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Genomes and GenesSpecies | HHT2SummaryGene Symbol: HHT2 Description: Hht2p Species: Top Publications
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Acetylation in histone H3 globular domain regulates gene expression in yeastFeng Xu
Department of Biological Chemistry, Geffen School of Medicine at UCLA, and the Molecular Biology Institute, University of California Los Angeles, Boyer Hall, Los Angeles, CA 90095, USA
Cell 121:375-85. 2005..These findings indicate that histone H3 K56 acetylation at the entry-exit gate enables recruitment of the SWI/SNF nucleosome remodeling complex and so regulates gene activity...- The program for processing newly synthesized histones H3.1 and H4Eric I Campos
Howard Hughes Medical Institute, Department of Biochemistry, New York University School of Medicine, New York, New York, USA
Nat Struct Mol Biol 17:1343-51. 2010..We further demonstrate the high degree of conservation for this pathway between higher and lower eukaryotes... - Evidence that the localization of the elongation factor Spt16 across transcribed genes is dependent upon histone H3 integrity in Saccharomyces cerevisiaeAndrea A Duina
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
Genetics 177:101-12. 2007.... - The histone chaperone anti-silencing function 1 stimulates the acetylation of newly synthesized histone H3 in S-phaseMelissa W Adkins
Department of Biochemistry and Molecular Genetics, School of Medicine, University of Colorado, Aurora, Colorado 80045, USA
J Biol Chem 282:1334-40. 2007..These results demonstrate that Asf1 does not affect the stability of the newly synthesized histones per se, but instead histone binding by Asf1 promotes the efficient acetylation of specific residues of newly synthesized histone H3... - Synthetic lethal screens identify gene silencing processes in yeast and implicate the acetylated amino terminus of Sir3 in recognition of the nucleosome coreTibor van Welsem
The Netherlands Cancer Institute, Division of Cellular Biochemistry, Plesmanlaan 121, 1066CX Amsterdam, The Netherlands
Mol Cell Biol 28:3861-72. 2008..Our results suggest that the BAH domain of Sir3 binds to histone H3K79 and that acetylation of the BAH domain is required for the binding specificity of Sir3 for nucleosomes unmethylated at H3K79... - ASF1 binds to a heterodimer of histones H3 and H4: a two-step mechanism for the assembly of the H3-H4 heterotetramer on DNAChristine M English
Department of Biochemistry and Molecular Genetics, University of Colorado Health Sciences Center at Fitzsimons, Aurora, Colorado 80045, USA
Biochemistry 44:13673-82. 2005..We demonstrate that Asf1 blocks formation of the H3-H4 heterotetramer by a mechanism that likely involves occlusion of the H3-H3 dimerization interface... - Histone levels are regulated by phosphorylation and ubiquitylation-dependent proteolysisRakesh Kumar Singh
Department of Biomedical Sciences, College of Medicine, Florida State University, 1115 West Call Street, Tallahassee, FL 32306 4300, USA
Nat Cell Biol 11:925-33. 2009..Regulated histone proteolysis has major implications for the maintenance of epigenetic marks on chromatin, genomic stability and the packaging of sperm DNA... - Association with the origin recognition complex suggests a novel role for histone acetyltransferase Hat1p/Hat2pBernhard Suter
Program in Proteomics and Bioinformatics, Banting and Best Department of Medical Genetics, University of Toronto, Toronto, Ontario, Canada
BMC Biol 5:38. 2007..In yeast, a major conserved histone acetyltransferase, Hat1p, preferentially acetylates lysine residues 5 and 12 on histone H4... - Dot1p modulates silencing in yeast by methylation of the nucleosome coreFred van Leeuwen
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
Cell 109:745-56. 2002..Because Dot1p and histone H3 are conserved, similar mechanisms are likely at work in other eukaryotes... - Lysine methylation within the globular domain of histone H3 by Dot1 is important for telomeric silencing and Sir protein associationHuck Hui Ng
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
Genes Dev 16:1518-27. 2002..Our results indicate that histone modifications in the core globular domain have important biological functions... - Interplay of chromatin modifiers on a short basic patch of histone H4 tail defines the boundary of telomeric heterochromatinMohammed Altaf
Laval University Cancer Research Center, Hotel Dieu de Quebec, Centre Hospitalier Universitaire de Quebec, 9 McMahon Street, Quebec City, QC G1R 2J6, Canada
Mol Cell 28:1002-14. 2007..This draws a detailed picture of the succession of molecular events occurring during the establishment of telomeric heterochromatin boundaries... - A role for cell-cycle-regulated histone H3 lysine 56 acetylation in the DNA damage responseHiroshi Masumoto
Chromosome Dynamics Laboratory, Cancer Research UK, London Research Institute, Clare Hall Laboratories, Blanche Lane, South Mimms, Hertfordshire EN6 3LD, UK
Nature 436:294-8. 2005..We suggest that the acetylation of histone H3 K56 creates a favourable chromatin environment for DNA repair and that a key component of the DNA damage response is to preserve this acetylation... - A Rad53 kinase-dependent surveillance mechanism that regulates histone protein levels in S. cerevisiaeAkash Gunjan
