Research Topics
Genomes and Genes
| L J SandellSummaryAffiliation: Washington University School of Medicine Country: USA Publications
Research Grants
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Detail Information
Publications
Developmental patterns of cartilageL J Sandell
Department of Orthopaedic Surgery, Barnes Jewish Hospital at Washington University School of Medicine, 216 South Kingshighway, St Louis, Missouri 63110, USA
Front Biosci 4:D731-42. 1999..This will then be compared with the limited information currently known about the molecular aspects of osteoarthritis...- Anabolic factors in degenerative joint diseaseL J Sandell
Washington University Medical School, Department of Orthopaedic Surgery, at Barnes Jewish Hospital, 660 S Euclid, Box 8233, St Louis, MO 63110, USA
Curr Drug Targets 8:359-65. 2007..We will also be interested in viewing the development of degenerative arthritis as a continuum from functionally normal tissue to degeneration... - Articular cartilage and changes in arthritis. An introduction: cell biology of osteoarthritisL J Sandell
Department of Orthopaedic Surgery, Washington University School of Medicine, 216 South Kingshighway, St Louis, MO 63110, USA
Arthritis Res 3:107-13. 2001..Proliferation plays a role in forming characteristic chondrocyte clusters near the surface, while apoptosis probably occurs primarily in the calcified cartilage... - Type IIA procollagen in development of the human intervertebral disc: regulated expression of the NH(2)-propeptide by enzymic processing reveals a unique developmental pathwayY Zhu
Department of Orthopaedic Surgery at Barnes-Jewish Hospital, Washington University, St. Louis, Missouri 63110, USA
Dev Dyn 220:350-62. 2001..This developmental pattern may prove important for disc regeneration... - Cytokine regulation of cartilage-derived retinoic acid-sensitive protein (CD-RAP) in primary articular chondrocytes: suppression by IL-1, bfGF, TGFbeta and stimulation by IGF-1S Kondo
Department of Orthopaedic Surgery, Washington University School of Medicine at Barnes-Jewish Hospital, St. Louis, MO 63110, USA
J Orthop Res 19:712-9. 2001..These data indicate that CD-RAP expression can be regulated by cytokines known to influence chondrocyte metabolism and that IGF-1 up-regulates CD-RAP gene expression through a transcriptional mechanism... - Exuberant expression of chemokine genes by adult human articular chondrocytes in response to IL-1betaL J Sandell
Department of Orthopaedic Surgery, Washington University School of Medicine at Barnes Jewish Hospital, St Louis, MO 63110, United States
Osteoarthritis Cartilage 16:1560-71. 2008..To provide a more complete picture of the effect of interleukin-1 beta (IL-1beta) on adult human articular chondrocyte gene expression, in contrast to the candidate gene approach... - Expression of type II procollagens during development of the human intervertebral discA McAlinden
Department of Orthopaedic Surgery, Washington University at Barnes-Jewish Hospital, Mail Stop 90-34-674, 216 South Kingshighway, St. Louis, MO 63110, USA
Biochem Soc Trans 30:831-8. 2002..We aim to explore this mechanism further... - Cell biology of osteoarthritis: the chondrocyte's response to injuryN Fukui
Department of Orthopaedic Surgery, Washington University School of Medicine, Barnes-Jewish Hospital, Mail Stop 90-34-674, 216 South Kingshighway, St. Louis, MO 63110, USA
Curr Rheumatol Rep 3:496-505. 2001..Eventually, the newly synthesized and activated catabolic enzymes degrade the matrix components. This review presents the cellular and molecular mechanisms that account for this activity and provides some possible solutions... - Type IIA procollagen containing the cysteine-rich amino propeptide is deposited in the extracellular matrix of prechondrogenic tissue and binds to TGF-beta1 and BMP-2Y Zhu
Washington University School of Medicine, Department of Orthopedic Surgery, St Louis, Missouri 63110, USA
J Cell Biol 144:1069-80. 1999..Taken together, these results suggest that the NH2-propeptide of type IIA procollagen could function in the extracellular matrix distribution of bone morphogenetic proteins in chondrogenic tissue... - The 2.2-kb promoter of cartilage-derived retinoic acid-sensitive protein controls gene expression in cartilage and embryonic mammary buds of transgenic miceW F Xie
