gag proteins of HIV-1 plays an important role in virus assembly
    R Pal
    Department of Cell Biology, Advanced BioScience Laboratories, Inc, Kensington, Maryland 20895
    AIDS Res Hum Retroviruses 6:721-30. 1990
    The gag proteins of HIV-1 are modified by the addition of myristic acid to the amino terminal glycine residue. Site-directed mutagenesis was used to construct a mutant of HIV-1 in which this glycine residue was changed to an alanine...
  76. ncbi Ubiquitin is covalently attached to the p6Gag proteins of human immunodeficiency virus type 1 and simian immunodeficiency virus and to the p12Gag protein of Moloney murine leukemia virus
    D E Ott
    AIDS Vaccine Program, SAIC Frederick, National Cancer Institute, Frederick Cancer Research and Development Center, Maryland 21702 1201, USA
    J Virol 72:2962-8. 1998
    ..presented here show that all three virions incorporate ubiquitin molecules at approximately 10% of the level of Gag found in virions...
  77. ncbi Cryoelectron microscopic examination of human immunodeficiency virus type 1 virions with mutations in the cyclophilin A binding loop
    L B Kong
    Department of Molecular and Medical Pharmacology and Crump Institute for Biological Imaging, UCLA School of Medicine, Los Angeles, California 90095, USA
    J Virol 72:4403-7. 1998
    ..Although viral RNA incorporation and protease cleavage of the Gag precursor were not affected by these mutations, cryoelectron microscopy revealed a loss of virion maturation in ..
  78. ncbi N-Terminal extension of human immunodeficiency virus capsid protein converts the in vitro assembly phenotype from tubular to spherical particles
    I Gross
    Heinrich Pette Institut, D 20251 Hamburg, Germany
    J Virol 72:4798-810. 1998
    Expression of retroviral Gag polyproteins is sufficient for morphogenesis of virus-like particles with a spherical immature protein shell...
  79. ncbi Functional interaction of human immunodeficiency virus type 1 Vpu and Gag with a novel member of the tetratricopeptide repeat protein family
    M A Callahan
    McArdle Laboratory for Cancer Research, University of Wisconsin Medical School, Madison, Wisconsin 53706, USA
    J Virol 72:8461. 1998
  80. ncbi Actin associates with the nucleocapsid domain of the human immunodeficiency virus Gag polyprotein
    T Wilk
    Structural Biology Programme, European Molecular Biology Laboratory, 69117 Heidelberg, Germany
    J Virol 73:1931-40. 1999
    ..Our results show that the retroviral Gag polyprotein is sufficient for actin uptake...
  81. ncbi Possible role of the plasminogen receptor as a site of interaction of the human immunodeficiency virus p24 immunosuppressive heptapeptide Ch7 with the host immune system
    E Giacomini
    Department of Immunology, Istituto Superiore di Sanita, Rome, Italy
    Scand J Immunol 51:164-7. 2000
    ..We suggest that the plasminogen receptor may represent a target structure on human monocytes for the immunosuppressive p24 sequence...
  82. ncbi Calmodulin and HIV type 1: interactions with Gag and Gag products
    W Radding
    Department of Biochemistry and Cell Biology, Rice University, Houston, Texas 77005, USA
    AIDS Res Hum Retroviruses 16:1519-25. 2000
    ..We have, therefore, investigated whether calmodulin can interact with HIV structural proteins Gag, p17, and p24...
  83. ncbi Proteasome inhibition interferes with gag polyprotein processing, release, and maturation of HIV-1 and HIV-2
    U Schubert
    Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892 0440, USA
    Proc Natl Acad Sci U S A 97:13057-62. 2000
    ..and transport of viral proteins to the virus assembly site followed by subsequent proteolytic cleavage of the Gag polyprotein within the nascent virion...