Cancer Research UK, London Research Institute, Clare Hall Laboratories, Blanche Lane, South Mimms, Hertfordshire EN6 3LD, United Kingdom
Cell 115:537-49. 2003..Our results argue that Rad53 contributes to genome stability independently of Mec1 by preventing the damaging effects of excess histones both during normal cell cycle progression and in response to DNA damage... - Insights into the role of histone H3 and histone H4 core modifiable residues in Saccharomyces cerevisiaeEdel M Hyland
High Throughput Biology Center, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Mol Cell Biol 25:10060-70. 2005..Finally, we allude to the possible molecular mechanisms underlying the effects of these modifications... - Proteome-wide analysis in Saccharomyces cerevisiae identifies several PHD fingers as novel direct and selective binding modules of histone H3 methylated at either lysine 4 or lysine 36Xiaobing Shi
Department of Biological Sciences, Stanford University, Stanford, California 94305, USA
J Biol Chem 282:2450-5. 2007..Together, our study suggests that a common function for PHD fingers is to transduce methyl-lysine events and sheds light on how a single histone modification can be linked to multiple biological outcomes... - The LRS and SIN domains: two structurally equivalent but functionally distinct nucleosomal surfaces required for transcriptional silencingChristopher J Fry
Program in Molecular Medicine, University of Massachusetts Medical School, Biotech 2, Suite 210, Worcester, MA 01605, USA
Mol Cell Biol 26:9045-59. 2006..Our study shows that structurally similar nucleosomal surfaces provide distinct functionalities in vivo and in vitro... - Analysis of a mutant histone H3 that perturbs the association of Swi/Snf with chromatinAndrea A Duina
Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
Mol Cell Biol 24:561-72. 2004..Here we describe the characterization of one such mutant, encoded by the hht2-11 allele, which contains the single amino acid change L61W in the globular domain of H3... - Role of the conserved Sir3-BAH domain in nucleosome binding and silent chromatin assemblyMegumi Onishi
Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
Mol Cell 28:1015-28. 2007..Together, these observations suggest that the SIR complex associates with an extended chromatin fiber through interactions with two different regions in the nucleosome... - UV sensitive mutations in histone H3 in Saccharomyces cerevisiae that alter specific K79 methylation states genetically act through distinct DNA repair pathwaysMargery L Evans
Department of Biology, Denison University, Granville, OH 43023, USA
Curr Genet 53:259-74. 2008.... - The structure of the yFACT Pob3-M domain, its interaction with the DNA replication factor RPA, and a potential role in nucleosome depositionAndrew P VanDemark
Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, 84132, USA
Mol Cell 22:363-74. 2006..These results support the model that the FACT family has an essential role in constructing nucleosomes during DNA replication, and suggest that RPA contributes to this process... - Expanded lysine acetylation specificity of Gcn5 in native complexesP A Grant
Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 16802 4500, USA
J Biol Chem 274:5895-900. 1999..Furthermore Ada and SAGA have overlapping, yet distinct, patterns of acetylation, suggesting that the association of specific subunits determines site specificity... - Regulation of H3K4 trimethylation via Cps40 (Spp1) of COMPASS is monoubiquitination independent: implication for a Phe/Tyr switch by the catalytic domain of Set1Yoh hei Takahashi
Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, MO 64110, USA
Mol Cell Biol 29:3478-86. 2009..Our studies provide a molecular basis for the way in which H3K4 trimethylation is regulated by Tyr1052 and the Cps40 subunit of COMPASS... - Disruptor of telomeric silencing-1 is a chromatin-specific histone H3 methyltransferaseNicolas Lacoste
Laval University Cancer Research Center, Hôtel Dieu de Québec CHUQ, 9 McMahon Street, Quebec City, Quebec G1R 2J6, Canada
J Biol Chem 277:30421-4. 2002..In agreement with a role in regulating localization of histone deacetylase complexes like SIR, an increase of bulk histone acetylation is detected in dot1- cells... - Dimethylation of H3K4 by Set1 recruits the Set3 histone deacetylase complex to 5' transcribed regionsTaesoo Kim
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
Cell 137:259-72. 2009.... - Chz1, a nuclear chaperone for histone H2AZEd Luk
Laboratory of Biochemistry and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
Mol Cell 25:357-68. 2007..The presence of this motif in other metazoan proteins suggests that H2AZ-specific chaperones may be widely conserved... - Identification of Rkr1, a nuclear RING domain protein with functional connections to chromatin modification in Saccharomyces cerevisiaeMary A Braun
Department of Biological Sciences, University of Pittsburgh, 269 Crawford Hall, 4249 Fifth Avenue, Pittsburgh, PA 15260, USA
Mol Cell Biol 27:2800-11. 2007..Taken together, our results identify a new participant in a protein ubiquitylation pathway within the nucleus that acts to modulate chromatin function and transcription... - Two Saccharomyces cerevisiae JmjC domain proteins demethylate histone H3 Lys36 in transcribed regions to promote elongationTaesoo Kim
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