Washington University School of Medicine, Department of Orthopaedic Surgery, Yalem Research Building, Room 704, 216 South Kingshighway, St Louis, MO 63110, USA
Matrix Biol 19:501-9. 2000..The finding of transient CD-RAP expression in mammary buds suggests that it may play a role in the organogenesis of mammary glands... - Distribution of the transcription factors Sox9, AP-2, and [delta]EF1 in adult murine articular and meniscal cartilage and growth plateSherri R Davies
Washington University School of Medicine at Barnes Jewish Hospital, Department of Orthopaedic Surgery, St Louis, Missouri 63110, USA
J Histochem Cytochem 50:1059-65. 2002..These immunohistochemical results are the first to localize both [delta]EF1 and AP-2 to adult articular, meniscal, and growth plate cartilages and provide in vivo correlation of previous molecular biological studies... - Alternative splicing of type II procollagen exon 2 is regulated by the combination of a weak 5' splice site and an adjacent intronic stem-loop cis elementAudrey McAlinden
Department of Orthopaedic Surgery, Washington University School of Medicine, St Louis, Missouri 63110, USA
J Biol Chem 280:32700-11. 2005..This is the first report identifying key cis elements in the COL2A1 gene that modulate the cell type-specific alternative splicing switch of exon 2 during cartilage development... - Site-1 protease is essential for endochondral bone formation in miceDebabrata Patra
Department of Orthopaedic Surgery, Washington University School of Medicine at Barnes Jewish Hospital, St Louis, MO 63110, USA
J Cell Biol 179:687-700. 2007..These data suggest that S1P activity is necessary for a specialized ER stress response required by chondrocytes for the genesis of normal cartilage and thus endochondral ossification... - Computational identification and functional validation of regulatory motifs in cartilage-expressed genesSherri R Davies
Department of Orthopaedic Surgery, Washington University School of Medicine, St Louis, Missouri 63110, USA
Genome Res 17:1438-47. 2007..Mutational analyses suggest that this motif binds a repressive factor that regulates basal levels of the COL2A1 promoter... - Characteristics of the rat supraspinatus tendon during tendon-to-bone healing after acute injuryLeesa M Galatz
Department of Orthopaedic Research, Washington University School of Medicine, Barnes Jewish Hospital, 660 S Euclid Avenue, Campus Box 8233, St Louis, Missouri 63110, USA
J Orthop Res 24:541-50. 2006..Repair sites remained unorganized histologically and biomechanically inferior in comparison to previously described uninjured insertion sites... - Pro-inflammatory cytokine tumor necrosis factor-alpha induces bone morphogenetic protein-2 in chondrocytes via mRNA stabilization and transcriptional up-regulationNaoshi Fukui
Department of Pathomechanisms, Clinical Research Center, National Hospital Organization Sagamihara Hospital, Sagamihara, Kanagawa, and Department of Orthopaedic Surgery, Faculty of Medicine, The University of Tokyo, Japan
J Biol Chem 281:27229-41. 2006.... - Medial collateral ligament healing in macrophage metalloelastase (MMP-12)-deficient miceRick W Wright
Department of Orthopaedic Surgery, Washington University School of Medicine at Barnes Jewish Hospital, St Louis, Missouri, USA
J Orthop Res 24:2106-13. 2006..05 by Tukey post hoc test). We conclude MME is important in the multifactorial cascade of knee MCL injury healing, showing significant differences in both the early inflammatory and in the matrix tissue synthesis phases... - Smoking delays chondrogenesis in a mouse model of closed tibial fracture healingHossam B El-Zawawy
Department of Orthopaedic Surgery, Washington University School of Medicine at Barnes-Jewish Hospital, 660 S. Euclid Avenue, Campus Box 8233, St. Louis, Missouri 63110, USA
J Orthop Res 24:2150-8. 2006..This study represents, to our knowledge, the first analysis of molecular and cellular mechanisms of healing in a smoking mouse fracture model... - Effects of cigarette smoking on early medial collateral ligament healing in a mouse modelCorey S Gill