  84. ncbi Transfer of the HIV-1 cyclophilin-binding site to simian immunodeficiency virus from Macaca mulatta can confer both cyclosporin sensitivity and cyclosporin dependence
    A A Bukovsky
    Division of Human Retrovirology, Dana Farber Cancer Institute, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 94:10943-8. 1997
    ..is mediated by a specific interaction between the active site of the enzyme and the capsid (CA) domain of the HIV-1 Gag polyprotein...
  85. ncbi HIV-1 infection of nondividing cells through the recognition of integrase by the importin/karyopherin pathway
    P Gallay
    The Salk Institute, 10010 North Torrey Pines Road, La Jolla, CA 92037 1099, USA
    Proc Natl Acad Sci U S A 94:9825-30. 1997
    ..This novel function of integrase reflects the recognition of an atypical bipartite nuclear localization signal by the importin/karyopherin pathway...
  86. ncbi Myristoylation-dependent replication and assembly of human immunodeficiency virus 1
    M Bryant
    Department of Pediatrics, Washington University School of Medicine, Saint Louis, MO 63110
    Proc Natl Acad Sci U S A 87:523-7. 1990
    ..Complementation of this defect, in trans, restores infectious particle production. The nonmyristoylated (myr-) gag precursor accumulates in infected cells and is not processed into the mature capsid components of the intact virion...
  87. ncbi Interferon-gamma is associated with the surface of the human immunodeficiency virus and binds to the gag gene product p17
    A Caruso
    Institute of Microbiology, University of Brescia, Spedali Civili, Italy
    AIDS Res Hum Retroviruses 5:605-12. 1989
    ..The specificity of the reaction was confirmed by Western blot analysis. This finding opens new questions about the biologic role of IFN-gamma itself and of its interaction with HIV...
  88. ncbi Antimyristoylation of the gag proteins in the human immunodeficiency virus-infected cells with N-myristoyl glycinal diethylacetal resulted in inhibition of virus production
    A Tashiro
    Department of Biochemistry, Faculty of Pharmaceutical Science, Kumamoto University, Japan
    Biochem Biophys Res Commun 165:1145-54. 1989
    The gag proteins of human retroviruses such as human immunodeficiency virus (HIV-1) are specifically myristoylated in their amino termini (1, 2, 3)...
  89. ncbi Cyclophilin binding to the human immunodeficiency virus type 1 Gag polyprotein is mimicked by an anti-cyclosporine antibody
    E K Franke
    Department of Medicine, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA
    J Virol 69:5821-3. 1995
    ..requires specific incorporation into virions of the cellular protein cyclophilin A through interactions with the Gag polyprotein. Here we show that monoclonal antibody B11 1...
  90. ncbi Human immunodeficiency virus proteins induce the inhibitory cAMP/protein kinase A pathway in normal lymphocytes
    B Hofmann
    Center for Interdisciplinary Research in Immunology and Disease, Johnson Comprehensive Cancer Center, University of California, Los Angeles 90024 1747
    Proc Natl Acad Sci U S A 90:6676-80. 1993
    ..These studies show that the HIV-induced augmentation of cAMP/PKA activity may be a key part of the mechanism responsible for all or part of the HIV-induced anergy of T lymphocytes...
  91. ncbi Functional association of cyclophilin A with HIV-1 virions
    M Thali
    Division of Human Retrovirology, Dana Farber Cancer Institute, Boston, Massachusetts
    Nature 372:363-5. 1994
    ..Human cyclophilins A and B are host cell proteins that bind specifically to the HIV-1 Gag polyprotein p55gag in vitro...
  92. ncbi Evidence for direct association of Vpr and matrix protein p17 within the HIV-1 virion
    A Sato
    Shionogi Institute for Medical Science, Osaka, Japan
    Virology 220:208-12. 1996
    ..of HIV-1 and is selectively incorporated into the virion by a process involving the C-terminal p6 portion of the Gag precursor Pr55...
  93. ncbi Membrane binding of human immunodeficiency virus type 1 matrix protein in vivo supports a conformational myristyl switch mechanism
    P Spearman
    Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 2581, USA
    J Virol 71:6582-92. 1997
    The interaction of the human immunodeficiency virus (HIV) Gag protein with the plasma membrane of a cell is a critical event in the assembly of HIV particles...
