Genetic mapping of the duffy binding protein (DBP) ligand domain of Plasmodium vivax from unstable malaria region in the Middle EastLaleh Babaeekho
Malaria and Vector Research Group MVRG, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
Am J Trop Med Hyg 80:112-8. 2009
..It is suggested that to develop polyvalent vaccine against P. vivax infection, it is better to incorporate the common and high prevalent allelic variants of the antigen that were reported from different malaria endemic regions...
- In vitro and in vivo anti-malarial activity of Boerhavia elegans and Solanum surattense
Ali Ramazani
Malaria and Vector Research Group MVRG, Biotechnology Research Center, Institut Pasteur of Iran, Tehran, Iran
Malar J 9:124. 2010
..In the present study, the aim was to discover novel, effective plant-based extracts for the activity against malaria...
- Molecular surveillance of Plasmodium vivax dhfr and dhps mutations in isolates from Afghanistan
Sedigheh Zakeri
Malaria and Vector Research Group, Biotechnology Research Center, Institut Pasteur of Iran, Pasteur Avenue, PO BOX 1316943551, Tehran, Iran
Malar J 9:75. 2010
..The present study investigates the frequency of SNPs-haplotypes in the dhfr and dhps genes in P. vivax clinical isolates circulating in two malaria endemic areas in Afghanistan...
- Plasmodium vivax: prevalence of mutations associated with sulfadoxine-pyrimethamine resistance in Plasmodium vivax clinical isolates from Pakistan
Sedigheh Zakeri
Malaria and Vector Research Group, Biotechnology Research Center, Pasteur Institute of Iran, Pasteur Avenue, PO BOX 1316943551, Tehran, Iran
Exp Parasitol 127:167-72. 2011
..6%) and I(13)P(33)F(57)R(58)T(61)N(117)I(173)/A(383)A(553) (12.2%). The presence of mutant haplotypes is worrying and indicates the emergence of drug tolerant/resistant P. vivax isolates in Pakistan in near future...
- Association of pfcrt but not pfmdr1 alleles with chloroquine resistance in Iranian isolates of Plasmodium falciparum
Sedigheh Zakeri
Malaria and Vector Research Group, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
Am J Trop Med Hyg 78:633-40. 2008
..falciparum mutations associated with resistance to CQ are abundant in south-eastern Iran and this finding strongly supports that CQ as the first line drug is inadequate for treatment of uncomplicated falciparum malaria in Iran...
- Genetic diversity of transmission blocking vaccine candidate (Pvs25 and Pvs28) antigen in Plasmodium vivax clinical isolates from Iran
Sedigheh Zakeri
Malaria and Vector Research Group MVRG, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
Acta Trop 109:176-80. 2009
..These results highly encourage with respect to applicability of Pvs25 and Pvs28-based vaccine against P. vivax infection in the region, where these parasites are prevalent, whether these occur in the temperate or tropical zones...
- Molecular characterization of antifolates resistance-associated genes, (dhfr and dhps) in Plasmodium vivax isolates from the Middle East
Sedigheh Zakeri
Malaria and Vector Research Group MVRG, Biotechnology Research Center, Institut Pasteur of Iran, Tehran, Iran
Malar J 8:20. 2009
..vivax infections are often exposed to sulphadoxine-pyrimethamine (SP). In the present study, the frequency distribution of mutations associated to SP resistance was investigated in pvdhfr and pvdhps genes from field isolates...
- Molecular epidemiology of leptospirosis in northern Iran by nested polymerase chain reaction/restriction fragment length polymorphism and sequencing methods
Sedigheh Zakeri
Malaria and Vector Research Group MVRG, Biotechnology Research Center, Institute Pasteur of Iran, Tehran, Iran
Am J Trop Med Hyg 82:899-903. 2010
..Therefore, it is important to increase attention about this disease among physicians and to strengthen laboratory capacity for its diagnosis in infected patients in Iran...
- Molecular characterization of Plasmodium vivax clinical isolates in Pakistan and Iran using pvmsp-1, pvmsp-3alpha and pvcsp genes as molecular markers
Sedigheh Zakeri
Malaria and Vector Research Group, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
Parasitol Int 59:15-21. 2010
..6% for Pakistani and Iranian P. vivax isolates, respectively. These results indicate an extensive diversity in the P. vivax populations in both studies...