J Biol Chem 282:20827-35. 2007..Taken together, these findings indicate that a general function of histone demethylases for H3 Lys(36) is to promote transcription elongation by antagonizing repressive Lys(36) methylation by Set2... - A novel histone acetyltransferase inhibitor modulating Gcn5 network: cyclopentylidene-[4-(4'-chlorophenyl)thiazol-2-yl)hydrazoneFranco Chimenti
Dipartimento di Chimica e Tecnologie del Farmaco, Universita degli Studi di Roma La Sapienza, P le A Moro 5, 00185 Rome, Italy
J Med Chem 52:530-6. 2009..In vivo, it decreased acetylation of bulk histone H3 at the specific H3-AcK14 site. On the whole, our results demonstrate that CPTH2 is a novel HAT inhibitor modulating Gcn5p network in vitro and in vivo... - H3 k36 methylation helps determine the timing of cdc45 association with replication originsFiona Pryde
Wellcome Trust Centre for Cell Biology, Institute of Cell Biology, University of Edinburgh, Edinburgh, United Kingdom
PLoS ONE 4:e5882. 2009..A pivotal step during this conversion is the binding of the replication factor Cdc45, which associates with replication origins at approximately their time of activation in a manner partially controlled by histone acetylation... - The RNA polymerase II kinase Ctk1 regulates positioning of a 5' histone methylation boundary along genesTiaojiang Xiao
Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, 405 Mary Ellen Jones, Chapel Hill, NC 27599 7260, USA
Mol Cell Biol 27:721-31. 2007.... - Selective recognition of acetylated histones by bromodomains in transcriptional co-activatorsAhmed H Hassan
Department of Biochemistry, Faculty of Medicine and Health Sciences, UAE University, P O Box 17666, Al Ain, United Arab Emirates
Biochem J 402:125-33. 2007..The selective recognition of the bromodomains observed in the present study accounts for the broad effects of bromodomain-containing proteins observed on binding to histones... - Histone H3 Lys79 methylation is required for efficient nucleotide excision repair in a silenced locus of Saccharomyces cerevisiaeShubho Chaudhuri
Biochemistry and Biophysics, School of Molecular Biosciences, Washington State University, Pullman, WA 99164 4660, USA
Nucleic Acids Res 37:1690-700. 2009..These results indicate that impaired NER occurs in the silenced chromatin of H3K79R and H3K4,79R mutants as a result of increased binding of Sir complexes, which may reduce DNA lesion accessibility to repair enzymes... - Compensatory interactions between Sir3p and the nucleosomal LRS surface imply their direct interactionAnne Norris
Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD, USA
PLoS Genet 4:e1000301. 2008..Based on these findings, we propose an electrostatic model for how an extensive surface on the Sir3p BAH domain may regulate docking onto the LRS surface... - Saccharomyces cerevisiae Yta7 regulates histone gene expressionAngeline Gradolatto
Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA
Genetics 179:291-304. 2008..Our work suggests a mechanism in which Yta7 is localized to chromatin to establish regions of transcriptional silencing, and that one facet of this cellular mechanism is to modulate transcription of histone genes... - Acetylation of histone H3 lysine 56 regulates replication-coupled nucleosome assemblyQing Li
Department of Biochemistry and Molecular Biology, Mayo Clinic, College of Medicine, 200 First Street SW, Rochester, MN 55905, USA
Cell 134:244-55. 2008..These results reveal a mechanism by which H3K56Ac regulates replication-coupled nucleosome assembly mediated by CAF-1 and Rtt106... - Hst3 is regulated by Mec1-dependent proteolysis and controls the S phase checkpoint and sister chromatid cohesion by deacetylating histone H3 at lysine 56Safia Thaminy
Clinical Research Division and Human Biology Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
J Biol Chem 282:37805-14. 2007..Both S phase checkpoint and SCC defects are phenocopied by H3K56 point mutants. Our findings demonstrate that Hst3-regulated H3K56 acetylation safeguards genome stability by controlling the S phase DNA damage response and promoting SCC... - Structural insights into histone H3 lysine 56 acetylation by Rtt109Chengqi Lin
Genome and Structural Biology Program, Temasek Life Sciences Laboratory and Department of Biological Sciences, National University of Singapore, 1 Research Link, Singapore 117604, Singapore
Structure 16:1503-10. 2008..We have further proposed the unique H3-K56 anchoring pocket and the potential H3alphaN binding groove. Our work has provided structural insights to understand the acetylation mechanism of H3-K56 by Rtt109... - Critical determinants for chromatin binding by Saccharomyces cerevisiae Yng1 exist outside of the plant homeodomain fingerAdam Chruscicki
Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
Genetics 185:469-77. 2010..Although these motifs can bind histones independently, together they increase the apparent association of Yng1 for the H3 tail... - Origin recognition complex (ORC) mediates histone 3 lysine 4 methylation through cooperation with Spp1 in Saccharomyces cerevisiaeJunsuo Kan
Department of Biochemistry and Center for Cancer Research, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
J Biol Chem 283:33803-7. 2008..Our data reveal a novel facet of ORC in mediating histone methylation in collaboration with Spp1p and demonstrate a connection between ORC and chromatin structure via the Set1 complex... - Histone H3 K36 methylation is mediated by a trans-histone methylation pathway involving an interaction between Set2 and histone H4Hai Ning Du
