Rtt107/Esc4 binds silent chromatin and DNA repair proteins using different BRCT motifsDavid C Zappulla
Department of Biochemistry and Cellular Biology, Stony Brook University, Stony Brook, NY 11794, USA
BMC Mol Biol 7:40. 2006
..In this study we aimed to determine the mechanism of Rtt107/Esc4 targeted silencing and also learn more about its biological functions...
Esc1, a nuclear periphery protein required for Sir4-based plasmid anchoring and partitioningErik D Andrulis
Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, New York 11794 5215, USA
Mol Cell Biol 22:8292-301. 2002
..Our data suggest that Esc1 is a component of a redundant pathway that functions to localize silencing complexes to the nuclear periphery...
One-hybrid screens at the Saccharomyces cerevisiae HMR locus identify novel transcriptional silencing factorsErik D Andrulis
Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, New York 11794, USA
Genetics 166:631-5. 2004
..Here, we report results of screening a Gal4 DNA-binding domain hybrid library for proteins that cause silencing when targeted to a silencer-defective HMR locus...
Control of replication timing by a transcriptional silencerDavid C Zappulla
Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, NY 11794, USA
Curr Biol 12:869-75. 2002
..Presumably, the tight chromatin structure promoted by Sir proteins favors both transcriptional inactivity and late replication...
Yeast telomerase RNA: a flexible scaffold for protein subunitsDavid C Zappulla
Howard Hughes Medical Institute and Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO 80309-0215, USA
Proc Natl Acad Sci U S A 101:10024-9. 2004
..In the case of yeast telomerase, we propose that the RNA serves a very different function, providing a flexible tether for the protein subunits...
A miniature yeast telomerase RNA functions in vivo and reconstitutes activity in vitroDavid C Zappulla
Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309-0215, USA
Nat Struct Mol Biol 12:1072-7. 2005
..We find that substitution of Mini-T for wild-type TLC1 in a reconstituted system yields robust activity, allowing the contributions of individual yeast telomerase components to be directly assessed...