- High prevalence of the 437G mutation associated with sulfadoxine resistance among Plasmodium falciparum clinical isolates from Iran, three years after the introduction of sulfadoxine-pyrimethamine
Sedigheh Zakeri
Malaria and Vector Research Group, Biotechnology Research Center, Pasteur Institute of Iran, Pasteur Avenue, Tehran, Iran
Int J Infect Dis 14:e123-8. 2010
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- Genetic structure of Plasmodium vivax isolates from two malaria endemic areas in Afghanistan
Sedigheh Zakeri
Malaria and Vector Research Group, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
Acta Trop 113:12-9. 2010
..PCR/RFLP products with Hha I and Alu I were detected 52 and 38 distinct variants among Nangarhar and Herat isolates, respectively. These results strongly indicate that the P. vivax populations in Afghanistan are highly diverse...
- Leptospira wolffii, a potential new pathogenic Leptospira species detected in human, sheep and dog
Sedigheh Zakeri
Biotechnology Research Center, Institute Pasteur of Iran, Pasteur Avenue, P O Box 1316943551, Tehran, Iran
Infect Genet Evol 10:273-7. 2010
..In addition, this species can be potentially pathogenic to human and probably animal hosts. A large epidemiology survey would be needed to define the presence and the prevalence of this species in global endemic regions...
- Prevalence of mutations associated with antimalarial drugs in Plasmodium falciparum isolates prior to the introduction of sulphadoxine-pyrimethamine as first-line treatment in Iran
Sedigheh Zakeri
Malaria and Vector Research Group MVRG, Biotechnology Research Center, Institut Pasteur of Iran, Pasteur Avenue, PO BOX 1316943551, Tehran, Iran
Malar J 6:148. 2007
..This work was carried out to assess the patterns and prevalence of resistance to chloroquine (CQ) and sulphadoxine-pyrimethamine (SP) in Iran...
- Restricted T-cell epitope diversity in the circumsporozoite protein from Plasmodium falciparum populations prevalent in Iran
Sedigheh Zakeri
Malaria Research Group, Biotechnology Department, Pasteur Institute of Iran, Tehran, Iran
Am J Trop Med Hyg 76:1046-51. 2007
..Furthermore, they sustain the notion that protective responses induced by RTS,S vaccination are not strain-specific...
- Multiple genotypes of the merozoite surface proteins 1 and 2 in Plasmodium falciparum infections in a hypoendemic area in Iran
Sedigheh Zakeri
Malaria Research Group, Pasteur Institute of Iran, Tehran, Iran
Trop Med Int Health 10:1060-4. 2005
..A situation of higher transmission in this area may be emerging, possibly because of reduced efficacy of first-line treatments...
- Genetic variation of TLR-4, TLR-9 and TIRAP genes in Iranian malaria patients
Sedigheh Zakeri
Malaria and Vector Research Group, Biotechnology Research Center, Institut Pasteur Iran, Tehran, Iran, Pasteur Avenue, POBOX 1316943551, Tehran, Iran
Malar J 10:77. 2011
....
- Population structure analysis of Plasmodium vivax in areas of iran with different malaria endemicity
Sedigheh Zakeri
Malaria Research Group, Biotechnology Department, Pasteur Institute of Iran, Tehran, Iran
Am J Trop Med Hyg 74:394-400. 2006
..This high degree of conservation reinforces the potential of this polypeptide fragment for inclusion in synthetic vaccines being developed against P. vivax...
- Circumsporozoite protein gene diversity among temperate and tropical Plasmodium vivax isolates from Iran
Sedigheh Zakeri
Malaria Research Group, Biotechnology Department, Pasteur Institute of Iran, Tehran, Iran
Trop Med Int Health 11:729-37. 2006
..This large-scale survey of parasite diversity in the Eastern Mediterranean Region provides a set of baseline data suitable for future molecular epidemiological studies of P. vivax...
- Merozoite surface protein-3alpha is a reliable marker for population genetic analysis of Plasmodium vivax
Sedigheh Zakeri
Malaria Research Group, Biotechnology Department, Pasteur Institute of Iran, Pasteur Avenue, Tehran, Iran
Malar J 5:53. 2006
..In this study, msp-3alpha was compared and assessed with msp-1 marker in order to find whether msp-3alpha is a reliable genetic marker for P. vivax population studies...
- Identification and typing of Cameroonian isolates of P. malariae using monoclonal antibodies against P. brasilianum
Sedigheh Zakeri
Wolfson Unit of Tropical Immunology, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool L3 5QA, United Kingdom
Acta Trop 99:97-101. 2006
..malariae parasites. In addition our results for the first time provide evidence of considerable antigenic diversity of clinical P. malariae isolates in Cameroon...