Department of Biochemistry and Purdue Cancer Center, Purdue University, West Lafayette, Indiana 47907, USA
Genes Dev 22:2786-98. 2008..Overall, our study defines a new interaction between Set2 and histone H4 that mediates trans-histone regulation of H3 K36 methylation, which is needed for the preventative maintenance and integrity of the genome... - Swi3p controls SWI/SNF assembly and ATP-dependent H2A-H2B displacementXiaofang Yang
Interdisciplinary Graduate Program, Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
Nat Struct Mol Biol 14:540-7. 2007..Our data indicate that H2A-H2B dimer loss is not an obligatory consequence of ATP-dependent DNA translocation, and furthermore they suggest that SWI/SNF is composed of at least four interdependent modules... - Proline isomerization of histone H3 regulates lysine methylation and gene expressionChristopher J Nelson
Gurdon Institute and Department of Pathology, Tennis Court Road, Cambridge, CB2 1QR, UK
Cell 126:905-16. 2006..These results identify proline isomerization as a novel noncovalent histone modification that regulates transcription and provides evidence for crosstalk between histone lysine methylation and proline isomerization... - Genetic interactions between POB3 and the acetylation of newly synthesized histonesDevi M Nair
Department of Molecular and Cellular Biochemistry, The Ohio State University, Columbus, OH 43210, USA
Curr Genet 57:271-86. 2011..These results support the model that the yFACT complex plays a central role in chromatin assembly pathways regulated by acetylation of newly synthesized histones... - Crystal structure of the catalytic core of Saccharomyces cerevesiae histone demethylase Rph1: insights into the substrate specificity and catalytic mechanismYuanyuan Chang
Research Center for Structural Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, China
Biochem J 433:295-302. 2011..Taken together, our structural and biological data provide insights into the molecular basis of the histone demethylase activity and the substrate specificity of Rph1... - Dimethylation of histone H3 at lysine 36 demarcates regulatory and nonregulatory chromatin genome-wideBhargavi Rao
Department of Biology, CB no 3280, 203 Fordham Hall, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
Mol Cell Biol 25:9447-59. 2005.... - Genetic interaction of RAD53 protein kinase with histones is important for DNA replicationTeresa M Holzen
Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, Aurora, CO, USA
Cell Cycle 9:4735-47. 2010..We propose a model in which Rad53 acts as a "nucleosome buffer," interacting with origins of replication to prevent the binding of excess histones to origin DNA and to maintain proper chromatin configuration... - Dynamic histone acetylation is critical for cotranscriptional spliceosome assembly and spliceosomal rearrangementsFelizza Q Gunderson
Department of Microbiology and Immunology, Northwestern University, Chicago, IL 60611, USA
Proc Natl Acad Sci U S A 108:2004-9. 2011..These studies show that cotranscriptional spliceosome rearrangements are driven by dynamic changes in the acetylation state of histones and provide a model whereby yeast spliceosome assembly is tightly coupled to histone modification... - Identification of a novel allele of SIR3 defective in the maintenance, but not the establishment, of silencing in Saccharomyces cerevisiaeS Enomoto
Department of Genetics, Cell Biology and Development, University of Minnesota, St Paul 55108, USA
Genetics 155:523-38. 2000..Furthermore, HM silencing is most vulnerable to disruption by the Sir3-P898R C terminus immediately after S-phase, the time when new silent chromatin is assembled onto newly replicated DNA... - A role for histone H4K16 hypoacetylation in Saccharomyces cerevisiae kinetochore functionJohn S Choy
Genetics Branch Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
Genetics 189:11-21. 2011..Our work shows that hypoacetylated centromeric H4 is conserved across eukaryotic centromeres and hypoacetylation of H4K16 at centromeres plays an important role in accurate chromosome segregation... - Histone H3 lysine 4 hypermethylation prevents aberrant nucleosome remodeling at the PHO5 promoterShan Shan Wang
State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031, China
Mol Cell Biol 31:3171-81. 2011..We propose that the restriction of aberrant nucleosome remodeling contributes to strict control of gene transcription by the transactivators... - Lsm1 promotes genomic stability by controlling histone mRNA decayAna B Herrero
Instituto de Biologia Molecular y Celular del Cancer, CSIC Universidad de Salamanca, Campus Miguel de Unamuno, Salamanca, Spain
EMBO J 30:2008-18. 2011..Our results demonstrate that improper histone stoichiometry leads to genomic instability and highlight the importance of regulating histone mRNA decay in the tight control of histone levels in yeast... - Sterile 20 kinase phosphorylates histone H2B at serine 10 during hydrogen peroxide-induced apoptosis in S. cerevisiaeSung Hee Ahn
Laboratory of Chromatin Biology, The Rockefeller University, Box 78, New York, NY 10021, USA
Cell 120:25-36. 2005.... - Structural basis for the role of the Sir3 AAA+ domain in silencing: interaction with Sir4 and unmethylated histone H3K79Stefan Ehrentraut