Department of Orthopaedic Surgery, Washington University School of Medicine at Barnes Jewish Hospital, 660 S Euclid Ave, Box 8233, St Louis, Missouri 63110, USA
J Orthop Res 24:2141-9. 2006..We conclude that the decreased cellular density and type I collagen expression in the injured ligament of mice exposed to smoke begin to provide a cellular and molecular basis for delayed or deficient early healing in these animals... - Nuclear protein TIA-1 regulates COL2A1 alternative splicing and interacts with precursor mRNA and genomic DNAAudrey McAlinden
Department of Orthopaedic Surgery, Washington University School of Medicine, St Louis, Missouri 63110, USA
J Biol Chem 282:24444-54. 2007..This suggests a potentially novel, dual role for TIA-1 in shuttling between DNA and RNA ligands to co-regulate COL2A1 expression at the level of transcription and pre-mRNA alternative splicing... - Transcriptional Co-activators CREB-binding protein/p300 increase chondrocyte Cd-rap gene expression by multiple mechanisms including sequestration of the repressor CCAAT/enhancer-binding proteinToshihiro Imamura
Department of Orthopaedic Surgery, Washington University School of Medicine at Barnes Jewish Hospital, St Louis, Missouri 63110, USA
J Biol Chem 280:16625-34. 2005..This new paradigm is likely generally applicable to cartilage genes as Col2a1 cartilage collagen gene responds similarly... - Solution structure and dynamics of a prototypical chordin-like cysteine-rich repeat (von Willebrand Factor type C module) from collagen IIAJoanne M O'Leary
Division of Structural Biology, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK
J Biol Chem 279:53857-66. 2004.... - Regional differences in chondrocyte metabolism in osteoarthritis: a detailed analysis by laser capture microdissectionNaoshi Fukui
National Hospital Organization Sagamihara Hospital, Sagamihara, Japan
Arthritis Rheum 58:154-63. 2008..To determine the change in metabolic activity of chondrocytes in osteoarthritic (OA) cartilage, considering regional difference and degree of cartilage degeneration... - What's new in orthopaedic researchMatthew J Silva
Department of Orthopaedic Surgery, Washington University School of Medicine at Barnes-Jewish Hospital, St. Louis, MO 63110, USA
J Bone Joint Surg Am 84:1490-6. 2002 - Upregulation of Runx2 and Osterix during in vitro chondrogenesis of human adipose-derived stromal cellsJason T Rich
Department of Otolaryngology Head and Neck Surgery, Washington University School of Medicine St Louis, MO 63110, USA
Biochem Biophys Res Commun 372:230-5. 2008..We conclude that alternative strategies are required to induce efficient articular chondrocyte differentiation in order for HADSCs to be of clinical use in cartilage tissue engineering... - Uncoupling of type II collagen synthesis and degradation predicts progression of joint damage in patients with knee osteoarthritisPatrick Garnero
INSERM Unit 403, E Herriot Hospital and Synarc, Pavillon F, 69437 Lyon Cedex 03, France
Arthritis Rheum 46:2613-24. 2002.... - CCAAT/enhancer-binding proteins beta and delta mediate the repression of gene transcription of cartilage-derived retinoic acid-sensitive protein induced by interleukin-1 betaKen Okazaki
Department of Orthopaedic Surgery, Washington University School of Medicine at Barnes Jewish Hospital, St Louis, Missouri 63110, USA
J Biol Chem 277:31526-33. 2002.... - Egr-1 mediates transcriptional repression of COL2A1 promoter activity by interleukin-1betaLujian Tan
Rheumatology Division, Beth Israel Deaconess Medical Center and New England Baptist Bone and Joint Institute, Harvard Institutes of Medicine, Boston, Massachusetts 02115, USA
J Biol Chem 278:17688-700. 2003.... - Stimulation of BMP-2 expression by pro-inflammatory cytokines IL-1 and TNF-alpha in normal and osteoarthritic chondrocytesNaoshi Fukui
J Bone Joint Surg Am 85:59-66. 2003..Furthermore, the pro-inflammatory cytokines IL-1beta and TNF-alpha, known to be present in synovium and cartilage of patients with osteoarthritis, stimulate the production of active BMP-2... - Tissue-restricted expression of the Cdrap/Mia gene within a conserved multigenic housekeeping locusSherri R Davies