- Non-variant specific antibody responses to the C-terminal region of merozoite surface protein-1 of Plasmodium falciparum (PfMSP-1(19)) in Iranians exposed to unstable malaria transmission
Sedigheh Zakeri
Malaria and Vector Research Group MVRG, Biotechnology Research Center, Institut Pasteur Iran, Pasteur Avenue, PO ONL001740209 BOX 1316943551, Tehran, Iran
Malar J 9:257. 2010
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- Detection of mixed Plasmodium falciparum & P. vivax infections by nested-PCR in Pakistan, Iran & Afghanistan
Sedigheh Zakeri
Malaria and Vector Research Group MVRG, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
Indian J Med Res 132:31-5. 2010
..In this investigation, malaria diagnosis using molecular method was used to assess the transmission pattern of malaria parasite in three malaria endemic regions: Afghanistan, Iran and Pakistan...
- Molecular assessment of dhfr/dhps mutations among Plasmodium vivax clinical isolates after introduction of sulfadoxine/pyrimethamine in combination with artesunate in Iran
Mandana Afsharpad
Malaria and Vector Research Group MVRG, Biotechnology Research Center BRC, Pasteur Institute of Iran, Pasteur Avenue, P O Box 1316943551, Tehran, Iran
Infect Genet Evol 12:38-44. 2012
..vivax isolates in Iran in near future...
- Cloning, expression and transmission-blocking activity of anti-PvWARP, malaria vaccine candidate, in Anopheles stephensi mysorensis
Saber Gholizadeh
Department of Medical Entomology, School of Public Health, Tehran University of Medical Science, Tehran, Iran, PO Box 6446 14155, Tehran, Iran
Malar J 9:158. 2010
..Ookinete-secreted protein, Plasmodium vivax von Willebrand factor A domain-related protein (PvWARP), is a candidate for malaria transmission-blocking vaccines (TBVs)...
- Molecular monitoring of Plasmodium falciparum resistance to antimalarial drugs after adoption of sulfadoxine-pyrimethamine plus artesunate as the first line treatment in Iran
Mandana Afsharpad
Malaria and Vector Research Group, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
Acta Trop 121:13-8. 2012
..In conclusion, the present findings suggest that in Iran, SP is still effective for the treatment of uncomplicated falciparum malaria as a partner drug of Artemisinin Combination Therapy (ACT) in this region...
- Plasmodium falciparum: sequence analysis of the gene encoding the C-terminus region of the merozoite surface protein-1, a potential malaria vaccine antigen, in Iranian clinical isolates
Akram Abouie Mehrizi
Malaria and Vector Research Group, Biotechnology Research Center, Pasteur Institute of Iran, Pasteur Avenue, PO BOX 1316943551, Tehran, Iran
Exp Parasitol 118:378-85. 2008
..In summary, result of this study indicates limited antigenic diversity, and thus support the potential utility of the C-terminal region of PfMSP-1 in designing polyvalent vaccine constructs...
- Identification, sequence analysis, and comparative study on GSTe2 insecticide resistance gene in three main world malaria vectors: Anopheles stephensi, Anopheles culicifacies, and Anopheles fluviatilis
Navid Dinparast Djadid
Malaria Research Group, Biotechnology Department, Pasteur Institute of Iran, Tehran, Iran
J Med Entomol 43:1171-7. 2006
..gambiae. These data may be useful for implementation and evaluation of malaria control programs in aspects of population genetics and molecular resistance...
- IgG subclasses pattern and high-avidity antibody to the C-terminal region of merozoite surface protein 1 of Plasmodium vivax in an unstable hypoendemic region in Iran
Akram Abouie Mehrizi
Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
Acta Trop 112:1-7. 2009
..These results could help to understand the interactions between the host and P. vivax parasite in development of MSP-1(19)-based vaccine...
- Analysis of von Willebrand factor A domain-related protein (WARP) polymorphism in temperate and tropical Plasmodium vivax field isolates
Saber Gholizadeh
Biotechnology Research Center BRC, Pasteur Institute of Iran PII, Tehran, Iran
Malar J 8:137. 2009
..Here, the sequence analysis of an extra-cellular malaria protein expressed in ookinetes, named von Willebrand factor A domain-related protein (WARP), is reported in 91 Plasmodium vivax isolates circulating in different regions of Iran...