Abteilung fur Genetik, Zentrum für Medizinische Biotechnologie ZMB, Universitat Duisburg Essen, D 45141 Essen, Germany
Genes Dev 25:1835-46. 2011..In summary, the unique folding of this conserved Sir3 AAA(+) domain generates novel surface regions that mediate Sir3-Sir4 and Sir3-nucleosome interactions, both being required for the proper assembly of heterochromatin in living cells... - The requirements for COMPASS and Paf1 in transcriptional silencing and methylation of histone H3 in Saccharomyces cerevisiaeJohn E Mueller
Department of Biochemistry and Biophysics, Texas A and M University, College Station, Texas 77843 2128, USA
Genetics 173:557-67. 2006..Finally, we show Paf1 is required for silencing and K4-methylated H3 at the rDNA, suggesting a possible direct role for K4-methylated H3 in gene silencing... - Transcription of chromosomal rRNA genes by both RNA polymerase I and II in yeast uaf30 mutants lacking the 30 kDa subunit of transcription factor UAFI N Siddiqi
University of California, Irvine, Department of Biological Chemistry, Irvine, CA 92697 1700, USA
EMBO J 20:4512-21. 2001..Thus, Uaf30p plays only a minor role in its activator function. Possible reasons for slow growth caused by uaf30 mutations are discussed... - Essential and redundant functions of histone acetylation revealed by mutation of target lysines and loss of the Gcn5p acetyltransferaseW Zhang
Department of Biochemistry and Molecular Biology, U T M D Anderson Cancer Center, Houston, TX 77030, USA
EMBO J 17:3155-67. 1998.... - Histone sumoylation is a negative regulator in Saccharomyces cerevisiae and shows dynamic interplay with positive-acting histone modificationsDafna Nathan
Gene Expression and Regulation Program, The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
Genes Dev 20:966-76. 2006..These results indicate that sumoylation is the first negative histone modification to be identified in S. cerevisiae and further suggest that sumoylation may serve as a general dynamic mark to oppose transcription... - Isw1 functions in parallel with the NuA4 and Swr1 complexes in stress-induced gene repressionKimberly C Lindstrom
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
Mol Cell Biol 26:6117-29. 2006..In contrast to a recruitment-based model, we find that the NuA4 and Swr1 complexes act throughout the genome while only a specific subset of the genome shows alterations in transcription... - ING2 PHD domain links histone H3 lysine 4 methylation to active gene repressionXiaobing Shi
Department of Biological Sciences, Stanford University, Stanford, California 94305, USA
Nature 442:96-9. 2006..Together, our findings establish a pivotal role for trimethylation of H3K4 in gene repression and, potentially, tumour suppressor mechanisms... - An evolutionarily 'young' lysine residue in histone H3 attenuates transcriptional output in Saccharomyces cerevisiaeEdel M Hyland
High Throughput Biology Center, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
Genes Dev 25:1306-19. 2011..Interestingly, this site of methylation is unique to Ascomycota, suggesting a recent evolutionary innovation that highlights the evolvability of post-translational modifications of chromatin... - Different roles of histone H3 lysine 4 methylation in chromatin maintenanceJa Hwan Seol
College of Pharmacy Sungkyunkwan University, 300 Cheoncheon dong, Jangan gu, Suwon, Gyeonggi do 440 746, South Korea
Biochem Biophys Res Commun 349:463-70. 2006..The mono-methylation of H3 K4 might be required for the global integrity of chromatin structure, which is normally monitored by the Rad53 dependent chromatin surveillance system... - The Gcn5.Ada complex potentiates the histone acetyltransferase activity of Gcn5P Syntichaki
Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology, Heraklion, 711 10 Crete, Greece
J Biol Chem 273:24414-9. 1998..Because Ada2 is required for the assembly of Gcn5, we conclude that one role for components of the Gcn5.Ada complex is the potentiation of its HAT activity... - A negatively charged residue in place of histone H3K56 supports chromatin assembly factor association but not genotoxic stress resistanceJudith A Erkmann
Program in Gene Function and Expression, University of Massachusetts Medical School, 364 Plantation St 506, Worcester, MA 01605, USA
DNA Repair (Amst) 8:1371-9. 2009..The suppressor is a point mutation in the histone H3 gene HHT2, and converts lysine 56 to glutamic acid... - Reconstitution of heterochromatin-dependent transcriptional gene silencingAaron Johnson
Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
Mol Cell 35:769-81. 2009..These results define a minimal set of components that mediate heterochromatic gene silencing and demonstrate distinct contributions for histone deacetylation and nucleosome binding in the silencing mechanism... - Cse4 is part of an octameric nucleosome in budding yeastRaymond Camahort
Stowers Institute for Medical Research, Kansas City, MO 64110, USA
Mol Cell 35:794-805. 2009..Taken together, our experimental evidence supports the model that the Cse4 nucleosome is an octamer, containing two copies each of Cse4, H2A, H2B, and H4... - Ubiquitylation of the COMPASS component Swd2 links H2B ubiquitylation to H3K4 trimethylationAdeline Vitaliano-Prunier
Institut Jacques Monod, Universite Paris VII, Universite Paris VI, CNRS, 2 place Jussieu, Tour 43, 75251 Paris Cedex 05, France
