Research Topics
Genomes and GenesSpecies | Clement A StanyonSummaryAffiliation: Wayne State University Country: USA Publications
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Detail Information
Publications
A Drosophila protein-interaction map centered on cell-cycle regulatorsClement A Stanyon
Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, 540 E, Canfield Avenue, Detroit, MI 48201, USA
Genome Biol 5:R96. 2004..Although these data are a valuable resource for insights into protein networks, they cover only a fraction of the expected number of interactions...
A strategy for constructing large protein interaction maps using the yeast two-hybrid system: regulated expression arrays and two-phase matingJinhui Zhong
Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
Genome Res 13:2691-9. 2003..We also present a three-dimensional pooling scheme to further increase the efficiency of large-scale two-hybrid analyses...
Regulated expression of proteins in yeast using the MAL61-62 promoter and a mating scheme to increase dynamic rangeRussell L Finley
Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, 540 East Canfield Avenue, Detroit, MI 48201, USA
Gene 285:49-57. 2002..We demonstrate a two-hybrid system in which MAL62p is used in conjunction with the yeast GAL1 promoter to independently regulate expression of both hybrid proteins, and to allow detection of interactions involving toxic proteins...
Simultaneous cloning of open reading frames into several different expression vectorsClement A Stanyon
Wayne State University, Detroit, MI, USA
Biotechniques 35:520-2, 524-6. 2003..Using this system to produce multiple expression constructs simultaneously could greatly facilitate high-throughput subcloning and proteomic studies...