- In vitro antiplasmodial and phytochemical study of five Artemisia species from Iran and in vivo activity of two species
Ali Ramazani
Malaria and Vector Research Group, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
Parasitol Res 107:593-9. 2010
..28% and 83.28%, respectively, but we could not detect artemisinin in all plants studied in this research. The antiplasmodial property of these two herbs is possibly related to essential oils that present in high amounts in their extracts...
- Molecular identification of Palearctic members of Anopheles maculipennis in northern Iran
Navid D Djadid
Malaria Research Group, Biotechnology Department, Institut Pasteur of Iran, Tehran, Iran
Malar J 6:6. 2007
..The current study has been designed in order to provide further evidence on the status of species composition and to identify more accurately the members of the maculipennis complex in northern Iran...
- IgG subclass antibodies to three variants of Plasmodium falciparum merozoite surface protein-1 (PfMSP-1(19)) in an area with unstable malaria transmission in Iran
Akram Abouie Mehrizi
Malaria and Vector Research Group, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
Acta Trop 119:84-90. 2011
..These results could support the design of a universal PfMSP-1(19)-based vaccine...
- Antibody responses and avidity of naturally acquired anti-Plasmodium vivax Duffy binding protein (PvDBP) antibodies in individuals from an area with unstable malaria transmission
Sedigheh Zakeri
Malaria and Vector Research Group MVRG, Biotechnology Research Center BRC, Pasteur Institute of Iran, Tehran, Iran
Am J Trop Med Hyg 84:944-50. 2011
..To sum up, the results provided additional data in our understanding of blood-stage immunity to PvDBP, supporting the rational development of an effective blood-stage vaccine based on this antigen...
- A simple and rapid nested polymerase chain reaction-restriction fragment length polymorphism technique for differentiation of pathogenic and nonpathogenic Leptospira spp
Navid Dinparast Djadid
Biotechnology Research Center, Pasteur Institute of Iran, Tehran
Diagn Microbiol Infect Dis 63:251-6. 2009
..The nested PCR-RFLP assay developed in this study fulfills this requirement in the early stage of infection...
- Genetic analysis of rDNA-ITS2 and RAPD loci in field populations of the malaria vector, Anopheles stephensi (Diptera: Culicidae): implications for the control program in Iran
Navid Dinparast Djadid
Malaria Research Group, Biotechnology Department, Pasteur Institute of Iran, Tehran, Iran
Acta Trop 97:65-74. 2006
..This data will serve as first report on the sequence of rDNA-ITS2 and a microsatellite-containing RAPD region, which could be used for species-specific diagnosis and differentiation of urban and rural populations in An. stephensi...
- Monitoring pyrethroid insecticide resistance in major malaria vector Anopheles culicifacies: comparison of molecular tools and conventional susceptibility test
Navid Dinparast Djadid
Biotechnology Dept, Pasteur Institute of Iran, Tehran
Iran Biomed J 11:169-76. 2007
..So far, resistance to DDT, dieldrin, malathion and partial tolerance to pyrethroids has been reported in An. stephensi, but nothing confirmed on resistance status of An. culicifacies in Iran...
- Detection of malaria parasites by nested PCR in south-eastern, Iran: evidence of highly mixed infections in Chahbahar district
Sedigheh Zakeri
Malaria Research Group MRG, Biotechnology Department, Pasteur Institute of Iran, Tehran, Iran
Malar J 1:2. 2002
..Rapid diagnosis and correct treatment of cases are the main objectives of control programs in malaria-endemic areas...
- Sequence heterogeneity of the merozoite surface protein-1 gene (MSP-1) of Plasmodium vivax wild isolates in southeastern Iran
Sedigheh Zakeri
Department of Biotechnology, Malaria Research Unit, Pasteur Institute of Iran, P O Box 13164, Tehran, Iran
Acta Trop 88:91-7. 2003
..Thus, this study provides preliminary evidence of sequence heterogeneity in the Iranian P. vivax population...
- Identification of the midgut microbiota of An. stephensi and An. maculipennis for their application as a paratransgenic tool against malaria
Navid Dinparast Djadid
Malaria and Vector Research Group, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
PLoS ONE 6:e28484. 2011
..The presented data strongly encourage further investigations, to verify the potential role of the detected bacteria for the malaria control in Iran and neighboring countries...
- High prevalence of double Plasmodium falciparum dhfr mutations at codons 108 and 59 in the Sistan-Baluchistan province, Iran
Sedigheh Zakeri
J Infect Dis 187:1828-9. 2003