  94. ncbi A role for ubiquitin ligase recruitment in retrovirus release
    B Strack
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 97:13063-8. 2000
    Retroviral Gag polyproteins have specific regions, commonly referred to as late assembly (L) domains, which are required for the efficient separation of assembled virions from the host cell...
  95. ncbi Amino-terminal fragment of urokinase-type plasminogen activator inhibits HIV-1 replication
    M Wada
    Laboratories for Bioengineering and Research, JCR Pharmaceuticals Company, Ltd, 2 2 10 Murotani, Nishi ku, Kobe, 651 2241, Japan
    Biochem Biophys Res Commun 284:346-51. 2001
    ..These findings suggest that ATF may inhibit the assembly and budding of HIV-1, which provides a novel therapeutic strategy for AIDS...
  96. ncbi Tal, a Tsg101-specific E3 ubiquitin ligase, regulates receptor endocytosis and retrovirus budding
    Ido Amit
    Department of Biological Regulation, The Weizmann Institute of Science, Rehovot 76100, Israel
    Genes Dev 18:1737-52. 2004
    ....
  97. ncbi Matrix-induced inhibition of membrane binding contributes to human immunodeficiency virus type 1 particle assembly defects in murine cells
    Theodora Hatziioannou
    Aaron Diamond AIDS Research Center, 455 First Avenue, New York, NY 10016, USA
    J Virol 79:15586-9. 2005
    ..assembly in murine cells is accompanied by poor plasma membrane binding and proteolytic processing of the HIV-1 Gag precursor...
  98. ncbi Molecular characterization of the HIV-1 gag nucleocapsid gene associated with vertical transmission
    Brian P Wellensiek
    Department of Microbiology and Immunology, College of Medicine, The University of Arizona Health Sciences Center, Tucson, Arizona, USA
    Retrovirology 3:21. 2006
    ..Another gag gene product, p6, plays an integral role in successful viral budding from the plasma membrane and inclusion of the ..
  99. ncbi Assembly of infectious HIV-1 in human epithelial and T-lymphoblastic cell lines
    Boyan Grigorov
    LaboRetro Unité de virologie humaine INSERM U758, IFR128 Biosciences Lyon Gerland, Ecole Normale Superieure de Lyon, 46 allee d Italie, 69 364 Lyon, France
    J Mol Biol 359:848-62. 2006
    ..this question, we investigated the intracellular location of the major viral structural components of HIV-1, namely Gag, Env and the genomic RNA...
  100. ncbi Mapping of tetraspanin-enriched microdomains that can function as gateways for HIV-1
    Sascha Nydegger
    Graduate Program in Microbiology and Molecular Genetics, University of Vermont, Burlington, VT 05405, USA
    J Cell Biol 173:795-807. 2006
    ..Further, we reveal that the human immunodeficiency virus type 1 (HIV-1) Gag protein, which directs viral assembly and release, accumulates at surface TEMs together with the HIV-1 envelope ..
  101. ncbi Functional replacement of the RING, B-box 2, and coiled-coil domains of tripartite motif 5alpha (TRIM5alpha) by heterologous TRIM domains
    Xing Li
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, 44 Binney Street JFB 824, Boston, MA 02115, USA
    J Virol 80:6198-206. 2006
    ..These results indicate that heterologous RING, B-box 2, and CC domains from related TRIM proteins can functionally substitute for TRIM5alpha(rh) domains...

Research Grants62

  1. Palmitoylation of Hedgehog Proteins
    Marilyn D Resh; Fiscal Year: 2010
    ..abstract_text> ..
  2. TRAINING PROGRAM IN MOLECULAR AND CELLULAR BIOLOGY
    MARILYN RESH; Fiscal Year: 2007
    ..We are therefore requesting continuation of this grant, with a level of funding to support a total of 8 first and second year graduate students. ..