Nat Cell Biol 10:1365-71. 2008..Our results further indicate that Swd2 is a major H3-binding component of COMPASS. Swd2 thus represents a key factor that mediates crosstalk between H2B ubiquitylation and H3K4 trimethylation on chromatin... - Transcriptional silencing and longevity protein Sir2 is an NAD-dependent histone deacetylaseS Imai
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA
Nature 403:795-800. 2000..These findings provide a molecular framework of NAD-dependent histone deacetylation that connects metabolism, genomic silencing and ageing in yeast and, perhaps, in higher eukaryotes... - Esa1p is an essential histone acetyltransferase required for cell cycle progressionA S Clarke
Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Colorado 80309 0347, USA
Mol Cell Biol 19:2515-26. 1999..These observations therefore link an essential HAT activity to cell cycle progression, potentially through discrete transcriptional regulatory events... - Site specificity of yeast histone acetyltransferase B complex in vivoAna Poveda
Departament de Bioquimica i Biologia Molecular, Universitat de Valencia, Spain
FEBS J 275:2122-36. 2008..Finally, the exchange of Lys for Arg at position 12 of histone H4 did not interfere with histone H4 association with the complex, but prevented acetylation on Lys5 by the HAT-B enzyme, in vivo as well as in vitro... - Mutational analysis of the Sir3 BAH domain reveals multiple points of interaction with nucleosomesVinaya Sampath
Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, NY 11794 5215, USA
Mol Cell Biol 29:2532-45. 2009..These results, together with the previously characterized interaction between the C-terminal region of Sir3 and the histone H3/H4 tails, suggest that Sir3 utilizes multiple domains to interact with nucleosomes... - Histone chaperone specificity in Rtt109 activationYoung Jun Park
Howard Hughes Medical Institute, Colorado State University, Fort Collins, Colorado 80523 1870, USA
Nat Struct Mol Biol 15:957-64. 2008..Nap1 and Vps75 interact with histones and Rtt109 with comparable affinities. However, only Vps75 stimulates Rtt109 enzymatic activity. Our data highlight the functional specificity of Vps75 in Rtt109 activation... - The Gcn5 bromodomain of the SAGA complex facilitates cooperative and cross-tail acetylation of nucleosomesShanshan Li
Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, Iowa 50011, USA
J Biol Chem 284:9411-7. 2009.... - Distinct transcriptional outputs associated with mono- and dimethylated histone H3 arginine 2Antonis Kirmizis
Gurdon Institute and Department of Pathology, Cambridge CB2 1QN, UK
Nat Struct Mol Biol 16:449-51. 2009..Collectively, these results indicate that different H3R2 methylation states have defined roles in gene expression... - Proliferating cell nuclear antigen and ASF1 modulate silent chromatin in Saccharomyces cerevisiae via lysine 56 on histone H3Andrew Miller
Department of Biochemistry and Purdue Cancer Center, Purdue University, West Lafayette, Indiana 47907, USA
Genetics 179:793-809. 2008..We show that this defect, in turn, contributes to misregulation of epigenetic processes as well as of cellular responses to DNA damage... - A cryptic unstable transcript mediates transcriptional trans-silencing of the Ty1 retrotransposon in S. cerevisiaeJulia Berretta
Centre de Genetique Moleculaire Centre National de la Recherche Scientifique CGM CNRS, 91198 Gif Yvette, France
Genes Dev 22:615-26. 2008..Our results show the first example of an RNA-dependent gene trans-silencing mediated by epigenetic marks in S. cerevisiae... - The Yng1p plant homeodomain finger is a methyl-histone binding module that recognizes lysine 4-methylated histone H3David G E Martin
Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
Mol Cell Biol 26:7871-9. 2006..These results identify a novel function for the Yng1p PHD finger in promoting stabilization of the NuA3 complex at chromatin through recognition of histone H3 lysine 4 methylation... - Structure of the yeast histone H3-ASF1 interaction: implications for chaperone mechanism, species-specific interactions, and epigeneticsAndrew J Antczak
Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA
BMC Struct Biol 6:26. 2006..Asf1 is known to directly bind histone H3, however, high-resolution structural information about the geometry of this interaction was previously unknown... - Rtt109 acetylates histone H3 lysine 56 and functions in DNA replicationJunhong Han
Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, 200 First Street SW, Rochester, MN 55905, USA
Science 315:653-5. 2007..Thus, Rtt109, which shares no sequence homology with any other known HATs, is a unique HAT that acetylates H3-K56... - A feed-forward repression mechanism anchors the Sin3/histone deacetylase and N-CoR/SMRT corepressors on chromatinMichiel Vermeulen
NCMLS, Radboud University Nijmegen, Department of Molecular Biology, Geert Grooteplein Zuid 30, Nijmegen, The Netherlands
Mol Cell Biol 26:5226-36. 2006.... - Structure and function of the histone chaperone CIA/ASF1 complexed with histones H3 and H4Ryo Natsume
Japan Biological Information Research Centre JBIRC, Japan Biological Informatics Consortium JBIC, 2 42 Aomi, Koto ku, Tokyo 135 0064, Japan
Nature 446:338-41. 2007.... - Yeast Jhd2p is a histone H3 Lys4 trimethyl demethylaseGaoyang Liang
Howard Hughes Medical Institute, Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7295, USA