Department of Orthopaedic Surgery, Washington University at Barnes Jewish Hospital, Mail Stop 90 34 674, 216 South Kingshighway, St Louis, MO 63110, USA
Genomics 83:667-78. 2004..The tissue-restricted expression of Cdrap/Mia, located between two housekeeping genes, provides a distinctive model for restricted transcriptional regulation from a multigenic locus... - Regulation of protein diversity by alternative pre-mRNA splicing with specific focus on chondrogenesisAudrey McAlinden
Department of Orthopaedic Surgery, Washington University School of Medicine, St. Louis, Missouri, USA
Birth Defects Res C Embryo Today 72:51-68. 2004.... - Alterations in apoptosis and epithelial-mesenchymal transformation in an in vitro cleft palate modelJudith M Gurley
Division of Plastic Surgery, Department of Surgery, St Louis Children s Hospital, St Louis, MO 63110, USA
Plast Reconstr Surg 113:907-14. 2004..Perhaps in utero manipulation of intershelf spacing or epithelial-mesenchymal transformation and/or apoptosis could reverse the clefting paradigm... - Modern molecular analysis of a traditional disease: progression in osteoarthritisLinda J Sandell
Arthritis Rheum 56:2474-7. 2007 - A novel tumor necrosis factor alpha-responsive CCAAT/enhancer binding protein site regulates expression of the cartilage-derived retinoic acid-sensitive protein gene in cartilageToshihiro Imamura
Washington University, School of Medicine at Barnes Jewish Hospital, St Louis, Missouri 63110, USA
Arthritis Rheum 58:1366-76. 2008..The aim of this study was to investigate novel TNFalpha-mediated mechanisms that regulate the expression of cd-rap... - A promoter element of the CD-RAP gene is required for repression of gene expression in non-cartilage tissues in vitro and in vivoKen Okazaki
Department of Orthopaedic Surgery, Washington University School of Medicine at Barnes Jewish Hospital, St Louis, Missouri 63110, USA
J Cell Biochem 97:857-68. 2006..This is the first report of a functional DNA element necessary for repression in non-cartilage tissues in vivo... - In vivo human Cartilage oligomeric matrix protein (COMP) promoter activityKaren L Posey
Department of Pediatrics, University of Texas Medical School at Houston, 6431 Fannin, 77030, USA
Matrix Biol 24:539-49. 2005..Our findings indicate that the elements required for chondrocyte-specific expression lie within 375 bp of the translational start site, while DNA enhancer elements are located between 1.0 to 1.7 kb... - Processing of type II procollagen amino propeptide by matrix metalloproteinasesNaoshi Fukui
Department of Orthopaedic Surgery, Barnes Jewish Hospital at Washington University School of Medicine, St Louis, Missouri 63110, USA
J Biol Chem 277:2193-201. 2002.... - Alpha-helical coiled-coil oligomerization domains are almost ubiquitous in the collagen superfamilyAudrey McAlinden
Department of Orthopedic Surgery, Washington University School of Medicine, Barnes-Jewish Hospital, St. Louis, MO 63110, USA
J Biol Chem 278:42200-7. 2003..These observations suggest that coiled-coil oligomerization domains are widely used in the assembly of collagens and collagen-like proteins... - Trimerization of the amino propeptide of type IIA procollagen using a 14-amino acid sequence derived from the coiled-coil neck domain of surfactant protein DAudrey McAlinden
Department of Orthopaedic Surgery, Washington University School of Medicine at Barnes-Jewish Hospital, 660 South Euclid Avenue, St. Louis, MO 63110, USA
J Biol Chem 277:41274-81. 2002.... - Development and clinical application in arthritis of a new immunoassay for serum type IIA procollagen NH2 propeptideJean-Charles Rousseau
INSERM Unit 403, , Lyon, France
Methods Mol Med 101:25-37. 2004..0001) and 35% (p < 0.001), respectively, suggesting that type IIA collagen synthesis is altered in these arthritic diseases. The measurement of serum PIIANP may be useful for the clinical investigation of patients with joint diseases...
Research Grants
- Tissue Engineering in Musculoskeletal Clinical PracticeLinda Sandell; Fiscal Year: 2003..Lastly, worldwide current and future clinical trials will be discussed. ..
- 2005 Biology and Pathology of Cartilage Gordon ConferenceLinda Sandell; Fiscal Year: 2005..The conference will be held at the II Ciocco, Barga, Italy on June 5-9, 2005. ..