David A Rozak

Summary

Affiliation: Walter Reed Army Medical Center
Country: USA

Publications

  1. ncbi G148-GA3: a streptococcal virulence module with atypical thermodynamics of folding optimally binds human serum albumin at physiological temperatures
    David A Rozak
    Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, Rockville, MD 20850, USA
    Biochim Biophys Acta 1753:226-33. 2005
  2. ncbi CpG oligodeoxyribonucleotides protect mice from Burkholderia pseudomallei but not Francisella tularensis Schu S4 aerosols
    David A Rozak
    Bacteriology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland, USA
    J Immune Based Ther Vaccines 8:2. 2010
  3. ncbi Simplicity, function, and legibility in an enhanced ambigraphic nucleic acid notation
    David A Rozak
    Bacteriology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702, USA
    Biotechniques 44:811-3. 2008
  4. ncbi Using offset recombinant polymerase chain reaction to identify functional determinants in a common family of bacterial albumin binding domains
    David A Rozak
    Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, 9600 Gudelsky Drive, Rockville, Maryland 20850, USA
    Biochemistry 45:3263-71. 2006
  5. ncbi The practical and pedagogical advantages of an ambigraphic nucleic acid notation
    David A Rozak
    Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, Rockville, Maryland, USA
    Nucleosides Nucleotides Nucleic Acids 25:807-13. 2006
  6. ncbi Structure, dynamics, and stability variation in bacterial albumin binding modules: implications for species specificity
    Yanan He
    Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, 9600 Gudelsky Drive, Rockville, Maryland 20850, USA
    Biochemistry 45:10102-9. 2006
  7. ncbi An artificially evolved albumin binding module facilitates chemical shift epitope mapping of GA domain interactions with phylogenetically diverse albumins
    Yanan He
    Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, Rockville, MD 20850, USA
    Protein Sci 16:1490-4. 2007
  8. ncbi Directed evolution of highly homologous proteins with different folds by phage display: implications for the protein folding code
    Patrick A Alexander
    Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, 9600 Gudelsky Drive, Rockville, Maryland 20850, USA
    Biochemistry 44:14045-54. 2005
  9. ncbi Exogenous Yersinia pestis quorum sensing molecules N-octanoyl-homoserine lactone and N-(3-oxooctanoyl)-homoserine lactone regulate the LcrV virulence factor
    H Carl Gelhaus
    United States Army Medical Institute of Infectious Diseases, Bacteriology Division, 1425 Porter St, 21702 Ft Detrick, MD, USA
    Microb Pathog 46:283-7. 2009
  10. ncbi Offset recombinant PCR: a simple but effective method for shuffling compact heterologous domains
    David A Rozak
    Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, 9600 Gudelsky Drive, Rockville, MD 20850, USA
    Nucleic Acids Res 33:e82. 2005

Collaborators

Detail Information

Publications10

  1. ncbi G148-GA3: a streptococcal virulence module with atypical thermodynamics of folding optimally binds human serum albumin at physiological temperatures
    David A Rozak
    Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, Rockville, MD 20850, USA
    Biochim Biophys Acta 1753:226-33. 2005
    ..4 x 10 7 M(-1). Analysis of G148-GA3 thermodynamics suggests that the domain experiences atypically small per residue changes in structural dynamics and heat capacity while transiting between folded and unfolded states...
  2. ncbi CpG oligodeoxyribonucleotides protect mice from Burkholderia pseudomallei but not Francisella tularensis Schu S4 aerosols
    David A Rozak
    Bacteriology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland, USA
    J Immune Based Ther Vaccines 8:2. 2010
    ..Our results, which contrast with earlier F. tularensis LVS studies, highlight potential differences in Francisella species pathogenesis and underscore the need to evaluate immunotherapies against human pathogenic species...
  3. ncbi Simplicity, function, and legibility in an enhanced ambigraphic nucleic acid notation
    David A Rozak
    Bacteriology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702, USA
    Biotechniques 44:811-3. 2008
    ....
  4. ncbi Using offset recombinant polymerase chain reaction to identify functional determinants in a common family of bacterial albumin binding domains
    David A Rozak
    Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, 9600 Gudelsky Drive, Rockville, Maryland 20850, USA
    Biochemistry 45:3263-71. 2006
    ..Thermodynamic data for the most common phage-selected mutant suggest that domain-stabilizing mutations substantially improved GA binding for both species of albumin...
  5. ncbi The practical and pedagogical advantages of an ambigraphic nucleic acid notation
    David A Rozak
    Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, Rockville, Maryland, USA
    Nucleosides Nucleotides Nucleic Acids 25:807-13. 2006
    ....
  6. ncbi Structure, dynamics, and stability variation in bacterial albumin binding modules: implications for species specificity
    Yanan He
    Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, 9600 Gudelsky Drive, Rockville, Maryland 20850, USA
    Biochemistry 45:10102-9. 2006
    ..Biol. 316, 1083-1099), because PSD-1 binds the phylogenetically diverse HSA and GPSA even more tightly than G148-GA3 but is less flexible. The structural basis for albumin-binding specificity is analyzed in light of these new results...
  7. ncbi An artificially evolved albumin binding module facilitates chemical shift epitope mapping of GA domain interactions with phylogenetically diverse albumins
    Yanan He
    Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, Rockville, MD 20850, USA
    Protein Sci 16:1490-4. 2007
    ..The employment of artificially evolved domains may be generally useful in NMR structural studies of other protein-protein complexes...
  8. ncbi Directed evolution of highly homologous proteins with different folds by phage display: implications for the protein folding code
    Patrick A Alexander
    Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, 9600 Gudelsky Drive, Rockville, Maryland 20850, USA
    Biochemistry 44:14045-54. 2005
    ..Further, the thermodynamic linkage between folding and binding is used to assess the propensity of one sequence to adopt two unique folds...
  9. ncbi Exogenous Yersinia pestis quorum sensing molecules N-octanoyl-homoserine lactone and N-(3-oxooctanoyl)-homoserine lactone regulate the LcrV virulence factor
    H Carl Gelhaus
    United States Army Medical Institute of Infectious Diseases, Bacteriology Division, 1425 Porter St, 21702 Ft Detrick, MD, USA
    Microb Pathog 46:283-7. 2009
    ..DNA microarray analysis shows 10 additional T3SS genes are consistently down-regulated by C8 or oxo-C8. This is the first report demonstrating that AHLs regulate Y. pestis virulence factor expression...
  10. ncbi Offset recombinant PCR: a simple but effective method for shuffling compact heterologous domains
    David A Rozak
    Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, 9600 Gudelsky Drive, Rockville, MD 20850, USA
    Nucleic Acids Res 33:e82. 2005
    ....