Nat Struct Mol Biol 14:243-5. 2007..Here we identify a novel budding yeast JmjC domain-containing H3-K4 demethylase, Jhd2p, that antagonizes the trimethyl modification state and contributes to regulation of telomeric silencing... - Yeast Rtt109 promotes genome stability by acetylating histone H3 on lysine 56Robert Driscoll
Wellcome Trust and Cancer Research U K Gurdon Institute and the Department of Zoology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK
Science 315:649-52. 2007..These data establish Rtt109p as a member of a new class of histone acetyltransferases and show that its actions are critical for cell survival in the presence of DNA damage during S phase... - The BUR1 cyclin-dependent protein kinase is required for the normal pattern of histone methylation by SET2Yaya Chu
Department of Molecular Genetics, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
Mol Cell Biol 26:3029-38. 2006..These results establish links between a regulatory protein kinase and histone methylation and lead to a model in which the Bur1-Bur2 complex counteracts an inhibitory effect of Set2-dependent histone methylation... - Mutational analysis of H3 and H4 N termini reveals distinct roles in nuclear importJeffrey S Blackwell
Center for Cell Signaling, Department of Microbiology, University of Virginia Health Sciences Center, University of Virginia, Charlottesville, VA 22908, USA
J Biol Chem 282:20142-50. 2007..Acetylation may be important for modulating the interaction with transport factors and may play a role in the release of histones from karyopherins in the nucleus... - Contribution of Trf4/5 and the nuclear exosome to genome stability through regulation of histone mRNA levels in Saccharomyces cerevisiaeClara C Reis
Braun Laboratories, California Institute of Technology, Pasadena, California 91125, USA
Genetics 175:993-1010. 2007..These results identify TRF4, TRF5, and RRP6 as new players in the regulation of histone mRNA levels in yeast. To our knowledge, the histone transcripts are the first mRNAs that are upregulated in Trf mutants... - Swc2 is a widely conserved H2AZ-binding module essential for ATP-dependent histone exchangeWei Hua Wu
Laboratory of Molecular Cell Biology, Center for Cancer Research, National Cancer Institute, US National Institutes of Health, Building 37, Room 6068, Bethesda, Maryland 20892 4255, USA
Nat Struct Mol Biol 12:1064-71. 2005..Finally, the C-terminal alpha-helix of H2AZ is crucial for its recognition by SWR1. These findings provide insight on the initial events of histone exchange... - Sir2 deacetylates histone H3 lysine 56 to regulate telomeric heterochromatin structure in yeastFeng Xu
Department of Biological Chemistry, Geffen School of Medicine at UCLA, and the Molecular Biology Institute, Boyer Hall, 611 Young Drive East, Los Angeles, CA 90095, USA
Mol Cell 27:890-900. 2007..Since the spread of Sir proteins is necessary but not sufficient for silencing, we propose that silencing occurs when Sir2 deacetylates H3 K56 to close the nucleosomal entry-exit gates, enabling compaction of heterochromatin... - Histone chaperones regulate histone exchange during transcriptionHye Jin Kim
College of Pharmacy, Sungkyunkwan University, Suwon, Gyeonggi Do, Republic of Korea
EMBO J 26:4467-74. 2007..While Asf1 mediated incorporation of external H3/H4 and renewal of pre-existing histones, HIR opposed it. The balance of two opposing activities might be an important mechanism for determining current chromatin states... - Rtt106p is a histone chaperone involved in heterochromatin-mediated silencingShengbing Huang
Department of Biochemistry and Molecular Biology, Mayo Clinic, College of Medicine, 200 First Street SW, Rochester, MN 55905, USA
Proc Natl Acad Sci U S A 102:13410-5. 2005..Furthermore, Rtt106p interacts with CAF-1 physically through Cac1p. These biochemical and genetic data indicate that Rtt106p is a previously uncharacterized histone chaperone connecting S phase to epigenetic inheritance... - Characterising the binding specificities of the subunits associated with the KMT2/Set1 histone lysine methyltransferaseBen L Murton
MRC Cancer Cell Unit, Hutchison MRC Research Centre, Cambridge CB2 0XZ, UK
J Mol Biol 398:481-8. 2010..These data allow us to suggest a mechanism for the regulation of COMPASS activity at an actively transcribed gene... - Ybp2 associates with the central kinetochore of Saccharomyces cerevisiae and mediates proper mitotic progressionKentaro Ohkuni
Department of Molecular Pharmacology, St Jude Children s Research Hospital, Memphis, Tennessee, USA
PLoS ONE 3:e1617. 2008..Ybp2 seems to be part of a macromolecular kinetochore complex and appears to contribute to the proper associations among the central kinetochore subcomplexes and the kinetochore-specific nucleosome... - Chromatin-modifiying enzymes are essential when the Saccharomyces cerevisiae morphogenesis checkpoint is constitutively activatedMyriam Ruault
Division of Biological Sciences, UCSD Moores Cancer Center, University of California, San Diego, California 92093 0347, USA
Genetics 174:1135-49. 2006..A catalytically dead Hsl7p retained wild-type interactions, implying that modification of histone H3 or H4 N termini by Gcn5p, Esa1p, Rpd3p, and Set1p, but not by Hsl7p, was needed to bypass the morphogenesis checkpoint... - Pathways mediating the nuclear import of histones H3 and H4 in yeastNima Mosammaparast