  3. Glial cell differentiation and glioma formation
    MARILYN RESH; Fiscal Year: 2006
    ..The activity of the above proteins in human glioma tumor samples will be determined. ..
  4. Role of Lysyl-tRNA Synthetase in HIV-1 Replication
    Lawrence Kleiman; Fiscal Year: 2006
    ..b>Gag alone will package LysRS into Gag particles...
  5. Membrane targeting of fatty acylated proto-oncoproteins
    MARILYN RESH; Fiscal Year: 2005
    ..We will test whether SFKs interact with components of the endocytic machinery en route to the plasma membrane. ..
  6. STRUCTURE AND FUNCTION OF HIV GAG PROTEIN
    MARILYN RESH; Fiscal Year: 2007
    The overall goal of the research proposed in this renewal application is to understand how HIV-1 Gag is trafficked through the cell, targeted to membranes and assembled into virions...
  7. Development of the Gag/Lysyl tRNA synthetase interaction as a target for anti-HIV
    Lawrence Kleiman; Fiscal Year: 2010
    ..The selective packaging of tRNALys3 occurs because the viral protein Gag specifically interacts with a major tRNALys3-binding protein in the cell, lysyl-tRNA synthetase (LysRS), so that ..
  8. Palmitoylation of Hedgehog Proteins
    Marilyn D Resh; Fiscal Year: 2010
    ..The proposed studies will help us understand how Hh proteins work in normal and malignant cells and will aim to develop Hh inhibitors that could potentially be clinically useful as anti-tumor agents in Hh driven malignancies. ..
  9. STRUCTURE AND FUNCTION OF HIV GAG PROTEIN
    MARILYN RESH; Fiscal Year: 2009
    The overall goal of the research proposed in this renewal application is to understand how HIV-1 Gag is trafficked through the cell, targeted to membranes and assembled into virions...
  10. MEMBRANE TARGETING OF FATTY ACYLATED PROTOONCOPROTEINS
    MARILYN RESH; Fiscal Year: 2001
    ..We will test the abilities of wt, dominant negative and fatty acylation mutants of Fyn to promote the oligodendrocyte differentiation process. ..
  11. STRUCTURE AND FUNCTION OF HIV GAG PROTEIN
    MARILYN RESH; Fiscal Year: 2003
    ..This will be accomplished by studying the HIV-1 Gag polyprotein, Pr55gag. Use of HIV-1 as a model system is dually advantageous...
  12. Regulation of APOBEC3G enzymatic activity in HIV-infected primary human T cells
    JAISRI LINGAPPA; Fiscal Year: 2009
    ....
  13. Regulation of APOBEC3G enzymatic activity in HIV-infected primary human T cells
    Jaisri R Lingappa; Fiscal Year: 2010
    ....
  14. Design and Testing of a Parechovirus Vaccine Vector
    Paul Krogstad; Fiscal Year: 2005
    ..We propose to construct and complete the initial in vitro testing of a parechovirus based vaccine vector, based on strategies already employed in the successful construction of enteroviral vectors. ..
  15. Cellular Immunology of Enterovirus Infection
    Paul Krogstad; Fiscal Year: 2007
    ..These studies will provide a foundation for further studies of host-pathogen relationships in picornavirus disease and the potential utility of picornaviruses as enterally delivered vaccine vectors. ..
  16. Anti-HIV Therapeutics that Target HIV Nuclear Import
    Omar Haffar; Fiscal Year: 2002
    ..PROPOSED COMMERCIAL APPLICATION: Not Available ..
  17. STRUCTURE/FUNCTION OF HIV1 CAPSID/CYCLOPHILIN COMPLEX
    Wesley Sundquist; Fiscal Year: 2001
    ..Finally, genetic analysis of mutants of CA in an HIV provirus will be used to test models that arise from the CA and CA-cyclophilin structures. ..
  18. Regulation of APOBEC3G enzymatic activity in HIV-infected primary human T cells
    Jaisri R Lingappa; Fiscal Year: 2010
    ....