Center for Cell Signaling, Department of Microbiology, University of Virginia Health Sciences Center, University of Virginia, Charlottesville, Virginia 22908, USA
J Biol Chem 277:862-8. 2002..H3 and H4 are the first histones to be assembled onto DNA, and these results show that their import is mediated by at least two import pathways... - Sir3-nucleosome interactions in spreading of silent chromatin in Saccharomyces cerevisiaeJohannes R Buchberger
Howard Hughes Medical Institute, Harvard Medical School, 240 Longwood Ave, Boston, MA 02115, USA
Mol Cell Biol 28:6903-18. 2008..We propose a model for the spreading of the SIR complex along the chromatin fiber through the two distinct histone-binding domains in Sir3... - Sth1p, a Saccharomyces cerevisiae Snf2p/Swi2p homolog, is an essential ATPase in RSC and differs from Snf/Swi in its interactions with histones and chromatin-associated proteinsJ Du
Department of Microbiology and Immunology and Morse Institute for Molecular Genetics, State University of New York, Brooklyn, New York 11203, USA
Genetics 150:987-1005. 1998..These results provide a framework for understanding the ATP-dependent RSC function in modeling chromatin and its connection to the cell cycle... - The bromodomain of Gcn5p interacts in vitro with specific residues in the N terminus of histone H4P Ornaghi
Centro di Studio per gli Acidi Nucleici, CNR, Dipartimento di Genetica e Biologia Molecolare, Universita La Sapienza, P le A Moro n degrees 5, Roma, 00185, Italy
J Mol Biol 287:1-7. 1999..This evidence and the known dispensability of the bromodomain for Gcn5p acetyltransferase activity suggest a new structural role for the highly evolutionary conserved bromodomain... - Dynamic interaction of DNA damage checkpoint protein Rad53 with chromatin assembly factor Asf1A Emili
Division of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
Mol Cell 7:13-20. 2001..Biochemical and molecular genetic studies suggest that Asf1 is an important target of the Rad53-dependent DNA damage response and that Rad53 may directly regulate chromatin assembly during DNA replication and repair... - Sir3-dependent assembly of supramolecular chromatin structures in vitroP T Georgel
Department of Biochemistry, University of Texas Health Science Center, San Antonio, TX 78229 3900, USA
Proc Natl Acad Sci U S A 98:8584-9. 2001..Based on these data we hypothesize that Sir3p functions, at least in part, by mediating reorganization of the canonical chromatin fiber into functionally specialized higher order chromosomal domains... - The Snf1 protein kinase and Sit4 protein phosphatase have opposing functions in regulating TATA-binding protein association with the Saccharomyces cerevisiae INO1 promoterMargaret K Shirra
Department of Biological Sciences, University of Pittsburgh, Pennsylvania 15260, USA
Genetics 169:1957-72. 2005..However, analysis of rapamycin-treated cells suggests that Sit4 represses INO1 transcription through multiple mechanisms, only one of which may involve inhibition of TOR signaling... - Characterization of lysine 56 of histone H3 as an acetylation site in Saccharomyces cerevisiaeAnil Ozdemir
Department of Molecular Biology, Nijmegen Center for Molecular Life Sciences, Radboud University, 6500 HB Nijmegen, The Netherlands
J Biol Chem 280:25949-52. 2005..Finally, reverse genetic analysis indicates that none of the known histone acetyltransferases is solely responsible for H3-K56 acetylation in Saccharomyces cerevisiae...
Research Grants
- THE ROLE OF HISTONE H4 IN GENOME STABILITYM Smith; Fiscal Year: 2003..Our observation that reversible histone H4 acetylation is required for DNA repair represents a previously unrecognized role for this important chromatin modification. ..
- THE ROLE OF HISTONE H4 IN GENOME STABILITYM Smith; Fiscal Year: 2007..abstract_text> ..
- HISTONE GENE EXPRESSION IN YEASTM Smith; Fiscal Year: 2007..To understand the rules and mechanisms for these assembly patterns, we will engineer mutations in specific cis- and trans-acting factors to test alternative models of Htz1 recruitment. ..
- Epigenetic Regulation of Gene Expression During Early Mouse EmbryogenesisM Smith; Fiscal Year: 2007..The results of these studies will have a significant impact on how we think about, and manage, a wide range of human health issues including assisted reproductive techniques, somatic nuclear transfer, and stem cell therapy. ..
- HISTONE GENE EXPRESSION IN YEASTM Smith; Fiscal Year: 1999..We propose that histone H4 and CENP-A form a specialized nucleosome at the centromere and interact with other kinetochore proteins. This model will be tested both genetically and biochemically. ..
- HISTONE GENE EXPRESSION IN YEASTM Smith; Fiscal Year: 1991..Both the transcription of these related genes and the chromatin structure of the mating type genes will be assayed...
- HISTONE GENE EXPRESSION IN YEASTM Smith; Fiscal Year: 1993..A mutational analysis of the UAS binding protein will be carried out to determine its molecular function, and define the signal transduction pathway for cell cycle position information...
- The Role of MYST Histone Acetyltransferase in Genome StabilityM Mitchell Smith; Fiscal Year: 2010..Little is known about their range of functions and target pathways. The research proposed in this application is designed to uncover new principles in how these enzymes work, what they do, and how they do it. ..